ライフサイエンスコーパス: beta-1,3-glucan

1 aniline blue, which is specific for callose (beta-1,3-glucan).

2 protein Bgs4 synthesizes the main cell wall beta(1,3)glucan.

3 eptum was free, with the remainder linked to beta(1-3)glucan.

4 ttached to beta(1-6)glucan and the latter to beta(1-3)glucan.

5 nts being alpha-mannan, beta-1,6 glucan, and beta-1,3 glucan.

6 , has a defective cell wall due to decreased beta-1,3-glucan.

7 nd beta-glucosidase to release the remaining beta-1,3-glucan.

8 increases nearly 100-fold in the presence of beta-1,3-glucan.

9 -betaGRP2) with laminarin, a soluble form of beta-1,3-glucan.

10 mocyte suspensions in the presence of LPS or beta-1,3-glucan.

11 s in the phloem in plants contain callose, a beta-1,3-glucan.

12 factors into recognition and degradation of beta-1,3-glucans.

13 establish cross-links between beta-1,6- and beta-1,3-glucans.

14 kely the triple helix adopted by polymerized beta-1,3-glucans.

15 attenuates the axon-regenerative effects of beta(1, 3)-glucan.

16 a-(1,3)-oligomers from dimer up to insoluble beta-(1,3)-glucan.

17 Da consistent with the repeating unit of the beta-(1-->3)-glucan.

18 o a two-chain active form in the presence of beta-1,3-glucan (a fungal cell wall component) and beta-

19 To target beta-(1,3)-glucan, a structural component of the Pneumoc

20 With both oral beta-1,3-glucans, a requirement for iC3b on tumors and C

21 tivation is required for TLR9 trafficking to beta-1,3 glucan-, A. fumigatus-, and C. albicans-contain

22 Because beta-1,3 glucans activate host antifungal pathways via t

23 ences in surface-accessible MAMPs, including beta-(1,3)-glucan, alpha-mannose, chitin, and other carb

24 The unmasking of beta(1-3)-glucan also results in increased elicitation o

25 -like receptor has shown to recognize fungal beta (1,3)-glucans and induce innate immune responses.

26 etermine whether biofilm cells secreted more beta -1,3 glucan and whether these differences could be

27 minations of cell wall-synthesizing enzymes (beta(1 --> 3)glucan and chitin synthases) and cytosolic

28 polysaccharides in their cell walls of which beta(1,3)-glucan and chitin are of principle importance.

29 ll wall, beta(1-->6)glucan is linked to both beta(1-->3)glucan and mannoprotein, as well as occasiona

30 eins (mannan) and an inner layer enriched in beta-(1,3)-glucan and chitin.

31 macrophages via interactions between fungal beta-(1,3)-glucan and the host receptors Dectin-1 and CD

32 , but was inhibited by high molecular weight beta-(1-3)-glucans and by a monoclonal antibody to lacto

33 beta-1,3-Glucan and chitin are the most prominent polysa

34 caspofungin inhibits synthesis of cell wall beta-1,3-glucan and is used for prophylactic therapy in

35 ndicated that recombinant (r)PmLGBP binds to beta-1,3-glucan and LPS with a dissociation constant of

36 t is suggested that the Phr proteins process beta-1,3-glucans and make available acceptor sites for t

37 at the mother-bud neck, partially linked to beta(1-3)glucan, and in the lateral wall, attached in pa

38 ed an average of 5% chitin, 20% chitosan, 5% beta-(1,3)-glucan, and 70% beta-(1,6)-glucan.

39 beta-(1 --> 3)-Glucans are natural polysaccharides well-

40 is In the current study, we examined whether beta-1,3-glucans are masked by surface proteins in Pneum

41 C. glabrata has higher surface levels of beta-1,3-glucans as compared with C. albicans; however t

42 d cell wall changes (specifically, increased beta -1,3 glucan) associated with biofilm, compared with

43 followed shortly thereafter by a decline in beta-1,3-glucan-associated beta-1, 6-glucans and, within

44 The incorporation of [(14)C]-glucose into beta(1-->3)glucan at 37 degrees C was decreased or aboli

45 ieved by a preferential binding of chitin to beta(1-3)glucan at that site.

46 cked by pathogens rapidly deposit callose, a beta-1,3-glucan, at wound sites.

47 In this study, we demonstrate that beta-1,3 glucan beads are sufficient to induce dynamic r

48 s TLR9 accumulation on phagosomes containing beta-1,3 glucan beads.

