ライフサイエンスコーパス: be cloned

1 ter (SGLT) was the first transporter ever to be cloned.

2 04, only 12 years after its molecular target was cloned.

3 encoding amorpha-4,11-diene 12-hydroxylase, was cloned.

4 ll-length cDNA of the 14,538-nt viral genome was cloned.

5 involved in the O-glucosylation pathway have been cloned.

6 volved in endocytotic iron delivery has also been cloned.

7 r genes that control tomato fruit shape have been cloned.

8 e been identified but the genes have not yet been cloned.

9 i have been mapped, no resistance genes have been cloned.

10 e many mu opioid receptor subtypes that have been cloned.

11 while 96 (15%) of the GTs coded by rice have been cloned.

12 ta) from small abalone Haliotis diversicolor were cloned.

13 , Solanum lycopersicum (tomato) SlSERK genes were cloned.

14 d seven antagonistic mAbs to canine (ca)CD28 were cloned.

15 nfected individuals from whom these variants were cloned.

16 These constructs were cloned 1 bp out of frame of EGFP, allowing a -1 bp

17 ted plasmids in which exons 3 or 10 of ACVR2 were cloned +1 bp out of frame of enhanced green fluores

18 All known vernalization genes are cloned according to qualitative variation in vernali

19 f hematopoietic stem/progenitor cells GATA-2 was cloned almost 20 years ago, and elegant genetic anal

20 recover a fragment of interest, PCR products are cloned and sequenced.

21 I reported in PNAS that individual genes can be cloned and isolated by enzymatically cleaving DNA mol

22 allergen Bet v 1 was the first such gene to be cloned and its product characterized.

23 Cells containing the replicon could be cloned and passaged many times in the absence of anti

24 oside phosphorylase (designated PNPHyor) has been cloned and characterized for the first time.

25 In both diseases, the autoantigens have been cloned and characterized; pemphigus antigens are de

26 ochromes c(4) and c(5) from V. cholerae have been cloned and expressed heterologously in Escherichia

27 Cytochrome aa(3)-600 has been cloned and expressed in a His-tagged form in B. sub

28 The carboxylesterase Est55 has been cloned and expressed in Bacillus subtilis strains.

29 eir formation, but not in diploids that have been cloned and frozen.

30 Thus far, CD80 and CD86 have been cloned and functionally characterized only in mamma

31 channels, but only a fraction of these have been cloned and functionally tested.

32 gene encoding the 53-kDa enzyme subunit has been cloned and matched to the enzyme subunit by mass sp

33 in Xanthobacter autotrophicus strain Py2 has been cloned and overexpressed, allowing the first detail

34 dinomine (gdn) biosynthetic gene cluster has been cloned and sequenced and includes 26 open reading f

35 ly of 11 amino acid peptide antibiotics, has been cloned and sequenced from Streptomyces viridochromo

36 Streptomyces griseochromogenes, has recently been cloned and sequenced.

37 -phosphate O-acyltransferases (AGPATs), have been cloned and studied.

38 lypeptide (Ntcp) from the respective species was cloned and analyzed for HBV and HDV receptor activit

39 this study, a full-length cDNA encoding CPR was cloned and characterized from T. cinnabarinus (desig

40 A beta-carbonic anhydrase (CA, EC 4.2.1.1) was cloned and characterized from this organism, denomin

41 Two decades later, it was cloned and characterized to be a transmembrane mucin

42 Human SPR was cloned and characterized.

43 dcd1 was cloned and encodes a putative B'' regulatory subunit

44 of HEV, the region (amino acids 960 to 1204) was cloned and expressed as histidine-tagged protein in

45 expressed by the homologous parasite isolate was cloned and expressed as recombinant protein.

46 re diffocin genetic locus of more than 20 kb was cloned and expressed in Bacillus subtilis, and this

47 This enzyme was cloned and expressed in E. coli, and mass spectrosco

48 Here, the tth111IIRM gene was cloned and expressed in E. coli, and Tth111II was pu

49 erthermophilic archaeron Sulfolobus tokodaii was cloned and expressed in E. coli.

