ライフサイエンスコーパス: blotting

1 ADAMTS13 antibody was detected by Western blotting.

2 ix (LYPAL), by mass spectrometry and Western blotting.

3 detected by immunohistochemistry and western blotting.

4 tative polymerase chain reaction and Western blotting.

5 measured by immunohistochemistry and Western blotting.

6 um samples was detected by ELISA and Western blotting.

7 ctions by co-immunoprecipitation and Western blotting.

8 acid (HOCl)-induced SSc by ELISA and Western blotting.

9 psy and loss of calpain 3 protein on western blotting.

10 ted T-cells (SOFAT) was evaluated by Western blotting.

11 ges in Hsp levels were determined by Western blotting.

12 e detected in CCD case cerebellum by western blotting.

13 2 and Bax, respectively, revealed by Western blotting.

14 enes, were determined by RT-qPCR and Western blotting.

15 sphorylation of p70 S6 kinase, using Western blotting.

16 profiled using lectin microarrays and lectin blotting.

17 often detected as multiple bands by western blotting.

18 y pathway proteins were validated by western blotting.

19 ion by exome sequencing, RT-PCR, and Western blotting.

20 rs were measured by qPCR, ELISA, and Western blotting.

21 of repeat reactive samples by HIV-1 Western blotting.

22 hypoxia-inducible factor 1-alpha by Western blotting.

23 2 phosphorylation was measured using Western blotting.

24 koids exposed to Mn deficiency after western blotting.

25 h a mouse anti-BmAMA1 antibody using Western blotting.

26 0A antibody immunohistochemistry and Western blotting.

27 ng flow cytometry, real-time PCR, and immune-blotting.

28 say, immunofluorescent staining, and Western blotting.

29 ssion were successfully measured via Western blotting.

30 tion polymerase chain reaction, and Northern blotting.

31 , p.Glu134* and Delta exons 9-14) by western blotting.

32 ood-stage parasites was confirmed by western blotting.

33 ional proteomics and two-dimensional Western blotting.

34 r translocation were quantified with Western blotting.

35 oteins of interest were validated by Western blotting.

36 nd nine microRNAs were confirmed by northern blotting.

37 cell lysis, gel electrophoresis and western blotting.

38 ncing, quantitative PCR (qPCR), and Northern blotting.

39 of the AMPA receptor, measured with western blotting.

40 validated by quantitative RT-PCR and Western blotting.

41 e synthase pathways were assessed by Western blotting.

42 473) phosphorylation was analyzed by Western blotting.

43 nd cellular localization examined by Western blotting.

44 ed with protein expression levels by Western blotting.

45 g, elastica van Gieson staining, and Western blotting.

46 arginine methylation was assessed by Western blotting.

47 iptase polymerase chain reaction, or western blotting.

48 chemokine gradients was supported by Western blotting.

49 e selected for further validation by western blotting.

50 vented seroconversion, as shown with western blotting.

51 ein level by confocal microscopy and western blotting.

52 P) receptor expression determined by Western blotting.

53 ptor 4 (TLR-4) protein expression by Western blotting.

54 osphorylation of Chk1 and H2AX using Western blotting.

55 ation was analysed, and confirmed by Western blotting.

56 ft assay, coimmunoprecipitation, and western blottings.

57 l-time polymerase chain reaction; 2) Western blotting; 3) fluorescence-activated cell sorting; 4) imm

58 Quantitative Western blotting allowed us to estimate that mouse thymocytes co

59 P2X7R (HEK293-hP2X7R) and underwent Western blotting, an immunofluorescence assay, and radioimmunohi

60 e transcriptase quantitative PCR and western blotting analyses, we confirm the direct regulation of C

61 microarray expression profiling and Western blotting analysis identified preferential phosphorylatio

62 Western blotting analysis of PELP1-cyto HMECs showed up-regulati

63 A Western blotting analysis revealed that biotin-labeled beauverio

64 enerative process, by real time PCR, Western blotting analysis, and immunohistochemistry.

