ライフサイエンスコーパス: bleb

1 ession beyond the boundary of the subretinal bleb.

2 ents but to different degrees in the growing bleb.

3 lated with the size of the intradermal fluid bleb.

4 sion to account for the shape of a traveling bleb.

5 ccurs, and naturally gives rise to traveling blebs.

6 ed safely in eyes with functioning filtering blebs.

7 s concentrated kinase activity within leader blebs.

8 fficient to both induce and suppress nuclear blebs.

9 creased frequency of micronuclei and nuclear blebs.

10 asmic flow into protrusions forming membrane blebs.

11 e pseudopods or, unexpectedly, migrate using blebs.

12 roducing fluid pressure and the formation of blebs.

13 for the pathologies associated with nuclear blebs.

14 ime during actin cortex regrowth in cellular blebs.

15 ential characteristic for generating nuclear blebs.

16 include protruding structures termed nuclear blebs.

17 cilitate viral spread via infected apoptotic blebs.

18 palatal shelf development, and subepidermal blebbing.

19 ensive cell contraction followed by membrane blebbing.

20 Pearling thus differs from blebbing.

21 s them susceptible to toxin-induced membrane blebbing.

22 f target cells inhibits VLY-induced membrane blebbing.

23 ropel themselves, including by pseudopods or blebbing.

24 e RhoC signaling during cell contraction and blebbing.

25 a(2+)-dependent swelling (-80%) and membrane blebbing (-90%); 3) reduced calpain-dependent protein cl

26 formation of cellular protrusions in form of blebs, a type of protrusion found in various cell types.

27 The filtering blebs after phacoemulsification in the study group were

28 defects characterized by the development of blebs along the hair shaft in mice.

29 Cell contraction and blebbing also frequently occur as part of the cytopathic

30 nsible for the deregulation of the filtering bleb and subsequent loss of IOP control.

31 All patients showed flat filtering bleb and uncontrolled IOP (34.5 +/- 11 mmHg), under maxi

32 on of Krtap5-5 from cancer cells led to cell blebbing and a loss of keratins 14 and 18, in addition t

33 red for entotic invasion, which is driven by blebbing and a uropod-like actin structure at the rear o

34 , increase caspase activity, cause apoptotic blebbing and cell death, and finally induce coral bleach

35 ation, and AK-B knockdown can induce nuclear blebbing and cell death.

36 mains additionally induced cell rounding and blebbing and conferred enhanced sensitivity to osmotic s

37 sembled vesicles and, in some cases, promote blebbing and division.

38 um response factor) pathway for sustained PM blebbing and entotic invasion.

39 nhibition of Rac1 activity restored membrane blebbing and increased ROCK activity in Rap1GAP-depleted

40 ally and structurally distinct from membrane blebbing and involves disruption to the platelet microtu

41 the evagination occurs via a process akin to blebbing and is not driven by actin polymerization.

42 rop in cell height potentiated by persistent blebbing and loss of cortical F-actin homogeneity.

43 rigidity, which resulted in a dose-dependent blebbing and lysis of erythrocytes.

44 tion state is a major determinant of nuclear blebbing and morphology via its contribution to nuclear

45 echanism of proliferation involving membrane blebbing and tubulation, which is dependent on an altere

46 ision machinery and in occurring by membrane blebbing and tubulation.

47 Dictyostelium cells can move with both blebs and actin-driven pseudopods at the same time, and

48 Blebs and F-actin-driven pseudopods are alternative ways

49 minimal requirements of transitions between blebs and lamellipodia, as well as the time scales on wh

50 ing IOP in eyes with ischemic nonfunctioning blebs and patent trabeculectomy ostia.

51 reotypically produce first microspikes, then blebs and pseudopods only later.

52 er folate or cyclic AMP and moving with both blebs and pseudopods or with blebs only.

53 le to membrane tension in spatially coupling blebs and pseudopods, thus contributing to clustering pr

54 or dDia2 restores cells' ability to initiate blebs and thus migrate, though pseudopods are still lost

55 rosis and hypotony, a filtering conjunctival bleb, and focal scleromalacia with localized pigmentatio

56 ation, nanopore-conducted currents, membrane blebbing, and cell death.

