ライフサイエンスコーパス: capsid

1 ration to prevent assembly of a mature viral capsid.

2 s and joins 89 coat protein dimers to form a capsid.

3 to studying the chemical details of the HIV capsid.

4 isrupted and that this could destabilize the capsid.

5 and at the Cyclophilin A binding loop of the capsid.

6 oteins, which result in the expansion of the capsid.

7 l lipid membrane enclosed in the icosahedral capsid.

8 (HIV-1) infection is highly dependent on its capsid.

9 c and heterotypic interactions to create the capsid.

10 interacts directly with viral DNA within the capsid.

11 s the extra-swelling capacity of these T = 1 capsids.

12 ng RNA-2, and of naturally-formed empty CPMV capsids.

13 ranging from self-assembling cages to viral capsids.

14 ns assembled into tubes or sheets instead of capsids.

15 tion and utilization of structurally related capsids.

16 for the intrinsic chirality observed in all capsids.

17 ys through which a viromimetic protein forms capsids.

18 plying this paradigm to a dodecahedral viral capsid, a computer-derived nucleotide sequence is evolve

19 The viral capsid, a conical shell encasing the viral ribonucleopro

20 he 3.3-A map of the mature P22 bacteriophage capsid, a large and complex macromolecular assembly.

21 nit length genomes and translocate them into capsids, a critical process in the production of infecti

22 Transmission electron microscopy revealed capsids accumulated at nuclear pores that retained the v

23 Thus, capsids adopt conformations that undergo rapid disassemb

24 ermal growth factor to genome-free MS2 viral capsids, affording nanoscale delivery vectors that can t

25 In this study, the HTLV-1 capsid amino-terminal domain was found to provide distin

26 a global conformational rearrangement of the capsid and a complex alteration of its equilibrium dynam

27 Packaging of the genome into a protein capsid and its subsequent delivery into a host cell are

28 +) T cells were focused predominantly on the capsid and nonstructural NS3 and NS5 antigens.

29 of self-assembly and disassembly of the HIV capsid and protein-based two-dimensional nanomaterials a

30 The capsid and replicase proteins of PCV3 are only 37% and 5

31 th that also couples to a model of the viral capsid and tail tube.

32 ller size because the tegument layer between capsid and viral envelope was reduced.

33 ects in envelopment, there was missorting of capsids and enveloped particles in the neuronal cytoplas

34 reduced anterograde transport of unenveloped capsids and enveloped virions.

35 c factor IX cassette into haploid AAV2/8 1:3 capsids and injected them into FIX knockout mice via the

36 ell spread and produced fewer DNA-containing capsids and more empty capsids than wild-type virus.

37 atios (3:1, 1:1 and 1:3) to assemble haploid capsids and study both their transduction efficiency and

38 gnatures for the hexon, penton, fiber, minor capsid, and core proteins.

39 p gene, which encodes proteins that form the capsid, and the right-hand inverted terminal repeat.

40 bly of AAV4, -5, and -11, and AAP, assembled capsids, and the nucleolus do not colocalize for all the

41 o the nucleolar enrichment of assembled AAV2 capsids; and, surprisingly, AAV4, -5, and -11 capsids ar

42 vasion of Nab recognition without increasing capsid antigen presentation in target cells.

43 hrough detection of viral RNAs by RT-PCR and capsid antigens by immunostaining methods.

44 Viral capsids are a prototypical example, where coat proteins

45 iffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site

46 The capsids are decorated by large spikes around the 5-fold

47 -5, -11, and -12; assembled AAV5, -8, and -9 capsids are excluded from the nucleolus, in contrast to

48 Many virus capsids are icosahedral, and assembly is thought to occu

49 Icosahedral viral capsids are made of a large number of symmetrically orga

50 apsids; and, surprisingly, AAV4, -5, and -11 capsids are not dependent on AAP for assembly.

51 none from nonstructural proteins, indicating capsids are packaged as cargo into eHAV vesicles via a h

52 double-stranded DNA is tightly packed in the capsid as a left-handed superhelix and held in place by

53 maging, we demonstrate that these artificial capsids assemble as 20-nm hollow shells that attack bact

54 escribed, the mechanism by which these large capsids assemble remains enigmatic.

55 entry, genome replication, gene expression, capsid assembly and cytoplasmic envelopment, and transce

56 associated virus (AAV) protein that promotes capsid assembly and provides new opportunities for resea

57 Understanding capsid assembly is important because of its role in viru

58 ese findings suggest that both PGC1alpha and capsid assembly may represent attractive targets for the

59 nt core proteins was susceptible to multiple capsid assembly modulators.

