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1 ers and low in the daughters of the anterior blastomere.
2 e, rafts were also apically enriched in each blastomere.
3 orm anterior pharyngeal muscles from the ABa blastomere.
4 d hypodermis, tissues normally made by the C blastomere.
5 ntrated in the late-dividing, two-cell-stage blastomere.
6 s a result of G2 cell cycle arrest of the P4 blastomere.
7 e) determines the developmental fate of each blastomere.
8 in misorientation of the spindle of the ABar blastomere.
9 mic fashion unique for each protein and each blastomere.
10 is seen with features of totipotent two-cell blastomeres.
11  distributed equally to somatic and germline blastomeres.
12 ely repressing transcription in all germline blastomeres.
13 ly in oocytes and segregated to all germline blastomeres.
14 pendent upon the unequal division of vegetal blastomeres.
15 es high PIE-1 levels in oocytes and germline blastomeres.
16       Similar results are seen with isolated blastomeres.
17 lar programs of the embryo and of individual blastomeres.
18 regulated by the unequal division of vegetal blastomeres.
19 throughout the presumptive endoderm and B7.5 blastomeres.
20 etric distribution of Cdx2 mRNA in polarized blastomeres.
21 at the blastula stage from the most marginal blastomeres.
22  become the retina by injection into Xenopus blastomeres.
23 scription in both somatic and later germline blastomeres.
24 t mature differently in somatic and germline blastomeres.
25 n that blocks mRNA transcription in germline blastomeres.
26 ad4 homo- and heteromers in isolated Xenopus blastomeres.
27 e structures within the cytoplasm of vegetal blastomeres.
28 erated in the apical membrane of early stage blastomeres.
29 human embryonic stem (hES) cells from single blastomeres.
30 tive pathway both in NSM cells and in animal blastomeres.
31  furrow, and remains enriched in animal pole blastomeres.
32 essential to set the identities of the early blastomeres.
33 ture, including the identity of the columnar blastomeres.
34 ng that hES cells can be derived from single blastomeres.
35 ributed in the clear apical cytoplasm of the blastomeres.
36 riched at the surface of all non-mesenchymal blastomeres.
37 mulation of its message in macromere-derived blastomeres.
38 lastomere and to mutant embryos with extra C blastomeres.
39  the apical aPKC in the polarisation of frog blastomeres.
40 es with GlsA, both in interphase and mitotic blastomeres.
41 activation of HIPPO signaling in the outside blastomeres.
42 y to suppress HIPPO signaling in the outside blastomeres.
43 olarity proteins to contact-free surfaces of blastomeres.
44 d, ANE regulatory state to the most anterior blastomeres.
45  MEX-3, enabling ZIF-1 expression in somatic blastomeres.
46 or proper nuclear division in large dividing blastomeres.
47 egans pharynx is produced from the embryonic blastomeres ABa and MS.
48 otes PtdIns(3,4,5)P(3) synthesis at sites of blastomere adhesion at all cleavage stages.
49                         To determine whether blastomere allocation to the two earliest lineages is ra
50  as expected, but surprisingly three somatic blastomeres also remain poor in H3K4me3.
51 indicate that in vivo, PDCD2 is critical for blastomere and ESC maintenance by contributing to the re
52 l inhibition of Xbves activity within the A1 blastomere and its derivatives completely randomizes mov
53             In this study, we first quantify blastomere and nuclear sizes in X. laevis embryos, demon
54 vision orientation of the endomesoderm (EMS) blastomere and the endoderm fate of the posterior EMS da
55 type embryos with mutant embryos lacking a C blastomere and to mutant embryos with extra C blastomere
56 s destabilized/degraded in animal hemisphere blastomeres and became localized to the nuclei of the fo
57 itosis during the rapid cleavage division of blastomeres and in somatic cells.
