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5 The delivery of large DNA vectors (>100 000 bp) remains a limiting step in the engineering of mammal
7 s containing viruses with genomes of <40,000 bp, and 4 of 6 specimens positive for large-genome virus
9 sequences from California (mt-CApsy, 15,027 bp) and Florida (mt-FLpsy, 15,012 bp), USA, were acquir
10 s) had genome lengths of 178,262 bp, 171,045 bp, and 171,454 bp, respectively, and were phylogenetica
13 es of the landscape at a smaller scale (5-10 bp) could be recovered in favorable regions at the begin
16 fragment containing the five motifs (the 10-bp, 10-bp-like, 10-bp-related, Region 2 and Fifth motifs
17 islands are quite short ( approximately 100 bp), are enriched near active genes, and often occur at
18 re observed with small DNAs and RNAs (10-100 bp): high voltages and short run times produced sharper
20 A becomes fragmented and damaged (often <100 bp) and may persist in the environment for more than hal
23 iscovery, in particular for indels of 30-100 bp, the 'NGS twilight zone of indels', in the Genome of
24 mplify fragments (horse; 85 bp, soybean; 100 bp, sheep; 119 bp, poultry; 183 bp, pork; 212 bp and cow
25 However, for DNA fragments shorter than 100 bp, all sets of parameters performed poorly yielding res
26 SVs, especially in the range from 30 to 100 bp, which we call the next-generation sequencing (NGS) t
27 ied out with short (36-50 bps), long (75-100 bps), single-end, or paired-end reads, the impact of the
28 nt distances ( approximately 20, 50, and 100 bps), we analyzed the repair outcomes by deep sequencing
29 nd STAT1 sites located between -777 and -105 bp (region A) and -921 and -796 bp (region B), respectiv
31 east 8 bp or a DNA-RNA duplex of at least 11 bp; RNA-RNA duplexes were more effective than DNA-RNA.
32 s (horse; 85 bp, soybean; 100 bp, sheep; 119 bp, poultry; 183 bp, pork; 212 bp and cow; 271 bp) of th
33 ngth, and 10 dsRNA segments ranged from 1192 bp (S4) to 3958 bp (L1), encoding 12 viral proteins.
35 o different DNA regions in beef (106 and 120-bp) and buffalo (90 and 138-bp) mitochondrial genes to d
36 tal monitoring) and long amplicons (800-1200 bps, designed to cover the entire resistance genes).
41 CENP-A protects DNA lengths centered on 133 bp, consistent with octameric nucleosomes with DNA unwra
43 eef (106 and 120-bp) and buffalo (90 and 138-bp) mitochondrial genes to discriminate beef and buffalo
44 bp) and subnucleosome-sized (such as 101-140 bp) peaks of digested chromatin fragments with MPE-seq b
45 pulations we have sequenced a portion (8,141 bp) of the mitochondrial (mt) genome in 28 specimens of
46 dobrensis and L. bryoniae, which were 16,141 bp, 16,236 bp and 16,183 bp in length, respectively.
