ライフサイエンスコーパス: beacon

1 ligonucleotide hybridization (e.g. molecular beacon).

2 tal defects possibly caused by the molecular beacon.

3 be superior to the corresponding Cy3B/DABCYL Beacon.

4 ndmark protein that functions as a molecular beacon.

5 y and dynamic range of a fluorescent aptamer beacon.

6 m the Personal Genome Project in an existing beacon.

7 Relatives can also be identified in the beacon.

8 ce or absence information about alleles in a beacon.

9 ven individual is present in a given genetic beacon.

10 ing a single (universal) strand displacement beacon.

11 nsmitted, in addition to the location of the beacon.

12 son as potential binding pockets and aptamer beacons.

13 s using isotachophoresis (ITP) and molecular beacons.

14 rease the detection sensitivity of molecular beacons.

15 ty ratios reported for traditional molecular beacons.

16 ll four quadrants, they also did not use the beacons.

17 ynthase, suppressor of cytokine signaling 1) beacons.

18 ch probes, MGB-Eclipse probes, and molecular beacons.

19 enosensors exploiting MB-labeled hairpin DNA beacons.

20 that they are important chromatin regulatory beacons.

21 Beacon 10 also shared short sequence segments with other

22 Beacon 10 did not respond strongly to a variety of other

23 orescent double-looped structure, designated Beacon 10.

24 the rational design of cooperative molecular beacons, a widely employed DNA sensor, using a generaliz

25 by changing the sequence of the nucleic acid beacon according to the corresponding aptamer.

26 nt improvement over the equivalent molecular beacon alone.

27 ism of signaling utilized with the unblocked beacons also plays a significant role with the blocked b

28 reatment and associated with a DNA molecular beacon and applied to cancer cell detection by the trans

29 ystem, and this nicks the duplex between the beacon and ligated product while recycling the free liga

30 i PodJ1 protein appears to act as a polarity beacon and localizes to the newer cell pole.

31 s including Taqman, 3'-MGB-Taqman, Molecular Beacon and Scorpion primers.

32 ibrium between stochastically open molecular beacon and target-bound molecular beacon in the detectio

33 spatial cues, rats could select the correct beacon and use that beacon as a guide to the hidden plat

34 Molecular beacons and a 16 nt RNA derived from the Hfq binding sit

35 polymer and various forms of DNA (molecular beacons and its open state; single-stranded DNA and doub

36 so plays a significant role with the blocked beacons and represents a new and effective strategy for

37 h as not allowing anonymous pings of genetic beacons and requiring minimum beacon sizes.

38 The assay consists of six molecular beacons and two oligonucleotide primer pairs, allowing f

39 us and Esophageal Adenocarcinoma Consortium (BEACON), and discovered three loci simultaneously intera

40 Thus, 1 functions as a molecular peptide beacon, and as it is easily taken up by cells, 1 can als

41 onfocal fluorescence spectroscopy, molecular beacons, and a molecular-confinement microfluidic reacto

42 Using a beacon architecture that masks the catalytic template, w

43 or "no." Here, we show that individuals in a beacon are susceptible to re-identification even if the

44 ncrease by 40-200-fold when the nanoparticle beacons are activated by plasmonic coupling, much higher

45 The beacons are based on colloidal gold nanocrystals in two

46 mismatch selectivity, whereas the unblocked beacons are simpler in design.

47 Molecular-beacons are single-stranded hairpin shaped nucleic acid

48 Beacons are web servers that answer allele-presence quer

49 tion systems, including Taqman and Molecular Beacon, are designed for thermal cycling-based DNA ampli

50 could select the correct beacon and use that beacon as a guide to the hidden platform.

51 Using a fluorescent molecular beacon as a reporter, we observed RNA duplex formation w

52 ined from our sequential strand displacement beacon assay are consistent with those obtained from the

53 While the broken beacon assay exhibited a higher limit of detection, it p

54 However, the broken beacon assay provided the significant analytical advanta

55 This new sloppy molecular beacon assay should greatly simplify rifampin resistance

56 We describe a sequential strand displacement beacon assay that is able to quantify specific DNA seque

57 Here, we used a fluorometric RNAP molecular beacon assay to discern partial RNAP-promoter interactio

58 Here, we used an RNAP molecular beacon assay to investigate interdependencies of RNAP in

59 We developed a molecular-beacon assay to study the association between Rv2629 191

60 Using a highly sensitive RNAP beacon assay, we performed quantitative real-time measur

61 w fluorescent assay, which we call a protein beacon assay, will be instrumental in quantitative disse

62 % level that has been reported for molecular beacon assays.