58           Canonical Wnt signaling in vegetal blastomeres and Nodal signaling in presumptive oral ecto
59         Characteristics of arrested prophase blastomeres and oocytes are the alignment of condensed c
60 pecific transcriptional program in the inner blastomeres and prevents segregation of the TE and ICM l
61  150 nonredundant protein groups between all blastomeres and replicate measurements, we found signifi
62 gion of the embryo but is inactive in animal blastomeres and show that the inability of LvDsh to func
63 ginine methyltransferase CARM1 in individual blastomeres and show that this directs their progeny to
64 data suggest heterogeneity among early-stage blastomeres and that the UCSFB lines have unique propert
65    Thirteen uniquely identifiable individual blastomeres and two double cell combination deletions we
66 an be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic
67 adherin, the major cell-adhesion molecule of blastomeres, and present evidence that this modification
68 yos, nos-2 translation is repressed in early blastomeres, and this inhibition depends on a second reg
69 e exchange of cytoplasm and membrane between blastomeres; and (b) they are active in caveolar endocyt
70  the MOEP19 domain was re-established at the blastomere apices.
71 e and fragmentation parameters of individual blastomeres are diagnostic of ploidy, amenable to automa
72                      In C. elegans, germline blastomeres are initially kept transcriptionally quiesce
73                                              Blastomeres are removed from morula (eight-cell)-stage e
74      However, when such individual four-cell blastomeres are surrounded by blastomeres from random po
75                               Thus, the B7.5 blastomeres are the only cells to express sustained leve
76  found in two-cell (2C) embryos in which the blastomeres are totipotent.
77  When CD divides, the larger D and smaller C blastomeres arise invariantly on the left and right side
78           Three-dimensional visualization of blastomere arrangement and diagenetic cement in cleavage
79 f SoxB1 in the progeny of different tiers of blastomeres arrayed along the animal-vegetal axis.
80 , H3K4me3 deposition is poor in the germline blastomere, as expected, but surprisingly three somatic
81               The unequal division of the CD blastomere at second cleavage is critical in establishin
82 on of maternal proteins to the left or right blastomere at the first cell division.
83 d annelids), it has long been known that one blastomere at the four-cell stage, the D cell, and its d
84 d that GlsA is about equally abundant in all blastomeres at all cleavage stages examined but that Hsp
85 m specification: appropriate polarisation of blastomeres at the 8- and 16-cell stage and then the mai
86 n of different histone modifications between blastomeres at the morula stage and cell sub-populations
87 sed the experimental potential of individual blastomeres based on their level of Cdx2-eGFP expression
88 human embryos to address the hypothesis that blastomere behaviour may reflect ploidy during the first
89 ation further elevated overall blastomere-to-blastomere biases during the two- to 16-cell embryo stag
90 , we revealed that the initial blastomere-to-blastomere biases emerge as early as the first embryonic
91                                       Single-blastomere-biopsied embryos developed to term without a
92 rs, showed ever-increasing asymmetry between blastomeres (bistable pattern), supposedly controlled by
93 nd sufficient for cas expression in marginal blastomeres, Bon and Gata5 are unable to induce cas in a
94                            The C. elegans MS blastomere, born at the 7-cell stage of embryogenesis, g
95 is switch occurs asynchronously in different blastomeres but is independent of clonal cell heritage a
96 epresses transcription in the later germline blastomeres but not in the earlier germline blastomeres
97 in was found at the apex of outer, polarized blastomeres but was undetectable in blastomeres of the i
98 o block serine 2 phosphorylation in germline blastomeres, but unexpectedly is not essential for trans
99        nos-2 RNA is also degraded in somatic blastomeres by a process that is independent of translat
100 canonical Wnt pathway to the dorsal marginal blastomeres by defining the domain where the Wnt8a activ
101 omes promotes endoderm induction in marginal blastomeres by facilitating the assembly of a transcript
102  ZIF-1 continues to be repressed in germline blastomeres by POS-1, a germline blastomere-specific pro
103 ttern of symmetric/asymmetric divisions of a blastomere can be influenced by its origin in relation t
104  also show that the genetic lineage of early blastomeres can be traced by DNA methylation analysis.
105 g CTNNB1 undergo fission and these separated blastomeres can become small trophoblastic vesicles, whi
106 f all species, yet how neighboring embryonic blastomeres can contribute to different germ layers has
107 ndogenous VegT and/or Vg1 in ventral vegetal blastomeres can induce a neural fate, but only allows ex
108 ntly, injection of Eg5 into TPX2-CT-arrested blastomeres causes resumption of cleavage.
109                   Addressing the question of blastomere cell fate may be of practical importance, bec
110 n from blastocyst inner cell mass and single blastomere cells without a need to destroy the embryo.
