ライフサイエンスコーパス: blastula

1 attern driving morphogenesis of the ascidian blastula.

2 ed mesoderm cells at the vegetal pole of the blastula.

3 formation of a blastopore, an opening in the blastula.

4 g pathways control the actin skeleton in the blastula.

5 toderm and the blastoderm margin at the late blastula.

6 is required for wound healing in the Xenopus blastula.

7 zer region in the dorsal side of the Xenopus blastula.

8 mRNAs enriched in microsomes in the Xenopus blastula.

9 ucing tbx16/spt and beta catenin mRNA in the blastula.

10 rinuclear sites in the vegetal region of the blastula.

11 d and dramatic loss of tbx16/spt mRNA in the blastula.

12 atial determinant of expression in the early blastula.

13 ression of SpHE in the very early sea urchin blastula.

14 area rather than the equatorial zone of the blastula.

15 gene controls the patterning of the Xenopus blastula.

16 e animal hemisphere from oocyte through late blastula.

17 a developmental stage resembling mesenchyme blastula.

18 ts the co-repressor Tle/Groucho in the early blastula.

19 y promoting Ca(2+) transients throughout the blastula.

20 nuclear translocation in dorsal cells of the blastula.

21 cells into ocular-fated regions of wild-type blastulas.

22 -P) oriented cell divisions, starting at the blastula 64-cell stage.

23 Here we used conjugated and aged blastula AC to avoid diffusion of endogenous molecules f

24 rly cleavage pattern and the fate map of the blastula, along with similarities in larval morphology.

25 receptor expression on the left side of the blastula also elicits heart reversals and altered nodal

26 in the apical plate ectoderm of the hatching blastula and are confined to the apical organ at least t

27 l vegetal pole mesoderm of the late sea star blastula and compare them to the GRN for the same embryo

28 e larva begins to be set up between the late blastula and early gastrula stage.

29 At late mesenchyme blastula and early gastrula stages, greater than 90% of

30 d by macromere descendants during mesenchyme blastula and early gastrula stages.

31 ours prior to gastrulation, between the late blastula and early mesenchyme blastula stages.

32 polarity and to define signaling centers in blastula and gastrula stage embryos.

33 f ARHGEF11 that is expressed ubiquitously at blastula and gastrula stages and is enriched in neural t

34 Early asymmetric cell divisions at blastula and gastrula stages give rise to the superficia

35 tion of the progeny was followed through the blastula and gastrula stages in order to determine their

36 ical cell death, while cell death during the blastula and gastrula stages is random and predominantly

37 During blastula and gastrula stages of Xenopus development, cel

38 family of proteins are expressed during late blastula and gastrula stages of Xenopus development.

39 tal endomesoderm expresses lvnumb during the blastula and gastrula stages, and that the protein is lo

40 Xenopus ALDH1 was not expressed at blastula and gastrula stages, but was expressed at the n

41 hich are products of an FGF induction at the blastula and gastrula stages, initially express neural p

42 During the blastula and gastrula stages, mutant cells are more cohe

43 d maternally and in mesoderm and ectoderm at blastula and gastrula stages.

44 Pk9.7 is transcribed only during blastula and gastrula stages.

45 these signalling molecules are found in the blastula and gastrula vegetal pole and induce both endod

46 , are expressed early in development, in the blastula and gastrula, but not at later stages when a pu

47 l-resolution fate maps of the zebrafish late blastula and gastrula, we demonstrate that individual ce

48 cation of signaling centers that pattern the blastula and gastrula.

49 rive transgenic endodermal expression in the blastula and gastrula.

50 APD regulatory genes expressed in the early blastula and have shown that expression of most of them

51 gene is expressed in the endomesoderm in the blastula and later the gut of the embryo, and is require

52 which arises in part from the centre of the blastula and localises to the deep endoderm adjacent to

53 odermal MAPK activity increased between late blastula and midgastrula stages.

