ライフサイエンスコーパス: bipolar spindle

1 ration is a prerequisite for positioning the bipolar spindle.

2 s on the organization of microtubules into a bipolar spindle.

3 ome segregation depends on the assembly of a bipolar spindle.

4 ntial in many species for the formation of a bipolar spindle.

5 ) and occurs before the establishment of the bipolar spindle.

6 y towards the poles following formation of a bipolar spindle.

7 ion, which in turn affect the formation of a bipolar spindle.

8 est at metaphase of meiosis II with a normal bipolar spindle.

9 cell cycle to ensure development of a normal bipolar spindle.

10 values cause a monaster to form instead of a bipolar spindle.

11 omplex that cannot establish or maintain the bipolar spindle.

12 centrosomes and thereby to the assembly of a bipolar spindle.

13 uclear envelope breakdown and formation of a bipolar spindle.

14 or the role of centrosomes in organizing the bipolar spindle.

15 ys that collaborate in the organization of a bipolar spindle.

16 ng and assembly of these microtubules into a bipolar spindle.

17 ides a force required for the formation of a bipolar spindle.

18 that are required for the establishment of a bipolar spindle.

19 les to the forces involved in formation of a bipolar spindle.

20 nd stabilize microtubules in order to form a bipolar spindle.

21 cultured mammalian cells does not require a bipolar spindle.

22 f chromosomes joined by a chiasma requires a bipolar spindle.

23 in, NCD, in tapering the microtubules into a bipolar spindle.

24 uring prophase these cells form a functional bipolar spindle.

25 ssential for the structural integrity of the bipolar spindle.

26 es progressively connects chromosomes to the bipolar spindle.

27 hin the nucleus to assemble and elongate the bipolar spindle.

28 s required for the formation of a functional bipolar spindle.

29 gle nucleus with 2C DNA content, and a short bipolar spindle.

30 or the formation and functioning of a normal bipolar spindle.

31 ive at coalescing extra spindle poles into a bipolar spindle.

32 tubules and the ability to form a simplified bipolar spindle.

33 clustering, is essential for formation of a bipolar spindle.

34 uired for the formation and maintenance of a bipolar spindle.

35 hromosome segregation is accomplished by the bipolar spindle.

36 of microtubule length within the assembling bipolar spindle.

37 osomes to separate and form the poles of the bipolar spindle.

38 te in an antiparallel orientation, forming a bipolar spindle.

39 n, although it was dispensable for forming a bipolar spindle.

40 opposite spindle to provide stability to the bipolar spindle.

41 man kinesin involved in the formation of the bipolar spindle.

42 in, and, ultimately, for formation of normal bipolar spindles.

43 ntil all sister kinetochores are attached to bipolar spindles.

44 to facilitate the transition from asters to bipolar spindles.

45 o-astral and monopolar structures instead of bipolar spindles.

46 protein that is required for organization of bipolar spindles.

47 ing spindle assembly blocks the formation of bipolar spindles.

48 rphase, enter mitosis normally, and assemble bipolar spindles.

49 rrest in G2 of the first cycle with complete bipolar spindles.

50 scapine arrest at mitosis with nearly normal bipolar spindles.

51 insert into the NM at meiosis I and nucleate bipolar spindles.

52 centrosomes could compromise the assembly of bipolar spindles.

53 r Ran on chromosomes, established functional bipolar spindles.

54 sis, centrosome maturation, and formation of bipolar spindles.

55 f mitotic cells with aligned chromosomes and bipolar spindles after dosing.

56 FZR1 controls the timing of assembly of the bipolar spindle and in so doing the timing of SAC satisf

57 ing cell division, the proper formation of a bipolar spindle and its function to segregate chromosome

58 a kinesin implicated in the formation of the bipolar spindle and its movement prior to and during ana

59 Plant cells assemble the bipolar spindle and phragmoplast microtubule (MT) arrays

60 t mediates Ran-GTP-dependent assembly of the bipolar spindle and promotes chromosome congression and

61 s during metaphase, after the formation of a bipolar spindle and the destruction of cyclin A, and it

62 ntrosome clustering mechanisms to assemble a bipolar spindle and to divide in a bipolar fashion.

