ライフサイエンスコーパス: biophysical

1 , IK,L in type I hair cells exhibited unique biophysical activation properties, which were faithfully

2 led in-depth examination of melittin-induced biophysical alterations of the cells.

3 Extensive biophysical analyses and pharmacokinetic (PK) studies in

4 Biophysical analyses in combination with cell-based assa

5 Biophysical analyses of TRPV1 constructs reveal that the

6 Finally, our functional and biophysical analyses reveal the molecular target of disu

7 d single-molecule spectroscopy together with biophysical analyses to demonstrate the parity-dependent

8 d comprehensive mutational, biochemical, and biophysical analyses to gain insights into the role of t

9 Combined with complementary biochemical and biophysical analyses, our structural data suggest that P

10 s of the enzyme bound to both substrates and biophysical analysis characterize the different binding

11 ework presented here is highly versatile for biophysical analysis of binding interactions.

12 Biophysical analysis of constructs refolded in vitro dem

13 Integrating biophysical analysis of stomatal and vascular conductivi

14 such interactions, we carried out a detailed biophysical analysis of zippering, occurring either spon

15 Biophysical analysis, using small angle X-ray scattering

16 tactin, and its fold, we conducted extensive biophysical analysis.

17 d and explore cellular networks of arbitrary biophysical and architectural complexity using the logic

18 luded homology modeling, MD simulations, and biophysical and biochemical characterizations, we invest

19 Biophysical and biochemical data on WRC mutants confirm

20 Taken together, these structural, biophysical and biochemical data suggest a model where t

21 rial that can mimic, manipulate, and measure biophysical and biochemical function within the plasma m

22 Here we present a biophysical and biochemical interrogation of the structu

23 these hypotheses in vivo We applied in vitro biophysical and biochemical methods to elucidate the mec

24 In this study, in vitro biophysical and biochemical methods were utilized to elu

25 A mechanistic biophysical and biochemical model of canopy carbon excha

26 ns and provided structural insight for their biophysical and biochemical properties and reactivity.

27 d NSC328784 behave similarly to ZMC1 in both biophysical and cell-based assays and are heretofore nam

28 Taken together, these biophysical and cellular studies define the structural b

29 mates of the eddy-driven vertical pumping of biophysical and chemical tracers.

30 Using biophysical and crystallographic approaches, we identify

31 We outline biophysical and evolutionary rationale for broad variati

32 Biophysical and functional studies demonstrate that SCF(

33 iction of EP offers a novel way to integrate biophysical and genomic studies of protein-DNA binding.

34 bust NaV1.3 currents, as determined by their biophysical and pharmacologic properties.

35 Biophysical and pharmacological experiments indicate a p

36 ntities of the key transporters and of their biophysical and regulatory properties, and have been mod

37 oscopy would facilitate deeper insights into biophysical and simulation studies of protein dynamics a

38 ling during fumarate reduction, for detailed biophysical and structural characterization.

39 Here, we demonstrate, using both biophysical and structural methods, that binding A11G RN

40 ask, the material interface should mimic the biophysical and the biochemical properties of neural tis

41 The extensive biophysical and topological diversity of membrane protei

42 Biophysical and ultrastructural analysis of AVNFH bone r

43 y researchers using NMR to study structural, biophysical, and biochemical properties of biological ma

44 Loss of trees has significant ecological, biophysical, and biogeochemical consequences.

45 elegans coupled with versatile biochemical, biophysical, and genetic tools, we provide an account of

46 Here, we apply biochemical, biophysical, and imaging techniques to elucidate the rol

47 on and for a variety of biotechnological and biophysical applications.

48 roscopy have had limited success; however, a biophysical approach based on diffusion of selected prob

49 ultiple binding partners, and also suggest a biophysical approach to exploit innate binding pathways

50 We utilize a biophysical approach to study the interactions between i

51 rium exchange mass spectrometry, a sensitive biophysical approach, to probe the dynamics of a membran

52 h reconstituted core1 (alpha2betabeta') by a biophysical approach.

53 Several biophysical approaches are available to study protein-pr

54 Computational as well as biophysical approaches based on nuclear magnetic resonan

55 erimental technologies will be combined with biophysical approaches to generate quantitative models o

56 Using immunoassays and biophysical approaches, we and others have recently repo

57 simple and complementary to well-established biophysical approaches.

58 ability to activate mast cells is based on a biophysical as well as a biochemical mechanism.

