ライフサイエンスコーパス: bisulfite sequencing

1 m HumanMethylation450 array and whole genome bisulfite sequencing.

2 ATAC sequencing and single-cell whole-genome bisulfite sequencing.

3 ation landscape, as assessed by whole-genome bisulfite sequencing.

4 ulfite sequencing and reduced representation bisulfite sequencing.

5 Array findings were validated by bisulfite sequencing.

6 s of genomic DNA followed by next generation bisulfite sequencing.

7 uman aorta sample using whole-genome shotgun bisulfite sequencing.

8 idated using enhanced reduced representation bisulfite sequencing.

9 er hypermethylation of ULK2 was confirmed by bisulfite sequencing.

10 ith validated results than does whole-genome bisulfite sequencing.

11 er an Illumina methylation array or targeted bisulfite sequencing.

12 ASM) in data from high-throughput short-read bisulfite sequencing.

13 nstruction ("tagmentation") for whole-genome bisulfite sequencing.

14 he human genome for targeted high-throughput bisulfite sequencing.

15 tterns using microarrays, with validation by bisulfite sequencing.

16 ripotent and adult cell lines using Illumina bisulfite sequencing.

17 lation at 2 CpG-rich regions was measured by bisulfite sequencing.

18 DNMT3L, shown by knockdown assays and sodium bisulfite sequencing.

19 y the pattern of CpG methylation revealed by bisulfite sequencing.

20 ylation to nearby CpG sites, shown by sodium bisulfite sequencing.

21 Here, we present 4mC-Tet-assisted bisulfite-sequencing (4mC-TAB-seq), a next-generation se

22 Bisulfite sequencing allows cytosine methylation, an imp

23 Bisulfite sequencing analysis and ex vivo DNA methyltran

24 ecipitation, luciferase promoter assays, and bisulfite sequencing analysis of sites in proximity.

25 Bisulfite sequencing analysis of the transposable elemen

26 Bisulfite sequencing analysis revealed the premature pro

27 trained on 14 tissues with both whole genome bisulfite sequencing and 450K array data.

28 pecific CpG region in 9 colorectal tumors by bisulfite sequencing and apply a tumor development model

29 enetic mark, was investigated using targeted bisulfite sequencing and characterized at functional lev

30 Bisulfite sequencing and chromatin immunoprecipitation (

31 Current epigenomic analyses employ bisulfite sequencing and chromatin immunoprecipitation,

32 i (762 CpGs) for confirmation with PCR-based bisulfite sequencing and demonstrated excellent correlat

33 genome-wide, we used reduced representation bisulfite sequencing and found an extensive reorganizati

34 Whole-genome bisulfite sequencing and H3K27Ac chromatin-immunoprecipi

35 ompares favorably with nucleotide-resolution bisulfite sequencing and has better predictive power wit

36 Low-coverage whole-genome bisulfite sequencing and high-coverage sequence-capture

37 lastic leukemias (B-ALLs) using whole-genome bisulfite sequencing and high-definition microarrays, al

38 MM), a plasma cell neoplasm, by whole-genome bisulfite sequencing and high-density arrays, we observe

39 cell differentiation program by whole-genome bisulfite sequencing and high-density microarrays.

40 eptor alpha (RORA), was further confirmed by bisulfite sequencing and methylation-specific PCR, respe

41 ation measurements confirmed by whole genome bisulfite sequencing and offers a better balance between

42 olution through methods such as whole-genome bisulfite sequencing and reduced representation bisulfit

43 Study of whole-genome bisulfite sequencing and reduced representation bisulfit

44 re investigated using reduced representation bisulfite sequencing and RNA sequencing, respectively.

45 Using 17 whole-genome bisulfite sequencing and RNA-seq datasets from different

46 Reduced representation bisulfite sequencing and RNA-seq show that dCas9-SunTag-

47 We used deep single molecule bisulfite sequencing and sample-specific DNA barcodes to

48 examine promoter accessibility, we performed bisulfite sequencing and show that none of the MHC class

49 counts from bisulfite sequencing, oxidative bisulfite sequencing and Tet-Assisted Bisulfite sequenci

50 region (-186 to -20), which was confirmed by bisulfite-sequencing and methylation-specific PCR (MSP)

51 We use whole genome bisulfite sequencing, and find that differentiation of m

52 efficient (R = 0.884) between our method and bisulfite sequencing, and for 92.0% of CpG sites, methyl

