ライフサイエンスコーパス: blunt end

1 ng restriction endonuclease that generates a blunt end.

2 unction with 25-bp arms, each of which had a blunt end.

3 ate, whereas the WRN exonuclease acts at the blunt end.

4 e across the surface, they move toward their blunt ends.

5 dded dNTPs reduced all 3' or 5' overhangs to blunt ends.

6 enerate 3' overhangs and then processed into blunt ends.

7 staggered ends rather than generally assumed blunt ends.

8 ard arrowCTG sequences as indicated, leaving blunt ends.

9 that can allow for fill-in DNA synthesis, to blunt ends.

10 aks with single-base 3' overhangs as well as blunt ends.

11 aves RGCY sites between the G and C to leave blunt ends.

12 AMPs), such as double-strandedness and dsRNA blunt ends.

13 teracts with the dsRNA backbone and not with blunt ends.

14 dian size of deletions when joining DNA with blunt ends.

15 slowly trims 3'-phosphoglycolate-terminated blunt ends.

16 d for efficient end joining, particularly of blunt ends.

17 in which each 'arm' of the 4-way junction is blunt-ended.

18 f of the chromosomes in flowering plants are blunt-ended.

19 We show that RIG-I's selectivity for blunt-ended 5'-ppp dsRNAs is approximately 3000 times hi

20 were used to construct duplexes with either blunt ends, 5'-overhangs, 3'-overhangs, a flap or a fork

21 the structure of these complexes formed with blunt-ended, 5'-extended, and 3'-extended DNA.

22 In contrast, the enzyme strand-separated blunt-ended, 5'-tailed, and 3'-tailed duplex DNA equally

23 Surprisingly, poliota's ability to blunt-end a 1 bp recessed terminus is dependent upon the

24 The Klenow fragment promotes blunt end addition of dAMP; this reaction was much less

25 d over dNTPs as substrates for non-templated blunt-end addition, which typically entails the incorpor

26 crystallography to establish a mechanism for blunt-end additions catalyzed by Sulfolobus solfataricus

27 tacking pattern can be applied to subsequent blunt-end additions only if all incorporated dAMPs are e

28 Here we show that blunt-ended alpha-helical barrels, that is, preassembled

29 mpanied by a drastic loss of fidelity during blunt end and 5' overhang DSB repair.

30 Unwinding of the blunt end and 5'-overhang substrates appeared to initiat

31 exonuclease function is 2.5-7-fold higher on blunt end and 5'-recessed double-stranded DNA substrates

32 For substrates with a blunt end and a 3' overhanging end, Ku, XRCC4/Ligase IV,

33 cy, but addition of Ku suppresses joining of blunt ends and homologous ends with 3' overhangs.

34 The DNA fragments were repaired to create blunt ends and ligated to a new BAC vector.

35 labeled with (1) the TUNEL assay to identify blunt-ended and 3' protruding termini of breaks in doubl

36 hip-like, tapered tail tip, the male tail is blunt-ended and round.

37 required for RecBCD to initiate unwinding of blunt-ended and twin (dT)(6)-tailed DNA reflect processe

38 0-nucleotide 5'-overhang, a 50-bp dsDNA with blunt ends, and a Holliday junction with 25-bp arms, eac

39 are characterized by short microhomologies, blunt ends, and short insertions.

40 emplated nucleotides to primer-templates and blunt ends, and their preference for rNTPs versus dNTPs.

41 roviral DNA made by reverse transcription is blunt-ended, and the viral integrase protein must remove

42 Linear dsDNAs with recessed complementary or blunt ends are not utilized.

43 Thus, although single-base 3' overhangs and blunt ends are present in apoptotic nuclei, the specific

44 Extracts depleted of Ku and DNA-PKcs rejoin blunt ends, as well as homologous ends with 3' or 5' pro

45 ctly in the crystals, even though the DNA is blunt-ended at the active site.

46 Several DNA donors containing either normal blunt-ended att sites or different end mutations did not

47 phosphate (ppp) moiety in conjunction with a blunt-ended, base-paired region at the 5'-end (reviewed

48 Blunt-ended breaks bearing 5' phosphates were detected i

49 Recessing the DNA from a blunt end by even one nucleotide caused the RT to reorie

50 igation between injected molecules that have blunt ends can also occur within the germ line.

