ライフサイエンスコーパス: bilin

1 covalent attachment of linear tetrapyrroles (bilins).

2 higher-energy bands were from more isolated bilins.

3 coupling for the second excited state of the bilins.

4 ate exciton splitting between closely-spaced bilins.

5 hytochrome without the addition of exogenous bilins.

6 omains and has 7 potential binding sites for bilins.

7 excited state of one of the allophycocyanin bilins.

8 raethyl-7,13-dimethyl-10-thia-(21H,2 3H,24H)-bilin-1,19-dione (1), was synthesized from 8-(2-carboxye

9 eption: reversible photoisomerization of the bilin 15,16 double bond.

10 gion upstream of the PAS domain knot and the bilin A and B pyrrole rings.

11 m studies with purified proteins showed that bilin absence reduces the strength of alphabeta interact

12 ecombinant CpcT was used to perform in vitro bilin addition assays with apophycocyanin (CpcA/CpcB) an

13 No significant bilin addition took place in a similarly engineered E. c

14 No significant bilin addition took place in a similarly engineered E. c

15 far-red reversible and a second class whose bilin adducts are nonphotochromic.

16 classes of bilin lyase domains--those whose bilin adducts are red, far-red reversible and a second c

17 Bilin amides were also assembled with BV-type and phytob

18 d crystallographic models, revealed that the bilin and GAF domain dynamically transition via breakage

19 ds of phycocyanin 612 originated from paired bilins, and the two higher-energy bands were from more i

20 ygenic phototrophs, chlorophylls, hemes, and bilins are synthesized by a common branched pathway.

21 Using the bilins as naturally occurring reporter groups, phycocyan

22 gene network implicates a widespread use of bilins as retrograde signals in oxygenic photosynthetic

23 bunits, covalent modification of subunits by bilin attachment and formation of the primary assembly u

24 chromophore and the apoprotein that promote bilin attachment and photointerconversion between the sp

25 bsorption triggers photoisomerization of the bilin between the 15Z and 15E photostates.

26 the recombinant apoproteins were tested for bilin binding and phytochrome photoactivity.

27 t encodes a large protein with two potential bilin binding sites, were amplified, and the recombinant

28 identified a glutamate residue critical for bilin binding.

29 es were generated by changing the codons for bilin-binding cysteines to alanine residues.

30 three-dimensional solution structure of the bilin-binding domain as Pfr, using the cyanobacterial ph

31 he roles of conserved amino acids within the bilin-binding domain of Deinococcus radiodurans bacterio

32 d cysteine at different positions within the bilin-binding GAF domain (cGMP-specific phosphodiesteras

33 nserved tyrosine residue (Tyr176) within the bilin-binding GAF domain of the cyanobacterial phytochro

34 A 1.16-A resolution crystal structure of the bilin-binding pocket in the dark-adapted red light-absor

35 of phyB mutants was generated affecting the bilin-binding pocket that altered photochemistry, therma

36 key amino acids that form a solvent-shielded bilin-binding pocket, and reveals an unusually formed de

37 parental lipocalin, the naturally occurring bilin-binding protein (BBP).

38 ide unambiguous evidence that the N-terminal bilin-binding region of BphP also provides a dimerizatio

39 ond GAF domain, the tongue region, seals the bilin-binding site in the GAF1 domain from solvent acces

40 A system reconstituting bilin biosynthesis in Escherichia coli was modified to u

41 itution of bilin biosynthesis to investigate bilin biosynthesis in streptophyte algae.

42 analysis and heterologous reconstitution of bilin biosynthesis to investigate bilin biosynthesis in

43 pectroscopy supports the conclusion that its bilin chromophore adopts a similar conformation to the r

44 an N-terminal domain that covalently binds a bilin chromophore and a C-terminal region that transmits

45 isms depends on key interactions between the bilin chromophore and the apoprotein that promote bilin

46 numerous reversible interactions between the bilin chromophore and the associated polypeptide.

47 that underpin photochemistry of the coupled bilin chromophore and the ensuing conformational changes

48 at involves a basic E/Z isomerization of the bilin chromophore and, in certain cases, the breakage of

49 CRs utilize a basic E/Z isomerization of the bilin chromophore as the primary step in their photocycl

50 reveal diurnal regulation of phytochrome and bilin chromophore biosynthetic genes in Micromonas.