49 n available biomass substrate, in this case, beta-1-3 glucan, because both CelC and LicA are active o

50 four structural components of the cell wall, beta(1-->3)-glucan, beta(1-->6)-glucan, chitin, and mann

51 nclude that the order of addition in vivo is beta(1-->3)glucan, beta(1-->6)glucan, mannoprotein.

52 ut does not bind chitosan, cellulose, xylan, beta-1, 3-glucan, beta-1,3-1,4-glucan, or mannan.

53 tion proteins such as lipopolysaccharide and beta-1,3-glucan binding protein (LGBP) play an important

54 (PRPs), including a lipopolysaccharide- and beta-1,3-glucan-binding protein (LGBP).

55 th the face of one beta-sheet possessing the beta-1,3-glucan-binding surface.

56 In this study, laminarin (beta-1,3-glucan) but not sialic acid, mannan or pustulan

57 stulan, laminarin, or a low molecular weight beta-(1-3)-glucan, but was inhibited by high molecular w

58 , our study demonstrates that recognition of beta-1,3 glucan by Dectin-1 triggers TLR9 trafficking to

59 olymph of Manduca sexta, upon the binding of beta-1,3-glucan by its recognition protein, betaGRP2.

60 As beta(1-->3)glucan can be synthesized in vitro or in vivo

61 sglycosylation and driving the elongation of beta(1-3) glucan chains in the yeast cell wall.

62 ansglycosylase activity, producing elongated beta(1-3) glucan chains.

63 dule (CBM) that binds the nonreducing end of beta-1,3-glucan chains, and an uncharacterized C-termina

64 report here on the synthesis of small oligo-beta-(1 --> 3)-glucans characterized by thioglycosidic l

65 ata contained significantly higher levels of beta-1,3-glucans compared with C. albicans, but it did n

66 gand-induced self-association of the betaGRP-beta-1,3-glucan complex may form a platform on a microbi

67 )/beta(1-->4) mixed-linkage glucan (MLG) and beta(1-->3) glucan components of lignocellulose represen

68 s in Pneumocystis carinii have characterized beta-1,3 glucan components of the organism.

69 in and chitosan are relatively abundant, and beta-(1,3)-glucans constitute a minor cell wall componen

70 ectin-1 not only controls internalization of beta-1,3-glucan containing cargo and triggers proinflamm

71 can by Dectin-1 triggers TLR9 trafficking to beta-1,3 glucan-containing phagosomes, which may be crit

72 Massive beta-1,3-glucan contents were detected in cell walls of

73 Unexpectedly, GLS1 expression and beta-1,3-glucan contents were drastically reduced during

74 t and was reproduced by stimulation with the beta(1,3) glucan curdlan, indicating that dectin-1, rath

75 ased activity of the enzyme on the insoluble beta-1,3-glucan curdlan but not on soluble laminarin; ad

76 beta(1-3)-glucan deposition was increased in lrg1Delta s

77 mune cells and bone marrow-derived cells for beta(1, 3)-glucan-elicited optic nerve regeneration.

78 the mechanisms these enzymes employ to drive beta(1-3) glucan elongation.

79 In later studies, beta(1-->3)glucans, extracellular polysaccharides, and m

80 These granulocytes with CR3-bound beta-1,3-glucan-fluorescein were shown to kill iC3b-opso

81 large beta-1,3-glucans into smaller soluble beta-1,3-glucan fragments that were taken up by the CR3

82 However, the concentrations of beta -1,3 glucan from the biofilm conditions were 4-10-f

83 n layer of the wall masks the inner layer of beta(1-3)-glucan from exposure and detection by innate i

84 PS results in decreased masking of cell wall beta(1-3)-glucan from the immune system.

85 vities of these enzymes in the hydrolysis of beta-1,3 glucans from fungal cell walls.

86 and trimeric hydroxylamine-based mimetics of beta-(1-->3)-glucans have been accessed by an asymmetric

87 CR3 is a known receptor for beta-1,3-glucan; however, blocking CR3 had significant e

88 A limulus lysate assay was used to quantify beta -1,3 glucan in supernatants from planktonic or biof

89 -copy LRG1 suppressed the mislocalization of beta(1-3) glucan in fus2Delta strains.

90 addition, it is known to produce paramylon (beta-1,3-glucan in a crystalline form) as reserve polysa

91 as a pattern recognition protein for LPS and beta-1,3-glucan in the shrimp proPO activating system.