51 but, surprisingly, only when 5' overhangs or blunt ends can be generated.

52 , and the Ku-dependence of Artemis action at blunt ends can be reconciled with the other nucleolytic

53 Once the trocar is removed, the blunt-ended cannula can be advanced or can be subject to

54 hemical steps in Tn5 transposition result in blunt end cleavage of the transposon from the donor DNA.

55 Our in vivo blunt-end cloning method and destabilization-domain-fuse

56 Dynamic blunt end contacts promote error correction and network

57 leolytic" families branched out, separating "blunt end cutters" from "5' four-base overhand cutters."

58 Our kinetic studies indicated that the first blunt-end dATP incorporation was 80-fold more efficient

59 Although a blunt-ended derivative of the pre-miR-30 stem-loop remai

60 hes the fluorescence less efficiently than a blunt end dG-dC or dC-dG base pair.

61 Ends with 3' extensions or blunt ends did not show enhanced frequencies of errors,

62 In contrast, we show that blunt-ended dimers are able to form nanofibers with a un

63 foster fibrillogenesisthat is, as opposed to blunt-ended discrete speciesthe terminal quarters of the

64 binding modules are required for ligation of blunt ended DNA substrates.

65 functions in vivo and in vitro by incubating blunt-end DNA constructs having vertebrate telomeric end

66 hich vaccinia virus topoisomerase bound to a blunt-end DNA joins the covalently held strand to a 5' r

67 s to target Srs2, under optimized conditions blunt-end DNA substrates are also dissociated by this pr

68 A 2.05 A resolution structure of Dpo4*(blunt-end DNA)*ddATP revealed that the base and sugar of

69 ere found to bind to the surface-immobilized blunt-end DNA, and translocate along the DNA substrates

70 mplate-independent nucleotide additions onto blunt-end DNA.

71 d to describe the ATP-dependent unwinding of blunt-ended DNA by RecBCD in vitro is a sequential n-ste

72 ggested that it might be a useful enzyme for blunt-ended DNA cloning.

73 roximately 6 bp upon binding to the end of a blunt-ended DNA duplex in a Mg(2+)-dependent but ATP-ind

74 ave found that the Tte-UvrD helicase unwinds blunt-ended DNA duplexes as well as substrates possessin

75 nded DNA overhang but not of 5' overhangs or blunt-ended DNA fragments.

76 leaves that target at its center to generate blunt-ended DNA fragments.

77 mia virus reverse transcriptase complexed to blunt-ended DNA in three distinct lattices.

78 In contrast, we find that blunt-ended DNA is a better substrate for the biological

79 Both cohesive- and blunt-ended DNA molecules were end-joined, although the

80 corporated approximately 20-25% of the input blunt-ended DNA substrate into the stabilized ISD comple

81 f-site integration products are reduced with blunt-ended DNA substrate that must first be processed b

82 ease activities, and unwinds and digests the blunt-ended DNA until a specific eight-nucleotide sequen

83 Interestingly, RecQ[Bs] unwinds blunt-ended DNA with structural features, including nick

84 s the exonuclease activity of Artemis toward blunt-ended DNA, and promotes slow and limited endonucle

85 It had little or no activity on blunt-ended DNA, DNA with a 3' protruding strand, or sin

86 With blunt-ended DNA, Ku protected against Exo1 in a manner t

87 As with blunt-ended DNA, RecBCD unwinding of DNA possessing 3'-(

88 n by increased ionic strength than repair of blunt-ended DNA.

89 on for bleomycin-induced DSBs as compared to blunt-ended DNA.

90 -stranded 3'-extension efficiently generates blunt-ended DNA.

91 eferentially cleaved 3'-overhangs and RNA in blunt-ended DNA/RNA duplexes.

92 have found that the helicase does not unwind blunt-ended DNAs or substrates with 3'-ss tails.

