ライフサイエンスコーパス: bisulfite

1 gy for large-scale mutational analysis (RESA-bisulfite).

2 ver, we demonstrate that Glut-3SH-Al and its bisulfite adduct are present in grape juice and could be

3 Ester and carbamate prodrugs of aldehyde bisulfite adduct inhibitors were synthesized in order to

4 t potency to those of the precursor aldehyde bisulfite adducts and precursor aldehydes.

5 ide applicability and can be extended to the bisulfite adducts of common warheads employed in the des

6 Methpat was used to analyse multiplex bisulfite amplicon sequencing on a range of CpG island t

7 ood differentially methylated regions, using bisulfite amplicon sequencing.

8 Reaction products of bisulfite and caftaric acid were found in wines containi

9 by parallel processing of genomic DNA using bisulfite and oxidative bisulfite conversion in conjunct

10 from paired DNA samples that have undergone bisulfite and oxidative bisulfite conversion.

11 xBS-MLE based on binomial modeling of paired bisulfite and oxidative bisulfite data from sequencing o

12 toma through parallel processing of DNA with bisulfite and oxidative bisulfite treatments.

13 Here, we used whole-genome bisulfite and transcriptome sequencing to characterize t

14 the reactions of sulfite (sulfite anions or bisulfite anions) with holes or hydroxyl radicals are th

15 sulfur species (RSS), including sulfite and bisulfite, as well as sulfane sulfur species and related

16 To resolve this issue, we developed a bisulfite-based approach, bisDRIP-seq, to map R-loops ac

17 more complete 5hmC landscape than available bisulfite-based methods.

18 age of the method, comprising cell lysis and bisulfite (BS) conversion, preamplification and adaptor

19 The use of sodium bisulfite (BS) treatment followed by hybridization to an

20 -glutathionyl caftaric acid, suggesting that bisulfite consistently competes as a nucleophile with gl

21 ine to support the comprehensive analysis of bisulfite conversion and immunoprecipitation-based methy

22 of genomic DNA followed by adapter ligation, bisulfite conversion and limited amplification using ada

23 ther with experimental parameters, including bisulfite conversion and oxidation efficiencies, as well

24 We measured percentage DNA methylation using bisulfite conversion and pyrosequencing assays on DNA fr

25 Using methylation-sensitive restriction and bisulfite conversion assays, we further showed that PSTV

26 or the dual use of 62 371 probes as internal bisulfite conversion controls.

27 Sodium bisulfite conversion followed by sequencing (BS-Seq, suc

28 of genomic DNA using bisulfite and oxidative bisulfite conversion in conjunction with RNA sequencing.

29 at single CpG dinucleotide resolution after bisulfite conversion of a target DNA sequence.

30 e report a method that combines TET-assisted bisulfite conversion with Illumina 450K DNA methylation

31 Coupling bisulfite conversion with next-generation sequencing (Bi

32 Gold-standard, widely used technologies (bisulfite conversion, followed by deep sequencing) canno

33 This sensitive bisulfite conversion-free method can be applied to biolo

34 that have undergone bisulfite and oxidative bisulfite conversion.

35 d amplicon, massively parallel sequencing of bisulfite converted DNA in a compact and interpretable f

36 gn with a unique best alignment score to the bisulfite converted reference mouse genome mm10.

37 set of 558 subjects using pyrosequencing of bisulfite-converted DNA (min P-value < 10(-30)).

38 We sequenced bisulfite-converted DNA from two tissues from each of tw

39 We present a capture-based approach for bisulfite-converted DNA that allows interrogation of pre

40 ing reads from high-throughput sequencing of bisulfite-converted DNA to reconstruct heterogeneous cel

41 articipants using targeted pyrosequencing of bisulfite-converted DNA.

42 VAliBS provides fast and accurate mapping of bisulfite-converted reads, and a friendly window system

43 etection programs, even while functioning on bisulfite-converted reads.

44 l modeling of paired bisulfite and oxidative bisulfite data from sequencing or array analysis.

45 a general approach for modeling whole-genome bisulfite data to identify differentially methylated sit

46 al TLR2 promoter methylation, as revealed by bisulfite DNA sequencing.

