ライフサイエンスコーパス: be purified by

1 ng its solubility in organic solvents, ImmE1 was purified by 1-butanol extraction of isolated membran

2 In most cases, the amide products can be purified by a simple filtration procedure using comme

3 The His-tagged enzyme is purified by a combination of Ni-NTA and orange A agar

4 The glycosphingolipid was purified by a combination of high-performance liquid

5 ) was formed even by 8 M 2-pic, if the 2-pic was purified by a novel Co(III)-affinity distillation pr

6 MHC was purified by a preparative continuous elution gel ele

7 This Mg2+-dependent endonuclease was purified by a series of chromatographic procedures.

8 Overexpressed SHI was purified by a single affinity chromatography step us

9 llulans NRRL Y-2311-1 produced on wheat bran was purified by a single-step chromatographic procedure.

10 mbinant soluble form in Escherichia coli and was purified by a two-step procedure to near-homogeneity

11 -dependent 1,2-PD degradation by S. enterica were purified by a combination of detergent extraction a

12 Spores were purified by a combination of isopycnic Percoll grad

13 ECoG signals were purified by a denoising procedure of wavelet decomp

14 copGFP(+) ICC from the jejunum were purified by a fluorescence-activated cell sorter an

15 Bound cytoplasmic proteins were purified by a photocleavable biotin tag and stretch

16 The resultant peptides were purified by a two-dimensional method: size exclusio

17 in-CBD fusion, and the stable fusion protein is purified by adsorption onto a chitin column.

18 via the target binding site, the complex can be purified by affinity capture via the peptide tag afte

19 more avidly to phosphoethanolamine and could be purified by affinity chromatography using phosphoetha

20 It has been purified by affinity chromatography and has a molec

21 ntigen recognized by the inhibitory antibody was purified by affinity chromatography and identified b

22 The latter form of the enzyme was purified by affinity chromatography and shown to hav

23 quently, protein produced in mammalian cells was purified by affinity chromatography and used to immu

24 The recombinant human Hsp72 was purified by affinity chromatography from insect cell

25 The protein was purified by affinity chromatography on an anti-SOD a

26 The expressed enzyme was purified by affinity chromatography on Ni(2+)-agaros

27 eEF-1 was purified by affinity chromatography on tRNA-Sepharos

28 The FCBP was purified by affinity chromatography using FC-linked

29 CPO was purified by affinity chromatography, and the purifie

30 P47 was purified by affinity chromatography, digested with e

31 e active fraction of the labeled preparation was purified by affinity chromatography.

32 A principal trophozoite cysteine protease was purified by affinity chromatography.

33 gestion with phage endolysin, InlA-MH(6)-Cws was purified by affinity chromatography.

34 Recombinant P69 produced in insect cells was purified by affinity chromatography.

35 cellulosome produced by the S mutant strain was purified by affinity digestion, characterized enzyma

36 Expressed enzymes were purified by affinity chromatography and analyzed by

37 g and cleavage-secretion, ACE-bound proteins were purified by affinity chromatography and characteriz

38 tic peptides from the photolabeled terminase were purified by affinity chromatography and reverse-pha

39 BDE) secreted by Yersinia pseudotuberculosis were purified by affinity chromatography and used as imm

40 immunoprecipitation with anti-SMN antibodies were purified by affinity chromatography from extracts o

41 Wild-type (wt) and all mutant CRPs were purified by affinity chromatography on PCh-, pneumo

42 Recombinant and native RPE65 were purified by affinity chromatography.

43 ing complexes into structural domains, which were purified by affinity chromatography.

44 scherichia coli and the recombinant proteins were purified by affinity chromatography.

45 a and lambda fractions of anti-6B antibodies were purified by affinity chromatography.

46 DEK autoantibodies and immune complexes were purified by affinity column chromatography and anal

47 Recombinant non-lipidated proteins were purified by affinity or ion exchange chromatography

48 DEK autoantibodies and ICs were purified by affinity-column chromatography and anal

49 he culture media of transfected HEK293 cells was purified by ammonium sulfate fractionation and nicke

50 tide hydroxamate-sensitive shedding activity was purified by ammonium sulfate precipitation, DEAE chr

51 ely 40 microM) and reduced when mitochondria are purified by an isoosmotic density-gradient method.

