ライフサイエンスコーパス: blot

1 p-STAT3 levels were measured using Western blot.

2 ing immunohistochemical staining and western blot.

3 the TRIM proteins were confirmed by Western blot.

4 n wild-type (WT) protein detected by Western blot.

5 psonophagocytic inhibition assay and Western blot.

6 or confirmation by flow cytometry or Western blot.

7 showed elevated 4-repeat isoforms by western blot.

8 cal comparison was carried out using Western blot.

9 rotein expression using western blot and dot blot.

10 Selected proteins were validated by Western blot.

11 essed by histological techniques and western blot.

12 re confirmed at the protein level by Western blot.

13 nly gp160 antibodies detected in the western blot.

14 as evaluated by quantitative PCR and Western blot.

15 ered testing, obviating the need for Western blots.

16 similar to small RNA sequencing and northern blots.

17 red by qPCR, immunocytochemistry and western blots.

18 s are higher-specificity IgG and IgM Western blots.

19 ts sequentially, without the need of Western blots.

20 e selected for further validation by western blotting.

21 vented seroconversion, as shown with western blotting.

22 ein level by confocal microscopy and western blotting.

23 P) receptor expression determined by Western blotting.

24 ptor 4 (TLR-4) protein expression by Western blotting.

25 osphorylation of Chk1 and H2AX using Western blotting.

26 ation was analysed, and confirmed by Western blotting.

27 2 and Bax, respectively, revealed by Western blotting.

28 rs were measured by qPCR, ELISA, and Western blotting.

29 0A antibody immunohistochemistry and Western blotting.

30 ood-stage parasites was confirmed by western blotting.

31 chemokine gradients was supported by Western blotting.

32 oxide synthase levels determined by Western blot, 3-nitrotyrosine and 4-hydrpxnonenal both assayed b

33 Classification of IOH was preretinal (57%), blot (57%), dot (38%), flame-shaped (16%), and vitreous

34 nd an additional 4% were positive on western blot alone.

35 qPCR,luciferase reporter assays, and western blot also verified the ATP synthase subunit genes ATP5G1

36 P2X7R (HEK293-hP2X7R) and underwent Western blotting, an immunofluorescence assay, and radioimmunohi

37 Western blot analyses confirmed the involvement of Sirt2 inhibit

38 atic, luciferase reporter assay, and Western blot analyses identified RhoA as a target of miR-31-3p i

39 ults with flow cytometry, ELISA, and Western blot analyses in an additional 39 patients and 22 contro

40 Western blot analyses indicated that p38a, STAT1, STAT3, CREB1,

41 Immunohistochemical staining and Western blot analyses showed increased EZH2 expression in human

42 Western blot analyses showed that the loss of LKB1 and phosphata

43 Immunohistologic and Western blot analyses were performed to evaluate NIS protein exp

44 unofluorescent stainings, as well as Western blot analyses, on cervical and lumbar sections of the sp

45 fter portal reperfusion, followed by Western blot analyses.

46 sacrifice and immunohistochemical or western blot analyses.

47 sed by immunofluorescence assays and Western blot analyses.

48 Sixty patient samples underwent Western blot analysis (15 young, 24 aged, and 21 with AD).

49 rts for the presence of THSD7A-Ab by Western blot analysis and a newly developed indirect immunofluor

50 Southern blot analysis and Droplet Digital PCR revealed that line

51 ators of autophagy was measured with Western blot analysis and fluorescence microscopy.

52 Western blot analysis and gene expression profiling showed that

53 lasma and Lp(a) uptake studied using Western blot analysis and intracellular localization of Lp(a) by

54 ets of miRNA-31-3p were confirmed by Western blot analysis and luciferase reporter assay.

55 Here we used far-Western blot analysis and microscale thermophoresis to show that

56 Quantitative PCR and western blot analysis confirmed loss of Bach2 in c-Rel mutant Em

57 Western blot analysis confirmed loss WIF1 expression and activat

58 ein vesicles showed weak binding, and ligand blot analysis confirmed the binding specificity.

