ライフサイエンスコーパス: basophil

1 IL-31RA and OSMR were expressed on human basophils.

2 pression of CD200R on Th2 and ILC2 cells and basophils.

3 epsilonRI expression on mast cells (MCs) and basophils.

4 and FcRgamma>mast cells>IgE and FcepsilonRI>basophils.

5 would weakly upregulate CD203c expression on basophils.

6 y IgE receptor FcepsilonRI on mast cells and basophils.

7 tions of dendritic cells and 1 population of basophils.

8 duced cytokine secretion among mouse MCs and basophils.

9 alizumab-resistant IgE-Fc fragments on human basophils.

10 c target in IgE receptor (IgER)-cross-linked basophils.

11 und antibodies (IgE, IgG) were quantified on basophils.

12 of IgE-dependent histamine release in human basophils.

13 the others had anti-FcepsilonRI nonreactive basophils.

14 as strong infiltration of CD8(+) T cells and basophils.

15 Analogous experiments cocultured basophils (1-72 h) directly with EC lines.

16 whereas eosinophil degranulation (CD69) and basophil activation (CD203c) were higher in ACS patients

17 11%; P<.001), eNO (86 vs 53 ppb; P<.05) and basophil activation after 2 weeks (CD63 expression 79% v

18 The effect of rfhSP-D on basophil activation and histamine release was measured b

19 uggested that SHR/T could be an indicator of basophil activation and hypersensitivity in vivo.

20 h quantitative RAST-based methods, in ELISA, basophil activation and IgE-facilitated allergen present

21 erapeutic interventions, events like loss of basophil activation and induction of regulatory lymphocy

22 immune responses that included IgE-dependent basophil activation and measurement of serum inhibitory

23 rticipants, along with trends toward reduced basophil activation and peanut-specific TH2 cytokines.

24 were assayed in ELISA and ELISA inhibition, basophil activation and skin prick test.

25 Additionally, changes in basophil activation and specific IgE and IgG4 were asses

26 be comparable to natural Phl p 5 in terms of basophil activation and T-cell reactivity.

27 IgE-allergen complexes induce mast cell and basophil activation and thus immediate allergic inflamma

28 Several methods measuring basophil activation are available.

29 zed, and allergenic activity was assessed by basophil activation assay.

30 Questionnaires, skin prick tests, and basophil activation assays were performed.

31 Allergenic activity was determined using basophil activation assays.

32 e their activity, suggested a unique mode of basophil activation by A549 EC.

33 ren inhibited allergen-induced IgE-dependent basophil activation in a dose-dependent fashion.

34 ted after discovering that LacNAc suppressed basophil activation in cocultures.

35 isolated meat proteins were shown to induce basophil activation in patients with delayed anaphylaxis

36 Levels of the A fumigatus-stimulated basophil activation marker CD203c inversely correlated w

37 We measured expression of basophil activation markers (CD63 and CD203c); the absol

38 Sequential peaks in peripheral blood basophil activation markers were observed (CD107a at 3 h

39 This combination treatment also blocks basophil activation more efficiently than either agent a

40 release might add to our knowledge about the basophil activation properties of moxifloxacin and const

41 Subgroups were analyzed by basophil activation test (BAT) and CAP-FEIA-based cross-

42 The basophil activation test (BAT) has emerged as having sup

43 d by ImmunoCAP, ELISA, cross-inhibition, and basophil activation test (BAT) in patients with yellow j

44 All had a basophil activation test (BAT) with moxifloxacin.

45 This study aimed at comparing basophil activation test (BAT), histamine release assay

46 for allergy diagnostics was investigated by basophil activation test (BAT).

47 nsitization in patients with CF by using the basophil activation test (BAT).

48 gy that can be determined in vitro using the basophil activation test (BAT).

49 dependent fMLP stimulation was determined by basophil activation test in comparison with patients suf

50 inant allergen in the diagnostic procedures (basophil activation test, IgE immunoblot, and experiment

51 e results were obtained in a drug-stimulated basophil activation test.

52 IgE cross-linking capacity was analyzed in a basophil activation test.

53 Other approaches are in development with basophil activation testing being closest to clinical ap

54 s) using finely ground tree-nut solution and basophil activation tests (BAT) were performed.

