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1 cDNA array and tumor microarray analysis shows that mRNA
2 cDNA with 5 A's may yield novel Gag product(s), while cD
3 cDNAs for Aggregate Spider Glue 1 (ASG1) and 2 (ASG2) ha
4 cDNAs were constructed by site-directed mutagenesis that
5 ased microfluidics was used to generate 1000 cDNA libraries, each from an individual pancreatic islet
6 ect as low as 200 ymol, corresponding to 120 cDNA molecules of L-gene Ebola virus with a limit of det
7 re we describe a method that allows up to 25 cDNAs to be assembled into a single baculoviral expressi
10 ls transfected with human Kv7.2 and/or Kv7.3 cDNAs revealed that each of these four mutations stabili
12 g enzymes prior to rapid amplification of 5' cDNA ends (5' RACE) for HIV-1 RNA and quantitative rever
15 crosomal, seed-specific class A LPAT2s and a cDNA from C. avigera var. pulcherrima (CpuLPATB) encodin
19 ing to VPE was found to be up-regulated in a cDNA-AFLP analysis of host responses of a wild peanut, A
23 viral gene expression vector transferring a cDNA encoding an enzyme or channel protein, and targetin
26 cells that were modified with the human ADA cDNA (MND-ADA) gamma-retroviral vector after conditionin
27 f iCLIP and related methods that can amplify cDNAs that truncate at crosslink sites and we show that
30 geted capture protocol to enrich VZV DNA and cDNA prior to whole-genome/transcriptome sequence analys
31 ng suppression subtractive hybridisation and cDNA libraries of cotton genotypes tolerant to Verticill
32 ment; hence, subsequent adapter ligation and cDNA amplification steps are exclusively applied to cP-c
33 encing with methylation-specific primers and cDNA analysis in patient neurons indicated selective exp
34 c 346 kb inversion in multiple probands, and cDNA sequencing and a splicing assay established that tw
38 sing a CAV1 scaffolding domain construct and cDNAs encoding wild-type CAV1, and CAV1 phosphorylation
42 ificant conformational change of the aptamer-cDNA than the pure aptamer upon binding with P4, as conf
43 nts based on expression technologies such as cDNA microarrays, RNA-Seq, and cell imaging-based assays
44 with various expression technologies such as cDNA microarrays, RNA-Seq, and cell imaging-based assays
45 ed expanded screening of eutherian and avian cDNA libraries using yeast-two-hybrid and split-ubiquiti
46 ale (Eubalaena glacialis; family Balaenidae) cDNA derived from long-wavelength sensitive (LWS) cone o
48 e DNA synthesis and release 3'-azido-blocked cDNA fragments in a process akin to dideoxy-Sanger seque
50 the AG haplotype also results in greater blw cDNA expression and a significant decrease in lifespan r
51 Furthermore, RT-qPCR analysis of stapes bone cDNA showed that SERPINF1-012 expression is reduced in o
55 ysis of finger millet (Eleusine coracana) by cDNA subtraction identified drought responsive genes tha
57 Overexpression of p21(WAF1/CIP1), either by cDNA transfection or clinical drugs, preferentially impa
58 Gain and loss of function experiments by cDNA and siRNA transfection revealed profound effects of
60 ybrid screenings of placenta and lung cancer cDNA libraries, which demonstrated that the long IDR lin
62 en using dysbindin as bait against a cardiac cDNA library to identify the cardiac dysbindin interacto
63 TALEN technology and a 7bp deletion in CERKL cDNA that caused the premature termination of CERKL.
64 the endogenous fusion protein or a chimeric cDNA leads to the formation of indolent liver tumors in
66 sgene, TgAC1, consisting of Clrn1-UTR (Clrn1 cDNA including its 5' and 3' UTR) under the control of r
68 now find that this is caused by constrained cDNA-ends, which can result from the sequence and struct
72 id DCK gene modification, gRNA resistant DCK cDNA was created by the introduction of silent mutations
73 mutation introduced into an infectious DENV2 cDNA clone did not yield detectable virus by plaque assa
74 support the concept of retroelement-derived cDNA as key triggers of systemic autoimmunity in Trex1-d
75 ion sequencing on single glomus cell-derived cDNAs to eliminate contamination of genes derived from o
76 icon photonic microring resonators to detect cDNA reverse transcription products via a subsequent enz
78 hput method, we developed a T7 phage display cDNA library derived from mRNA isolated from bronchoalve
79 ed lentivirus-based human complementary DNA (cDNA) library, transfected the cells with HCV subgenomic
82 y noted, the positions of complementary DNA (cDNA)-starts depend on cDNA length in several iCLIP expe
84 ions were found in CD247 complementary DNAs (cDNAs) cloned from the patient as well as in cDNA and ge
89 insertion of IRAlu at the 3'-UTR of the EGFP cDNA led to a rhythmic circadian nuclear retention of th
90 PSE, which are orthologs of M-IPSE, from egg cDNA of S. haematobium Using PCR and immunodetection, we
91 unya fever (CHIKF) pandemic, we used an EILV cDNA clone to design a chimeric virus containing the chi
92 co-expression of 14-3-3- and AtWRI1-encoding cDNAs led to increased oil biosynthesis in Nicotiana ben
