ライフサイエンスコーパス: butyryl

1 Furthermore, butyryl ACP itself underwent slow conformational exchang

2 rate-bound form of ACP (crystal structure of butyryl-ACP from Escherichia coli) suggests that conform

3 Unexpectedly, butyryl act ACP showed almost identical backbone (1)H-(1

4 iled analyses, which indicated that (1) only butyryl-acyl carrier protein (ACP) and S-adenosylmethion

5 hway is based on a thioesterase specific for butyryl-acyl carrier protein (ACP), which allows native

6 tonization but did induce the deacylation of butyryl-acyl carrier protein.

7 yl-ADP-ribose, O-propionyl-ADP-ribose, and O-butyryl-ADP-ribose to produce ADP-ribose (ADPr) and acet

8 ost potent (EC(50): 1 nM), followed by the N-butyryl analogue.

9 oxidation cycle with optimal activity toward butyryl- and hexanoyl-CoA.

10 nhibitor (IC50 = 30 nM), 1-(2-amino-3-methyl-butyryl)-azetidine-2-carbonitrile (AMAC), which has show

11 similar analog, 1-(2-dimethylamino-3-methyl-butyryl)-azetidine-2-carbonitrile (DAMAC), that does not

12 talyzed the transfer of acetyl-, propionyl-, butyryl-, benzoyl-, phenylacetyl-, and malonylphosphopan

13 tion of T(alpha) peptides carrying maleimido-butyryl-biocytin by avidin-agarose chromatography; and (

14 N-maleimide moiety of the reagent, maleimido-butyryl-biocytin, containing a biotinyl group; (iv) tryp

15 4, 6, or 7 conferred both acetyl (AChE) and butyryl (BuChE) cholinesterase inhibitory activities at

16 cGMP and its analogs, 8-Br-cGMP and 2'-butyryl-cGMP, also competed with the Pgamma-1-83BC C ter

17 viously shown by proton NMR that horse serum butyryl cholinesterase, like serine proteases, forms a s

18 as monoamine oxidases (MAOs) and acetyl- and butyryl-cholinesterase (AChE and BChE) inhibitors.

19 In contrast, human or equine butyryl-cholinesterase (BuChE) converted CPT-11 to SN-38

20 e seen in mRNA levels of the related enzyme, butyryl-cholinesterase, nor of the high-affinity choline

21 e, acetoacetyl coenzyme A (acetoacetyl-CoA), butyryl CoA, acetoacetate, and beta-hydroxybutyrate.

22 talyze the condensation of malonyl-pfACP and butyryl-CoA (k(cat) 200 min(-1), K(M) 35.7 +/- 4.4 micro

23 imple equation: crotonyl-CoA + NADH + H(+) = butyryl-CoA + NAD(+) with Km = 1.4 mum ferredoxin or 2.0

24 Priming DEBS with endogenous butyryl-CoA affords an alternative and more direct route

25 the interconversion of isobutyryl-CoA and n-butyryl-CoA also catalyzes the interconversion between i

26 ible interconversion of isobutyryl-CoA and n-butyryl-CoA and exists as a heterotetramer.

27 nthesis in S. collinus, which suggested that butyryl-CoA and isobutyryl-CoA function as starter units

28 Butyryl-CoA and isobutyryl-CoA interacted with the acety

29 The DEBS polyketide synthase (PKS) used butyryl-CoA and methylmalonyl-CoA supplied in vivo by th

30 oA dehydrogenase has maximal activity toward butyryl-CoA and negligible activity toward substrates lo

31 ylate via a novel pathway thought to involve butyryl-CoA and propionyl-CoA as intermediates.

32 We have shown that we can produce butyryl-CoA at levels of up to 50% of the total CoA pool

33 tical to those seen for MM-CoA; in addition, butyryl-CoA binds and behaves in a manner similar to pro

34 e biosynthesis and that either acetyl-CoA or butyryl-CoA can be a starter unit for palmitate biosynth

35 substrates of ZhuH, although acetyl-CoA and butyryl-CoA could also be accepted and elongated.

36 dies, the biosynthesis of the palmitate from butyryl-CoA decreases in the presence of thiolactomycin.

37 Electron-transferring flavoprotein (Etf) and butyryl-CoA dehydrogenase (Bcd) from Acidaminococcus fer

38 A (CoA) dehydrogenase (BHBD), crotonase, and butyryl-CoA dehydrogenase (BCD) from Clostridium acetobu

39 ectron transferring flavoprotein (EtfAf) and butyryl-CoA dehydrogenase (BcdAf) of Acidaminococcus fer

40 ween an acetyl-, propionyl-, isobutyryl-, or butyryl-CoA derived primer unit and a malonyl-CoA derive

41 at CCR plays a significant role in providing butyryl-CoA for monensin A biosynthesis and is present i

42 reductase, which converts acetoacetyl-CoA to butyryl-CoA for use as a 4C extender unit during tylacto

43 tylicum are responsible for the formation of butyryl-CoA from acetoacetyl-CoA.