111 nic trophectoderm are established when eight blastomeres compact to form polarized morulae in preimpl
112 m human embryos at the blastomere, polyploid blastomere, compaction, morula and blastocyst-like stage
113 e remarkable genomic heterogeneity among the blastomeres comprising a single embryo during human prei
114 opy number of parental genomes in 116 single blastomeres comprising entire preimplantation bovine emb
115  opposing lineage specifiers within an early blastomere constantly compete with each other based on t
116 een blastomere progeny so that the loss of a blastomere could be compensated for during development.
117 hen the mass of yolk-free proteins in single blastomeres decreased from approximately 0.8 mug (16-cel
118 ge is not exactly symmetric, the content per blastomere decreases roughly by half with each cell divi
119                           On the other hand, blastomere deletion experiments indicate that the D quad
120 ding proteins, with expression in a specific blastomere dependent upon the precise combination of the
121                            Expression in the blastomeres depends upon a conserved Nodal response elem
122                                        These blastomere-derived cell aggregates are plated into micro
123                                The resulting blastomere-derived outgrowths are cultured in the same m
124 e-cell gene expression analysis reveals that blastomeres develop cell autonomously, with some cells a
125 altered subcellular localization of TEAD4 in blastomeres dictates first mammalian cell fate specifica
126 tomeres, we report highly reproducible inter-blastomere differences among 10 2-cell and five 4-cell m
127                                        Inter-blastomere differences created coexpression gene network
128 se data substantiate the hypothesis of inter-blastomere differences in 2- and 4-cell mouse embryos, a
129 t progeny of mesoderm and ectoderm producing blastomeres display intra-germ layer compensation.
130 rically dividing embryos may determine which blastomeres divide asymmetrically and which do not.
131                        It requires that some blastomeres divide asymmetrically to direct cells to the
132 rtebrate development, apicobasally polarised blastomeres divide to produce inner non-polarised cells
133 such asymmetric division occurs when the EMS blastomere divides to produce MS, a mesoderm precursor,
134 D-modeling framework that iteratively infers blastomere division positions and orientations, and cons
135 tribute cells to the retina; ventral vegetal blastomeres do not form retina even when provided with n
136 ntain long-term contacts between nonadjacent blastomeres during expansion of the interstitial space i
137 s than in posterior (symmetrically dividing) blastomeres during the period of asymmetric division.
138 modification expression was detected between blastomeres earlier in human embryos at the four- to eig
139 arge cells following zygote formation, early blastomeres employ modified cell divisions.
140               In C. elegans the 4-cell stage blastomere EMS is an endomesodermal precursor.
141                            Isolated cleavage blastomeres exhibit polarized actin-dependent fluid phas
142 f either factor in dorsal-animal retinogenic blastomeres expands expression of neural/retinal genes a
143 n response to stabilized betacatenin, dorsal blastomeres express the closely related transcriptional
144 olk syncitial layer was normal, the marginal blastomeres failed to migrate toward the vegetal pole an
145                             By transplanting blastomeres from a wild-type (WT) zebrafish into a lakri
146 ontrast, chimaeras made from four-cell stage blastomeres from early meridional divisions develop norm
147 dual four-cell blastomeres are surrounded by blastomeres from random positions, they are able to cont
148 tation genetic diagnosis requires removal of blastomeres from the early human embryo.
149 cal and growth-rate heterogeneity, and mouse blastomeres from the same embryo have stochastic differe
150  Controversy exists as to whether individual blastomeres from two-cell-stage mouse embryos have ident
151                                        Inter-blastomere gene expression differences dominated between
152 pindle into the apicobasal axis of polarised blastomeres generates inner and outer cells with differe
153 d how in the majority of embryos, individual blastomeres give rise to distinct blastocyst regions.
154 ell division failure in early Xenopus embryo blastomeres has been attributed to a role of maskin in r
155 er, the relationship between PGCCs and giant blastomeres has never been studied.
156 s of DNA synthesis and mitosis, and arrested blastomeres have abnormal spindles, clustered centrosome
157 stomeres, we observed that the blastomere-to-blastomere heterogeneity in 8-, 16-, 32-, and 50-cell em
158 otch signal that specifies embryonic ABa/ABp blastomere identities at the four-cell stage.
