ライフサイエンスコーパス: binding affinity

1 orphism (which is implicated in differential binding affinity).

2 ing biophysical measurements of in vitro DNA-binding affinity.

3 hetic alpha(1-6)mannans gain better lectin's binding affinity.

4 thrombin and required the modifications for binding affinity.

5 by selection for SHMs that increase antigen-binding affinity.

6 ce, but also could be applied to measure the binding affinity.

7 inase kinetic activity and allosteric ligand binding affinity.

8 to accurately optimize protein stability and binding affinity.

9 shown to cause a 1000-fold increase in actin-binding affinity.

10 made by the lipid of Wnt to the Wnt-Frizzled binding affinity.

11 which it takes a long time to reset its DNA-binding affinity.

12 ia through specific iron chelators with high binding affinity.

13 ormation, and so on, without loss of antigen-binding affinity.

14 ycosylation and reduced extracellular ligand-binding affinity.

15 or variants with significantly increased ABA binding affinity.

16 stically to decrease both HbA and HbS oxygen binding affinity.

17 with CPX, was used, which showed 7 folds low binding affinity.

18 direct contributions of contact residues to binding affinity.

19 mechanism to transiently tune BAZ2B histone-binding affinity.

20 ase (GEN) activities of FEN1 but retains DNA-binding affinity.

21 quence and shape properties in predicting TF binding affinity.

22 sign of the surrounding residues to optimize binding affinity.

23 improves our ability to predict protein-DNA binding affinity.

24 8Q as a secondary mutation further increases binding affinity.

25 ne albumin-based therapeutic effects by FcRn-binding affinity.

26 proportional to FcRn expression and albumin-binding affinity.

27 ition of a 5'-triphosphate slightly enhances binding affinity.

28 wed a strong correlation with the changes in binding affinity.

29 interactions, affecting selectivity and drug-binding affinity.

30 uld be shown to yield significantly enhanced binding affinities.

31 e-activity relationship for predicting their binding affinities.

32 site mediated by only slight differences in binding affinities.

33 o calculate the magnitude of protein-protein binding affinities.

34 1129) matched for isotype and pre-F protein binding affinities.

35 es (nt), respectively, bind NS5 with similar binding affinities.

36 ns in protein coding regions on nucleic acid binding affinities.

37 as it involves the optimization of multiple binding affinities.

38 tic side-chains fine-tunes sequence specific binding affinities.

39 Some key compounds displayed high CB1R binding affinities.

40 o compute thermodynamic information, such as binding affinities.

41 ve mechanism of target binding on its Ca(2+) binding affinities.

42 ed a phenylpyridazinone fragment with a weak binding affinity (1, Ki = 160 muM).

43 utant had a significant reduction in Galphaq binding affinity (10-fold increase in Ki compared with W

44 Testing its binding affinities against other bromodomain-containing

45 Unlike albumin with low FcRn-binding affinity, albumin with high FcRn-binding affinit

46 VP24 mutations that lead to reduced KPNA binding affinity also decrease IFN inhibition and shorte

47 or d-aldohexoses in water with submillimolar binding affinities and completely distinguished the thre

48 Based on the predicted binding affinities and drug-like properties, small molec

49 protein-protein complexes, their associated binding affinities and experimental conditions were obta

50 nship between loop dynamics and experimental binding affinities and found that a prevalence of high l

51 BP65, and collagen and also determined their binding affinities and functions in vitro Hsp47 and FKBP

52 talytic activity formed the basis of diverse binding affinities and kinase activities of PKCalpha for

53 , all orthologs share the unusually high DNA binding affinities and low catalytic rates.

54 Of importance, the predicted serotype-binding affinities and peptide-anchor motifs did not sig

55 gyNet) for the predictions of protein-ligand binding affinities and protein stability changes upon mu

56 n for two specific problems: protein-protein binding affinities and protein thermal stability.

57 efined geometries without compromising their binding affinities and specificities.

58 tations showed a general correlation between binding affinity and ability to facilitate translation.