59 Herein, we review biophysical aspects of NPC architecture and function and

60 Biophysical assays including amyloid kinetics, dot blot,

61 Using a combination of cell-based and biophysical assays together with structural techniques,

62 single hit (12) validated by biochemical and biophysical assays, we describe our efforts to develop a

63 Using cryo-electron microscopy and biophysical assays, we have undertaken a mechanochemical

64 irectly with model membranes in a variety of biophysical assays.

65 cell-penetrating peptide TAT increases their biophysical association with cell surfaces over very sho

66 ts increased mobility on DNA damage, but the biophysical basis for this behavior remains unknown.

67 To reveal the biophysical basis of enhanced protein solubility and fun

68 ation of a disordered PPI by considering the biophysical binding mechanism of an IDP to a structured

69 e report cryo-electron microscopy (cryo-EM), biophysical, biochemical, and cell biological studies of

70 Herein we describe the biophysical, biochemical, and computational approaches t

71 refore may function as a clinical diagnostic biophysical biomarker.

72 Biophysical cardiac cell models were created to simulate

73 and rapid device to analyze disease-mediated biophysical cellular changes in the clinical setting in

74 ssing the EphA2 receptor, to investigate how biophysical cellular changes resulting from NCR manipula

75 s mitotic deadhesion and rounding, and these biophysical changes, which depend on RhoA activation and

76 ork exposes a strategy for understanding how biophysical characteristics contribute to function.

77 n channel current (Icat) in SW982 cells with biophysical characteristics of heteromeric TRPC4/C1 chan

78 etermine whether an ionic current having the biophysical characteristics of Kir2.1 is capable of resc

79 e method that uses polarised light to assess biophysical characteristics of tissues.

80 e focused on improving binding affinities or biophysical characteristics.

81 MCLA-128 is highly stable and has excellent biophysical characteristics.

82 Extensive biophysical characterization and modeling support the fo

83 Biophysical characterization and protein-level analysis

84 tive cycles of rational inhibitor design and biophysical characterization led to the discovery of the

85 Biophysical characterization of a subset of the binder d

86 Biophysical characterization of COPII vesicles fractiona

87 The biophysical characterization of their movement along the

88 hylogenetic reconstruction, resurrection and biophysical characterization of two interacting disorder

89 Biophysical characterization shows that the best inhibit

90 These applications include functional and biophysical characterization studies for ligand screenin

91 These structures and accompanying biophysical characterization suggest that MARV VP35 is a

92 Biochemical and biophysical characterizations aided by computational mod

93 Biophysical characterizations and a cell viability assay

94 learned from the use of the CGMD method, the biophysical-chemical findings on lipid-protein interplay

95 dels of LSO neurons with different levels of biophysical complexity, from predominantly functional on

96 nalysis of the FtsLB complex, performed with biophysical, computational, and in vivo methods, that es

97 pe and cropping systems, further affected by biophysical conditions.

98 monstrates a cost-effective tool to estimate biophysical connectivity remotely, supporting coastal ma

99 mechanism may be understood based on general biophysical considerations.

100 findings are used to develop a model for the biophysical constraints governing off-target DNA binding

101 evolution can be analyzed within an in vitro biophysical context.

102 escribe its role in neocortex in relation to biophysical correlates of memory function.

103 sparingly available patient-derived CTCs, a biophysical CTC phenotype more lysis-resistant than brea

104 stiffness, thus parsing the role of specific biophysical cues as sensitive epigenetic drivers.

105 y that near-complete sets of biochemical and biophysical data containing structural restraints are no

106 also ameliorates age-related behavioral and biophysical deficits.

107 Although biophysical details of inhibitory neurons are becoming k

108 ponse, and understanding the structure-based biophysical determinants for their binding specificity a

109 Analysis of the biophysical determinants of cell migration show that nuc

110 Synthetic biology was founded as a biophysical discipline that sought explanations for the

111 To probe this biophysical effect directly, rather than using minor-gro

112 Their biophysical effects and medically interesting activities

113 A biophysical equation (Augmented Growth Equation) was pre

114 de novo using protein structure modeling and biophysical evaluation of enzyme-substrate complexes.

115 This is consistent with the lack of biophysical evidence for a central pattern generator, an

116 provide, to our knowledge, the first direct biophysical evidence that Death Receptor 5 TM-dimers ope

117 isordered regions, we conducted a battery of biophysical experiments on the EGFR and HER3 tails.

118 ope reporters (19F, 13C) that can be used in biophysical experiments such as protein NMR.

119 ists; such observations await a satisfactory biophysical explanation.

120 This increase was driven by biophysical factors related to treatment effects on cyan

121 ng approaches, we showed that adding EP as a biophysical feature can improve the predictive power of

122 assemblages, which are in turn stabilized by biophysical feedback mechanisms acting through pore char

123 ons the methodology as a valuable option for biophysical fragment screening.

