ライフサイエンスコーパス: capacitation

1 m to acquire acrosomal responsiveness during capacitation.

2 se sperm and is coupled to events regulating capacitation.

3 They also did not undergo proper capacitation.

4 rylation events that take place during sperm capacitation.

5 osine phosphorylation events associated with capacitation.

6 annels in epididymal sperm examined prior to capacitation.

7 protein tyrosine phosphorylation as well as capacitation.

8 in tyrosine phosphorylation that accompanies capacitation.

9 ia to support signal transduction leading to capacitation.

10 ated rabbit spermatozoa and is unaffected by capacitation.

11 not occur in a medium that does not support capacitation.

12 ed in the female tract in a process known as capacitation.

13 mal tyrosine phosphorylation associated with capacitation.

14 These are themselves shed from sperm during capacitation.

15 city in the female tract in a process called capacitation.

16 terol efflux, an important step during sperm capacitation.

17 otility and hyperactivation typical of sperm capacitation.

18 otein tyrosine phosphorylation of late-stage capacitation.

19 ime-dependent effects on multiple aspects of capacitation.

20 otein that is tyrosine-phosphorylated during capacitation.

21 ding reverse cholesterol transport and sperm capacitation.

22 (P<0.0001) before and, more severely, after capacitation.

23 ll as serine/threonine phosphorylated during capacitation.

24 ter leaflet of null spermatozoa before sperm capacitation.

25 co-factor-independence of GalT I-null sperm capacitation.

26 is protein is tyrosine phosphorylated during capacitation.

27 ify phosphorylation changes occurring during capacitation.

28 the protein phosphorylation cascade of sperm capacitation.

29 oes tyrosine phosphorylation during in vitro capacitation.

30 e found to be tyrosine phosphorylated during capacitation.

31 mally required by wild-type sperm to achieve capacitation.

32 tion can occur, mammalian sperm must undergo capacitation, a process that requires a cyclic AMP-depen

33 reduced ability to fertilize eggs, although capacitation and acrosome exocytosis appear to be normal

34 of eIF2alpha phosphorylation delays ribosome capacitation and favors reinitiation at ATF4 over the in

35 Events in sperm capacitation and fertilization require similar cellular

36 naling pathways regulating such processes as capacitation and flagellar motility.

37 This maturational process is called capacitation and in mouse sperm it involves a plasma mem

38 ntral role in the control of mammalian sperm capacitation and motility.

39 the Ca(2+) store, which may be regulated by capacitation and NO from the cumulus.

40 ouse sperm with Ca(2+) ionophore accelerated capacitation and rescued fertilizing capacity in sperm w

41 ated to play roles in spermatogenesis, sperm capacitation and sperm-egg binding in mammals.

42 Ca(2+) regulate sperm motility, chemotaxis, capacitation and the acrosome reaction, and play a vital

43 n cascade(s) regulating events pertaining to capacitation and/or motility in mammalian sperm and that

44 and voltage, Slo3 could be involved in sperm capacitation and/or the acrosome reaction, essential ste

45 tes, histamine secretion by basophils, sperm capacitation, and airway pH.

46 didymal sperm count, spermatozoa morphology, capacitation, and motility and reduced ejaculated sperm

47 ortance, the molecular mechanisms underlying capacitation are poorly understood.

48 ism attenuates alkaline shifts in pHi during capacitation as well as the ability of sperm to produce

49 HDL also supports sperm capacitation, as assessed by fertilization in vitro.

50 )/Cl(-) cotransporters (NKCC), inhibited all capacitation-associated events, suggesting that these tr

51 monstrated that HCO-(3) is necessary for the capacitation-associated increase in protein tyrosine pho

52 olesterol binding protein, also supports the capacitation-associated increase in protein tyrosine pho

53 protein kinase A (PK-A) is upstream of this capacitation-associated increase in protein tyrosine pho

54 nt of Na(+) by choline(+) also inhibited the capacitation-associated increase in protein tyrosine pho

55 r to stallion and human, PF431396 blocks the capacitation-associated increase in tyrosine phosphoryla

56 c kinase family inhibitor SU6656 blocked the capacitation-associated increase in tyrosine phosphoryla

57 Recently, it has been proposed that the capacitation-associated increase in tyrosine phosphoryla

58 activating mutation in Fer failed to undergo capacitation-associated increases in tyrosine phosphoryl

59 However, the block of capacitation-associated parameters was overcome when spe

60 n the other hand, in conditions that support capacitation-associated processes blocking hyperpolariza

61 In this experimental setting, other capacitation-associated processes such as activation of

62 of Cl(-) did not affect sperm viability, but capacitation-associated processes such as the increase i

63 Both the capacitation-associated tyrosine phosphorylation and the

64 t undergo a physiological maturation, termed capacitation, before they are able to fertilize eggs.

65 factors from wild-type sperm phenocopies the capacitation behavior of GalT I-null sperm.

66 ltering sperm functions, including motility, capacitation, binding to the zona pellucida, binding to

67 in wild-type sperm was maximal after 2 h of capacitation, but capacitation of sperm from spasmodic m

68 roduce capacitation in vitro or induction of capacitation by cell-permeant cAMP analogs decreased the

69 s sperm motility, an important early step in capacitation, by increasing flagellar beat frequency thr

70 icipates in signaling pathways that regulate capacitation, Cl(-) was replaced by either methanesulfon

71 The molecular basis of mammalian sperm capacitation, defined as those biochemical and functiona

72 Signaling events leading to mammalian sperm capacitation depend on the modulation of proteins by pho

73 HCO(3)(-) current and the inhibition of the capacitation-dependent increase in protein tyrosine phos

74 Pmca4-/- sperm that had not undergone capacitation exhibited normal motility but could not ach

75 These two behavioral consequences of capacitation, exocytotic competence and altered motility

76 ulating protein tyrosine phosphorylation and capacitation, further supports the importance of PK-A in

77 In spermatozoa, capacitation, hyperactivation of motility and the acroso

78 As expected, wild-type sperm must undergo capacitation in order to bind the zona pellucida and und

79 lT I may function as a negative regulator of capacitation in the sperm head by suppressing intracellu

80 Sperm capacitation in vitro is highly correlated with an incre

81 Incubations known to produce capacitation in vitro or induction of capacitation by ce

82 nduced acrosome reactions without undergoing capacitation in vitro.