93 se activity results in increased fidelity of blunt-end double-strand break repair in vivo by virtue o

94 (ss-DNA), primer-template DNA (pt-DNA), and blunt-end double-stranded DNA (ds-DNA) show that the bin

95 cleaves at its restriction site, yielding a blunt-ended double strand, which is then digested by exo

96 e DNA modifications by generating RNA-guided blunt-ended double-strand breaks.

97 RIG-I is activated by blunt-ended double-stranded (ds)RNA with or without a 5'

98 ited state in the cytoplasm and activated by blunt-ended double-stranded (ds)RNAs carrying a 5' triph

99 ds weakly to single-stranded DNA (ssDNA) and blunt-ended double-stranded DNA (dsDNA) but strongly to

100 The identification of blunt-ended double-stranded DNA breaks at the embedded h

101 It does not bind to blunt-ended double-stranded DNA.

102 igate short, complementary overhangs but not blunt-ended double-stranded DNA.

103 Total RNA was extracted and converted to blunt-ended, double-stranded cDNA by oligo(dT)-mediated

104 However, if a blunt-ended ds oligonucleotide contained the 22-bp Rep b

105 vation, no helicase activity was detected on blunt-ended ds oligonucleotide substrates unless they al

106 plexes with a 3'-overhang but is inactive on blunt-ended dsDNA and 5'-overhung duplexes.

107 PB unwinds dsDNA with 3' extensions, but not blunt-ended dsDNA, unless it contains a lesion, as shown

108 residues are involved in the recognition of blunt-ended dsRNA and 5' ppp dsRNA.

109 Here, we show that RIG-I CTD binds blunt-ended dsRNA in a different orientation compared to

110 acentrifugation demonstrated that LGP2 binds blunt-ended dsRNA of different lengths, forming complexe

111 y for 5' triphosphate (ppp) dsRNA as well as blunt-ended dsRNA.

112 is approximately 3000 times higher than non-blunt ended dsRNAs commonly found in cellular RNAs.

113 interface as a barrier to select against non-blunt ended dsRNAs.

114 stronger than the corresponding 36-mer with blunt ends due to slower dissociation.

115 Instead of forming a blunt end duplex with two a+.c mispairs and six Watson-C

116 mplate DNA but not to single-stranded DNA or blunt-end duplex DNA.

117 Instead of the expected blunt-end duplex with four consecutive G x U pairs, the

118 owever, NS3 was not able to rapidly unwind a blunt-end duplex.

119 e of nucleocapsid protein, and nature of the blunt-ended duplex (DNA/DNA versus RNA/DNA) had only lim

120 er, both enzymes efficiently unwind the same blunt-ended duplex containing a centrally located 12 nt

121 o-crystal structure of Taq polymerase with a blunt-ended duplex DNA bound to the polymerase active-si

122 t out approximately 5-6 bp upon binding to a blunt-ended duplex DNA in a Mg(2+)-dependent, but ATP-in

123 A blunt-ended duplex DNA primer was not utilized by telome

124 : (i) non-templated nucleotide addition to a blunt-ended duplex DNA primer; (ii) non-templated additi

125 s) catalyse the exonucleolytic hydrolysis of blunt-ended duplex DNA substrates and the endonucleolyti

126 winds a variety of DNA substrates, including blunt-ended duplex DNA.

127 y adenylated LigIII that positions a pair of blunt-ended duplex DNAs for efficient and specific inter

128 e ternary complexes were not observed with a blunt-ended duplex substrate.

129 dp1 has a preference for single-stranded and blunt-ended duplex substrates over nicked and tailed dup

130 for the incoming dNTP with an RT bound to a blunt-ended duplex was at least 1000-fold higher than wi

131 ot significantly digest single-stranded DNA, blunt-ended duplex, or a protruding strand of a partial

132 and five DNA substrates (long forked duplex, blunt-ended duplex, single-stranded DNA, 5'-overhang dup

133 ffected by the sequence at the 3' end of the blunt-ended duplex.

134 angs relative to the 3' overhangs but not to blunt-ended duplex.