54 its anterior-posterior axis during the late blastula and subsequent stages involved a posterior sign

55 buted across the vegetal half of the Xenopus blastula and that this activity contributes to mesoderm

56 senchyme cells of the approximately 200-cell blastula and, beginning at about the same time, accumula

57 derive from the marginal region of the late blastula, and cells located nearer the animal pole form

58 rsal yolk syncytial layer (YSL) in the early blastula, and is independent of Nodal signaling.

59 to the future endoderm and mesoderm by late blastula, and to the posterior mesoderm by mid-gastrula.

60 the expression patterns of these markers in blastula- and gastrula-stage chick embryos, using whole-

61 tivated cell sorting from disaggregated late blastula- and gastrula-stage sea urchin embryos accordin

62 est regulatory factors are also expressed in blastula animal pole cells and promote pluripotency in b

63 in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo.

64 We have used the Xenopus blastula as a model system to study this problem.

65 that the gene is first activated in the 20-h blastula, but there remain only about 100 molecules of b

66 Xnr expression at blastula can be activated by the vegetal determinants Ve

67 We conclude that isolated blastula cells can sense and respond individually to act

68 Using dissociated blastula cells of Xenopus exposed to activin for only 10

69 in rounded, yet polarised, isolated Xenopus blastula cells orient randomly, but align into an experi

70 have evolved as a consequence of a subset of blastula cells retaining activity of the regulatory netw

71 Xenopus blastula cells show a morphogen-like response to activin

72 Here we use zebrafish blastula cells that are responsive Wnt signaling to demo

73 nguish between these ideas, we have cultured blastula cells under conditions which provide different

74 underpinnings of potency in neural crest and blastula cells.

75 age, and as a marker for single transplanted blastula cells.

76 s in several spindle defects and compromised blastula development.

77 s early as the one-cell stage and during the blastula divisions, it is preferentially inherited by su

78 ndergoes sequential cell divisions to form a blastula, each cell develops a network of cortical actin

79 be achieved by dissociating the cells of the blastula ectoderm.

80 receptor expression on the right side of the blastula elicits heart reversals and altered nodal expre

81 pressed initially in the animal pole of late blastula embryo and subsequently restricted to the ventr

82 dmrt1 is expressed early in the blastula embryo in a small group of presumptive ectoderm

83 n of the germ layers, the subdivision of the blastula embryo into endoderm, mesoderm and ectoderm.

84 However, in the mesenchyme blastula embryo LvNotch shifts strikingly in subcellular

85 We show that in the early blastula embryo, LvNotch is absent from the vegetal pole

86 In blastula embryo, the only MMP gene expressed was found t

87 pairs the induction of gastrulation genes in blastula embryos and results in failure of differentiati

88 ells transplanted to the margin of wild-type blastula embryos initially internalize with their neighb

89 e holozoans, green algae akin to Volvox, and blastula embryos of early metazoans or bilaterian animal

90 ously interpreted as animal resting eggs and blastula embryos, represent Thiomargarita-like sulphide-

91 Using explants of animal pole tissue from blastula embryos, which will differentiate into mesoderm

92 s into the floor of the blastocoele from the blastula epithelium and later become the skeletogenic me

93 throughout the entire region of the Xenopus blastula fated to become endoderm, and is important in c

94 of animal embryos, superficial cells of the blastula form a distinct lineage and adopt an epithelial

95 Furthermore, we show that early blastula formation was disrupted by pharmacological bloc

96 ng both dorsal and ventral cells of the late blastula from expressing dorsal genes.

97 PTU is teratogenic during late blastula, gastrulation, and neurulation; whereas MMI is

98 e show that the AE is specified by the early blastula in the absence of mesodermal signals but that c

99 After induction of the germ layers, the blastula is transformed by gastrulation movements into a

100 expression of cas, which begins in the late blastula, is regulated by Nodal signaling.

101 developmental stages of previously published blastula-like fossils, and they show evidence for cell d

102 pfoxc, which is first expressed in the early blastula only in the four small micromeres, and later on

103 ressed zebrafish Ccr7 or Ccl19.1 expands the blastula organizer and the dorsoanterior tissues at the

104 by the establishment of the dorsal Nieuwkoop blastula organizer, marked by the nuclear accumulation o

105 and that their expression begins during the blastula period with a zebrafish-specific family of miRN

106 e window for Vox function is during the late blastula period.