50 ferase from Narcissus sp. and Galanthus spp. was cloned and expressed in Escherichia coli Biochemical

51 og in Methanocaldococcus jannaschii (MJ1391) was cloned and expressed in Escherichia coli, and the pr

52 Therefore, this gene was cloned and expressed in Escherichia coli, and the re

53 A helicase from Staphylococcus aureus USA300 was cloned and expressed in Escherichia coli.

54 nthase (DEBS TE) appended to the C-terminus, was cloned and expressed in Escherichia coli.

55 th redundant biological functions, each gene was cloned and expressed in Escherichia coli.

56 The bfrB gene from Pseudomonas aeruginosa was cloned and expressed in Escherichia coli.

57 Human MATN-1 was cloned and expressed in Pichia pastoris and purified

58 p-regulated at different moisture gradients, was cloned and expressed in tobacco.

59 A cDNA encoding this protein was cloned and expressed in vitro, and its recognition b

60 racellular and neck domain of human langerin was cloned and expressed to produce a recombinant active

61 ; Clan SB, family S8) of Leishmania donovani was cloned and found to possess a unique catalytic triad

62 target protein for DMI fungicides (VvCYP51) was cloned and investigated.

63 The MJ1179 gene was cloned and its protein product heterologously expres

64 This gene was cloned and over-expressed in Escherichia coli BL21(D

65 ps of lindane (gamma-HCH) biotransformation, was cloned and overexpressed in Escherichia coli .

66 The recombinant allergen was cloned and purified, showing similar IgE reactivity

67 Its promoter gm-hir1 was cloned and revealed to strongly express a fluorescen

68 yles from genetically diverse almond samples was cloned and sequenced and then analyzed for changes a

69 Its cDNA was cloned and sequenced by RT-PCR and nested PCR using

70 cluster, comprising 29 open reading frames, was cloned and sequenced, and shown to possess a type II

71 The 5'-flanking region (5 kb) of human RPGR was cloned and sequenced.

72 The cDNA of the mature trypsin was cloned and sequenced.

73 is metabolic regulation, the Leishmania GMPR was cloned and shown to be sufficient to complement the

74 The gene was cloned and the sequence was codon optimized and expr

75 igrant/transitional and mature naive B cells were cloned and assessed for their ability to bind self-

76 s including six alpha and five beta subunits were cloned and characterized from silkworm.

77 eam promoter sequences of soybean SMT2 genes were cloned and characterized.

78 Two candidate miRNAs, miR-377 and miR-217, were cloned and co-transfected with a luciferase vector

79 Pakistani patients infected with subtype 3a were cloned and compared with other subtype 3a sequences

80 uman, rat, frog, insect and plant homologues were cloned and Escherichia coli-recombinant proteins ca

81 Variable regions were cloned and expressed as recombinant IgG(4).

82 These two receptor genes along with CsPYL1 were cloned and expressed in a heterologous system.

83 The TbPMM and TbPAGM genes were cloned and expressed in Escherichia coli and the Tb

84 anine Gal-1, -2, -3, -4, -7, -8, -9, and -12 were cloned and expressed in Escherichia coli as GST fus

85 * The switchgrass PvMYB4 cDNAs were cloned and expressed in Escherichia coli, yeast, to

86 a1, beta2, beta3, and beta4) subunits of CK2 were cloned and expressed in Escherichia coli.

87 talliredigens and Corynebacterium glutamicum were cloned and expressed in Escherichia coli.

88 DP-glucose 4-epimerase genes of B. anthracis were cloned and expressed in Escherichia coli.

89 CFTR wild-type and p.R75Q were cloned and expressed in HEK293 cells, and relative

90 re previously not known to interact with ATP were cloned and expressed to validate the result.