124 ion has since been gleaned from biochemical, biophysical, genetic, and molecular studies.

125 t disease distribution and abundance through biophysical impacts on the vector and on the virus.

126 ment activities for their biogeochemical and biophysical impacts, the level of process understanding

127 external stimuli by combining molecular and biophysical information.

128 tion of DNA microviscosity provides critical biophysical insights into nuclear crowding, nucleic acid

129 sing examples where sequence analysis offers biophysical insights.

130 ut functionalization, suggesting a different biophysical interaction between exosomes and MVs with SA

131 The method is widely applicable to the biophysical investigation of complexes of disordered pro

132 Biophysical investigation of membrane proteins generally

133 ansitions, provided models for more detailed biophysical investigations.

134 on article by Beaven et al. in this issue of Biophysical Journal.

135 he advanced quantitative data of nonclinical biophysical kinetics tools, making it adaptable to both

136 ke all organisms, are subject to fundamental biophysical laws.

137 an experimental system which represents the biophysical limit of Earth's biosphere.

138 echanotype, have demonstrated the utility of biophysical markers in clinical and research application

139 Using various biochemical and biophysical means we confirmed that this engineered Env

140 Consistent with the cellular data, biophysical measurements (small-angle X-ray scattering,

141 Biophysical measurements and proteomic analysis revealed

142 gle-vesicle optical (by TIRF microscopy) and biophysical measurements of ATP release indicate that G1

143 recombinant zinc finger domain by performing biophysical measurements of in vitro DNA-binding affinit

144 By combining NMR spectroscopy, biophysical measurements, statistical inference, and mol

145 Biophysical measurements, super-resolution, and electron

146 d revealed lateral pressure as an underlying biophysical mechanism dictating how curvature and compos

147 he excitatory interneuron thus serves as one biophysical mechanism for the introduction or modulation

148 primate visual area V4 showing that a single biophysical mechanism may underlie these diverse neural

149 While the biophysical mechanism of bacterial chemotaxis has been w

150 erstanding and the incorporation of internal biophysical mechanisms in future model developments.

151 used as model organisms to explore both the biophysical mechanisms of animal magnetoreception and th

152 Although considerable study has probed the biophysical mechanisms of how eukaryotic cells generate

153 To better understand biophysical mechanisms of mechanosensory processing, we

154 erful technique for revealing the underlying biophysical mechanisms of membrane bending at physiologi

155 The function and biophysical mechanisms of most such modulations remain u

156 a single-molecule toolbox to investigate the biophysical mechanisms of RNA folding and unfolding, its

157 known about its dynamics and the underlying biophysical mechanisms.

158 he heterochromatin state, and we developed a biophysical method (gradient-seq) to discriminate subtyp

159 Here, using a combination of analytical and biophysical methods in vitro, we tested whether EVs isol

160 Biochemical and biophysical methods were used to determine the expressio

161 Using biochemical and biophysical methods, here we show that anionic phospholi

162 We employed complementary biophysical methods, including atomic force microscopy,

163 proach, fluorescence spectroscopy, and other biophysical methods, including heterocomplex pulldown as

164 By coupling biophysical methods, super-resolution microscopy and phy

165 By using biochemical, structural, and biophysical methods, we present a structural model of th

166 ll angle X-ray scattering, and complementary biophysical methods.

167 on Ube2T through a fragment screening using biophysical methods.

168 to resolve the processes recorded by diverse biophysical methods.

169 torical limits of acceptability for multiple biophysical metrics of "developability." Amino acid sequ

170 We used a biophysical model based on mass transfer theory to show

171 Interestingly, our biophysical model for the swimming dynamics of B. burgdo

172 Here, we develop a biophysical model of a dopamine neuron incorporating ion

173 We have developed a biophysical model of kinetochore capture in small fissio

174 called BEESEM that builds on a comprehensive biophysical model of protein-DNA interactions, which is

175 Furthermore, we introduce a biophysical model of the zippering dynamics, and we quan

176 In one biophysical model, the concentration of mucin on the air

177 tionally efficient approximation of the full biophysical model.

178 Here we investigate by biophysical modeling the origin of the focal LFP signatu

179 By combining our measurements with biophysical modeling, we determined that the ribosomal f

180 ime) results were coupled to straightforward biophysical models of protein aggregation.

