ライフサイエンスコーパス: biocatalyst

1 g., vaccines, drug delivery, imaging agents, biocatalysts).

2 IM lipase from Rhizomucor miehei was used as biocatalyst.

3 olerant, and effective cofactor-regenerating biocatalyst.

4 methane utilization in a promising bacterial biocatalyst.

5 amyl octanoate) using lipase Palatase as the biocatalyst.

6 ein is not the most efficient PHA polymerase biocatalyst.

7 aximize the benefit from this extremely fast biocatalyst.

8 h, external electron acceptor) into a single biocatalyst.

9 ure, and demonstrated its use as a promising biocatalyst.

10 RAG-1 lipase offers potential for use as a biocatalyst.

11 phatogenic strain of Escherichia coli as the biocatalyst.

12 ed using Candida antarctica lipase type B as biocatalyst.

13 ing that thermophiles have high potential as biocatalysts.

14 ynthesis of halogenated natural products, as biocatalysts.

15 tential to greatly enhance the robustness of biocatalysts.

16 PS-C1 (lipase from Burkholderia cepacia) as biocatalysts.

17 drug metabolism, and have potential uses as biocatalysts.

18 classic organic reactions in the presence of biocatalysts.

19 levance for optimization of flavin-dependent biocatalysts.

20 ctive circuits for biochemical processes and biocatalysts.

21 tion of self-regenerating microbial cells as biocatalysts.

22 development of new protein therapeutics and biocatalysts.

23 ctive methods for manipulating and tailoring biocatalysts.

24 their potential for generating truly de novo biocatalysts.

25 ion, which is often observed with whole cell biocatalysts.

26 ility of biomolecules as reusable and robust biocatalysts.

27 ted engineering for improvement of microbial biocatalysts.

28 for new compounds, novel enzymes and useful biocatalysts.

29 t that may be present in the active sites of biocatalysts.

30 r revolutionizing the preparation and use of biocatalysts.

31 reactions is rapidly reshaping our vision of biocatalysts.

32 coming the limitations of natural enzymes as biocatalysts.

33 tools for the manipulation and tailoring of biocatalysts.

34 for protein stability in the selectivity of biocatalysts.

35 he use of biologically derived materials and biocatalysts.

36 l for developing enzyme and whole cell based biocatalysts.

37 n and optimization of industrially important biocatalysts.

38 as being without peer as rationally designed biocatalysts.

39 of choice for the directed evolution of new biocatalysts.

40 bioreactors should be a rich source of novel biocatalysts.

41 ative to rational approaches for engineering biocatalysts.

42 mproved the availability of high-temperature biocatalysts.

43 istics that make them desirable as effective biocatalysts.

44 ntial wide application in industrial E. coli biocatalysts.

45 ed remarkable regio-promiscuity of nitration biocatalysts.

46 tion as possible sources of renewable energy biocatalysts.

47 ow cost, and self-replication and -repair of biocatalysts.

48 odologies were used in order to prepare four biocatalysts.

49 rformance of nicotinamide coenzyme-dependent biocatalysts.

50 itions were optimized as follows: whole-cell biocatalyst 0.8 g/L, leucine concentration 13.1 g/L, tem

51 cked bed reactor filled with the immobilized biocatalysts, 52.6 mmol of TCP was continuously converte

52 pproaches has produced many metal-containing biocatalysts able to adopt the functions of native enzym

53 may contribute to the dramatic increases in biocatalyst activity.

54 modification often requires large amounts of biocatalyst, adding significant costs to the process and

55 rapid identification of synthetically useful biocatalysts (along with their corresponding genes).

56 The engineered biocatalyst also reacts with a variety of indole analogu

57 se from Pseudomonas stutzeri was the fastest biocatalyst among all assayed, although poor discriminat

58 This platform was probed with uHTS for biocatalysts anchored to yeast with enrichment close to

59 st implementation of a nitrone synthase as a biocatalyst and establishes a novel platform for late-st

60 using Shewanella oneidensis MR-1 (MR-1) as a biocatalyst and performance was assessed in terms of cur

61 ansfer accelerator, alcohol dehydrogenase as biocatalyst and polydiallyldimethylammonium chloride as

62 ilitate the parallel evaluation of competing biocatalyst and process options.

