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4 The activity assay results suggest that the caged-ADP may interact with PGK substrate binding site(s
5 le PGK activity decreased in the presence of caged-ADP, the activity was not influenced by the additi
6 ase of caged glutamate and a newly developed caged AEA (anandamide), together with rapid [Ca2+]i meas
7 ctivation was examined using photolysis of a caged agonist, carboxynitrobenzyl-tyrosine-[Leu(5)]-enke
8 Chemical tools for optopharmacology include caged agonists and antagonists and reversibly photoswitc
9 gh activation kinetics can be assessed using caged agonists, deactivation kinetics are obscured by sl
12 Carboxynitroveratryl-naloxone (CNV-NLX), a caged analog of the competitive opioid antagonist NLX, w
14 Opening of the coadded hairpin releases the caged analyte sequence, resulting in the dendritic RCA-i
16 Ru(II) center, the nitriles of complex 2 are caged, and 2 does not act as a potent enzyme inhibitor.
23 nm, optically selective uncaging of DEAC450-caged biomolecules at 900 nm may allow facile two-color
29 surface coordinated by the ribose 3'-OH and caged by arginine and lysine side chains is a putative m
35 duced by flash-induced Ca(2+) release from a caged Ca(2+) buffer), which we had reported previously.
41 otropic glutamate receptors or photolysis of caged Ca(2+) produced dilation of penetrating arterioles
42 ificial elevation of intracellular Ca(2+) by caged Ca(2+) release agents sensitizes hair cells to the
43 Accordingly, we applied flash photolysis of caged Ca(2+) to activate BK channels and determine their
44 uorometry and UV photolysis of intracellular caged Ca(2+), we optically resolved VSD activation promp
45 es of release, evoked by flash-photolysis of caged Ca(2+): exocytotic capacitance changes from indivi
49 ne capacitance together with photorelease of caged-Ca2+ and membrane depolarization to study exocytos
50 alized calcium transients with photolysis of caged calcium induced rapid outgrowth of axonal processe
52 d during activation in situ by photolysis of caged calcium using bifunctional fluorescent probes in t
54 used this chromophore to synthesize DEAC450-caged cAMP and found this probe was very stable toward a
57 these questions, we used flash photolysis of caged-cAMP (DMNB-cAMP) to provide high temporal resoluti
62 S release kinetics from a series of isomeric caged-carbonyl sulfide (COS) compounds, including thioca
66 d intracellular LIP following irradiation of caged-chelator-treated cells, but not in control samples
67 ntracellular Ca(2+) elevation through either caged chelators or pharmacological inhibitors of Ca(2+)
68 ecules loaded with a photocleavable peptide (caged Class II MHC molecules) enabled synchronous and ve
70 kinetics of opioid signaling we developed a caged competitive antagonist that can be quickly photore
71 of the pair of radicals forming the solvent-caged complex is caused by the reduced stability (high r
76 have recently utilized a new ruthenium-based caged compound, ruthenium-bipyridine-triphenylphosphine-
77 photolysis of many widely used nitroaromatic caged compounds (e.g., 4-carboxymethoxy-5,7-dinitroindol
79 mit UV light locally, for photoactivation of caged compounds and, in particular, used for photo-contr
85 ed to striatal cholinergic interneurons, the caged compounds were photolyzed in an chromatically orth
89 I describe important examples of widely used caged compounds, their design features and synthesis, as
95 oxymethyl ester); (ii) locally photolyzing a caged coumarin in one cell of a coupled cell pair to rel
96 s and photochemical properties of a coumarin-caged cyclic RGD peptide and demonstrate that uncaging c
97 ion, a conjugate between the dicyanocoumarin-caged cyclic RGD peptide and ruthenocene, which was sele
99 Saturation of transporters by photolysis of caged D-aspartate failed to elicit transporter currents
101 N-protected p-hydroxyphenacyl (pHP) ammonium caged derivatives at 313 nm releases primary and seconda
102 is- (photo-DIBOD, 1) and mono-cyclopropenone-caged dibenzocyclooctadiynes (MC-DIBOD, 5) allows for se
104 verage number of cooperative duplexes, these caged dimers constitute the first example of cooperative
105 ly route to a variety of discrete cyclic and caged disulfide structures, which can then be kineticall
107 ntisense agents through the preparation of a caged DNA phosphoramidite and its site-specific incorpor
108 xed but rather maintained within ellipsoidal caged domains, similar to eukaryotic interphase chromoso
111 tion in vivo, we developed a bioconjugate of caged dye, named dextran-CANPE-HCC, for imaging cell cou
118 tumor cell line) were microinjected with the caged FAK peptide and locally photoactivated using a foc
122 the separation capillary by photolysis of a caged-fluorescein label using the 351-364 nm irradiation
124 ZE) based upon photophysical activation of a caged, fluorogenic label covalently attached to the targ
126 s tetrazine reactivity to unmask vinyl ether caged fluorophores spanning the visible spectrum, includ
127 espread used indicator of live bacteria is a caged form of carboxyfluorescein in which 3' and 6' hydr
128 Here we report that photolysis of a double-caged form of the second messenger inositol 1,4,5-trisph
130 izontal lineNOR' are of interest as prodrug (caged) forms of the bioeffectors nitric oxide (NO) and n
132 d application of a practically useful doubly caged GABA analog, termed bis-alpha-carboxy-2-nitrobenzy
133 njugation of a large, neutral dendrimer to a caged GABA probe we introduce a "cloaking" technology th
134 ght penetration from an LED and diffusion of caged GABA to quickly terminate intense focal seizures.
