ライフサイエンスコーパス: biolistic

1 the Pioneer inbred PHH5G is recalcitrant to biolistic and Agrobacterium transformation.

2 iled protocols for two different procedures, biolistic and electroporation, which have been used to t

3 lant transformation methods ( Agrobacterium, biolistics and protoplast fusion), insertion of DNA into

4 -null dorsal root ganglion (DRG) neurons via biolistics and were compared with the same construct exp

5 A biolistic approach was used to integrate two fusion gene

6 We evaluated particle bombardment (biolistics) as a strategy to introduce and express nucle

7 delivery: the PEG-mediated delivery and the biolistic bombardment based delivery, either of these me

8 in which it is not present in nature, by the biolistic bombardment of cultured immature embryos.

9 In sink leaves, biolistic bombardment of plasmids encoding GFP-fusion pr

10 Biolistic delivery of guide RNAs (as RNA molecules) dire

11 Here we present an optimized protocol for biolistic delivery of plasmid DNA into the epidermis of

12 Here we report biolistic delivery of pre-assembled Cas9-gRNA ribonucleo

13 proteins from a single vector following its biolistic delivery or Agrobacterium-mediated genetic tra

14 yanine dyes, combined with particle-mediated biolistic delivery using a hand-held gene gun, allows no

15 Nuclear transformation methods include biolistic delivery, agitation with glass beads, and elec

16 Using the biolistic gene gun, cDNAs encoding a dominant negative m

17 Using the biolistic gene gun, cDNAs encoding a mutant Kv4.2 alpha

18 ced into a homozygous rye inbred line by the biolistic gene transfer.

19 Using the Biolistics Gene Gun, cDNA constructs encoding a Kv1 subf

20 Using the Biolistics Gene Gun, cDNA constructs encoding either Kv2

21 e plasmid vaccines were delivered to mice by biolistic (gene gun) intradermal inoculation.

22 ed food production through Agrobacterium and biolistic genetic modifications for the development of '

23 days without using expensive equipment (e.g. biolistic gun or electroporation apparatus) or complicat

24 r was bombarded into P. vittata spores using biolistics, in which putatively stable transgenic gameto

25 Biolistic inoculation of cantaloupe seedlings with the M

26 Efficient protocols were established for the biolistic introduction of a construct containing a repor

27 SAS was activated by biolistic introduction of promoterless constructs, indic

28 In this current study, a biolistic leaf transformation assay has provided direct

29 -Nia2 DNA by the gene transfer method called biolistics led to localised acquired silencing (LAS) in

30 Expression of Cre recombinase using biolistics, lentivirus or genetic intercrosses triggered

31 Biolistic loading of differentiated plant cells is easie

32 s, and these particles were delivered by the biolistic method to plant cells harboring loxP sites fla

33 and to reach the desired tissue through the biolistic method.

34 We used biolistic methods to transfect neurons with gold 'bullet

35 into plastids of tobacco leaf tissues, using biolistic microprojectile bombardment, in an effort to d

36 ypically achieved using Agrobacterium T-DNA, biolistics or by stably integrating nuclease-encoding ca

37 Biolistic overexpression of M-CSFR on microglia endogeno

38 oration or into Anopheles gambiae embryos by biolistic particle bombardment.

39 f epidermal cells transiently transformed by biolistic particle delivery were used to confirm and ref

40 ximodistal respecification, we have used the biolistics (particle bombardment) technique to transfect

41 t, which are introduced into plastids by the biolistic process.

42 adult schistosomes further suggest that the biolistic strategy should be applicable to a variety of

43 RK1- and GIRK2-expressing plasmids using the biolistic technique.

44 estigate mechanisms of localization, we used biolistic techniques to express GFP-tagged PSD-95 (PSD-9

45 To overcome these problems, we have adapted biolistic techniques to load Ca2+-sensitive dyes into gu

46 method for introducing dyes into cells using biolistic techniques.

47 Using biolistics to label single cells on acute brain slices o

48 We have used particle bombardment (biolistics) to deliver replication-competent wheat dwarf

49 verexpression of Bcl-2 by means of transient biolistic transfection experiments of HUVE cells was suf

50 Biolistic transfection is a technique in which subcellul

51 Using biolistic transfection of individual RGCs and multielect

52 We have used biolistic transfection of organotypic slices of P12 cere

53 Here we provide a detailed protocol for biolistic transfection of plasmids into cultured human e

54 Biolistic transfection will facilitate experimental stra

55 Using organotypic hippocampal slices and biolistic transfection, we found the acid-sensing ion ch

56 models, and particle-mediated gene transfer (biolistics) transfection methods to identify critical DN

57 he C. lusitaniae CLS12 gene was disrupted by biolistic transformation and homologous recombination.

58 Our results show that biolistic transformation can be used with transient assa

59 We have developed a technique for the biolistic transformation of Arabidopsis thaliana root ha

60 nit of the chloroplast F1-ATPase was made by biolistic transformation of Chlamydomonas reinhardtii.

61 B chromosomes are readily fractionated by biolistic transformation of truncating plasmids.

62 air templates into maize immature embryos by biolistic transformation targeting five different genomi

63 Using biolistic transformation, we disrupted one of the two co

64 e then directly transformed into maize using biolistic transformation.

65 n gene was introduced in soybean by standard biolistic transformation.

66 Biolistic transient expression of UBA2-GFPs demonstrated

67 Biolistics using a promoter reporter showed that GA and