91 The genes encoding four diverse candidates were cloned and expressed, and the enzymes were purified

92 The genes were cloned and expressed, but none of them oxidized 18:

93 ogs to other bacterial transcription factors were cloned and introduced into E. coli carrying a p66 p

94 housekeeping genes selected from C. maculata were cloned and investigated.

95 In this research, barley and wheat DME genes were cloned and localized on the syntenous chromosomes.

96 lent species D adenoviruses Ad26, 28, and 48 were cloned and modified to express the influenza virus

97 * To address these questions TGA1-like genes were cloned and sequenced from a number of grasses and a

98 Porcine BHMT and BHMT-2 cDNAs were cloned and sequenced, and their 5' and 3' UTR were

99 Positive samples were cloned and sequenced.

100 and E. coli-inducible Arabidopsis promoters were cloned and tested in transient expression assays in

101 phytaspase genes were identified, the cDNAs were cloned and the recombinant enzymes were obtained af

102 promoters of the mouse and human CD36 genes were cloned and their regulation by AhR was analyzed.

103 Three mutations (P1400S, S2195F, and L717V) were cloned and transfected into a mammalian cell line f

104 enes derived from a cDNA microarray analysis were cloned and transiently overexpressed to evaluate th

105 hermovirga lienii and Archaeoglobus fulgidus were cloned and used to generate standards for bacterial

106 The genes were cloned and, except for cop5, functionally expressed

107 Many of these genes have been cloned, and disruptions of their functions are asso

108 The gene for this enzyme has been cloned, and the corresponding protein has been over

109 To test this hypothesis, CBO0515 has been cloned, and the encoded polypeptide was purified an

110 The gene of Platypodium elegans lectin A has been cloned, and the resulting 261-amino acid protein be

111 use genomic DNA containing the Cnn2 promoter was cloned, and a nested set of 5' truncations was studi

112 mately 1.5-kb mouse Samd9L promoter fragment was cloned, and a series of 5' deletion constructs were

113 The major Siberian hamster allergen was cloned, and allergenic properties were characterized

114 th the typical stacked-brick binding pattern was cloned, and deletion of the cluster was performed.

115 This novel cytokine was cloned, and its expression was analyzed using recomb

116 e transcription start site) of the JDP2 gene was cloned, and promoter activity was analyzed.

117 The gapdh gene was cloned, and recombinant GAPDH (rGAPDH) was expressed

118 ddition, a putative transformer2 gene (tra2) was cloned, and the structural feature and expression pr

119 MtSGR sequence, an alfalfa SGR gene (MsSGR) was cloned, and transgenic alfalfa lines were produced b

120 Chondrichthyes (catshark and elephant shark) were cloned, and it was found that pineal glands and ret

121 genes from Chloroflexus aurantiacus J-10-fl were cloned, and the corresponding proteins were express

122 ubtype A, as well as subtypes A/D, C, and D, were cloned, and their neutralization profiles were char

123 ymes in the biosynthesis pathway of melanin, were cloned, and their temporal expression patterns in t

124 These variants were cloned, and then expressed using the magnICON trans

125 The strain C500 has been cloned as an infectious, pathogenic bacterial artif

126 .338 is expressed independently of PCBP2 and was cloned as a 590-bp RNA gene, termed TUC338.

127 The cell fate determination factor Dachshund was cloned as a dominant inhibitor of the hyperactive ep

128 Fas apoptosis inhibitory molecule (FAIM) was cloned as a mediator of Fas resistance that is highl

129 Here, FEN1 was cloned as a suppressor of transcriptional gene silen

130 The genes expressing these proteins were cloned as an operon transcribed from P(trc) into is

131 ts value as a resource, targeted transcripts were cloned as candidates for nearly all of the structur

132 ndmA, ndmB, and ndmD were cloned as His(6) fusion genes, expressed in Escheri

133 bodies with "matched" heavy and light chains were cloned as IgG1, and those of high affinity for spec

134 entire asukamycin biosynthetic gene cluster was cloned, assembled, and expressed heterologously in S

135 A His6 tag was cloned at the N terminus, along with R403Q, to facil

136 ene segments on mature peptide part of MAF-1 were cloned, based on the primers designed according to

137 A second C5a receptor, C5L2, has also been cloned but has received much less attention because

138 Some genes cannot be cloned by conventional methods because in most cases

139 de ion channels and transporters that cannot be cloned by conventional techniques requiring E. coli.

