ライフサイエンスコーパス: cadherin-11

1 al fibroblast MMP production is regulated by cadherin 11.

2 ion of beta-catenin as well as the oncogenic cadherin 11.

3 ated with downregulation of beta-catenin and cadherin 11.

4 e FLS express other cadherins in addition to cadherin 11.

5 roteins were determined to be N-cadherin and cadherin 11.

6 so expressed a 115-kDa mesenchymal cadherin, cadherin-11.

7 the transmembrane and cytoplasmic domains of cadherin-11.

8 ly in part through the precise regulation of Cadherin-11.

9 nct characteristic of fibroblasts expressing cadherin-11.

10 Transfected cadherin-11 also localizes to focal adhesions in differe

11 e anti-inflammatory agent celecoxib can bind cadherin-11, an adhesion molecule important in rheumatoi

12 members and demonstrated distinct roles for cadherin-11 and cadherin-13 in synapse development.

13 However, coexpression of wild-type cadherin-11 and the splice variant promotes a dramatic i

14 nes expressing wild-type cadherin 11, mutant cadherin 11, and empty vector-transfected controls.

15 migration marker genes including Periostin, Cadherin 11, and Mmp2 while repressing markers of valve

16 nstrate that FLS express both N-cadherin and cadherin 11, and suggest that these cadherins are not co

17 A set of nine genes that included GPCR11, cadherin 11, annexin A1, vimentin, lactate dehydrogenase

18 dherins using either anti-N-cadherin or anti-cadherin 11 antibodies suggests that these cadherins are

19 ing domains (CBS) in the cytoplasmic tail of cadherin-11 are required for cell migration and invasion

20 ipose tissue inflammation and thus highlight cadherin-11 as a potential therapeutic target for the ma

21 We identified cadherin-11 as essential for the development of the syno

22 Exogenous wild-type cadherin-11 association with and membrane recruitment of

23 asts can be targeted by mAb directed against cadherin-11 (cad-11), a mesenchymal cadherin that fibrob

24 Cadherin-11 (Cad-11, also known as OB cadherin or CDH11)

25 Expression of human cadherin-11 (Cad11), also known as osteoblast cadherin,

26 However, despite the lack of cell surface cadherin 11, cadherin 11-null mouse FLS cells still form

27 We developed L cell transfectants expressing cadherin-11, cadherin-11 fusion proteins, and anti-cadhe

28 three of the ten candidates in human urine: cadherin 11 (CDH11), macrophage mannose receptor C1 (MRC

29 a1 and its downstream effector, OB-cadherin [cadherin 11 (CDH11)], regulate porcine myofibroblast phe

30 Cadherin-11 (CDH11) is increased in wound healing and fi

31 In focal adhesions, cadherin-11 co-localizes with beta1-integrin and paxilli

32 hered to cadherin-11-Fc, and transfection of cadherin-11 conferred the formation of tissue-like sheet

33 ehavior of L cells stably transfected with a cadherin 11 construct that lacked the juxtamembrane cyto

34 und pool of beta-catenin associated with the cadherin-11 cytoplasmic domain.

35 Together, these data implicate the cadherin-11 cytoplasmic JMD as a regulator of alpha-cate

36 Cadherin-11-deficient mice displayed increased stromal p

37 Cadherin-11-deficient mice have a hypoplastic synovial l

38 Higher expression levels of IL-33 in cadherin-11-deficient mice mediated ILC2 activation, res

39 nt with reduced adipose tissue inflammation, cadherin-11-deficient mice were protected from obesity-i

40 Furthermore, the actin cytoskeleton in cadherin-11 deltaJMD cells revealed a more extensive cor

41 rcalation into monolayers reflecting reduced cadherin-11-dependent cell motility on other cells.

42 Thus, synovial cadherin-11 determines the behavior of synovial cells in

43 Additionally, cleavage of cadherin-11 does not affect binding to beta-catenin and

44 proteases cleave the extracellular domain of cadherin-11 during CNC migration.