92 fied cellulose synthase preparations yielded beta-1,3-glucan in vitro, leading to the interpretation

93 Synthesis of callose (beta-1,3-glucan) in plants has been a topic of much deba

94 We show that C. albicans and beta-1,3-glucan induce priming of human primary mononucl

95 e marrow, the macrophages degraded the large beta-1,3-glucans into smaller soluble beta-1,3-glucan fr

96 Moreover, Bgs4-derived beta(1,3)glucan is essential for secondary septum format

97 We show that Bgs4-derived beta(1,3)glucan is required for correct and stable actom

98 erefore, our results show that extracellular beta(1,3)glucan is required for cytokinesis to connect t

99 The mode of action of SUN proteins on beta-(1,3)-glucan is unique, new, and original.

100 beta-1,3-glucan is a major cell wall component of Pneumo

101 Because synthesis of beta-1,3-glucan is absent in mammalian cells, inhibition

102 nt infection is well understood, the role of beta-1,3-glucan is unknown.

103 Callose (beta-1,3-glucan) is produced at different locations in r

104 in-1, a C-type signaling lectin specific for beta-(1,3)-glucan, is important for the innate immune sy

105 greater degree of polymerization required in beta-(1-->3)-glucans, is discussed in terms of the incre

106 this latter CBM, BhCBM56, bound the soluble beta-1,3-glucan laminarin with a dissociation constant (

107 Particulate beta(1, 3)-glucan leads to increased Erk1/2 MAP-kinase s

108 r C. glabrata cell surface or biofilm matrix beta-1,3-glucan levels affected Hst 5 toxicity; rather t

109 meric and the Ag recognition site identifies beta-1,3 glucan linkages specifically and with high affi

110 e first 181 amino-terminal residues bound to beta-1,3-glucan, lipopolysaccharide, and lipoteichoic ac

111 Consequently, beta(1,3)glucan loss generated ring sliding, oblique pos

112 results suggest the secreted polysaccharide beta -1,3 glucan may serve as a useful tool for the diag

113 tutive expression of GLS1 led to exposure of beta-1,3-glucan on biotrophic hyphae, massive induction

114 eceptor for innate immune responses, detects beta-1,3-glucan on fungal surfaces via its N-terminal ca

115 chitin is free and the remainder attached to beta(1-3)glucan or beta(1-6)glucan.

116 beta-1,3-glucan phagosomes expressing a signaling incomp

117 Laminarin, a beta-1,3-glucan, presented two classes of binding sites

118 ctin-1 ligand curdlan [a particulate form of beta(1, 3)-glucan] promotes optic nerve regeneration com

119 This priming requires the beta-1,3-glucan receptor dectin-1 and the noncanonical R

120 The role of Dectin-1, a beta-1,3-glucan receptor, critical for fungal recognitio

121 response to invading microorganisms, insect beta-1,3-glucan recognition protein (betaGRP), a soluble

122 functional properties of two domains from a beta-1,3-glucan recognition protein present in the hemol

123 ults indicate that the two domains of Plodia beta-1,3-glucan recognition protein, separated by a puta

124 ,3-glucan (a fungal cell wall component) and beta-1,3-glucan recognition protein-2.

125 Following beta-1,3-glucan recognition, GFP-Dectin-1 undergoes tyro

126 Beta (1,3)-glucans represent 40% of the cell wall of the

127 soluble immunomodulator, beta-(1,6)-branched beta-(1,3)-glucan (soluble beta-glucan), on toxin-stimul

128 le GluB is most active against the insoluble beta-1,3 glucan substrate zymosan A.

129 vity of recombinant proteins against various beta-1,3 glucan substrates indicates that GluA and GluC

130 G protein, Rho1, is required for activity of beta (1-->3)glucan synthase, the enzyme that catalyzes t

131 the GTP-binding protein Rho1 is required for beta(1-->3)glucan synthase activity, for activation of p

132 The in vitro activity of beta(1-->3)glucan synthase in rho1 (E45I), although dimi

133 C (Pkc1p) and for activity and regulation of beta(1-->3)glucan synthase.

134 the GTPase activity of Rho1p, a regulator of beta(1-3)-glucan synthase in vitro.

135 which harbor an S645Y mutation in the CaFks1 beta-1,3 glucan synthase drug target, suggesting potenti

136 erol biosynthesis, exhibits synergy with the beta-1,3 glucan synthase inhibitor caspofungin or the ca

137 iptional activation of FKS2, which encodes a beta-1,3 glucan synthase.

