ライフサイエンスコーパス: beta-mercaptoethanol

1 The enzymatic activity was increased by beta-mercaptoethanol.

2 cles was denatured and reduced with urea and beta-mercaptoethanol.

3 rom the radical scavengers ascorbic acid and beta-mercaptoethanol.

4 biotinylation occurred after treatment with beta-mercaptoethanol.

5 ucted 18:1-NO2 detected by exchange to added beta-mercaptoethanol.

6 the protein was denatured in the presence of beta-mercaptoethanol.

7 zed online with o-phthaldialdehyde (OPA) and beta-mercaptoethanol.

8 ng in air, but not in the presence of 100 mM beta-mercaptoethanol.

9 stable in the presence of heating, SDS, and beta-mercaptoethanol.

10 ester bond by removal with dithiothreitol or beta-mercaptoethanol.

11 r was highly stable and resistant to SDS and beta-mercaptoethanol.

12 were also inhibited by DMSO and reversed by beta-mercaptoethanol.

13 atured by boiling in the presence of SDS and beta-mercaptoethanol.

14 g 6 m guanidine HCl, 8 m urea, 2% SDS, or 5% beta-mercaptoethanol.

15 tigen was denatured by heat or reduced using beta-mercaptoethanol.

16 agents such as 5 mM dithiothreitol or 10 mM beta-mercaptoethanol.

17 and occurred in the presence of an excess of beta-mercaptoethanol.

18 the mercurial inhibition was reversible with beta-mercaptoethanol.

19 onated by DEAE chromatography in 6 M urea/4% beta-mercaptoethanol.

20 essential amino acids, fetal calf serum, and beta-mercaptoethanol.

21 Trapping of 1 by O2 or beta-mercaptoethanol (1 M) does not compete with strand

22 man erythrocytes included: pronase, trypsin, beta-mercaptoethanol (2-ME), and heating to 90 degrees .

23 turation by sodium dodecyl sulfate (SDS) and beta-mercaptoethanol (2ME), suggesting the recognition o

24 Treatment of DENV-2 with beta-mercaptoethanol abolished binding of Fab 1A5, indic

25 p1 can be released, trapped, and detected as beta-mercaptoethanol adducts by mass spectrometry.

26 eosin-5-maleic acid, and the L-cysteine and beta-mercaptoethanol adducts of EM in aqueous and hydrop

27 curate mass measurement was used to identify beta-mercaptoethanol adducts of sulforaphane that had be

28 reatment of alpha1PI with the reducing agent beta-mercaptoethanol also inhibited binding of trypsin t

29 the thiol-reducing agents dithiothreitol and beta-mercaptoethanol also inhibited high affinity [3H]ry

30 derivatized on-line with o-phthaldialdehyde/beta-mercaptoethanol and automatically transferred to a

31 than 100-fold higher than that observed for beta-mercaptoethanol and cysteine, suggesting that thior

32 derivatized on-line with o-phthaldialdehyde/beta-mercaptoethanol and detected by LIF using the 354 n

33 se signature-events were abolished by 100 mM beta-mercaptoethanol and did not occur in a cysteineless

34 Data analysis shows that beta-mercaptoethanol and ethanol both interact or bind p

35 ended in 2% sodium ascorbate containing 10mM beta-mercaptoethanol and heated to release the folates.

36 treatment with 2% sodium dodecyl sulfate-1% beta-mercaptoethanol and heating to 100 degrees C for 5

37 with derivatization by o-phthalaldehyde and beta-mercaptoethanol and optically gated capillary elect

38 ysteine, homocysteine, cysteinylglycine, and beta-mercaptoethanol) and human serum albumin.

39 eotides, a reducing agent (dithiothreitol or beta-mercaptoethanol), and Mg2+ and was resistant to inh

40 ed by thiol reagents, including glutathione, beta-mercaptoethanol, and dithiothreitol.

41 mined the solvent effects of a co-substrate, beta-mercaptoethanol, and of a model nonsubstrate, ethan

42 d with proteinase K, boiled in detergent and beta-mercaptoethanol, and subjected to sucrose density g

43 reated with three different reductants (DTT, beta-mercaptoethanol, and TCEP).