52 The stocks can be purified by an iodixanol (OptiPrep) gradient centrifu

53 th produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromat

54 While a portion of each polysaccharide can be purified by anion-exchange chromatography, the side g

55 ell, and testis extracts from which SBP2 has been purified by anion exchange and RNA affinity chromat

56 al transglutaminase of Physarum polycephalum was purified by anion exchange and hydrophobic chromatog

57 The protein was purified by anion- and cation-exchange chromatograph

58 The homohexamers were purified by anion-exchange and gel-filtration chrom

59 Peptides were purified by anion-exchange chromatography and seque

60 plex yields several peptide-DNA adducts that were purified by anion-exchange column chromatography.

61 eal brush border membrane protein (I100) has been purified by anionic glycocholate affinity chromatog

62 Cross-linked peptides were purified by avidin affinity chromatography and char

63 Mutant proteins were purified by avidin affinity chromatography, labeled

64 erial has an altered buoyant density and can be purified by banding in cesium chloride (CsCl) gradien

65 Additionally, the products may be purified by basic extraction or salt formation, avoid

66 to a vegetable field produces a protein that was purified by bioactivity-guided fractionation based o

67 ins interacting with the tr1 promoter region were purified by biotin-labeled DNA affinity chromatogra

68 The complex was purified by both standard biochemical techniques and

69 The glycosylinositol phospholipids were purified by butanol extraction of a Triton X-114-so

70 exchange with (18)F-fluoride, and the tracer was purified by C18 cartridge separation.

71 scherichia coli, and the recombinant protein was purified by CaM affinity chromatography.

72 , which we have named Pseudechetoxin (PsTx), was purified by cation exchange and RP-HPLC and has a mo

73 GUS), and 31 healthy volunteers (normal PCs) were purified by CD138(+) selection.

74 When infected and noninfected cells are purified by cell sorting, the former uniformly expre

75 lator cells, and the CD25(+)-activated cells were purified by cell sorting.

76 ted CD4(+) donor T cells that expressed CD25 were purified by cell sorting.

77 The 90Y-21T-BAD-Lym-1 products were purified by centrifuged molecular sieving column ch

78 lar lysate from this recombinant preparation was purified by cesium chloride density gradient centrif

79 The c subunit was purified by chloroform/methanol extraction and deter

80 Act d 12 and Act d 13 were purified by chromatographic procedures.

81 The heterodimer can be purified by chromatography on nickel-nitriloacetic ac

82 Recombinant E. coli MEP was purified by chromatography on DE-52 and phenyl-Sepha

83 The domains were purified by cloning.

84 f stationary phase, the iodoaziridines could be purified by column chromatography; the use of deactiv

85 Both proteins have been purified by column chromatography to >90% homogenei

86 lyacrylamide gel electrophoresis (SDS-PAGE), was purified by column chromatography and SDS-PAGE and s

87 BChE produced by coexpression with Q(N)PRAD was purified by column chromatography.

88 The byproduct was purified by combined ion exchange and reversed phase

89 A ParE protein derivative, designated ParE', was purified by construction of a fusion protein, GST-Pa

90 TibA was purified by continuous-elution preparative sodium do

91 lectively cross-linked to the functional PLE was purified by conventional chromatography and identifi

92 ance of an ancillary vector in 293 cells and was purified by CsCl banding.

93 Ba1 was purified by CsCl gradient centrifugation and was fou

94 collagenase digestion and size fractionation were purified by CsCl density gradient centrifugation.

95 tite ELP-intein-target protein precursor can be purified by cycles of salt addition, heating and cent

96 The HbRC core was purified by DEAE ion-exchange chromatography and res

97 (Hs antigen) in the parent cell extract that was purified by DEAE Sephacel column chromatography and

98 stance is stable to mild base hydrolysis and was purified by DEAE-cellulose column chromatography.

