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1 minal computed tomographic scan was modified by inserting 10 simulated colonic polyps with use of met
2 ydrophobic core, we disrupted it in Galphai1 by inserting 4 alanine amino acids into the alpha5 helix
3 a new cell line as a molecular model of FRDA by inserting 560 GAA*TTC repeats into an intron of a GFP
4 wtIL-10 was converted to a monomeric isomer by inserting 6 amino acids into the loop connecting the
5 nerated a high diversity library of plasmids by inserting 90-base oligonucleotides of random sequence
6 urethral and bladder protection was provided by inserting a 12-F catheter infused with cold saline (8
10 tivation by the IAB5 enhancer can be delayed by inserting a 9.5 kb 3' Abd-B regulatory region contain
11 , we constructed an Ad-based malaria vaccine by inserting a B cell epitope derived from a Plasmodium
12 etaDelta protein can fill one nucleotide gap by inserting a base at the AP site, whereas a presumed b
13 on and expression, we made a Phr1 null mouse by inserting a beta-galactosidase/neor cassette into exo
14 log) showed that Rad4 "opens" up damaged DNA by inserting a beta-hairpin into the duplex and flipping
16 recovery of PSP amplitude could be produced by inserting a brief low-temporal frequency component in
18 ous G modification can be readily eliminated by inserting a capping step before the sulfurization rea
19 utant strain of Escherichia coli was created by inserting a cassette encoding sucrose sensitivity and
21 resonance energy transfer (FRET)-based probe by inserting a CFP after residue Ser-2367 and a YFP afte
22 ri from the U.S. and Japan, were constructed by inserting a chloramphenicol-resistant cassette into t
23 ly encoded GTP sensors, which we constructed by inserting a circularly permuted yellow fluorescent pr
24 uency, we established a knock-in mouse model by inserting a core Sgamma1 region into the first intron
25 f the charge-mediated magnetoelectric effect by inserting a Cu layer at the PMN-PT interface, the ele
26 containing 8-oxoG efficiently and accurately by inserting a cytosine across from the lesion and by pr
27 cid glycoprotein (SAGP) has been constructed by inserting a deletion in the sagp gene using the metho
28 unit can accommodate whole-domain insertions by inserting a domain into one of the designed loops.
31 n system (q2DES) can be artificially created by inserting a few unit cells of delta doping EuTiO3 at
32 ebral artery (MCA) which was produced either by inserting a filament via the external carotid artery
34 ith a reversible p53 knockout (p53(rev/rev)) by inserting a floxed transcriptional stop into the firs
38 crease DNA secretion in a nonpiliated strain by inserting a gene cassette with a strong promoter to d
39 man immunodeficiency virus (HIV) specificity by inserting a gene, CD4zeta, containing the extracellul
41 generated promoter IV mutant mice (BDNF-KIV) by inserting a GFP-STOP cassette within the Bdnf exon IV
42 n in which only the lpxK gene is inactivated by inserting a kanamycin cassette into the chromosomal c
44 ient parental strain, E. coli O157:H7 W6-13, by inserting a kanamycin resistance gene cassette (kan)
45 onstructed an isogenic H. pylori uvrB mutant by inserting a kanamycin-resistance cassette into uvrB a
47 ial chromosomes (PACs) are readily generated by inserting a loxP site-containing Tn10 minitransposon
48 rived a conditional inactivation mouse model by inserting a loxP-flanked ataxin-7 cDNA with 92 repeat
49 ) slowly converts to an inactive latent form by inserting a major part of its reactive center loop (R
50 ting the spatial location of the carboxylate by inserting a methylene adjacent to the aromatic ring,
52 e of the large-plaque phenotype was achieved by inserting a new copy of the A33R gene into the thymid
55 tivate a gene that encodes such an activator by inserting a plasmid-borne internal fragment of the pu
56 in vivo, we generated a novel murine allele by inserting a point mutation identified in a patient wi
57 n in T cell activation has been investigated by inserting a potential glycosylation site at V alpha r
58 and arbitrary steering of their propagation by inserting a predefined thin interlayer, composed of e
60 1 binds sequence specifically to 10-bp sites by inserting a recognition helix into the major groove o
61 er, efficient trans splicing can be restored by inserting a second AG dinucleotide downstream, which
62 ped an infectious model of molecular mimicry by inserting a sequence encompassing the immunodominant
63 local electron correlations control the 2DEG by inserting a single atomic layer of a rare-earth oxide