107 Finally, extension of this approach to the blastula preorganizer signaling center identified the tr

108 egion of the vegetal plate of the mesenchyme blastula primarily gave rise to homogeneous clones consi

109 Blastula protease 10 (BP10) is a metalloenzyme involved

110 opy data collection from a single sea urchin blastula required less than 2 min, thereby allowing gene

111 alters the fates of different regions of the blastula so that animal cell fate is changed from epider

112 ng domain of SpAN mRNA accumulation at early blastula stage ( approximately 150 cells).

113 act embryos, it can only do so prior to late blastula stage (stage 9), well before the onset of gastr

114 s broadly in the oral ectoderm at mesenchyme blastula stage and at later embryonic stages is refined

115 inning in the micromere lineage of the early blastula stage and continuing after gastrulation during

116 ol of gcm expression by the early mesenchyme blastula stage and maintains it through pigment cell dif

117 d Sp-SERCA mRNA begin at the 18 hour hatched blastula stage and peak 4-5-fold higher by 25 h at the m

118 tion of LvTbx2/3 initiated at the mesenchyme blastula stage and protein was present into the pluteus

119 creases dramatically in embryos at the early blastula stage and remains at a constant level throughou

120 iched in the vegetal plate of the mesenchyme blastula stage and Sp-vasa, Sp-nanos2, Sp-seawi, and Sp-

121 me1 and H3K27ac, are established as early as blastula stage and that Smad2/3 only strongly associates

122 2/3 mRNA begins in micromeres at the hatched blastula stage and then is lost from micromeres at the m

123 ce the otic marker Pax8 when recombined with blastula stage animal caps.

124 The arrest in interphase caused by treating blastula stage animals caps with aphidicolin can be reve

125 nt on FoxN2/3, but only until the mesenchyme blastula stage as foxN2/3 mRNA disappears from PMCs at t

126 Second, sqt mRNA is expressed in blastula stage boz mutants, indicating that boz is not e

127 Tfap2a in embryos blocked for Bmp from late blastula stage can restore development of both PPE and N

128 a dramatic inhibition of ciliogenesis at the blastula stage characterized by the assembly of short, p

129 alization of activated MAPK occurs in morula/blastula stage embryo animal and marginal zones coincidi

130 ocalized to the prospective oral side of the blastula stage embryo, a process that requires lefty.

131 of 3.0 kilobase pairs was undetected in the blastula stage embryo, induced in gastrula embryo, expre

132 NE is restricted to the anterior of the late blastula stage embryo, where it forms a simple neural te

133 red for opl regulation, we removed from late blastula stage embryos either the presumptive prechordal

134 phosphorylation of membrane proteins of the blastula stage embryos, but EGF4 stimulates phosphorylat

135 s in animal cap explants from Xenopus laevis blastula stage embryos.

136 genes that are repressed by maternal rest in blastula stage embryos.

137 Apoptosis remains infrequent during the late blastula stage followed by a gradual increase in frequen

138 xI1e (also known as Xema) is required at the blastula stage for normal formation of both the central

139 In zebrafish, the endoderm originates at the blastula stage from the most marginal blastomeres.

140 ally, and ndr1 and ndr2 are expressed during blastula stage in the blastoderm margin.

141 LvBrac initially appears at mesenchyme blastula stage in two distinct regions with embryonic ex

142 he up-regulation of CyIIIa that occurs after blastula stage is a function of zygotically transcribed

143 nopus, mesoderm induction by endoderm at the blastula stage is well documented, but the molecular nat

144 hat single cells in the vegetal plate of the blastula stage Lytechinus variegatus embryo could be lab

145 ease in concentration dramatically after the blastula stage of development, suggesting that the up-re

146 transcription of the late H1 gene at the mid-blastula stage of development.