65 ed using both immunofluorescence and Western blotting analysis, and nuclear functional TDP-43 was mea

66 a, but normal PKCzeta expression, by Western blotting analysis, and vice versa.

67 re, using whole-cell patch clamp and Western blotting analysis, we demonstrate that the hERG/IKr chan

68 time quantitative PCR (RT-qPCR), and Western blotting analysis.

69 immunoreactive tryptic fragments by Western blotting and analyzed them by mass spectrometry.

70 3 (LC3), an autophagosome marker, by Western blotting and confocal fluorescence microscopy.

71 ophysiological characterization, and Western blotting and confocal imaging to assay expression and su

72 Western blotting and cytochemistry used Siglec-F-Fc as a probe f

73 in vitro by using flow cytometry and Western blotting and ex vivo by using RT-PCR after laser microdi

74 Northern blotting and fluorescence microscopy indicate that ORFX

75 Western blotting and fluorescent resonance energy transfer assay

76 mparison subjects was analyzed using Western blotting and Golgi histochemistry to examine the hypothe

77 ymerase chain reaction (qRT-PCR) and western blotting and hepatic stellate cell (HSC)-MF contractilit

78 dies and analyzed via Iba1 and P2X7R Western blotting and Iba1 immunohistochemistry.

79 Western blotting and immunofluorescence analysis demonstrated ne

80 osphorylation, as identified through Western blotting and immunofluorescence microscopy.

81 he effect of AZD6738 was assessed by western blotting and immunofluorescence of key DDR proteins.

82 Western blotting and immunofluorescence were used for evaluating

83 cted by means of immunoprecipitation/Western blotting and immunofluorescence.

84 was determined by ELISA, zymography, Western blotting and immunofluorescent staining.

85 Trx1 levels as reflected by RT-PCR, Western blotting and immunohistochemistry analyses.

86 Western blotting and immunohistochemistry showed decreases in be

87 qPCR, Western blotting and immunohistochemistry were performed.

88 vity in the hippocampus, measured by Western blotting and immunohistochemistry, was increased in a ra

89 Using a combination of Western blotting and immunohistochemistry, we identified an incr

90 STAT3 and NFkappaB were detected by Western blotting and immunoprecipitation, respectively.

91 Western blotting and immunostaining analysis confirmed that ShcA

92 C was determined via combined use of western blotting and immunostainings.

93 n of N-glycan core fucosylation using lectin blotting and lectin ELISA assays.

94 roscopy of isolated nucleoplasts and Western blotting and MALDI-TOF of nuclear extracts show that Avp

95 on of carbon sources, enzyme assays, Western blotting and mass spectrometric analysis to monitor and

96 Western blotting and molecular docking studies suggested that th

97 Western blotting and MS results indicated that rHBeAg and HBeAg

98 Western blotting and quantitative PCR analysis of infected cells

99 Immunohistochemistry, Western blotting and quantitative RT-PCR analyses revealed that

100 Western blotting and Real-time PCR were used to assess the expre

101 Tumor signaling was studied by Western blotting and reverse-phase protein array analysis.

102 ciferase assays, immunofluorescence, western blotting and virus infection.

103 gingival tissues were examined with Western blotting and zymography, respectively.

104 caused cell cycle arrest as shown in Western blottings and flow cytometry assays utilizing both Mino

105 LSE samples (quantitative RT-PCR and Western blotting) and in normal and AE skin biopsy specimens (im

106 ion of SacAcsA enzyme was studied by Western blotting, and acetylation of lysine residues Lys(237), L

107 iter plates, by two-dimensional (2D) Western blotting, and by surface plasmon resonance demonstrated

108 gation, immunoprecipitation, dot and Western blotting, and confocal imaging were performed for signal

109 lls was monitored by flow cytometry, Western blotting, and confocal/electron microscopy.

110 enomic RNA strands were detected by Northern blotting, and crystalline lattices of viral particles of

111 ntitative polymerase chain reaction, Western blotting, and DNA binding assays (for transcription fact

112 qRT-PCR, Western blotting, and enzymatic assays were performed to confirm

113 ein sialoforms by mass spectrometry, Western blotting, and flow cytometry.