45 Cadherin 11 engagement stimulates increased synthesis of

46 To mimic cadherin 11 engagement, human RA synovial fibroblasts we

47 This up-regulation required cell cadherin 11 engagement, since a mutant Cad-11-Fc with re

48 gan culture system, we provide evidence that cadherin-11 expressed in fibroblast-like synoviocytes pl

49 Cadherin-11-expressing cells exhibit modest changes in c

50 The downregulation of cadherin-11 in cadherin-11-expressing metastatic PC3 cells decreases ce

51 cluding MMP-7 and MMP-15, are upregulated in cadherin-11-expressing, but not in cad11-DeltaJMD-expres

52 Cadherin 11 expression in rheumatoid synovial tissue was

53 Cadherin 11 expression was observed at points of cell-ce

54 These results suggest that cadherin-11 expression may be well correlated with the i

55 Interestingly, cadherin-11 expression was also detected via immunohisto

56 with a chimeric construct consisting of the cadherin 11 extracellular domain linked to the human IgG

57 Furthermore, treatment with the cadherin 11-Fc fusion protein diminished the invasive ca

58 st-like synoviocytes treated with a blocking cadherin 11-Fc fusion protein or control immunoglobulin

59 blast-like synoviocyte organ cultures with a cadherin-11-Fc fusion protein efficiently abrogated lini

60 FLS adhered to cadherin-11-Fc, and transfection of cadherin-11 conferre

61 Here, we cloned cadherin-11 from human rheumatoid arthritis (RA)-derived

62 We provide evidence that Cadherin-11 functions as target of the Prdm8/Bhlhb5 repr

63 L cell transfectants expressing cadherin-11, cadherin-11 fusion proteins, and anti-cadherin-11 mAb.

64 reates a fusion gene in which the osteoblast cadherin 11 gene (CDH11) promoter region on 16q22 is jux

65 Cadherin 11 has recently been identified on fibroblast-l

66 Absence of cadherin 11 in a mouse rheumatoid arthritis (RA) model l

67 Abundant connexin 43 and cadherin 11 in pellets demonstrated cell-cell junctions

68 these in vitro studies implicate a role for cadherin 11 in promoting cell invasion and contribute in

69 To examine the role of cadherin 11 in regulating the invasive behavior of fibro

70 Expression of both N-cadherin and cadherin 11 in the synovial lining was demonstrated by i

71 The downregulation of cadherin-11 in cadherin-11-expressing metastatic PC3 cel

72 Here, we show that expression of cadherin-11 in cadherin-11-negative C4-2B4 cells increas

73 Together, these studies implicate cadherin-11 in synovial tissue and lining layer formatio

74 antigen (LFA)-1], alpha4beta1 integrin, and cadherin-11 in the development of synovial inflammation.

75 These findings support a key role for cadherin-11 in the specific adhesion of FLS and in synov

76 the expression of the mesenchymal cadherin, cadherin-11, in breast cancer cell lines.

77 Cadherin 11 is a homophilic cell-to-cell adhesion molecu

78 Immunohistochemical analysis revealed that cadherin 11 is abundantly expressed in cells at the cart

79 N-cadherin, P-cadherin, and the mesenchymal cadherin-11 is actually elevated in invasive breast canc

80 The "mesenchymal" cadherin-11 is expressed in the CNC during migration yet

81 At the time of mesenchymal induction, cadherin-11 is expressed in the mesenchyme but not in th

82 However, the mechanism by which cadherin-11 is involved in this process is not known.

83 Cadherin-11 is localized to a detergent-soluble pool and

84 Immunocytochemistry shows that cadherin-11 is localized to the cell membrane at sites o

85 Cadherin-11 is unique among cadherins in that it exists

86 In particular, cadherin-11 is upregulated during tumour and inflammator

87 We found that expression of a mutant cadherin-11 lacking the cytoplasmic juxtamembrane domain

88 Here, we show that cadherin-11 localizes to focal adhesions and promotes ad

89 Importantly, anti-cadherin-11 mAb blockade similarly improved inflammation

90 in-11, cadherin-11 fusion proteins, and anti-cadherin-11 mAb.

