ライフサイエンスコーパス: biotinylation

1 says, gel filtration and proximity-dependent biotinylation.

2 carboxylase and catalyzes posttranslational biotinylation.

3 ecreased rate of endocytosis, as assessed by biotinylation.

4 yme with substrate limits post-translational biotinylation.

5 cell patch clamp recordings and cell surface biotinylation.

6 localized TRPC3, as determined using surface biotinylation.

7 ere further confirmed by basolateral surface biotinylation.

8 a67 fragment and BSA, and is subject to self-biotinylation.

9 or control, as determined by surface protein biotinylation.

10 al membrane CFTR as assessed by cell surface biotinylation.

11 py, resonance energy transfer, and proximity biotinylation.

12 e plasma membrane was tested by cell-surface biotinylation.

13 in WT but not in m1m2 cells, as measured by biotinylation.

14 cB in a complex that is a poor substrate for biotinylation.

15 e plasma membrane was tested by cell-surface biotinylation.

16 he plasma membrane, as shown by cell-surface biotinylation.

17 nly wild-type iNOS had increased levels of S-biotinylation.

18 as was accessibility of the Cys residues to biotinylation.

19 the presence of MA made Env inaccessible to biotinylation.

20 Surface NHE3 was determined by cell surface biotinylation.

21 for stabilizing spirolactones for subsequent biotinylation.

22 coupled with flow cytometry and cell surface biotinylation.

23 cence-assisted cell sorting and cell surface biotinylation.

24 ssion of UT-A1, as evidenced by cell surface biotinylation.

25 by immunofluorescence staining and membrane biotinylation, ACE2 protein was more abundantly expresse

26 Cell surface biotinylation after mechanical injury indicates that pro

27 ne, as SpoVAEa was accessible to an external biotinylation agent in spores and SpoVAEa disappeared in

28 Site-specific biotinylation allows us to specifically attach the myosi

29 es of exposure to PregS, as shown by surface biotinylation and affinity purification.

30 Cell membrane proteins were enriched by biotinylation and avidin precipitation and analyzed by t

31 PM-coated electrode through further surface biotinylation and bridging avidin or NeutrAvidin.

32 es of pulse-chase labelling, domain-specific biotinylation and cell fractionation.

33 We present a workflow for biotinylation and characterization of recombinant antibo

34 Furthermore, the biotinylation and co-immunoprecipitation combined assays

35 ing, immunogold electron microscopy, surface biotinylation and computational modeling, we demonstrate

36 sent on the cell surface, demonstrated using biotinylation and confocal imaging, as well as in the cy

37 ated conclusion is supported by cell surface biotinylation and confocal microscopy of endogenous hCTR

38 ve mutant S138C hRFCs, combined with surface biotinylation and confocal microscopy, a dominant-negati

39 E3 distribution was assessed by cell-surface biotinylation and confocal microscopy.

40 ) were immobilized in the plasma membrane by biotinylation and cross-linking with avidin.

41 Using surface biotinylation and dual immunofluorescence staining in MY

42 and confocal microscopy coupled with surface biotinylation and electrophysiology suggest that changes

43 Surface biotinylation and endoglycosidase digestion experiments

44 Cell surface biotinylation and endoglycosidase H analysis revealed a

45 Cell surface biotinylation and immunoblotting with epitope-specific a

46 Cell surface biotinylation and immunofluorescence confocal microscopy

47 Cell surface biotinylation and immunofluorescence experiments in cell

48 Arterial biotinylation and immunofluorescence indicated that TRPP

49 In HTR cells, combined biotinylation and immunoprecipitation studies revealed p

50 In the present work we employ cell surface biotinylation and isotopic copper uptake studies of over

51 els of APP, as determined by surface protein biotinylation and live cell staining.

52 of ApoEr2, as determined by surface protein biotinylation and live cell surface staining.