44 n high concentrations of 4-hydroxy-4-phospho-butyryl-CoA in brain and liver.

45 catalyzes the conversion of crotonyl-CoA to butyryl-CoA in the presence of NADPH, was previously clo

46 EBS 1+TE can convert acetyl-, propionyl-, or butyryl-CoA into the corresponding C8-, C9-, and C10-lac

47 particular, its tolerance toward acetyl- and butyryl-CoA is unexpectedly strong.

48 he high concentration of 4-hydroxy-4-phospho-butyryl-CoA may be related to the cerebral dysfunction o

49 e inactivation upon incubation with either n-butyryl-CoA or isobutyryl-CoA.

50 tase, a primary metabolic enzyme involved in butyryl-CoA production in streptomycetes, was expressed

51 lysines correlated with the acetyl-CoA: (iso)butyryl-CoA ratio in liver.

52 ed as a transcriptional unit and form a BCS (butyryl-CoA synthesis) operon.

53 The butyryl-CoA synthesized was further extended to hexanoyl

54 mutant using butyryl-, crotonyl-, and 2-aza-butyryl-CoA thioesters.

55 nd R207Q) to catalyze the rearrangement of n-butyryl-CoA to isobutyryl-CoA.

56 e the exergonic reduction of crotonyl-CoA to butyryl-CoA to the endergonic reduction of ferredoxin bo

57 lmitate (a branched-chain fatty acid), while butyryl-CoA was converted to palmitate (a straight-chain

58 in 824(pAADB1) fermentations suggested that butyryl-CoA was limiting butanol production in 824(pAADB

59 ed with GroEL/ES, and the R147W variant when butyryl-CoA was used as a substrate.

60 inactivation when either isobutyryl-CoA or n-butyryl-CoA was used as substrate.

61 f the loading didomain, although acetyl- and butyryl-CoA were also accepted with approximately 40-fol

62 t was unable to exchange the alpha-proton of butyryl-CoA with D(2)O.

63 ucial metabolic intermediates acetyl-CoA and butyryl-CoA with substantial velocities.

64 Optimal substrate (butyryl-CoA) was seen to shift the flavin redox potentia

65 9-h lag in growth was observed, during which butyryl-CoA, a degradation product of ethylmalonyl-CoA,

66 f 2.4 microM for acetyl-CoA, 0.71 microM for butyryl-CoA, and 0.41 microM for isobutyryl-CoA.

67 yme A (CoA) derivatives, such as acetyl-CoA, butyryl-CoA, HMG-CoA, and malonyl-CoA, as well as NADPH

68 substrate specificity and is able to accept butyryl-CoA, leading to the production of polyketides wi

69 th the results using our standard substrates butyryl-CoA, octanoyl-CoA, and palmitoyl-CoA.

70 the interconversion of isobutyryl-CoA and n-butyryl-CoA, whereas GTPase activity is associated with

71 ensation of two acetyl-CoA molecules to form butyryl-CoA, which is then transformed to succinyl-CoA w

72 h the priming of the enzyme by acetyl-CoA or butyryl-CoA.

73 ng acetyl-CoA approximately propionyl-CoA >> butyryl-CoA.

74 and Dh-FADH(-) that converts crotonyl-CoA to butyryl-CoA.

75 skeleton rearrangement of isobutyryl-CoA to butyryl-CoA.

76 enzyme caused the midpoint potential for the butyryl-CoA/crotonyl-CoA couple (E(BCoA/CCoA)) to shift

77 en measured in the presence and absence of a butyryl-CoA/crotonyl-CoA mixture.

78 Butyryl-CoA:acetate CoA-transferase activity was detecte

79 M), which interconverts isobutyryl-CoA and n-butyryl-CoA; ethylmalonyl-CoA mutase, which interconvert

80 Most of these genes were related to butyryl coenzyme A and butyrate formation and/or assimil

81 butyrate formation genes (ptb and buk); the butyryl coenzyme A biosynthesis operon genes; fructose b

82 ate kinase and phosphotransbutyrylase or via butyryl-coenzyme A (CoA):acetate CoA-transferase.

83 hen present at physiological concentrations, butyryl-coenzyme A and NADPH were not substrates for Rhl

84 of wild-type and the Glu367-Gln mutant using butyryl-, crotonyl-, and 2-aza-butyryl-CoA thioesters.

85 n from both cell types, as are forskolin, di-butyryl cyclic adenosine monophosphate, and adrenocortic

86 e of an acylated species of ACP is that of a butyryl derivative of Escherichia coli ACP.