65 l of human PCN, we generated Myc transgenics by inserting a single-copy histidine-tagged mouse Myc ge
66 y this, we have perturbed the 3' UTR of Otx2 by inserting a small fragment of DNA from the lambda pha
68 '-cap dependence of translation was assessed by inserting a stable hairpin (HP) near the 5' end of th
69 ase (BioB) converts dethiobiotin into biotin by inserting a sulfur atom between C6 and C9 of dethiobi
70 n inducible promoter (flacp) was constructed by inserting a synthetic lac operator sequence between t
72 ork interrogates the identities of the peaks by inserting a time-of-flight mass spectrometry system i
73 rematurely truncated the Kcnq1ot1 transcript by inserting a transcriptional stop signal downstream of
74 p(-) HSV/AAV hybrid amplicon vector was made by inserting a transgene cassette flanked with AAV ITRs
75 e, an E7-deficient HPV-16 genome was created by inserting a translational termination linker (TTL) in
77 entation, which requires active exploration, by inserting a transparent plastic partition in the cent
78 as specifically abolished in two ways: first by inserting a ubiquitin monomer between NS2 and NS3, an
80 ng the residues surrounding this short motif by inserting additional hydrophobic residues creates ver
81 t al. created a human Dicer mutant cell line by inserting an AAV targeting cassette into the helicase
82 nce between the CRE and TATA box was changed by inserting an additional 5 or 10 bases, it was observe
83 polymerases replicate DNA containing 8-oxoG by inserting an adenine opposite the lesion; consequentl
84 ocessing of the latter protein was prevented by inserting an Ala at the native protease cleavage site
86 olysis by G protein alpha subunits, in part, by inserting an amino acid side chain into the site occu
87 report that water-solubility can be achieved by inserting an amino functionality in the N(9) side cha
88 3 bispecific antibody (BsAb) was constructed by inserting an anti-Her2 single-chain variable fragment
90 es ATP hydrolysis in the next catalytic site by inserting an Arg -finger into the site, as captured i
92 2-EHV-1-gD recombinant virus was constructed by inserting an EHV-1 gD expression cassette under the c
93 However, complete separation of E2 and p7 by inserting an encephalomyocarditis virus (EMCV) intern
94 diated fusion and its inhibition by antibody by inserting an epitope from human immunodeficiency viru
97 The cloned streptococcal dltA was disrupted by inserting an ermAM cassette, and then it was lineariz
98 bacterial artificial chromosome (BAC) clone by inserting an excisable BAC plasmid sequence in the lo
99 mouse Sca1 gene, we generated knock-in mice by inserting an expanded tract of 78 CAG repeats into th
101 epitope tagging of dimerization partners and by inserting an extra, unpaired Cys residue to create an
105 on of a conditional hemO mutant, constructed by inserting an isopropyl-beta-D-thiogalactopyranoside (
106 we generated a FLT3/ITD knock-in mouse model by inserting an ITD mutation into the juxtamembrane doma
107 n as a cis-acting mRNA destabilizing element by inserting an L-A viral -1 PRF signal into a PGK1 repo
108 ted lesions, we established a knock-in model by inserting an Sgamma2b region, a bona fide AID target,
110 nse RNAs." We have explored this possibility by inserting antisense RNAs targeted against one essenti
111 monkeys, while DA likely evolved from a Xiao by inserting approximately 200 kb of chimeric sequence f
112 nds is that of intercalators, which bind DNA by inserting aromatic moieties between adjacent DNA base
113 ity of nine different gap filling techniques by inserting artificial gaps into 20 automated Rsoil rec
114 unique opportunity to develop core isosteres by inserting B-N units in place of C horizontal lineC bo
115 y living bacterial cell array was fabricated by inserting bacteria into a microwell array formed on o
116 ransactivation by Sp1 in vivo was diminished by inserting bases between the AT-rich and GC-rich eleme
119 terrupts this triad in cofactor-bound AKR11A by inserting between the lysine and tyrosine side-chains
121 interfaces are generated from existing ones by inserting C-loops between the interaction motifs that
122 ate nonmuscle myosin heavy chain (NMHC) II-B by inserting cDNA encoding green fluorescent protein (GF
123 Attempts to control the reduction potential by inserting charged amino acids into the hydrophobic co
124 imeric SoNar protein was initially developed by inserting circularly permuted yellow fluorescent prot
125 simple assay for monitoring activity in vivo by inserting circularly-permutated GFP into conformation
126 el in the absence of membrane tension; thus, by inserting compounds that acquire a charge on exposure