181 More recently, biophysical models of protein evolution, which combine e

182 Here the authors construct biophysical models of pyramidal neurons that reproduce o

183 s, but new high-throughput methods allow for biophysical models with increased accuracy that should b

184 This report describes the biophysical nature of sugar-channel interactions and the

185 e through its binding antigens, altering the biophysical nature of the target cell and thus reducing

186 We show that biophysical niche factors act as a switch to toggle hPSC

187 properties of neural response thought to be biophysical nuisances can confer coding advantages to ne

188 Realistic neuronal simulations of the biophysical observations recapitulated the elevated acti

189 Here, we integrate satellite-derived biophysical observations, particle dispersion model simu

190 f researchers who study either single-neuron biophysical, or neural circuit, mechanisms that can gene

191 forward-modeling scheme, we investigated the biophysical origin of this stLFP signature with simultan

192 d sound lateralization to their cellular and biophysical origins.

193 he cell wall and the fine-tuning of cellular biophysical parameters in the mutants compared to the wi

194 The engineering approach based on biophysical parameters resulted in a peptide that is a h

195 tches at PAM-distal bases modulate different biophysical parameters that determine association and di

196 ally significant differences in quantifiable biophysical parameters, such as matter density and cell

197 Two biophysical parameters, the diffusion coefficient and th

198 to its suggested functional role in numerous biophysical phenomena and its importance as a general me

199 s combines transcriptome-wide throughput and biophysical precision.

200 lity can be rationalized from structural and biophysical principles.

201 brought to light an interesting set of novel biophysical principles.

202 ful modeling provides additional support for biophysical principles.

203 ey the methodologies for labeling GPCRs with biophysical probes, particularly fluorescent dyes, and h

204 lectrical transduction in the inner ear is a biophysical process underlying the senses of hearing and

205 ts, allowing a wide range of biochemical and biophysical processes and interactions to be studied at

206 To better understand the governing biophysical processes and their relative importance, it

207 of apoptosis, a better understanding of the biophysical processes involved may lead to new avenues f

208 is in southern Amazonia, changes in internal biophysical processes, light-harvesting adaptations (e.g

209 eting and fluorescence, characterizing their biophysical properties and imaging them using ultrasound

210 utward rectifying K(+) current, IK,L , whose biophysical properties and molecular identity are still

211 LCR mutations, which systematically tune its biophysical properties and Pab1 phase separation in vitr

212 very system presented here possesses notable biophysical properties and potency, and provide a platfo

213 2+) spark/STOC production depends upon their biophysical properties and the resting membrane potentia

214 sed these nanocomplexes still retained their biophysical properties and transfection efficiencies.

215 on approach based on utilization of inherent biophysical properties as 'markers' for adulteration.

216 We propose that emergent biophysical properties associated with phase-separated s

217 s formed from wild-type TDP-43 show distinct biophysical properties depending on axonal location, sug

218 ic terminal in the auditory brainstem, whose biophysical properties have been well studied in slices.

219 Furthermore, the biophysical properties imparted to plasma membranes are

220 tion in a site-specific manner affects tau's biophysical properties in vitro Our findings indicate th

221 culations demonstrate that the structure and biophysical properties of a correctly assembled c-ring a

222 In this Communication we describe the biophysical properties of a hydride-bound state (Hhyd) o

223 n its unusual structure, we investigated the biophysical properties of At2g44920 as a representative

224 r, death produces significant changes in the biophysical properties of brain tissues, making an ex vi

225 This study directly compares biophysical properties of electric fields arising from t

226 ctly test five major models by measuring the biophysical properties of fluorescently tagged BMP2b and

227 ercellular K(+) concentrations, altering the biophysical properties of IK,L .

228 e first complete description of the 'native' biophysical properties of IK,L in adult mouse vestibular

229 We found that the biophysical properties of IK,L were affected by an unsta

230 elastic back-scattered light, changes in the biophysical properties of lipid bilayers were revealed.

231 onin (5-HT), for its ability to modulate the biophysical properties of MCs across the MOB and AOB, us

232 show that QD labeling does not affect major biophysical properties of nanoparticles or their interac

233 methodology will also be used to enhance the biophysical properties of other recalcitrant vaccine can

234 have profound effects on the biological and biophysical properties of proteins associated with misfo

235 al dynamics provides important insights into biophysical properties of the active cytoplasmic medium.

236 izes and sorts a client protein based on two biophysical properties of the client's misfolded state (

237 cells and living animals, allowing to probe biophysical properties of the cytosol.

238 cytometry and flicker spectroscopy to define biophysical properties of the erythrocyte, we show that

239 The overall biophysical properties of the pores were similar for all

240 thermal calorimetry, to characterize various biophysical properties of the skeletal DHPR beta subunit

241 n membranes that mimic lipid composition and biophysical properties of the viral envelope.