91 Cadherin-11 may mediate the interaction between malignan

92 We found that cadherin-11 mediated fibroblast-like synoviocyte cell-to

93 e embryo, suggesting that a defined level of cadherin-11-mediated cell adhesion is required for migra

94 Here, we show that mesenchymal cadherin-11 modulates stromal fibroblast function.

95 Cadherin-11 mRNA and protein, as well as a variant form,

96 generated L cell clones expressing wild-type cadherin 11, mutant cadherin 11, and empty vector-transf

97 e, we show that expression of cadherin-11 in cadherin-11-negative C4-2B4 cells increases their spread

98 These observations suggest that cadherin-11 not only provides a physical link between PC

99 determined in FLS derived from wild-type and cadherin 11-null mice using immunofluorescence (IF), bio

100 from wild-type mice and 1 in FLS sample from cadherin 11-null mice.

101 espite the lack of cell surface cadherin 11, cadherin 11-null mouse FLS cells still formed intimate c

102 obox 1, loss of E-cadherin, up-regulation of cadherin 11, p120 isoform switching, activation of extra

103 ates including itself, fibronectin, ephrinB, cadherin-11, pcdh8 and pcdh8l (this work).

104 herin-11 splice variant promotes invasion of cadherin-11-positive breast cancer cells.

105 Cleavage of cadherin-11 produces an extracellular fragment that prom

106 mediated by FGF receptor signaling; and that cadherin-11 promotes epithelial cell motility in a manne

107 We propose that ADAM cleavage of cadherin-11 promotes migration by modifying its ability

108 ansferase 1 (DNMT1) and a 6-fold decrease in cadherin 11 protein expression.

109 ent also resulted in increased expression of cadherin-11 protein in human tumor biopsies in three out

110 suggest that stromal fibroblasts expressing cadherin-11 regulate adipose tissue inflammation and thu

111 derscore the existence of a pathway by which cadherin 11 regulates MMP production and has important i

112 t corresponding to the putative shed form of cadherin-11 retains biological activity by promoting CNC

113 erent mammalian cell lines, while endogenous cadherin-11 shows focal adhesion localization in primary

114 Also, short hairpin RNA (shRNA) cadherin 11 silencing almost completely inhibited Cad-11

115 These data suggest that the presence of the cadherin-11 splice variant promotes invasion of cadherin

116 cell invasion, but the mechanisms underlying cadherin-11 stimulated cell migration are still incomple

117 s and other cell types that normally express cadherin-11, such as stromal cells or osteoblasts or per

118 Adhesion to fibronectin mediated by cadherin-11/syndecan-4 complexes requires both the extra

119 Cadherin-11 therapeutics prevent and reduce arthritis in

120 emonstrated colocalization of N-cadherin and cadherin 11 to the same points of cell-cell contact.

121 crest (CNC) cell migration both by cleaving cadherin-11 to release a promigratory extracellular frag

122 ated a 2-fold increased invasive capacity of cadherin 11-transfected L cells compared with L cells tr

123 Cadherin-11 was found to be expressed mainly in the syno

124 Recently, cadherin-11 was identified on fibroblast-like synoviocyt

125 One such potential biomarker, cadherin-11, was further evaluated at the protein level

126 Cadherin-8, as well as cadherin-11, was mapped to a specific region of chromoso

127 alpha, and a cellular adhesion protein gene, cadherin 11, were markedly regulated in response to diff

128 ownstream targets of CXCR7/RDC1 are CD44 and cadherin-11, which are likely to contribute to the invas

129 has a propensity to metastasize to bone, and cadherin-11, which is an osteoblast cadherin aberrantly

130 We now show that expression of wild-type cadherin-11, with or without coexpression of the COOH-te

131 of the specific combination of renal markers cadherin-11, WT-1, Pax-2, and Wnt-4.