47 size, and thus the CyHV3 genome, at 295,146 bp, remains the largest recorded among the herpesviruses
48 lysis of the assembled draft genome (1462509 bp) revealed the presence of 29 putative reductive dehal
50 tively large minimum sequence lengths (>/=15 bp) compared with the average length of known transcript
51 nome sequencing using short-read (e.g., <150 bp), next-generation sequencing technologies has reinvig
53 When homology at the matched end is </=150 bp, efficient repair depends on the recombination enhanc
54 When homology at the second end is </=150 bp, second-end capture becomes inefficient and repair sh
56 osomal DNA sizes varying between 147 and 155 bp, with positions of the MNase cuts reflecting position
59 atosome, containing H1 and approximately 160 bp, and then converts it to a core particle, containing
60 ) within the TNFA promoter (at -163 and -161 bp) displayed increased methylation in CP samples compar
61 intermediate particles containing 154 or 161 bp, corresponding to 7 bp protruding from one or both si
62 onths (16 of 21 patients; mean increase, 175 bp [95% CI, 79 to 271]) and 12 months (16 of 18 patients
63 ence of croaker elovl4, which contained 1794 bp (excluding the polyA tail), including 909 bp of codin
64 ted p53 REs contain spacers between 1 and 18 bp; however, their functional significance is unclear at
65 soybean; 100 bp, sheep; 119 bp, poultry; 183 bp, pork; 212 bp and cow; 271 bp) of the mitochondrial c
67 r various PCR products consisting of 180-190 bp (dsDNA) were recorded by Osteryoung square-wave volta
68 requently observed nucleosome-sized (141-190 bp) and subnucleosome-sized (such as 101-140 bp) peaks o
71 ir has a unique 93 base pair (bp)-long (+/-2 bp) ArgR-binding sequence containing two ARG boxes (39 b
75 e show that fragmented DNA molecules (>/= 20 bp) that additionally may contain abasic sites, cross-li
76 npaired guanosines flanking short (12- to 20-bp) dsDNA (Y-form DNA) were highly stimulatory and speci
77 tory mechanisms presented include long (>200 bp) noncoding RNAs that function as an additional regula
80 ntain insertions from nearby DNA (within 200 bp) and can contain multiple donor sources--some distant
82 binding sites, while separated by nearly 200 bp, appear to communicate in order to provide full YqjI-
83 ith both short amplicons ( approximately 200 bps, representative of ARG amplicon lengths commonly use
84 metagenomes of varied read length (100-2000 bp) revealed that it correctly classified at least 5% mo
85 ites in long introns (especially those >2000 bp) have significantly lower pi (nucleotide diversity) a
86 MuGENT require large arms of homology (>2000 bp) surrounding each genome edit, which necessitates lab
94 ic amplification of yfiR (375 bp), hlyA (234 bp) and eaeA (151bp), being one of the primers for each
96 uencing data, EBNA3C sites coincided (+/-250 bp) with RUNX3 (64%), BATF (55%), ATF2 (51%), IRF4 (41%)
99 ruses (YSLPVs) had genome lengths of 178,262 bp, 171,045 bp, and 171,454 bp, respectively, and were p
100 , poultry; 183 bp, pork; 212 bp and cow; 271 bp) of the mitochondrial cyt b, lectin, 12S rRNA, 12S rR
104 the nuclease domains are too far apart (>30 bp) to dimerise and produce a double-strand DNA break us
105 trated Scalpel's power to detect long (>/=30 bp) transmitted events and enrichment for de novo likely
106 those of the GATK for indels shorter than 30 bp, achieving up to 90% precision overall, with >80% of
107 With read lengths of currently up to 2 x 300 bp, high throughput and low sequencing costs Illumina's
108 V1 and CyHV2 genomes are 291,144 and 290,304 bp, respectively, in size, and thus the CyHV3 genome, at
109 ove our highest-confidence resolution (6,305 bp) and an additional 111 SVs per genome below this reso
110 ntifies binding specificity over a large (31-bp) binding site by iteratively fitting a feature-based
111 equires a DNA-binding site separation of 310 bp, much longer than the length needed for natural loops
112 arcode primer pairs targeting short (127-314 bp) fragments of the cytochrome c oxidase I (CO1) DNA ba
113 ime-stamped second hypervariable region (330 bp) of G-gene sequence data (global, n = 483; and Ontari
115 tric GUS assays showed that the shorter (345 bp) and medium (827 bp) fragments of nsLTP promoter func
116 mitochondrial genome is comprised of 16,345 bp, and contains the expected 37 genes and control regio
118 2G results in two DNA fragments (418 and 350 bp) and of 23S rDNA A2143G results in three DNA fragment
119 different insertion sizes (approximately 350 bp, 600 bp, 3 kb, and 10 kb) of a Tennessee-accessed, gl
120 cing technologies produced reads of 25 or 36 bp, and only from a single-end of the library sequence.