55 Basophil activation tests (BATs) have promise for resear

56 tory, skin prick tests (SPTs) with IVIP, and basophil activation tests (BATs) in some patients.

57 uantification of specific IgE antibodies and basophil activation tests can also be helpful to establi

58 Skin testing and basophil activation tests were performed using a commerc

59 Basophil activation tests were used to investigate react

60 Skin prick tests and basophil activation tests with N, P, or PM were performe

61 Additional novel diagnostics, such as basophil activation tests, determination of epitope bind

62 ibodies were tested for blocking capacity in basophil activation tests.

63 binding was tested in inhibition ELISAs and basophil activation tests.

64 Basophil activation was evaluated by the mean fluorescen

65 "alternatively activated" monocytes, whereas basophil activation was induced by T-cell-derived IL3.

66 activity for IgE (IgE-FAB and IgE-BF), (iv) Basophil activation, (v) Cytokines and Chemokines, (vi)

67 as assessed by changes in body temperature, basophil activation, and basophil depletion.

68 tween IgE and FcRI, preventing mast cell and basophil activation, and blocks IgE binding to CD23 on B

69 l symptoms, nasal airflow, nasal secretions, basophil activation, and plasma cytokine levels.

70 rms of patients' IgE-binding capacity, human basophil activation, and positive skin reaction in sensi

71 but not trans-UCA, enhanced the IgE-mediated basophil activation, as well as IgE- and calcium-mediate

72 was characterized by augmented serum-induced basophil activation, increased levels of autoantibodies

73 vo effects of statins on IgE-mediated MC and basophil activation.

74 ross-inhibition of patients' IgE binding and basophil activation.

75 ted ovalbumin displayed lower Ig-binding and basophil-activation capacities for sera from both allerg

76 B cell, platelet, neutrophil, and mast cell/basophil activity.

77 esis and release of sulfidoleukotrienes from basophils after activation with different stimuli, by Fc

78 For pistachio, the degranulation of basophils after challenge with the harshest heat/pressur

79 xpression of CD63 and CD203c was observed on basophils after pre-incubation with histamine or the spe

80 The ratio of the percentage of CD63(+) basophils after stimulation with peanut and after stimul

81 epsilon receptor signaling in mast cells and basophils, all of which have been implicated in the path

82 CDsens, a measure of basophil allergen sensitivity, was significantly higher

83 ciated with severity (P = .001), whereas the basophil allergen threshold sensitivity CD-sens (1/EC(5)

84 Modules of mast cell/basophil and neutrophil function show temporally stable

85 A combination of baseline basophil and serologic biomarkers defined a subset of pa

86 Btk activity is regulated differentially in basophils and B cells.

87 CE I, and AP-N were able to activate patient basophils and elicit positive responses in skin prick te

88 gE receptors (FcepsilonRI) on mast cells and basophils and low-affinity IgE receptors (FcepsilonRII)

89 to its high-affinity receptor FcepsilonRI on basophils and mast cells is a central event in the devel

90 E levels and causes FcepsilonRI receptors on basophils and mast cells to be downregulated.

91 We conclude that IL-33 isoforms activate basophils and mast cells to drive type 2 inflammation in

92 munohistochemistry, we found that numbers of basophils and mast cells were markedly increased in the

93 y and RNA sequencing of sputum cells suggest basophils and mast cells, not ILC2s, are the cellular so

94 ng compounds were more potent for inhibiting basophils and mast cells.

95 o facilitate degranulation of mast cells and basophils and promote TH2 immunity, mechanisms that are

96 R regulates IgE-dependent processes in human basophils and provides a novel function of the H4R preve

97 e surface IgE and FcepsilonRI level on human basophils and the human FcepsilonRIalpha transgenic mous

98 e, the interaction between human B cells and basophils and the mechanism underlying this interaction

99 counts (neutrophils, monocytes, lymphocytes, basophils, and eosinophils) in a multi-ancestry discover

100 pathogenic mechanism, including mast cells, basophils, and eosinophils.