159  diversification of anterior-posterior (A-P) blastomere identities.
160 gh we have identified isolated examples of a blastomere imparting a statistically significant bias, w
161 3K4me3 are descendants of the first germline blastomere, implying an activity that impedes on H3K4me3
162 ated in oocytes and localize to one or a few blastomeres in a spatially and temporally dynamic fashio
163            Here we investigate how embryonic blastomeres in C. elegans develop into foregut (pharynx)
164 NPP-16 and CDK-1 function to arrest prophase blastomeres in C. elegans embryos.
165 maging with karyotypic reconstruction of all blastomeres in four-cell human embryos to address the hy
166 hat the orientation of division of polarised blastomeres in the 8- and 16-cell stage embryo determine
167 endoderm (ME) cells are the two most vegetal blastomeres in the early developing embryo of the marine
168              Only a subset of cleavage stage blastomeres in the Xenopus embryo is competent to contri
169                         Removal of any other blastomeres, including PNM progenitors, did not interfer
170 ation dynamics specifically in the posterior blastomere, independently of regulators previously impli
171 f the dominant-negative mRNA into individual blastomeres indicated that the effect was exerted on the
172 dle polarization during division of the ABar blastomere, indicating that these cell surface proteins
173                               Using targeted blastomere injection, morpholino-based loss of function
174 fined to half of the embryo via 2-cell stage blastomere injections, the latter does not produce the o
175 noise, and were sufficient to cluster sister blastomeres into distinct groups.
176 ar whether pathways governing segregation of blastomeres into the PSE lineage are conserved.
177 ther study it resulted in differentiation of blastomeres into trophoblast giant cells (TGCs), suggest
178  known if this lineage pattern means a given blastomere is committed to its specific fate, indicative
179 t of clonal cell heritage and of whether the blastomere is within the inner cell mass or the trophoec
180  we performed bottom-up proteomics on single blastomeres isolated by microdissection from 2-, 4-, 8-,
181 0 protein groups were quantified across four blastomeres isolated from a 16-cell embryo.
182 prehensive quantitative proteomics on single blastomeres isolated from these early stage embryos can
183                We propose that single cells (blastomeres) isolated from early stage invertebrate, amp
184 hen taken together, our results suggest that blastomere lineage does not impart a widespread bias for
185                 We propose that as embryonic blastomeres lose their developmental plasticity, hermaph
186 s, human zygotes and perhaps human embryonic blastomeres may be useful supplements to human oocytes f
187 s able to influence positional allocation of blastomeres, mediating preferential localization to the
188 rmined until the time of formation of the 4d blastomere (mesentoblast).
189 he extent of asymmetry was minimized between blastomeres (monostable pattern), whereas other genes, i
190                             The 8-cell stage blastomere MS is primarily a mesodermal precursor, givin
191 ic to the ectopic spindle rotation in the AB blastomere of par-3 mutant embryos.
192 the late-dividing but not the first-dividing blastomere of two-cell embryos and, by lineage tracing a
193 ricted to a cortical cytoplasmic crescent in blastomeres of 2-, 4- and 8-cell embryos.
194                                         When blastomeres of 4- to 8-cell stages were dissociated, the
195 tenin revealed differential expression among blastomeres of 8- to 10-cell human embryos.
196 ell lines (designated UCSFB1-10) from single blastomeres of four 8-cell embryos and one 12-cell embry
197                          In rapidly dividing blastomeres of medaka (Oryzias latipes) and in somatic c
198 are fertile even when micromeres, the parent blastomeres of small micromeres, are deleted.
199 ing highly heterogeneously expressed between blastomeres of the 4-cell embryo, with Sox21 showing one
200  fusion proteins are overexpressed in single blastomeres of the 4-cell stage embryo, the progeny of t
201     By ectopically expressing hlh-1 in early blastomeres of the C. elegans embryo, we show that CeMyo
202 We found that Vasa protein is present in all blastomeres of the early embryo and that its abundance o
203                                              Blastomeres of the early mouse embryo are thought to be
204  of a differentiated oocyte into pluripotent blastomeres of the embryo.
205 olarized blastomeres but was undetectable in blastomeres of the inner cell mass.
206 ymmetry is reversible upon disaggregation of blastomeres of the two- and four-cell embryo.