59 The binding affinity and avidity of integrins can be regulat

60 QM/MM calculation of warhead to predict the binding affinity and binding kinetics.

61 aused by the non-linear relationship between binding affinity and binding probability and the fact th

62 er indicated that PPTases possess the higher binding affinity and catalytic efficiency toward their c

63 H-START interaction increase both PtdIns(4)P-binding affinity and ceramide transfer activity of a CER

64 e present NetMHCpan-4.0, a method trained on binding affinity and eluted ligand data leveraging the i

65 recent measurements of the intrinsic ligand-binding affinity and free energy of each integrin confor

66 ngements that lead to a decreased nucleotide binding affinity and incorporation rate.

67 dual-labeled PSMA inhibitors exhibited high binding affinity and PSMA-specific effective internaliza

68 mechanism by which Cmpd-15 modulates agonist binding affinity and signalling.

69 The two types of complexes have comparable binding affinity and specificity, as judged from binding

70 ntal conditions were obtained from different binding affinity and structural databases.

71 duce that ubiquinone-10 has both the highest binding affinity and the fastest binding rate.

72 xists between the extent of reduction in DNA-binding affinity and the severity of symptoms in XP pati

73 f CoRA/FI synergy unexpectedly depends on FI-binding affinity and the stoichiometry of chemokine rece

74 mited structure to activate PKR by enhancing binding affinity and thereby increasing the population o

75 of a series of FimH antagonists in terms of binding affinity and thermodynamics, we demonstrate that

76 r represents a promising concept to increase binding affinity and to fine-tune functional properties.

77 at She1 affects the ATPase rate, microtubule-binding affinity, and stepping behavior of dynein, and t

78 ency directly correlated with target tubulin binding affinity, and the reduction in differential func

79 This intricate modulation of binding affinity, and thus cooperativity, renders our ne

80 perativity is observed, while in another all binding affinities are enhanced by an order of magnitude

81 interactions with polar solvents that reduce binding affinity are attenuated to a significant extent

82 pectroscopies indicate that these changes in binding affinity are due to the formation of Ni-oxalate

83 para-position of phenylalanine enhanced the binding affinity as much as 10-fold.

84 s, notably palmitate with a similar order of binding affinity as tablysin-15, a known palmitate-bindi

85 e also significantly modulates DENV protease binding affinity, as revealed by engineering the binding

86 Both binding affinity assays and docking studies of the CYP12

87 asing N2 solubility and the thermodynamic N2 binding affinity at Fe(0)(N2)(P4N2).

88 Direct calculation of relative binding affinities between antibodies and antigens is a

89 erturbation (FEP) protocol and calculate the binding affinities between the gp120 envelope glycoprote

90 dies revealed no detectable difference in TF-binding affinity between ALT-836 and Df-ALT-836 in vitro

91 However, the strong picomolar binding affinity between GAS6 and AXL and the promiscuit

92 Binding affinity between proteins and NPs and the occurr

93 Of interest, the binding affinity between Sesn2 and AMPK upstream LKB1 is

94 n in early endosomes was independent of FcRn-binding affinity, but differences in FcRn-binding affini

95 e entire AT-076 scaffold is crucial for high binding affinity, but the binding mode is likely differe

96 l antibodies and quantifies antibody-antigen binding affinities by quantitative reverse transcription

97 duction of Fe(3+)- to Fe(2+)-heme, decreased binding affinity by >20-fold.

98 e, when coupled with an extraordinarily high binding affinity by 2 (41-nt, KD = 45 pM) and high speci

99 s further optimised to achieve low-nanomolar binding affinity by in silico redesign of the CDRH3 sequ

100 of water-mediated hydrogen bonds and reduce binding affinity by increasing the enthalpic cost and, t

101 red interdomain linker modulates the histone-binding affinity by interacting with the PHD domain.

102 a interpretation is the determination of the binding affinity by isotherms of weight-average sediment

103 ides exhibit increased structural stability, binding affinity, cell permeability, and inhibition of R

104 The mutant showed higher binding affinity compared to native omega.

105 high-energy state displayed improved ligand binding affinity, compared with the wild-type enzyme, de

106 ghest affinity, with all variants decreasing binding affinity considerably.

107 primarily Vmax The extent of activation and binding affinity correlate with the number of phosphate

108 interactions have primarily been trained on binding affinity data.