137 trifluoromethylated p-hydroxyphenacyl (pHP)-caged gamma-aminobutyric acid (GABA) and glutamate (Glu)
138 ysiological recording, photolytic release of caged glutamate and a newly developed caged AEA (anandam
139 also used laser scanning photostimulation of caged glutamate and whole cell recordings to map excitat
141 o acid carrier 1) through photo-release from caged glutamate generated a transient inward current, as
142 ed laser scanning photostimulation (LSPS) of caged glutamate in conjunction with whole-cell patch-cla
143 the preBotC using holographic photolysis of caged glutamate in medullary slices from neonatal mice.
144 nation with minimal and maximal stimulation, caged glutamate photolysis, and single axon tracing.
145 we used laser-scanning photostimulation with caged glutamate to characterize the spatial distribution
146 l apical dendrites using focal photolysis of caged glutamate triggered abnormally prolonged plateau p
147 d), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal memb
148 e were stimulated by using the photolysis of caged glutamate, and in vitro whole-cell patch-clamp rec
149 -photon calcium imaging, flash photolysis of caged glutamate, and patch-clamp electrophysiology in co
150 ial CSF (23 degrees C) containing 100 microM caged glutamate, APV (2-amino-5-phosphonovaleric acid),
151 Here, using fast 3D two-photon imaging and a caged glutamate, we challenge this classical view by dem
154 recordings, and scanning photostimulation of caged glutamate, we report first that principal neurons
159 ental results demonstrated that neither DECM-caged glycine nor its byproduct inhibits or activates hu
162 PgammaS) in solution, and caged GTPgammaS or caged GTP loaded on the RhoA.RhoGDI complex to show that
163 iotriphosphate) (GTPgammaS) in solution, and caged GTPgammaS or caged GTP loaded on the RhoA.RhoGDI c
164 We used photolysis of caged phenylephrine, caged guanosine 5'-O-(thiotriphosphate) (GTPgammaS) in s
165 ate a fluorescence response and also release caged H2S, thus addressing challenges of analyte homeost
167 ional alterations of eight selected genes in caged honey bees exposed to different concentrations of
168 n (Kcat and kcat/Km), DFP decay, and protein-caged hydrated ferric oxide accumulation decreased in fe
169 drug discovery, and entertains the classical caged hydrocarbon systems potentially missing from the c
172 ion of fathead minnows (Pimephales promelas) caged in four Nebraska, USA watersheds - the Niobrara an
173 osphotriesterase (a bioremediation catalyst) caged in GSBs and isolate a 20-fold faster mutant in les
174 tem consists of the analyte-sequence that is caged in the stem region and a single-stranded loop doma
175 benzyl trimethylammonium hydroxide (PVBTAH) caged in ZIF-8 is synthesized in steps of chloro-monomer
176 NAzyme) linked to a G-rich domain, which is "caged" in the complex structure, is assembled on Au-coat
178 d one-fourth of the HRP-mimicking DNAzyme in caged, inactive configurations are used as functional el
182 ormer describes the organic synthesis of the caged IP(3), which requires 12 d, and the latter an appl
188 energy with higher cooperativity for double-caged IP3 than for conventional single-caged IP3, consis
189 isphosphate (IP3) receptors by photolysis of caged IP3 The rate of Ca(2+) removal from the cytosol wa
190 ouble-caged IP3 than for conventional single-caged IP3, consistent with a chemical two-photon effect.