207 ion of TPX2 or its C terminus (TPX2-CT) into blastomeres of two-cell embryos led to potent cleavage a
208 lso causes CSF arrest in egg extracts and in blastomeres of two-cell embryos.
209 tation techniques to investigate whether the blastomeres of two-cell-stage mouse embryos can reprogra
210  express Pax7, but overexpression of Pax7 in blastomeres of whole embryos that populate the myogenic
211 nerates Ca(2+) transients in the superficial blastomeres of zebrafish blastulae when the nuclear accu
212 onstrate that ZO-1 siRNA delivery inside the blastomeres of zona-weakened embryos using electroporati
213  inclined 'lineage strength' that pushes the blastomere onto a predisposed, yet flexible, lineage tra
214 normal-appearing larvae if the prospective D blastomere or 3D macromere is removed.
215  expressed at least two isoforms in the same blastomere or oocyte, which unambiguously demonstrated t
216 ount either the spatial origin of individual blastomeres or the spatial allocation and fate of their
217                        By growing the single blastomere overnight, the resulting cells could be used
218                                  In germline blastomeres, P-bodies are maintained as core granules la
219                                   In somatic blastomeres, P-bodies recruit the decapping activators L
220  blastomeres but not in the earlier germline blastomeres P0 and P1.
221 ucing a transcriptionally repressed germline blastomere (P1-P4).
222 enerate transcriptionally repressed germline blastomeres (P1-P4) and somatic sisters that become tran
223         We found that the germline precursor blastomere, P4 , fails cytokinesis, leaving a stable cyt
224 angle of division, such as the position of a blastomere, play a major role in the specification of TE
225  indistinguishable from human embryos at the blastomere, polyploid blastomere, compaction, morula and
226             Interestingly, the three somatic blastomeres poor in H3K4me3 are descendants of the first
227 st mouse embryos the progeny of one two-cell blastomere primarily populate the embryonic part of the
228 ion and premature division in early germline blastomeres, processes that are independent of PIE-1 fun
229                                       The MS blastomere produces one-third of the body wall muscles (
230 elopment, or if regulation can occur between blastomere progeny so that the loss of a blastomere coul
231         Increasing Cdx2 levels in individual blastomeres promotes symmetric divisions, thereby alloca
232         Organizer formation occurs in dorsal blastomeres receiving both maternal Wnt and zygotic Noda
233 ME cells and PNM cells in gastrulation using blastomere recombinations and confocal microscopy.
234 ryos, transcriptional repression in germline blastomeres requires PIE-1 protein.
235 tion, while overnight culture of dissociated blastomeres resulted in formation of re-aggregated embry
236                         We find that loss of blastomeres results in compensation.
237               These results demonstrate that blastomeres retain reprogramming activities and support
238 lating early embryonic fate specification by blastomere separations, exposure to lithium, and dominan
239 ing from the major ectodermal progenitor (AB blastomere) several cell divisions later, thereby preven
240 ngth-dependent microtubule forces that probe blastomere shape and yolk gradients, biased by cortical
241 y of these equatorially or obliquely derived blastomeres show developmental abnormalities in both lat
242                        All the other somatic blastomeres show robust deposition of H3K4me3.
243                Time-lapse imaging of labeled blastomeres shows that the animal cap tissue moves into
244                                     Germline blastomere-specific localization of PIE-1 depends, in pa
245 in germline blastomeres by POS-1, a germline blastomere-specific protein.
246 o embryonic genome activation, and others to blastomere-specific RNA depletion.
247 gle-cell transcriptome reveal enrichment for blastomere-specific signature and a dynamic DNA methylom
248  failed mitosis/cytokinesis is common in the blastomere stage of the preimplantation embryo.