109 aterally or longitudinally and whether Tau's binding affinity depends on the nucleotide state of tubu

110 1, but the affinity varied considerably, and binding affinity did not correlate with Dectin-1 agonism

111 Although their binding affinities differed only by a factor of 17, the

112 dopt different conformations affecting their binding affinity, DNA incorporation, and excision rates.

113 ctivation may stem from differences in IFN-1-binding affinity, duration of binding, number of surface

114 L-1RAcP heterodimerization and predicted the binding affinity during a complete thermodynamic cycle t

115 nd evaluated for EphA2 internalization rate, binding affinity, epitope binding, and hydrophobicity.

116 ubpockets was probed by calculating relative binding affinities for 23 adenine derivatives, resulting

117 action, as well as experimentally determined binding affinities for a selection of Wnt-Frizzled CRD i

118 his interaction nor any data regarding their binding affinities for each other.

119 it is an unusual enzyme in that it exhibits binding affinities for its substrate DNA and catalytic r

120 ered differences, which can illustrate co-TF binding affinities for motif analysis.

121 ggests that the model can accurately predict binding affinity for 75% of cases and that the error ass

122 exhibited approximately 10- to 20-fold lower binding affinity for AE and a reduced ability to be bloc

123 the interfacial hot-spot that dominates the binding affinity for an anti-interleukin-23 (anti-IL-23)

124 The higher MW PA had greater binding affinity for both glutenins and gliadins than lo

125 oping strategies lead to larger increases in binding affinity for CB[8] than for CB[7], which we attr

126 ge, progressive increases in N-domain Ca(2+)-binding affinity for D145E but only small changes for th

127 genes (clfA, clfB, bbp, and sdrC), all with binding affinity for fibrin(ogen).

128 We measured binding affinity for four synbodies against VLPs from mu

129 -SFRP interactions, indicating trends in Wnt binding affinity for Frizzled and SFRP CRDs.

130 of all IgG subclasses indeed showed enhanced binding affinity for isolated FcgammaRIII isoforms, with

131 onal complex 1 strains had a slightly higher binding affinity for its ligand than ClfB from strains f

132 The genetic variant increases the binding affinity for Klumpfuss (Klu), a zinc finger tran

133 Remarkably, the binding affinity for oritavancin against drug-resistant

134 omography (PET) tracer AV-1451 exhibits high binding affinity for paired helical filament (PHF)-tau p

135 Computational prediction of binding affinity for reversible covalent inhibitors pres

136 acterized the neutralization efficiency, the binding affinity for sigma1, and the effect of the antib

137 sitions have been prepared and used to study binding affinity for the BCL2 i-motif DNA.

138 ary mechanism is endocrine disruption of the binding affinity for the estrogen receptor, but oxidativ

139 he first quantitative analysis of changes in binding affinity for the STAT1 protein, and several vari

140 tprints enabled us de novo to reconstruct TF binding affinities genetically, which support the high-q

141 With the advantages of small size, high binding affinity, good stability, lack of immunogenicity

142 ortance of individual residues in conferring binding affinity has been explored for residues within C

143 Whereas protein-ligand binding affinities have long-established prominence, bin

144 f 10 muM, there were many hits for JJC8-016, binding affinities in the range of its DAT affinity were

145 rimetric and fluorescence titrations yielded binding affinity in the low micromolar range.

146 all ligands, largely independent of receptor binding affinities, in line with the similar STAT6 activ

147 Receptor binding affinity, in vivo tumor uptake, and biodistribut

148 l hydrazone (NSAH), using virtual screening, binding affinity, inhibition, and cell toxicity assays.

149 Additionally, we find that LAT:Grb2 binding affinity is altered by membrane localization.

150 Binding affinity is greatly enhanced by residues flankin

151 Binding affinity is increased by up to 1500-fold compare

152 protein on its cytoplasmic surface, and its binding affinity is modulated by light.

153 n Escherichia coli, had unprecedentedly high binding affinities (Kd approximately 10(-12) m) and high

154 s a fluorescently labeled substrate) and the binding affinity (KD ) indicate that, in both mutants, C

155 clic peptide-peptoid hybrids with high CXCR7 binding affinities (Ki < 100 nM) and measurable passive

156 (6) M(-1) min(-1)), endowed with a picomolar binding affinity (Ki = 38 pM), coupled with a single-dig

157 ods, the accurate characterization of TF-DNA binding affinity landscape still remains a challenging p

158 proach for modeling the transcription factor binding affinity landscape.