60 that AAP is not an essential requirement for capsid assembly of AAV4, -5, and -11, and AAP, assembled

61 olocalize; AAPs are promiscuous in promoting capsid assembly of other serotypes, with the exception o

62 angles, which may reflect differences in the capsid assembly pathway for these viruses.

63 the serotype-dependent heterogeneity of the capsid assembly process and challenge current notions ab

64 show that biological properties of AAPs and capsid assembly processes are surprisingly distinct amon

65 ectively) and examined the AAP dependence of capsid assembly processes of these 12 serotypes using co

66 erapy; however, fundamental aspects of AAV's capsid assembly remain poorly characterized.

67 s a new pharmacological vulnerability in the capsid assembly stage of the HIV-1 life cycle.IMPORTANCE

68 We uncover the complex multistage process of capsid assembly, which involves recruitment and complexa

69 bstitution, Pro272Lys, significantly reduced capsid assembly, while a Cys273Ser change appeared to al

70 s about the role of AAP and the nucleolus in capsid assembly.

71 ur study revealed how adenoviruses exploit a capsid-associated small PPxY peptide motif to manipulate

72 d movement in these viruses is controlled by capsid-associated tegument proteins, yet their specific

73 he capsid into the cytoplasm, docking of the capsid at a nuclear pore, and release of the viral genom

74 yze the dynamics of the minute virus of mice capsid at increasing temperatures.

75 stage(s) beyond entry, after the arrival of capsids at the nucleus.

76 time the assembly of Hepatitis B Virus (HBV) capsids below the pseudocritical concentration.

77 Pb10 consists of an alpha-helical capsid-binding domain and an Ig-like domain exposed to t

78 nformational exchange events observed in the capsid-binding domain enable rearrangements upon binding

79 the strength of the capsid structure and the capsid breaks open.

80 are exposed to disinfection that targets the capsid, but less so when the virus genome is directly ta

81 e energy barrier through mild heating of the capsid, but little is known about the capsid regions inv

82 ility mapped to the HIV-2 envelope (Env) and capsid (CA).

83 Capsids, carboxysomes, exosomes, vacuoles and other nano

84 st sex pilus and mechanisms underlying ssRNA-capsid co-assembly, and inspires speculation about the l

85 nt with the previously proposed dsDNA genome-capsid coassembly for adenoviruses, which resembles that

86 and supports the previously proposed genome-capsid coassembly mechanism for adenoviruses.

87 lding that would be occurring during the RNA/capsid coassembly process.

88 e observed a structural rearrangement in the capsid coat proteins that is required to package the vir

89 e nucleolus and is dependent on AAP and that capsids colocalize with AAP in the nucleolus during the

90 g of both vRNPs and IN within the protective capsid cores to facilitate subsequent reverse transcript

91 y at the expense of modularity, making viral capsids difficult to engineer.

92 oscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization o

93 ipheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels cap

94 n the core, which triggers the initiation of capsid disassembly.IMPORTANCE For successful infection,

95 of nucleolar association; AAPs and assembled capsids do not necessarily colocalize; AAPs are promiscu

96 required for releasing the viral genome from capsids docked at nuclear pores.

97 nterferes with assembly of the conical viral capsid during virion maturation and results in perturbat

98 rticle (VLP) vaccines that mimic the "empty" capsids (ECs) normally produced in viral infection.

99 A key knowledge gap is how the capsid engages the NPC and what triggers release of the

100 sm of pumping dsDNA into a preformed protein capsid exemplified by tailed bacteriophages and herpesvi

101 Applying a capsid flow cytometry assay, we identified two 2'-C-meth

102 ficant role of microtubules in the efficient capsid formation during HBV replication.

103 bule and thus a high capacity to support the capsid formation.

104 assembly agents, resulting in attenuation of capsid formation.

105 ious studies have shown the compatibility of capsids from AAV serotypes and homology of recognition s

106 ver, we showed that the assembly of chimeric capsids from wild-type and drug-resistant core proteins

107 , which in part reflects the requirement for capsid function at both the efferent and afferent phases

108 processing of this pre-mRNA and so controls capsid gene access via its role in alternative internal

109 Thus, in addition to controlling capsid gene access, NP1 also controls the expression of

110 However, the universally conserved minor capsid gene vp3 could be deleted without a loss in infec

111 ses, including typing of both polymerase and capsid genes, is important for monitoring emerging strai

112 A-diameter isometric mutant bacteriophage T4 capsid has been determined.

113 ion and overall shape of several giant virus capsids have been described, the mechanism by which thes

114 Fluorescent protein fusions to herpesvirus capsids have proven to be a valuable method to study vir

115 hts from the nuclear assembly of large viral capsids highlight a back door route for nuclear escape,

116 Comprehensive understanding of capsid-host interactions that promote or impede HIV-1 in

117 ed that a P73G mutation that lies inside the capsid immediately adjacent to a putative zinc binding s

118 l role in releasing viral DNA from NPC-bound capsids.IMPORTANCE Herpes simplex virus 1 (HSV-1) is the

119 ssembly of the T = 4 hepatitis B virus (HBV) capsid in real time.