114 ve electron microscopic morphometry, Western blotting, and functional tests.

115 mined by quantitative real-time PCR, Western blotting, and immunofluorescence in human alveolar epith

116 paracellular flux, mRNA expression, Western blotting, and immunofluorescence staining.

117 on, as examined by real-time RT-PCR, Western blotting, and immunofluorescence.

118 ntitative reverse transcriptase-PCR, western blotting, and immunohistochemistry in CTCL cell lines an

119 Polymerase chain reaction, Western blotting, and immunohistochemistry were used to examine

120 evaluated by means of real-time PCR, Western blotting, and immunohistochemistry.

121 analyzed by using quantitative PCR, Western blotting, and immunohistochemistry.

122 1 CT was evaluated by real-time PCR, Western blotting, and immunohistochemistry.

123 by fluoroenzyme immunoassay, immuno-Western blotting, and line-blot immunoassay.

124 ocessed for histological evaluation, Western blotting, and metabolomics analyses using UPLC-QTOF/HDMS

125 re sampled for immunohistochemistry, western blotting, and mitochondrial high-resolution respirometry

126 acterization was performed with TEM, Western blotting, and NanoSight analysis.

127 cells were evaluated by using ELISA, Western blotting, and peptide epitope mapping.

128 p RNA sequencing, quantitative PCR, Northern blotting, and rapid amplification of cDNA ends methods.

129 e assessed using immunofluorescence, Western blotting, and reverse transcriptase-polymerase chain rea

130 s were identified by immunostaining, Western blotting, and RNA analysis.

131 ar pathways was assessed by means of Western blotting, and the final outcome on immunomodulatory prop

132 By far-Western blotting, and verified by coimmunoprecipitation, we obse

133 scription polymerase chain reaction, Western blotting, and zymography.

134 o-specific mass spectrometry; 2) far-Western blotting; and 3) live cell single-molecule imaging of SH

135 al perfusion, histological analysis, Western blotting, as well as transmission and scanning electron

136 In addition, H&E staining, TUNEL and Western blotting assays were performed in order to observed the

137 coming by demonstrating multiplexed northern blotting based on the mechanism of hybridization chain r

138 Western blotting, chromatin immunoprecipitation (ChIP), and luci

139 roarray, quantitative real-time PCR, Western blotting, chromatin immunoprecipitation, and functional

140 ns and dorsal striatum of rats using western blotting, co-immunoprecipitation, and chromatin immunopr

141 Quantitative PCR (qRT-PCR) and Western blotting confirmed changes in expression of seven of the

142 m for TEM images), and most importantly, dot blotting confirmed immunological activity of the collect

143 coactivator of ACTA2 Two-dimensional Western blotting confirmed induction of both wild-type and mutan

144 verse transcription-PCR (RT-PCR) and Western blotting confirmed that genes involved in DR-induced apo

145 Western blotting confirmed the enhanced protein levels of cytoso

146 ymerase chain reaction analysis, and Western blotting confirmed the presence of apical NaPi-2b and Nc

147 in mouse incisor enamel organs, and Western blotting confirmed their translation into protein.

148 lymerase chain reaction (RT-PCR) and Western blotting, confirming a concentration-dependent increase

149 erior affinity reagents suitable for western blotting, confocal fluorescence microscopy and pull-down

150 Western blotting consistently detected 50% BSE within a mixture,

151 Size exclusion chromatography and Western blotting data obtained by using purified recombinant E1/

152 olecular screening using qRT-PCR and Western blotting demonstrated that aldosterone treatment for 24

153 Cell fractionation and Western blotting demonstrated that beta-catenin fragments were t

154 Western blotting demonstrated that chronic hyperglycemia-associa

155 Western blotting (Dot Blot) as an immunological method confirmed

156 When using northern blotting during these studies, we discovered that miRNA-

157 In the blotting-EGTA model, exsA mutants were defective in capa

158 We used qRT-PCR, Western blotting, ELISA, and ChIP (chromatin immunoprecipitation

159 lded CWD prion protein (PrP(CWD)) by western blotting, ELISA, or immunohistochemistry (IHC).