63 tron transfer (DET) between a photosynthetic biocatalyst and the anode of a MFC.

64 ity, as well as enabling the design of novel biocatalysts and biosensors.

65 gh stereo-, regio-, and chemoselective redox biocatalysts and enabling reactions under mild condition

66 atalysis, potential untapped sources of such biocatalysts and how further optimization of these enzym

67 ct to the characteristics of the immobilized biocatalysts and synthesis conditions.

68 ymes before catalysis is a common feature of biocatalysts and that proteins have evolved under synerg

69 Activated biocatalysts and their novel regioselectivity difference

70 neering is industrially fruitful in creating biocatalysts and therapeutic proteins, applications of a

71 ctivity recovery, enzyme loading (wt% in the biocatalyst) and the physical properties, e.g. particle

72 materials for applications as biosensors and biocatalysts, and hold promise as bioactive, fouling-res

73 otein engineering, and medium engineering of biocatalysts are available, the main focus of this work.

74 Whole-cell biocatalysts are commonly limited by low tolerance of ex

75 increased production of novel extremophilic biocatalysts are discussed here.

76 Challenges related to the application of biocatalysts are discussed, including how 'green' a bioc

77 These biocatalysts are highly active (rates up to 400 min(-1))

78 ave been made available for this purpose, no biocatalysts are known to mediate this transformation.

79 laboratory-scale testing, as more efficient biocatalysts are needed.

80 ENGase biocatalysts are now finding burgeoning application for

81 Immobilized biocatalysts are suitable for removing TCP from contamin

82 the development of a surface display enzyme biocatalyst as an effective and renewable alternative fo

83 A tag is capable of operating as a secondary biocatalyst as well as bridging two enzymes for co-encap

84 this reason, the preparation of a library of biocatalysts as broad as possible may be a key turning p

85 neering enable the development of whole-cell biocatalysts as powerful tools in organic synthesis.

86 ers to facilitate identification of suitable biocatalysts, as well as references to recent volumes an

87 ve been readily catalyzed by the rudimentary biocatalysts available at an early stage in the origin o

88 ort a highly specific, convenient and robust biocatalyst based on a novel ether hydrolase enzyme, DNA

89 ndida antarctica lipase A (CAL-A) as a model biocatalyst based on a parameter study and fitting to th

90 A hydrogen evolution biocatalyst based on photosystem 1-platinum nanoparticle

91 ive of this study was to produce a composite biocatalyst, based on porous cellulosic material, produc

92 Cells with surface display are used as biocatalysts, biosorbents and biostimulants.

93 presents an opportunity to construct custom biocatalysts built in a lego-like fashion by inserting,

94 ges in oxidation state, iron is an excellent biocatalyst but also a potentially deleterious metal.

95 cterial communities provide a rich source of biocatalysts, but their experimental discovery by functi

96 as furnished a diverse array of halogenation biocatalysts, but thus far no examples of dehalogenating

97 In this study, we developed a new type of biocatalyst by immobilizing fungal laccase on the surfac

98 lop a new concept to impart new functions to biocatalysts by combining enzymes and metal-organic fram

99 Three general classes of modular biocatalysts can be identified: enzymes in which catalys

100 However, beyond these limitations, stable biocatalysts can be operated at higher temperatures or c

101 ed with detailed structural understanding of biocatalysts, can change the chemical industry from bein

102 phototroph, Rhodopseudomonas palustris, as a biocatalyst capable of light-driven CO2 reduction to CH4

103 The development of biocatalysts capable of fermenting xylose, a five-carbon

104 Biocatalysts capable of unlocking new and efficient Diel

105 prove the stability and recyclability of the biocatalyst compared to the free enzyme.

106 stereoselectivity of these cyclopropanation biocatalysts complements that of trans-(1S,2S)-selective

107 sign for continuous milk coagulation using a biocatalyst composed of immobilized animal and vegetable

108 ntinuous Feta-type cheese production using a biocatalyst consisting of immobilized rennin on a tubula

109 The enantiopreference of the biocatalyst could also be tuned to provide either enanti

110 The hydrolase based biocatalyst could provide the basis for a wide spectrum

111 ions, but widespread industrial use of these biocatalysts depends crucially on the development of new

112 g principles are used in the exploitation of biocatalysts derived from cells.