159 metal-induced alterations decrease RcnR-DNA binding affinity, leading to rcnAB expression.

160 In summary, the excellent binding affinity, low detection limit, high sensitivity,

161 The measured increase in DNA-binding affinity matches the calculated affinity change

162 vides chemical information to protein/ligand binding affinity measurements.

163 methods in the prediction of protein-ligand binding affinities, mutation induced globular protein fo

164 be at play, which explains the disparity in binding affinities observed for these systems.

165 In addition, the binding affinity obtained from the QCM-P device for anti

166 We calculated and compared the binding affinities of 20 carefully selected genes with t

167 atidyl inositol at least in part through the binding affinities of conserved N- and C-terminal domain

168 The measured relative binding affinities of dozens of human RNA binding protei

169 kinase Four-jointed (Fj), which affects the binding affinities of Ft and Ds.

170 re, we present crystal structures and ligand binding affinities of periplasmic binding proteins from

171 ctured gold have been connected to increased binding affinities of reactant molecules for low-coordin

172 Differences in the binding affinities of strains for substrates such as muc

173 The model was applied to predict the binding affinities of the full range of mouse and human

174 3-49), which binds to apoCaM or holoCaM with binding affinities of the same order of magnitude.

175 rofiles that make it possible to measure the binding affinities of these inhibitors for the enzyme.

176 ngineered to mimic these states modulate the binding affinities of Vps26 for a retromer cargo, result

177 on resonance showed a NOTA-conjugated ligand binding affinity of 1 nM.

178 n was here undertaken to further improve the binding affinity of 3 while preserving its selectivity a

179 plex containing the CC mismatch reveal a DNA binding affinity of 3.1 x 10(6) M(-1), similar to that o

180 titive GluN2 antagonists, of which ST3 has a binding affinity of 52 nM at GluN1/2A and 782 nM at GluN

181 The identified ligand had a binding affinity of 6.7 +/- 1.7 nM for PSMA and an excep

182 e three different regions, giving an overall binding affinity of 7.4 muM.

183 Improving the binding affinity of a chemical series by systematically

184 Our data suggest that the binding affinity of ACK relies on several conserved resi

185 l excretion kinetic and intrahepatic albumin binding affinity of CyG can further be used to different

186 mate these components using the relative MHC binding affinity of each neoantigen to its wild type and

187 ultured cells, the mutations reduced the DNA binding affinity of FOXI1, which hence, failed to adequa

188 In our model, the binding affinity of Ft and Ds depends on phosphorylation

189 The A-44G change markedly decreases the binding affinity of HuR, resulting in a moderate increas

190 ragine(N)297-linked glycans can modulate the binding affinity of IgG1 Fc to Fc gamma receptors, but i

191 ication is thought to only ripen the antigen-binding affinity of Igs that already exist (i.e., cognat

192 We could not measure the binding affinity of IL-36R to IL-1RAcP directly, so we e

193 eracts with IL-37, and potently enhances the binding affinity of IL-37 to the ALK1 receptor complex,

194 This is underpinned by the binding affinity of Kapbeta1 to phenylalanine-glycine nu

195 identified allosteric mechanism in which the binding affinity of LAT:Grb2 depends on the phosphorylat

196 Rx1 reduces the binding affinity of NbGlk1 for DNA in vitro.

197 ity relative to the T allele by reducing the binding affinity of PAX2.

198 In APOE4s a greater LDLR binding affinity of postprandial TRL after SFA, and lowe

199 s this goal but they focus on predicting the binding affinity of protein-ligand complexes, which is n

200 standing how gene-level mutations affect the binding affinity of protein-protein interactions is a ke

201 ination of the sedimentation coefficient and binding affinity of proteins in the micromolar range, th

202 This observation also explains improved binding affinity of the 5-E-VP -modified siRNA to human

203 The data reveal lower binding affinity of the BDBV VP24 (bVP24) for KPNAs and

204 515,Ala517/Ala515,Ala517)) with lower Ca(2+)-binding affinity of the C2C domain.