53 ependymomas, we exploited a high-throughput bisulfite sequencing approach that simultaneously genera

54 We demonstrated the targeted bisulfite sequencing approach to be a powerful method to

55 elity, we implemented a genome-scale hairpin bisulfite sequencing approach to generate methylation da

56 With the genome-scale hairpin bisulfite sequencing approach, we demonstrated that the

57 nt a method to map DNA methylation data from bisulfite sequencing approaches to CpG sites measured wi

58 lation modifications from any combination of bisulfite sequencing approaches, including reduced, oxid

59 nt of high-throughput sequencing technology, bisulfite-sequencing-based DNA methylation profiling met

60 bisulfite sequencing (RRBS) and whole genome bisulfite sequencing (bis-seq) opens the door to study D

61 We used whole-genome bisulfite sequencing (bisulfite-seq) to comprehensively

62 ulfite sequencing and reduced representation bisulfite sequencing brings the availability of DNA meth

63 We have carried out whole-genome bisulfite sequencing (BS-Seq) and RNA-Seq across key sta

64 Bisulfite sequencing (BS-seq) has emerged as the gold st

65 Bisulfite sequencing (BS-seq) has emerged recently as th

66 Here we used shotgun genomic bisulfite sequencing (BS-Seq) to compare DNA methylation

67 In bisulfite sequencing (BS-seq), both 5mC and 5hmC are rea

68 In standard bisulfite sequencing (BS-seq), unmodified C, 5fC and 5ca

69 methylation signals with similar accuracy as bisulfite sequencing (BS-Seq; single nucleotide resoluti

70 to require combining these experiments with bisulfite sequencing, but doing so naively produces inco

71 we present a chemical modification-assisted bisulfite sequencing (CAB-Seq) that can detect 5caC with

72 AB-Seq), that when combined with traditional bisulfite sequencing can be used for mapping 5hmC at bas

73 g methyl-sensitive restriction digestion and bisulfite sequencing cannot distinguish between 5-mC and

74 Integration of whole-genome bisulfite sequencing, chromatin immunoprecipitation sequ

75 HSD11B2 and FKBP5 are seen in a minority of bisulfite sequencing clones, these epigenetic changes, a

76 replicate these findings using whole genome bisulfite sequencing, comparing epidermis from an additi

77 Bisulfite sequencing confirmed dense promoter hypermethy

78 Bisulfite sequencing confirmed hypermethylation of the A

79 Bisulfite sequencing confirmed that 14 genes were hyperm

80 genes was analyzed using 450K Beadchips and bisulfite sequencing; correlations between maternal and

81 we present customised Reduced Representation Bisulfite Sequencing (cuRRBS), a novel and easy-to-use c

82 Whole-genome bisulfite sequencing currently provides the highest-prec

83 t-generation Universal MAPper to accommodate bisulfite sequencing data (GNUMAP-bs), that addresses th

84 ient and convenient tool for high-throughput bisulfite sequencing data analysis that can be broadly u

85 dditional content selected from whole-genome bisulfite sequencing data and input from DNA methylation

86 We generate whole-genome bisulfite sequencing data for approximately 30 adipose a

87 nalysis of previously published whole-genome bisulfite sequencing data for intragonadal PGCs.

88 egration of these DNase I cleavage data with bisulfite sequencing data for the same cell type's genom

89 We generated bisulfite sequencing data from two tissues of Brachypodi

90 By analyzing whole-genome bisulfite sequencing data in a phylogenetic context, it

91 ute deviations support the validity of using bisulfite sequencing data in combination with Illumina b

92 Bisulfite sequencing data indicate that active nucleolar

93 Comparison with massively parallel bisulfite sequencing data indicates that 41% of variable

94 d validation with 101 reduced-representation bisulfite sequencing data sets and 637 methylation array

95 pe blocks, after analysis of 61 whole-genome bisulfite sequencing data sets and validation with 101 r

96 116 cells and analysis of genome-wide sodium bisulfite sequencing data sets from several other DNA so

97 putational problems associated with aligning bisulfite sequencing data to a reference genome.

98 Analysis of bisulfite sequencing data usually requires two tasks: to

99 Here, we combined whole-genome bisulfite sequencing data with extensive gene expression

100 d coverage depth in the case of whole-genome bisulfite sequencing data).