53 resent here an in vitro method that combines biotinylation and mass spectrometry (MS) to identify sub

54 Studies applying in vivo biotinylation and mathematical modeling showed that newb

55 Using biotinylation and membrane fractionation assays, prolong

56 anol, and surface receptors were isolated by biotinylation and P2 fractionation, whereas functional a

57 Cell surface biotinylation and selective inactivation of surface EGFR

58 Surface biotinylation and streptavidin pull-down of cells expres

59 Site-specific biotinylation and streptavidin staining take only a few

60 This method is based on biotinylation and streptavidin-fluorochrome labeling of

61 ealthy and CF donors was assessed by surface biotinylation and subsequent Western blot analysis.

62 Using plasma membrane biotinylation and super-resolution microscopy, we demons

63 cessibility technique combining cell-surface biotinylation and tandem-affinity purification to study

64 al membrane protein expression, we performed biotinylation and total internal reflection fluorescence

65 Combined approaches of cell surface biotinylation and transport analysis were employed for s

66 Cell surface localization was analyzed by biotinylation and trypsin cleavage of extracellular cadh

67 Using biotinylation and two-electrode voltage-clamp on Xenopus

68 units and their endocytosis were measured by biotinylation and Western blots.

69 chemistry, single-particle tracking, surface biotinylation, and calcium imaging.

70 ical digestion, Cu(II)-mediated spirolactone biotinylation, and enrichment by avidin affinity chromat

71 e-cell patch clamp analysis, immunostaining, biotinylation, and immunoprecipitation methods to invest

72 e, a putative role for the enzyme in histone biotinylation, and its participation as a nuclear factor

73 y DOR immunohistochemistry, cell-surface DOR biotinylation, and live imaging of neurons transfected w

74 graphy, co-immunoprecipitation, cell surface biotinylation, and metabolic labeling.

75 were confirmed in human DAT-N2A cells using biotinylation, and similar effects were detected in rat

76 patch clamp electrophysiology, cell surface biotinylation, and total internal reflection fluorescenc

77 orientation using the chemical labeling and biotinylation approach, the "Cys-null" mutant of SNAT4 w

78 ing experiments with domain-specific surface biotinylation as well as immunocytochemical analysis of

79 ed in the plasma membrane using cell surface biotinylation as well as in intracellular compartments,

80 trate the time scales required for efficient biotinylation as well as the hazards of overbiotinylatio

81 This was confirmed using a surface protein biotinylation assay and Brefeldin A disruption of the ro

82 A cell surface biotinylation assay and live cell confocal imaging studi

83 Finally, the in-cell biotinylation assay did not provide any evidence for the

84 Biotinylation assay further showed that POSH decreased s

85 ng, and surface expression was analyzed by a biotinylation assay in cRNA-injected Xenopus laevis oocy

86 Surface biotinylation assay showed an increased level of SNAT3 o

87 o subapical intracellular compartment, and a biotinylation assay showed approximately 83% lower surfa

88 A biotinylation assay suggested that insulin and IGF1 inhi

89 rat ASIC3 (nmrASIC3) and used a cell-surface biotinylation assay to demonstrate that it traffics to t

90 and Fmr1 KO mice and immunocytochemistry and biotinylation assay to study related changes of 2-amino-

91 Using a reversible biotinylation assay, we quantified internalized hCTR1, w

92 hypothesis we developed a compartmentalized biotinylation assay, which we used to show that the C-te

93 hippocampal membranes was determined using a biotinylation assay.

94 MDA on TRPC6 was observed using cell surface biotinylation assays and also with whole-cell recordings

95 ar compartments, as assessed by cell-surface biotinylation assays and confocal microscopy.

96 Membrane biotinylation assays and immunostaining showed that endo

97 In vivo biotinylation assays confirmed that nephrin expression d

98 ATPase and biotinylation assays in mpkCCD(c14) cells demonstrated t

99 Single-channel analysis and biotinylation assays indicate that MPS-1 reduces the mac

100 Biotinylation assays revealed a significant increase in

101 TIRFM and biotinylation assays show robust receptor- and store-dep

102 Biotinylation assays showed reduced cell surface abundan

103 ernal Reflection Fluorescence microscopy and biotinylation assays showed that CASK silencing increase

104 studies using HC-3 binding and cell surface biotinylation assays showed that the PS-1 mutation inhib

105 og JHC1-64 and by reversible and pulse-chase biotinylation assays showing evidence for lysosomal degr

106 used both immunofluorescence microscopy and biotinylation assays to assess the trafficking of ClC-3.