44 ative thiols methyl-3-mercaptopropionate and beta-mercaptoethanol are shown to add exclusively and qu

45 Dioxygen and beta-mercaptoethanol are unable to compete with loss of

46 n of amino acid derivatives, generated using beta-mercaptoethanol as a nucleophile, was characterized

47 sulfhydryl compounds such as l-cysteine and beta-mercaptoethanol as cosubstrates.

48 Urea, ethanol, and beta mercaptoethanol (beta-ME) were used to evaluate the

49 three reducing agents-dithiothreitol (DTT), beta-mercaptoethanol (beta-MCE), and tris(2-carboxyethyl

50 These mutants produce a beta-mercaptoethanol (beta-ME) adduct of the 2'-deoxyuri

51 peptide linkage by 1,4-dithiothreitol (DTT), beta-mercaptoethanol (beta-ME) or cysteine at low temper

52 Transient treatment with the reductant beta-mercaptoethanol (beta-ME) was able to partially res

53 ur different photostabilizing agents, namely beta-mercaptoethanol (beta-ME), Trolox (TX), n-propyl ga

54 tivity can be restored by the treatment with beta-mercaptoethanol (beta-ME).

55 The reducing agent beta-mercaptoethanol (betaME) reversibly decreased the a

56 ed blue-fluorescent state by incubation with beta-mercaptoethanol (betaME).

57 ic circular dichroism spectra of the H93G Mb beta-mercaptoethanol (BME) thiolate adduct reveal a high

58 e-based kinetic studies of the reaction with beta-mercaptoethanol (BME) yielded the second-order rate

59 In the presence of 30 mM beta-mercaptoethanol (BME), the k(cat) increased to 176

60 Our results show that ethanol and beta-mercaptoethanol both alter the equilibrium distribu

61 d to be co-localized on immunoblots of yeast beta-mercaptoethanol cell wall extracts and cytosolic fr

62 7,8-Dihydrobiopterin and other thiols (E.G., beta-mercaptoethanol, cysteine, and glutathione, with le

63 Higher concentrations of beta-mercaptoethanol decrease the concentration of the q

64 We encountered beta-mercaptoethanol-dependent artifact signals in weste

65 Treatment with beta-mercaptoethanol did not impact the apparent molecul

66 Three types of beta-mercaptoethanol-dissociable complexes were visualiz

67 g SDS, urea, glycerol, ammonium sulfate, and beta-mercaptoethanol, do not interfere with this method.

68 e protects the enzyme from inactivation, but beta-mercaptoethanol does not, suggesting that the 6 bet

69 e protects the enzyme from inactivation, but beta-mercaptoethanol does not, suggesting that these com

70 he concentration of either dithiothreitol or beta-mercaptoethanol eliminated the background problems

71 identified a new spore protein, ExsM, from a beta-mercaptoethanol extract of B. cereus ATCC 4342 spor

72 The moiety was present in the beta-mercaptoethanol extracts of cell walls from both bl

73 n the other hand, neither dithiothreitol nor beta-mercaptoethanol had any effect on the N-SMase, sugg

74 side group, an external thiolate provided by beta-mercaptoethanol has likely been recruited to supply

75 anate from trapping of the intermediate with beta-mercaptoethanol in competition with hydride transfe

76 and thiol reducing agents dithiothreitol and beta-mercaptoethanol inactivated the enzyme.

77 Ag(2+) and beta-mercaptoethanol increased while SDS and EDTA inhibi

78 eluted from a phenylarsine oxide matrix with beta-mercaptoethanol indicating that they contain vicina

79 The radical abstracts hydrogen atoms from beta-mercaptoethanol (k = 8.8 +/- 0.5 x 10(6) M(-)(1) s(

80 Low concentrations of the co-substrate beta-mercaptoethanol (Kd = approximately 13 mM) decrease

81 d be rescued from l-Hcy-induced apoptosis by beta-mercaptoethanol medium supplementation that increas

82 A structure with the loop occupied with beta-mercaptoethanol mimics binding of MAs(III).

83 Hydrogen atom transfer to 1 from beta-mercaptoethanol occurs exclusively from the alpha-f

84 > Mn2+ > Mg2+; no inhibition was found with beta-mercaptoethanol or dithiothreitol.