101 There is evidence that mast cells, basophils, and IgE can contribute to immune responses to

102 ex crosslinking of FcgammaRs on macrophages, basophils, and neutrophils; anaphylaxis mediated by bind

103 3a and C5a to their receptors on mast cells, basophils, and other myeloid cells; and direct activatio

104 Basophil anergy thus seems to function as activation bar

105 Inactivation of basophils ('basophil anergy') was observed in about 10% of the cohor

106 Human basophils are a source of -and are activated by - IL-31

107 Mast cells and basophils are developmentally related cells whose activa

108 Basophils are important effector cells involved in the p

109 So far the effects of IL-31 on human basophils are unknown.

110 ferent clinical characteristics, pointing to basophils as important players in CU pathophysiology.

111 rtant in eosinophilic asthma and that sputum basophil assessment could be a useful additional indicat

112 Mast cell (MC)- and basophil-associated inflammatory diseases are a consider

113 and the effector functions of mast cells and basophils at mucosal and cutaneous sites of environmenta

114 Inactivation of basophils ('basophil anergy') was observed in about 10%

115 ting an important novel function of IL-31 in basophil biology.

116 To analyse the functional role of IL-31 in basophil biology.

117 w-derived mast cells and bone marrow-derived basophils (BMBs) at rest, upon an adaptive-type activati

118 ation +/- omalizumab showed a dependency for basophil-bound IgE, substantiated by a requirement for c

119 Conversely, basophils (but not mast cells) released histamine and ma

120 nique IgE-binding crystallin fold, activates basophils by a novel, cross-linking-independent mechanis

121 SEEs can activate basophils by simultaneously binding as antigens in the c

122 nstrated that both activated human and mouse basophils can form extracellular DNA traps (BETs) contai

123 of an apparent lack of phagocytic activity, basophils can kill bacteria through BET formation.

124 equate for quantification of upregulation of basophil CD203c and identification of a population of CD

125 BATs to measure upregulation of basophil CD203c and induction of a CD63(hi) basophil pop

126 E or IL-3 resulted in strong upregulation of basophil CD203c in samples collected in EDTA or heparin,

127 Immunologic outcomes included measurement of basophil CD63 expression and histamine release and casei

128 Milk-induced basophil CD63 expression was transiently reduced in whol

129 human peanut- and cat-allergic IgE-mediated basophil CD63 induction indicative of anaphylactic degra

130 The percentage of ex vivo-activated basophils (CD63(+)/CCR3(+) cells; after stimulation with

131 ts of these drugs on the growth of the human basophil cell line KU812 and the human mast cell line HM

132 It is thought that mast cells and IgE and basophils (circulating granulocytes, whose functions par

133 Basophils contributed to a lesser extent.

134 Strikingly, basophil contribution and histamine predominance in mice

135 verall, IL-33 augmented IL-13 secretion from basophils cotreated with IL-3, with minimal effects on h

136 ible modulation of B cell differentiation by basophils could point to new therapeutic targets.

137 factor CEBPA The fine-mapped variant at this basophil count association near CEBPA overlapped an enha

138 nonreleasers in HR but not in BAT had lower basophil count compared to releasers (249 vs 630 counts/

139 e of the CEBPE variant associated with lower basophil count has been previously associated with Ameri

140 certain biomarkers (positive BAT result and basophil count) may help to predict the likelihood of re

141 loci, including at a previously undiscovered basophil count-associated locus near the master hematopo

142 We additionally identified basophil count-associated variation at another more plei

143 LC7A7, CEBPA and CRBN-TRNT1) associated with basophil count.

144 ted in a significant decrease in circulating basophil counts compared with those in prechallenge samp

145 he urticaria activity score and with reduced basophil counts.