138 e homology with the well-characterized yeast beta-1,3-glucan synthase and transgenic plant cells over

139 We functionally characterized the beta-1,3-glucan synthase gene GLS1 of the maize (Zea may

140 after mice were treated with caspofungin, a beta-1,3-glucan synthase inhibitor that is known to redu

141 a chitin synthase inhibitor), caspofungin (a beta-1,3-glucan synthase inhibitor), or FK506 (a calcine

142 nd FKS2 genes, which encode a subunit of the beta-1,3-glucan synthase, the target of echinocandins.

143 iated with the cell wall biosynthesis enzyme beta-1,3-glucan synthase.

144 persensitive to caspofungin, an inhibitor of beta-1,3-glucan synthase.

145 or about cooperation between the alpha- and beta(1-3)glucan synthases Ags1 and Bgs for cell wall and

146 Acylated cyclic peptide inhibitors of beta(1,3)glucan synthesis with origins as fungal metabol

147 appeared to be specifically defective in the beta(1-->3) glucan synthesis function of Rho1p.

148 use of a temperature-sensitive mutation and beta(1-->3)glucan synthesis abolished by an echinocandin

149 lipopeptide molecules that are inhibitors of beta-(1,3)-glucan synthesis, an action that damages fung

150 Echinocandins target fungal beta-1,3 glucan synthesis and are used clinically to tre

151 e involved in cell wall biogenesis, restores beta-1,3-glucan synthesis and suppresses pgs1Delta tempe

152 pressoria of RNAi strains, downregulation of beta-1,3-glucan synthesis increased cell wall elasticity

153 Thus, while beta-1,3-glucan synthesis is required for cell wall rigi

154 Pneumocandin antagonists of beta-1,3-glucan synthesis rapidly suppress infection in

155 ent fungal cell wall synthesis by inhibiting beta-1,3-glucan synthesis, a significant glucose-consumi

156 treatment with caspofungin, an inhibitor of beta-1,3-glucan synthesis, for 21 days decreased express

157 nd responses to echinocandins, which inhibit beta-1,3-glucan synthesis.

158 rinii contains a unique catalytic subunit of beta-1,3-glucan synthetase utilized in cyst wall formati

159 g, the P. carinii Gsc-1 catalytic subunit of beta-1,3-glucan synthetase was cloned and characterized.

160 homology to phylogenetically related fungal beta-1,3-glucan synthetases, encoding a predicted 214-kD

161 for the major fungal cell wall carbohydrate beta-1,3 glucan that induces inflammatory cytokines and

162 Callose, a beta-1,3-glucan that is widespread in plants, is synthes

163 ith beta-1,6 branches, but not to curdlan, a beta-1,3-glucan that lacks branching.

164 ected to the nonreducing terminal glucose of beta(1-->3)-glucan through a linkage that remains to be

165 Increased exposure of beta-(1,3)-glucan to the immune system occurs when the m

166 d that the binding of lipopolysaccharide and beta-1,3-glucan to LGBP activates the prophenoloxidase (

167 MLG and sophorose utilization, and supports beta(1-->3) glucan utilization, while Bgl3B underpins ce

168 Bgl3C drives beta(1-->3) glucan utilization.

169 beta -1,3 glucan was detected from both in vitro and in

170 study, the linkage region between chitin and beta(1-->3)-glucan was solubilized and isolated in the f

171 Uptake into macrophages stimulated with beta-1,3-glucan was blocked 50% by PS liposomes and 40%

172 Barley and yeast beta-1,3-glucan were labeled with fluorescein to track t

173 eumocystis carinii, and Pneumocystis murina, beta-1,3-glucans were masked in most organisms, as demon

174 Orally administered beta-1,3-glucans were taken up by macrophages that trans

175 lta mutant exhibits increases in exposure of beta(1-3)-glucan, which leads to greater binding by Dect

176 C. albicans results in decreased masking of beta(1-3)-glucan, which may contribute to our understand

177 wall of pgs1Delta contained markedly reduced beta-1,3-glucan, which was restored in the suppressor.

178 GluA and GluC are most active against linear beta-1,3 glucans, while GluB is most active against the

179 rboxyl-terminal domain bound to laminarin, a beta-1,3-glucan with beta-1,6 branches, but not to curdl

180 and displayed higher affinity for insoluble beta-1,3-glucans with Kd values of approximately 2-10 mu