140 pacX was cloned by impala transposon mutagenesis.

141 The TLA2 gene, causing the tla2 phenotype, was cloned by mapping the insertion site and upon comple

142 Emb14 was cloned by transposon tagging and was confirmed by an

143 Seven novel AZIN1 splice variants ("SV2-8") were cloned by polymerase chain reaction from the LX2 hu

144 m the fungal pathogen Malassezia globosa has been cloned, characterized, and studied for its inhibiti

145 nductance (MscL and MscS, respectively) have been cloned, crystallized, and subjected to biophysical

146 in the plasma of the animal from which they were cloned, demonstrating the power of mAb isolation fo

147 Bait-prey junctions are cloned directly from isolated genomic DNA using LAM-

148 porter plasmid where bim-3'UTR mRNA sequence was cloned downstream of a luciferase gene.

149 wherein the Bcr 3' untranslated region (UTR) was cloned downstream of a luciferase reporter showed re

150 Wild-type and mutant MMP1 cDNAs were cloned downstream of a glucocorticoid response elem

151 ed adenylate/uridylate-rich elements (AREs), were cloned downstream of a reporter gene.

152 PO), its receptor (EPOR), and janus kinase 2 were cloned; established to be essential for definitive

153 Each protein has been cloned, expressed, and purified from Escherichia co

154 In this study, Stt7 kinase has been cloned, expressed, and purified in a heterologous h

155 tobacter sp. NAS-14.1, designated sCoaT, has been cloned, expressed, and purified.

156 region of the gene encoding these structures was cloned, expressed in vivo, and found to strongly inh

157 N-terminal mitochondrial targeting sequence, was cloned, expressed, and characterized as a 65-kDa acy

158 e Acidothermus cellulolyticus P(1B-5)-ATPase was cloned, expressed, and purified (P(1B-5)-Hr).

159 lase superfamily in Deinococcus radiodurans, was cloned, expressed, and purified to homogeneity.

160 Recombinant Kindlin-3 was cloned, expressed, and purified, and its domain orga

161 The enolase was cloned, expressed, purified, and used to generate ra

162 dohydrolase superfamily belonging to cog1228 were cloned, expressed, and purified to homogeneity.

163 om the amidohydrolase superfamily of enzymes were cloned, expressed, and purified to homogeneity.

164 Recently, a novel gene was cloned for autosomal recessive retinitis pigmentosa

165 moter, transcription start site, and the TRE were cloned for functional analysis.

166 omplex (MHC) because the specificity domains are cloned from the variable chains of a CD19 monoclonal

167 s demonstrate that TALE-specific R genes can be cloned from large-genome crops with a highly efficien

168 a phylum, and although alpha-like OctRs have been cloned from Balanus improvisus (BiOctR) and Drosoph

169 Although a receptor for A2t has been cloned from bone marrow stromal cells, data contain

170 , the BOLTING time control 1 (BTC1) gene has been cloned from this locus.

171 roximately 91% sequence identity to Bet v 7) was cloned from a cDNA library and expressed in Escheric

172 -acetylserotonin-O-methyltransferase (ASMT), was cloned from apple rootstock, Malus zumi.