135 of efficient addition of nucleotides to the blunt-ended duplex; this activity was also apparent in t

136 Binding of T5 exonuclease to either blunt-ended duplexes or single-stranded oligonucleotides

137 Interestingly, eIF4A is able to unwind blunt-ended duplexes.

138 nable to inhibit activation of RLRs by dsRNA blunt ends (end capping).

139 is similar to that observed for nontemplated blunt-end extension, suggesting that stacking interactio

140 The final joining of these blunt ends followed the same kinetics as signal joint fo

141 en the fourth and fifth nucleotide to give a blunt-ended fragment.

142 as evaluated by detection of DNA damage with blunt end fragments, whereas apoptosis was assessed by t

143 nts containing 5' or 3' overhangs but not of blunt-ended fragments.

144 added to the 5' end of the primer to form a blunt-end hairpin when the primer is not incorporated in

145 ix peptides that form three target parallel, blunted-ended heterodimers in preference to any of the o

146 OH processing, and the DNA was predominantly blunt ended in the complex.

147 icted to leave short 3' overhangs as well as blunt ends in digestion of chromatin.

148 unwind substrates with 5' or 3' overhangs or blunt ends in vitro.

149 Moreover, joining of SmaI-generated blunt ends is generally imprecise in the yeast strain us

150 succeeded in separating this mechanism from blunt-end joining by the biochemical fractionation of ex

151 gation of a singly nicked DNA duplex but not blunt-end joining.

152 reference sequences and junction structure (blunt-ended junctions or junctions with insertions or mi

153 elomerase will extend the newly synthesized, blunt-ended leading strand.

154 interactions between short DNAs (sDNAs) with blunt ends, leading to the formation of 3D nematic (N) a

155 ng hydroxyl group to the 5' phosphate of the blunt end, leaving the other strand unjoined.

156 ase activity by X4-LIV and the efficiency of blunt-end ligation are determined by structural configur

157 d that the major mechanism of joining was by blunt-end ligation following removal or fill-in of the s

158 However, blunt-end ligation was markedly more efficient than both

159 Modification of the blunt ends of the double-stranded stems was found to be

160 he junction compared with binding one of the blunt ends of the four-way junction to initiate unwindin

161 rhang substrates appeared to initiate at the blunt ends of these substrates.

162 icient exonuclease activity directed at both blunt ends of this substrate, whereas no activity is obs

163 d remains stacked and sandwiched between the blunt-ends of two adjacent Z-DNA duplexes, while the ove

164 nverted to a hairpin end on coding DNA and a blunt end on the neighboring recombination signal sequen

165 g-range ordering of DNA crossover tiles with blunt ends on lipid bilayers is investigated using atomi

166 DNase I footprinting of linear DNA with one blunt end, one primer-template junction, and binding sit

167 DNAs, but exhibits no detectable activity on blunt end or single-stranded DNA.

168 Termini with 5' overhangs, blunt ends or 3' termini with non-telomeric sequences at

169 Bleomycin DSBs are composed of blunt ends or ends containing a single 5'-base overhang.

170 The enzyme generates blunt ends or ends with 1-base 5'-overhangs possessing 5

171 ATM is activated by DSBs with blunt ends or short single-stranded overhangs (SSOs).

172 ingle-stranded tail or a forked end, but not blunt-ended or 3'-tailed duplexes.

173 remains controversial whether Cas9 generates blunt-ended or staggered-ended breaks with overhangs in

174 RecJ cannot resect duplex DNA that is either blunt-ended or terminated with 3'-ssDNA; however, such D

175 helicase can initiate resection of DNA with blunt-ends or 3'-ssDNA overhangs by DNA unwinding.

176 models (DNA constructs containing a nick, a blunt end, or a 3' extension) was evaluated.

177 erences for phosphorylation of 5' overhangs, blunt ends, or recessed ends, none of them displays the

178 The ends of a dsDNA break can be blunt-ended, or they may possess either 5'- or 3'-single

179 and decelerate the aging process as well as blunt end organ damage from obesity.

180 ingle-stranded DNA, double-stranded DNA with blunt ends, partially double-stranded DNA containing for

181 econdary structure within the extension or a blunt-ended pri-miRNA hairpin blocked Drosha cleavage.