99 Isolated cells were purified by density gradient.

100 Rat liver mitochondria were purified by differential and Percoll gradient centr

101 Exosomes from conditioned media were purified by differential centrifugation.

102 Islets were purified by discontinuous gradient centrifugation,

103 r from mouse beta TC-3 cell nuclear extracts was purified by DNA affinity chromatography and two-dime

104 The E2 binding protein was purified by DNA affinity chromatography.

105 The murine proteins were purified by DNA affinity isolation from the RAW264.

106 RLIP76 expressed in E. coli was purified by DNP-SG affinity chromatography.

107 Analysis of SCoV particles that were purified by either sucrose gradient equilibrium cen

108 Tia was purified by electroelution of outer membrane protein

109 The secreted proteins were purified by elution from columns of attached antibo

110 B and plasma cells from humans and baboons were purified by FACS sorting and characterized for anti

111 The cytolytic protein was purified by fast protein liquid chromatography (FPLC

112 Oms66 was purified by fast-performance liquid chromatography a

113 Wild-type virions were purified by filtration and exclusion chromatography

114 N'-acyl-N,N-dialkylformamidine intermediates are purified by flash-column chromatography and the puri

115 Rat islets were dispersed and beta-cells were purified by fluorescence-activated cell sorting acc

116 Bone marrow-derived HSCs were purified by fluorescence-activated cell sorting and

117 rom the septal region of late embryonic mice were purified by fluorescence-activated cell sorting bas

118 DCs were purified by fluorescence-activated cell sorting or

119 gammadeltaT cells were purified by fluorescence-activated cell sorting.

120 terygoplichthys disjunctivus viscera trypsin was purified by fractionation with ammonium sulphate, ge

121 ether with tightly bound protein factors can be purified by gel filtration as a functional entity cal

122 This immunoprecipitated fragment was purified by gel electrophoresis and subjected to mic

123 entified keratan sulfate proteoglycan (KSPG) was purified by gel filtration (Superose 6; Amersham Pha

124 The active agent of this venom was purified by gel filtration and reverse-phase high pr

125 Ligand was purified by gel filtration, affinity precipitation o

126 Slow and fast migrating products were purified by gel electrophoresis and imaged by atomi

127 situ, and the telomere restriction fragments were purified by gel filtration chromatography.

128 ked, and the telomeric restriction fragments were purified by gel filtration.

129 PM proteins were purified by gel permeation, anion exchange, and NPA

130 parations of prothymosin alpha, one of which was purified by gentle means (the native protein, and a

131 The extracted polypeptide was purified by glutathione affinity column chromatograp

132 The fusion protein was purified by glutathione affinity, and CLIC-1 was rel

133 The overexpressed proteins were purified by glutathione-Sepharose 4B affinity chrom

134 Total GSTs were purified by GSH-affinity chromatography, and the is

135 00 mg/L of Escherichia coli culture) and can be purified by heat treatment; they can operate up to 75

136 ression levels, His-tagged Aquifex Amt could be purified by heating and nickel chelate affinity chrom

137 y solid-phase method using C18 cartridge and was purified by high performance liquid chromatographic

138 The radiolabeled peptide was purified by high-pressure liquid chromatography and

139 oproteinase Asp-N, and the peptide fragments were purified by high performance liquid chromatography.

140 hese classes with IC(50) values below 600 nm were purified by high pressure liquid chromatography, ch

141 Six peptides were purified by high-performance liquid chromatography

142 rom frozen tissue from two corneas with FSCA were purified by high-pressure liquid chromatography fol

143 t minimize artifacts, individual nucleosides are purified by HPLC and quantified by isotope-dilution,

144 ted with trypsin and the resulting fragments are purified by HPLC.

145 The 185 kDa protein was purified by HPLC, characterized by mass spectrometry

146 alkali and the resulting 7 alpha-[125I]IDHT was purified by HPLC.

147 the photo-dimer-containing oligonucleotides were purified by HPLC (ion exchange and reverse phase) a

148 The cross-linked duplexes were purified by HPLC and characterized by enzymatic dig

149 S12) RNAs with short and long poly(A) tracts were purified by hybrid selection and analyzed by RT-PCR

150 The protease was purified by hydrophobic interaction chromatography o

151 ne retina mixed with radioactive carotenoids were purified by hydrophobic interaction, ion exchange,

152 After expression in E. coli, the scFv was purified by immobilized metal affinity chromatograph

153 The recombinant aspartyl proteinase was purified by immobilized metal affinity chromatograph

154 hydrogenase system of Rhodospirillum rubrum, was purified by immobilized metal affinity chromatograph

155 rypsin, and two ligand-cross-linked peptides were purified by immobilized aluminum affinity chromatog

156 late assay (Alexon, Inc., Sunnyvale, Calif.) was purified by immunoaffinity chromatography from super

157 Recombinant chsp10 was purified by immunoaffinity chromatography with M1.2.

158 The extracts containing the HAAs were purified by immunoaffinity chromatography and analy

159 ss-linking prior to detergent extraction and were purified by immunoaffinity chromatography.