101 h results correlating well with whole genome bisulfite sequencing data, and demonstrate that human DN

102 tus and DNA polymorphisms, from whole-genome bisulfite sequencing data, and nucleosome occupancy from

103 on analysis of DNA methylation patterns from bisulfite sequencing data, including the detection of re

104 Using high resolution hairpin oxidative bisulfite sequencing data, we precisely determine the am

105 This conclusion is supported by bisulfite sequencing data, which shows only limited conv

106 entify bipolar methylated genomic regions in bisulfite sequencing data.

107 arrying out such analysis on high-throughput bisulfite sequencing data.

108 on studies have generated massive amounts of bisulfite sequencing data.

109 ware package for detecting mCs and DMRs from bisulfite sequencing data.

110 odification assisted, and methylase-assisted bisulfite sequencing data.

111 a mixture of binomial model to characterize bisulfite-sequencing data, and based on the model, we pr

112 dealing with the various sources of noise in bisulfite-sequencing data.

113 By utilizing a real bisulfite sequencing dataset generated from prostate can

114 ally methylated regions from high-throughput bisulfite sequencing datasets, DMRfinder is the first th

115 Genomic bisulfite sequencing demonstrates selective hypermethyla

116 Bisulfite sequencing evaluated DNA methylation of placen

117 Furthermore, bisulfite sequencing experiments have the additional com

118 The analysis of multiple whole-genome bisulfite sequencing experiments is supported, while mai

119 gnment approach for processing the data from bisulfite sequencing experiments.

120 lyses of datasets from RNA-Seq, ChIP-Seq and bisulfite sequencing experiments.

121 Furthermore, whole-genome bisulfite sequencing failed to reveal any evidence of de

122 reening promoter regions for CpG islands and bisulfite sequencing followed by QUMSP and RT PCR for th

123 We optimized bisulfite sequencing for genome-scale analysis of clinic

124 Here, we used RNA bisulfite sequencing for transcriptome-wide quantitative

125 Here we used whole-genome bisulfite sequencing from 10 diverse human tissues to id

126 e information from whole genome and targeted bisulfite sequencing from 910 samples to perform genotyp

127 venting the platform's potential utility for bisulfite sequencing from being realized.

128 Bisulfite sequencing further reveals methylation of the

129 Conventional DNA bisulfite sequencing has been extended to single cell le

130 sulfite sequencing or reduced representation bisulfite sequencing) has become popular for studying hu

131 We used whole-genome bisulfite sequencing in a mouse model with nonrandom XCI

132 ), a risky methylated gene, was confirmed by bisulfite sequencing in GBMs.

133 hlights the utility of low pass whole-genome bisulfite sequencing in identifying methylome variation

134 tastable epialleles by performing genomewide bisulfite sequencing in peripheral blood lymphocyte (PBL

135 he human embryo and germ cells, and targeted bisulfite sequencing in term placentas.

136 s, publicly available reduced representation bisulfite sequencing in the human embryo and germ cells,

137 However, the standard workflow of bisulfite sequencing involves heat and strongly basic co

138 Bisulfite sequencing is a key methodology in epigenetics

139 Bisulfite sequencing is a widely-used technique for exam

140 Bisulfite sequencing is one of the most widely used tech

141 by the Illumina 450K array and whole genome bisulfite sequencing is still too expensive for many sam

142 Bisulfite sequencing is the most direct and highest reso

143 pture microdissection-reduced representation bisulfite sequencing (LCM-RRBS) for the multiplexed inte

144 tocols, such that we generate highly complex bisulfite sequencing libraries from as little as 10 ng o

145 dy, we describe low-input methylase-assisted bisulfite sequencing (liMAB-seq ) and single-cell MAB-se

146 ifications, including but not limited to RNA bisulfite sequencing, m(1)A-Seq, Par-CLIP, RIP-Seq, etc.

147 have recently developed a methylase-assisted bisulfite sequencing (MAB-seq) method that allows base-r

148 Here, we describe M.SssI methylase-assisted bisulfite sequencing (MAB-seq), a method that directly m

149 cytosine DNA methyltransferases followed by bisulfite sequencing (MAPit) is an effective technique f

150 Bisulfite sequencing measures absolute levels of DNA met

151 Here we describe a modified bisulfite-sequencing method, Tet-assisted bisulfite sequ

152 fraction (<10%) of the cost of whole-genome bisulfite sequencing methods.