51 Phytochromes are photoreceptors with a bilin chromophore in which light triggers the conversion

52 Zinc blot analyses confirm that a bilin chromophore is covalently bound to the algal phyto

53 erved GAF domain Tyr residue, with which the bilin chromophore is intimately associated, performs a c

54 t in the red light-absorbing (Pr) state, the bilin chromophore of the Deinococcus radiodurans proteob

55 are widely distributed photoreceptors with a bilin chromophore that undergo a typical reversible phot

56 loits reversible light-driven changes in the bilin chromophore to initiate a variety of signaling cas

57 pling photoreversible isomerization of their bilin chromophore to various signaling cascades.

58 HO family are important for synthesizing the bilin chromophore used to assemble photochemically activ

59 pport photochemical isomerization of a bound bilin chromophore, a process that triggers a conformatio

60 rminal region that covalently binds a single bilin chromophore, followed by a carboxy-terminal dimeri

61 idic residue to stabilize protonation of the bilin chromophore.

62 eceptors that utilize a linear tetrapyrrole (bilin) chromophore covalently bound within a knotted PAS

63 n of a covalently-bound linear tetrapyrrole (bilin) chromophore located in a conserved photosensory c

64 development, using the linear tetrapyrrole (bilin) chromophore phytochromobilin (PPhiB).

65 Bilin chromophores and bilirubin are involved in relevan

66 CikA covalently bound bilin chromophores in vitro, even though it lacks the ex

67 0 and 2 g/L protein concentration with eight bilin chromophores.

68 he biliproteins, which have covalently bound bilin chromophores.

69 s using cysteine-linked linear tetrapyrrole (bilin) chromophores to regulate biological responses to

70 initiated by light-driven isomerization of a bilin cofactor, which triggers protein structural change

71 How changes in bilin conformation affect output by these photoreceptors

72 ontrolled by the phytochrome (Phy) family of bilin-containing photoreceptors that detect red and far-

73 mes (Phys) encompass a diverse collection of bilin-containing photoreceptors that help plants and mic

74 Phytochromes are a collection of bilin-containing photoreceptors that regulate a diverse

75 Phytochromes are a collection of bilin-containing photoreceptors that regulate numerous p

76 We propose that different directions of bilin D-ring rotation account for these distinct classes

77 ults suggest that phycocyanin instability in bilin-deletion mutants is a consequence of diversion of

78 Our discovery of a bilin-dependent nuclear gene network implicates a widesp

79 d tetrapyrrole metabolism in plastids due to bilin depletion.

80 lterations whereby photoisomerization of the bilin drives nanometer-scale movements within the Phy di

81 pes of PBs takes place on an allophycocyanin bilin emitting at 660 nm (APC(Q)(660)) with a molecular

82 phycoerythrin 545 were suggested to have one bilin in each monomeric (alphabeta) unit of the dimer (a

83 of the A and D pyrrole rings, sliding of the bilin in the GAF pocket, and the appearance of an extend

84 These include residues that fix the bilin in the pocket, coordinate the pyrrole water, and p

85 ecies that lack phytochromes, can synthesize bilins in both plastid and cytosol compartments.

86 ed BVR lines implicate a regulatory role for bilins in plastid development or, alternatively, reflect

87 The potential role of bilins in subunit structure and assembly is examined in

88 nlike wild-type PcyA, both mutants possess a bilin-interacting axial water molecule that is ejected f

89 methods combined with NMR data show that the bilin is fully protonated in the Pb and Pg states and th

90 When CpcSU was assayed for bilin lyase activity in vitro with phycocyanobilin (PCB)

91 for cyanobacterial phytochrome 2, possessed bilin lyase activity, revealing two distinct classes of

92 We also show that phyB's N-terminal bilin lyase domain (BLD) and PHY domain interact directl

93 a 130-180 amino acid motif that delimits the bilin lyase domain, a subdomain of the extended phytochr

94 d by pattern searches represents a bona fide bilin lyase domain.

95 of highly conserved charged residues within bilin lyase domains of nearly all members of the extende

96 activity, revealing two distinct classes of bilin lyase domains--those whose bilin adducts are red,

97 The CpeS bilin lyase ligated PEB at both Cys(82) and Cys(139) of

98 Therefore, CpcSU is the bilin lyase-responsible for attachment of PCB to Cys-82

99 Specific bilin lyases are hypothesized to catalyze each PEB ligat

100 lated from the others, and the remaining six bilins may be in pairs.

101 plore the role of the propionate moieties in bilin metabolism, we report the semisynthesis of mono- a

102 substitution experiments using semisynthetic bilin monoamides, which indicate that the propionate sid

103 Monomers were stable and had bilin optical spectra different from the alpha2beta2 dim

104 The bilin organization of three cryptomonad biliproteins (ph

105 tron transfers from ferredoxin to protonated bilin:PcyA complexes.