160 APP using co-immunoprecipitation and Western blotting/ELISAs, respectively.

161 Northern blotting established that HCV dsRNA contained genome-len

162 ion and mRNA levels were analyzed by Western blotting, flow cytometry, and real-time PCR.

163 on of CHIKV protein expression using western blotting for CHIKV nsP1, nsP3, and E2E1 proteins.

164 Northern blotting for individual miRNAs showed that the proportio

165 a SMAD-luciferase reporter assay and Western blotting for phospho-SMAD2/3 and ZEB-2 in cultures of hu

166 3b-II/LC3b-I ratio, were detected by Western blotting from whole retinal extracts.

167 and traffic by confocal microscopy, western blotting, gel filtration, and immunoprecipitation studie

168 sulin signaling protein abundance by Western blotting, glucose tolerance and utilization, and insulin

169 ssay (VOPBA) in combination with far-Western blotting, gradient-purified EAV particles were shown to

170 In the present study, Western blotting identified a band corresponding to C3 in freshl

171 Phospho-arrays/Western blotting identified signalling activation in endothelial

172 lyp tissue were analyzed by means of Western blotting, immunoassays, or histology.

173 Studies by using dot blotting, immunoblotting, circular dichroism spectroscop

174 uced autophagosomes was confirmed by Western blotting, immunofluorescence microscopy, and immunoelect

175 ere validated by immunoprecipitation/Western blotting, immunofluorescence, and proximity ligation ass

176 Using western blotting, immunofluorescence, ELISA and qRT-PCR, we inve

177 established and then validated using western blotting, immunohistochemistry, and comparable TCGA RPPA

178 Using molecular cloning, RT-PCR, Western blotting, immunolocalization and in vitro functional ass

179 Western blotting, immunoprecipitation, and protein inhibition as

180 eaved) C3 fragments were detected by Western blotting in extracts of IL-1alpha-stimulated cartilage.

181 nerated and verified using q-PCR and western blotting in human keratinocytes (HaCaT) and endothelial

182 not glycoprotein B, was detected by Western blotting in isolated axons during Us9-null PRV infection

183 Western blotting in nelfinavir and its analog treated cells conf

184 ochemistry on kidney sections and by Western blotting in plasma samples from rats subjected to renal

185 Screen hits were validated with western blotting in the HDLM-2 Hodgkin's lymphoma cell line.

186 strate their superior performance in Western blotting, in both peroxidase- and fluorophore-linked for

187 chain reaction, flow cytometry, and Western blotting-in several nonprostatic cell lines and xenograf

188 or partners by mass spectrometry and western blotting, including DDX6, UNR, unrip, PAT1B, LSM14A and

189 A-NO preconditioning was observed by Western blotting, including elevated phosphorylation of NRF2, NF

190 Western blotting indicated that NMII-induced B1a cell death was

191 ted cells by electron microscopy and Western blotting indicated that PI3K-C2A was not required for th

192 Western blotting is a commonly used protein assay that combines

193 surface glycosylation, and for tissue paper blotting is likely due to cell permeabilization.

194 Western blotting is most often used for protein identification o

195 This improved northern blotting is particularly useful to analyze products of R

196 lysosome compartment evaluation, and western blotting light chain 3 (microtubule-associated protein 1

197 tion by quantitative Western and far-Western blotting, mass spectrometry, and computational modeling.

198 410 649 mum(2); P < .001) stains and western blotting (median, 2.01 vs 0.87; P = .009) demonstrated a

199 Here we present a modified Western blotting method that allows the rapid and reproducible q

200 ntitative real time PCR (n = 21) and western blotting (n = 9).

201 Western blotting of cross-linked SERCA revealed higher-molecular

202 Western blotting of MKK2 protein and its T31 phosphorylated pept

203 Western blotting of patient fibroblast and muscle showed decreas

204 members of the family as measured by Western blotting of platelet lysates.