173 the serine/arginine (SR)-rich protein family was cloned from Arachis diogoi, a wild relative of peanu

174 ore, a panel of human recombinant antibodies was cloned from different B cell subpopulations, and the

175 The extracelluar domain of canine (ca)CD28 was cloned from dog peripheral blood mononuclear cells.

176 s in vitro study, feline erythropoietin cDNA was cloned from feline renal tissue and utilized in the

177 AGXT2 was cloned from human kidney cDNA and overexpressed in C

178 urportedly encoding for acetylcholinesterase was cloned from maize.

179 The Muc5ac cDNA was cloned from mouse lungs and tagged internally with G

180 D-xylulose-5-phosphate synthase (DXS) enzyme was cloned from Populus trichocarpa, and the recombinant

181 ing frame of 327 bp encoding 109 amino acids was cloned from rice bean seeds using degenerate primer

182 CD300d cDNA was cloned from RNA obtained from human peripheral blood

183 CTOMT1 was cloned from Solanum lycopersicum cv. M82 and express

184 A type 2 metallothionein gene, SsMT2, was cloned from Suaeda salsa, a salt- and alkali-toleran

185 The HCV1406 TCR was cloned from T cells that expanded when a hepatitis C

186 A TcNr cDNA encoding a putative Nr was cloned from Taiwanofungus camphorata.

187 The Cry3Aa receptor cadherin was cloned from Tenebrio molitor larval midgut mRNA, and

188 CDPK1 was cloned from the genome of Cryptosporidium parvum, an

189 cently, a PKM isoform, known as PKM Apl III, was cloned from the nervous system of Aplysia.

190 id carboxyl extension protein (CEP12) domain was cloned from the potato cyst nematode Globodera rosto

191 t nematode (Heterodera glycines), 10A06 gene was cloned from the sugar beet cyst nematode (Heterodera

192 SlCAT2 was cloned from tomato flower cDNA, over-produced in Esc

193 * ACR3 was cloned from yeast and transformed into wild-type and

194 Drug-specific T cells were cloned from blood and inflamed skin, and cellular p

195 synthase (QNS) and acridone synthase (ACS), were cloned from Citrus microcarpa (Rutaceae).

196 eral nsLTPs-encoding cDNA and gene sequences were cloned from Coffea arabica and Coffea canephora spe

197 apacity to grow under restrictive conditions were cloned from cultured Deltahgprt/Deltaxprt parasites

198 Orthologs of OR7D4 were cloned from different primate species.

199 cDNAs encoding MdBIS1 to MdBIS4 were cloned from fire-blight-infected shoots of apple 'H

200 essary for herpes simplex virus (HSV) fusion were cloned from herpes B virus (BV) (or macacine herpes

201 For these studies, libraries of Env proteins were cloned from infected subjects and screened for infe

202 PHD and FIH were cloned from mouse embryonic stem cells.

203 Their complete amino acid sequences were cloned from our L. variegatus cDNA library.

204 71AN24, CYP71AP13, CYP71AU50, and CYP736A117 were cloned from P. mume 'Nanko' using publicly availabl

205 Fertile mice were cloned from several neurons, establishing the compa

206 Matched heavy- and light-chain sequences were cloned from single IgG(4)(+) B cells and expressed

207 Vps2B, Vps20, Vps24, Snf7, Vps46, and Vps60) were cloned from Spodoptera frugiperda Using a viral com

208 Two unique SPHK cDNAs were cloned from the annotated At4g21540 locus of Arabid

209 The two genes were cloned from the chromosome of Pseudomonas aeruginos

210 essary for herpes simplex virus (HSV) fusion were cloned from the saimiriine herpesvirus 1 (SaHV-1) g

211 mologs of groups 1 to 4 sulfate transporters were cloned from these Astragalus species to investigate

212 ethyltransferase genes, CmarsM7 and CmarsM8, were cloned from this organism and demonstrated to confe

213 Two families of sncRNAs were cloned from this region, and each family was predic

214 IPK genes were cloned from two organisms identified in the search,

215 MYB80 homologs were cloned from wheat, rice, canola and cotton.

216 The sigma-2 receptor, whose gene remains to be cloned, has been validated as a biomarker for tumour

217 iochemical validation, a full-length ZmTps21 was cloned, heterologously expressed in Escherichia coli

218 represent the first insertional phenotype to be cloned in amphibians.