127 We also analysed the receptor dimerisation by inserting cysteine residues at strategic positions ab
128 om biocatalysts built in a lego-like fashion by inserting, deleting, or exchanging native or foreign
129 rase that rescues damage-stalled replication by inserting deoxy-ribonucleotides opposite DNA damage s
130 at the hetero-interface can be readily tuned by inserting dielectric layers into the vdW gap, consist
133 two strands during primer extension, mainly by inserting dNMPs opposite unmodified templates or DNA
134 lipids to a uniform length before loading or by inserting each lipid so that it penetrates the groove
135 fficient genome maturation for a herpesvirus by inserting ectopic cleavage sites into the murine cyto
136 hese extremely high levels of VSG expression by inserting ectopic VSG117 into VSG221 expressing T. br
138 y, we take advantage of this immune response by inserting epitopes against which an antibody response
139 ndgap donor polymer, EI-PFDTBT, is developed by inserting ethylene bridging units to ensure a coplana
141 ompetent chimeric viruses can be constructed by inserting foreign antigenic sequences within the poli
142 he interacting surface(s) for these proteins by inserting fragments corresponding to the cytoplasmic
143 Graphite intercalation compounds are formed by inserting guest molecules or ions between sp(2)-bonde
145 we determined the topology structure of BCRP by inserting hemagglutinin (HA) tags in its predicted hy
147 om our cosmids, but infectivity was restored by inserting intact ORF63 into each mutated cosmid.
148 c loop 262-274 stabilizes the actin filament by inserting into a pocket formed at the interface betwe
149 are metastable proteins that mediate fusion by inserting into a target membrane and refolding from a
151 er suggests that (3)PN decays preferentially by inserting into an inward-pointing acetal C-H bond of
152 tein A-I with dimyristoylphosphatidylcholine by inserting into defects within the lipid surface, ther
153 y generate potentially deleterious mutations by inserting into genes, yet little is known about the c
156 boxy-terminal tails provide binding affinity by inserting into pockets on the 20S proteasome, and PA2
157 y alpha helix of complexin clamps exocytosis by inserting into SNARE-complexes support this hypothesi
158 transposons can escape epigenetic silencing by inserting into specific genomic locations, such as th
160 alpha-helix of Complexin-I inhibits release by inserting into the C-terminus of the SNARE complex.
162 Subsequently, the OSVP virus was constructed by inserting into the OSV viral genome a murine 15-prost
163 roup escapes contact with the aqueous milieu by inserting into the prenyl pocket and stabilizing the
164 g switch," which disrupts all four Rep genes by inserting into their shared coding region an intron t
165 cinoma cells initiate the metastatic cascade by inserting invasive pseudopodia through breaches in th
166 regulatory role of the URE was demonstrated by inserting it into the 3'-UTR of a beta-globin reporte
167 upstream region of sequence was carried out by inserting it into the pGL3 reporter gene vector for t
168 e PRDII site of the interferon-beta enhancer by inserting its DNA-binding segments (AT hooks) into th
169 ocks the intrinsic rotation of the nanomotor by inserting its N-terminal inhibitory domain at the sam
170 he S-adenosyl-l-methionine (AdoMet) cofactor by inserting its side chain into a narrow channel that r
171 rotransposition is known to create mosaicism by inserting L1 sequences into new locations of somatic
173 circulation have only simulated such events by inserting large amounts of fresh water into the north
174 ing the nucleotides 5" of the GAA repeats or by inserting linkers of 10 or 12 bp at a position 3" of
176 e produced a conditional lMTP knockout mouse by inserting loxP sequences flanking exons 5 and 6 by ge
183 levated levels of IFN-gamma mRNA and protein by inserting multiple copies of murine IFN-gamma genomic
184 peptide gene delivery agents were developed by inserting multiple cysteine residues into short (dp 2
185 have investigated DNA segregation in E. coli by inserting multiple lac operator sequences into the ch
187 needle penetration into skin was controlled by inserting needles with a rotary drilling device, whic
188 aPKCzeta may mediate choleretic effects by inserting NTCP into the plasma membrane, and nPKCepsi
189 ta (hPoliota) promotes translesion synthesis by inserting nucleotides opposite highly distorting or n
190 er (m-OPE) is modified to yield a "daughter" by inserting one nitrogen atom into the m-OPE core.