191 PIP2 was inhibited by the addition of single-caged IP3, suggesting that single-caged IP3 is an antago
194 siologically relevant doses of UVA, such as "caged-iron chelators", may provide dose- and context-dep
196 Here, a new lanthanide-chelating NMR probe, Caged Lanthanide NMR Probe-7 (CLaNP-7), is presented.
198 , and in vivo imaging applications of Copper-Caged Luciferin-1 (CCL-1), a bioluminescent reporter for
199 t the synthesis and characterization of iron-caged luciferin-1 (ICL-1), a bioluminescent probe that e
200 ynthesis, and in vivo applications of Peroxy Caged Luciferin-1 (PCL-1), a chemoselective bioluminesce
201 lish this approach, we have developed Peroxy Caged Luciferin-2 (PCL-2), a H(2)O(2)-responsive boronic
202 sualizing cellular networks, we developed a "caged" luciferin that produces light only when cells are
207 Here we describe a strategy for the use of a caged metabolic precursor that is activated for cellular
208 HC products loaded with conditional ligands (caged MHC molecules) provides a fast and straightforward
209 ational change that locks the substrate in a caged Michaelis complex that provides optimal stabilizat
210 ianion activation by stabilization of active caged Michaelis complexes may be generalized to the many
212 manipulations, as exemplified by the use of caged morpholino (cMO) oligonucleotides to inactivate ge
214 d antagonist, carboxynitroveratryl-naloxone (caged naloxone), blocked the current induced by a series
219 Thus, the synthesis of dinitroindolinyl-caged neurotransmitters is within the scope of a modestl
220 ray of strategies, including photorelease of caged neurotransmitters, engineered light-gated receptor
223 ng two-photon activation of an intracellular caged NMDA receptor antagonist (tc-MK801), we found that
224 oscopy and laser-triggered NO release from a caged NO compound, we found that both free hemoglobin an
228 present investigation used photolysis of two caged opioid ligands to examine the kinetics of MOR-indu
231 lculations, grows rapidly with the number of caged p-H(2) molecules and is a significant fraction of
234 For both methods and for all the radical caged pairs investigated, there were no observable heavy
235 n can be efficiently performed both with the caged peptide and with its ruthenocenoyl bioconjugate re
236 farnesyltransferase; irradiation of the NDBF-caged peptide in the presence of the enzyme resulted in
238 onjugate reveals great potential for DEAdcCE-caged peptide sequences as selective drug carriers in th
241 c force decay upon release of phosphate from caged phosphate was previously interpreted as a signatur
245 7-(diethylamino)coumarin-4-yl]methyl (DEACM)-caged phosphorylated serine, threonine, and tyrosine bui
247 ing 'click' chemistry to couple biotin to a 'caged' photocleavable (PC) guanosine monophosphate (GMP)
249 3-3%) suggests that photolysis of 200 microm caged precursor is sufficient for full PKA activation.
251 of firefly luciferin from two complementary caged precursors that can be unmasked by different bioch
252 e been extended to allow the introduction of caged proteins into cells by permeabilization or microin
253 ver, the chemical or genetic construction of caged proteins is nontrivial, with subsequent laborious
257 o muscle contraction and that the release of caged protons is sufficient to induce muscle contraction
258 c and protein-based photoactivatable probes, caged QDs can confer increased spatial and temporal reso
259 ovel optical properties, we have synthesized caged quantum dots, which are nonluminescent under typic
261 O proceed through the same [Fe(IV)=O *NO(2)] caged radical intermediate and lead to similar outcomes.
265 II directly or through photo-release of the caged Rho kinase inhibitor also reduced the rate of VE-c
266 st bioadhesion, were internally labeled with caged rhodamine to make the particles photoactivatable.
268 ferent approaches were investigated with the caged RNA molecules: the light-regulation of catalytic R
270 d a synthetic approach to the preparation of caged secondary amines by acid-catalyzed rearrangement o
271 transmission model, in which guinea pigs are caged separately, the oseltamivir-resistant viruses tran
274 us elevation of intracellular Sph levels via caged Sph leads to a significant and transient calcium r
277 line-earth complexes prefer a highly compact caged structure with both phenyl rings providing cation-
281 exemplify the utility of this amino acid, we caged the nuclear localization sequences (NLSs) of nucle
282 orpholino-based antisense reagents have been caged through oligonucleotide cyclization, enabling phot
283 e was developed through the incorporation of caged thymidine nucleotides into a DNA-based logic gate.
287 w (Pimephales promelas), a small-bodied fish caged upstream and downstream of a local wastewater trea
288 combination with 1- or 2-photon excitation, caged vanilloids are a powerful tool for probing morphol
289 oton excitation with red light (720 nm), the caged vanilloids can be photoreleased in situ to activat
291 synthesis and biological application of two caged vanilloids: biologically inert precursors that, wh
292 ovarian carcinoma (SKOV3) cells with an NDBF-caged version of a farnesylated peptide followed by UV i
294 ter male mosquitoes can efficiently suppress caged wild-type mosquito populations, providing the foun
296 /6J female mice were randomly assigned to be caged with an unlocked (U) or locked (L) running wheel b
298 of alcohols, phenols, and carboxylic acids "caged" with the (3-hydroxy-2-naphthalenyl)methyl group r
299 of alcohols, phenols, and carboxylic acids "caged" with the 2,5-dihydroxybenzyl group or its naphtha
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