63 adigm that allowed us to distinguish between beacon, associative cue, and place strategies, we invest

64 Therefore, this molecular-beacon based microfluidic system presents a potentially

65 In this context, molecular beacon based sensors have emerged as powerful, no-wash i

66 e context of a possible dissociation between beacon-based and associative-cue-based response learning

67 These results suggest that the molecular beacon-based approach to assess miRNA expression and dis

68 tly blunted nuclease activity in a molecular beacon-based fluorescence assay.

69 This study uses a newly developed molecular-beacon-based helicase assay (MBHA) to investigate how nu

70 Molecular beacon-based helicase assays were used to show that (BIP

71 ing to siRNA sites may not be good sites for beacon-based mRNA detection, and vice versa.

72 The beacon-based PCR assay designed in the present study is

73 -house, rapid, sensitive, specific molecular-beacon-based PCR assay, using primers against pfoB gene

74 A catalytic beacon biosensor is designed by labeling a fluorophore a

75 A new class of molecular beacon biosensors based on the nanostructured aluminum o

76 abel, multicentre, randomised phase 3 study (BEACON; BrEAst Cancer Outcomes with NKTR-102), conducted

77 cluster-DNA conjugate acts like a molecular beacon but is distinguished because the cluster chromoph

78 er analyzed promising variants identified by BEACON by genotyping 10,158 patients with BE and 21,062

79 aptamer variant was then used to construct a beacon by hybridizing fluorophore-labeled aptamer with a

80 es in target-dependent cleavage of molecular beacons by a DNA nicking enzyme, through which one targe

81 We also show that the designed molecular beacon can inhibit the function of miR-430 and cause dev

82 he detection of microRNAs in vivo, molecular beacons can also be employed to inhibit microRNAs in a s

83 Our results show that beacons can disclose membership and implied phenotypic i

84 Here, we demonstrate that molecular beacons can readily distinguish mature- and pre-miRNAs,

85 his method uses a chimeric RNA-DNA molecular beacon (chMB).

86 ecular probe, termed a chameleon NanoCluster Beacon (cNCB), that lights up into different colors upon

87 t signal changes of the target DNA/molecular beacon complex in single droplets.

88 we developed DNA based label-free molecular beacons consisting of two regions: a signal-generating r

89 Furthermore, the targeted nanoparticle-beacon construct, once bound to the target mRNA sequence

90 In a beacon constructed with 65 European individuals from the

91 A molecular beacon containing three PS RNA modifications is designed

92 Over two summers, squirrels captured from beacon-dense and beacon-thin areas were tested in a radi

93 In both years squirrels from beacon-dense populations reached criterion faster than s

94 Since the design of molecular beacons described here is not limited to any specific pr

95 Possible beacon design rules are identified and approaches for en

96 ar beacons (MBs), and identified the optimal beacon design.

97 ingredients are involved: a soluto-inertial "beacon" designed to emit a steady flux of solute over lo

98 l information to inhibit selecting the decoy beacon, despite knowing the platform's actual spatial lo

99 Molecular beacon detection of equilibrium cyclization (MBEC) is a

100 -based amplification and real-time molecular beacon detection of hMPV were performed using a NucliSEN

101 ocal laser scanning microscopy for molecular beacon detection with a robust statistical pipeline for

102 cement for signal amplification in molecular beacon detection.

103 Molecular beacon DNA probes, containing 1-4 pyrene monomers on the

104 r pair attached to the ends of the molecular beacon DNA strand.

105 By using four sloppy molecular beacons, each possessing a different probe sequence and

106 An electrochemically amplified molecular beacon (EAMB) biosensor is constructed using thiolated h

107 otease-activatable smart probes results in a beacon effect that highlights tumors with high TBRs; thi

108 We demonstrate that these delivery beacons effectively bind and compact plasmid (p)DNA into

109 These delivery beacons efficiently deliver pDNA into cultured cells and

110 tive and selective electrochemical molecular beacon (eMB)-based DNA sensor has been developed.