191 ee separate reporter constructs were created by inserting one or more fluorophores into different pos
192 wcastle disease virus (NDV) edits its P gene by inserting one or two G residues at the conserved edit
193 domain-swapped interfaces can be engineered by inserting one protein into a surface loop of another
194 between the EBNA1 sites of a functional pair by inserting or deleting 1 or 2 bp abolished replication
195 host specificity has recently been achieved by inserting or deleting genes that affect virus replica
196 ORF35 Flag-tagged recombinants were made by inserting ORF35-Flag at the nonnative Avr site as the
197 We illustrate the usefulness of our results by inserting our BKCa description into previously publis
198 ecognition, we modified the (-) DNA template by inserting overlapping locked nucleic acid (LNA) doubl
199 interaction, a Ct mutant P2Ct is constructed by inserting P2 in place of its C-terminal homologous si
200 ystem amenable to propagation in human cells by inserting pac, oriP, and EBNA1 elements into the vect
201 eterozygous for this oncogene were generated by inserting part of the human MYH11 cDNA into the mouse
202 esponded to a template TT (6-4) photoproduct by inserting predominantly an A opposite the 3' T of the
204 dent chromosomal disruptions were engineered by inserting promoterless, terminatorless kanamycin or c
205 promoters, a plasmid library was constructed by inserting random pieces of S. pneumoniae chromosomal
206 ered to be up to 0.35 in the visible regime by inserting relatively low amounts of As-content into t
207 nd a rep(+) HSV/AAV hybrid amplicon was made by inserting rep68/78 outside the rep(-) vector 3' AAV I
209 ic, cholestatic, and anticholestatic effects by inserting, retrieving, and inhibiting retrieval of tr
210 the presence of the STE, can be circumvented by inserting sequences containing a new translation init
211 (HIV-1), chimeric proteins were constructed by inserting sequences derived from the V3 loop of gp120
212 orter (COX2-3'-UTR-luciferase) was generated by inserting sequences within the 3'-untranslated region
213 eated an acid-resistant strain, Ty21a-AR-Ss, by inserting Shigella glutaminase-glutamate decarboxylas
214 Eight different chimeras were constructed by inserting simian immunodeficiency virus (SIV) sequenc
215 the Delta(632-644) deletion mutant phenotype by inserting similar Cys-rich sequences from Drosophila
216 ce, we have altered the translocated segment by inserting, singly, two different foreign epitopes.
217 These elements persist in the host genomes by inserting site-specifically into multicopy target sit
219 vitro, stem cells can readily be engineered by inserting specifically tailored transgenes with antit
220 gger) value can be raised quite dramatically by inserting "speed bumps" in the form of steric and/or
222 ts on the stability and folding of a protein by inserting stretches of 7 to 13 residues into a suitab
223 ion of >95% of the genes in Escherichia coli by inserting synthetic DNA cassettes and molecular barco
224 esign makes use of the biological structures by inserting synthetic materials and processes that augm
226 ntranslated region (3'-UTR) hybrid construct by inserting the 3'-UTR of p21(waf1/cip1) mRNA just down
227 p-independent component was further analyzed by inserting the 5' leader sequences into the intercistr
228 he host cells, we engineered a new immunogen by inserting the B subunit of Stx2 at the amino termini
229 The AcMNPV gp64 efp gene was inactivated by inserting the bacterial lacZ gene in frame after codo
230 of the P AUG nor blocking ribosomal scanning by inserting the BamHI-SL between the C and P start codo
232 uggest a model in which Tat binds to TAR RNA by inserting the basic recognition sequence into the maj
233 design involves thickness adjustable clothes by inserting the bent polymer sheets between two fabrics
234 n to the capillary outlet is achieved simply by inserting the capillary outlet containing the porous
235 The electrical connection is achieved simply by inserting the capillary outlet containing the porous
236 ed the identity of the galactosyltransferase by inserting the cDNA in frame into the genome of the me