122 variable regions 1 and 2 ( approximately 360 bp) with a de-noising pipeline that significantly improv
124 romoter or mutating AP-1 binding site at -37 bp (A>G) and -38 bp (C>T) reduces the TBP promoter activ
125 for the specific amplification of yfiR (375 bp), hlyA (234 bp) and eaeA (151bp), being one of the pr
128 s compared with baseline (mean increase, 386 bp [95% CI, 178 to 593]); in exploratory analyses, simil
133 an patients with small or no deletions (</=4 bp; 59 of 77 patients, 76.6%) in multivariate analysis (
134 f cognitive control was approximately 3 to 4 bps, demonstrating that cognitive control as a higher-le
135 ific cis-activation elements, termed Site 4 (bp -7997 to -7988) and Site 12 (bp -7835 to -7826).
136 Nucleotide sequence analysis revealed a 4-bp (AGTC) insertion in the oprD gene, resulting in a fra
137 , in part, to the small DNA fragments (</=40 bp) directly produced by high LET radiation, the size of
138 eferentially to DNA molecules longer than 40 bp, and two CAF1-H3-H4 complexes concertedly associate w
139 othesis, BoHV-1 DNA fragments (less than 400 bp) containing potential GR and KLF binding sites were i
143 oS transcription initiation sites located 43 bp (P1) and 63 bp (P2) upstream of the rpoS start codon.
145 ngths of 178,262 bp, 171,045 bp, and 171,454 bp, respectively, and were phylogenetically closely rela
146 vel enhancer, located between +4517 and 4662 bp, but the luciferase reporter assay demonstrated that
150 ation events at both short ( approximately 5 bp) and long ( approximately 1 kb) genomic distances, sh
152 ercoiling in fibers with L = 10n and 10n + 5 bp, different flexibility of the two types of fibers, an
153 DNA packaging step-size of approximately 2.5 bp, such that the step-size is probably determined by th
154 f nucleosomes separated by approximately 4/5 bp, placing nucleosomes on opposite faces of the DNA.
155 y and had large HSP110 T(17) deletions (>/=5 bp; 18 of 77 patients, 23.4%) had longer times of relaps
156 novel large insertions, small indels (10-50 bp), and short tandem repeat expansions and contractions
157 ttP and attB attachment sites are small (<50 bp), there are no host factor or DNA supercoiling requir
158 variants (SNVs) plus 0.7 million small (1-50 bp) insertions and deletions (indels) that are consisten
161 short average length of approximately 25-50 bp, which is significantly shorter than the length of CO
162 Once single-end reads are at a length of 50 bp, the results do not change substantially for any leve
163 eriments to be carried out with short (36-50 bps), long (75-100 bps), single-end, or paired-end reads
166 ng mitochondrial DNA (mtDNA) haplotypes (500 bp), microsatellite genotypes (17 loci) and sex from 128
167 in human cells at the 1-3 nucleosome (50-500 bp) scale, obtained using ionizing radiation-induced spa
169 ccumulation over the first approximately 500 bp, suggesting that Spt5 is required for transcription p
170 genomic DNA (gDNA) fragments of length >500 bp, and it can successfully discriminate single mismatch
171 n (2.7), and low linkage disequilibrium (500-bp) were observed in Chikhwawa District, Malawi, an area
172 f 31 BFU-E were heterozygous for CALR del 52 bp, and 1 of 31 BFU-E was homozygous for CALR del 52 bp.
173 -order repeat structure, named Nazca, of 528 bp, whereas CentPv2 is composed of tandemly organized mo
174 B virus (WSHBV) has a circular genome (3,542 bp) with the prototypical codon organization of hepadnav
175 e chromosome's relatively small size (16,569 bp) necessitates the ability to detect extremely focal e
183 s able to handle reads with both short (<600 bp) and long (>35 000 bp) insert sizes producing high-qu
184 ion involves step-like events of 200 nm (600 bp) size and is strongly suppressed by forces above 1 pN
185 t insertion sizes (approximately 350 bp, 600 bp, 3 kb, and 10 kb) of a Tennessee-accessed, glyphosate
186 ged on one long ribosomal RNA operon of 6000 bp) and clusters of such clusters (perhaps reflecting a
187 e and consists of a unique sequence (174,601 bp) flanked by a terminal direct repeat (2,910 bp).