182 ied Euplotes telomerase has no activity with blunt-ended primers.

183 hat there are multiple pathways that process blunt ends prior to end-joining.

184 Subsequent in situ ligation with blunt-ended probes revealed no 3' overhangs in necrotic

185 leases (EcoRV and HincII) that also generate blunt-ended products, and to a structurally distinct enz

186 and one using Pfu polymerase, which produces blunt-ended products.

187 d incorporation of the linearized, repaired, blunt-ended, rearranged genes into a suitable plasmid pe

188 within this gap are substrates for Alu I, a blunt end restriction enzyme.

189 base 3'-overhang with 3'-DNA residues on the blunt end result in a duplex form which directs dicing t

190 structures of human pol beta complexed with blunt-ended segments of DNA show that, although the crys

191 cial case because the RNA-DNA hybrid forms a blunt end, shown previously to resist cleavage when test

192 and show that lymphocyte flow begins within blunt-ended sinuses.

193 l dysgenesis with an abrupt termination of a blunt-ending spinal cord.

194 rent principle: the geometric arrangement of blunt-end stacking interactions.

195 greatly facilitated the secondary cut on the blunt-ended substrate, suggesting that NC compensates fo

196 substrates for half-site reactions than are blunt-ended substrates, which require the removal of two

197 n translocation along DNA is able to protect blunt-ended telomeres but is deficient in DNA repair.

198 We have previously suggested that blunt-ended telomeres in Arabidopsis thaliana are protec

199 Plants possess unusual blunt-ended telomeres that are unable to form t-loops or

200 This suggests that Ku physically sequesters blunt-ended telomeres within its DNA binding channel, sh

201 Run-off transcripts on a blunt-ended template were initially extended by 2-11 nt

202 oss from abasic sites and as 3'-overhangs on blunt-ended templates.

203 efficiency recircularization of cohesive and blunt-end terminated linear plasmid DNAs following trans

204 of purines and the O2 of pyrimidines at the blunt-end terminus.

205 RN is capable of unwinding duplex DNA from a blunt-ended terminus or from an internal nick.

206 eptide and form canonical heterodimers with "blunt-ends" that could not associate longitudinally.

207 mpletes the assembly of short (0.45 microns) blunt-ended thick filaments.

208 hat generate a single-stranded 3' end from a blunt end, thus activating the end for telomerase extens

209 These results suggest that small blunt-ended tiles can be used as a platform to organize

210 "Blunt-ended" tiles do not have single-stranded complemen

211 IN juxtaposes two DNA blunt ends to form the synaptic complex, which is the in

212 n a model incorporating DNA end breathing of blunt ends to form transient single to double strand bou

213 iated with growth (sheetlike projections and blunt ends) to those associated with shortening (rams' h

214 g 3' terminus into an exonuclease III-active blunt end, triggering the digestion of the probe into mo

215 a downstream A-rich tract (ENE+A) to form a blunt-ended triple helix composed of nine U*A-U triples

216 3'-terminal A of the A-rich tract to form a blunt-ended triplex lacking unpaired nucleotides at the

217 DNA containing a 3' single-stranded end, or blunt end, unlike the Rep68 and Rep52 enzymes, which hav

218 electrophoresis, using recombinant IN with a blunt-ended viral DNA substrate, we identified the synap

219 etween two ends with 3'-PSS, and between two blunt ends was most Ku dependent.

220 e detection of double-strand DNA breaks with blunt ends was performed by in situ ligation.

221 3'-phosphoglycolate-terminated overhangs and blunt ends were also processed by Artemis but much more

222 x fragments were converted to fragments with blunt ends, whereas protected fragments retained their c

223 n which leading-strand synthesis generates a blunt end while lagging-strand synthesis produces a long

224 However, NHEJ of blunt ends, while very inefficient, is not further reduc

225 For a blunt end with either a 3'-phosphoglycolate or 3'-hydrox

226 e that most of the broken DNA molecules have blunt ends with 3'-hydroxyl groups.

227 risingly, exo1Delta pol4Delta mutants repair blunt ends with a very low frequency of deletions.