ライフサイエンスコーパス: bicistronic

1 coding region, indicating that the sgRNA7 is bicistronic.

2 , transcripts encoding RRV R15 and ORF74 are bicistronic.

3 The second transcript, rhis4l, is bicistronic.

4 er synthesized by coupled translation from a bicistronic (-1 frameshift) clone, or reconstituted from

5 on of the gI and gE genes formed an abundant bicistronic 3.5-kb mRNA, as well as an abundant 2.0-kb g

6 In this study we use bicistronic 4get IL-4 reporter mice to directly visualiz

7 d AAT-opt transgenes separately, or a single bicistronic AAV vector.

8 Using bicistronic ACT1-LUC constructs, elongating yeast riboso

9 We now report the use of a bicistronic adenoviral vector (Ad-CMV-D2R80a-IRES-HSV1-s

10 The IRES-based bicistronic adenoviral vector can potentially be used in

11 To address this issue, we have constructed a bicistronic adenoviral vector encoding the two proteins

12 We report here the construction of a single bicistronic adenoviral vector for tumor-specific Bax exp

13 mize potential toxicity of the TRAIL gene, a bicistronic adenoviral vector that expresses the green f

14 (F) protein, using plasmid cotransfection or bicistronic adenoviral vectors, the retargeted H protein

15 We constructed a bicistronic adenovirus bearing BDNF under cytomegaloviru

16 d-uPAR) or wild-type p16 cDNA (Ad-p16) and a bicistronic adenovirus construct in which both the uPAR

17 oth E1A and E1B genes under the control of a bicistronic AFP-E1A-IRES-E1B cassette yielded improvemen

18 The remaining four transcripts are bicistronic and encode a series of novel truncated isofo

19 n papillomavirus 16 (HPV16) E6E7 pre-mRNA is bicistronic and has an intron in the E6 coding region wi

20 genesis (5 or 7 amino acid residues) in both bicistronic and monocistronic HCV cell culture-based (HC

21 Using both bicistronic and monocistronic mRNAs containing the c- my

22 eening of KSHV 3'UTRs has identified several bicistronic and polycistronic transcripts as the novel t

23 extended 3'UTRs of the 5' proximal genes of bicistronic and polycistronic transcripts offer addition

24 transcripts and 3'UTRs has revealed abundant bicistronic and polycistronic transcripts.

25 otide resolution of RNA-Seq data revealed 15 bicistronic and tricistronic messages along the genome,

26 Moreover, we detected numerous bicistronic and tricistronic mRNA messages in the ascovi

27 s.c. injections of the bicistronic antisense construct into established tumors

28 Subcutaneous injections of bicistronic antisense constructs into established tumors

29 ls the existence of a functionally important bicistronic antiviral mRNA and suggests a widespread rol

30 y combining bidirectional promoter (BDP) and bicistronic approaches to drive coordinated expression o

31 hat Aep3p, previously shown to stabilize the bicistronic ATP8-ATP6 mRNA and facilitate initiation of

32 ote F(1) assembly, have normal levels of the bicistronic ATP8/ATP6 mRNAs but fail to synthesize Atp6p

33 A bicistronic BVDV (cp strain NADL) was created that expre

34 Of note, bicistronic cadBA is transcribed at low constitutive lev

35 einitiation occurs efficiently on subgenomic bicistronic calicivirus mRNAs, enabling synthesis of min

36 nstituted this process in vitro on two model bicistronic calicivirus mRNAs.