95 iopsies were taken and total RNA extraction, cDNA synthesis and PCR was performed using 10 candidate
96 isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed,
98 tected in less than 2 h without the need for cDNA synthesis or any other enzymatic reactions and with
100 eps follow published methods (Smart-seq2 for cDNA synthesis and Nextera XT barcoded library preparati
103 port the generation of infectious SFTSV from cDNA clones and demonstrate that the behavior of recombi
109 ection of cultured neurons with human GRIN2D cDNA harboring c.1999G>A leads to dendritic swelling and
113 cellular expression systems use heterologous cDNA-based vectors which express proteins well above phy
115 DBR1) is not required for early steps in HIV cDNA formation but is necessary for synthesis of interme
122 cDNAs) cloned from the patient as well as in cDNA and genomic DNA from other individuals, suggesting
123 iation and mismatches of alleles detected in cDNA and gDNA suggesting that some loci have undergone p
126 leotide polymorphism (SNP) discrimination in cDNAs from human breast cancer cell lines, which makes s
127 unreported LPATs were identified, including cDNAs from Cuphea viscosissima (CvLPAT2) and Cuphea avig
129 ice genotype (Hasawi), a salt-stress-induced cDNA expression library was constructed and subsequently
130 aring the 197-aa deletion from an infectious cDNA clone of the highly virulent PEDV PC22A strain (inf
133 underlying molecular mechanism for inhibited cDNA synthesis, we developed a deep sequencing strategy
134 SpISO-seq requires less than 1 ng of input cDNA, limiting or removing the need for prior amplificat
135 with the reverse transcription of mRNA into cDNA, a process that can introduce strong signal variati
136 ntrol groups) for reverse transcription into cDNA, preamplification and then real time quantitative p
137 -containing rhodopsin (sgRho) vs. intronless cDNA in ameliorating retinal disease phenotypes in a rho
142 ced, polyadenylated BART RNAs, a full-length cDNA clone of one of the BART isoforms was obtained and
143 In this study, we constructed a full-length cDNA clone of SL-CoV WIV1 (rWIV1), an ORFX deletion muta
144 We first constructed a stable full-length cDNA clone of ZIKV in a novel linear vector from which i
149 To address this we have cloned a full-length cDNA encoding the P. xylostella RyR and established clon
155 fication of cDNA ends (RACE) and full-length cDNA sequencing, revealed four independent promoters, pr
157 e isolated and characterized the full-length cDNA, gDNA and a putative promoter of a RIOK-2 protein k
161 and Pacific Biosciences (PacBio) full-length cDNAs to identify 104,091 high-confidence protein-coding
162 y enriched and sequenced copepod full-length cDNAs, which led to the characterization of copepod tran
163 T primers and processed into adapter-ligated cDNA libraries that were sequenced using an Illumina pla
164 f lncRNAs in human CML cells using an lncRNA cDNA microarray and identified an lncRNA termed lncRNA-B
165 the mouse COX-2 gene upstream of luciferase cDNA to characterize COX-2 basal transcriptional regulat
166 ntiviral complementation with wild-type MDH2 cDNA restored MDH2 levels and mitochondrial MDH activity
167 ecome the gold standard technique to measure cDNA and gDNA levels but the resulting data can be highl
168 hybridization-based targeted RNA-Seq method, cDNA-smMIPs are a cost-effective high-throughput tool fo
170 enerated recombinant proteins from the mouse cDNAs encoding the Hsp90alpha-Delta and wild-type Hsp90a
172 ns, most of which were supported by multiple cDNA evidence (72%) while only 20% of them have coding c
173 nes RNA barcoding and generation of multiple cDNA copies per RNA molecule to eliminate errors introdu
177 s to control viral replication we cloned Mx1 cDNAs from three bat families, Pteropodidae, Phyllostomi
179 with AI and OCA6 into human SLC24A4 (NCKX4) cDNA leading to single residue substitutions in the muta
184 licing patterns using rapid amplification of cDNA ends (RACE) and full-length cDNA sequencing, reveal
185 stem combined with 5' rapid amplification of cDNA ends (RACE), in vitro transcription assays, real-ti
197 y annotated using morphological profiling of cDNA constructs, via a microscopy-based Cell Painting as
198 at enable highly multiplexed resequencing of cDNA target regions of approximately 100 nucleotides and
200 activation domain of YAP1, and sequencing of cDNA from the patient shows it does not result in nonsen
201 reparation and high-throughput sequencing of cDNA libraries from samples of small RNA is a powerful t
203 eloped kinetic cross-linking and analysis of cDNAs (chiCRAC), an ultraviolet cross-linking method tha
204 us-based system allows the overexpression of cDNAs of up to 2,100 nucleotides (encoding a protein of
205 contrast, we show that a broad size range of cDNAs in iCLIP allows the cDNA-starts to efficiently del
206 of complementary DNA (cDNA)-starts depend on cDNA length in several iCLIP experiments and we now find
210 his EB phenotype was fully rescued by BAC or cDNA complementation but not by the reduction of p53 lev