205 To address this question, we used Western blotting of postmortem tissue from human V1 (12 female,

206 Western blotting of rodent retina and brain homogenates showed a

207 t complement RNA-seq, discover that northern blotting of small RNAs is biased against short sequences

208 Using Western blotting of STN tissue punches, we demonstrated that Glu

209 ependent MGMT silencing confirmed by Western blotting of tumor tissue ex vivo.

210 sed G-actin levels, as determined by Western blotting of vessel lysates, supporting involvement of cy

211 is protocol describes how to perform western blotting on individual cells to measure cell-to-cell var

212 Western-blotting on mouse cervix confirmed large scale histone d

213 SSA-52-kD subtype detected by immuno-Western blotting only.

214 erm storage methods (freezing, lyophilising, blotting onto filter paper); and freeze-thaw cycles.

215 or spleen of these animals by either Western blotting or after amplification by serial PMCAb.

216 chemical processes were evaluated by Western blotting or immunofluorescence.

217 tative label-free mass spectrometry, Western blotting, or confocal imaging.

218 in expression as measured by RT-PCR, Western blotting, or Luminex technology.

219 NA knockdown and ectopic expression, western blotting, PCR, promoter-luciferase reporter assays, chro

220 unchanged during superinduction, yet Western blotting, pulse labeling, and the use of bicistronic vec

221 re validated respectively by MRM and western blotting quantitative analyses.

222 ort of human brain tissue samples by Western blotting, quantitative PCR, and immunohistochemistry.

223 ed in detection of miRNAs including northern blotting, quantitative real time PCR (qRT-PCR) and micro

224 in hours, and it can be performed on western blotting quantities of endogenously ubiquitinated protei

225 l outcomes were assessed by means of Western blotting, real-time PCR, differentiation, and proliferat

226 le analysis, morphologic assessment, Western blotting, receptor binding, gene expression, small inter

227 Tissue paper blotting removed GalNAz-labeled surface cells, causing D

228 in levels were measured by ELISA and Western blotting, respectively, in blood samples from 101 patien

229 etected with quantitative RT-PCR and Western blotting, respectively, in both platelets and Meg-01 cel

230 Immunofluorescence microscopy and Western blotting results showed that RSV infection of human airw

231 Immunofluorescence microscopy and western blotting revealed marked disorganization and reduced COL

232 IHC and western blotting revealed reduction in epidermal hyperplasia (Ki

233 Western blotting revealed the presence of Pph (Ptb33) and Tph (T

234 with real-time quantitative PCR and Western blotting revealed up-regulation of C3 and CFB expression

235 s, supplemented by real-time PCR and Western blotting, revealed that the expression of critical regul

236 a molecular sieving matrix during PAGE and a blotting scaffold during immunoprobing.

237 tional western blotting, single-cell western blotting (scWB) is particularly useful for protein targe

238 RT-quantitative PCR and Western blotting showed reduced levels of enzymes catalyzing hom

239 through immunocytochemistry, whereas Western blotting showed that DDAH1 remained in the kidney and li

240 Western blotting showed that endogenous HMW PDE3A1 was the princ

241 Western blotting showed that oxytocin treatment blocked stress-i

242 n luciferase reporter gene assay and Western blotting showed that recombinant Tat or Tat-containing c

243 Far-Northwestern blotting showed that STMV CP promotes binding between HV

244 Western blotting showed that these channels also formed predomin

245 Immunofluorescence staining and Western blotting showed the presence of UGT1A in the basal and l

246 and extended by a 5'-RACE assay and Northern blotting, showing that meiotic cells induce the long iso

247 Like conventional western blotting, single-cell western blotting (scWB) is particu

248 uencing, quantitative real-time PCR, Western blotting, small interfering RNA interference, and kinase

249 and the severe loss of calpain 3 on western blotting, suggest a dominant negative effect with a loss

250 Western blotting suggested beta-conglutins were the main protein

251 ream regulator analysis coupled with Western blotting suggests that abnormal basal activation of the

252 heir sequence independence was determined by blotting synthetic peptide arrays, and they have been te

253 A triplex Western blotting technique was used to estimate the two allotype

254 assays based on combined microplate/Western blotting techniques that specifically detect either C3bB

255 Using mass spectrometry and Western blotting techniques, we have identified choline transpor

256 To conduct the Western Blotting test, recombinant MAF-1 fusion protein was used

257 ciprocally reactive with antisera by Western blotting, this truncated protein did not react with hMPV

258 for resolution while keeping separation and blotting times to less than 8 min.