37 FP TRE to control an artificial E1A-IRES-E1B bicistronic cassette in an adenovirus 5 vector (Ad5) and

38 ce ganciclovir (GCV)-resistant colonies in a bicistronic cellular reporter system with HCV internal r

39 and demonstrate that for tumor suppression, bicistronic coexpression of p14ARF and p53 is superior t

40 The effect with bicistronic construct (Ad-uPAR-Cath B) was much higher t

41 The bicistronic construct (Ad-uPAR-uPA) infected glioblastom

42 This bicistronic construct (pUM) inhibited the formation of c

43 of UAS-regulated responder genes in a single bicistronic construct also appeared to accelerate silenc

44 e nucleoside analogue ganciclovir by using a bicistronic construct coexpressing the herpes simplex vi

45 roduced into HeLa cells, stably expressing a bicistronic construct encoding the hygromycin B phosphot

46 ted ex vivo with the Ad-uPAR-MMP-9 antisense bicistronic construct showed decreased invasiveness and

47 f SNB19 cells with the Ad-uPAR-uPA antisense bicistronic construct showed inhibited invasiveness and

48 nsfection with Ad-gp91ds-eGFP (an adenoviral bicistronic construct targeting NAD(P)H oxidase in fibro

49 (TVP1), were linked via IRES to generate the bicistronic construct TNHXS1-IRES-TVP1.

50 n-tagged (HA-GlpF) polypeptides encoded by a bicistronic construct were expressed in bacteria.

51 /JFH (2a core), and H77/JFH (1a core), and a bicistronic construct, Bi-H77/JFH, all expressed both p8

52 plates that included the cSHMT 3'-UTR in the bicistronic construct.

53 ent protein by adenoviral gene transfer of a bicistronic construct.

54 potential problem further using promoterless bicistronic constructs coupled with RNase protection ass

55 Using bicistronic constructs for expression in K562 cells, we

56 By contrast, mice immunized with bicistronic constructs induced equivalent or higher leve

57 Use of bicistronic constructs revealed that TZD induction of IG

58 Interestingly, the presence of MV-F in the bicistronic constructs stimulated serum MV-specific immu

59 greater PRN titers than mice immunized with bicistronic constructs.

60 of the Y2441C substitution into the NADL or bicistronic cp viruses reconstituted the ncp phenotype.

61 We have generated a bicistronic Cre/LoxP reporter mouse line that pairs the

62 erfecta type II results from mutation of the bicistronic dentine sialophosphoprotein gene (DSPP ), we

63 findings, i.m. injection of the gp120/GM-CSF bicistronic DNA vaccine evoked a more extensive cellular

64 Thus, a bicistronic DNA vaccine that expresses gp120 and a trunc

65 A 100-fold lower dose of the gp120/GM-CSF bicistronic DNA vaccine was required to elicit detectabl

66 These results demonstrate that bicistronic DNA vaccines containing GM-CSF elicit remark

67 RNA molecule targeting the E7 region of the bicistronic E6 and E7 mRNA to induce RNA interference, t

68 y preventing translation initiation from the bicistronic eco29kIR-eco29kIM mRNA and causing its degra

69 -galactosidase (vector CT26), and the other (bicistronic) encodes eGFP and neomycin phosphotransferas

70 The S1 gene is bicistronic, encoding both the viral attachment protein

71 The S1 gene is bicistronic, encoding the viral attachment protein sigma

72 f a novel CXCR3 internal ribosome entry site bicistronic enhanced GFP reporter (CIBER) mouse in which

73 Bicistronic expression of green fluorescent protein (GFP

74 Human XIAP was delivered with bicistronic expression of green fluorescent protein (GFP

75 -subunit properties, we favored the use of a bicistronic expression plasmid encoding both GFP and a b

76 ternate mechanisms, we generated a series of bicistronic expression plasmids.

77 In addition, we use a bicistronic expression system that ensures recovery of s

78 We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle

79 Thus, this rare mammalian bicistronic gene coded for two tightly interacting brain

80 Here we have constructed a bicistronic gene containing both green fluorescent prote

81 iption of the major cold shock protein (CSP) bicistronic gene cspA1/A2 to increase by up to 300-fold.

82 labeled brain neurons in situ, indicative of bicistronic gene expression.