63 mitotic fidelity, ensuring establishment of bipolar spindles and balanced chromosome segregation.

64 he ability of A-549 and Calu-1 cells to form bipolar spindles and caused formation of monoasteral spi

65 meiosis I which results in a failure to form bipolar spindles and divide nuclei.

66 aturation, for assembly and maintenance of a bipolar spindle, and for proper chromosome segregation d

67 oteins are required for the assembly of this bipolar spindle, and while the meiotic spindle lacks tra

68 tic events such as assembly and stability of bipolar spindles, and faithful chromosome segregation in

69 s results in delayed spindle assembly, fewer bipolar spindles, and the appearance of aberrant microtu

70 itosis by contributing to the formation of a bipolar spindle apparatus.

71 ends on the formation of a microtubule-based bipolar spindle apparatus.

72 nds on formation of a microtubule (MT)-based bipolar spindle apparatus.

73 urthermore, this elongation does not require bipolar spindle architecture or dynamic microtubules.

74 ngly, when just one centrosome is destroyed, bipolar spindles are also formed that contain one centro

75 Bipolar spindles are formed in the absence of AIR-1, but

76 XCTK2 is not required for maintenance of bipolar spindles, as antibody addition to preformed spin

77 Bipolar spindles assemble in the absence of centrosomes

78 ocyte meiotic cell division in many animals, bipolar spindles assemble in the absence of centrosomes,

79 without centrioles exhibited both a delay in bipolar spindle assembly and a high rate of chromosomal

80 osomal component of the spindle required for bipolar spindle assembly and function.

81 f microtubule-based motors, is essential for bipolar spindle assembly and maintenance during mitosis,

82 ration, indicating a role of SUN proteins in bipolar spindle assembly and mitotic progression.

83 tein phosphatase PP2A(Cdc55) activity blocks bipolar spindle assembly and nuclear divisions.

84 within the spindle midzone to play roles in bipolar spindle assembly and proper chromosome distribut

85 ked the ability of excess XCTK2 to stimulate bipolar spindle assembly and resulted in XCTK2-mediated

86 required for microtubule polymerization and bipolar spindle assembly around chromatin beads.

87 l cell cycle events of nuclear formation and bipolar spindle assembly around exogenously added sperm

88 yeast cells in mitosis at a stage following bipolar spindle assembly but prior to anaphase spindle e

89 Like Kif2a-deficient cells, bipolar spindle assembly can be restored to Kif2b-defici

90 drives efficient chromosome segregation and bipolar spindle assembly during mitosis.

91 s: first promoting centrosome separation and bipolar spindle assembly during prophase/prometaphase, a

92 eby paternal ZYG-1 regulates duplication and bipolar spindle assembly during the first cell cycle, an

93 re, this mechanism is strong enough to drive bipolar spindle assembly even in the presence of a singl

94 Bipolar spindle assembly in CDH1-m11 cells is strikingly

95 tides destabilizes microtubules and inhibits bipolar spindle assembly in HeLa cells.

96 the highly conserved ch-TOG gene to regulate bipolar spindle assembly in human cells.

97 ule sliding generated by Eg5 activity during bipolar spindle assembly in mammalian cells is regulated

98 spindle, which was subsequently required for bipolar spindle assembly in S phase.

99 microtubule-associated proteins required for bipolar spindle assembly in the absence of the centrosom

100 ads , AurA-coated beads increase the rate of bipolar spindle assembly in the presence of RanGTP, and

101 he microtubule assembly pathways compromises bipolar spindle assembly in tissue culture cells but not

102 ement in balancing Eg5-induced forces during bipolar spindle assembly in vitro and in vivo, and show

103 Bipolar spindle assembly is a critical control point for

104 Bipolar spindle assembly is critical for achieving accur

105 rosome, but, in its absence, the fidelity of bipolar spindle assembly is highly compromised.

106 le assembly pathways are integrated to drive bipolar spindle assembly is poorly understood.

