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1 subsequently incorporated into a full-length cDNA clone.
2 ome lineage expressed from a D. melanogaster cDNA clone.
3 d with in vitro transcripts of an infectious cDNA clone.
4 alanines by mutagenesis of an infectious FCV cDNA clone.
5 t background of a recombinant VSV infectious cDNA clone.
6 t RNA synthesis pattern was recovered from a cDNA clone.
7 to the LC coding region of an infectious FCV cDNA clone.
8 with a (32)P-labeled probe derived from the cDNA clone.
9 un cloned into a modified 1a/JFH1 infectious cDNA clone.
10 l VEEV strain originating from an infectious cDNA clone.
11 al of 68,721 ESTs were generated from 68,131 cDNA clones.
12 5' sequences from about half that number of cDNA clones.
13 enetics system to recover UUKV entirely from cDNA clones.
14 oncoding transcript represented by two IMAGE cDNA clones.
15 and derived 5' ESTs from >30,000 unique rat cDNA clones.
16 as proposed from the analysis of overlapping cDNA clones.
17 rtially sequenced over 5100 random T. reesei cDNA clones.
18 ymerase in all eight independently generated cDNA clones.
19 overed from the sequence of rhabdomyosarcoma cDNA clones.
20 t sequencing or pyrosequencing of individual cDNA clones.
21 re obtained from random sequencing of 16,152 cDNA clones.
22 collections contain principally two types of cDNA clones.
23 ae by multiple chromatographic steps, led to cDNA cloning.
24 transcription-polymerase chain reaction and cDNA cloning.
25 ikely an artifactual sequence created during cDNA cloning.
32 ese strategies--sequencing randomly selected cDNA clones, aligning protein sequences identified in ot
34 mutagenized p7 of an infectious genotype 1a cDNA clone and tested RNA transcripts of each mutant for
35 MaxT permutation analysis using t tests (20 cDNA clones and 10 unique genes), significance analysis
36 ), significance analysis for microarrays (33 cDNA clones and 15 genes, at an estimated false discover
38 port the generation of infectious SFTSV from cDNA clones and demonstrate that the behavior of recombi
39 this system is effective for both individual cDNA clones and for cDNA libraries, permitting the direc
40 erformed a gain-of-function screen of 15,779 cDNA clones and identified 40 genes affecting exon 18 of
41 se that will aid creation of functional NPHV cDNA clones and other novel tools for hepacivirus studie
42 sion in lymphocytes determined by sequencing cDNA clones and quantifying 6FAM-labeled PCR products fo
45 sing differential RNA selection, full-length cDNA cloning and 454 transcriptome sequencing of the ric
48 un-based approach that led ultimately to the cDNA cloning and functional characterization of many of
50 ed the expression of four of the isoforms by cDNA cloning and mass spectrometric analysis of proteins
57 chniques; functionally related sets of human cDNA clones; and genome-scale gene collections for Sacch
62 ibe the construction of the first human PARG cDNA clone by reverse transcription of CF3 human fibrobl
65 SOURCE provides content both in gene and cDNA clone-centric pages, and thus simplifies analysis o
67 ription-PCR and assembled into a full-length cDNA clone, clone C, which contained 14 mutations compar
70 Subsequent studies showed that the human cDNA clone complemented an ATR-deficient bacterial mutan
72 and JHM.WU and, utilizing these full-length cDNA clones, constructed chimeric viruses and mapped the
73 titutional effort to identify and sequence a cDNA clone containing a complete ORF for each human and
75 solated and characterized various myotrophin cDNA clones corresponding to the multiple transcripts by
76 Ps results in matches to previously reported cDNA clones, CP2, CP3, and CP4, as well as another seque
78 titution of G1100D in an infectious DA virus cDNA clone demonstrated a major role for this mutation i
81 mutation introduced into an infectious DENV2 cDNA clone did not yield detectable virus by plaque assa
83 created by transfection of a genotype I HDV cDNA clone directly into the liver of a woodchuck that w
84 ill summarize the major collections of human cDNA clones, discuss some limitations common to most of
86 with the EP4 receptor as a bait identified a cDNA clone encoding a 669-amino acid protein, designated