83 cleotide repeat within exon 47 of CACNA1A, a bicistronic gene that encodes alpha1A, a P/Q-type calciu

84 Our combination of shRNA and TuD in a single bicistronic gene transfer vector derived from Adeno-asso

85 onstrate that the downstream cistron, in the bicistronic gene, is expressed to a much higher level wh

86 mRNA expression of CTRP5 and MFRP, which are bicistronic genes, was studied by semiquantitative RT-PC

87 was involved in morphogenesis by creating a bicistronic genome in which NS2-3 was restored in the se

88 osome entry site between the two proteins (a bicistronic genome).

89 greatly diminished in mice vaccinated with a bicistronic gp120-Tat DNA vaccine, compared with those i

90 ated Tat protein before vaccination with the bicistronic gp120-Tat DNA vaccine.

91 he major transcript bearing GCR sequences is bicistronic, harboring coding sequences for another vira

92 rein, we describe for the first time a novel bicistronic HC-Ad driving constitutive expression of her

93 efficacy with no overt toxicities using this bicistronic HC-Ad even in the presence of systemic Ad im

94 f the efficacy, safety, and toxicity of this bicistronic HC-Ad vector in an animal model of GBM stron

95 The bicistronic HC-Ad-TK/TetOn-Flt3L was delivered into intr

96 Using a bicistronic HCV replicon system expressing a luciferase

97 lacking paralog group 13 expression using a bicistronic HOXA13/EGFP retroviral vector.

98 izing human liver (HLM), rat liver (RLM) and bicistronic human-cyt P450 3A4 (bicis.-3A4) microsomes a

99 In the present study we used bicistronic IFN-gamma-enhanced yellow fluorescent protei

100 on, which overlaps with the promoter for the bicistronic K14/v-GPCR delayed early gene that is transc

101 at the K6a-specific siRNA strongly inhibited bicistronic K6a-luciferase gene expression in vivo.

102 However, the novel bicistronic K8/K8.1 mRNAs translated a little K8 and no

103 atency protein coexpressed with vFLIP from a bicistronic latency-specific mRNA, was found to prevent

104 Examination of the more abundant bicistronic latent RNAs revealed the presence of an effi

105 high-efficiency transduction of HUVECs with bicistronic lentiviral vector encoding Noggin and enhanc

106 r successful hiPSC induction, we generated 4 bicistronic lentiviral vectors encoding the 4 RFs co-exp

107 he Tax oncoprotein of HTLV on hematopoiesis, bicistronic lentiviral vectors were constructed encoding

108 Erp (OspE/F-related) proteins from mono-and bicistronic loci that are carried on up to 10 separate p

109 hat the organization of these two genes in a bicistronic locus has been selected during evolution to

110 dent VEGF mRNA translation, as assessed by a bicistronic luciferase reporter assay.

111 dependent mRNA translation, as assessed by a bicistronic luciferase reporter assay.

112 nternal ribosome entry site activity using a bicistronic luciferase reporter plasmid, but none was de

113 We confirmed this finding by generating bicistronic luciferase reporter plasmids, and we were ab

114 ndependent translation were assessed using a bicistronic luciferase reporter transfected into neurons

115 d a longer, 4.2-kb transcript representing a bicistronic message derived from pagA and pagR.

116 The retrovirus generates a bicistronic message encoding the gene of interest and GF

117 n elongation factor 1alpha promoter drives a bicistronic message encoding the genes for human NOSII a

118 Production of oncoprotein from a bicistronic message is accomplished in bitransgenic prog

119 eased the level of production of OrfA from a bicistronic message that also encodes Rev.

120 n GFP is expressed from an IRES element in a bicistronic message with Elk-1.

121 Expression of cwlD in the forespore is in a bicistronic message with the upstream gene ybaK.

122 tion, inhibition of ribosome scanning on the bicistronic message, through insertion of a synthetic st

123 ave tested its efficiency of expression in a bicistronic message.