57 ng, clathrin-mediated endocytosis, polarized blebbing, and endocytic vesicle sorting.

58 e in cellular Tropomyosin-3, plasma membrane blebbing, and release of 0.1- to 1-mum-diameter MPs.

59 ing under agarose, which efficiently induces blebbing, and the dynamics of membrane deformations were

60 lanoma cells from actin-driven protrusion to blebbing, and we present tools to quantify how cells man

61 roteomic analysis using cortex-rich isolated blebs, and a localization/small hairpin RNA (shRNA) scre

62 tines display villus fusion, apical membrane blebs, and disrupted microvilli.

63 gonistically to organize actin within leader blebs, and Erk mediates this effect.

64 lation of MMP14 into nascent plasma membrane blebs, and finally caspase- and MAPK-dependent apoptosis

65 l appearance of the blebs, using the Indiana Bleb Appearance Grading Scale classification.

66 Compared with baseline Indiana Bleb Appearance Grading Scale classifications, 2 eyes sh

67 ence (P<0.05) was noted between hypertensive bleb aqueous and controls in the amount TGF-beta2, inter

68 emotactic protein-1) in the glaucoma eye and bleb aqueous compared with controls.

69 Nuclear blebs are associated with both lamin and chromatin alter

70 type, and it has been reported that nuclear blebs are enriched in A-type lamins.

71 Blebs are pressure-driven cell protrusions implicated in

72 Blebs are pressure-driven protrusions that play an impor

73 These nuclear blebs are thought to be related to pathological gene exp

74 l nuclear morphology and protrusions termed "blebs" are diagnostic markers for many human afflictions

75 mean (SD) grades were 1.4 (0.1) for central bleb area, 1.4 (0.1) for bleb height, and 3.4 (0.2) for

76 Central bleb area, maximal bleb area, and bleb height were graded on a scale of 1 t

77 Central bleb area, maximal bleb area, and bleb height were grade

78 Using nuclear envelope (NE) blebbing as a phenotypic measure, we establish a direct

79 o membrane conformations and define membrane blebs as cellular compartments for direct interactions o

80 As previous studies have used blebs as reporters of membrane tension and pressure dyna

81 d in a 5-fold increase in surface-associated blebs, as well an onset of a wrinkled surface morphotype

82 ipodia, filopodia, invadopodia, and membrane blebs, as well as on cell-cell and cell-extracellular ma

83 The most common clinical settings were bleb associated (n = 17; 27%), after intravitreal inject

84 n-regulated MRTF transcriptional pathway for bleb-associated invasive motility, such as during entosi

85 related (eg, post-penetrating keratoplasty), bleb-associated, glaucoma drainage device-associated, an

86 ients identified, clinical settings included bleb association (n = 8), occurrence after cataract surg

87 loss of OM material through vesiculation and blebbing at cell-division sites and compensatory shrinka

88 ees C resulted in the appearance of membrane blebs at the poles and midpoint, prior to the formation

89 017) examine the membrane source that allows bleb-based cell migration in vivo.

90 and therefore suppresses efficient amoeboid bleb-based invasion.

91 Eps8 to enhance cortex tension and drive the bleb-based migration of cancer cells under non-adhesive

92 e actin cortex mechanics and thereby mediate bleb-based migration of cancer cells.

93 beyond the initial post-injection subretinal bleb boundary.

94 ed myotube apoptosis (widespread microscopic blebs, caspase 3/7 activation, and annexin V binding at

95 Blebbing cells are strongly chemotactic to cyclic-AMP, p

96 Recent experiments involving blebbing cells have led to conflicting hypotheses regard

97 IOP and bleb characteristics were compared on postoperative day

98 s, bleb morphology score using the Wuerzburg bleb classification score and any added glaucoma medicat

99 th only 20% of the silenced cells developing blebs compared with 53% of the control cells.

100 Bleb contents are trapped by the narrow neck that separa

101 -positive myofibroblasts, suggesting reduced bleb contraction.

102 This inhibition of cellular blebbing correlated with a 25% decrease in cytosolic fre

103 determined by FRET analysis both at cell and bleb cortices.

104 beyond the final boundary of the subretinal bleb could be detected.