188 nnealing linked with NHEJ, inserting 4 to 61 bp, mostly originating from the surrounding of breakpoin
190 n that it requires a minimal sequence of >65 bp; however, other than a GAGA motif, the three Fab-7 LB
191 anti-silencing properties for the small (679 bp) CBX3-UCO element and we now confirmed this observati
192 t to generate high-quality large-insert (680 bp) paired-end libraries using a range of 50 pg to 50 ng
193 is gene, the DNA sequence from -2612 to +682 bp (relative to the transcription start site) of the JDP
194 urth (YSLGV), with a genome length of 73,689 bp, was related to group III in the extended family Mimi
198 e used 344 mitochondrial control region (717 bp) sequences from the finless porpoise (genus Neophocae
199 etic enhanced green fluorescent protein (720 bp) clones from 0.93% to 83.22%, corresponding to a decr
200 cies, Diabrotica virgifera virgifera (16,747 bp) and Diabrotica barberi (16,632; Insecta: Coleoptera:
202 2, TL increased in the intervention arm (+76 bp) and decreased in the controls (-23 bp) (p=0.050).
203 777 and -105 bp (region A) and -921 and -796 bp (region B), respectively, as responsible for the high
204 fficient TALE-VP64s bound around -120 to -80 bp, while highly effective sgRNAs targeted from -147 to
205 one pair of inverted repeats (IRs) of 26,819 bp, which were separated by one small single copy (SSC;
206 ed that the shorter (345 bp) and medium (827 bp) fragments of nsLTP promoter function as grain-specif
207 onstructed HBV haplotypes in a region of 836 bp, which contains the major immune epitopes and drug re
208 ic primers that amplify fragments (horse; 85 bp, soybean; 100 bp, sheep; 119 bp, poultry; 183 bp, por
209 Unambiguously aligned sequences (13,872 bp) from those 16 species plus 78 percomorphs and two ou
210 s produced 16,115 contigs with an N50 of 889 bp, over 90% of which has significant sequence similarit
212 e duplex, with a 2.5-fold increase between 9 bp (k'on = 2.1(1) x 10(6) M(-1) s(-1)) and 6 bp (k'on =
213 , varying by nearly 600-fold over the same 9 bp (koff = 0.024 s(-1)) to 6 bp (koff = 14 s(-1)) range.
215 order of magnitude when positioned close (9 bp) to the promoter, in comparison to that for a distal
216 edian deletion size using single sgRNAs is 9 bp, we also obtain large deletions of up to 600 bp.
218 recognition sites are separated by up to 90 bp, Fis represses IHF binding and weak DnaA interactions
221 utions per site within the concatenated (945 bp) nucleotide dataset, implying that probabilistic phyl
222 SuRCA), with an N50 scaffold size of 464 955 bp (based on a genome size of 606 Mbp), 221 640 contigs
223 hs of TmELO1 and TmELO2 were 1005 bp and 972 bp, respectively and the corresponding peptide sequences
225 ously explained by an initial >457 basepair (bp) HSV-1 x HSV-2 crossover followed by back-recombinati
226 y Huguet et al., the reconstructed basepair (bp) free energies agree with the running average of the
227 large soil metagenomes totaling 398 billion bp (equivalent to 88,000 Escherichia coli genomes) from
228 ding stronger preferences for A*T versus G*C bps, TA versus GG steps, and also suggests enrichment at
229 ttery dating from about 15,000 to 11,800 cal bp (the Incipient Jomon period), the oldest pottery so f
230 12,000 calibrated years before present (cal bp), towards the end of the Late Pleistocene epoch, a pe
231 s in an increase of approximately 1 degrees /bp (or a superhelical density of Deltasigma approximatel
232 th an array of Shockley partial dislocations bp:-bp on every basal plane and the 30 degrees twist GB
233 als that in both cases, m1A forms a m1A*T HG bp, which is accompanied by local and global structural
234 ere, we use structural features unique to HG bps (syn purine base, HG hydrogen bonds and constricted
236 anscription, which are found within areas I (bp -2694 to -2561), II (bp -2139 to -1958), III (bp -187
237 ound within areas I (bp -2694 to -2561), II (bp -2139 to -1958), III (bp -1879 to -1799), and IV (bp
243 lilee, Israel) and dated to 54.7 +/- 5.5 kyr bp (arithmetic mean +/- 2 standard deviations) by uraniu
244 During the Last Glacial Maximum (25-15 kyr bp), diversity declined markedly, although forbs remaine
245 d radiocarbon years before present (cal. kyr bp), glacial retreat opened an approximately 1,500-km-lo
247 ence of moose and elk at about 11.5 cal. kyr bp, and boreal forest approximately 10 cal. kyr bp.