160 Human maltase-glucoamylase was purified by immunoisolation and partially sequenced.

161 Labeled nascent transcripts are purified by immunoprecipitation, and transcript leve

162 Free CrSUMO96 was purified by immunoprecipitation and identified by ma

163 The reactive antigen was purified by immunoprecipitation and microsequenced;

164 . citri adhesion related protein P89 (SARP1) was purified by immunoprecipitation using anti-SARP1 mon

165 minant protein recognized by the immune sera was purified by ion exchange chromatography and reverse

166 ative UDP-l-Ara4N product generated in vitro was purified by ion exchange chromatography, and its str

167 dodecyl beta-D-maltoside, and the RC complex was purified by ion-exchange chromatography.

168 The PigA protein was purified by ion-exchange chromotography.

169 Ncd tail fusion proteins (TrxNT) were purified by ion exchange (S-Sepharose) and/or Talon

170 The aqueous extracts obtained were purified by ion exchange chromatography techniques

171 Subunits were purified by isoelectric focusing from extracts of c

172 Borrelial inner and outer membranes were purified by isopycnic centrifugation, and membrane

173 form of basic fibroblast growth factor (FGF) was purified by its capacity to stimulate proliferation

174 all of the compounds in our druglike library are purified by LC/MS/UV and are then controlled for ide

175 These complexes can be purified by lectin chromatography or by using Ni2(+)-

176 secreted env ectodomain (recombinant gp140) were purified by lentil-lectin chromatography and gel fi

177 olyhedrovirus of Culex nigripalpus (CuniNPV) were purified by Ludox density gradient ultracentrifugat

178 Murine marrow-derived stromal cells (MSCs) were purified by magnetic bead separation of cultured bo

179 ubsets of peripheral blood mononuclear cells were purified by magnetic bead separation, and included

180 th the MBP-SalA and MBP-SyrF fusion proteins were purified by maltose affinity chromatography.

181 gamma-glutamyltransferase [GGT]; EC 2.3.2.2) was purified by means of fast protein liquid chromatogra

182 The protease was purified by means of several chromatography steps.

183 nts of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag.

184 The isotopes were purified by means of cation exchange chromatography

185 tosolic and mitochondrial SODs of C. sapidus were purified by means of ion-exchange, size-exclusion,

186 The resulting Fab/Rev complex was purified by metal ion affinity chromatography and ch

187 polymerase/promoter system, and the protein was purified by metal-chelate affinity chromatography.

188 oximately 48-kDa His-tag derivative proteins were purified by metal affinity chromatography, and thei

189 The labeled proteins are purified by mini-scale affinity chromatography and a

190 e urine samples from the in vivo experiments were purified by mixed-mode cation-exchange solid-phase

191 m inclusion bodies, and the active component was purified by MMP-1 affinity chromatography.

192 cted Sf9 cells, and the recombinant proteins were purified by Mono Q chromatography and studied for e

193 permease containing a biotin acceptor domain was purified by monomeric avidin affinity chromatography

194 isease, retain fitness, and likely would not be purified by mosquito vectors.

195 The Sc2C2@Cs(hept)-C88 was purified by multistage high-performance liquid chrom

196 In this study, a new FMN hydrolase was purified by multistep chromatography after ammonium

197 Rubrerythrin was purified by multistep chromatography under anaerobic

198 ility to hydrolyze N-acetyl-L-methionine and was purified by multistep chromatography.