153 methods of DNA methylation analysis, such as bisulfite sequencing, methylation sensitive restriction

154 ular biology and optical approaches, such as bisulfite sequencing, microarrays, quantitative real-tim

155 ity within tumors, we performed genome-scale bisulfite sequencing of 104 primary chronic lymphocytic

156 Microarray-based screening and bisulfite sequencing of 20 nonmalignant and 29 PC tissue

157 as evidenced by the wide application of the bisulfite sequencing of 5-methylcytosine and very recent

158 ethylation states assessed from whole-genome bisulfite sequencing of 83 RILs uncovered widespread evi

159 ystem, we performed RNA-seq and whole-genome bisulfite sequencing of adult females and males from two

160 Whole-genome bisulfite sequencing of antigen-specific murine CD8 T ce

161 ethylation profile of the same CpG-island by bisulfite sequencing of DNA obtained from blood of 34 FT

162 Bisulfite sequencing of exposed and control animals high

163 Bisulfite sequencing of genomic DNA revealed two roughly

164 lower KLF4 and nitric oxide synthase 3, and bisulfite sequencing of KLF4 promoter identified a hyper

165 Using whole-genome bisulfite sequencing of normal B cell subsets, we observ

166 Whole-genome bisulfite sequencing of primary human naive, short-lived

167 to leukemogenesis, we performed whole-genome bisulfite sequencing of primary leukemic and non-leukemi

168 By employing a protocol for whole-genome bisulfite sequencing of single cells, we show that the l

169 Bisulfite sequencing of sperm DNA from conditioned F0 ma

170 Bisulfite sequencing of the prostaglandin E receptor 2 g

171 Bisulfite sequencing of the WIF-1 promoter region in RCC

172 me comparative view of DNA methylation using bisulfite sequencing of three cultured cell types repres

173 Targeted bisulfite sequencing of three DNA methyltransferase (DNM

174 We performed bisulfite sequencing on 23 CpG dinucleotides on the tran

175 Other studies have performed whole-genome bisulfite sequencing on a few individuals, but these lac

176 We perform whole genome bisulfite sequencing on a set of unmatched samples inclu

177 To illustrate MCC-Seq, we use whole-genome bisulfite sequencing on adipose tissue (AT) samples and

178 in social insects, we performed whole-genome bisulfite sequencing on brains of the clonal raider ant

179 We performed whole-genome bisulfite sequencing on fruit in four stages of developm

180 ic number of sequencing errors, facilitating bisulfite sequencing on this platform.

181 Asthma Study [n = 28]) was analyzed by using bisulfite sequencing or Illumina 450K arrays.

182 and rapid, accurate and detailed analysis of bisulfite sequencing or MAPit datasets from virtually an

183 moter and enhancer regions was determined by bisulfite sequencing or pyrosequencing.

184 by sequencing (BS-Seq, such as whole genome bisulfite sequencing or reduced representation bisulfite

185 sequencing cost in the case of whole-genome bisulfite sequencing, or from reduced resolution (inabil

186 mic DNA, we combined redBS-Seq and oxidative bisulfite sequencing (oxBS-Seq) to generate the first co

187 We introduce oxidative bisulfite sequencing (oxBS-Seq), the first method for qu

188 To this end, we have developed oxidative bisulfite sequencing (oxBS-seq), which can quantitativel

189 t a method to jointly model read counts from bisulfite sequencing, oxidative bisulfite sequencing and

190 have developed BSPAT, a fast online tool for bisulfite sequencing pattern analysis.

191 ition, we validated the MeDIP-Seq results by bisulfite sequencing PCR (BSP) in some of the differenti

192 We performed bisulfite sequencing PCR of genomic DNA isolated from HB

193 s, RRBS, WGBS sequencing, and locus-specific bisulfite sequencing performed on the same human embryon

194 g of short reads by and high cost of current bisulfite sequencing platforms make them impractical for

195 However, analysis of data produced by bisulfite-sequencing poses statistical challenges owing

196 Interestingly, additional RNA bisulfite sequencing provided first evidence for Dnmt2-m

197 Although Whole genome Bisulfite Sequencing provides high-quality methylation m

198 dative bisulfite sequencing and Tet-Assisted Bisulfite sequencing, providing simultaneous estimates o

199 Chromatin immunoprecipitation and bisulfite sequencing quantified epigenetic characteristi

200 an automated analysis toolkit for processing bisulfite sequencing reads.