107 edly, we found both microtubule assembly and bipolar spindle assembly required glycogen, which acted

108 Bipolar spindle assembly requires a balance of forces wh

109 Bipolar spindle assembly was restored in cells lacking K

110 rotation of the centrosome/nucleus complex, bipolar spindle assembly, anaphase chromosome segregatio

111 oles of mitotic spindles and is required for bipolar spindle assembly, but its molecular function in

112 ocity approximately 20%, but does not hinder bipolar spindle assembly, chromosome alignment, or mitot

113 microtubules and are instrumental in mitotic bipolar spindle assembly, ensuring orderly cell cycle pr

114 We show that four kinesins are involved in bipolar spindle assembly, four kinesins are involved in

115 /Thr-17-21 cluster was the most critical for bipolar spindle assembly, whereas other phospho-deficien

116 iated proteins (MAPs) is required for proper bipolar spindle assembly, yet the precise mechanisms by

117 ts suggest a mechanism for how Kif15 rescues bipolar spindle assembly.

118 leus at the onset of mitosis is critical for bipolar spindle assembly.

119 NP-O) and Fta1R(CENP-L)) causes a failure in bipolar spindle assembly.

120 e midzone-associated protein is required for bipolar spindle assembly.

121 o separate centrosomes, thus ensuring robust bipolar spindle assembly.

122 ting pericentriolar material cohesion during bipolar spindle assembly.

123 hat underlies both centrosome maturation and bipolar spindle assembly.

124 nd antagonizes MCAK activity, thus promoting bipolar spindle assembly.

125 Hice1 in a spatiotemporal manner for proper bipolar spindle assembly.

126 (MT) motor, pushes centrosomes apart during bipolar spindle assembly; its suppression results in mon

127 enerate antagonistic forces during mammalian bipolar spindle assembly; what remains unknown, however,

128 re-spindle misattachments and an increase in bipolar spindles associated with ectopic asters.

129 rosome during interphase, the formation of a bipolar spindle at mitosis and cytokinesis.

130 d with the control microspores, which formed bipolar spindles at the cell periphery, the mutant cells

131 ng cell division, microtubules that form the bipolar spindle attach to and pull on paired chromosome

132 ossover recombination into one that promotes bipolar spindle attachment and localized cohesion loss.

133 is centromere pairing mediates the meiosis I bipolar spindle attachment of nonexchange chromosome pai

134 s kinetochore structure, causes a failure of bipolar spindle attachment, and results in chromosome no

135 s the efficiency with which chromosomes make bipolar spindle attachments and regulates kinetochore ac

136 sts that they are unable to establish stable bipolar spindle attachments, presumably due to the inabi

137 s are connected to the opposite poles of the bipolar spindle ("bioriented").

138 show that the majority of cells establish a bipolar spindle but have defects in spindle orientation.

139 d-sensitive mitotic arrest with an elongated bipolar spindle but impaired anaphase A.

140 In the absence of AurA, cells form bipolar spindles but fail to properly align their chromo

141 duplicate and separate their SPBs to form a bipolar spindle, but spindle elongation and chromosome s

142 aphase spermatocytes were characterized with bipolar spindles, but chromosomes radiated away from the

143 ts enter mitosis at the normal time and form bipolar spindles, but fail chromosome alignment at the m

144 its ability to cluster centrosomes and form bipolar spindles, but it is not required for division in

145 Duo1p and Dam1p are not required to assemble bipolar spindles, but they are required to maintain meta

146 the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, i

147 Kinesin-5s help assemble the bipolar spindle by crosslinking and sliding apart antipa

148 study reveals that mouse oocytes assemble a bipolar spindle by fragmenting multiple acentriolar micr

149 KCBP promotes the formation of a converging bipolar spindle by sliding and bundling microtubules.

150 ng microtubules, but cells eventually formed bipolar spindles by an acentrosomal pole-focusing mechan

151 Hsp72, is required for assembly of a robust bipolar spindle capable of efficient chromosome congress

152 noscapine-arrested cells have nearly normal bipolar spindles, cells arrested by 5-Br-nosc and Rd 5-B

153 Time-lapse movies of GFP-labeled mono- and bipolar spindles demonstrate that KLP-19 generates a for

154 , the proper assembly of a microtubule-based bipolar spindle depends on signals from chromatin.