47 Here we present fC-CET, a bisulfite-free method for whole-genome analysis of 5fC b

48 essibility Protocol for individual templates-Bisulfite Genome Sequencing (MAPit-BGS) and nucleosome o

49 thyl sulfoxide-polymerase chain reaction and bisulfite genome sequencing; whereas, it was unmethylate

50 Here we performed whole-genome bisulfite, genome and transcriptome sequencing in 13 IG-

51 methylation of five genes was quantified by bisulfite genomic sequencing in d-200 dorsal prostates a

52 DNA methylation profiling and bisulfite genomic sequencing revealed that KLF4 expressi

53 t changing DNA methylation, when analyzed by bisulfite genomic sequencing.

54 -(hexanal)-glutathione (Glut-3SH-Al) and its bisulfite (Glut-3SH-SO3) adduct were identified in Sauvi

55 lve the treatment of genomic DNA with sodium bisulfite; however, this method cannot distinguish betwe

56 to an ability to bypass both DNA lesions and bisulfite intermediates, allowing significantly milder c

57 Seeker2), VAliBS can improve the accuracy of bisulfite mapping.

58 s within regulatory elements, we developed a bisulfite-mediated nucleotide-conversion strategy for la

59 ous T-cell lymphomas (CTCLs), using standard bisulfite modification techniques.

60 CA2/3) and CA1 postmortem human hippocampus, bisulfite modified it, and assessed it with the Infinium

61 histone methylation has been identified, we bisulfite-modified the extracted DNA and PCR-amplified 2

62 en demonstrated that paired BS and oxidative bisulfite (oxBS) treatment on the same sample followed b

63 In this study, we combine oxidative bisulfite (oxBS) treatment with the Illumina Infinium 45

64 methods (HhaI-assay and sequencing of cloned bisulfite PCR products).

65 (Benjamini-Hochberg) and verified hits using bisulfite PCR pyrosequencing and qPCR.

66 a or serum of donors, treated the cfDNA with bisulfite, PCR-amplified the cfDNA, and sequenced it to

67 Candidate genes were validated by bisulfite pyrosequencing (30 AGA, 21 SGA) and also analy

68 for DNA methylation and mRNA levels by using bisulfite pyrosequencing and quantitative RT-PCR in mono

69 from mouse cortex and performed quantitative bisulfite pyrosequencing at nine loci.

70 Targeted bisulfite pyrosequencing demonstrated that miR-34A was i

71 Independent verification by bisulfite pyrosequencing generally confirmed the percent

72 romoter methylation was measured by means of bisulfite pyrosequencing in patients.

73 owed that MeTIL markers can be determined by bisulfite pyrosequencing of small amounts of DNA from fo

74 Targeted bisulfite pyrosequencing was performed on a validation c

75 uantitative PCR), DNA methylation status (by bisulfite pyrosequencing), and GAL peptide by RIA of the

76 hylation-specific polymerase chain reaction, bisulfite pyrosequencing, and restriction enzyme-polymer

77 digest and ten-eleven translocation-assisted bisulfite pyrosequencing, to quantify FMR1 5mC and 5hmC

78 Findings were validated using bisulfite pyrosequencing.

79 methylated DMP was successfully validated by bisulfite-pyrosequencing, and identified DMPs were teste

80 AT_MM, of which the significant retention of bisulfite-resistant cytosines was corroborated by reanal

81 Combined bisulfite restriction analysis analysis of some of the t

82 ure of reduced PutA complexed with menadione bisulfite reveals the elusive quinone-binding site.

83 and systematically compared five widely used Bisulfite-seq mapping algorithms: Bismark, BSMAP, Pash,

84 However, despite a proliferation of Bisulfite-seq mapping tools, no systematic comparison of

85 conversion with next-generation sequencing (Bisulfite-seq) enables genome-wide measurement of DNA me

86 bisulfite sequencing (RRBS) and whole genome bisulfite sequencing (bis-seq) opens the door to study D

87 Bisulfite sequencing (BS-seq) has emerged recently as th

88 In standard bisulfite sequencing (BS-seq), unmodified C, 5fC and 5ca

89 methylation signals with similar accuracy as bisulfite sequencing (BS-Seq; single nucleotide resoluti

90 we present customised Reduced Representation Bisulfite Sequencing (cuRRBS), a novel and easy-to-use c

91 dy, we describe low-input methylase-assisted bisulfite sequencing (liMAB-seq ) and single-cell MAB-se

92 have recently developed a methylase-assisted bisulfite sequencing (MAB-seq) method that allows base-r

93 Here, we describe M.SssI methylase-assisted bisulfite sequencing (MAB-seq), a method that directly m

94 mic DNA, we combined redBS-Seq and oxidative bisulfite sequencing (oxBS-Seq) to generate the first co

