ライフサイエンスコーパス: bioorthogonal

1 t in any cell, the protein folding sensor is bioorthogonal.

2 We present a bioorthogonal and modular conjugation method for efficie

3 yclooctene-tetrazine cycloadditions are both bioorthogonal and mutually orthogonal: trans-cyclooctene

4 nd find widespread use for different in vivo bioorthogonal applications, particularly in the biomedic

5 eptionally mild conditions and constitutes a bioorthogonal approach toward amidines by avoiding the u

6 In an effort to develop a bioorthogonal AS-kinase inhibitor and to extend this tec

7 nt in biological systems, making it a unique bioorthogonal atom for probing molecular interactions in

8 For this purpose, IONPs with bioorthogonal azide and alkyne surfaces masked by polyet

9 recognition motif that allows us to append a bioorthogonal azide or alkyne moiety and use the Cu(I)-c

10 hol-containing molecules and as reagents for bioorthogonal bond-cleavage reactions.

11 Recent advances in bioorthogonal catalysis are increasing the capacity of r

12 Bioorthogonal catalysis broadens the functional possibil

13 argeting concepts can be translated to rapid bioorthogonal chemical approaches with retained potentia

14 We tailored cell surfaces with bioorthogonal chemical groups on the basis of a liposome

15 The azide serves as a bioorthogonal chemical handle for selective modification

16 In this study, we have demonstrated bioorthogonal chemical imaging of DNA, RNA, protein and

17 tabolic GalNAz labeling of human cells and a bioorthogonal chemical probe to affinity-purify and iden

18 Fluorogenic probes activated by bioorthogonal chemical reactions can enable biomolecule

19 Bioorthogonal chemical reactions, those that do not inte

20 t thiopeptide variants containing ncAAs with bioorthogonal chemical reactivity can be further postbio

21 Using a bioorthogonal chemical reporter and labeling strategy to

22 Metabolic labeling of glycans with a bioorthogonal chemical reporter such as the azide enable

23 complish this goal, we took advantage of the bioorthogonal chemical reporter technique.

24 The bioorthogonal chemical reporters described here should s

25 Here we report bioorthogonal chemical reporters that enable direct in-g

26 provides a versatile general approach to map bioorthogonal-chemically engineered post-translational m

27 Bioorthogonal chemistries have been widely used to probe

28 Bioorthogonal chemistries have provided tremendous insig

29 openone ligation is also distinct from other bioorthogonal chemistries in that it makes use of readil

30 luorescent probes can make use of selective, bioorthogonal chemistries to report on specific analytes

31 a non-natural amino acid and two compatible bioorthogonal chemistries, we conjugated mannitol dehydr

32 aheterocycles, offering new opportunities in bioorthogonal chemistry and biological studies.

33 Our studies define a novel intersection of bioorthogonal chemistry and metabolite reactivity that m

34 tions of triazines have wide applications in bioorthogonal chemistry and natural product synthesis.

35 trazines (PMT) are a key enabler for in vivo bioorthogonal chemistry based on the very fast and catal

36 Bioorthogonal chemistry enables a specific moiety in a c

37 Bioorthogonal chemistry has become one of the main drivi

38 oteins and generate iodinated tetrazines for bioorthogonal chemistry has been developed.

39 ecent advances in genetic code expansion and bioorthogonal chemistry have enabled the site-specific l

40 luorescent probes designed for activation by bioorthogonal chemistry have enabled the visualization o

41 r strategy has been used in conjunction with bioorthogonal chemistry to image the external glycosylat

42 ting properties of hydroxamic acid groups by bioorthogonal chemistry using Pd-functionalized resins.

43 Combining bioorthogonal chemistry with the use of proteins enginee

44 These probes, in conjunction with bioorthogonal chemistry, enable selective in situ detect

45 Cyclopropenones are attractive motifs for bioorthogonal chemistry, owing to their small size and u

46 The feasibility of Affibody-based bioorthogonal chemistry-mediated pretargeting was demons

47 ling of RNA can be profiled and imaged using bioorthogonal chemistry.