199 The enzyme was purified by multistep chromatography.

200 The enzyme was purified by multistep column chromatography.

201 Using human umbilical cord, native type XIX was purified by neutral salt extraction and by ion excha

202 o package high-titer viral vectors and could be purified by Ni-nitrilotriacetic acid affinity chromat

203 The recombinant His(6)-tagged TcNr was purified by Ni affinity chromatography.

204 tor with a histidine-tagged carboxy-terminus was purified by Ni-agarose chromatography, and this vari

205 The expressed proteins were purified by Ni-affinity column chromatography to yi

206 The resulting His6-PubC fusion proteins were purified by Ni-NTA affinity and gel filtration chro

207 HA), and the His-tagged recombinant proteins were purified by Ni-NTA chromatography.

208 the expressed peptide from Escherichia coli was purified by Ni2+ affinity chromatography, and rabbit

209 IPP isomerase from Thermus thermophilus HB27 was purified by Ni2+ affinity chromatography.

210 These derivatives were purified by Ni2+ affinity chromatography and examin

211 scherichia coli, and the recombinant protein was purified by nickel affinity chromatography and gel f

212 The IalB fusion protein was purified by nickel affinity chromatography and used

213 -terminal polyhistidine tag, and the protein was purified by nickel affinity chromatography.

214 When histidine-tagged PrgX (His-PrgX) was purified by nickel column chromatography from a stra

215 FqrB was purified by nickel interaction chromatography follow

216 f FliG, His-tagged FliM, FliN, FliH and FliI was purified by nickel-affinity chromatography.

217 scherichia coli, and the recombinant protein was purified by nickel-nitrilotriacetic acid affinity ch

218 Expressed VP5* and truncated VP5* proteins were purified by nickel affinity chromatography and assa

219 The His-tagged proteins were purified by nickel-nitrilo-triacetic acid chromatog

220 in Escherichia coli, and expressed proteins were purified by nickel-nitrilotriacetic acid (Ni-NTA) a

221 The His-tagged proteins were purified by nickel-nitrilotriacetic acid (Ni-NTA) c

222 he second method (ninhydrin/GC/IRMS) the CO2 was purified by on-line GC.

223 el of hybrids, only the final product has to be purified by precipitation or chromatography.

224 on vector, pPROEX-1, and recombinant protein was purified by preparative electrophoresis.

225 These compounds were purified by preparatory high performance liquid chr

226 Amplification System, and the chTNT-3 mutant was purified by protein A affinity and ion-exchange chro

227 LA IgG was purified by protein A chromatography from the plasma

228 Serum immunoglobulin was purified by protein A/G chromatography.

229 IgG was purified by protein-G chromatography, and xenoreacti

230 of low bandgap indacenodithiophene polymers is purified by recycling SEC in order to isolate narrow

231 Crude CDS from bovine lung was purified by reverse-phase high-pressure liquid chrom

232 hemical cleavage, and the resultant peptides were purified by reverse phase high pressure liquid chro

233 he 5-guanidino-4-nitroimidazole modification were purified by reverse-phase and anion-exchange HPLC a

234 The hydroxamates were purified by reverse-phase and size-exclusion chroma

235 Two new theta-defensins, RTD-2 and RTD-3, were purified by reverse-phase high performance liquid c

236 deprotected, modified oligodeoxynucleotides were purified by reverse-phase HPLC and characterized by

237 The eluted peptides were purified by reverse-phase HPLC and tested for their

238 eaction of 3OHKYN and N(alpha)-acetyl lysine was purified by reversed phase high pressure liquid chro

239 ureus V8 protease, and a 3H-labeled fragment was purified by reversed-phase high-performance liquid c

240 The labeled product was purified by reversed-phase high-performance liquid c

241 (18)F-FB-IL2 was purified by reversed-phase high-performance liquid c

242 The new BBN constructs were purified by reversed phase high-performance liquid

243 tive and modified synthetic oligonucleotides were purified by reversed-phase HPLC using volatile ion-

244 The crosslinking activity was purified by RNA affinity chromatography and identifi

245 ntranslated region (3'-UTR) binding proteins were purified by RNA affinity chromatography from cytoso

246 These proteins were purified by RNA affinity column with biotinylated n

247 atic digestion, and peptides containing (3)H were purified by SDS-polyacrylamide gel electrophoresis

248 Most minimotifs have been purified by selection, with a 94% invariance, which

249 protein (OmpH) of Pasteurella multocida X-73 was purified by selective extraction with detergents, fo

250 nd the PLA2 activity in the soluble fraction was purified by Sephacryl S100 and DEAE Sephacel.