111 sensitive plasmonic DNA biochip in molecular beacon fashion.

112 Using a protein beacon fluorescent method, we identify structural determ

113 nabled by appropriate selection of molecular beacon fluorophores.

114 f the present study was to design an aptamer beacon for fluorescence resonance energy transfer (FRET)

115 conclusion, we designed a FRET-based aptamer beacon for monitoring of an inflammatory cytokine-IFN-ga

116 red the effect of introducing a navigational beacon for rats to use during testing.

117 flammatory signals by serving as a molecular beacon for recruitment of MEKK1, which in turn mediates

118 l-like repressor that can act as a molecular beacon for recruitment of the LSD1-containing CoREST-CtB

119 pport a model in which m(6)A RNA serves as a beacon for the selective, rapid recruitment of Pol kappa

120 This system is engineered into a catalytic beacon for Tl(3+) with a detection limit of 1.5 nM, whic

121 These locations serve as beacons for additional factors, exemplified by liver X r

122 with receptors and targets, mitochondria are beacons for pharmaceutical modification.

123 aptamer-based fluorescence assay ("molecular beacons") for detecting proteins.

124 sequence designed to interact in a molecular beacon format.

125 Quenching efficiency in this "molecular beacon" format is increased to 88%, much closer to the 9

126 oth antigen transport by DCs and the crucial beacon function of CD4(+) cells for autoreactive CD8(+)

127 conveniently large, identifying a subset of "beacon genes" representing the largest changes will iden

128 sed to complete the picture outlined by the "beacon genes." This allow construction of a structured m

129 water maze task using two visually identical beacons, Grin1(DeltaDGCA1) mice were impaired at using s

130 The surface immobilized aptamer beacon had a linear range from 5 to 100 nM and a lower l

131 d displacement of the ligated product by the beacon hairpin is, however, of limited efficiency.

132 ate subunits and the subsequent opening of a beacon hairpin probe by the ligated product.

133 Detection of nucleic acids using molecular beacons has been based on hybridization between target m

134 This class of SERS nanoparticle beacons has novel mechanisms for molecular detection and

135 Furthermore, we found that molecular beacons have the potential to distinguish miRNAs that ha

136 ow (<1 nM) concentrations in vitro, with RNA beacons having the highest detection sensitivity.

137 lecules, using differently labeled Molecular Beacons hybridizing to the same target, reveal a value b

138 Finally, newly designed sloppy molecular beacons identified many different mutations using a smal

139 's and Esophageal Adenocarcinoma Consortium (BEACON) identified risk loci for BE and esophageal adeno

140 molecular beacon and target-bound molecular beacon in the detection of E. coli 16s rRNA targets.

141 ation between target molecules and molecular beacons in a 1:1 stoichiometric ratio.

142 e allele discrimination ability of molecular beacons in a multiplex real-time PCR format.

143 In this work, a "broken beacon" in which the fluor and quencher were located on

144 sis, and assembly of peptide-based molecular beacons into supramolecular protease sensors of either s

145 When compared with morpholino, molecular beacon is 2 orders of magnitude more potent in inhibitin

146 We have shown that if a molecular beacon is linked to a tRNA, it stays in the cytoplasm an

147 oligonucleotide sequences, such as molecular beacons, is that each target strand hybridizes with (and

148 onitored while using only a single molecular beacon-labeled DNA.

149 ular nucleus (IPN) in 3 forms of navigation: beacon, landmark, and path integration.

150 By equipping the hotspot with a molecular beacon-like structure, we combine the plasmonic signal e

151 acon structure, resulting in a LNA molecular beacon (LMB) with robust stability after surface immobil

152 of locked nucleic acid (LNA) based molecular beacons (LNA-MBs) for a variety of applications includin

153 Molecular beacons make attractive probes for imaging mRNA in live

154 e, termed methyladenine-specific NanoCluster Beacon (maNCB), which can detect single m(6)A in DNA tar

155 rants, equidistant from each other (multiple-beacon maze).