219 meric G protein-coupled receptors (GPCRs) to be cloned in any organism.

220 covery that even genes from animal cells can be cloned in bacteria.

221 generating stable cell lines the sgRNAs have been cloned in a lentivirus backbone containing PiggyBac

222 redicted unique signaling capabilities, have been cloned in fetal brain tissue.

223 receptor type 2 (CB2) is a class A GPCR that was cloned in 1993 while looking for an alternative rece

224 ene responsible for the mutant wax phenotype was cloned in a forward genetic screen and identified to

225 Consequently, the vgrG1 gene was cloned in pBI-EGFP and pET-30a vectors to be express

226 get SAMDC gene fragments of three homologues were cloned in a hairpin RNA construct under the control

227 the catalytic domains of Cdc25A, -B, and -C were cloned individually into a prokaryotic expression v

228 Genomes can be cloned; individuals cannot.

229 hallmark of the 4280 TagModules is that they are cloned into a Gateway entry vector, thus facilitatin

230 The scaffolds and proteins are cloned into inducible plasmids and expressed to form

231 regulatory elements and unique sequence tags are cloned into plasmids to generate a library of report

232 ncodes a novel monofunctional PPCS which has been cloned into pET23a and expressed in Escherichia col

233 ulent virus strain, the HSV-1(McKrae) genome was cloned into a bacterial artificial chromosome plasmi

234 The full-length cDNA was cloned into a baculovirus expression vector, and rec

235 A human MMP3 promoter was cloned into a luciferase reporter plasmid, pMMP3, an

236 The MHC-I cDNA was cloned into a mammalian expression vector, and stabl

237 C1 domain from the collagen alpha3(IV) chain was cloned into a mammalian expression vector, pCI-neo,

238 mecA homolog free of its regulatory elements was cloned into a plasmid and introduced into the backgr

239 Its gene was cloned into an expression vector with an N-terminal

240 PqqE from K. pneumoniae was cloned into Escherichia coli and expressed as the na

241 ide (LPS) and OmpX in serum resistance, ompX was cloned into Escherichia coli D21 and three isogenic

242 The entire mre operon was cloned into Escherichia coli JM109 and the transform

243 Human gammaS-crystallin cDNA was cloned into pET-20b(+), and the G18V mutant was gene

244 The oriT from an IncP plasmid was cloned into pGNS-BAC to enable conjugal transfer, th

245 rom the protease gene, cathepsin S (Rs-cps), was cloned into the binary vector pFGC5941 in the forwar

246 g cells, a sequence encoding Cre recombinase was cloned into the Il22 locus, and IL22(Cre) mice were

247 ; hTGFbeta2(226/228)) TGFbeta2-encoding cDNA was cloned into the pac.Ad5.CMV.K-N.pA shuttle vector fo

248 full-length open reading frame of human BCO1 was cloned into the pET-28b expression vector with a C-t

249 A 1.4-kb sequence of the A(2B)AR promoter was cloned into the pFRL7 luciferase vector.

250 r the F11 phenotype, the wild-type rquA gene was cloned into the pRK404E1 vector and conjugated into

251 se 368-376 peptide in the context of HLA-A2, were cloned into a gamma-retroviral vector.