155 Imaging bipolar spindles disassembling in the presence of monast

156 e is important for spindle assembly, because bipolar spindles do not form in cells lacking centrosome

157 icates during the cell cycle and assembles a bipolar spindle during mitosis to capture and segregate

158 l role in orchestrating the formation of the bipolar spindle during mitosis.

159 Kinesin-5 is required for forming the bipolar spindle during mitosis.

160 nomic stability through the establishment of bipolar spindles during cell division, ensuring equal se

161 r protein, is essential for the formation of bipolar spindles during mitosis.

162 osomes clustered into two poles whose pseudo-bipolar spindles exhibit reduced fidelity of chromosome

163 When Fin1 is mislocalized, bipolar spindles fail to assemble but the spindle checkp

164 chromosomes are attached and oriented on the bipolar spindle for subsequent segregation at anaphase.

165 In the oocytes of many species, bipolar spindles form in the absence of centrosomes.

166 i-treated cells, we show that poorly focused bipolar spindles form through the self-organization of m

167 pair of centrioles separate, and functional bipolar spindles form with only one centriole at each sp

168 y (SPB) once per cell cycle is essential for bipolar spindle formation and accurate chromosome segreg

169 mitotic activity as evidenced by blockade of bipolar spindle formation and appearance of monoasters.

170 ough mitosis and tumor growth is by blocking bipolar spindle formation and chromosome alignment.

171 importance of alpha-N-methylation for normal bipolar spindle formation and chromosome segregation.

172 nterfering RNA (siRNA) and showed defects in bipolar spindle formation and cytokinesis, growth inhibi

173 plk1 Delta N resulted in repeated cycles of bipolar spindle formation and disruption, suggestive of

174 MT-based MT nucleation to accomplish normal bipolar spindle formation and mitotic progression.

175 dependent degradation of KIFC1 regulates the bipolar spindle formation and proper cell division.

176 ells at mitosis, likely due to the defect of bipolar spindle formation and subsequent activation of t

177 Centrosome separation, critical for bipolar spindle formation and subsequent chromosome segr

178 Centrosome separation is critical for bipolar spindle formation and the accurate segregation o

179 e extracts, Xkid and Xklp1 are essential for bipolar spindle formation but the functions of the human

180 omere positioning, microtubule dynamics, and bipolar spindle formation can all contribute to chromoso

181 he BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell divisio

182 kinase required for centrosome splitting and bipolar spindle formation during mitosis.

183 and Alp14/Dis1 play a collaborative role in bipolar spindle formation during prometaphase through pr

184 ator of centrosome duplication, required for bipolar spindle formation in HeLa human carcinoma cells

185 acentrosomal poles all contribute to robust bipolar spindle formation in meiotic extracts.

186 motor Klp61F, which is known for its role in bipolar spindle formation in mitosis, is required for pr

187 hat lead to timely centrosome separation and bipolar spindle formation in mitosis.

188 omal microtubule assembly pathway and favors bipolar spindle formation in most animal cells in which

189 on in the vicinity of chromosomes to mediate bipolar spindle formation in the absence of centrioles.

190 ucleation plays an important role for proper bipolar spindle formation in various eukaryotic organism

191 d is essential for centrosome separation and bipolar spindle formation in vitro and in vivo.

192 Kif15-dependent bipolar spindle formation in vivo requires the C-termina

193 Monastrol also inhibits bipolar spindle formation in Xenopus egg extracts.

194 Faithful chromosome segregation with bipolar spindle formation is critical for the maintenanc

195 Bipolar spindle formation is essential for the accurate

196 Bipolar spindle formation is pivotal for accurate segreg

197 Neither bipolar spindle formation nor centrosome functions were

198 to the spindles, thus resulting in improper bipolar spindle formation that ultimately leads to mitot

199 normal for assembly of the Ndc80 complex and bipolar spindle formation yet defective in proper end-on

200 ediate multiple mitotic processes, including bipolar spindle formation, activation of Cdc25C, actin r

201 omplex implicated in kinetochore attachment, bipolar spindle formation, and cytokinesis.