201 Here we introduce reduced bisulfite sequencing (redBS-Seq), a quantitative method

202 High-quality bisulfite sequencing results were obtained for both gene

203 ram, called MethylViewer, for evaluating the bisulfite sequencing results.

204 5-aza-2'-deoxycytodine treatment and bisulfite sequencing revealed hypermethylation of latexi

205 Bisulfite sequencing revealed no DNA-methylation in this

206 Bisulfite sequencing revealed that both human and mouse

207 Bisulfite sequencing revealed that cytosine-guanine dinu

208 Further Bisulfite sequencing revealed that miR-210 is embedded i

209 Bisulfite sequencing revealed that promoter CpG islands

210 Bisulfite sequencing revealed that the increased L1 expr

211 Time-series whole genome bisulfite sequencing reveals extensive gain of CHH methy

212 patterns through an integrative analysis of bisulfite-sequencing, RNA-sequencing, and siRNA-sequenci

213 Using both reduced representation bisulfite sequencing (RRBS) and microarray, we determine

214 g was performed using reduced representation bisulfite sequencing (RRBS) and RNA-sequencing (RNA-Seq)

215 tion (MethylCap-seq), reduced representation bisulfite sequencing (RRBS) and the Infinium HumanMethyl

216 e wide application of reduced representation bisulfite sequencing (RRBS) and whole genome bisulfite s

217 e apply our method to reduced representation bisulfite sequencing (RRBS) data from multiple regions o

218 Reduced representation bisulfite sequencing (RRBS) is a cost-effective approach

219 Reduced representation bisulfite sequencing (RRBS) is a powerful yet cost-effic

220 raditional MspI-based Reduced Representation Bisulfite Sequencing (RRBS) protocol to all restriction

221 g mutated HTT, we use reduced representation bisulfite sequencing (RRBS) to map sites of DNA methylat

222 We used reduced representation bisulfite sequencing (RRBS) to profile DNA methylation i

223 e previously reported reduced representation bisulfite sequencing (RRBS), a bisulfite-based protocol

224 hylC-seq, or BS-seq), reduced-representation bisulfite sequencing (RRBS), and enrichment-based method

225 thods MethylC-seq and reduced representation bisulfite sequencing (RRBS), and the enrichment-based te

226 lation differences by reduced representation bisulfite sequencing (RRBS), we determined that, over ti

227 uencing (RNA-Seq) and reduced-representation bisulfite sequencing (RRBS).

228 nes and tissues using reduced representation bisulfite sequencing (RRBS).

229 y using genomic-scale reduced representation bisulfite sequencing (RRBS).

230 We report a single-cell bisulfite sequencing (scBS-seq) method that can be used

231 me maps from single cells, using single-cell bisulfite sequencing (scBS-seq), allowing the quantitati

232 Bisulfite sequencing showed that DNA methylation was res

233 DNA gel-blot analysis and genomic bisulfite sequencing showed that silencing of the epiall

234 Targeted and whole-genome bisulfite sequencing showed that the induced ripening of

235 Bisulfite sequencing shows that CG dinucleotides in the

236 Whole-genome bisulfite sequencing shows these variable cDMRs are rela

237 n patterns using single-cell, locus-specific bisulfite sequencing (SLBS).

238 Here, we have analyzed multiple bisulfite sequencing studies to address the methylation

239 Whole-genome bisulfite sequencing suggested that STA1 and the RdDM pa

240 present a genome-wide approach, Tet-assisted bisulfite sequencing (TAB-Seq), that when combined with

241 ed bisulfite-sequencing method, Tet-assisted bisulfite sequencing (TAB-seq), which can identify 5-hmC

242 The rapid emergence of bisulfite-sequencing technologies enables performing suc

243 sign, particularly in reduced representation bisulfite sequencing, there is a need to develop more fl

244 We have now used whole-genome bisulfite sequencing to analyze the methylomes of Dnmt2-

245 lation27 Illumina platform, we performed DNA bisulfite sequencing to compare the methylation status i

246 s hypothesis, we used reduced representation bisulfite sequencing to examine the cross-sectional geno

247 Using whole genome bisulfite sequencing to examine uncharted regions of the

248 Here we applied oxidative bisulfite sequencing to generate whole-genome DNA methyl

249 we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulatio

250 microdissection with reduced representation bisulfite sequencing to identify cancer-associated DNA m