146 vels increased, while both CD203c+ and CD63+ basophils decreased significantly more over time in the

147 In contrast, presence of basophils decreased the likelihood of resolution (HR, 0.

148 infections with S. venezuelensis Studies in basophil-deficient Mcpt8(DTR) mice revealed a small but

149 the use of PLA2denat decreased IgE-mediated basophil degranulation as compared to the native form of

150 otential of Der p 13 were examined by ELISA, basophil degranulation assays, and in vitro airway epith

151 IgE activities were tested in basophil degranulation assays.

152 s well as the limited propensity to activate basophil degranulation classifies Der p 13 as a minor HD

153 SE-based proliferation assays and ELISA, and basophil degranulation were examined after PLA2denat -MB

154 property, termed "cytokinergic", of inducing basophil degranulation without the intervention of an an

155 ited IgE binding to Cyp c 1, Cyp c 1-induced basophil degranulation, and allergic symptoms caused by

156 IgE recognition of Cyp c 1, Cyp c 1-specific basophil degranulation, and Cyp c 1-induced allergic sym

157 pectedly, each anti-SEE mediated SEE-induced basophil degranulation, and IgG1 or antigen-binding frag

158 s for 60min, led to an important decrease of basophil degranulation.

159 n body temperature, basophil activation, and basophil depletion.

160 echanisms of AIT include early mast cell and basophil desensitization effects, regulation of T- and B

161 ophils, monocytes, lymphocytes, eosinophils, basophils) differ by ethnicity.

162 ecifically regulates CEBPA expression during basophil differentiation.

163 es indicated that in certain individuals the basophils do not respond toward allergens due to a desen

164 eatic cancer confirmed a functional role for basophils during tumor progression.

165 duce PC differentiation, we found that human basophils enhance B cell proliferation, class switching,

166 Intratumoral basophils enhanced CD8(+) T-cell infiltration via produc

167 ells in target organs, including mast cells, basophils, eosinophils, and type 2 innate lymphoid cells

168 type 2 immune cells, such as Th2, ILC2, and basophils exerting their effect by production of IL-4, I

169 We could show that highly purified basophils express the H1R, H2R, and H4R but not the H3R

170 Human basophils expressed higher H4R mRNA levels as compared t

171 Here, we show that basophils expressing IL4 are enriched in tumor-draining

172 ly, there were lower proportions of CRTh2(+) basophils expressing surface CD203c(bright) (all P < .00

173 crease in free IgE levels and an increase in basophil expression of the high-affinity IgE receptor.

174 ous urticaria and was released from isolated basophils following either anti-IgE, IL-3 or fMLP stimul

175 trend (P=0.08) towards a reduction in sputum basophils following increased inhaled corticosteroid (IC

176 ased on an extensive screening with sera and basophils from allergic patients, two hypoallergenic mos

177 1 protein expressions were analysed on human basophils from healthy donors.

178 venoms, pork kidney, and cetuximab activated basophils from patients with IgE to alpha-gal.

179 Basophils from patients with systemic lupus erythematosu

180 Although MCs and basophils from the C57BL/6J mouse strain were responsive

181 t seen in the"NON"responders, suggesting the basophil function was not detected in this subgroup.

182 amine H4 receptor (H4R), in modulating human basophil function.

183 We also suggested that significant basophil functions might be detected among the "LOW"resp

184 esults in the release of mediators stored in basophil granules, such as histamine and proteases, and

185 We sought to investigate whether basophils have an important role in human anaphylaxis.

186 ll, our data demonstrate that mast cells and basophils have cell- and activation-specific transcripti

187 l importance but mast cells, antibodies, and basophils have few or no nonredundant roles.

188 (SHR/T) and low responders in the automated basophil histamine release test (Allerport HRT).

189 Serum inhibitory activity for IgE-FAB and basophil histamine release were also significantly great

190 e capacity to cross-link IgE and degranulate basophils, however, this capacity was diminished when co

191 asability, reversing basopenia and improving basophil IgE receptor function, reducing activity of IgG

192 d allergen-induced histamine liberation from basophils in allergic donors.

193 The role of basophils in anaphylaxis is unclear.

194 PC in vitro, and acted synergistically with basophils in enhancing Ab production.

195 We also assessed a possible contribution of basophils in immune responses to S. venezuelensis By imm

196 Basophils in medium alone or with IL-3 +/- anti-IgE were

197 ibit anti-IgE-induced histamine release from basophils in nonallergic subjects and allergen-induced h

198 responses from eosinophils, mast cells, and basophils in the affected tissues.

199 ngs imply an important and specific role for basophils in the pathophysiology of human anaphylaxis.