249 no oligonucleotides (MOs) microinjected into blastomeres suppressed hnRNP K expression from neural pl
250 tment, the progeny of the resulting two-cell blastomeres tend to populate the respective embryonic an
251 undant in anterior (asymmetrically dividing) blastomeres than in posterior (symmetrically dividing) b
252 a asymmetric divisions of eight- and 16-cell blastomeres that allocate cells to inner and outer posit
253                       Divisions of polarised blastomeres that allocate polar cells to outer and apola
254  equatorial or oblique allows us to identify blastomeres that differ in their fate and in their devel
255  homeobox factor PAL-1 can activate hlh-1 in blastomeres that either lack POP-1/TCF or that have down
256           These lobes normally fuse with the blastomeres that give rise to the D quadrant at the two-
257     Altered spindle orientations occurred in blastomeres that had direct contact with one of the ME c
258 ects of chimaeras made from the most vegetal blastomeres that result from later second cleavages are
259 <0.2% of the total protein content in single blastomeres that were isolated from the 16-cell frog (Xe
260 c arginine residues are maximal in four-cell blastomeres that will contribute to the inner cell mass
261 bryo is composed of superficially equivalent blastomeres that will generate both the embryonic inner
262       We also find that, as in early somatic blastomeres, the degradation of PIE-1 in Z2/Z3 is facili
263 ) is detected in wild-type-arrested prophase blastomeres, the inactive state is not detected in the a
264  that PGCCs represent somatic equivalents of blastomeres, the most primitive cancer stem cells report
265 TE-specific transcriptional program in inner blastomeres, thereby allowing their maturation toward th
266 In C. elegans, Wnt signaling specifies the E blastomere to become the endoderm precursor.
267 s unsettled whether the contribution of each blastomere to these two lineages can be accounted for by
268 levels of H3 arginine methylation predispose blastomeres to contribute to the pluripotent cells of th
269 , to attenuate Nodal responsiveness and bias blastomeres to ectoderm and mesoderm fates.
270 ess the ability of dorsal-animal retinogenic blastomeres to form retina, converting the lineage from
271 in the vegetal region repress the ability of blastomeres to form retina.
272 es, might modulate the response of embryonic blastomeres to growth factors and other signals that gov
273 NC-120/SRF and HND-1/HAND, can convert naive blastomeres to muscle when overproduced ectopically in t
274 ion of fluid and differentiation of nonpolar blastomeres to polar trophoblast cells.
275 ighlighted by the ability of separated early blastomeres to produce a whole organism.
276 d on the intrinsic ability of the individual blastomeres to respond to signals directing epiboly and
277         We propose that restriction of early blastomeres to the pharyngeal fate depends on both repre
278     To investigate the contribution of early blastomeres to the veliger larva, we used intracellular
279 iptional activation further elevated overall blastomere-to-blastomere biases during the two- to 16-ce
280 tation embryos, we revealed that the initial blastomere-to-blastomere biases emerge as early as the f
281  different blastomeres, we observed that the blastomere-to-blastomere heterogeneity in 8-, 16-, 32-,
282                    Here, by analyzing single-blastomere transcriptome data from mouse and human pre-i
283                         Experiments in which blastomere transplantation is combined with foxa MASO tr
284        Clonal loss-of-function, inflicted by blastomere transplantation or gene-transfer assays, high
285                                      Through blastomere transplantation, we find that tal1 plays a ce
286 iptome analysis in a single mouse oocyte and blastomere using a deep-sequencing approach.
287 asynchronous cell division, with diminishing blastomere volume as cleavage proceeded.
288 vated [Ca2+]i characteristic of the marginal blastomeres was suppressed.
289 however, by creating embryos with over-sized blastomeres we present evidence of a developmental progr
290  our mRNA-Seq assay with only a single mouse blastomere, we detected the expression of 75% (5,270) mo
291 aring the protein expression among different blastomeres, we observed that the blastomere-to-blastome
292 g deep single-cell RNA-seq of matched sister blastomeres, we report highly reproducible inter-blastom
293         When chromosomes from one of the two blastomeres were replaced with the chromosomes of an emb
294 l lineage specification of MS and its sister blastomere, whereas the inductive interaction promotes t
295 ed reproducible bimodal expression in sister blastomeres, which cannot be explained by random fluctua
296 are required for Mesp expression in the B7.5 blastomeres, which constitute the heart field.
297  single pair of endomesoderm cells, the A6.3 blastomeres, which form part of the anterior endoderm, h
298 em (TS) cell lines were produced from single blastomeres, which maintained normal karyotype and marke
299 gl2 localises to the basolateral membrane of blastomeres, while Crumbs3 localises to the apical and b
300 ntirely from a single progenitor cell, the E blastomere, whose identity is specified by GATA type tra

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