95 Here we introduce reduced bisulfite sequencing (redBS-Seq), a quantitative method

96 Using both reduced representation bisulfite sequencing (RRBS) and microarray, we determine

97 g was performed using reduced representation bisulfite sequencing (RRBS) and RNA-sequencing (RNA-Seq)

98 e wide application of reduced representation bisulfite sequencing (RRBS) and whole genome bisulfite s

99 e apply our method to reduced representation bisulfite sequencing (RRBS) data from multiple regions o

100 raditional MspI-based Reduced Representation Bisulfite Sequencing (RRBS) protocol to all restriction

101 We used reduced representation bisulfite sequencing (RRBS) to profile DNA methylation i

102 lation differences by reduced representation bisulfite sequencing (RRBS), we determined that, over ti

103 We report a single-cell bisulfite sequencing (scBS-seq) method that can be used

104 me maps from single cells, using single-cell bisulfite sequencing (scBS-seq), allowing the quantitati

105 n patterns using single-cell, locus-specific bisulfite sequencing (SLBS).

106 Whole-genome bisulfite sequencing (WGBS) allows genome-wide DNA methy

107 Whole-genome bisulfite sequencing (WGBS) analysis revealed that Tet-m

108 Here, we performed whole-genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-seq)

109 igenetic energy landscapes from whole-genome bisulfite sequencing (WGBS) data that enable us to quant

110 Whole-genome bisulfite sequencing (WGBS) has emerged as the gold-stan

111 Whole-genome bisulfite sequencing (WGBS) is the gold standard for stu

112 Techniques like whole-genome bisulfite sequencing (WGBS) make it possible to determin

113 y process in A. mellifera using whole genome bisulfite sequencing (WGBS) method.

114 The cost of whole-genome bisulfite sequencing (WGBS) remains a bottleneck for man

115 Whole genome bisulfite sequencing (WGBS) revealed that integrin alpha

116 Recent developments in whole genome bisulfite sequencing (WGBS) technology have enabled geno

117 increased disease risk we used whole genome bisulfite sequencing (WGBS) to analyze changes in DNA me

118 methylated regions (DMRs) from whole-genome bisulfite sequencing (WGBS).

119 ecipitation, luciferase promoter assays, and bisulfite sequencing analysis of sites in proximity.

120 Bisulfite sequencing analysis revealed the premature pro

121 trained on 14 tissues with both whole genome bisulfite sequencing and 450K array data.

122 pecific CpG region in 9 colorectal tumors by bisulfite sequencing and apply a tumor development model

123 enetic mark, was investigated using targeted bisulfite sequencing and characterized at functional lev

124 genome-wide, we used reduced representation bisulfite sequencing and found an extensive reorganizati

125 Whole-genome bisulfite sequencing and H3K27Ac chromatin-immunoprecipi

126 ompares favorably with nucleotide-resolution bisulfite sequencing and has better predictive power wit

127 Low-coverage whole-genome bisulfite sequencing and high-coverage sequence-capture

128 lastic leukemias (B-ALLs) using whole-genome bisulfite sequencing and high-definition microarrays, al

129 MM), a plasma cell neoplasm, by whole-genome bisulfite sequencing and high-density arrays, we observe

130 cell differentiation program by whole-genome bisulfite sequencing and high-density microarrays.

131 ation measurements confirmed by whole genome bisulfite sequencing and offers a better balance between

132 Study of whole-genome bisulfite sequencing and reduced representation bisulfit

133 olution through methods such as whole-genome bisulfite sequencing and reduced representation bisulfit

134 re investigated using reduced representation bisulfite sequencing and RNA sequencing, respectively.

135 Using 17 whole-genome bisulfite sequencing and RNA-seq datasets from different

136 Reduced representation bisulfite sequencing and RNA-seq show that dCas9-SunTag-

137 examine promoter accessibility, we performed bisulfite sequencing and show that none of the MHC class

138 elity, we implemented a genome-scale hairpin bisulfite sequencing approach to generate methylation da

139 With the genome-scale hairpin bisulfite sequencing approach, we demonstrated that the

140 nt a method to map DNA methylation data from bisulfite sequencing approaches to CpG sites measured wi

141 lation modifications from any combination of bisulfite sequencing approaches, including reduced, oxid

142 ulfite sequencing and reduced representation bisulfite sequencing brings the availability of DNA meth

143 HSD11B2 and FKBP5 are seen in a minority of bisulfite sequencing clones, these epigenetic changes, a

144 Bisulfite sequencing confirmed dense promoter hypermethy

145 Bisulfite sequencing confirmed hypermethylation of the A

146 Whole-genome bisulfite sequencing currently provides the highest-prec

147 ient and convenient tool for high-throughput bisulfite sequencing data analysis that can be broadly u

148 We generate whole-genome bisulfite sequencing data for approximately 30 adipose a

149 nalysis of previously published whole-genome bisulfite sequencing data for intragonadal PGCs.