259 from an "enclosed" microchannel for PAGE and blotting to an "open" PA gel lane for immunoprobing and

260 Here we use immunospot blotting to examine the efficiency of photoproduct forma

261 We used Western blotting to examine the phosphorylation of Stat1 and Sta

262 tative polymerase chain reaction and Western blotting to investigate changes in ion channel expressio

263 Here, we use quantitative Western blotting to show that the PAS domain is not required for

264 ted through gel electrophoresis with Western blotting, transmission electron microscopy, UV-Vis spect

265 the protein acetylation in cells by Western blotting (tubulin vs histone acetylation).

266 igomers, whereas immunoprecipitation-Western blotting using high-detergent extracts revealed a variet

267 cipitation and simultaneous assay by Western blotting using multiplex fluorescence imaging with speci

268 Northern blotting using probes specific to hrcA, igr66 or dnaK re

269 These were further validated by Western blotting using specific Abs.

270 pectrometry, and further verified by Western blotting using specific Abs.

271 d as beta-lactoglobulin (beta-LG) by Western blotting using specific monoclonal antibody and patient'

272 Western blotting was performed to detect endogenous and overexpr

273 At the same time Western blotting was performed with the patient's serum of previ

274 transfection with miR-146b mimic and western blotting was used to analyse SOX5.

275 Western blotting was used to determine levels of IKKbeta express

276 e determined by neutral comet assay; western blotting was used to evaluate protein changes; changes i

277 J protein expression was assessed by Western blotting (WB) and immunocytochemistry (ICC).

278 Western blotting (WB) indicated that PrP(263K), PrP(CWD), and Pr

279 Western blotting (WB) was employed to validate the downstream ta

280 ssociated prion protein (PrP(Sc)) by Western blotting (WB), antigen capture enzyme immunoassay (EIA),

281 urther studied using bioinformatics, Western blotting (WB), immunocytochemistry, and coimmunoprecipit

282 (TDC) has been successfully used in Western blotting (WB).

283 n immunochemical techniques, such as western blotting (WB).

284 ed VEGF receptor 2 (pVEGFR2), and by western blotting we found that membrane VEGFR1 and VEGFR2 increa

285 Based on lipid analysis and Western blotting, we show that the bacteria-like entities consis

286 ceptor (GHRH-R) measured by qPCR and Western blotting were significantly down-regulated in retinas of

287 Microscopy and Western blotting were used to determine activation of signal tra

288 l-time polymerase chain reaction and Western blotting were used to evaluate Renal Klotho expression.

289 ave analyzed four commercial pAbs by Western blotting, which revealed prominent cross-reactivity with

290 reaction, immunohistochemistry, and Western blotting, while matriptase activity was monitored by flu

291 Northern blotting with comX probes revealed two distinct transcri

292 In addition, Western blotting with convalescent rabbit serum detected cell wa

293 superficial epithelial injury (tissue paper blotting with EGTA treatment) and immunocompromise (MyD8

294 We performed western blotting with enokitake extract and the patient's serum.

295 We performed Western blotting with enokitake extracts and the patient's serum

296 s as judged by mass spectrometry and Western blotting with phospho-specific antibodies.

297 frontal cortex and cerebellum using Western blotting with specific antibodies.

298 nse gene 88), or after superficial injury by blotting with tissue paper.

299 This assignment was confirmed by Western blotting with whole-tooth crown and enamel extracts.

300 (n = 20)) by automated quantitative western blotting, with excellent agreement with our proteomics f