251 we employed Enhanced Reduced Representation Bisulfite Sequencing to interrogate the epigenome of the

252 By applying whole-genome bisulfite sequencing to maize, we found that transposons

253 hylcytosine and very recent modifications of bisulfite sequencing to resolve 5-hydroxymethylcytosine

254 A recent study used genome-wide bisulfite sequencing to survey differences in DNA methyl

255 We used replicated bisulfite-sequencing to investigate patterns of DNA meth

256 Currently available bisulfite sequencing tools frequently suffer from low ma

257 g (n = 12 per group), unbiased capture array bisulfite sequencing was combined with subsequent matrix

258 lation and hydroxymethylation with oxidative bisulfite sequencing was conducted and correlated with c

259 Whole-genome bisulfite sequencing was conducted to assess the dynamic

260 Targeted bisulfite sequencing was performed with a subset of plac

261 PCK1 promoter methylation analysis using bisulfite sequencing was significantly reduced in six ou

262 Reduced representational bisulfite sequencing was then used to compare the differ

263 Finally, oxidative bisulfite sequencing was used to differentiate methylati

264 Whole-genome bisulfite sequencing was used to produce nucleotide reso

265 By using methylation-specific PCR and bisulfite sequencing we demonstrate that miR-663 promote

266 Using whole genome bisulfite sequencing we identified hundreds of different

267 Using targeted bisulfite sequencing, we examined methylation of 2100 ge

268 By performing genome-scale bisulfite sequencing, we find that DNMT3B-deficient iPSC

269 Using bisulfite sequencing, we found that SLE T cells displaye

270 Here, using high-throughput bisulfite sequencing, we identified an APL-associated hy

271 Using bisulfite sequencing, we observed the 5'-untranslated re

272 Using whole-genome bisulfite sequencing, we show that crossover remodeling

273 Using restriction-sensitive PCR and bisulfite sequencing, we showed that AL2-mediated TGS su

274 Through whole-genome bisulfite sequencing, we showed that DNA methylation wen

275 equencing and high-coverage sequence-capture bisulfite sequencing were applied to mutant lines to det

276 RNA-Seq and multiplex bisulfite sequencing were performed to examine gene expr

277 RNA sequencing and reduced representation bisulfite sequencing were used to create transcriptomic

278 Whole-genome bisulfite sequencing (WGBS) allows genome-wide DNA methy

279 Whole-genome bisulfite sequencing (WGBS) analysis revealed that Tet-m

280 Here, we performed whole-genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-seq)

281 Using sample-matched whole-genome bisulfite sequencing (WGBS) as a gold standard, we demon

282 mples and compared results with whole-genome bisulfite sequencing (WGBS) data obtained for the same s

283 igenetic energy landscapes from whole-genome bisulfite sequencing (WGBS) data that enable us to quant

284 Whole-genome bisulfite sequencing (WGBS) has emerged as the gold-stan

285 Whole-genome bisulfite sequencing (WGBS) is the gold standard for stu

286 Techniques like whole-genome bisulfite sequencing (WGBS) make it possible to determin

287 y process in A. mellifera using whole genome bisulfite sequencing (WGBS) method.

288 ddress this issue, we performed whole-genome bisulfite sequencing (WGBS) of newborn and centenarian g

289 The cost of whole-genome bisulfite sequencing (WGBS) remains a bottleneck for man

290 Whole genome bisulfite sequencing (WGBS) revealed that integrin alpha

291 Recent developments in whole genome bisulfite sequencing (WGBS) technology have enabled geno

292 increased disease risk we used whole genome bisulfite sequencing (WGBS) to analyze changes in DNA me

293 methylated regions (DMRs) from whole-genome bisulfite sequencing (WGBS).

294 These include whole-genome bisulfite sequencing (WGBS, MethylC-seq, or BS-seq), red

295 e increases in methylation were validated by bisulfite sequencing, where they occurred in a minority

296 Here, we performed whole-genome bisulfite sequencing, which is a comprehensive and unbia

297 dresses statistical challenges introduced by bisulfite-sequencing while controlling for complex sourc

298 e used whole-genome sequencing, whole-genome bisulfite sequencing, whole transcriptome (RNA-seq) and

299 ned using single cell reduced representation bisulfite sequencing, with a 66-fold increase in the fra

300 rporation of the 5D4 DNA polymerase into the bisulfite sequencing workflow thus promises significant