48 , yet chemically tractable using copper-free bioorthogonal chemistry.

49 e trans-cyclooctene s-TCO are widely used in bioorthogonal chemistry.

50 that uses metabolic labelling combined with bioorthogonal click chemistry (that is, reactions perfor

51 We have developed a modification of bioorthogonal click chemistry to assay the palmitoylatio

52 ed metabolic oligosaccharide engineering and bioorthogonal click chemistry to label various commensal

53 ibodies, complementary oligonucleotides, and bioorthogonal click chemistry.

54 and Diels-Alder reaction, and other types of bioorthogonal click ligations have had a significant imp

55 he potential of boronic ester formation as a bioorthogonal click reaction between abiotic boronic aci

56 uorescence (NIRF) dye-labeled molecules by a bioorthogonal click reaction in cell cultures and in tum

57 Recently, metabolic glycoengineering with bioorthogonal click reactions has focused on improving t

58 Both components are cross-linked by multiple bioorthogonal click reactions in situ on the surface of

59 ceptor (EGFR) Fab and an anti-CD105 Fab, via bioorthogonal "click" ligation of trans-cyclooctene and

60 Fast, high-yielding, and selective bioorthogonal "click" reactions employing nontoxic reage

61 a wide range of biaryl sulfonyl fluorides as bioorthogonal "click" reagents.

62 7-octadecynoic acid (17-ODYA) can serve as a bioorthogonal, click chemistry probe for in situ labelin

63 rotecting group from a protein or drug under bioorthogonal conditions enables accurate spatiotemporal

64 Bioorthogonal conjugation chemistry has enabled the deve

65 ed from the rest of the protein pool through bioorthogonal conjugation of the azide side chain to pro

66 ironment is examined, and its potential as a bioorthogonal conjugation reaction is demonstrated.

67 in we report a fast (>10(3) M(-1) s(-1)) and bioorthogonal conjugation reaction that joins semicarbaz

68 oline derivatives were synthesized to permit bioorthogonal conjugation reactions, including azide-alk

69 etrazine moiety, which allowed site-specific bioorthogonal conjugation to an engineered membrane prot

70 bination of metabolically labeled glycans, a bioorthogonal copper(I)-catalyzed azide-alkyne cycloaddi

71 bsequently be conjugated to fluorophores via bioorthogonal copper-catalyzed click-chemistry.

72 The approach takes advantage of bioorthogonal coupling reactions to generate proteolipos

73 nd relied on copper-free click chemistry for bioorthogonal covalent cross-links throughout the film t

74 ectively captured protein occurs through the bioorthogonal CR group upon reaction with an appropriate

75 functionalized Pt(II)-based agents using the bioorthogonal Cu(I)-catalyzed azide-alkyne cycloaddition

76 Here we employ an optimized first-order bioorthogonal cyclization reaction to control the self-a

77 yl-cyclopropene groups that rapidly react by bioorthogonal cycloaddition in the presence of complemen

78 action can also be used in tandem with other bioorthogonal cycloaddition reactions.

79 , IEDDA reactions have recently found use in bioorthogonal decaging to activate proteins or drugs in

80 ogy for pretargeted PET imaging based on the bioorthogonal Diels-Alder click reaction between tetrazi

81 est brightness enhancements reported for any bioorthogonal fluorogenic dyes.

82 c oligosaccharide engineering to introduce a bioorthogonal functional group, the azide, into cellular

83 However, the slow reactivity of the bioorthogonal functional groups that can be encoded gene

84 ercially available amines, including several bioorthogonal functional groups.

85 re analogues of monosaccharides that contain bioorthogonal functionalities and enable the direct visu

86 robenzene (CDNB), and bear a wide variety of bioorthogonal functionalities such as alkynes, aldehydes

87 e of alternative substrates that incorporate bioorthogonal functionality including azides and alkynes

88 Here we report the in vitro bioorthogonal generation of 5-fluorouracil from a biolog

89 inhibitor conjugate contains a complementary bioorthogonal group.