59 Western blot analysis confirmed the increase in GluA1 expression

60 g study with specific inhibitors and Western blot analysis demonstrated that CTGF-induced expression

61 Furthermore, western blot analysis demonstrated that LCN stimulations signifi

62 Dot blot analysis demonstrated that nonfibrillar Abeta oligo

63 Western blot analysis demonstrated UT-A1 in the inner medulla of

64 Northern blot analysis detected the siRNAs products in virus-free

65 Western blot analysis indicated that the protein expression leve

66 Western blot analysis indicated that Wnt7a induced both canonica

67 The Western blot analysis indicates the downstream effectors of inte

68 d beta-Galactosidase staining and by Western blot analysis of p16.

69 by 72 h on immunohistochemistry, and Western blot analysis of P2X7R for saline- and lipopolysaccharid

70 On Western blot analysis of pathway checkpoints, p-mTOR (p=0.03) an

71 Western blot analysis of the basolateral amygdala (BLA) revealed

72 Western blot analysis of total cell lysate obtained from normal

73 Likewise, western blot analysis revealed a 41% clearance of soluble tau (3

74 Although Western blot analysis revealed no difference in opsin expression

75 Western blot analysis revealed that mTOR deletion led to decreas

76 Western blot analysis revealed that Notch2HCS mutant splenocytes

77 Western blot analysis showed that both phosphorylated STAT3 and

78 DNA gel blot analysis showed that the T-DNA inserts are stable;

79 icular, live cell Ca(2+) imaging and Western blot analysis showed that TRAM-34 pretreatment decreased

80 oyed immunofluorescence staining and Western blot analysis to assess the expression of autophagy-rela

81 by pathway inhibitor experiments and Western blot analysis to be mediated via MEK-ERK signaling.

82 This study used western blot analysis to compare protein levels of tyrosine hydr

83 c inhibitors, phosphoproteomics, and Western blot analysis to define the necessity of various protein

84 ions coupled with flow cytometry and Western blot analysis to elucidate the function and structural b

85 Cellular fractionation and Western blot analysis were used to measure the levels of histone

86 ssed by immunofluorescence staining, Western blot analysis, and ELISA.

87 fected cells was examined by RT-PCR, western blot analysis, and immunohistochemistry.

88 Western blot analysis, as well as characterization of the bindin

89 Ligand blot analysis, C3 binding experiments and opsonophagocyt

90 evel of target protein expression by Western blot analysis, platelet aggregation using an aggregomete

91 January 2007 to March 2014) included Western blot analysis, pulsed-field gel electrophoresis (PFGE),

92 determined by luciferase assays and Western blot analysis, reversion-inducing cysteine-rich protein

93 Compared with Western blot analysis, the IFT had a 92% sensitivity and a 100%

94 In particular, we examined, using Western blot analysis, the levels of H3K36me3 and H4K16ac in cel

95 Using Western blot analysis, we confirmed that treatment of HS rats wi

96 Through western blot analysis, we identified that human glioma cells tha

97 n duodenum by immunofluorescence and Western blot analysis.

98 surface biotinylation and subsequent Western blot analysis.

99 om human and mouse models of DCM via Western blot analysis.

100 resence of antiretinal antibodies by Western blot analysis.

101 of the expected molecular weight in western blot analysis.

102 a third of which were validated by northern blot analysis.

103 cid transporter 3) were validated by Western blot analysis.

104 of EGFR downstream signalling in the western blot analysis.

105 T-PCR, and proteins were detected by Western blot analysis.

106 as measured by two-dimensional (2-D) Western blot analysis.

107 Western blotting analysis of PELP1-cyto HMECs showed up-regulati

108 A Western blotting analysis revealed that biotin-labeled beauverio

109 time quantitative PCR (RT-qPCR), and Western blotting analysis.

110 econdary structure were confirmed by Western Blot and Circular Dichroism (CD), respectively.

111 l-surface cross-linking, followed by Western blot and coimmunoprecipitation.

112 r viral oncoprotein expression using western blot and dot blot.