156 er in the context of a fluorescent molecular beacon (MB) assay for detection of specific nucleic acid

157 To assess the clinical impact of a molecular beacon (MB) assay that detects multidrug-resistant tuber

158 ncogene as a model DNA molecule, a molecular beacon (MB) containing a 20-base loop, which is compleme

159 A molecular beacon (MB) is comprised of a fluorophore and a quencher

160 use of a novel dimerization-based molecular beacon (MB) probe consisting of two metallo-phthalocyani

161 ghly specific biotinylated DNA/LNA molecular beacon (MB) probe was conjugated with gold nanoparticles

162 s have been used in preparation of molecular beacon (MB) probes labeled with one or two pyrenes/quenc

163 We have combined molecular beacon (MB) probes with barcoded metal nanowires to enab

164 cological target for therapy and a Molecular Beacon (MB) specific for survivin mRNA is available.

165 en OSCS and the adenosine-repeated molecular beacon (MB) stem to coralyne in the presence of Ca(2+) i

166 study presents a thymine (T)-based molecular beacon (MB) used for probing S-adenosylhomocysteine hydr

167 on biosensor based on a label-free molecular beacon (MB) whose DNA hairpin structure terminal has a g

168 fficiency, while a signal reported molecular beacon (MB) with an endonuclease recognition site was de

169 A molecular beacon (MB) with stem-loop (hairpin) DNA structure and w

170 A molecular beacon (MB)-based sensor to offer a decisive answer in c

171 Using molecular beacon (MB)-fluorescent probes to image the viral genomi

172 is and characterization of a novel molecular beacon (MB-FB) which uses the fluorescent bases (FB) 2-a

173 The application of molecular beacons (MB) that bind to precise sequences of mRNA prov

174 mass spectrometry (PCR/ESI-MS) and molecular beacons (MB), for detecting genes conferring resistance/

175 ultiplex real-time PCR assay using molecular beacons (MB-PCR) for rapid and accurate identification o

176 transcripts in living cells using molecular beacons (MBs) - fluorogenic oligonucleotide probes - wit

177 ction of Cu(2+) in solutions using molecular beacons (MBs) and graphene oxide (GO).

178 robes, quantum dot labeled probes, molecular beacons (MBs) and tentacle probes (TPs).

179 The sensing method employs molecular beacons (MBs) and two single-stranded helper DNA probes.

180 olarity-sensitive fluorophores and molecular beacons (MBs) are among the most popular approaches to p

181 Molecular beacons (MBs) are dual-labeled oligonucleotides that flu

182 how that when 2'-O-methyl-modified molecular beacons (MBs) are introduced into NIH/3T3 cells, they el

183 a growing number of studies where molecular beacons (MBs) are used to image RNA expression in living

184 uence, the multiple-pyrene labeled molecular beacons (MBs) assumed a stem-closed conformation resulti

185 lipoplex nanoparticles containing molecular beacons (MBs) can capture cancer cell-derived exosomes o

186 ave a hairpin structure similar to molecular beacons (MBs) for enhanced specificity, but are modified

187 leic acids (LNA) to engineer novel molecular beacons (MBs) for long-term intracellular monitoring.

188 ribe the use of nuclease-resistant molecular beacons (MBs) for the real-time detection of coxsackievi

189 Specifically, molecular beacons (MBs) have been employed for this purpose; howev

190 Molecular beacons (MBs) have recently emerged as efficient probes

191 Molecular beacons (MBs) have shown great potential for the imaging

192 Molecular beacons (MBs) have the potential to provide a powerful t

193 fluorescence signal from 2'-deoxy molecular beacons (MBs) targeting K-ras and GAPDH mRNAs in living

194 de enhanced performance levels for molecular beacons (MBs) that are used for recognizing and reportin

195 Numerous studies have utilized molecular beacons (MBs) to image RNA expression in living cells; h

196 mRNA using 10 different designs of molecular beacons (MBs), and identified the optimal beacon design.

197 on by using two different types of molecular beacons (MBs), MB1 and MB2.

198 labeled, the sequential strand displacement beacon method is able to quantify multiple unlabeled DNA

199 lass of mismatch-tolerant "sloppy" molecular beacons, modified them to provide an extended hybridizat

200 ecause one target molecule converts only one beacon molecule to its fluorescent form.