150 By analyzing whole-genome bisulfite sequencing data in a phylogenetic context, it

151 ute deviations support the validity of using bisulfite sequencing data in combination with Illumina b

152 d validation with 101 reduced-representation bisulfite sequencing data sets and 637 methylation array

153 pe blocks, after analysis of 61 whole-genome bisulfite sequencing data sets and validation with 101 r

154 Analysis of bisulfite sequencing data usually requires two tasks: to

155 d coverage depth in the case of whole-genome bisulfite sequencing data).

156 h results correlating well with whole genome bisulfite sequencing data, and demonstrate that human DN

157 tus and DNA polymorphisms, from whole-genome bisulfite sequencing data, and nucleosome occupancy from

158 on analysis of DNA methylation patterns from bisulfite sequencing data, including the detection of re

159 Using high resolution hairpin oxidative bisulfite sequencing data, we precisely determine the am

160 odification assisted, and methylase-assisted bisulfite sequencing data.

161 entify bipolar methylated genomic regions in bisulfite sequencing data.

162 arrying out such analysis on high-throughput bisulfite sequencing data.

163 on studies have generated massive amounts of bisulfite sequencing data.

164 ware package for detecting mCs and DMRs from bisulfite sequencing data.

165 By utilizing a real bisulfite sequencing dataset generated from prostate can

166 ally methylated regions from high-throughput bisulfite sequencing datasets, DMRfinder is the first th

167 Bisulfite sequencing evaluated DNA methylation of placen

168 The analysis of multiple whole-genome bisulfite sequencing experiments is supported, while mai

169 lyses of datasets from RNA-Seq, ChIP-Seq and bisulfite sequencing experiments.

170 Furthermore, whole-genome bisulfite sequencing failed to reveal any evidence of de

171 Here, we used RNA bisulfite sequencing for transcriptome-wide quantitative

172 Here we used whole-genome bisulfite sequencing from 10 diverse human tissues to id

173 e information from whole genome and targeted bisulfite sequencing from 910 samples to perform genotyp

174 Bisulfite sequencing further reveals methylation of the

175 Conventional DNA bisulfite sequencing has been extended to single cell le

176 We used whole-genome bisulfite sequencing in a mouse model with nonrandom XCI

177 hlights the utility of low pass whole-genome bisulfite sequencing in identifying methylome variation

178 tastable epialleles by performing genomewide bisulfite sequencing in peripheral blood lymphocyte (PBL

179 he human embryo and germ cells, and targeted bisulfite sequencing in term placentas.

180 s, publicly available reduced representation bisulfite sequencing in the human embryo and germ cells,

181 However, the standard workflow of bisulfite sequencing involves heat and strongly basic co

182 Bisulfite sequencing is a key methodology in epigenetics

183 Bisulfite sequencing is one of the most widely used tech

184 by the Illumina 450K array and whole genome bisulfite sequencing is still too expensive for many sam

185 ity within tumors, we performed genome-scale bisulfite sequencing of 104 primary chronic lymphocytic

186 ystem, we performed RNA-seq and whole-genome bisulfite sequencing of adult females and males from two

187 Whole-genome bisulfite sequencing of antigen-specific murine CD8 T ce

188 ethylation profile of the same CpG-island by bisulfite sequencing of DNA obtained from blood of 34 FT

189 Bisulfite sequencing of exposed and control animals high

190 lower KLF4 and nitric oxide synthase 3, and bisulfite sequencing of KLF4 promoter identified a hyper

191 Using whole-genome bisulfite sequencing of normal B cell subsets, we observ

192 Whole-genome bisulfite sequencing of primary human naive, short-lived

193 to leukemogenesis, we performed whole-genome bisulfite sequencing of primary leukemic and non-leukemi

194 By employing a protocol for whole-genome bisulfite sequencing of single cells, we show that the l

195 Bisulfite sequencing of sperm DNA from conditioned F0 ma

196 We performed bisulfite sequencing on 23 CpG dinucleotides on the tran

197 Other studies have performed whole-genome bisulfite sequencing on a few individuals, but these lac

198 We perform whole genome bisulfite sequencing on a set of unmatched samples inclu

199 To illustrate MCC-Seq, we use whole-genome bisulfite sequencing on adipose tissue (AT) samples and

200 in social insects, we performed whole-genome bisulfite sequencing on brains of the clonal raider ant

201 Asthma Study [n = 28]) was analyzed by using bisulfite sequencing or Illumina 450K arrays.

202 by sequencing (BS-Seq, such as whole genome bisulfite sequencing or reduced representation bisulfite

203 have developed BSPAT, a fast online tool for bisulfite sequencing pattern analysis.