113 e approaches by which strain improvement and biocatalyst design are pursued in the future.

114 /d-xylose regulatory network is key for such biocatalyst development.

115 volution can be used in a routine manner for biocatalyst development.

116 Biocatalyst discovery and directed evolution are central

117 aminations with wild-type and Q240A variant biocatalysts displaying total turnover numbers of up to

118 evelopment and application of an immobilized biocatalyst, due to the well-known advantages over solub

119 Carbon nanomaterials regenerate the biocatalysts either by direct electron transfer or redox

120 iotransformations, these stereocomplementary biocatalysts enabled the multigram synthesis of the chir

121 Using toluene dioxygenase as biocatalyst, enantiopure cis-dihydrodiol and cis-tetrahy

122 in drug discovery, clinical diagnostics, and biocatalyst engineering.

123 new functions, drug metabolism, and in vitro biocatalyst engineering.

124 t library syntheses, process automation, and biocatalyst enhancements.

125 e the potential of TtHGXPRT as an industrial biocatalyst, enzymatic production of several dietary 5'-

126 of biomolecules including novel and improved biocatalysts (enzymes).

127 of traits was also constructed in succinate biocatalysts (Escherichia coli strain C derivatives) and

128 ver 70 functional OYE1 variants with several biocatalysts exhibiting over an order of magnitude impro

129 elopment of myoglobin-based cyclopropanation biocatalysts featuring dramatically enhanced thermostabi

130 mer methodology employing a Lewis acid and a biocatalyst, followed by nucleophilic substitution with

131 After the treatment of Muscat wine with the biocatalyst for 20days, free monoterpenes increased sign

132 onths with the possibility of storage of the biocatalyst for 30 d in wine at 25 degrees C.

133 erase was converted into the most proficient biocatalyst for [4+2] cycloadditions known.

134 ditional ethanologenic agent and a promising biocatalyst for advanced biofuels production using ligno

135 trocatalysts and Methanosarcina barkeri as a biocatalyst for CO2 fixation, we demonstrate robust and

136 H2-producing organism D. vulgaris is a good biocatalyst for converting formate to H2.

137 genesis and screening to afford a proficient biocatalyst for enantioselective cleavage and synthesis

138 essful de novo design of a single whole-cell biocatalyst for formal stereoselective C-H amination.

139 ggests that this enzyme could be a potential biocatalyst for industrial lipid hydrolysis and conversi

140 ese unique properties make CgNal a promising biocatalyst for industrial Neu5Ac biosynthesis.

141 nnin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clo

142 e phase transition of ELP and thus served as biocatalyst for OPs, while BSA was used to stabilize OPH

143 Laccase could act as a biocatalyst for oxygen reduction reaction along with cat

144 pendent ligation DNAzyme is implemented as a biocatalyst for the amplified detection of a target DNA

145 ducts, CloQ offers considerable promise as a biocatalyst for the chemoenzymatic synthesis of novel co

146 te the applicability of the BfrA-immobilised biocatalyst for the complete hydrolysis of concentrated

147 haromyces cerevisiae was used as the initial biocatalyst for the conversion of glucose into muconic a

148 the requirements for an industrial strength biocatalyst for the direct conversion of biomass to comb

149 ctrum of activities make MdP2'GT a promising biocatalyst for the industrial preparation of the corres

150 ed chemo-enzymatic pathway and an engineered biocatalyst for the multistep synthesis of an important

151 forming reverse reaction, making it a useful biocatalyst for the production of chiral alpha-hydroxy k

152 scope and limitations of using E. coli as a biocatalyst for the production of diesel-like fuels.