124 cannot, such as short abortive transcripts, bicistronic messages and overlapping transcripts that di

125 acy of Cre-mediated recombination in several bicistronic messages having the encephalomyocarditis vir

126 The bicistronic microRNA (miRNA) locus miR-144/451 is highly

127 Mutational analysis of bicistronic minigenomes and recombinant EBOVs showed no

128 Transcription of overlapping genes in EBOV bicistronic minigenomes followed the stop-start mechanis

129 n plasmid-transfected cells, analyses of the bicistronic minireplicon containing the wild-type or mut

130 n proteins, MAVS and miniMAVS, from a single bicistronic mRNA and suggest that noncanonical translati

131 CACNA1A coordinates gene expression using a bicistronic mRNA bearing a cryptic internal ribosomal en

132 tiviral innate immunity, is expressed from a bicistronic mRNA encoding a second protein, miniMAVS.

133 hepatitis B virus (DHBV) pregenomic RNA is a bicistronic mRNA encoding the core and polymerase protei

134 A bicistronic mRNA expression system was used to demonstra

135 When this 5'-leader was placed in a bicistronic mRNA expression vector, it functioned as an

136 -mediated initiation were investigated using bicistronic mRNA expression vectors.

137 plate for reverse transcription and also the bicistronic mRNA for core and polymerase.

138 genome and the production of the full-length bicistronic mRNA from the 35S promoter.

139 ysis revealed that hlyBA were expressed as a bicistronic mRNA induced approximately 2.5-fold under lo

140 model in which translation of the ATP8/ATP6 bicistronic mRNA is coupled to the availability of F1 fo

141 pressed in PEL cell lines as a large spliced bicistronic mRNA of 3.2 kb that also encompasses the ups

142 litate translation of the second gene in the bicistronic mRNA system, this result suggested that the

143 IRES activity during in vitro translation of bicistronic mRNA templates, and in MCF-7 and HeLa cells

144 pped to the intercistronic region (ICR) of a bicistronic mRNA that we cloned from the MDV-transformed

145 rnal ribosome entry sequence (HCV IRES) in a bicistronic mRNA was increased relative to cap-dependent

146 Additionally, a low-abundance ycfX-cobB bicistronic mRNA was observed which could encode up to t

147 ynthetic pathway, are spe-cified by a single bicistronic mRNA with overlapping reading frames.

148 t translation from an internal position of a bicistronic mRNA, and unlike cap-driven translation, it

149 translated from the downstream position on a bicistronic mRNA, called the pregenomic RNA, through a p

150 0 produces the CinA and CinQ proteins from a bicistronic mRNA.

151 r, the speB-prsA operon was transcribed as a bicistronic mRNA.

152 lation of the second open reading frame in a bicistronic mRNA.

153 ading through the gene junction to produce a bicistronic mRNA.

154 16 (HPV-16) can be translated from a single, bicistronic mRNA.

155 1) Vpu and Env proteins are expressed from a bicistronic mRNA.

156 y site located in the intergenic region of a bicistronic mRNA.

157 the mitochondrial genome and translated from bicistronic mRNAs containing both reading frames.

158 The bicistronic mRNAs encoded two distinct proteins, one of

159 family of immediate-early genes, the mature bicistronic mRNAs have variable 5' leader sequences due

160 nant human adenoviruses were used to express bicistronic mRNAs in murine organs.

161 nant human adenoviruses were used to express bicistronic mRNAs in murine organs.

162 5' leader (designated 5L) from a variant of bicistronic mRNAs that encode the pp14 and RLORF9 protei

163 ith high sensitivity, direct transfection of bicistronic mRNAs, and insertion of an iron response ele

164 le and leads to production of two additional bicistronic mRNAs, K8/K8.1alpha and -beta.

165 and in MCF-7 and HeLa cells transfected with bicistronic mRNAs.

166 demonstrated by Northern blot analysis, and bicistronic N-U mRNA was also evident.