204 ition, we validated the MeDIP-Seq results by bisulfite sequencing PCR (BSP) in some of the differenti

205 We performed bisulfite sequencing PCR of genomic DNA isolated from HB

206 g of short reads by and high cost of current bisulfite sequencing platforms make them impractical for

207 Interestingly, additional RNA bisulfite sequencing provided first evidence for Dnmt2-m

208 Although Whole genome Bisulfite Sequencing provides high-quality methylation m

209 Chromatin immunoprecipitation and bisulfite sequencing quantified epigenetic characteristi

210 an automated analysis toolkit for processing bisulfite sequencing reads.

211 Bisulfite sequencing revealed no DNA-methylation in this

212 Bisulfite sequencing revealed that both human and mouse

213 Further Bisulfite sequencing revealed that miR-210 is embedded i

214 Time-series whole genome bisulfite sequencing reveals extensive gain of CHH methy

215 Targeted and whole-genome bisulfite sequencing showed that the induced ripening of

216 Here, we have analyzed multiple bisulfite sequencing studies to address the methylation

217 s hypothesis, we used reduced representation bisulfite sequencing to examine the cross-sectional geno

218 Using whole genome bisulfite sequencing to examine uncharted regions of the

219 Here we applied oxidative bisulfite sequencing to generate whole-genome DNA methyl

220 microdissection with reduced representation bisulfite sequencing to identify cancer-associated DNA m

221 we employed Enhanced Reduced Representation Bisulfite Sequencing to interrogate the epigenome of the

222 A recent study used genome-wide bisulfite sequencing to survey differences in DNA methyl

223 Currently available bisulfite sequencing tools frequently suffer from low ma

224 g (n = 12 per group), unbiased capture array bisulfite sequencing was combined with subsequent matrix

225 lation and hydroxymethylation with oxidative bisulfite sequencing was conducted and correlated with c

226 Whole-genome bisulfite sequencing was conducted to assess the dynamic

227 Targeted bisulfite sequencing was performed with a subset of plac

228 Reduced representational bisulfite sequencing was then used to compare the differ

229 Finally, oxidative bisulfite sequencing was used to differentiate methylati

230 Whole-genome bisulfite sequencing was used to produce nucleotide reso

231 By using methylation-specific PCR and bisulfite sequencing we demonstrate that miR-663 promote

232 Using whole genome bisulfite sequencing we identified hundreds of different

233 equencing and high-coverage sequence-capture bisulfite sequencing were applied to mutant lines to det

234 RNA-Seq and multiplex bisulfite sequencing were performed to examine gene expr

235 RNA sequencing and reduced representation bisulfite sequencing were used to create transcriptomic

236 rporation of the 5D4 DNA polymerase into the bisulfite sequencing workflow thus promises significant

237 sulfite sequencing or reduced representation bisulfite sequencing) has become popular for studying hu

238 We use whole genome bisulfite sequencing, and find that differentiation of m

239 efficient (R = 0.884) between our method and bisulfite sequencing, and for 92.0% of CpG sites, methyl

240 replicate these findings using whole genome bisulfite sequencing, comparing epidermis from an additi

241 ifications, including but not limited to RNA bisulfite sequencing, m(1)A-Seq, Par-CLIP, RIP-Seq, etc.

242 ular biology and optical approaches, such as bisulfite sequencing, microarrays, quantitative real-tim

243 sequencing cost in the case of whole-genome bisulfite sequencing, or from reduced resolution (inabil

244 sign, particularly in reduced representation bisulfite sequencing, there is a need to develop more fl

245 Using targeted bisulfite sequencing, we examined methylation of 2100 ge

246 By performing genome-scale bisulfite sequencing, we find that DNMT3B-deficient iPSC

247 Here, using high-throughput bisulfite sequencing, we identified an APL-associated hy

248 Using whole-genome bisulfite sequencing, we show that crossover remodeling

249 Through whole-genome bisulfite sequencing, we showed that DNA methylation wen

250 e increases in methylation were validated by bisulfite sequencing, where they occurred in a minority

251 Here, we performed whole-genome bisulfite sequencing, which is a comprehensive and unbia

252 e used whole-genome sequencing, whole-genome bisulfite sequencing, whole transcriptome (RNA-seq) and

253 ned using single cell reduced representation bisulfite sequencing, with a 66-fold increase in the fra

254 y the pattern of CpG methylation revealed by bisulfite sequencing.