153 iven the unique nature of 29G12 as a protein biocatalyst for this chemical reaction, we have investig

154 as used to expand reaction scope, generating biocatalysts for amide bond formation from carboxylic ac

155 gn promises to accelerate the development of biocatalysts for applications in the pharmaceutical, che

156 spread application of omega-transaminases as biocatalysts for chiral amine synthesis has been hampere

157 el platform for development of gram-positive biocatalysts for conversion of lignocellulosic materials

158 The use of thermophilic microorganisms as biocatalysts for electromethanogenesis was investigated.

159 Exciting developments include new biocatalysts for enantioselective carbon-carbon bond for

160 a pastoris, are investigated and compared as biocatalysts for glucose oxidation using flow injection

161 e a promising strategy for creating tailored biocatalysts for many synthetically useful reactions.

162 importance of selecting from both chemo- and biocatalysts for optimal results.

163 eotide phosphate, and have become attractive biocatalysts for organic synthesis.

164 ure elaboration into efficient and selective biocatalysts for organosiloxane chemistry.

165 Oxygenases are promising biocatalysts for performing selective hydroxylations not

166 This study therefore unveiled new routes and biocatalysts for polyketide cyclization.

167 ader peptide, making them potentially useful biocatalysts for preparation of new thiopeptide variants

168 rk components may help in the development of biocatalysts for production of fuels and chemicals from

169 ese may have potential utility as industrial biocatalysts for production of maltose.

170 discussed in relation to the design of novel biocatalysts for production of therapeutic flavonoids.

171 specificity, it is now possible to optimize biocatalysts for specific applications.

172 tly flavoproteins, were shown to be powerful biocatalysts for synthetic organic chemistry application

173 nthetic prokaryotes showing great promise as biocatalysts for the direct conversion of CO2 into fuels

174 rable interest in the development of PALs as biocatalysts for the enantioselective synthesis of non-n

175 o- and di-oxygenases into practically useful biocatalysts for the hydroxylation of a wide range of ar

176 ly studied, and both were found as excellent biocatalysts for the production of adequate chiral inter

177 derivatives, and the potential of engineered biocatalysts for the production of quaternary centers.

178 abled the rapid evolution of three efficient biocatalysts for the selective hydroxylation of a primar

179 Genetic studies to obtain improved biocatalysts for the selective removal of sulfur and nit

180 zyme (OYE) family are widely used, effective biocatalysts for the stereoselective trans-hydrogenation

181 n vitro hydroxynitrile lyases are proficient biocatalysts for the stereospecific synthesis of cyanohy

182 in enzymes that can be harvested as powerful biocatalysts for the synthesis of both new drugs and exi

183 scaffolds and have considerable potential as biocatalysts for the synthesis of small molecule glycosi

184 m engineering, protein (enzyme) engineering, biocatalyst (formulation) engineering, biocatalytic casc

185 rements and hyperfine tensor measurements of biocatalyst formulations inhibited with 4-fluorobenzenes

186 Silicatein, an enzymatic biocatalyst from the marine sponge Tethya aurantia, is d

187 tained their ability to protect encapsulated biocatalysts from degradation by proteases.

188 n submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; stil

189 The synthetic effectiveness of these biocatalysts has been significantly increased by the pro

190 These engineered MAO-N biocatalysts have been applied in deracemization reactio

191 detoxification processes based on these live biocatalysts have been developed.

192 De novo biocatalysts have been successfully generated by computa

193 Biosensors and biocatalysts have limited applicability to the more trad

194 ble and robust construction of truly de novo biocatalysts holds promise for applications in chemical

195 ty of 22.2 g of substrate hydrolysed/gram of biocatalyst/hour.

196 Biocatalysts, however, are delicate materials that hover

197 he availability of efficient and inexpensive biocatalysts (i.e. alcohol dehydrogenases, cellulases an

198 oaches to enhancing the stability of protein biocatalysts: (i) rational design, based on knowledge of

199 ement scenarios targeting biocatalyst yield, biocatalyst immobilization for reuse, and elimination of

200 biocatalytic reaction may be, and trends in biocatalyst improvement through enzyme engineering are p

201 limonene into alpha-terpineol using the same biocatalyst in both processes, Fusarium oxysporum 152B.

202 usion, strongly affect the efficiency of the biocatalyst in each specific reaction system.

203 n strategy and implicates a potential unique biocatalyst in mccrearamycin cyclopentenone formation.

204 d is, therefore, of particular interest as a biocatalyst in synthetic organic chemistry.