113 Dot blot and ELISA assays demonstrate the inhibition of the

114 OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni affinity c

115 ments under sonication, by SDS-PAGE, western blot and ELISA, with serum IgE of allergic individuals,

116 mediated cell signaling, such as the western blot and flow cytometry, are limited in three aspects: 1

117 all interfering RNA library based on western blot and identified SCF-FBXO32 to be a new E3 ligase, wh

118 IgE-western blot and IgE-ELISA were complemented by Skin Prick Testi

119 Second, western blot and immunohistochemical analysis confirmed that Sal

120 should have a 2-tier design and that Western blot and immunohistochemistry should be the methods used

121 XII (CA12), at the protein level by western blot and immunohistochemistry.

122 ersus muscle tissue as determined by Western blot and immunohistochemistry.

123 Real time PCR, western blot and knock down assays demonstrate that IL-27 is abl

124 ll nuclear antigen were evaluated by Western Blot and liver injury was assessed by ALT and histology.

125 en, defined in mouse brain lysate by Western blot and mass spectrometry, was confirmed by dual immuno

126 Western blot and mRNA expression analyses in mice yielded consis

127 Western blot and RT-PCR were performed to quantitate Hsp90 pathw

128 The MRM UHPLC-MS/MS method, Western blot and RT-PCR were used along with SULT activity measu

129 We confirmed, using western blot and spectrophotometry, that Hsp90 or BRAF inhibitor

130 tion, immunohistochemistry, RT-PCR, northern blot and western blot experiments.

131 tion, which was further confirmed by western blot and/or qPCR.

132 pressed N-terminal LRP2 fragments on Western blots and immunoprecipitated the recombinantly expressed

133 alidate novel RNA transcripts using Northern blots and luciferase promoter fusions.

134 Western blots and proteomic analysis corroborated the inverse re

135 3 (LC3), an autophagosome marker, by Western blotting and confocal fluorescence microscopy.

136 ophysiological characterization, and Western blotting and confocal imaging to assay expression and su

137 dies and analyzed via Iba1 and P2X7R Western blotting and Iba1 immunohistochemistry.

138 Western blotting and immunofluorescence were used for evaluating

139 Trx1 levels as reflected by RT-PCR, Western blotting and immunohistochemistry analyses.

140 Western blotting and immunostaining analysis confirmed that ShcA

141 Western blotting and molecular docking studies suggested that th

142 Western blotting and MS results indicated that rHBeAg and HBeAg

143 Western blotting and quantitative PCR analysis of infected cells

144 Western blotting and Real-time PCR were used to assess the expre

145 ciferase assays, immunofluorescence, western blotting and virus infection.

146 LSE samples (quantitative RT-PCR and Western blotting) and in normal and AE skin biopsy specimens (im

147 uromycin incorporation experiments, Northern blot, and electron microscopy analyses demonstrated the

148 ysis was performed via RNA analysis, Western blot, and histology.

149 scriptase-polymerase chain reaction, Western blot, and immunohistochemistry were used to analyze secr

150 ed by chromatin immunoprecipitation, Western blot, and immunostaining.

151 In cord blood, qPCR, TESA-blot, and micromethod sensitivities/specificities were 6

152 was confirmed with luciferase assay, western blot, and NF-kappaB analysis.

153 Immunohistochemistry, Western blot, and qRT-PCR were performed on the outflow vein at

154 reverse transcription-PCR (RT-PCR), Western blot, and secretome analyses established that S. Paratyp

155 Our blocking studies, western blot, and tissue histological imaging suggested that the

156 scent in situ hybridization (FISH), Northern blots, and RNA sequencing-implicates MRP RNA in pre-rRNA

157 proximately 41-kDa protein (spx1) on Western blots, and the signal was efficiently blocked by the com

158 gation, immunoprecipitation, dot and Western blotting, and confocal imaging were performed for signal

159 enomic RNA strands were detected by Northern blotting, and crystalline lattices of viral particles of

160 ve electron microscopic morphometry, Western blotting, and functional tests.

161 paracellular flux, mRNA expression, Western blotting, and immunofluorescence staining.