205 The binding affinity of the compounds with CYP121A1 was dete

206 antibody effector functions by affecting the binding affinity of the Fc to diverse Fc receptor family

207 fied nucleotide analogs demonstrate that the binding affinity of the incoming dNTP is controlled by t

208 The binding affinity of the ligand for PSMA and its internal

209 radiochemical yields and purity (>99%), and binding affinity of the modified antibody was confirmed

210 charged N terminus of histone H3, reduce the binding affinity of the PHD finger toward the histone su

211 d the platform to quantitatively measure the binding affinity of the prototypical RBP Vts1 for every

212 that, by tuning the stretching force and the binding affinity of the readers for chromatin, the fibre

213 ADP binding to the NBD alters the substrate-binding affinity of the SBD, triggering functionally ess

214 rostatic model that shows this change in DNA-binding affinity of these proteins can be fully explaine

215 Immunoprecipitation of isoform 2 validated a binding affinity of this isoform to both PI3K and BRAF/R

216 rrelation between predicted and experimental binding affinities on the training and test data after m

217 The strong dependence of inhibitor-binding affinity on both Mo oxidation state and inhibito

218 Binding affinity, on the other hand, can also be affecte

219 ing that genetic variation often affects the binding affinity only indirectly.

220 ng efforts to date have focused on improving binding affinities or biophysical characteristics.

221 trates are available and (ii) differences in binding affinities or substrate receptor abundances crea

222 at selective deuteration affected either the binding affinity or the activation by the selected ligan

223 ippocalcin did not affect stability, calcium-binding affinity or translocation to cellular membranes

224 dducts (OP-BChE) and BChE and provided equal binding affinity, permitting detection of the total enzy

225 gy have brought the opportunity for building binding affinity prediction methods, the accurate charac

226 ning methods as well as the state-of-the-art binding affinity prediction methods.

227 tion of these variants revealed impaired DNA binding affinity, reduced thermostability, diminished ex

228 The redox-modulated change in DNA-binding affinity regulates the ability of [4Fe4S] repair

229 conjugated ASOs showed 10-fold reduced ASGR binding affinity relative to three GalNAc ASOs but only

230 The 4 nM binding affinity requires phosphorylation at Rpb1 S1493

231 these two residues drastically affected DNA-binding affinity, resulting in rcnA expression in the ab

232 he effects of mutation on properties such as binding affinity, secondary and tertiary structure, conf

233 se ion-specific structural changes result in binding affinity shifts of up to sixfold, which happen t

234 Rn-binding affinity, but differences in FcRn-binding affinities significantly affected the albumin di

235 tems and found that both platforms show high binding affinity, specificity for the target antibody, a

236 terms of molecular energetics, photophysics, binding affinity, stability, and energy offsets with res

237 otides and show a direct correlation between binding affinity/stability and cellular activity.

238 the AztC flexible loop has no impact on zinc-binding affinity, stoichiometry, or protein structure, y

239 emonstrate the robustness of [(3)H]RO6957022 binding, affinity studies were carried out for a wide ra

240 those with rapid evolution of its predicted binding affinity, suggesting that all domains are necess

241 (18)F and evaluated its in vitro and in vivo binding affinity, targeting, and specificity.

242 , exhibited approximately 10-fold-lower KPNA binding affinity than either eVP24 or rVP24.

243 TEB had no more DNA binding affinity than Teb1 alone.

244 na RPA large subunit paralogs had higher DNA binding affinity than their large subunit alone.

245 roteins and identify a zone of optimal actin-binding affinity that allows for mechanical stress-induc

246 d mutants also showed reduced cocaine analog binding affinity that was not rescued by Zn(2+) Together

247 hough 1129 and 5C4 had similar pre-F protein binding affinities, the 5C4 neutralizing activity was ne

248 of the apoCaM-Ng13-49 is unknown due to low binding affinity, therefore we computationally generated

249 mutation reactivates P187S by enhancing FAD binding affinity through local and dynamic stabilization

250 alculate the impact of gene mutations on the binding affinity through the structure-based profiling o

251 ied a series of small molecules showing high binding affinities to BET proteins and low nanomolar pot

252 s discrepant residues resulting in different binding affinity to ABA.