200 rFel d 7 activated basophils in vitro and inhibited IgE binding to rCan f 1

201 l-related genes as compared with human blood basophils in whole-transcriptome microarray analyses.

202 gen showed expression of surface antigens on basophils increased in a concentration-dependent manner.

203 After antigenic inflammation, basophils initiate transmigration like other granulocyte

204 epsilonRI complexes activates mast cells and basophils, initiating the allergic response.

205 and regulation of dendritic cell, mast cell, basophil, innate lymphoid cell, T-cell, and B-cell respo

206 E expressed on the surface of mast cells and basophils interacts with antigens, via bound IgE antibod

207 ticularly Th2 T lymphocytes, eosinophils and basophils into nasal mucosal tissue that results in the

208 The recruitment of basophils into TDLN relied partly upon the release of ch

209 d identification of a population of CD63(hi) basophils, irrespective of whether the specimens were an

210 dependent histamine release in ex vivo blood basophils is largely suppressed in a leukemia patient tr

211 duction and the activation of mast cells and basophils, is well understood but the mechanisms related

212 lated from peripheral connective tissues and basophils isolated from spleen and blood.

213 sitized mice were investigated by ELISA, rat basophil leukemia assay, T-cell proliferation experiment

214 g human and mouse mast cells, as well as rat basophil leukemia cells, and in vivo in mice.

215 OAL WBC types (ie, neutrophils, eosinophils, basophils, lymphocytes, and monocytes) in peripheral blo

216 release and the contribution of mast cells, basophils, macrophages, and neutrophils.

217 tively on human eosinophils, mast cells, and basophils, making it an ideal target for the treatment o

218 c allergens upregulate the surface-expressed basophil marker CD203c in sensitized subjects, a respons

219 Like basophils, mast cells express the high-affinity receptor

220 Our findings suggest that basophils may be particularly important in eosinophilic

221 However, previous studies indicate that basophils might be of relevance.

222 Thus, human basophils modulate B cell differentiation into Ab-produc

223 jected with mediator antagonists or in which basophils, monocytes/macrophages, or neutrophils had bee

224 Platelet MPs (3.5-fold; P < .01) and basophil MPs (2.5-fold; P < .05) were increased only in

225 E receptor [FcepsilonRI](+)c-kit(+)MPs), and basophil MPs (CD203c(+)c-kit(-)MPs).

226 the mean fluorescence intensity of CD203c on basophil MPs.

227 Reactivity of CU patients' peripheral blood basophils (n=60) to specific anti-FcepsilonRI and IgE-in

228 es controls the contributions of mast cells, basophils, neutrophils, and macrophages to IgG subclass-

229 Still, BAT could diagnose subjects with low basophil number in contrast to HR.

230 CSU subjects were associated with increased basophil numbers (total leukocyte histamine content) and

231 Sputum basophil numbers are increased in allergic asthmatics, b

232 body temperatures, plasma histamine levels, basophil numbers, antigen-specific IgE, cytokine levels,

233 nt activation and histamine release in blood basophils obtained from allergic patients (n=11) and non

234 nditions whereby IgE-dependent activation of basophils occurs despite the absence of any known allerg

235 hl p 12 induced dose-dependent activation in basophils of these patients.

236 is negative or the patient has non-responder basophils, OFC may still be indicated.

237 IgE-stripped basophils or a mast cell line were used in passive sensi

238 Furthermore, murine basophils or their mediators enhance memory responses an

239 Functionally, mast cells and basophils overlap in their ability to produce several me

240 gonist ST-1006 initiated active migration of basophils (P < 0.001).

241 thmatics, but it is unclear what role airway basophils play in "TH2-low" asthma phenotypes.

242 Our study identifies a critical role basophils play in tumor rejection and that this role can

243 basophil CD203c and induction of a CD63(hi) basophil population can be conducted with blood obtained

244 Basophils present in TDLNs correlated significantly with

245 One subgroup of patients' basophils reacted to FcepsilonRI stimulation, whereas th

246 Spontaneous and allergen-induced basophil reactivity (histamine release, CD63 expression,

247 o three immunologic subgroups based on their basophil reactivity and frequency.

248 with milk OIT led to distinct alterations in basophil reactivity but not T-cell responses.