167 Confirmation of the bicistronic nature of CYP27 mRNA by epitope mapping of u

168 inserted into WASP- bone marrow cells with a bicistronic oncoretroviral vector also encoding green fl

169 l membrane protein that is translated from a bicistronic open reading frame encoded within subgenomic

170 ivergently transcribed from pigS-pmpABC is a bicistronic operon (blhA-orfY), which encodes a metallo-

171 A clone that contained a putative bicistronic operon containing two genes that encoded 20-

172 asmids of Agrobacterium tumefaciens encode a bicistronic operon designated virH, which encodes two pr

173 4b, gtcA appears to be the first member of a bicistronic operon which includes a gene with homology t

174 Gene bba24 is part of a bicistronic operon with bba25 that encodes the well-char

175 ospB is transcribed from a bicistronic operon with ospA, a known spirochete adhesio

176 s with homology to yadA and found three: the bicistronic operon yadBC (YPO1387 and YPO1388 of Y. pest

177 es encoding these proteins are arranged in a bicistronic operon, with the tbpB gene located upstream

178 cribed as monocistronic mRNA species or as a bicistronic operon.

179 We show that AbiE systems are encoded by bicistronic operons and function via a non-interacting (

180 stablished that relBE, relFG, and relJK form bicistronic operons that are cotranscribed and autoregul

181 Here, we create an optimized bicistronic optogenetic system using Arabidopsis thalian

182 nes that encode either MV-H or MV-F alone or bicistronic or fusion system vectors that encode both MV

183 ore splice genes and encodes many genes with bicistronic or polycistronic transcripts.

184 The small-size class of mRNAs included two bicistronic orf2/rev mRNAs and two rev-like mRNAs, consi

185 ORF56 was expressed at low abundance as a bicistronic ORF56/57 transcript that utilized the same i

186 The BDP system was then combined with a bicistronic organization of genes at both ends of the pr

187 Secretion was markedly lower with the bicistronic pHR-GM/CD vector (average, 225.7 pg/10(6) ce

188 ith the monocistronic pHR-CD80 vector or the bicistronic pHR-GM/CD vector became 75% to 95% CD80 posi

189 Bicistronic plasmid expression of the RPE65 Gly244Val mu

190 However, immunization with a bicistronic plasmid that coexpressed gp120 and GM-CSF un

191 A library of bicistronic plasmids encoding the enhanced blue and gree

192 Two bicistronic plasmids were constructed that encoded the p

193 viral oncoproteins, E6 and E7, from a single bicistronic pre-mRNA containing three exons and two intr

194 his report, we provide the evidence that the bicistronic pre-mRNA intron 1 contains three 5' splice s

195 express, respectively, a tricistronic and a bicistronic pre-mRNA, which undergo alternative splicing

196 viral oncogenes, are expressed from a single bicistronic pre-mRNA.

197 t partially overlaps the core (C) ORF on the bicistronic pregenomic RNA (pgRNA).

198 pment, directly activates transcription of a bicistronic primary transcript encoding miR-1-2 and 133a

199 ransient transfection assay performed with a bicistronic Renilla-HCV IRES-firefly luciferase reporter

200 destinies of telencephalic precursors using bicistronic replication defective retroviruses.

201 spreadsheets could be easily adapted to any bicistronic reporter assay system.

202 Bicistronic reporter assay systems have become a mainsta

203 he systematic analysis of data obtained from bicistronic reporter assay systems.

204 Using the bicistronic reporter assay, we examined whether morphine

205 PH2 5'-UTR was suggested by the conventional bicistronic reporter assay; however, further stringent e

206 evidence for cellular IRES activity rests on bicistronic reporter assays, the reliability of which ha

207 o-delivering a firefly luciferase/mutant K6a bicistronic reporter construct and mutant-specific siRNA

208 We find that in the context of a bicistronic reporter construct, HIV-1 gag IRES' activity

209 al tool used in these reports was the use of bicistronic reporter constructs in which the 5'-UTR was

210 By using bicistronic reporter constructs, we showed that the tran

211 the generation of a mouse model, in which a bicistronic reporter expressing a red fluorescent protei

212 roots, of transgenic seedlings carrying the bicistronic reporter gene construct.