162 evaluated by means of real-time PCR, Western blotting, and immunohistochemistry.

163 re sampled for immunohistochemistry, western blotting, and mitochondrial high-resolution respirometry

164 cells were evaluated by using ELISA, Western blotting, and peptide epitope mapping.

165 ar pathways was assessed by means of Western blotting, and the final outcome on immunomodulatory prop

166 scription polymerase chain reaction, Western blotting, and zymography.

167 t assay (ELISA), flow cytometry, and Western blot are common bioanalytical techniques.

168 Western blots are also complex, difficult to interpret, and rela

169 to an increase in monomeric Cav1 in Western blots, as well as a simultaneous increase in vesicle num

170 Further analyses using western blot assays showed that the FOXA2 p.(S169P) variant is p

171 sequencing technology), and quantitative dot blot assays.

172 '-UTR luciferase reporter assays and Western blot assays.

173 pomastigote excreted-secreted antigen (TESA)-blots at birth and 1 month and by IgG serology at 6 and

174 A novel dot blot-based assay system for the detection of IgE against

175 roarray, quantitative real-time PCR, Western blotting, chromatin immunoprecipitation, and functional

176 n vivo fluorescence observations and Western blots confirmed MzASMT9 was localized in the chloroplast

177 Quantitative PCR (qRT-PCR) and Western blotting confirmed changes in expression of seven of the

178 coactivator of ACTA2 Two-dimensional Western blotting confirmed induction of both wild-type and mutan

179 erior affinity reagents suitable for western blotting, confocal fluorescence microscopy and pull-down

180 sed P. aeruginosa adhesion to MyD88(-/-) and blotted corneas is not due to reduction in total surface

181 The Western blot data shown for p-ERK1/2 and actin are not from this

182 Size exclusion chromatography and Western blotting data obtained by using purified recombinant E1/

183 Flow cytometry and Western blot demonstrated that lipoglycans from M. tuberculosis-

184 The new dot blot detection system resolved individual IgE sensitizat

185 When using northern blotting during these studies, we discovered that miRNA-

186 fibroblasts and characterized using Western blot, electron microscopy and flow cytometry.

187 ngs suggest that the developed MAb based dot blot ELISA is a simple, rapid performed in less than 8 h

188 included for the evaluation of MAb-based dot blot ELISA.

189 sical assays including amyloid kinetics, dot blot, ELISA, and TEM show that 5 effectively inhibits bo

190 We used qRT-PCR, Western blotting, ELISA, and ChIP (chromatin immunoprecipitation

191 netic experiments (quantitative PCR, Western blot, EMSA) or genome-wide assays (RNA-sequencing, ChIP-

192 te simultaneously and independently within a blot, enabling straightforward multiplexing.

193 Northern blotting established that HCV dsRNA contained genome-len

194 GATA4 in nucleus were detected with Western blot experiment.

195 luated in ELISA, flow cytometry, and Western blot experiments and compared to analogous experiments u

196 chemistry, RT-PCR, northern blot and western blot experiments.

197 were tested for reactivity to nitrocellulose-blotted extract from purified HDM bodies, and the IgE-re

198 well as for its detection by ELISA, Western blot, flow cytometry, and confocal microscopy.

199 Here we describe a microfluidic western blot for an eight-plex protein panel for individual CTCs

200 Subsequently, Western blot for the autophagic structure LC3-I and LC3-II (micr

201 3b-II/LC3b-I ratio, were detected by Western blotting from whole retinal extracts.

202 and traffic by confocal microscopy, western blotting, gel filtration, and immunoprecipitation studie

203 ssay (VOPBA) in combination with far-Western blotting, gradient-purified EAV particles were shown to

204 and E. coli antigens were detected in immune-blotted HDM extract, and the presence of IgE-reactive an

205 s beading, whereas those with 4-quadrant dot-blot hemorrhages (4Q DBH) had 3.84 higher HR of developi

206 linked immunosorbent assays (ELISA), Western blot, high performance liquid chromatography (HPLC), spe

207 copy number is estimated by either Southern blot hybridization analyses or quantitative polymerase c

208 action (PCR), real time PCR (RT-PCR) and dot blot hybridization have also been proposed for pathogen

209 In the present study, Western blotting identified a band corresponding to C3 in freshl

210 munosorbent assays (ELISA) and direct tissue blot immunoassays (DTBIA).