242 amyotrophic lateral sclerosis (ALS), but the biophysical properties of these proteins have not yet be

243 ton microscopy, to compare photochemical and biophysical properties of various iRFPs.

244 cted using whole cell patch-clamp to explore biophysical properties of wild-type and mutant KV 3.1 ch

245 We show here that specific biophysical properties regulate the in vivo efficacy of

246 p40 and p19 specific reagents, and maintains biophysical properties similar to wild-type IL-23 (monom

247 uid droplets and therefore has the necessary biophysical properties to undergo liquid-liquid phase se

248 ight the diversity, including differences in biophysical properties, that drive distinct biological f

249 model systems to compare and contrast their biophysical properties.

250 ticulum (ER) due to its uniquely challenging biophysical properties.

251 king, and modulate receptor mobility and its biophysical properties.

252 l voting into IRaPPA, a pipeline integrating biophysical properties.

253 r native BslA correlates with their in vitro biophysical properties.

254 li in very large amounts, and show excellent biophysical properties.

255 nical challenges such as low potency or poor biophysical properties.

256 duction of protein variants with compromised biophysical properties.

257 ity of a monoclonal antibody is an important biophysical property relevant for its developability int

258 s in AR and GR genomic binding and provide a biophysical rationale for how promiscuous binding by GR

259 These biophysical signatures have the potential to be used as

260 acellular dynamics and thereby create unique biophysical signatures, which are altered in many diseas

261 highly coarse-grained model that retains key biophysical SNARE properties such as the zippering energ

262 ep forward, demonstrating the development of biophysical soil accounts that can be used in wider soci

263 are tightly linked to shear stress, the key biophysical stimulus from blood flow.

264 Complementary biophysical, structure-function, and kinetic analysis de

265 egulate all aspects of human physiology, and biophysical studies have deepened our understanding of G

266 Earlier biochemical and biophysical studies have shown that GPCRs exist temporal

267 Recent biochemical and biophysical studies indicate that MAGEs assemble with E3

268 tool for a broad spectrum of biochemical and biophysical studies of membrane proteins and are commonl

269 emonstrate how powerful this organism is for biophysical studies of self-organization and pattern for

270 Biophysical studies on model membranes revealed SM and C

271 Solution-phase biophysical studies reveal that the stabilized trimers a

272 DFT methods, have even more capabilities for biophysical studies, as their altered pKa values, relati

273 To enable more detailed biochemical/biophysical studies, Myo2 was expressed in the baculovir

274 gradation but, together with biochemical and biophysical studies, reveals distinct roles for residues

275 in RNA structure determination, probing, and biophysical studies, the past decade has seen remarkable

276 ed in tissue engineering and as matrices for biophysical studies.

277 icine and developmental biology but also for biophysical studies.

278 A combined biochemical, computational, and biophysical study now reveals that YopO-mediated phospho

279 Here, we report a biochemical and biophysical study of carbapenem hydrolysis by the B1 enz

280 e that our work provides a framework for how biophysical systems can use non-equilibrium driving to a

281 Using biophysical techniques and an expression and purificatio

282 Analysis of the PGR using biophysical techniques demonstrated the region is a high

283 Orthogonal biophysical techniques have been used to prove the "junc

284 ng single-molecule fluorescence and ensemble biophysical techniques, and a wide range of pH and tempe

285 scs and their subsequent analysis by several biophysical techniques, can serve as a versatile screeni

286 ymnosperms are limited, our state-of-the-art biophysical techniques, coupled with expression studies,

287 are studied with a number of structural and biophysical techniques, many requiring protein overexpre

288 ation of electrophysiological, cellular, and biophysical techniques, we show that the flexible, N-ter

289 bination of approaches including a number of biophysical techniques, we show that there are two disti

290 omplex with RFs, using an integrated host of biophysical techniques.

291 are not accessible to other high-resolution biophysical techniques.

292 ltiple distinct oscillations, we developed a biophysical thalamic model to test the hypothesis that g

293 in the PAR network have begun to merge with biophysical, theoretical and computational efforts to un

294 Biophysical titrations reveal that the 5'-AUAGC-3' bulge

295 r nucleoside probe represents a new class of biophysical tool that would complement available tools f

296 Using a diverse range of biochemical and biophysical tools involving circular dichroism, fluoresc

297 in stem cell culture methods, materials and biophysical tools that assess function have improved our

298 Here, we combine genetic approaches with biophysical tools to explore relationships between kinas

299 etry (HXMS) and a battery of biochemical and biophysical tools to investigate how post-translational

300 Together, these models provide a biophysical understanding of how stem cell scaling is ma