106 Linear tetrapyrroles (bilins) perform important antioxidant and light-harvesti

107 her, our data support a toggle model whereby bilin photoisomerization alters GAF/PHY domain interacti

108 s, SyA-Cph1 and SyB-Cph1 covalently bind the bilin phycocyanobilin via their cGMP phosphodiesterase/a

109 Whereas incorporation of the native bilin phytochromobilin into PhyB confers robust Pfr -->

110 astoris were able to convert biliverdin to a bilin pigment, which produced a native difference spectr

111 ding from the Phy-specific domain toward the bilin pocket.

112 on of the porphyrin-like conformation of the bilin precursor to a more extended conformation.

113 . radiodurans phytochrome, we show that this bilin preference was partially driven by the change in b

114 ion at the C(15)=C(16) methine bridge of the bilin prosthetic group.

115 The bilin prosthetic groups of the phytochrome photoreceptor

116 the biosynthesis of the linear tetrapyrrole (bilin) prosthetic groups of cyanobacterial phytochromes

117 ns with cysteine-linked linear tetrapyrrole (bilin) prosthetic groups.

118 anaerobic assay protocol, optically detected bilin-protein intermediates, produced during the PcyA ca

119 imensional structural results better clarify bilin/protein interactions and help explain how higher p

120 g state illuminated the intricate network of bilin/protein/water interactions and confirmed the proto

121 We previously showed that bound bilin radical intermediates could be detected by low tem

122 This interconversion occurs via semireduced bilin radical intermediates that are profoundly stabiliz

123 de chains in substrate discrimination by two bilin reductase families while further underscoring the

124 tase is a member of the ferredoxin-dependent bilin reductase family and catalyzes two vinyl reduction

125 e to this conversion: a heme oxygenase and a bilin reductase with discrete double-bond specificity.

126 d of the representative ferredoxin-dependent bilin reductase, phycocyanobilin:ferredoxin oxidoreducta

127 operon consisting of a heme oxygenase and a bilin reductase, these studies establish the feasibility

128 res requires members of ferredoxin-dependent bilin reductases (FDBRs).

129 ynthesized by different ferredoxin-dependent bilin reductases (FDBRs): PPhiB is synthesized by HY2, w

130 chlorophyll catabolite reductases, which are bilin reductases involved in chlorophyll catabolism in p

131 or phytochromobilin, genes encoding putative bilin reductases were identified in the genomes of vario

132 reduced subsequently by ferredoxin-dependent bilin reductases with different double-bond specificitie

133 investigations defined three new classes of bilin reductases with distinct substrate/product specifi

134 scale movements within the Phy dimer through bilin sliding, hairpin reconfiguration, and spine deform

135 We also discovered that the nature of the bilin strongly influences Pfr stability.

136 g substituents alters the positioning of the bilin substrate within the enzyme, profoundly influencin

137 P Phi B synthase has a high affinity for its bilin substrate, with a sub-micromolar K(m) for BV.

138 ctron-coupled proton transfers to protonated bilin substrates buried within the phycocyanobilin:ferre

139 involved greater fitness using more reduced bilins, such as phycocyanobilin, combined with a switch

140 utative binding domains for chlorophylls and bilins, suggesting these proteins may function as a rese

141 Phytochromes are photoreceptors using a bilin tetrapyrrole as chromophore, which switch in canon

142 e light-driven conformational changes in the bilin to altered contacts between the adjacent output do

143 Propagation of the light signal from the bilin to the output module likely depends on the dimeriz

144 es for efficient energy migration, and other bilins transfer energy to this pair, extending the wavel

145 Our studies also suggest that bilins trigger critical metabolic pathways to detoxify m

146 heme and heme-derived linear tetrapyrroles (bilins), two critical metabolites respectively required

147 Phyllobilins, tetrapyrrolic, bilin-type chlorophyll degradation products, are abundan

148 ophore that can potentially be produced upon bilin uptake by any living cell expressing an apophytoch

149 The resulting bilin was incorporated into model cyanobacterial photore

150 nin 612, a major surprise was that a pair of bilins was apparently not found across the monomer-monom

151 monomer-monomer interface, but the remaining bilins were distributed as in the other two cryptomonad

152 Attachment of the central bilin, which is common to all biliprotein subunits, may

153 iven rotation within the covalently attached bilin, which then triggers a series of protein conformat

154 A hypothesis is that the coupled pair of bilins within the monomeric units offers important advan

155 The paired bilins within the protein monomers contained the lowest-