202 les, promotes microtubule polymerization and bipolar spindle formation, and decreases the turnover ra

203 or kinesin-5-mediated centrosome separation, bipolar spindle formation, and equal centrosome/centriol

204 e it regulates mitotic microtubule dynamics, bipolar spindle formation, and subsequent chromosome seg

205 tion of the PBD function results in improper bipolar spindle formation, chromosome missegregation, an

206 at Eg5 inhibition led to either monopolar or bipolar spindle formation, depending on whether centroso

207 rise from defects in centrosome duplication, bipolar spindle formation, kinetochore-microtubule attac

208 et of FTIs affecting centrosome position and bipolar spindle formation, likely explaining some of the

209 to allow stable K-MT attachments only after bipolar spindle formation, thus preventing attachment er

210 f Plk1 including (from low to high activity) bipolar spindle formation, timely mitotic entry, and for

211 ation site (S719), which positively enhances bipolar spindle formation.

212 and mitotic arrest with a profound defect in bipolar spindle formation.

213 ATPase activity and plays essential roles in bipolar spindle formation.

214 Plx1 functions on the centrosome to promote bipolar spindle formation.

215 and Cls1 activity are sufficient for initial bipolar spindle formation.

216 lp14 and Dis1 share an essential function in bipolar spindle formation.

217 ed assembly of microtubules is essential for bipolar spindle formation.

218 lyses have revealed that sub is required for bipolar spindle formation.

219 lp14 mutant fails to progress towards normal bipolar spindle formation.

220 ears to be defective in nuclear migration or bipolar spindle formation.

221 es centrosome separation and, in some cases, bipolar spindle formation.

222 a stimulation in both the rate and extent of bipolar spindle formation.

223 causes a 70% reduction in the percentage of bipolar spindles formed.

224 t not cin8-3 cells, were greatly impaired in bipolar spindle forming ability.

225 The bipolar spindle forms without centrosomes naturally in f

226 s separate to lie in distinct fenestrae as a bipolar spindle forms.

227 at metaphase I, unlike the typical fusiform bipolar spindle found in the wild-type metaphase I cells

228 cell-cycle regulation that is independent of bipolar spindle function.

229 Bipolar spindle fusion was blocked when cytoplasmic dyne

230 ssential role in mitosis by establishing the bipolar spindle, has proven to be an interesting drug ta

231 Establishing the bipolar spindle in mammalian oocytes after their prolong

232 erlies its role in supporting formation of a bipolar spindle in mitosis.

233 om cytoplasmic transport to formation of the bipolar spindle in mitosis.

234 spindle pole separation and the assembly of bipolar spindle in the absence of molecular motors.

235 n-like protein promotes assembly of a stable bipolar spindle in the absence of typical MTOCs.

236 Collapse of bipolar spindles in MCAK-deficient cells is driven by po

237 aughter cells could not assemble functional, bipolar spindles in the ensuing mitosis.

238 phatase resulted in the rapid disassembly of bipolar spindles into large asters.

239 , chromosome movement to the poles converted bipolar spindles into pairs of independent monopolar spi

240 Chromosome alignment in the middle of the bipolar spindle is a hallmark of metazoan cell divisions

241 Like other cellular organelles, the bipolar spindle is a structure of well-defined size and

242 ther in a polar body-like structure, while a bipolar spindle is established around the metaphase-arre

243 tic spindle, it remains unclear how a stable bipolar spindle is established.

244 taphase/anaphase transition to ensure that a bipolar spindle is formed and that all the chromosomes a

245 ny eukaryotic cells going through M-phase, a bipolar spindle is formed by microtubules nucleated from

246 As a bipolar spindle is formed, the median MT length increase

247 nesis of the microtubule cytoskeleton into a bipolar spindle is required for the faithful transmissio

248 In the mipAD123/klpA1 strain, formation of bipolar spindles is more strongly inhibited than in the

249 ly focused, diamond-shaped appearance of the bipolar spindle, K fibers need to be interconnected with

250 ntrast, and differing from previous reports, bipolar spindle length is relatively insensitive to incr

251 py-number plasmid R1 involves formation of a bipolar spindle made of left-handed double-helical actin

252 hout Eg5, whereas stabilizing K-MTs improves bipolar spindle maintenance without Eg5.