105 njection site but within the confines of the bleb created by the intradermal bolus.

106 only 1 of the 40 eyes did a cystic avascular bleb develop, with all the other eyes being non-cystic i

107 s early as postnatal Day 3 (P3); thereafter, blebs devoid of actin or tubulin appeared at the region

108 of migration from primarily pseudopodial to bleb driven in <30 s.

109 ugh the contractile actin cortex involved in bleb-driven motility is well characterized, a role for t

110 e propose that mechanical resistance induces bleb-driven movement in Dictyostelium, which is chemotac

111 ontrols the ability of cells to contract and bleb during a variety of cellular processes, including c

112 a filtration procedures, and from 24 Molteno blebs during the hypertensive phase.

113 Ezrin is sufficient and important to sustain bleb dynamics for cell-in-cell invasion when SRF is supp

114 t also appears to have a higher incidence of bleb encapsulation.

115 ate in the direction of a very large 'leader bleb.' Eps8 bundling activity promotes cortex tension an

116 visual acuity (VA), mean deviation (MD), bleb evaluation, according to Moorfields Bleb Grading Sy

117 tokinesis, actin-dependent motility and cell blebbing, eventually abrogating gastrulation.

118 System (MBGS); spectral domain OCT (SD-OCT) bleb examination; number of glaucoma medications; freque

119 Blebs expand faster than pseudopods leaving behind F-act

120 ation propagate faster than the timescale of bleb expansion and that pressure equilibrates slower tha

121 ip among cytoplasmic rheology, pressure, and bleb expansion dynamics, and provide a more detailed pic

122 ty and elasticity of the cytoplasm determine bleb expansion time.

123 re equilibrates slower than the timescale of bleb expansion.

124 ilibration when compared to the timescale of bleb expansion.

125 mics have previously been used to understand bleb expansion.

126 re equilibrates faster than the timescale of bleb expansion.

127 cortex and is initially unsupported when the bleb extends as a result of cytosolic pressure.

128 leb height and 10 eyes showed an increase in bleb extension.

129 cy of trabeculectomy surgery with respect to bleb failure or IOP control was observed in both types o

130 ent abnormalities, and spontaneous filtering blebs (FDLAB, or Traboulsi syndrome).

131 OP, as well as the possibility that encysted blebs form cytokines.

132 role of physical forces in controlling where blebs form, and shows that in certain circumstances they

133 ; however, little is known about how and why blebs form.

134 escemet membrane (5.3%), hyphema (21.1%) and bleb formation (10.5%).

135 ng F-actin levels but blocking pseudopod and bleb formation and migration.

136 We induced bleb formation by disruption of the cortex and found tha

137 Theoretical modelling of bleb formation demonstrated that the increased stiffness

138 Bleb formation has been correlated with nonmuscle myosin

139 -IIC) have the same or differential roles in bleb formation is not well understood.

140 To test if bleb formation is related to bradykinin-promoted glioma

141 Rapid bleb formation may represent a conserved response of epi

142 mug/mL also inhibited cell growth and led to bleb formation on a fraction of the cells.

143 ongue with increasing sizes of intramyelinic bleb formation that could result in radial fractures of

144 compressive traction stresses, cells utilize bleb formation to indent the matrix in a protease indepe

145 an-specific receptor (hCD59) is required for bleb formation, as antibody inhibition of either toxin o

146 f the invaginations strongly interfered with bleb formation, cell motility, and the ability of the ce

147 IC1-GFP-expressing MCF-7 cells show multiple bleb formation, compared with 36% of cells expressing GF

148 nt background results in a stark increase of bleb formation, even though Tor2A does not respond to LA

149 ced susceptibility to mechanical and osmotic bleb formation, reduced migration and an increase in cel

150 of the meshwork could be a route to prevent bleb formation, which could be used as a potential thera

151 n and intracellular pressure to drive leader bleb formation.

152 cts of the meshwork could be responsible for bleb formation.

153 llowing the inflation of the membrane during bleb formation.

154 mpile an unbiased description of a "typical" bleb formed at the plasma membrane and quantify the effe

155 udies have relied heavily on recordings from blebs formed after axon transection, which may exaggerat

156 emotaxing Dictyostelium cells preferentially bleb from concave regions, where membrane tension facili

157 by the narrow neck that separates the leader bleb from the cell body.