107 Results of surface biotinylation assays using COS-7 cells suggest that Kv4.

108 Proximity biotinylation assays with LMP1 induced biotinylation of

109 metabolic labeling coupled with cell surface biotinylation assays, fenofibrate did not inhibit SR-BI

110 tested in metabolic pulse-chase and surface biotinylation assays, respectively.

111 ns on the cell surface were shown by surface biotinylation assays.

112 and total Slo1 as indicated by cell-surface biotinylation assays.

113 RPC3 membrane insertion, as shown by surface biotinylation assays.

114 specificity compared with the commonly used biotinylation-based assays.

115 Biotinylation-based recycling and degradation studies in

116 Here, we use proximity-dependent biotinylation (BioID) to map the centrosome-cilium inter

117 coimmunoprecipitation (co-IP) and proximity biotinylation (BioID), to identify viral and cellular pr

118 e solvent in free Gag and are protected from biotinylation by direct protein-ligand or protein-protei

119 After biotinylation by exposure to extracellular MTSEA-biotin

120 ety of recombinant proteins and demonstrated biotinylation by yBL at the N-terminus, C-terminus, and

121 was at least 3-fold better using C-terminal biotinylation compared with tagging at the N terminus us

122 P) sensor chips, we investigated a number of biotinylation conditions for target ligands.

123 Surface biotinylation confirmed the locus of RARalpha expression

124 Using cell surface protein biotinylation coupled with immunoblotting and immunofluo

125 Using a novel application of surface biotinylation, data indicated that >95% of Ca(V)1.2 alph

126 Pulse labeling with [(35)S]methionine and biotinylation demonstrated that about 25% of newly synth

127 Cell surface biotinylation demonstrated that KCa2.3 was rapidly endoc

128 Membrane biotinylation demonstrated that nonfunctional mBest3 pro

129 Surface biotinylation demonstrated the loss of ENaC from the api

130 The position of Fab biotinylation dictates the geometry of multimer assembly

131 Biotinylation experiments also revealed an increase in s

132 Cell-surface protein biotinylation experiments and immunofluorescence studies

133 Biotinylation experiments and single-channel analysis re

134 thin endosomal compartments, whereas surface biotinylation experiments confirmed that phosphorylated

135 Consistent with this possibility, biotinylation experiments demonstrated that soluble Dsg3

136 Immunofluorescence studies and surface biotinylation experiments demonstrated that the mutant p

137 tibody uptake experiments as well as surface biotinylation experiments demonstrated that the region b

138 Biotinylation experiments demonstrated that this result

139 Biotinylation experiments followed by immunostaining by

140 Biotinylation experiments in COS-7 cells show that altho

141 Indeed, pulse-chase biotinylation experiments in IECs lacking AnxA2 demonstr

142 supported by confocal imaging and reversible biotinylation experiments in transfected differentiated

143 Pulse-chase labeling and cell surface biotinylation experiments indicated that in the absence

144 Cell surface biotinylation experiments on KChIP4a indicate that the N

145 Biotinylation experiments revealed that the rapid increa

146 Western blotting and surface biotinylation experiments show that loss of myoferlin re

147 Cell surface biotinylation experiments showed approximately 45% reduc

148 Surface biotinylation experiments suggest that the non-wild-type

149 A series of cell-surface biotinylation experiments suggests that anterograde traf

150 Combining with oocyte surface biotinylation experiments, we demonstrated that RACK1 in

151 in mouse cortical cultures as determined by biotinylation experiments.