252 uences that adopted a G-quadruplex structure were cloned into a luciferase dual vector and examined f

253 T cell receptor (TCR) alpha- and beta-chains were cloned into a retroviral vector.

254 es and other genes involved in RNA silencing were cloned into a vector under an estrogen-inducible pr

255 hetic codon-optimized NS5A genotype 1b genes were cloned into eukaryotic expression plasmids, and the

256 V59I, L97T, I98P, Q99V, and P100N mutations were cloned into FIV Gag-Pol, and those constructs that

257 m passage control and MVC-resistant cultures were cloned into NL4-3 via yeast-based recombination fol

258 t lengths corresponding to the CHRNB4 3'-UTR were cloned into pGL3-promoter luciferase reporter vecto

259 and the resulting chimeric mouse-human genes were cloned into plant expression vectors for stable nuc

260 Various regions of the HABP2 promoter region were cloned into reporter constructs, and the promoter a

261 ation of the process, and the shuffled genes were cloned into the backbone of a DNA-launched PRRSV in

262 last 9 and 36 residues from the C-terminus, were cloned into the recombinant expression vector pET-2

263 These genes were cloned into three expression vectors as native sequ

264 TRPM2, the second subfamily member to be cloned, is expressed in many tissues including brain,

265 Although many legumains have been cloned, knowledge about their detailed characterist

266 Full-length functional env genes were cloned longitudinally from these subjects from mont

267 tina, five distinct EAAT-encoding genes have been cloned, making the amphibian retina an excellent sy

268 Four HCN subunits have been cloned, of which HCN1 and HCN2 subunits are predomi

269 0s, but the first member of the family, Hv1, was cloned only recently.

270 Once the proper expression vectors are cloned, our protocol should allow the production of

271 mophilic archaeon Archaeoglobus fulgidus has been cloned, over-expressed in Escherichia coli and bioc

272 M. tuberculosis DapE (MtDapE) was cloned, over-expressed and purified as an N-terminal

273 KEY MESSAGE: The vacuolar SlCAT2 was cloned, over-produced in E. coli and reconstituted i

274 aracterize this activity, the aminopeptidase was cloned, overexpressed, and purified.

275 Consequently, both enzymes were cloned, overexpressed, and purified as recombinant

276 photoferrotroph Rhodobacter ferrooxidans SW2 was cloned, purified, and characterized for the first ti

277 ction of the SAMHD1 protein, the SAMHD1 gene was cloned, recombinant protein was produced, and the ca

278 Their genes have been cloned, sequenced and overexpressed.

279 is the only eukaryotic thiaminase I to have been cloned, sequenced, and expressed.

280 DNA of the protein was cloned, sequenced, and expressed, confirming its ide

281 Six X/Y gene pairs were cloned, sequenced and analyzed from three dioecious

282 gene neighborhoods from 25 of these isolates were cloned, sequenced, and compared with those found in

283 and the previously identified 32 aa peptide was cloned successfully.

284 The gene was cloned, the protein was overexpressed in Escherichia

285 Two homologs of the TVA receptor have been cloned: the original quail TVA receptor, which has

286 In this work, the PHR1 gene of Chlamydomonas was cloned through molecular mapping and shown to encode

287 odel and crop species, and although none has been cloned to date, transcript profiling experiments ha

288 The selected plants were cloned to reduce genetic variability within each ge

289 When trfA genes were cloned under a constitutive promoter in the chromos

290 ent promoter (PompA) identified in this work was cloned upstream of genes encoding fluorescent protei

291 Identified putative promoters were cloned upstream of GFP.

292 ese important genes in vivo, their promoters were cloned upstream of the luxCDABE operon, and lucifer

293 Domestic animals can be cloned using techniques such as embryo splitting and

294 ization requirement duration in winter wheat was cloned using a BC(1)F(2:3) population that segregate

295 hPNPase(old-35) was cloned using an innovative 'overlapping pathway scre

296 Mutants corresponding to a third group were cloned using mapped-based approaches and found to e

297 Human cPLA(2)alpha gene was cloned via RT-PCR followed by site-directed mutagene

298 gment of the 5'-flanking region of this gene was cloned which exhibited robust promoter activity upon

299 3 (88%) of CAZy defined Arabidopsis GTs have been cloned, while 96 (15%) of the GTs coded by rice hav

300 -infected animal from which the env variants were cloned, while clonal and sequential exposure led to