158 ored group of marine particles (called micro-blebs) from the deep-water column.

159 vely, the cornea became clear, the filtering bleb functioned well, and IOP returned to normal values.

160 There was an insignificant difference in the bleb grading morphology regarding both groups (p = 0.35)

161 D), bleb evaluation, according to Moorfields Bleb Grading System (MBGS); spectral domain OCT (SD-OCT)

162 3 cytokines tested for, 19 were found in the bleb group, 14 in the glaucoma group, and 16 in the cont

163 her in the glaucoma group and highest in the bleb group.

164 directly confirm that the subsequent stop of bleb growth is induced by an increase of measured tensio

165 nsion is ~10-100 pN/mum and increases during bleb growth.

166 Nuclear blebs have been reported to be located in regions where

167 lassifications, 2 eyes showed an increase in bleb height and 10 eyes showed an increase in bleb exten

168 SD-OCT analysis denied differences in bleb height between MMC vs OLO (140.5 +/- 20.3 mu vs 129

169 Central bleb area, maximal bleb area, and bleb height were graded on a scale of 1 to 5 (1 indicati

170 4 (0.1) for central bleb area, 1.4 (0.1) for bleb height, and 3.4 (0.2) for bleb vascularity.

171 oid features such as cell rounding, membrane blebbing, high levels of contractility, and increased in

172 on the morphology and function of filtering bleb in patients after previous successful trabeculectom

173 r rigidity, which results in reduced nuclear blebbing in lamin B1 null nuclei.

174 ns, and VLY-induced epithelial cell membrane blebbing in the vaginal mucosa may play a role in the pa

175 as well as nonapoptotic plasma membrane (PM) blebbing in this cellular motile process.

176 NM-IIB-GFP can form filaments in blebs in 41% of NM-IIB-GFP-expressing cells, whereas fil

177 found in areas of dense pockets of apoptotic blebs in FcgammaRIII-KO.

178 served the rapid formation of large membrane blebs in human vaginal and cervical epithelial cells (VK

179 ne, is sufficient to predict the location of blebs in rounded cells moving in a highly resistive envi

180 erature-dependent diffusion on cell membrane blebs, in cells depleted of cholesterol, and upon acute

181 Cells can also migrate using blebs, in which the edge is driven forward by hydrostati

182 e cytokines in the aqueous from the encysted blebs, in which the IOP was the highest, suggests that t

183 retinal damage, particularly when SR vector bleb includes the fovea.

184 Cyclic-AMP induces transient blebbing independently of much of the known chemotactic

185 unction of Cdc42, and their unfolding allows bleb inflation and dynamic cell-shape changes performed

186 Because of their mechanical nature, blebs inform us about general cell-surface mechanics, in

187 oplasm shows that pressure disturbances from bleb initiation propagate faster than the timescale of b

188 ocular pressure (IOP) and the trabeculectomy bleb integrity, in a small series of eyes, both trabecul

189 A bleb is initiated when the cytoskeleton detaches from th

190 We find that cortical blebbing is tightly coupled to MRTF nuclear shuttling to

191 ion demonstrating that activation of MscL in blebs is identical to that in reconstituted bilayers.

192 es of membrane protrusions, such as multiple blebs, lamellipodia, combinations of both, or absence of

193 hieve this, we generate HEK293 cell membrane blebs largely free of cytoskeleton.

194 Bleb-related complications included bleb leak (n = 15), blebitis (n = 8), and hypotony (n =

195 d the occurrence of complications, including bleb leak, hypotony, hyphema, choroidal effusion, choroi

196 ry glaucoma or juvenile glaucoma, history of bleb leak, intraocular pressure sustained below the targ

197 Bleb leaks were observed in 59 cases (14%), 56 (95%) of

198 longer timescales that recapitulate the full bleb life cycle, including both expansion and healing by

199 M-II isoforms have differential roles in the bleb life cycle.

200 ctin-driven pseudopods at the same time, and blebs, like pseudopods, can be orientated by chemotactic

201 This excess is stored in a population of bleb-like protrusions (BLiPs), whose size distribution i

202 e migration is guided by bradykinin generate bleb-like protrusions.