152 icing assays, deletion mapping, cell-surface biotinylation, expression studies and molecular modellin

153 Chemical biotinylation followed by enrichment and mass spectromet

154 Maleimide-PEO2-biotin precipitated (surface biotinylation followed by Western blotting) and reduced

155 n-protein interaction detection by proximity biotinylation has the advantages of low background, high

156 Both real-time imaging and surface biotinylation have demonstrated internalization of alpha

157 Metabolic labeling, cell surface biotinylation, immobilized lectins, and confocal immunof

158 Importantly, cell surface biotinylation, immunofluorescence and down-regulation ex

159 Biotinylation, immunofluorescence resonance energy trans

160 Immunoprecipitation, cell surface biotinylation, immunofluorescence, and functional assays

161 Using surface biotinylation, immunohistochemistry, electron microscopy

162 rotein partners, we utilized BioID proximity biotinylation in combination with stable isotopic labeli

163 munolocalization in oocytes and cell surface biotinylation in HEK cells indicated that the WNK-mediat

164 Cell-surface biotinylation in hepatocytes confirmed that ABCC6 is int

165 e and function of Arc1p can be modulated via biotinylation in response to temperature changes.

166 residues of beta protein are protected from biotinylation in the DNA complex, whereas none of the ly

167 approach based on cell type-specific in vivo biotinylation in zebrafish to analyse the initial respon

168 indirect immunofluorescence and cell surface biotinylation indicated that the surface pool was much s

169 ther, these results indicate that C-terminal biotinylation is a promising tagging strategy for develo

170 rent molecular weight (MW), 66 kDa], surface biotinylation labeled an ERalpha-immunoreactive protein

171 m 30 to 37 degrees C drastically reduced its biotinylation level.

172 escribe an NBS specific UV photocrosslinking biotinylation method (UV-NBS(Biotin)) for the oriented i

173 ndent uptake of (14)C-TC, and a cell surface biotinylation method was used to determine Ntcp and Mrp2

174 e as well as the less commonly used carboxyl biotinylation methods.

175 ts but are not biotinylated due to a mutated biotinylation motif.

176 gulators of ACCase after initial loss of the biotinylation motif.

177 To address this issue, we have used MTSEA-biotinylation (N-Biotinoylaminoethyl methanethiosulfonat

178 Likewise, biotinylation occurred after treatment with beta-mercapt

179 ated; however, in the absence of either one, biotinylation occurred.

180 Apical biotinylation of (MPK)CCD(14) cells confirmed the loss o

181 interactions based on interaction-dependent biotinylation of a peptide by E. coli biotin ligase, to

182 id metabolism through the post-translational biotinylation of acyl coenzyme A carboxylases.

183 Here we used a proteomic screen with in vivo biotinylation of AID to identify the splicing regulator

184 s interact, BirA will catalyze site-specific biotinylation of AP, which can be detected by streptavid

185 Nevertheless, biotinylation of Arc1p was temperature dependent; raisin

186 Biotinylation of cell surface proteins revealed decrease

187 esis under native and denaturing conditions, biotinylation of cell surface proteins, and immunostaini

188 n internalization at the BTB was assessed by biotinylation of cell surface proteins, to be followed b

189 yonic stem (mES) cells, we have used in vivo biotinylation of critical transcription factors for affi

190 e establishment of an assay that is based on biotinylation of effectors in the host cytoplasm as hall

191 apid and simple method that combines in vivo biotinylation of engineered host-specific bacteriophage

192 Biotinylation of free Ku revealed several reactive lysin

193 Biotinylation of histones plays crucial roles in the rep

194 Moreover, biotinylation of intestinal luminal proteins from mice f

195 Metabolic biotinylation of intracellular and secreted proteins as

196 inding regions of beta protein further using biotinylation of lysine residues and mass spectrometry.

197 Biotinylation of MV4-11 cell surface proteins followed b

198 on activation, the APEX enzyme catalyzes the biotinylation of neighboring endogenous proteins that ca

199 ion of oxidized proteins, which involves (1) biotinylation of oxidized proteins with biotin hydrazide

200 The biotinylation of PCFT was further confirmed by blocking

201 onclusions are based on studies in which (i) biotinylation of peritoneal macrophages showed that endo

202 ion of the biotin-swap technique, comprising biotinylation of protein SNO-Cys residues, trypsinolysis

203 Here, we show that metabolic biotinylation of proteins fused to the bacterial biotin

204 BioID features proximity-dependent biotinylation of proteins that are near-neighbors of the

205 Using cell-surface protein biotinylation of rat cerebellar slices, we found secreti

206 Biotinylation of surface proteins demonstrates that cell

207 bacterium's DC surface proteome by selective biotinylation of surface proteins, NeutrAvidin affinity

208 ophysiology, immunofluorescent staining, and biotinylation of surface receptors.