255 ylation to nearby CpG sites, shown by sodium bisulfite sequencing.

256 m HumanMethylation450 array and whole genome bisulfite sequencing.

257 ATAC sequencing and single-cell whole-genome bisulfite sequencing.

258 ation landscape, as assessed by whole-genome bisulfite sequencing.

259 ulfite sequencing and reduced representation bisulfite sequencing.

260 Array findings were validated by bisulfite sequencing.

261 s of genomic DNA followed by next generation bisulfite sequencing.

262 uman aorta sample using whole-genome shotgun bisulfite sequencing.

263 idated using enhanced reduced representation bisulfite sequencing.

264 er hypermethylation of ULK2 was confirmed by bisulfite sequencing.

265 DNMT3L, shown by knockdown assays and sodium bisulfite sequencing.

266 genes was analyzed using 450K Beadchips and bisulfite sequencing; correlations between maternal and

267 Here, we present 4mC-Tet-assisted bisulfite-sequencing (4mC-TAB-seq), a next-generation se

268 region (-186 to -20), which was confirmed by bisulfite-sequencing and methylation-specific PCR (MSP)

269 a mixture of binomial model to characterize bisulfite-sequencing data, and based on the model, we pr

270 dealing with the various sources of noise in bisulfite-sequencing data.

271 However, analysis of data produced by bisulfite-sequencing poses statistical challenges owing

272 The rapid emergence of bisulfite-sequencing technologies enables performing suc

273 We used replicated bisulfite-sequencing to investigate patterns of DNA meth

274 dresses statistical challenges introduced by bisulfite-sequencing while controlling for complex sourc

275 nt of high-throughput sequencing technology, bisulfite-sequencing-based DNA methylation profiling met

276 arose due to experimental limitations of the bisulfite technique.

277 simply monitoring the relative adsorption of bisulfite treated DNA sequences onto a gold chip.

278 esign and validation of PCR amplification on bisulfite-treated DNA and pyrosequencing assays as well

279 Both can lead to poor recovery of bisulfite-treated DNA by the polymerase chain reaction (

280 nhanced ability to replicate and PCR amplify bisulfite-treated DNA due to an ability to bypass both D

281 We present a novel approach in which bisulfite-treated DNA from many individuals is sequenced

282 t direct DSN activity to multiple targets in bisulfite-treated DNA, simultaneously.

283 ased sensitivity in the PCR amplification of bisulfite-treated DNA.

284 analysis and by sequencing of PCR-amplified bisulfite-treated DNA.

285 affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads

286 plementation of the mapping tool BRAT-BW for bisulfite-treated reads (BS-Seq).

287 tional aligners are not designed for mapping bisulfite-treated reads, where the un-methylated 'C's ar

288 Here, we use sodium bisulfite treatment and targeted gene enrichment to stud

289 s either damaged DNA and/or intermediates of bisulfite treatment are poor substrate for standard DNA

290 is studied at a single-base resolution with bisulfite treatment followed by high-throughput sequenci

291 ns of the C/T polymorphism induced by sodium bisulfite treatment of DNA reflects the DNA methylation

292 BiSeqS employs bisulfite treatment to distinguish the two strands of mo

293 The three main strategies as (i) bisulfite treatment, (ii) cleavage by restriction endonu

294 Because of the special process with bisulfite treatment, traditional mapping tools do not wo

295 ngle-cell protocol based on agarose-embedded bisulfite treatment, which allows investigating DNA meth

296 hemical reduction of 5fC to 5hmC followed by bisulfite treatment.

297 DNA methylation detection approaches require bisulfite treatments and are laborious or costly to perf

298 ocessing of DNA with bisulfite and oxidative bisulfite treatments.

299 The alternative is to use whole genome bisulfite (WGB) sequencing but this approach is not yet

300 -sulfonate (dhU6S) (generated by reaction of bisulfite with deoxycytidine (dC)) to uracil (dU).