205 The active biocatalyst in the anodic chamber was a mixed culture of

206 n, improved stability and the absence of the biocatalyst in the product stream.

207 eactions, are of great interest as potential biocatalysts in a number of applications.

208 e developments should help expand the use of biocatalysts in industry.

209 rom the sea have begun to emerge as powerful biocatalysts in medicinal chemistry and total synthesis.

210 outcome of reactions entice chemists to use biocatalysts in organic synthesis.

211 6-membered tylosin macrocycle, using them as biocatalysts in the chemoenzymatic synthesis of tylacton

212 The use of these biocatalysts, in tandem, could potentially find applicat

213 asonable step to improve the performances of biocatalysts (including both enzymes and whole-cell syst

214 m(ii) complexes as anticancer candidates and biocatalysts, including arene ruthenium complexes, polyp

215 key factors that determine the efficiency of biocatalysts, including surface area, mass transfer resi

216 The biocatalyst is capable of up to 1,300 turnovers, exhibit

217 2+)-dependent ligation DNAzyme as amplifying biocatalyst is presented.

218 actions not catalysed by naturally occurring biocatalysts is a challenge for protein engineering and

219 efficient, environment-friendly, commercial biocatalysts is highly attractive.

220 has been devoted toward improving enzymes as biocatalysts is presented.

221 ever, one limitation in the discovery of new biocatalysts is the screening or selection methods emplo

222 than nature" NCBs and several ene reductase biocatalysts is used to indicate transfer by quantum mec

223 roup into unprotected phenols by employing a biocatalyst (laccase), tBuOOH, and either the Langlois'

224 ous catalytic methods is presented, covering biocatalysts, Lewis acid catalysts based on boron and me

225 ntages in view of stability, volume specific biocatalyst loading, recyclability as well as simplified

226 Biocatalysts may be delicate proteins, however, once sta

227 ifferences in the performance of immobilised biocatalysts must be interpreted very carefully.

228 The NiN2S2 portion of the biocatalyst (N2S2 = a cysteine-glycine-cysteine or CGC4-

229 t low temperature (e.g., 50 degrees C) using biocatalysts of 6 commercial lipases adsorbed on hydroph

230 This carbon-silicon bond-forming biocatalyst offers an environmentally friendly and highl

231 Using a strain of Desulfuromonas as biocatalyst on the anode resulted in an acetate producti

232 inimal external risks, given that engineered biocatalysts only have improved fitness within the custo

233 The expanding "toolbox" of biocatalysts opens new opportunities to redesign synthet

234 bilization of enzymes aiming at their use as biocatalysts or biosensors.

235 evolution is the experimental improvement of biocatalysts or cellular properties through iterative ge

236 nd 1, 2 &4 in Escherichia coli affords three biocatalysts over-expressing 4-8 enzymes for one-pot con

237 nd/or enzymes have to be tailored to improve biocatalyst performance.

238 58 ns (98% CsF) as (k(cat)/K(M))(app) of the biocatalyst preparation increased from 0.092 s(-1) x M(-

239 ive DeltaDeltaS(dagger), for the more active biocatalyst preparations in organic solvents.

240 The best CALB biocatalyst preserved 90% of the activity after 30days u

241 With resistant biocatalysts, product yields (ethanol and succinate) fro

242 Biocatalyst production was highly correlated with cost a

243 The biocatalyst, referred to as surface display laccase (SDL

244 hree-dimensional structure of this important biocatalyst reveals that residues implicated in the enha

245 CP can influence a biocatalyst's function by altering protein backbone flex

246 extremely diverse, widespread, and versatile biocatalysts, sensors, and molecular transporters.

247 The resultant biocatalysts showed broad applicability toward the synth

248 By examining various biocatalyst/substrate combinations, it was demonstrated

249 a Daphnia magna model organism and a perlite biocatalyst support material demonstrate the attainable

250 r high surface area:volume ratios, nanoscale biocatalyst systems exhibit unique behaviors that distin

251 f motion for residual water molecules on the biocatalyst, (tau(c))(D(2)O), in hexane decreased from 6

252 OxaD is a versatile biocatalyst that converts an array of semisynthetic roqu

253 xygenase (Rubisco) is a globally significant biocatalyst that facilitates the removal and sequestrati

254 y, offers scope for the development of novel biocatalysts that are both highly active and robust.