147 gylocentrotus purpuratus is expressed at the blastula stage of embryogenesis throughout the vegetal p

148 At the mid-blastula stage of embryogenesis, temporally correlated w

149 e in a temporally specific manner at the mid-blastula stage of embryogenesis.

150 on of the sea urchin late H1 gene at the mid-blastula stage of embryogenesis.

151 The Xlim-1 gene is activated in the late blastula stage of Xenopus embryogenesis in the mesoderm,

152 A localizes to gcm+cells from the mesenchyme blastula stage onwards.

153 ranscripts began to accumulate at mesenchyme blastula stage primarily in ectoderm and then later were

154 on, and conversely, boz mRNA is expressed in blastula stage sqt mutants.

155 We then used blastula stage transplantation experiments to demonstrat

156 tant of the FGF receptor (XFD) after the mid-blastula stage uncouples mesoderm induction, which is no

157 ase during zebrafish development through the blastula stage was investigated through the use of domin

158 This transition occurs well after the blastula stage when the basal transcription machinery ca

159 xpressed by micromere descendants during the blastula stage, a time when signaling has been shown to

160 ty between the end of oogenesis and the late blastula stage, and at least a further 20-fold reduction

161 al mesendodermal gene expression at the late blastula stage, and fate mapping and gene expression stu

162 such as noggin, follistatin, and Xnr3-at the blastula stage, and requires beta-Catenin signaling.

163 Strong zygotic expression begins during the blastula stage, and the transcript is present at moderat

164 y genes expressed in this lineage up to late blastula stage, as identified in a genomewide survey.

165 probably skeletogenic territories during the blastula stage, as shown by in situ hybridization analys

166 cts using the tbr driver, which is active in blastula stage, block ingression.

167 Soon after the hatching blastula stage, BMP2/4 transcripts can be detected in pr

168 After the blastula stage, cyclin E mRNA and protein levels are ver

169 velopment, but, for a short interval at late blastula stage, is asymmetrically inactivated in future

170 ear SpSoxB1 gradually expands so that, after blastula stage, only cells in differentiating ectoderm a

171 n when the fish had been light deprived from blastula stage, ruling out a "trial and error" period of

172 es the loss of intercellular adhesion at the blastula stage, similar to that reported previously for

173 the presumptive SMCs and endoderm during the blastula stage, the time at which these two cell types a

174 ples of total RNA from embryos up to the mid-blastula stage, when zygotic transcription begins.

175 S-null (NS(-/-)) mice was aborted before the blastula stage.

176 t the mesoderm/endoderm boundary at the late blastula stage.

177 n immobilized in rosettes until the hatching blastula stage.

178 ion of beta catenin in ventral nuclei at the blastula stage.

179 -related signals released by endoderm at the blastula stage.

180 nset of zygotic transcription, after the mid-blastula stage.

181 F2 is essential for development past the mid-blastula stage.

182 ntrotus purpuratus embryos at the mesenchyme blastula stage.

183 levels of retinoic acid detection during the blastula stage.

184 ation in the vegetal plate at the mesenchyme-blastula stage.

185 earing in embryonic nuclei shortly after the blastula stage.

186 increased beta-catenin levels before the mid-blastula stage.

187 A in the egg and are not detected beyond the blastula stage.

188 s major control of CyIIIa function after the blastula stage.

189 nes occurs sequentially, spanning the entire blastula stage.

190 vates early steps for both fates during late blastula stage.

191 en is lost from micromeres at the mesenchyme blastula stage.

192 gment cells and their precursors starting at blastula stage.

193 nd localized to prospective oral ectoderm at blastula stage.

194 nisms which determine this process up to mid-blastula stage.

195 ipts are present throughout all cells at the blastula stage.

196 agments necessary for development beyond the blastula stage.

197 dy competent to receive a BMP4 signal at the blastula stage.

198 o pattern mesendoderm differentiation at the blastula stage.