212 ulties in cloning the full-length RPGR-ORF15 cDNA that includes a purine-rich 3'-coding region; howev
216 erent graft combinations was used to prepare cDNA libraries for small RNA sequencing and to analyze m
217 single-molecule molecular inversion probes (cDNA-smMIPs) that enable highly multiplexed resequencing
218 d RNA is then reverse-transcribed to produce cDNA, with SHAPE-modified bases leading to truncated cDN
219 body-producing B cell clones at the protein, cDNA and gDNA levels, we characterized additional LAIR1
221 We deduced the full-length of the RBM28 cDNA sequence and profiled its expression patterns detec
222 icry or by overexpression a miR827-resistant cDNA of NLA produced the opposite phenotype of reduced p
224 etion with RNA interference (RNAi)-resistant cDNA constructs demonstrated that VLP formation required
226 d RNA and PCR amplification of the resultant cDNA with gene-specific primers demonstrated the presenc
230 In vivo assays show that this hybrid RNA-cDNA molecule is required for mutagenic transposition, r
232 We show that a single delivery of the sans cDNA by the adenoassociated virus 8 to the inner ear of
234 activity in cells transfected with SERPINB7 cDNA carrying the mutation and promoted full-length SERP
235 ed transcriptome profiling and gene-specific cDNA-pyrosequencing have documented that transcriptome s
238 e FTIR analysis of extracted RNA, ds-DNA, ss-cDNA and isolated nuclei, we verified that the spectral
241 q method that does not involve second-strand cDNA synthesis, as we Directly Ligate sequencing Adaptor
243 ion of its RNA genome into a double-stranded cDNA copy, which is then integrated into the host cell c
244 ssay multiplex PCR-amplified single-stranded cDNAs and easily analyse and display the captured data.
246 erent ratios of rat alpha4 and beta2 subunit cDNA were transfected into human embryonic kidney 293 ce
247 restingly, titrating gamma2 or delta subunit cDNA levels progressively altered GABA-evoked currents,
248 unit degradation, 10-fold less delta subunit cDNA was required to recapitulate gamma2 subunit express
249 ng technology combined with Primer ID-tagged cDNA primers to efficiently quantify HIV-1 splicing at a
250 We were able to detect amplified target cDNA in all 5 cases of enteric fever; no detectable sign
251 s of higher thermal stability with cRNA than cDNA, although destabilized compared to duplexes of stan
256 road size range of cDNAs in iCLIP allows the cDNA-starts to efficiently delineate the complete RNA-bi
257 were caused by the plasmid backbone and the cDNA of the transgene, but not the intron-containing tra
258 Here, we adapt this method to direct the cDNA synthesis specifically toward the 3UTR/poly(A) tail
260 ive potassium channel toxins (KTxs) from the cDNA library of M. eupeus venom glands, and we compare t
262 g the pDNA-nMgO complex and detection of the cDNA was accomplished by measuring changes in PL intensi
264 s gene expression and it was proved that the cDNA fragment was relevant to the cellulose biosynthesis
266 tomato phytaspase genes were identified, the cDNAs were cloned and the recombinant enzymes were obtai
270 ation, ordering of scaffolds by alignment to cDNA sequences, incorporation of other map and sequence
272 uplexes via a linker; reverse transcription; cDNA library amplification; and finally high-throughput
273 ll-length Arabidopsis thaliana transketolase cDNA under the control of the cauliflower mosaic virus 3
274 otype after re-expression of wild-type TRMT5 cDNA in cells derived from the affected individuals.
277 rrent gene delivery strategies routinely use cDNA-based vectors for gene targeting; however, inclusio
285 ugh the combined effects of inhibiting viral cDNA production and cytidine-to-uridine-driven hypermuta
286 troduction of marks of DNA damage into viral cDNA, and thereby prime it for processing by DNA repair
287 troviruses must integrate their linear viral cDNA into the host genome for a productive infection.
289 IV-1 integrase (IN) for integration of viral cDNA into human chromosome is established, studies with
290 A3H, exhibited extensive comutation of viral cDNA, as determined by the frequency of G-to-A mutations
292 a tetramer or octamer complex with the viral cDNA long terminal repeat (LTR) ends termed an intasome.
293 ce in situ hybridization with a set of wheat cDNAs allowed the macrostructure and cross-genome homoeo
294 5 A's may yield novel Gag product(s), while cDNA with an extra base, 7 A's, may only be a minor cont
295 profiles were compared by using genome-wide cDNA microarrays and label-free quantitative proteomics,
296 ntaining fluorinated bases form hybrids with cDNA/RNA with slightly lower stability compared to that
298 a sativa by seed-specific co-expression with cDNAs for various Cuphea FatB acyl-acyl carrier protein
299 nels, we employed HEK cells transfected with cDNAs encoding three requisite receptor subtypes: alpha7
300 ovel transfection competition assay with ZnT cDNAs to confirm that the driving force for heterodimer
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