253 hed kinetochore-MTs (K-MTs) is important for bipolar spindle maintenance without Eg5.

254 Bipolar spindles must separate chromosomes by the approp

255 implicated in the formation and function of bipolar spindles on the basis of their respective locali

256 5 activity is dispensable for maintenance of bipolar spindles once they are formed [3, 4], suggesting

257 at the nuclear envelope before NEBD, and the bipolar spindle only emerged clearly after NEBD.

258 bl-deficient testes exhibit abnormalities in bipolar spindle organization, chromosome segregation, an

259 of p53 is not activated by abnormalities in bipolar spindle organization, chromosome segregation, ce

260 ding modules are required to rescue focused, bipolar spindle organization.

261 As the two centrosomes split to assemble the bipolar spindle, predominantly the old centrosome migrat

262 We propose that the bipolar spindle propagates its own architecture by stimu

263 aberrant mitosis despite normal assembly of bipolar spindles, resulting in either apoptosis or forma

264 ring cytokinesis in cells with monopolar and bipolar spindles shows that a subpopulation of stable mi

265 shows that when sliding is inhibited, short bipolar spindles still form, and if clustering is enhanc

266 upporting the formation of half-spindles and bipolar spindle structures around unreplicated chromosom

267 mouse oocytes, formation of the acentrosomal bipolar spindle takes 3-4 h, and stabilization of K-MT a

268 rotubule (MT) cytoskeleton rearranges into a bipolar spindle that drives chromosome segregation.

269 at mitosis depends on a correctly assembled bipolar spindle that exerts balanced forces on each sist

270 ht chromatin mass, and then bundle to form a bipolar spindle that lacks asters.

271 protein capable of promoting the assembly of bipolar spindles that do not include centrosomes or chro

272 minority of CDH1-m11 cells arrest with short bipolar spindles that fail to progress to anaphase; this

273 clustering of extra centrosomes into pseudo-bipolar spindles, thereby ensuring viable cell division.

274 tubules emanating from opposing sides of the bipolar spindle through large protein complexes called k

275 form the poles that direct the assembly of a bipolar spindle, thus ensuring the accurate segregation

276 simple two-motor model could create stable, bipolar spindles under a wide range of physical paramete

277 le (MT) plus ends from opposite poles of the bipolar spindle via kinetochores.

278 es of different motor proteins in building a bipolar spindle, we have used a simplified system in whi

279 cient cells are unique in that they assemble bipolar spindles when the pole focusing activities of Nu

280 m egg extracts yields weakened and elongated bipolar spindles which fail to align chromosomes.

281 task of the SPB is to ensure assembly of the bipolar spindle, which requires a proper balancing of fo

282 cle-treated control cells exhibited a normal bipolar spindle with chromosomes aligned along the metap

283 king S360 phosphorylation (S360D) results in bipolar spindles with a normal number of microtubules bu

284 Moderately affected embryos contained bipolar spindles with dense and long astral microtubule

285 such as multipolar spindles and asymmetric, bipolar spindles with lagging chromosomes.

286 , whereas metaphase cells exhibited aberrant bipolar spindles with Mad2 localization at kinetochores

287 Multipolar spindles and bipolar spindles with misaligned chromatin are also indu

288 Analysis of experimentally generated bipolar spindles with only one centrosome, as well as of

289 However, short bipolar spindles with organized poles formed after pertu

290 e slide-and-cluster mechanism robustly forms bipolar spindles with sharp poles and a stable steady-st

291 ear how multiple chromosomes form one shared bipolar spindle without centrosomes.

292 One is how to organize a bipolar spindle without microtubule organizing centers a

293 Cells that efficiently maintain bipolar spindles without Eg5 have more stable K-MTs than