199 ting to veg(1) derivatives by the mesenchyme blastula stage; (2) Spgcm, an orthologue of the fruit fl

200 lates in embryos beginning at the mesenchyme blastula stage; a RNA gel blot and in situ hybridization

201 enchyme (mesodermal) domain at late-cleavage blastula stage; and (3) Spfoxc, which is first expressed

202 NA is localized to the vegetal region of the blastula-stage embryo.

203 roximately 36, 000 founder fish by injecting blastula-stage embryos with one of two pseudotyped retro

204 e mRNA evenly distributed among the cells of blastula-stage embryos.

205 ations, other results show that in explanted blastula-stage marginal zones a distinct pattern develop

206 arget sites for specific proteins present in blastula-stage nuclear extract.

207 binding experiments reveal that a protein in blastula-stage nuclei interacts specifically with the my

208 he initial veg(2) endomesodermal domain; the blastula-stage separation of the central veg(2) mesoderm

209 This virus was injected into blastula-stage zebrafish, and 16% of the injected embryo

210 Furthermore, SSAP can undergo blastula-stage-specific homodimerization through its GQ-

211 n the generation of endodermal cells at late blastula stages and in the maintenance of endodermal sox

212 cally in presumptive mesendoderm during late blastula stages and in the prechordal plate during late

213 ta5, which is expressed in the endoderm from blastula stages and show that its transcription is induc

214 luding the cells of the animal hemisphere at blastula stages and the neural plate border and neural c

215 wo cell populations are generated during the blastula stages by perpendicularly oriented divisions.

216 During the blastula stages Endo16 expression expands radially until

217 t levels throughout early development and at blastula stages enriched in the ventral side of the anim

218 afish bozozok (boz) locus is required at the blastula stages for formation of the embryonic shield, t

219 During blastula stages Xnr1, Xnr2 and Xnr4 are expressed in a d

220 ges, and also symmetrically during the early blastula stages, a period when heretofore largely unknow

221 is expressed only during cleavage and early blastula stages, and exclusively in micromeres.

222 disrupted synchronous cell divisions during blastula stages, apparently as a result of delayed progr

223 hat BMP4 mRNAs accumulate transiently during blastula stages, beginning around the 200-cell stage, 14

224 is also expressed in a gradient as early as blastula stages, but do not find any evidence of diffusi

225 ssed in the endodermal progenitors from late blastula stages, suggesting that it functions early duri

226 sh, fgf8 and ntl expression commences during blastula stages, whereas myogenesis, as indicated by myo

227 sms to repress BMP transcription during late blastula stages.

228 y present in the enveloping layer during the blastula stages.

229 nerates superficial ectoderm lesions at late blastula stages.

230 ion of the embryo during late cleavage/early blastula stages.

231 nd translation during the early cleavage and blastula stages.

232 oteins at the hoxb1a promoter already during blastula stages.

233 tween the late blastula and early mesenchyme blastula stages.

234 ential for repression of target genes during blastula stages.

235 sea urchin embryos during cleavage and early blastula stages.

236 gene is expressed in a dorsal region of the blastula that includes the extraembryonic yolk syncytial

237 s cells acquire apical-basal polarity in the blastula the protein becomes localized to the apical ext

238 regional identity of the animal cells of the blastula, the cells that are precursors of ectodermal st

239 In the Xenopus blastula, the cortical actin network in each blastomere

240 o-cell, which represents maternal RNA, early blastula, the time at which major tissue territories are

241 continuous activity of BMP antagonists from blastula through gastrula stages.

242 Nodal signals at stages ranging from the mid-blastula through the mid-gastrula.

243 at specify the adjacent marginal zone of the blastula to become mesoderm.

244 in the anterior neural ectoderm during late blastula to early gastrula stage in zebrafish.

245 atterning of the neural ectoderm in the late blastula to gastrula embryo in zebrafish.

246 factor genes (Erm, Pea3 and Er81) from early blastula to the 10 somite stage.

247 and is an essential function of Chk1 at the blastula-to-gastrula stage of development.

248 c transcription does not begin until the mid-blastula transition (MBT) 3 h after fertilization.

249 cur at the first mitotic cell cycle, the mid-blastula transition (MBT) and at gastrulation.