248 glaciated North America before 12.6 cal. kyr bp, are unlikely to have travelled by this route into th
249 n and mammoth by approximately 12.6 cal. kyr bp, followed by open forest, with evidence of moose and
250 the stabilization of low-boiling point (low-bp) perfluorocarbons (PFCs) at physiological temperature
252 Y chromosome from wild horse assemblies (3 M bp) and Mongolian horse (2 M bp) were also sequenced and
256 d dystrophy (adCORD) caused by 12 base pair (bp) deletion at proline 351 of hAIPL1 (P351Delta12) muta
257 rmed that cats homozygous for a 2 base pair (bp) deletion within IQ calmodulin-binding motif-containi
259 d complexed with two different 22-base pair (bp) double-stranded operator sequences (oligos AT and AA
261 associated with approximately 80 base pair (bp) of DNA, which is located at a position that correspo
262 compared the processing of a 460 base pair (bp) perfect palindrome in the Escherichia coli chromosom
263 to evaluate whether the HLA-G 14-base pair (bp) polymorphism (rs16375) has an impact on human immuno
264 sing optical tweezers with single base pair (bp) resolution, we analyzed DNA unwinding by XPD helicas
265 e, recognizes a palindromic eight base pair (bp) symmetric sequence, 5-ATTTAAAT-3, and cleaves that t
266 Each peak-pair has a unique 93 base pair (bp)-long (+/-2 bp) ArgR-binding sequence containing two
267 We found a rare noncoding 12-base-pair (bp) deletion (del12) in intron 4 of ASGR1, which encodes
268 xon 18 of BRCA1, we replace a six-base-pair (bp) genomic region with all possible hexamers, or the fu
271 nding within conserved Region 3 (base pairs (bp) -8118/-7750) of MafA and Area II (bp -2153/-1923) of
272 es short paired-end tags (2 x 20 base pairs (bp)) to detect two genomic loci that are far apart on li
273 N cDNA sequence consists of 4226 base pairs (bp), comprising a 522-bp 5' untranslated region (UTR), a
276 ble-stranded DNA (dsDNA) over 40 base pairs (bp) in length has been considered immunostimulatory.
277 pproximately 99% and length 6050 base pairs (bp) in the human genome, and 16 Tol2 elements of identit
278 protein (ZF6-DB) that targets 20 base pairs (bp) of a RHOcis-regulatory element (CRE) and demonstrate
279 nslocations have between 0 and 4 base pairs (bp) of microhomology (n = 26), short inserted sequences
281 encing short fragments of 50-100 base pairs (bp) that accumulate in long internucleosome linker regio
282 ly bell-shaped with a peak at 50 base pairs (bp) upstream of the transcription start site (TSS) and 8
286 double helix, Watson-Crick (WC) base pairs (bps) exist in dynamic equilibrium with sparsely populate
287 map since short reads of 50-100 base pairs (bps) from these regions map to multiple locations in ref
288 behind a footprint of up to five base pairs (bps) of the original VH gene that is often further obscu
290 Hg and/or a diastolic BP >/= 90 (HTN(doc,pat,bp)) and diagnosis according to the National Health and
294 te of cognitive control (in bits per second, bps) in the model fitting to estimate the capacity of co
295 and constricted C1'-C1' distance across the bp) to search for HG bps in X-ray structures of DNA dupl
296 -5-FX interaction with FX-binding protein (X-bp) inhibited transduction in the presence of C57BL/6 se
297 uman bones date to 10,705 +/- 35 (14)C years bp (approximately 12,707-12,556 calendar years bp) and w
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