201 r highlight biosensing applications, aptamer beacon molecules were immobilized inside microfluidic ch

202 , through which one target DNA can open many beacon molecules, giving rise to amplification of fluore

203 target DNA leads to cleavage of hundreds of beacon molecules, increasing detection sensitivity by ne

204 d sequence-based amplification and molecular beacon (NASBA-MB) detection system in multiplex format w

205 landmark version of the water task, but left beacon navigation intact.

206 In beacon navigation, animals reach goals using cues direct

207 NanoCluster Beacons (NCBs) are collections of a small number of Ag a

208 t Pain with Computed Tomography Angiography [BEACON]; NCT01413282).

209 ealed that IFN-gamma binding with an aptamer beacon occurred within 15 min of analyte introduction--s

210 elongates, therefore behaving as a permanent beacon of division sites.

211 l prerequisites of life we call atmospheric "beacons of life" create a unique opportunity to perform

212 ons (CA) and abnormal mitoses are considered beacons of malignancy.

213 ce at the edges of the disturbance produced "beacons" of infrared emission and longitudinal temperatu

214 The blocked beacons offer better mismatch selectivity, whereas the u

215 Both the blocked and unblocked beacons offer nanomolar (femtomole) detection limits, re

216 e structure-switching biosensor, a molecular beacon, over 4 orders of magnitude.

217 ls (Urocitellus beldingi) from areas rich in beacons perform differently in a task of spatial memory

218 Because of their versatility, these delivery beacons posses remarkable potential for tracking and und

219 Sloppy molecular beacons possess relatively long probe sequences, enablin

220 The sensor consists of a molecular beacon probe as a signal reporter and two DNA adaptor st

221 lopment of a fluorescence turn-on "molecular beacon" probe for the detection of glutathione (GSH) and

222 Molecular beacon probes (MBs) were used to demonstrate these point

223 ctures analogous to solution-phase molecular beacon probes are relatively insensitive to hybridizatio

224 24 bases and 5 base pair stem regions in the beacon probes gave optimal performance.

225 ort here the use of novel "sloppy" molecular beacon probes in homogeneous PCR screening assays in whi

226 veloped a platform using multiplex molecular beacon probes with real-time PCR for the rapid detection

227 imaging probe called ratiometric bimolecular beacon (RBMB), which combines functional elements of bot

228 leotide-based probe, ratiometric bimolecular beacon (RBMB), which generates a detectable fluorescent

229 We developed a rapid molecular beacon real-time PCR (MB-PCR) assay for staphylococcal c

230 A species-specific molecular beacon real-time PCR assay was developed for rapid diagn

231 ng l-DNA into the stem region of a molecular beacon reduces intra- and intermolecular stem invasions,

232 Molecular beacons represent a new family of fluorescent probes for

233 Conventional molecular beacons require double-labeled DNA sequences, which are

234 target sequence to form a reverse molecular beacon (rMB), thus bringing Cy5 (donor) and Cy5.5 (accep

235 cy of LMBs against that of regular molecular beacons (RMBs), the properties of selectivity, sensitivi

236 ported in BE cases 3 risk SNPs identified by BEACON (rs2687201, rs11789015, and rs10423674).

237 or of ideal behavior, greatly steepening the beacon's binding curve relative to that of the parent re

238 This is mainly a consequence of the beacon's poor stability because of the hairpin structure

239 Here, we report a catalytic beacon sensor for uranyl (UO2(2+)) based on an in vitro-

240 This is a new DNAzyme, and a catalytic beacon sensor is designed by attaching a fluorophore/que

241 A catalytic beacon sensor was designed with a detection limit of 1.6

242 w class of surface-enhanced Raman scattering beacons (SERS beacons) that can be turned on and off by

243 A primer-molecular beacon set targeting 28S rRNA was designed to detect Asp

244 4 mRNA using siRNA significantly reduced the beacon signals, demonstrating detection specificity.

245 ngs of genetic beacons and requiring minimum beacon sizes.

246 eloped a rapid, multiplexed sloppy molecular beacon (SMB) assay to identify these mutations and then

247 ollowed by probing with two sloppy molecular beacons (SMBs) spanning the entire QRDR.

248 cement of an oligonucleotide-bound molecular beacon so that rates of helicase translocation can be di

249 The beacon specificity was further confirmed using blocking

250 the dehybridization and dissociation of the beacon stem T-Hg(2+)-T due to the extraction of Hg(2+) i

251 ches in a DNA strand of the self-hybridizing beacon strand.