250 domain family, is expressed initially at mid-blastula transition (MBT) and during gastrulation in the

251 The Drosophila embryo at the mid-blastula transition (MBT) concurrently experiences a rec

252 ronous division and temporarily deferred mid-blastula transition (MBT) events.

253 specific satellite sequences during the mid-blastula transition (MBT) in Drosophila.

254 itiation of zygotic transcription at the mid-blastula transition (MBT) in the suppression of apoptosi

255 In Xenopus development the mid-blastula transition (MBT) marks a dramatic change in res

256 The Xenopus mid-blastula transition (MBT) marks the onset of large-scale

257 ne of the most prominent features at the mid-blastula transition (MBT) observed in most embryos is a

258 n developmental shift referred to as the mid-blastula transition (MBT) remain mysterious.

259 At the mid-blastula transition (MBT), externally developing embryos

260 At the mid-blastula transition (MBT), the cell cycle elongates and

261 genes activated in embryos prior to the mid-blastula transition (MBT), whereas multivalent genes are

262 ygotic gene expression occurs before the mid-blastula transition (MBT).

263 are activated after, but not before, the mid-blastula transition (MBT).

264 major embryonic transition known as the mid-blastula transition (MBT).

265 he maternal pool and increased after the mid-blastula transition (MBT).

266 ot express its downstream targets at the mid-blastula transition (MBT).

267 al changes in DNA methylation before the mid-blastula transition (MBT).

268 ture transcription activation before the mid-blastula transition and concomitant activation of a p53-

269 t zebrafish die by 4dpf, well beyond the mid-blastula transition at which transcription activates.

270 xis duplication but expression after the mid-blastula transition causes anterior truncation.

271 nine methylations are constrained to pre-mid blastula transition events in the embryo and therefore m

272 ryonic expression intersect indicating a mid-blastula transition from maternal to embryonic control o

273 ession increases substantially after the mid-blastula transition in a pattern mirroring that of activ

274 tivin-like signals do not act before the mid-blastula transition in zebrafish, but do have a variably

275 ygotic gene expression and underwent the mid-blastula transition normally.

276 irst expressed immediately following the mid-blastula transition on the dorsal side of the zebrafish

277 lular nutrition; development slowed, the mid-blastula transition was lost and maternal components bec

278 n factor XSiamois (which functions after mid-blastula transition).

279 n receptor inhibition continues past the mid-blastula transition, resulting in a progressive loss of

280 nt throughout development and, after the mid-blastula transition, was expressed in the head and in or

281 or other Activin-like ligands before the mid-blastula transition, when the dorsal axis is established

282 ls of zygotic gene activity prior to the mid-blastula transition.

283 ization in xp53 protein levels after the mid-blastula transition.

284 nd inactive, in embryos at cycle 14, the mid-blastula transition.

285 early embryonic stages leading up to the mid-blastula transition.

286 ces at the tenth cell cycle, marking the mid-blastula transition.

287 of Xenopus embryonic blastomeres by the mid-blastula transition.

288 RNAs that become degraded only following mid-blastula transition.

289 vage stages but returns to nuclei at the mid-blastula transition.

290 ality is observed around 3-4 hours after mid-blastula transition.

291 activation of the zygotic genome at the mid-blastula transition.

292 c surgical pairing of zebrafish embryos, and blastula transplantation assays, we show that HSCs emerg

293 Blastula transplantations determined that the effects of

294 h expression demonstrate that the mesenchyme blastula vegetal plate contains both animal/vegetal and

295 lular and extracellular matrix layers in the blastula wall of Strongylocentrotus purpuratus embryos a

296 l ECM as a major mechanical component of the blastula wall.

297 ts from the posterior region of stage XI-XIV blastulas were found to form heart muscle at high freque

298 iented divisions also take place in the frog blastula, where orientation of the spindle into the apic

299 ta-catenin signature genes were activated at blastula whereas others required the induction of endome

300 t during the first 24 h of development (late blastula), whole mount in situ hybridization was carried