251 Pde1 is purified by sequential BioRex-70, PBE118, and MonoS c

252 The interacting protein was purified by sequential DEAE and size exclusion chrom

253 a C-terminally histidine-tagged protein that was purified by sequential metal chelate affinity and ge

254 The smaller PP4 complex was purified by sequential phenyl-Sepharose, Source 15Q,

255 The TAT-Surv proteins were purified by sequential affinity and ion-exchange ch

256 The phosphonoglycans were purified by sequential organic solvent extractions,

257 DEK in supernatants and exosomes was purified by serial centrifugation and immunoprecipit

258 DEK in supernates and exosomes was purified by serial centrifugation and magnetic beads

259 usi spores derived from rhesus macaque feces were purified by serial salt-Percoll-sucrose-iodixanol c

260 Chiral allylboronates could be purified by silica gel chromatography and stored in t

261 th glutathione S-transferase (GST-Ad7PB) and was purified by single-step affinity chromatography.

262 ter solubilization of rPrP in 8 M GdnHC1, it was purified by size exclusion chromatography and revers

263 Bovine hemolysate was purified by size exclusion chromatography, and one h

264 proteinase with a molecular mass of 28.7 kDa was purified by size-exclusion and cation exchange chrom

265 The construct was purified by size-exclusion chromatography and was fo

266 he wild-type (alphaA-wt) and mutant proteins were purified by size exclusion chromatography and chara

267 Urinary LCs were purified by size exclusion chromatography using FPL

268 . coli, and the truncated alphaA-crystallins were purified by size-exclusion chromatography.

269 ated either with VSH-1 capsids or with tails were purified by sodium dodecyl sulfate-polyacrylamide g

270 artridge Extraction (LLCE), the FFAs extract was purified by Solid Phase Extraction (SPE), methylated

271 lite of 8-iso-prostaglandin F(2alpha.) Urine was purified by solid-phase extraction and analyzed by U

272 (99m)Tc was purified by solid-phase extraction or biphasic excha

273 Urine samples were purified by solid-phase extraction columns, reduced

274 enylation of linoleic acid yielded LNO2 that was purified by solvent extraction, silicic acid chromat

275 e marrow hematopoietic stem cells (HSCs) can be purified by sorting Hoechst 33342-extruding side popu

276 idine-tagged actin are coexpressed, they can be purified by standard techniques and then separated us

277 compared to reactions using ligands that had been purified by standard methods.

278 The product was purified by stirring for 5 min with a 20% (w/v) susp

279 ated from the salivary gland of R. prolixus, was purified by strong cation exchange and reverse-phase

280 DESIGN AND ELVs released from adipose tissue were purified by sucrose gradient centrifugation and lab

281 Mutant mouse MDR3 P-glycoproteins were purified by the same procedure after growth of cell

282 The 22:6OOH was obtained from pure 22:6 and was purified by thin-layer and high-performance liquid c

283 an virus 40 transcription elongation complex was purified by this method and the topological linking

284 TGP1 was purified by three-column chromatographies, including

285 Both native and SeMet recombinant proteins were purified by three chromatographic steps, to yield p

286 The complex was purified by two different methods: conventional chro

287 A C-terminal 6xHis-tagged protein was purified by two-column chromatography.

288 sminogen after treatment of intact cells and was purified by two-dimensional gel electrophoresis and

289 ApoA-1 isoforms were purified by two-dimensional gel electrophoresis (2D

290 EVs have traditionally been purified by ultracentrifugation (UC), however UC ha

291 Lp(a) was purified by ultracentrifugation.

292 Nine proteins have been purified by using the system with yields ranging fr

293 FKBP52 was purified by using a prokaryotic expression plasmid c

294 fermentation and cell disruption, BoNT/A(Hc) was purified by using a three-step chromatographic proce

295 The recombinant protein was purified by using amylose-resin affinity and hydroxy

296 The resulting oligosaccharides were purified by using Bio-Gel P4 gel permeation chromat

297 from rabbit hair, and IgE-reactive proteins were purified by using sequential chromatography.

298 lypeptides of molecular masses 25 and 65 kDa were purified by virtue of their ability to specifically

299 otides that contained 16-bp random sequences were purified by vnd/NK-2 affinity column chromatography

300 The fusion protein was purified by zinc-chelate chromatography, and the N-t