253 o properties of an ideal RAF inhibitor: high-binding affinity to all RAF paralogs and maintenance of

254 soDGRFC, and CisoDGRC), which have different binding affinity to alphavbeta3 integrin, are reported f

255 BD2 provides a structural basis for its high binding affinity to BET proteins.

256 ational change, significantly increasing the binding affinity to BTE.

257 2 and IgG4 isotypes have significantly lower binding affinity to Fcgamma receptors.

258 arginine at position 435, which reduces its binding affinity to FcRn in vitro.

259 that a tight cavity can generate a stronger binding affinity to gas molecules than open metal sites.

260 Increasing the binding affinity to h-Syt II may improve botulinum neuro

261 The SEFL variants tested showed decreased binding affinity to human and cynomolgus FcgammaRs compa

262 ategy to engineer DNA structures with strong binding affinity to human serum albumin (HSA).

263 to create a CL7 tag, which retained the full binding affinity to Im7 but was inactivated as a DNase.

264 s of m4-1BB disclosed a very tight nanomolar binding affinity to m4-1BBL with an unexpectedly strong

265 ated FcgammaRIII isoforms, without affecting binding affinity to other FcgammaRs.

266 provide novel evidence that LIL3 shows high binding affinity to protochlorophyllide, the substrate o

267 oalloying Pt7 with boron modifies the alkene-binding affinity to reduce coking.

268 coli GusR's preferential DNA and glucuronide binding affinity to S. enterica GusR.

269 ations, the Vgll4-4A mutant possessed higher-binding affinity to TEAD1 than wild-type Vgll4.

270 rboring both TRF2 and POT1 has the strongest binding affinity to telomeric DNA substrates comprised o

271 Although RsmA exhibited the highest binding affinity to the cfcR transcript, single deletion

272 ega6) with mutant omega showed a decrease in binding affinity to the sigma-factors (sigma(70), sigma(

273 ne fragment was found to have low micromolar binding affinity to the zinc metalloenzyme carbonic anhy

274 f both mutations (HA190+HA212) increased the binding affinity to those glycans recognized by the HA19

275 Although Hop1 and Red1 exhibited similar binding affinities toward several DNA substrates, the tw

276 inct role for N-glycans in promoting MUC16's binding affinity toward galectin-3 and in causing retent

277 However, it has lower binding affinity toward the human version of its major r

278 accharides can exhibit dramatically improved binding affinity towards a protein.

279 Furthermore, the probe has 10-fold higher binding affinity towards Abeta aggregates (86nM) compare

280 ificity towards sialoglycans with millimolar binding affinity towards alpha2,3- or alpha2,6-sialyllac

281 iated vancomycin polymerization enhances the binding affinity towards these precursors.

282 e energy and the overall reversible covalent binding affinity using a two-state binding model.

283 gammaRIIIa-158F allotype displays a lower Fc binding affinity) using the TaqMan single nucleotide pol

284 ts significantly associated with altered VDR binding affinity (VDR-BVs) using a high-resolution (ChIP

285 The direction of the change in binding affinity was correctly predicted in a majority o

286 cRn-binding affinity, albumin with high FcRn-binding affinity was directed less to the lysosomes, sug

287 Accordingly, the protein-binding affinity was found to be slightly higher for 5-

288 ffinity to the alpha-interaction domain, but binding affinities were influenced by amino acid substit

289 Epitope binding affinities were mapped for human cross-reactive

290 Sub-micromolar binding affinities were obtained by fluorescence displac

291 There is very little loss of binding affinity when the CpG within the consensus bindi

292 als that IFNAR1 flexibility modulates ligand-binding affinity, which, in turn, regulates biological s

293 yanidin-3-O-glucoside had one of the highest binding affinities with all kinases, especially ABL1 (-8

294 preferred, following assessment of surrogate binding affinity with LRRK2 inhibitors.

295 ted membrane fusion and had higher levels of binding affinity with NHR peptide N46.

296 tide is derived from candoxin and shows high binding affinity with nicotinic acetylcholine receptors

297 ovides an example of the effect of E3 ligase binding affinity with relevance to other drug discovery

298 ) can influence protein structure stability, binding affinity with small molecules, and drug resistan

299 eolytic stability while maintaining receptor-binding affinities, with three candidate peptides retain

300 n in cell culture media corresponded to FcRn-binding affinity, with a approximately 3.3-fold increase