90 The site-specific incorporation of bioorthogonal groups via genetic code expansion provides

91 al motifs, isotopic labels, and a variety of bioorthogonal handles are prepared, along with analogues

92 amino acids are used for the introduction of bioorthogonal handles into proteins.

93 y trap interactions in vivo and by employing bioorthogonal handles to enrich the protein targets dire

94 at Affibody-based pretargeting mediated by a bioorthogonal interaction between trans-cyclooctene (TCO

95 s functionalized tetrazines in vivo, via the bioorthogonal inverse electron demand Diels-Alder (IEDDA

96 Simultaneous systemic administration of the bioorthogonal IONPs in tumor-bearing mice demonstrated t

97 rface endogenous RNAs (e.g., microRNAs) with bioorthogonal l-DNA, suggesting applications in bioengin

98 l-fatty acid chemical reporters and improved bioorthogonal labeling conditions using the Cu(I)-cataly

99 Metabolic and bioorthogonal labeling methods have previously enabled t

100 strategy, which employs genetic encoding and bioorthogonal labeling of azF in live cells, should be u

101 interaction is utilized for highly efficient bioorthogonal labeling of cellular targets.

102 zed genetic code expansion and site-specific bioorthogonal labeling of rhodopsin with Alexa488 to ena

103 Genetic code expansion and bioorthogonal labeling provide for the first time a way

104 This work therefore extends the use of bioorthogonal labeling strategies to problems of clinica

105 hat tolerate SphK mutagenesis and subsequent bioorthogonal labeling.

106 evelopment in the context of chemoselective, bioorthogonal labeling.

107 idely deployed for alkyl-diversification and bioorthogonal labeling.

108 Here we introduce genetically directed bioorthogonal ligand tethering (BOLT) and demonstrate se

109 Here we report a novel bioorthogonal ligation between cyclopropenones and funct

110 chlorin to a cell penetrating peptide, using bioorthogonal ligation chemistry.

111 Metabolic glycan engineering (MGE) and bioorthogonal ligation have enabled study of biological

112 Bioorthogonal ligation methods with improved reaction ra

113 This bioorthogonal ligation of Tet-v2.0-protein reacts in cel

114 esis, as well as a simple protocol for their bioorthogonal ligation to a model saccharide using a Hui

115 Subsequent bioorthogonal ligation with alkyne-functionalized probes

116 -monophosphate (AMP) allows their subsequent bioorthogonal ligation with azide-fluorescent dyes or af

117 Fluorogenic bioorthogonal ligations offer a promising route towards

118 We have used a bioorthogonal metabolic labeling strategy to detect cell

119 ity, in conjunction with a more reproducible bioorthogonal method of chemical modification and facile

120 Although bioorthogonal methods that use engineered amino acids of

121 is resistant to TFA and thus can serve as a bioorthogonal modification of bioactive peptides.

122 The described bioorthogonal modification of CV with trans-cyclooctene

123 combination of primer extension, bypass, and bioorthogonal modification works also for double and tri

124 The bioorthogonal modifications protect the isolated PG agai

125 The acid-stable ketals 17-19 are bioorthogonal modifications which reconstitute the biolo

126 ng metabolic labeling and a gadolinium-based bioorthogonal MRI probe.

127 , we report the fabrication of protein-sized bioorthogonal nanozymes through the encapsulation of hyd

128 This technique, termed bioorthogonal non-canonical amino acid tagging (BONCAT),

129 Bioorthogonal non-canonical amino-acid tagging can be co

130 Bioorthogonal noncanonical amino acid tagging (BONCAT) c

131 Here, we adapt bioorthogonal noncanonical amino acid tagging (BONCAT) t

132 d a time-selective proteome-labeling method [bioorthogonal noncanonical amino acid tagging (BONCAT)]

133 Modifying the previously developed bioorthogonal noncanonical amino acid tagging method, we

134 sly within the cytosol and mediate efficient bioorthogonal organometallic chemistries (e.g., allylcar

135 , is becoming increasingly important because bioorthogonal organometallic chemistry involves palladiu

136 table masking group for implementing in vivo bioorthogonal organometallic chemistry.