237 zed the processing of an operon of this type by inserting the central part of mes-6/cks-1 into an SL2
239 ered in a series of steps, as we demonstrate by inserting the chromosome of phage lambda (minus a reg
240 for epitope-tagged CaM has been constructed by inserting the coding sequence for a nine amino acid p
241 which expresses the HCV structural proteins, by inserting the contiguous Core, E1, and E2 coding regi
242 ucture of ParG suggests that it binds to DNA by inserting the double-stranded beta-sheet into the maj
243 we describe two new alleles of Pax2 created by inserting the enhanced green fluorescent protein codi
244 C57BL/6 (B6) mice (RIP.B7-H4) were developed by inserting the entire B7-H4 open reading frame under t
246 oviruses was exploited using the PIV3 vector by inserting the foreign viral genes at the 3' terminus,
247 The FP sensor module was then assembled by inserting the FP sensor into a hole drilled in the wa
249 and trafficking, knock-in mice were created by inserting the gene encoding enhanced green fluorescen
250 f nsp2 to express foreign genes was examined by inserting the gene encoding green fluorescent protein
251 to enhance this content by plant breeding or by inserting the gene for ferritin into plants, eg, soyb
252 ion of 2.0-kb LAT open reading frames (ORFs) by inserting the gene for green fluorescent protein (GFP
253 nt of the virus lacking RBD is reestablished by inserting the hormone erythropoietin, infection remai
254 ion of hair cells in the adult mouse utricle by inserting the human diphtheria toxin receptor (DTR) g
255 previously established a yeast model system by inserting the human Ha-ras/HRAS1 minisatellite into t
256 erated luciferase-expressing transgenic mice by inserting the human ubiquitin C promoter coupled to t
257 mutant, S. cinnamonensis L1, was constructed by inserting the hyg resistance gene into a unique BglII
258 tion of neurofibromin, the NF1 gene product, by inserting the in-frame exon 23a into the region of NF
260 o gene encoding the large subunit of Rubisco by inserting the large and small subunit genes of the Se
261 Capsid completion does not necessarily occur by inserting the last building block into an incomplete,
262 ors carrying the whole FXN genomic DNA locus by inserting the luciferase gene in exon 5a of the FXN g
263 the usage of essential Fe-S cluster proteins by inserting the mevalonate pathway) can offset the indi
264 study has combined both of these strategies by inserting the murine IL-12 gene into a replication-co
265 as used to modify the influenza virus genome by inserting the p46-63 sequence of hen egg lysozyme (HE
266 expressed in transgenic tobacco chloroplasts by inserting the pagA gene into the chloroplast genome.
267 In one, a "knock-in" mutant was created by inserting the Puro-EGFP cassette into a noncoding reg
269 transposon inverted repeat (IR) elements and by inserting the RSV promoter (to drive expression of th
270 size effect can be only partially corrected by inserting the same size of stuffer DNA into both expe
271 rus (AAV) hybrid amplicon vector constructed by inserting the sequences of regulatory protein (rep) a
276 iptional activity of the SL3 LTR were tested by inserting the viral enhancer sequences into a plasmid
277 We activate UNC-86 transcription activity by inserting the VP16 activation domain into an unc-86 g
282 ry normally recruited to the site of budding by inserting this WW domain in different locations withi
283 ostic and therapeutic agent, was constructed by inserting three expression cassettes (encoding Renill
290 hrough a cis-syn thymine-thymine (T-T) dimer by inserting two As opposite the two Ts of the dimer.
291 h a cis-syn thymine-thymine dimer (TT dimer) by inserting two As opposite the two Ts of the dimer.
292 hese cyclic lanthanide complexes label cells by inserting two hydrophobic alkyl chains into cell memb
293 thodologically, these findings were obtained by inserting two microelectrodes at close proximity in t
294 :2 ratio) in Et2O at low temperature gives 3 by inserting two sulfur atoms into the Li-C (i.e., C2 an
300 tiated in the molecular dynamics simulations by inserting water molecules into the protein interior a
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