209 In this study, the selective biotinylation of surface-exposed proteins, streptavidin

210 By contrast, biotinylation of the acetyl-CoA carboxylase 1 and 2 (ACC

211 imity biotinylation assays with LMP1 induced biotinylation of the actin-associated proteins, which we

212 The results demonstrate that biotinylation of the BCCP fragments of the mitochondrial

213 -AMP from biotin and ATP, and the succeeding biotinylation of the biotin carboxyl carrier protein.

214 Simple biotinylation of the DNA template allows for labeling of

215 inylation of TMD2 mutants G93C and F94C, and biotinylation of these residues inhibited methotrexate t

216 Pemetrexed pretreatment inhibited biotinylation of TMD2 mutants G93C and F94C, and biotiny

217 OMP-1B was undetectable either by surface biotinylation or by Western blotting of the whole bacter

218 a membrane compared to a sulfo-NHS-activated biotinylation or two-step SEEL.

219 the predicted C-terminal SAP domain of Ku70, biotinylation patterns were observed which suggest a str

220 ssociation rates with HCS in order to ensure biotinylation prior to mitochondrial import.

221 omponents, such as Tris and glycerol, on the biotinylation process.

222 ) as the ligand of GPR56 through an in vitro biotinylation/proteomics approach.

223 We also demonstrate that the biotinylation range of BioID2 can be considerably modula

224 e two recombinant BirA proteins catalyze the biotinylation reaction of the acceptor biotin carboxyl c

225 abeled with an impermeant, cysteine-specific biotinylation reagent (MTSEA-biotin) with or without per

226 Access to a membrane-impermeant biotinylation reagent as well as protease sensitivity wa

227 gh these levels were not increased by higher biotinylation reagent concentrations or longer reaction

228 proteins, a cell-impermeant, thiol-reactive biotinylation reagent was used to label and subsequently

229 could be labeled with a membrane-impermeant biotinylation reagent, indicating surface exposure.

230 By using bulky biotinylation reagents on intact cells, we showed that t

231 hly efficient, identifying specific sites of biotinylation remains challenging.

232 Cell surface biotinylation results indicated that Orai1 channels in t

233 We used the in vivo biotinylation retro-translocation assay in mammalian cel

234 sistent with a reduction in current density, biotinylation revealed a significant reduction in surfac

235 Surface biotinylation revealed rapid loss of PAM-1/H3A, with no

236 Biotinylation reveals a 32-kDa, covalently cross-linked

237 Cell surface biotinylation reveals that plasma membrane GLUT1 levels

238 Cell surface biotinylation, sensitivity to glycosidases, and fluoresc

239 Confocal microscopy and surface-selective biotinylation showed that 80% of the surface amount of t

240 Surface biotinylation showed that dobutamine did not affect plas

241 A recombinant form of CPS containing a biotinylation site from an Escherichia coli protein is a

242 ffecting chromatin structure; at least seven biotinylation sites have been identified in human histon

243 crease of more than 30-fold in the number of biotinylation sites identified compared to streptavidin-

244 ent and mass spectrometry yielded over 1,600 biotinylation sites on hundreds of proteins, an increase

245 Thus, we used a novel biotinylation strategy to verify protein localization, a

246 Using a biotinylation strategy, it was found that surface expres

247 Plasma membrane biotinylation studies and confocal microscopic examinati

248 Importantly, surface biotinylation studies demonstrate that the membrane dist

249 Next, we performed surface biotinylation studies in acutely dissociated hippocampal

250 However, surface biotinylation studies in HIT-T15 cells indicate that Rem

251 l current density, immunohistochemistry, and biotinylation studies in isolated hearts and cardiomyocy