211 lyp tissue were analyzed by means of Western blotting, immunoassays, or histology.

212 uced autophagosomes was confirmed by Western blotting, immunofluorescence microscopy, and immunoelect

213 ere validated by immunoprecipitation/Western blotting, immunofluorescence, and proximity ligation ass

214 Using western blotting, immunofluorescence, ELISA and qRT-PCR, we inve

215 We used western blots, immunohistochemistry, H&E and biochemical enzyme

216 established and then validated using western blotting, immunohistochemistry, and comparable TCGA RPPA

217 Using molecular cloning, RT-PCR, Western blotting, immunolocalization and in vitro functional ass

218 ime polymerase chain reaction, (far) Western blot, immunoprecipitation, and immunocytochemistry were

219 dimer and ENG were analyzed also by Western blot in ascending aorta samples from other 10 tricuspid

220 y inadvertently inserted images of the wrong blots in several of the figures, resulting in the duplic

221 nerated and verified using q-PCR and western blotting in human keratinocytes (HaCaT) and endothelial

222 ochemistry on kidney sections and by Western blotting in plasma samples from rats subjected to renal

223 strate their superior performance in Western blotting, in both peroxidase- and fluorophore-linked for

224 chain reaction, flow cytometry, and Western blotting-in several nonprostatic cell lines and xenograf

225 However, direct quantification via western blots indicates that the actin expression is the same ac

226 surface glycosylation, and for tissue paper blotting is likely due to cell permeabilization.

227 Western blotting is most often used for protein identification o

228 This improved northern blotting is particularly useful to analyze products of R

229 lysosome compartment evaluation, and western blotting light chain 3 (microtubule-associated protein 1

230 Parallel western blot, luciferase reporter and gene target assays identif

231 hich survived EBOV challenge, ELISA, western blot, mass spectrometry and flow cytometry were used to

232 tion by quantitative Western and far-Western blotting, mass spectrometry, and computational modeling.

233 histochemistry and HIF-1alpha/2alpha Western blot/messenger RNA analysis of inflamed and healthy ankl

234 Casein zymography and Western blot of m-calpain were performed using the water soluble

235 Moreover, immunostainings and dot blots of optic nerve and myelin showed that expression o

236 Western blotting of MKK2 protein and its T31 phosphorylated pept

237 To address this question, we used Western blotting of postmortem tissue from human V1 (12 female,

238 t complement RNA-seq, discover that northern blotting of small RNAs is biased against short sequences

239 ependent MGMT silencing confirmed by Western blotting of tumor tissue ex vivo.

240 Western-blotting on mouse cervix confirmed large scale histone d

241 le either being positive on standard western blot or harboring HDV RNA detectable by real-time quanti

242 lly confirmed individuals with AD by Western blot or immunofluorescence for AQP4, amyloid-beta 1-42,

243 ression as judged by flow cytometry, Western blot or immunofluorescence.

244 ISA (IL-6, IL-1beta, and CXCL2), and Western blot or immunostaining (CEBP, STAT3, NUPR1, and IGF1) sh

245 or spleen of these animals by either Western blotting or after amplification by serial PMCAb.

246 tative label-free mass spectrometry, Western blotting, or confocal imaging.