252 Instead, they continuously used a beacon strategy and showed no evidence of changing their

253 The specific preference for a beacon strategy is discussed in the context of a possibl

254 In this work, we utilized a molecular beacon strategy to examine the stability of the (CAG)(10

255 adds locked nucleic acid bases (LNAs) to the beacon structure, resulting in a LNA molecular beacon (L

256 In the presence of target, the beacons switched to a stem-open conformation, which sepa

257 A comparison with aptamer beacon systems indicated that nanoparticles may play an

258 R assays with as few as six sloppy molecular beacons targeting bacterial 16S rRNA gene segments could

259 on (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficienc

260 lation-sensitive endonucleases and molecular beacon technology.

261 s 1000-fold less accessible to the molecular beacon than an unstructured oligonucleotide, and Hfq acc

262 probes, we have created a modified molecular beacon that incorporates unnatural enantiomeric l-DNA in

263 Here, we have developed polymer beacons that allow the delivery of nucleic acids to be v

264 leosome asymmetries might serve as molecular beacons that guide transcription.

265 igns for light-up nucleic acid hybridization beacons that utilize time-resolved Forster resonance ene

266 face-enhanced Raman scattering beacons (SERS beacons) that can be turned on and off by long-range pla

267 rs, squirrels captured from beacon-dense and beacon-thin areas were tested in a radial maze intersper

268 spatial memory compared with squirrels from beacon-thin areas.

269 reached criterion faster than squirrels from beacon-thin populations, and a weak rearing effect was p

270 nealing of a fluorescently labeled molecular beacon to a 16-nt RNA.

271 This study is aimed at using a molecular beacon to detect miR-430 in developing zebrafish embryos

272 Pioneering a novel RNA beacon to illuminate RNA trafficking in neurons, Alami e

273 EC, we propose that DHMA acts as a molecular beacon to target pathogens to their preferred sites of i

274 s process results in switching the molecular beacon to the "on" state.

275 tly from bacterial lysate and used molecular beacons to achieve detection of bacteria specific sequen

276 of a real-time PCR assay utilizing molecular beacons to assess triazole resistance markers in A. fumi

277 d the incorporation of hairpin DNA molecular beacons to confer high specificity and sensitivity to th

278 rotein, it will be possible to develop these beacons to detect a variety of target proteins of biomed

279 rs (MMCs) were functionalized with molecular beacons to enable miRNAs recognition and magnetic separa

280 Probe tests indicated that rats did use the beacons to guide performance throughout training.

281 The assay uses sloppy molecular beacons to probe an asymmetric PCR of the M. tuberculosi

282 we describe two methods of linking molecular beacons to tRNA and show how the joint molecules can be

283 re tested in a radial maze interspersed with beacons, using number of trials to criterion as a measur

284 ty DNA in ice derived from Mullins and upper Beacon Valleys.

285 lization of the amplified product, molecular-beacon was designed and amplicons were visualized direct

286 A DNAzyme beacon was engineered detecting down to 1.7 nM Ce(3+) (2

287 utation detection, and a dual Cy3B Molecular Beacon was synthesized and found to be superior to the c

288 One of the beacons was always directly over the hidden platform.

289 Molecular beacons were designed to hybridize with either type of m

290 pturing probes and fluorescent DNA molecular beacons were developed to rapidly detect NNV and iridovi

291 Sloppy molecular beacons were exceptionally versatile, and they were able

292 Four identical beacons were hung directly over each of the water maze q

293 These candidate beacons were synthesized with 5'-TYE 665 and 3'-Iowa Bla

294 The new beacons were tested with MDA-MB-231 cancer cells and use

295 duced distal allosteric sites into molecular beacons, which are optical sensors for the detection of

296 Through simulations, we showed that in a beacon with 1,000 individuals, re-identification is poss

297 t it is possible to detect membership in the beacon with just 250 SNPs.

298 We found that molecular beacons with DNA, RNA and combined locked nucleic acid (

299 ative to, for example, traditional molecular beacons without any significant restriction in the choic

300 This beacon yielded a lower limit of detection of 1 ng/mL in