87 cles could be rescued from a full-length FCV cDNA clone encoding a nonfunctional VP2 when VP2 was pro
90 uences identified a single full-length maize cDNA clone encoding all the peptide sequences obtained f
92 ing the methods of proteomics, a full-length cDNA clone encoding an enzyme matching the properties of
94 s of a zebrafish cDNA library, we isolated a cDNA clone encoding receptor activity-modifying protein
96 omplementary DNA (cDNA) library to isolate a cDNA clone encoding the ferritin heavy chain polypeptide
100 genomes were constructed from two infectious cDNA clones encoding a virulent and an attenuated isolat
101 interaction of CB3-RD with DAF, we produced cDNA clones encoding both CB3-RD and CB3-Nancy and mutat
103 We isolated several related but distinct cDNA clones encoding novel structure proteins (NSP) when
104 right Yellow-2 protoplasts identified single cDNA clones encoding proteins that interact with either,
108 the complementary DNA (cDNA) for eNOS and 23 cDNA clones encoding the Asp-His-His-Cys motif (DHHC) pa
112 of 19-kD alpha-zein genes, we characterized cDNA clones encoding these proteins from a developing en
113 t here the isolation of a complementary DNA (cDNA) clone encoding one such enzyme, mannan synthase (M
116 nify analogous doubly spliced Rej mRNAs, and cDNA clones expressing two of them also showed Rej activ
118 We have identified this factor as the novel cDNA clone FL14676485 that encodes for the human hypothe
121 be used at any scale, from the isolation of cDNA clones for a particular gene of interest, to the im
124 teins led to the identification of a 4.75-kb cDNA clone from a Strongylocentrotus purpuratus ovary cD
128 ea mays, including ESTs representing 484,032 cDNA clones from 53 libraries and 36,565 fully sequenced
130 , rhinophores), we isolated five full-length cDNA clones from an A. californica central nervous syste
132 ecules, MHC class I genes were identified in cDNA clones from Arabian horse A2152, which presented bo
133 striction enzymes, nor the sequencing of 130 cDNA clones from benign and malignant breast tissue and
135 ions against a microarray consisting of 2173 cDNA clones from five pine expressed sequence tag librar
136 echniques, we measured mRNA levels in 11 283 cDNA clones from the cerebral cortex of Tg2576 mice and
139 Differential display was used to isolate cDNA clones from the three species showing differential
143 I), and salmon GnRH (sGnRH) were isolated by cDNA cloning from the brain of the Atlantic croaker, Mic
145 gene encoding Cl.ir channels in heart, ClC-2 cDNAs cloned from rat (rClC-2) and guinea pig (gpClC-2)
146 ions were found in CD247 complementary DNAs (cDNAs) cloned from the patient as well as in cDNA and ge
147 mutations at M-58 and E-191 in the chimeric cDNA clones further improved the viability of the chimer
151 the coding region of genes, and full-length cDNA clones have proven to be useful for investigation o
153 etermine the function of CBP, an orthologous cDNA clone (Hs CBP) was isolated from the sugar beet cys
157 m a single human fetal fast skeletal TnTbeta cDNA clone in order to circumvent the problem of N-termi
158 tions were introduced into an infectious FCV cDNA clone in order to evaluate the functional importanc
163 umber alterations across 42,000 mapped human cDNA clones, in a series of 54 gliomas of varying histog
180 bovine virulence, we assembled an infectious cDNA clone of an RGD-containing A(24)Cru and derived mut
182 th open reading frame (ORF) of an infectious cDNA clone of HCV (genotype 1a, H77 strain) in the nontr
185 truction and the properties of a full-length cDNA clone of HRV16, pR16.11, which produces in vitro tr
187 ced, polyadenylated BART RNAs, a full-length cDNA clone of one of the BART isoforms was obtained and
189 ogical disease, we constructed a full-length cDNA clone of silver-haired bat-associated RV (SHBRV) st
190 In this study, we constructed a full-length cDNA clone of SL-CoV WIV1 (rWIV1), an ORFX deletion muta
191 constructs engineered based on an infectious cDNA clone of T36 isolate of CTV, including hybrids cont
194 nt wild-type 88-1961 strain (r88) and from a cDNA clone of the highly attenuated Jeryl Lynn vaccine s