203 ow PIEZO1 is activated by bilayer tension in bleb membranes, gating at lower pressures indicative of

204 Absence of bleb microcysts at 1 year, preoperative IOP, and number

205 lasticity and instigate a mode termed stable bleb migration in embryonic and tumor cells.

206 tion on intraocular pressure control and the bleb morphology in eyes that have undergone previous suc

207 wound, does not affect intraocular pressure, bleb morphology or function after one year of follow-up

208 Regarding the bleb morphology, the mean bleb morphology score in the temporal group was 10.50 +/

209 w-up regarding Intraocular pressure changes, bleb morphology score using the Wuerzburg bleb classific

210 raocular pressure and assessment of eventual bleb morphology variations in the follow-up period is ma

211 Regarding the bleb morphology, the mean bleb morphology score in the t

212 rabeculectomy and deterioration of filtering bleb morphology.

213 r revision for hypotony in 30 patients (7%), bleb needling in 71 patients (17%), and cataract extract

214 ding to IOP, use of hypotensive medications, bleb needling, and resuturing/revision for hypotony.

215 s at 1 year, preoperative IOP, and number of bleb needlings performed within the first year were sign

216 be cis mutations in TEK/Tie-2 in blue rubber bleb nevus and sporadic vascular malformations.

217 Blue rubber bleb nevus syndrome (Bean syndrome) is a rare, severe di

218 E2, in 15 of 17 individuals with blue rubber bleb nevus syndrome.

219 T1105N-T1106P is recurrent in blue rubber bleb nevus, whereas Y897C-R915C is recurrent in sporadic

220 totic features like cell shrinkage, membrane blebbing, nuclear condensation and DNA fragmentation.

221 ition, sAC inhibition reversed BSIA membrane blebbing, nuclear condensation, and DNA fragmentation.

222 ests that EPS-Si may be a precursor of micro-blebs observed in the deep ocean.

223 in-dependent membrane perturbations, such as blebs, observed during macropinocytosis, and activation

224 The model provides conditions under which blebbing occurs, and naturally gives rise to traveling b

225 misplacement and abnormal beating of cilia, blebbing of the microvilli.

226 ation of edge fluctuations in expanding cell blebs of filamin-deficient melanoma cells.

227 WCR, injury to the midgut was manifested by "blebbing" of the midgut epithelium into the gut lumen.

228 analysis revealed the presence of pores and blebs on mutant-Lkt-treated PMNs.

229 oving with both blebs and pseudopods or with blebs only.

230 rom mutant Krt75 mouse models reproduced the blebbing phenotype when grafted in vivo.

231 ransmembrane potential dissipation, membrane blebbing, phosphatidylserine exposure, DNA damage and ch

232 Blebs preferentially originate from negatively curved re

233 ssible expansion of the subretinal injection bleb prior to reattachment.

234 Unlike blebbing, procoagulant ballooning is irreversible and a

235 my to produce an even and diffuse filtration bleb, rather than the large incision sclerectomy propose

236 (+) and Cl(-) channels, which participate in bleb regulation.

237 ear risk of endophthalmitis (1.1%) and other bleb-related complications in the trabeculectomy cohort

238 Bleb-related complications included bleb leak (n = 15),

239 o trabeculectomy without the risk of serious bleb-related complications.

240 Bleb-related endophthalmitis developed in 1 patient with

241 is showed the significant risk factors for a bleb-related infection to be diagnoses of pigmentary gla

242 ion demonstrated a protective effect against bleb-related infections (P < .01) when risk factors were

243 d patients made aware of, the possibility of bleb-related infections long after trabeculectomy, espec

244 term follow-up demonstrates the incidence of bleb-related infections to be less than 2%, and describe

245 The Kaplan-Meier estimated incidence of bleb-related infections was 2.0% +/- 0.5% (mean +/- stan

246 Bleb-related infections were defined as blebitis, endoph

247 d describes the risk factors associated with bleb-related infections.

248 Twenty-four eyes were diagnosed with bleb-related infections; 15 eyes were found to have bleb

249 Genetically, blebbing requires myosin-II and increases when actin pol

250 y the effect of Arp2/3 complex inhibition on bleb retraction.