249 Pre-OIT basophil reactivity positively associated with occurrenc

250 sive desensitization, both clinically and in basophil reactivity, occurs over time.

251 In this article, we show that neonatal basophils readily produce IL-4, a cytokine that proved t

252 Our results show how basophils recruited and activated in TDLNs under the inf

253 romal lymphopoietin [TSLP]) activating human basophils remains controversial.

254 Basophils represent <1% of circulating leukocytes.

255 gs point to a lectin-dependent activation of basophil requiring IgE but independent of allergen or se

256 An almost complete inhibition of the basophil response was seen in all patients treated with

257 s, removal of IgG did not have any effect on basophil response.

258 show that rfhSP-D inhibited allergen-induced basophil responses at a single-cell level and suppressed

259 been produced and changes in the humoral and basophil responses have been analysed.

260 Flow cytometric analysis of basophil responses implied labeling for CD63, CD203c, an

261 Basophil responses to IL-31 were assessed for chemotaxis

262 In the remainder eight patients, no basophil responses were demonstrable.

263 Allergen-induced basophil responsiveness and histamine release were inhib

264 shrimp-specific IgE in serum and measurable basophil secretory responses to rPen a 1 (shrimp tropomy

265 l efficacy is associated with improvement of basophil sensitivity and an increase in allergen-specifi

266 Total and allergen-specific IgE, IgG and basophil sensitivity were measured before and 8 weeks af

267 Basophil supernatants enhanced the expression of the B c

268 It was associated with a downregulation of basophil Syk and an apparent reduction in the incidence

269 Basophil, T-cell, and dendritic cell activation increase

270 The basophil-targeting effect of ibrutinib was confirmed by

271 The basophil tests' diagnostic performances were not signifi

272 Allergy can be diagnosed using basophil tests.

273 a expression is restricted to mast cells and basophils, this approach would selectively target these

274 ng autoantigens, activation and migration of basophils to lymph nodes, and, as observed most recently

275 mmune responses by triggering mast cells and basophils to release histamine and type 2 helper cytokin

276 aled a small but significant contribution of basophils to S. venezuelensis egg clearance in primary i

277 The derivation of mast-cell and basophil transcriptional signatures underscores their di

278 s (n = 26) in RAST-based assays and with rat basophils transfected with the human FcepsilonRI, respec

279 line and 24 hours after challenge to measure basophil TSLPR expression.

280 nscriptional heterogeneity of mast cells and basophils upon their activation, we performed large-scal

281 e hypothesize that ECs can directly activate basophils via cell-to-cell interaction.

282 Stimulating CU patients' basophils via FcepsilonRI, we identified three distinct

283 The IgE cross-linking capacity of Pin p 1 on basophils was also demonstrated.

284 Migration of basophils was assessed using the modified Boyden chamber

285 However, a CD63(hi) population of basophils was not observed in any conditions in EDTA-tre

286 The number of circulating basophils was significantly lower during anaphylaxis (me

287 vels of CD63 higher than 1.8% and absence of basophils were associated with earlier disease resolutio

288 metric method; passive HR-IgE-stripped donor basophils were incubated with participants' serum and hi

289 Basophils were isolated from the peripheral blood of pat

290 xpressed on TH2 lymphocytes (CRTh2)-positive basophils were measured by means of flow cytometry.

291 on released MPs, mast cells, platelets, and basophils were more highly activated in patients with AE

292 In asthmatics, basophils were positively correlated with sputum eosinop

293 Type 2 innate lymphoid cells and basophils were scarce in BAL fluid.

294 Using flow cytometry, we found that basophils were significantly increased in all asthmatics

295 gulation of CD13, CD63, CD164, and CD203c on basophils, whereas AVL-292 and CNX-774 showed no signifi

296 to FcepsilonRIalpha on dermal mast cells and basophils, which on activation release mediators respons

297 rved minimal homology between mast cells and basophils, which shared more overlap with other circulat

298 CD203c); the absolute number of circulating basophils; whole-blood FCER1A, carboxypeptidase A3 (CPA3

299 y to correlate the functional state of their basophils with the clinical state as well as the composi

300 Degranulation of mast cells and basophils, with release of agents of the allergic respon