85 addition of metal complexing agents such as beta-mercaptoethanol or imidazole to the reaction buffer

86 chains were mixed with 300 x molar excess of beta-mercaptoethanol over the p-hydroxymercuribenzoate g

87 th 10 mM phosphate buffer-1 mM Na2EDTA-47 mM beta-mercaptoethanol, pH 5.85 and then with 10 mM phosph

88 Treatment of the labeled samples with beta-mercaptoethanol prior to SDS-PAGE removed the disul

89 yme preparations, such as dithiothreitol and beta-mercaptoethanol, probably preserve enzyme activity

90 Treatment with beta-mercaptoethanol removed all radiolabel.

91 Supplementation of culture media with beta-mercaptoethanol rescues CD98hc-deficient cell survi

92 High molecular mass (>200 kDa), SDS- and beta-mercaptoethanol-resistant Fas aggregates were forme

93 Reduction of this disulfide bond by beta-mercaptoethanol restores activity, indicating that

94 ion of a differentiation protocol containing beta-mercaptoethanol resulted in cells that expressed si

95 Mg(2+), Zn(2+), Cu(2+), K(1+), EDTA and beta-mercaptoethanol resulted in enhanced xylanase activ

96 as partially protected via co-treatment with beta-mercaptoethanol, resulting in reduced disulfide bon

97 Treatment of the Co3+-PEPCK complex with beta-mercaptoethanol results in a loss of cobalt and ful

98 Treatment of the complex with beta-mercaptoethanol results in near quantitative releas

99 In both instances, beta-mercaptoethanol reversed the inhibitory effects.

100 HIV-uninfected persons generated beta-mercaptoethanol-sensitive and -resistant antibodies

101 ed, and V(H)4 determinants were expressed by beta-mercaptoethanol-sensitive antibodies only; and HIV-

102 Moreover, human UBA3 could form a beta-mercaptoethanol-sensitive conjugate with NEDD8 in t

103 A beta-mercaptoethanol-sensitive Ubc9-sentrin conjugate co

104 Reduction with beta-mercaptoethanol showed that the 70-kDa protein cons

105 ol to 500 mM NaCl, 20 mM Tris (pH 8.4), 2 mM beta-mercaptoethanol significantly enhances dimer format

106 Skim milk with low levels of added beta-mercaptoethanol (SM-ME) and untreated skim milk (SM

107 At physiological pH, zinc and beta-mercaptoethanol stimulated the adenine DNA glycosyl

108 Inhibition by dithiothreitol and beta-mercaptoethanol suggests that intramolecular disulf

109 The active site contains a molecule of beta-mercaptoethanol that is positioned between His-106

110 from 280 to 100 kDa, but in the presence of beta-mercaptoethanol the top of the DSP smear disappeare

111 After selective elution with beta-mercaptoethanol, the peptides are sequenced using n

112 termediates in the reaction of L-serine with beta-mercaptoethanol, they retain activity in the reacti

113 The addition of the reducing agent beta-mercaptoethanol to samples prepared from METH-treat

114 The cross-linked complex was treated with beta-mercaptoethanol to transfer the 125I photomoiety fr

115 ould be incubated in 10 mM dithiothreitol or beta-mercaptoethanol until the precipitate is dissolved

116 hE(wt) stored in the presence of beta-mercaptoethanol was covalently modified at three cy

117 ion of the isoindole derivative L-serine-NDA-beta-mercaptoethanol was found to follow pseudo-first or

118 Va, derived from factor V treated with 1 mm beta-mercaptoethanol was inactivated more rapidly than t

119 ide exchange that leads to the generation of beta-mercaptoethanol, which in turn decouples the conjug

120 at the radical abstracts hydrogen atoms from beta-mercaptoethanol with a bimolecular rate constant =

121 Replacing beta-mercaptoethanol with dithiothreitol in the loading