213 This Foxp3 bicistronic reporter knockin mouse model should greatly

214 ed IL-17A enhanced green fluorescent protein bicistronic reporter mouse strains to analyze in situ pr

215 gation of the two IRESs residing in the same bicistronic reporter mRNA revealed functional synergism

216 and hsp70 also increased levels of a second bicistronic reporter of Httex1 expression, mKate2, and i

217 nction as an internal ribosome entry site in bicistronic reporter plasmids, and it requires the 5'-pr

218 rted expression of a downstream cistron in a bicistronic reporter system.

219 ition of its cognate binding site within the bicistronic reporter transcript.

220 Utilizing the bicistronic reporter transposon mini-Tn5 lacZ-tet/1, we

221 strons, RT-PCR to detect mRNA encoded by the bicistronic reporter with high sensitivity, direct trans

222 sertion of an iron response element into the bicistronic reporter.

223 rs and early erythroblasts with the use of a bicistronic retroviral system, and their effects on CFU-

224 om the SK-N-SH cell line and inserted into a bicistronic retroviral vector also encoding green fluore

225 The bicistronic retroviral vector construct allowed for coex

226 te its role in normal adult hematopoiesis, a bicistronic retroviral vector encoding GATA-2 and the gr

227 with a murine stem cell virus (MSCV)-based, bicistronic retroviral vector overexpressing PPCA and th

228 For this study we used a novel bicistronic retroviral vector that engineers expression

229 For this purpose, a bicistronic retroviral vector was engineered that effici

230 5a/b-deficient background was rescued with a bicistronic retrovirus encoding TEL/JAK2 and Stat5a.

231 at harbors the AHBA biosynthetic operon, the bicistronic RifA construct and the pccB and accA1 genes

232 ling activities, either coding region of the bicistronic RNA can rescue the deficiency phenotype.

233 CV IRES-mediated translation programmed by a bicistronic RNA in vivo.

234 In the presence of Rev, this exon of the bicistronic RNA is skipped in a fraction of the spliced

235 Bicistronic RNA reporter transfection assays did not val

236 es from human papillomavirus 16 (HPV16) E6E7 bicistronic RNA was found to be a potent siRNA that supp

237 Importantly, the E6E7 bicistronic RNA with a mutant BPS and inefficient splici

238 ines with separate lentiviruses expressing a bicistronic RNA with a selectable marker as the first op

239 t vGPCR-containing transcripts are K14/vGPCR bicistronic RNAs that are strongly induced during lytic

240 on of globin mRNA in either monocistronic or bicistronic RNAs.

241 secretion it can be incorporated in a novel bicistronic secretion system.

242 We describe bicistronic single-exon Tat (72-amino-acid Tat [Tat72])-

243 am open reading frame (ORF) of the imprinted bicistronic Snurf-Snrpn locus in the mouse.

244 y transcript, mouse tissues express only the bicistronic Snurf-Snrpn transcript.

245 beta-galactosidase or tauGFP, by virtue of a bicistronic strategy that leaves the coding region of th

246 he effect of ROS on HCV replication, using a bicistronic subgenomic RNA replicon and a genomic RNA th

247 report the construction and application of a bicistronic system, involving the internal ribosome entr

248 By constructing a bicistronic system, our data support that the mRNA encod

249 NA and regulates alternative splicing of the bicistronic tat/rev mRNA.

250 etion of CUGBP1 decreased IRES activity from bicistronic templates that included the cSHMT 3'-UTR in

251 OxyR-dependent tpx promoter resulting in the bicistronic tpx/rbp mRNA.

252 Northern analysis reveals expression of the bicistronic transcript and in vivo imaging of GFP and lu

253 t between two open-reading frames of a plant bicistronic transcript can mediate translation of the se

254 ealed that gene 4 is transcribed as a 5.2-kb bicistronic transcript of the late kinetic class.