255 tase metalloenzymes are unique and important biocatalysts that are used industrially to produce high

256 xtending the range of natural and engineered biocatalysts that can be customised for clean industrial

257 Central to this challenge is the design of biocatalysts that can efficiently convert cheap lignocel

258 or Nature's choice of ligand environments in biocatalysts that carry out olefin oxidations.

259 tion, there are increased efforts to develop biocatalysts that confer regioselectivity for site-speci

260 synthetic biology-and the robustness of the biocatalysts that convert the metabolic intermediates to

261 teins' stability to enhance their utility as biocatalysts, therapeutics, diagnostics and nanomaterial

262 an be a carrier of genetic information and a biocatalyst, there is a consensus that it emerged before

263 ligninases promises the development of novel biocatalysts, these enzymes have largely been characteri

264 we report the creation of improved nitration biocatalysts through constructing and characterizing fus

265 biological processes rely on highly complex biocatalysts, thus limiting their industrial application

266 ective oxidations, and represent a promising biocatalyst to address this challenge.

267 le MFC used Pseudomonas aeruginosa PAO1 as a biocatalyst to generate the maximum power and current de

268 PQQ serves as an efficient biocatalyst to mediate the oxidation of thiols at a subs

269 ive site makes XdINV a valuable and flexible biocatalyst to produce novel bioconjugates.

270 lum could potentially be used as a microbial biocatalyst to produce renewable fuels directly from lig

271 alcohol dehydrogenases were investigated as biocatalysts to enantioselective oxidation of racemic er

272 ase (IRED) and omega-transaminase (omega-TA) biocatalysts to establish the key stereocentres of these

273 e for catalysis, future work will tailor the biocatalysts to high-demand synthetic processes by evolv

274 f bioprocesses and the ability of engineered biocatalysts to produce designer products at high carbon

275 This use of different biocatalysts to select different C-H positions contrasts

276 n of local photoelectrocatalytic activity of biocatalysts towards light-induced hydrogen evolution.

277 re now being applied for the optimization of biocatalysts used in the production of a wide range of p

278 highlights the applicability of one class of biocatalysts viz."lipases" in synthetic transformations,

279 The criteria used to choose the appropriate biocatalyst was based on the time of coagulation in succ

280 The immobilized biocatalyst was characterized and then tested in fruit j

281 A new immobilized biocatalyst was developed using glyoxyl-agarose as suppo

282 The engineered biocatalyst was shown to be a thermostable, solvent tole

283 The composite biocatalyst was studied by Scanning Electron Microscopy

284 This biocatalyst was the result of three rounds of mutagenesi

285 removal using cell-free bacterial enzymes as biocatalysts was investigated using crude cell lysates a

286 Performance of the biocatalysts was tested for lactose hydrolysis, and the

287 with Myceliophthora thermophila laccase, as biocatalyst, was performed in aqueous medium using an ec

288 onene-1,2-diol and a loss of activity of the biocatalyst were observed after intense cell treatment,

289 omega-TA biocatalysts were also successfully employed for the pro

290 5h of reaction using 0.05g/mL of immobilized biocatalyst, which released 3mg/mL of reducing sugars an

291 design and directed evolution are versatile biocatalysts whose promiscuous activities represent pote

292 e a key turning point to find an immobilized biocatalyst with improved properties when compared to th

293 g and robust scaffold for the development of biocatalysts with carbene-transfer reactivity.

294 The platform complements biocatalysts with characteristics of heterogeneous catal

295 ubstrate specificity and to design effective biocatalysts with glycosylation and/or deglycosylation a

296 sight into engineering new cutinase-inspired biocatalysts with tailor-made properties.

297 cm(2) using Shewanella oneidensis as a model biocatalyst without any optimization of bacterial cultur

298 uld provide a convenient means for preparing biocatalysts without the need for enzyme extraction and

299 or better than previously reported for other biocatalysts (yeast and bacteria) requiring complex vita

300 Realistic improvement scenarios targeting biocatalyst yield, biocatalyst immobilization for reuse,