195 aring the 197-aa deletion from an infectious cDNA clone of the highly virulent PEDV PC22A strain (inf
197 ting of combinations of genes derived from a cDNA clone of the neurovirulent wild-type 88-1961 strain
199 e these mutants, we engineered an infectious cDNA clone of VSV and employed N-RNA templates from thos
200 g sequences were inserted into a full-length cDNA clone of VSV, and the virus recovery, kinetics of g
201 We first constructed a stable full-length cDNA clone of ZIKV in a novel linear vector from which i
203 th IFN sensitivity and virulence, infectious cDNA clones of a virulent North American strain and the
206 transiently in HEK293 cells using expression cDNA clones of human alpha1C, alpha2delta, and beta subu
210 ectious virus was recovered from each of the cDNA clones of the HN glycoprotein mutants, employing a
213 t on the purification, characterization, and cDNA cloning of a novel UGAT involved in the biosynthesi
215 from 53 libraries and 36,565 fully sequenced cDNA clones, out of which 31,552 clones are non-redundan
217 es rapid subtraction hybridization (RaSH) of cDNA clones prepared from two cell populations, a driver
220 agenesis was performed to generate three new cDNA clones (pSHEV-1, pSHEV-2, and pSHEV-3) which differ
221 tr1 by transfection with a recombinant hCtr1 cDNA clone reduced endogenous hCtr1 mRNA levels, whereas
223 ional analyses of the CHV-1/EP713 infectious cDNA clone, reported here, define the requirements for p
232 ies and highlights discrepancies between any cDNA clone sequence and its expected reference sequence.
238 identify the RNA activators, we developed a cDNA cloning strategy based on stringent affinity of RNA
240 MSPE was used to isolate a novel full-length cDNA clone that encodes a 66-kDa murine G+C-rich promote
241 was screened resulting in the isolation of a cDNA clone that encodes a 738 amino acid protein (SmSmad
242 in-conjugating enzyme, have isolated a human cDNA clone that may function as a signal peptidase and h
243 Based on this screening, we identified a cDNA clone that was allowing yeast cells to grow in the
245 A libraries for the isolation of full-length cDNA clones that are not yet available in the public dom
246 s transformed with a human cDNA library, and cDNA clones that rescued growth at low K(+) concentratio
247 KCC1 gene, we mapped the 5' end of the KCC1 cDNA, cloned the corresponding genomic DNA, and identifi
248 he mutagenesis of the CHV-1/EP713 infectious cDNA clone to define the requirements for p29 and p48 cl
249 unya fever (CHIKF) pandemic, we used an EILV cDNA clone to design a chimeric virus containing the chi
250 toprotein (AFP) genes by hydridizing labeled cDNA clones to HpaII and MspI digests of DNA from differ
253 R products representing assemblies of BAC or cDNA clones typically require maintenance, propagation,
254 om cultured cells and identified full-length cDNA clones using amino acid sequences from internal pep
256 Screening with a microarray containing 864 cDNA clones using wild-type and brn-3b (-/-) retinal cDN
269 of these cleavage sites in an infectious FCV cDNA clone was lethal for the virus, indicating that the
271 cDNA microarray containing 7,712 macrophage cDNA clones was used to compare the transcriptional prof
272 mparative genomic DNA sequence analysis, and cDNA cloning, we found that the mouse clade B cluster at
275 ssion subtraction cDNA library from which 11 cDNA clones were found by differential dot blot hybridiz
279 nal genes in the 881-kb contig; 11 groups of cDNA clones were identified in addition to those for S2-
280 e bacterial IF3, several partially sequenced cDNA clones were identified, and the complete sequence w
293 ption-polymerase chain reaction (RT-PCR) and cDNA cloning were conducted by conventional procedures.
295 We previously isolated a partial soybean cDNA clone whose transcript abundance is increased upon
296 oarrays were generated that carry about 8500 cDNA clones with approximately 6000 obtained from mammar
297 s, we constructed a series of BUNV S segment cDNA clones with deletions in the 3' and/or 5' UTR and t
298 rsion of our strategy that can be applied to cDNA clones with large cloning vectors, thereby overcomi
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