87 f 7-deacetyl-7-(O-[N-methylpiperazino]-gamma-butyryl)-dihydrochloride-forskolin (MPB-forskolin), we f

88 nd 7-deacetyl-7-O-(N-methylpiperazino)-gamma-butyryl-forskolin was effective exclusively in the PFH,

89 n of 7beta-deacetyl-7beta-[gamma-(morpholino)butyryl]-forskolin hydrochloride (7Db-forskolin, a water

90 in when provided with a soluble beta-hydroxy-butyryl-heptapeptidyl thioester substrate.

91 Act ACP was also derivatised with butyryl, hexanoyl, and octanoyl groups.

92 ombinant wild type SCAD kcat/K(m) values for butyryl-hexanoyl-, and octanoyl-CoA were 220, 22, and 3.

93 (m) of 9.3, 2.8, and 1.5 microM-1 min-1 with butyryl-, hexanoyl-, and octanoyl-CoA used as substrates

94 inosa, along with its cognate autoinducer, N-butyryl homoserine lactone (C(4)-HSL), regulates gene ex

95 ne (3OC12-HSL) (formerly called PAI-1) and N-butyryl homoserine lactone (C4-HSL) (formerly called PAI

96 decanoyl)-homoserine lactone (C12-HSL) and N-butyryl homoserine lactone (C4-HSL), on cell viability a

97 production by a large excess of exogenous N-butyryl homoserine lactone (C4-HSL).

98 , and RhlI, which directs the synthesis of N-butyryl homoserine lactone (PAI-2).

99 Increased amounts of rhamnolipids and N-butyryl homoserine lactone were detected in the biofilm

100 system is composed of RhlR and the signal N-butyryl homoserine lactone.

101 N-(3-oxododecanoyl) homoserine lactone and N-butyryl homoserine lactone].

102 sI mutant PAO-MW1 alongside plasma treated N-butyryl-homoserine lactone and n-(3-oxo-dodecanoyl)-homo

103 , N-3-oxododecanoyl-homoserine lactone and N-butyryl-homoserine lactone, can both enter eukaryotic ce

104 ), whereas RhlI catalyzes the synthesis of N-butyryl-homoserine lactone.

105 2,3,6-Tri-O-butyryl-Ins(1,4,5)P(3)/AM (6-Ins(1,4,5)P(3)), a cell-per

106 l (k(cat)/K(m) 15.8 +/- 1.8 m(-1) s(-1)) and butyryl (k(cat)/K(m) 17.5 +/- 2.1 m(-1) s(-1)) derivativ

107 d exsC expression, resulting in increased N-(butyryl)-l-homoserine-lactone quorum sensing signal and

108 l-homoserine lactone (3-oxo-C(12)-HSL) and N-butyryl-l-homoserine lactone (C(4)-HSL), respectively.

109 (3-oxododecanoyl)-L-homoserine lactone and N-butyryl-L-homoserine lactone (C(4)-HSL), which are known

110 en coupled to FabI, purified P. aeruginosa N-butyryl-L-homoserine lactone (C4-HSL) synthase, RhlI, co

111 ; (4) RhlI was able to direct synthesis of N-butyryl-L-homoserine lactone from crotonyl-ACP in a reac

112 decanoyl)-L-homoserine lactone) and PAI-2 (N-butyryl-L-homoserine lactone) respectively.

113 N-(3-oxododecanoyl)-L-homoserine lactone, N-butyryl-L-homoserine lactone, and the Pseudomonas quinol

114 (3-oxododecanoyl)-L-homoserine lactone and N-butyryl-L-homoserine lactone.

115 convert authentic N-butyrylvinylglycine to N-butyryl-L-homoserine lactone.

116 heir cognate autoinducer ligand and not by N-butyryl-L-homoserine lactone.

117 onine (SAM) were required for synthesis of N-butyryl-L-homoserine lactone; (2) when present at physio

118 Interestingly, propionyl- and butyryl-lysine peptides were found to bind tighter to Hs

119 Sirt2, and Sirt3, suggesting propionyl- and butyryl-lysine proteins may be sirtuin substrates in viv

120 quinone chromophore in the dyad using an iso-butyryl mask.

121 d hyaluronan, the total NH2, N-acetyl, and N-butyryl moieties were 0, 82.2 +/- 4.6, and 22.7 +/- 3.8%

122 gly, NDTBT transferred hexanoyl, acetyl, and butyryl more rapidly than butenoyl or benzoyl from the C

123 ll permeant and non-radiolabeled 2,5,6-tri-O-butyryl-myo-inositol 1,3, 4-trisphosphate-hexakis(acetox

124 NANS module 2+TE with (+/-)-2-methyl-3-keto-butyryl-N-acetylcysteamine thioester (1), the SNAC analo

125 ine was found to be negligible and that of n-butyryl-pantetheinephosphate low, and therefore, it is e

126 The assay relies on 2'-amino-butyryl-pyrene-uridine incorporated in a 58-nucleotide r

127 A single intermediate, assigned to N-butyryl- S-adenosylmethionine, was observed.

128 the carboxylate oxygen of the presumptive N-butyryl-SAM intermediate attacks the methylene carbon ad