251 sin kinase II, which is necessary for proper bleb retraction.

252 e to 0.34 +/- 0.34 (P < .001) 6 months after bleb revision and to 0.45 +/- 0.55 (P < .001) 12 months

253 in outcome measure was final logMAR VA after bleb revision at 6 and 12 months.

254 Forty patients (14%) required bleb revision at least once.

255 h hypotony maculopathy who underwent primary bleb revision between June 1999 and September 2012 by a

256 Surgical bleb revision demonstrated a protective effect against b

257 , there was a higher unadjusted incidence of bleb revision in patients who had maculopathy (7.6 vs. 1

258 Surgical bleb revision is associated with good long-term control

259 Five eyes (15%) required a second bleb revision to correct persistent hypotony maculopathy

260 A protective effect of surgical bleb revision was demonstrated.

261 to 0.45 +/- 0.55 (P < .001) 12 months after bleb revision.

262 The fibrotic group had marked bleb scarring and vascularization and worse logMAR visua

263 Strikingly, numerous "bleb" shaped microprojections were observed extending fr

264 es or against mechanical resistance, but how bleb sites are selected and directed to the cell front r

265 Here we ask how bleb sites are selected and how the two forms of project

266 f the cytoplasm relieves pressure and limits bleb size, and that both permeability and elasticity of

267 ly localizing components of the omega-shaped bleb structure.

268 felt produced too large and thin a filtering bleb subject to complications.

269 A physical model of blebbing suggests that detachment of the cell membrane i

270 ogy and composition between EPS-Si and micro-blebs suggests that EPS-Si may be a precursor of micro-b

271 We also find that traveling blebs tend to expand in all directions unless otherwise

272 The introduced technique shows that the bleb tension is ~10-100 pN/mum and increases during bleb

273 utant had growth defects and formed membrane blebs that led to cell lysis when GlcNAc was replaced by

274 the flanks of either extending pseudopods or blebs themselves.

275 ent under agarose and cells that produce few blebs, though actin polymerization appears unaffected.

276 cancerous single-cells transitioning from a blebbing to a pseudopodial morphology.

277 he confines of tissues, cancer cells can use blebs to migrate.

278 Cells often employ fast, pressure-driven blebs to move through tissues or against mechanical resi

279 The model predicts conditions under which blebs travel or remain stationary, as well as the bleb t

280 travel or remain stationary, as well as the bleb traveling velocity, a quantity that has remained el

281 Furthermore, PM blebbing triggered SRF-mediated up-regulation of the met

282 to cyclic-AMP, producing nearly all of their blebs up-gradient.

283 esurgical and postsurgical appearance of the blebs, using the Indiana Bleb Appearance Grading Scale c

284 Bleb vascularity was graded on a scale of 1 to 5 (1 indi

285 1.4 (0.1) for bleb height, and 3.4 (0.2) for bleb vascularity.

286 Dose-dependent blebbing was also observed during microscopic analysis.

287 Blebs were smaller, thicker, and less ischemic in AgNP-t

288 -associated envelope abnormalities, that is, blebs, were confirmed by electron microscopy and immunof

289 15 patients, with otherwise well functioning blebs, were presented with uncontrolled IOP, in a variab

290 witch from using predominantly pseudopods to blebs when migrating under agarose overlays of increasin

291 ape transformations that is complementary to blebbing, where the plasma membrane detaches from the ac

292 e each measurement before the cell undergoes blebbing, which is associated with a considerable increa

293 ost prominently in the formation of membrane blebs, which were shown to colocalize with incoming vira

294 tours, we use cell geometry to predict where blebs will form in migrating cells.

295 on should focus on generating an 8- to 10-mm bleb with each injection.

296 d and sustained reduction of IOP and promote blebs with decreased fibrosis and ischemia as well as in

297 All eyes had ischemic nonfunctioning blebs with patent internal ostia and underwent Nd:YAG LG

298 expression also affected nsPEF-induced cell blebbing, with only 20% of the silenced cells developing

299 Six (30%) cystic thin avascular blebs without oozing were recorded in the MMC group and

300 atin results in a softer nucleus and nuclear blebbing, without perturbing lamins.