137 A bioorthogonal organometallic reaction is a biocompatible

138 he development and biological screening of a bioorthogonal palladium-labile prodrug of the nucleoside

139 l and DHHC5-GT (gene-trapped) mice using the bioorthogonal palmitate analog 17-octadecynoic acid.

140 Using a bioorthogonal palmitate reporter to quantify palmitoylat

141 Finally bioorthogonal pantetheine analogues were shown to target

142 shed labeling strategies that originated the bioorthogonal paradigm, we have recently proposed the us

143 use of heterogeneous palladium chemistry and bioorthogonal Pd(0)-labile prodrugs to develop spatially

144 ability that maintain robust reactivity with bioorthogonal phosphines.

145 trate a synthetic strategy that exploits two bioorthogonal photochemistries to achieve reversible imm

146 zed as robust polymer fibers via interfacial bioorthogonal polymerization employing the rapid cycload

147 o test novel tetrazines for use with in vivo bioorthogonal pretargeting strategies.

148 e bacterial cell surface by an extracellular bioorthogonal probe, as judged by confocal fluorescence

149 these strained alkynes identify them as new bioorthogonal probes that should facilitate the discover

150 This new bioorthogonal process based on miniscule components is t

151 f identifying substrates of individual PMTs, bioorthogonal profiling of protein methylation (BPPM) ha

152 To address such a need, we envisioned a Bioorthogonal Profiling of Protein Methylation (BPPM) te

153 toclick chemistry and afforded rapid (<10 s) bioorthogonal protein labeling.

154 Here, we report that bioorthogonal proteomics of macrophages with an improved

155 A new bioorthogonal reactant pair, spiro[2.3]hex-1-ene (Sph) a

156 Here we review this bioorthogonal reaction and its promising applications fo

157 usly developed a novel approach based on the bioorthogonal reaction between a radiolabeled tetrazine

158 We focused on the bioorthogonal reaction between a trans-cyclooctene (TCO)

159 The high-fidelity bioorthogonal reaction between the symmetric hydrophilic

160 ze a pretargeting strategy that exploits the bioorthogonal reaction between transcyclooctene (TCO) an

161 The ligation exploits the bioorthogonal reaction of aldehydes and alkoxyamines to

162 This bioorthogonal reaction presents broad applicability and

163 udies establish Cu-free click chemistry as a bioorthogonal reaction that can be executed in the physi

164 Described is a bioorthogonal reaction that proceeds with unusually fast

165 This ideal bioorthogonal reaction will enable the monitoring of a l

166 l sites of chain termination in vivo through bioorthogonal reaction with fluorescent cyclooctyne prob

167 o called "copper-free click chemistry", is a bioorthogonal reaction with widespread applications in b

168 Here we explore an alternative bioorthogonal reaction, the 1,3-dipolar cycloaddition of

169 Our strategy exploits a known bioorthogonal reaction, the 1,3-dipolar cycloaddition of

170 ted product can be further functionalized by bioorthogonal reactions between the aldehyde residue and

171 Alder (IEDDA) reaction stands out from other bioorthogonal reactions by virtue of its unmatchable kin

172 Bioorthogonal reactions for labeling biomolecules in liv

173 tages of this approach over state-of-the-art bioorthogonal reactions for protein labelling in vitro a

174 The development of bioorthogonal reactions has classically focused on bond-

175 In recent years, a number of bioorthogonal reactions have been developed, exemplified

176 Although a number of bioorthogonal reactions have been documented in literatu

177 system enabled a detailed comparison of the bioorthogonal reactions in live cells and informed the s

178 For the latter, the impacts of bioorthogonal reactions on drug design (i.e., in situ co

179 n to the impact of click chemistry and other bioorthogonal reactions on the study of biological syste

180 nse interest in the development of selective bioorthogonal reactions or "click" chemistry that can pr

181 click chemistry, the details and benefits of bioorthogonal reactions that have been developed, and ex

182 e proline derivatives allowed three parallel bioorthogonal reactions to be conducted in one solution.