83 ertilize an oocyte, sperm must first undergo capacitation in which the sperm plasma membrane becomes

84 Considering that capacitation-induced hyperpolarization is mediated by SL

85 d that inhibition of its activity blocks the capacitation-induced hyperpolarization of the sperm plas

86 Slo3 is the main current responsible for the capacitation-induced hyperpolarization, which is require

87 sine phosphorylation that is associated with capacitation, induction of the acrosome reaction, forwar

88 We previously demonstrated that mouse sperm capacitation is accompanied by a time-dependent increase

89 Capacitation is also accompanied by hyperpolarization of

90 erm capacitation." We have demonstrated that capacitation is associated with an increase in the tyros

91 In addition, mouse sperm capacitation is associated with the hyperpolarization of

92 This inhibitor revealed that capacitation is defined by separable events: induction o

93 Capacitation is needed for the activation of motility (e

94 tly, this work presents strong evidence that capacitation is regulated by two parallel pathways.

95 Sperm capacitation is required for fertilization.

96 data suggest that one aspect of mouse sperm capacitation is the selective activation of one major pH

97 also increased the percentage of sperm with capacitation-like changes in membrane structure.

98 In a process called capacitation, mammalian sperm gain the ability to fertil

99 t increases in cAMP content occurring during capacitation may inhibit ENaCs to produce a required hyp

100 Cl(-) transport, sperm incubated in complete capacitation medium were exposed to a battery of anion t

101 We demonstrate in this report that capacitation of cauda epididymal mouse sperm in vitro wa

102 Interestingly, capacitation of GalT I-null sperm is independent of the

103 ning the requirement for PKA activity during capacitation of sperm from mice that express CalphaM120A

104 m was maximal after 2 h of capacitation, but capacitation of sperm from spasmodic mice for up to 3 h

105 els of eIF2alpha phosphorylation favor early capacitation of such reinitiating ribosomes directing th

106 hyperactivated motility, which occur late in capacitation of wild-type spermatozoa, do not develop in

107 shed by their morphology, ability to undergo capacitation or the acrosome reaction, and/or mitochondr

108 In this paper, we describe the capacitation phenotype of sperm lacking the long isoform

109 When sperm were subjected to 'in vitro' capacitation, photo-stimulation also increased the perce

110 The latter beat pattern is part of the capacitation process whereby sperm prepare for the prosp

111 sperm were incubated in a medium supporting capacitation, proteins became tyrosine-phosphorylated in

112 Signaling events leading to mammalian sperm capacitation rely on activation/deactivation of proteins

113 It is well established that capacitation requires Na(+), HCO(3)(-), Ca(2+), and a ch

114 At the molecular level, capacitation requires protein kinase A activation, chang

115 vation of this signaling pathway, as well as capacitation, requires bovine serum albumin (BSA) in the

116 Furthermore, these sperm show accelerated capacitation resulting in an overall in vitro fertilizin

117 revents engagement of multiple components of capacitation resulting in male infertility.

118 Sperm capacitation results in pHi increases (from 6.54 +/- 0.0

119 Sperm capacitation selectively exposed a partial von Willebran

120 During sperm capacitation SLO3 hyperpolarizes the sperm, whereas CATS

121 During capacitation, sperm lose a fraction of their Sias.

122 rgism between S1 and S2 for abnormalities in capacitation, sperm-oolemma binding, and zona-free oocyt

123 However, after capacitation, staining in the neck decreased, and most o

124 its increased phosphorylation status during capacitation suggested multiple important functions for

125 cal localization did not change during sperm capacitation, suggesting that glycolysis in sperm is reg

126 daptations within the female (the process of capacitation) that are initiated by agents ranging from

127 During capacitation the mouse sperm plasma membrane potential (

128 However, one of the component processes of capacitation, the ability to undergo a zona pellucida-ev

129 to play a pivotal role in sperm motility and capacitation, the distinctive biochemical properties of

130 signaling events leading to mammalian sperm capacitation, the immediate activation of protein kinase

131 In contrast, a second component process of capacitation, the transition to hyperactivated flagellar

132 During this process of capacitation, they acquire progressive motility, develop

133 Balpha system can regulate cytokine receptor capacitation through effects on the induction of downstr

134 BSA is hypothesized to modulate capacitation through its ability to remove cholesterol f

135 BSA is hypothesized to modulate capacitation through the removal of cholesterol from the

136 required for at least the initial 30 min of capacitation to produce subsequent protein tyrosine phos

137 of AE in which membrane fusions occur during capacitation/transit through the cumulus, prior to any p

138 To identify proteins phosphorylated during capacitation, two-dimensional gel analysis coupled to an

139 , the increase in PK-A activity accompanying capacitation was associated with enzyme activity found i

140 at glucose is not essential for murine sperm capacitation, we demonstrated that glucose (but not lact

141 ertilize constitute the phenomenon of "sperm capacitation." We have demonstrated that capacitation is

142 After epididymal maturation, sperm capacitation, which encompasses a complex series of mole