255 e trxB mRNA was cotranscribed with lolA as a bicistronic transcript or was present as a monocistronic

256 meric gene (named BIO3-BIO1) also produces a bicistronic transcript potentially encoding separate mon

257 through the gene-end sequence to generate a bicistronic transcript was enhanced compared to the obse

258 log of the p8 subunit (Dmp8) is encoded in a bicistronic transcript with the homolog of the Swc6/p18(

259 s ORF59 was expressed in high abundance as a bicistronic transcript, with a short 5' UTR and a long 3

260 rotein and mtTFB1 are encoded on a bona fide bicistronic transcript.

261 otein that is produced along with SmN from a bicistronic transcript; polycistronic mRNAs therefore ar

262 In eukaryotic cells, bicistronic transcripts are not common, and in some case

263 ese myogenic microRNAs, which are encoded by bicistronic transcripts or are nestled within introns of

264 previously undescribed viral RNAs, including bicistronic transcripts predicted to encode E5 and L2 or

265 Moreover, ~40% of bicistronic transcripts showed negative correlation in t

266 on initiation has been exploited to generate bicistronic transcripts that function in animal cells.

267 Using transgenic mice containing VEGF-LacZ bicistronic transcripts, we found that inhibition of glo

268 e, tTA(off) activates the transcription of a bicistronic transgene encoding PDX1 and an enhanced gree

269 ly repress translation in mammalian cells, a bicistronic translational repression assay was developed

270 first two methionine codons in ORF3 and was bicistronic; two closely spaced methionine codons in dif

271 uestion by examining the expression of model bicistronic (v-cyclin/LUC) transcripts in which a lucife

272 mRNA was inserted between the two genes of a bicistronic vector and transfected into various cell lin

273 DR) type 1, is linked to ANP expression on a bicistronic vector and was coexpressed in the human kera

274 after subcloning SPGP cDNA into a retroviral bicistronic vector capable of expressing both SPGP and t

275 Expression of bicistronic vector constructs showed that the 5'-UTRs of

276 n high yields in Escherichia coli by using a bicistronic vector encoding the PTP1B tyrosine phosphata

277 cell at a fixed relative level via a single bicistronic vector led to robust, uniformly normal angio

278 These data represent the first report of a bicistronic vector platform driving the expression of tw

279 In fact, T cells engineered with a bicistronic vector that concurrently expresses catalase,

280 idues Asp-90 and Arg-269 were generated in a bicistronic vector that encodes a 6-histidine-tagged ham

281 and expression of P450 and P450R on a single bicistronic vector using an internal ribosomal entry sit

282 (with NADPH-P450 reductase expressed using a bicistronic vector) were used to determine kcat and Km v

283 -driven messenger RNA were detected with the bicistronic vector, which may account for its poor expre

284 boxylase and the VKORC1 cDNA constructs in a bicistronic vector.

285 AUG 184 permits internal ribosome entry in a bicistronic vector.

286 ession of marker genes was observed with the bicistronic vector.

287 GM-CSF) and CD80 in separate vectors or in a bicistronic vector.

288 roach by adenoviral-mediated delivery of the bicistronic vector.

289 The use of bicistronic vectors carrying internal ribosome entry sit

290 These findings support the use of SIRES bicistronic vectors for a better assessment of therapeut

291 Using these sequences, we prepared two bicistronic vectors for mammalian expression of a repres

292 dimers can be produced by co-expression from bicistronic vectors in bacteria and that the co-expressi

293 ern blotting, pulse labeling, and the use of bicistronic vectors showed increased cap-dependent trans

294 Single-gene vectors, bicistronic vectors, and multigene vectors expressing up

295 The bicistronic viral RNA, which is transcribed at the late

296 recently reported on intragenomic replicons, bicistronic viral transcripts expressing an authentic re

297 This bicistronic virus exhibited slightly slower growth kinet

298 A splice variant is bicistronic, with distinct but overlapping reading frame

299 mal deficiency in zebrafish that removes the bicistronic wnt8 locus.

300 The bicistronic xylAB mRNA was processed by cleavage within