252 Ex vivo biotinylation studies in mouse striatal slices demonstra

253 Fluorescence flow cytometry and biotinylation studies revealed a rapid increase in DAT c

254 ax) of the transporter; whereas cell surface biotinylation studies revealed no alteration in the cell

255 Rather, cell surface biotinylation studies showed an increased number of CaV2

256 Biotinylation studies showed reduced SERT proteins in th

257 Cell surface biotinylation studies showed that both GLYX-13 and ketam

258 Using cell surface biotinylation studies, we detected endogenous sgk1 at th

259 neurons using the pHluorin-GluR1 imaging and biotinylation studies, we observed that prolonged morphi

260 BioTag, a 23-amino-acid peptide serving as a biotinylation substrate for BirA, in vivo in worms.

261 otin acceptor substrates and the kinetics of biotinylation suggests that mitochondrial carboxylase se

262 ee procedures will be described: (i) in vivo biotinylation system setup in mES cells; (ii) affinity p

263 oblem, we report here the use of a sensitive biotinylation system to probe the dislocation of major h

264 gamma containing a C-terminal biotinylation tag (gamma-C(tag)) was provided in trans a

265 By using an in vivo biotinylation tagging approach followed by liquid chroma

266 Using biotinylation tagging technology and high-throughput seq

267 proach using hexahistidine and BirA-specific biotinylation tags for isolating translocated effector c

268 protein denaturation using polyhistidine and biotinylation tags.

269 on of CD4 and BST-2/Tetherin using our novel biotinylation technique in living cells to determine ER-

270 channel in combination with fluorescence and biotinylation techniques in both human embryonic kidney

271 a2.3 using a combination of fluorescence and biotinylation techniques.

272 We used surface biotinylation to demonstrate that ERalpha has an extrace

273 transport characterization and cell-surface biotinylation to examine the residues involved in inhibi

274 In the present study, we used surface biotinylation to identify and study the estradiol regula

275 nd combined this technique with cell surface biotinylation to identify surface-exposed proteins of a

276 We have used cell surface biotinylation to label gap junction-unassembled plasma m

277 In this study, we used selective biotinylation to label proteins localized to the surface

278 cipitation and live cell proximity-dependent biotinylation to monitor interactions between Glrx3, Bol

279 We used cell surface biotinylation to monitor NPC1L1-GFP endocytosis and show

280 method along with surface and site-specific biotinylation to probe TM6 for aqueous accessibility and

281 imaging, co-immunoprecipitation, and surface biotinylation to study the functional consequences of a

282 Selectivity in HCS-catalyzed biotinylation to the carboxylases was investigated in si

283 We used proximity biotinylation to uncover new constituents of the inner n

284 Our results highlight the power of proximity biotinylation to yield insights into the molecular compo

285 Third, by cell surface biotinylation, trafficking of NHE3 was examined in short

286 ed GLIB (glucosylation, periodate oxidation, biotinylation) uses a combination of enzymatic and chemi

287 for photolabeling, and an alkyne moiety for biotinylation via "click chemistry".

288 S-Biotinylation was mapped to C104 and C109 on wild-type i

289 ferential detergent fractionation or surface biotinylation was used directly without immunoprecipitat

290 ular N-terminal GluA1 antibody or by surface biotinylation, was impaired following knockdown of CaMKK

291 unofluorescence, EM localization and surface biotinylation we show that STIM1 migrates from endoplasm

292 Using peroxidase-mediated proximity biotinylation, we captured and identified endogenous pro

293 Using APEX2 proximity biotinylation, we demonstrated that Rbd2 binds the AAA-A

294 tative protein mass spectrometry and surface biotinylation, we identified 100 proteins that showed si

295 Following surface biotinylation, Western blot analysis revealed full-lengt

296 were targeted to the cell surface by surface biotinylation/Western blots and confocal microscopy and

297 GLIB (glucosylation, periodate oxidation and biotinylation), which combines several enzymatic and che

298 Here, we visualized IPR by in vivo biotinylation, which revealed that the major IPR is a gr

299 Cell surface biotinylation with membrane-impermeable reagents and met

300 alyzed, spatially restricted in situ protein biotinylation with RNA-protein chemical crosslinking.