247 mmunoglobulin M and immunoglobulin G Western blots, performs well in late-stage LD but is insensitive

248 The HCR northern blot protocol takes approximately 1.5 days independent o

249 ed in detection of miRNAs including northern blotting, quantitative real time PCR (qRT-PCR) and micro

250 l outcomes were assessed by means of Western blotting, real-time PCR, differentiation, and proliferat

251 le analysis, morphologic assessment, Western blotting, receptor binding, gene expression, small inter

252 Tissue paper blotting removed GalNAz-labeled surface cells, causing D

253 etected with quantitative RT-PCR and Western blotting, respectively, in both platelets and Meg-01 cel

254 MS of material collected on the tissue paper blots revealed 67 GalNAz-labeled proteins, including int

255 Immunofluorescence microscopy and western blotting revealed marked disorganization and reduced COL

256 IHC and western blotting revealed reduction in epidermal hyperplasia (Ki

257 with real-time quantitative PCR and Western blotting revealed up-regulation of C3 and CFB expression

258 s, supplemented by real-time PCR and Western blotting, revealed that the expression of critical regul

259 determined using immunofluorescence, western blot, reverse-transcriptase PCR, chromatin immunoprecipi

260 assays, quantitative real-time PCR, western blots, scratch assays, CCK-8 assays and tubule formation

261 in peptides), but quantitation using western blots showed 6.3- to 8-fold or 91- to 125-fold increase

262 ched non-transgenic littermates, and western blots showed increased lysosomal proteins including LAMP

263 Protein blots showed that ADHE is regulated under oxic condition

264 Western blots showed that the triceps surae muscles of 'ligated'

265 uencing, quantitative real-time PCR, Western blotting, small interfering RNA interference, and kinase

266 Western blotting suggested beta-conglutins were the main protein

267 Using mass spectrometry and Western blotting techniques, we have identified choline transpor

268 igen and antibody to 65 days for the Western blot test.

269 confirmed by immunofluorescence and western-blot that rod degeneration in CERKL-/- zebrafish occurre

270 lution, we used deep sequencing and Northern blots to isolate caste-associated miRNAs in the primitiv

271 from an "enclosed" microchannel for PAGE and blotting to an "open" PA gel lane for immunoprobing and

272 tative polymerase chain reaction and Western blotting to investigate changes in ion channel expressio

273 ajor antigenic region was defined by Western blot using recombinant protein fragments.

274 AcMod binding and oligomerization by western blots using brush border membrane vesicles (BBMV) from a

275 igomers, whereas immunoprecipitation-Western blotting using high-detergent extracts revealed a variet

276 cipitation and simultaneous assay by Western blotting using multiplex fluorescence imaging with speci

277 pectrometry, and further verified by Western blotting using specific Abs.

278 These were further validated by Western blotting using specific Abs.

279 c for all five tested SULTs, whereas Western blot was specific for only two isoforms.

280 At the same time Western blotting was performed with the patient's serum of previ

281 transfection with miR-146b mimic and western blotting was used to analyse SOX5.

282 e determined by neutral comet assay; western blotting was used to evaluate protein changes; changes i

283 mercial sources were investigated by western blot (WB) and in multiple research grade PSA ELISAs.

284 J protein expression was assessed by Western blotting (WB) and immunocytochemistry (ICC).

285 ssociated prion protein (PrP(Sc)) by Western blotting (WB), antigen capture enzyme immunoassay (EIA),

286 n immunochemical techniques, such as western blotting (WB).

287 Based on lipid analysis and Western blotting, we show that the bacteria-like entities consis

288 tochemistry, immunofluorescence, and Western blot were used to validate apoptosis by cleaved caspase-

289 PCR and Southern blots were performed on DNA extracted from the brain tis

290 Western blots were used to quantify the abundance of the CO2 -fi

291 ceptor (GHRH-R) measured by qPCR and Western blotting were significantly down-regulated in retinas of

292 l-time polymerase chain reaction and Western blotting were used to evaluate Renal Klotho expression.

293 and m-calpain level (as assessed by Western blot) were all significantly higher in the NACA-treated

294 ived from other techniques (such as Northern blots) were used as reference genes in RT-qPCR experimen

295 tients had antiretinal antibodies on Western blot with an average of 6.6 bands.

296 2D gel electrophoresis, followed by Western blot with pooled or individual sera.

297 Allergen detection in 2D-Western blots with PBS resulted in greater sensitivity than with

298 We performed Western blotting with enokitake extracts and the patient's serum

299 nse gene 88), or after superficial injury by blotting with tissue paper.

300 (n = 20)) by automated quantitative western blotting, with excellent agreement with our proteomics f