183 reporters in conjunction with catalyst-free bioorthogonal reactions will greatly streamline protein

184 Ideal bioorthogonal reactions with high reaction rates, high s

185 and can be used directly in cells for rapid bioorthogonal reactions with tetrazines.

186 Subsequent bioorthogonal reactions with the fluorophore-conjugated

187 Bioorthogonal reactions, especially the Cu(I)-catalysed

188 Bioorthogonal reactions, including the strain-promoted a

189 heterocycles in synthesis and are popular as bioorthogonal reactions.

190 ogical processes via the cycloaddition-based bioorthogonal reactions.

191 s of times faster than established encodable bioorthogonal reactions.

192 is 3-7 orders of magnitude faster than many bioorthogonal reactions.

193 alkenes have become among the most valuable bioorthogonal reactions.

194 t the sites that can be labeled, while many "bioorthogonal" reactions for which a component can be ge

195 on maleimide-toxin conjugates, which include bioorthogonal reactive groups, are also found to block c

196 es were introduced in the VHH to function as bioorthogonal reactive groups.

197 enetically encoded unnatural amino acid with bioorthogonal reactivity and the inhibitor conjugate con

198 Rapid bioorthogonal reactivity can be induced by controllable,

199 The rapid kinetics, bioorthogonal reactivity, and electrochemical control pr

200 ag protein technology for directly comparing bioorthogonal reagent reactivity, specificity, and stabi

201 ring a novel way to modulate the activity of bioorthogonal reagents in the most fragile and complex o

202 A new class of bioorthogonal reagents, 1,2,4-triazines, is described.

203 Two new bioorthogonal reagents, methylcyclopropene and 3,3,6,6-t

204 elationship to pinpoint the linker, dye, and bioorthogonal reporter of choice for carrier protein lab

205 ith single-molecule sensitivity, combining a bioorthogonal, selective chemical labeling method of 5-h

206 n mammalian cells, and demonstrate the rapid bioorthogonal site-specific labelling of a protein on th

207 Fluorescent bioorthogonal smart probes across the visible spectrum w

208 ga-azide or alkyne substituents suitable for bioorthogonal Staudinger and Huisgen [3 + 2] cycloadditi

209 rotein inactive until deprotection through a bioorthogonal Staudinger reduction delivers the active w

210 jugated to Tyr3-octreotate (Y3-TATE) through bioorthogonal, strain-promoted alkyne azide cycloadditio

211 aman scattering (SRS), and covers the use of bioorthogonal tags to enhance sample detection, and rece

212 azine ligations have proven to be a powerful bioorthogonal technique for the detection of many labele

213 Bioorthogonal template-driven tetrazine ligations could

214 n this study, we have harnessed the power of bioorthogonal tethering to SNAP and CLIP protein tags to

215 We demonstrate that bioorthogonal tetrazine ligations can be utilized to rap

216 Herein we report a novel bioorthogonal tetrazine uncaging reaction that harnesses

217 These features fill current voids in the bioorthogonal toolkit.

218 s integrate a coumarin fluorochrome with the bioorthogonal trans-cyclooctene(TCO)-tetrazine chemistry

219 However, many popular bioorthogonal transformations are incompatible with one

220 eir ability to perform simultaneous multiple bioorthogonal transformations will empower the applicati

221 Bioorthogonal uncaging strategies have recently emerged

222 A bioorthogonal uncaging strategy is presented, which is t