120 ities of unenveloped and partially enveloped capsids in neuronal cytoplasm.

121 had greater proportions of empty (DNA-less) capsids in the cytoplasm of infected cells, suggesting t

122 ation of biologically aberrant multi-layered capsids in the virion.

123 to double-stranded DNA and released from the capsid (in a process known as uncoating) before it can b

124 is shared between two or more 22-nm-diameter capsids, in analogy with the multiplets of 28-nm-diamete

125 000 nt in length are packaged into multiplet capsids, in which a single RNA molecule is shared betwee

126 everse transcription the pressure inside the capsid increases until the internal stress exceeds the s

127 everse transcription the pressure inside the capsid increases, reaching a maximum after 7 h.

128 accine (mOPV1) and subsequently treated with capsid inhibitor pocapavir or placebo.

129 ute to the quasi-irreversibility of the pb10-capsid interaction.

130 This atomic description of genome-capsid interactions in a spherical ssRNA virus provides

131 ecent work has begun to reveal how the viral capsid interacts with specific cellular proteins to prom

132 to the rapid release of the genome once the capsid interacts with the nuclear pore.

133 of SAT2 viruses by mutating residues at the capsid interface through predictive modeling.

134 sities extending the 5-fold channel into the capsid interior are conserved among the bocaparvoviruses

135 ly steps in infection include release of the capsid into the cytoplasm, docking of the capsid at a nu

136 AV egress involving endosomal budding of HAV capsids into multivesicular bodies.

137 The capsid is a large container, made of approximately 1,300

138 Unlike related mimiviruses, the CroV capsid is not decorated with glycosylated surface fibers

139 The reovirus outer capsid is stabilized by mu1 intratrimer, intertrimer, an

140 de evidence that antibody recognition of AAV capsids is conserved across species.

141 iral RNA genome and IN within the protective capsid lattice to ensure subsequent reverse transcriptio

142 NPs) are eccentrically localized outside the capsid lattice.

143 eccentrically localized outside the conical capsid lattice.

144 emains active and stably associated with the capsid lattice.

145 a specific protein-protein interface in the capsid lattice.

146 culon-like transmembrane protein A17 and the capsid-like scaffold protein D13 was sufficient to form

147 l (AAV) vector consisting of a bioengineered capsid, liver-specific promoter and factor IX Padua (fac

148 cies that, in contrast to CAdV, has a unique capsid morphology that contains more prominent extension

149 Capsid movement in these viruses is controlled by capsid

150 ealed that C3P3 enhanced processing of outer capsid mu1 protein to mu1C, a previously described hallm

151 Our data show that some capsid mutations stabilize the ECs and could be applied

152 Here, we present a reconstruction of the capsid of Cafeteria roenbergensis virus (CroV), one of t

153 In the capsid of the minute virus of mice, events required for

154 mes by packaging them into an outer shell or capsid of virus-encoded proteins.

155 mes by packaging them into an outer shell or capsid of virus-encoded proteins.

156 cryo-EM reconstruction, we propose that the capsids of CroV and related giant viruses are assembled

157 The capsids of SBPV virions in low pH are not expanded.

158 Viruses build icosahedral capsids of specific size and shape by regulating the spa

159 s achieved through covalent linkage of phage capsid onto the carbon nanotubes.

160 l lineage, bacteriophage PM2, extends to the capsid organization (pseudo T = 21 dextro) despite the d

161 at involve translocation of peptides through capsid pores are associated with a subtle conformational

162 Thus, the translocation events through capsid pores involve a global conformational rearrangeme

163 A synthetic capsid-premembrane-envelope (C-prM-E) gene construct of

164 is, it mediates a dramatic increase in viral capsid production and robust viral replication.

165 ng inhibitors that block recognition of VEEV capsid protein (C) by the host importin (IMP) alpha/beta

166 The orthoretroviral capsid protein (CA) assembles into polymorphic capsids,

167 s study, the hexagonal lattice formed by the capsid protein (CA) of human immunodeficiency virus (HIV

168 lding protein (SP) for a binding site on the capsid protein (CP), and works by altering the angle bet

169 he latter system, nucleolar presence of ZIKV capsid protein (ZIKV-C) was associated with ribosomal st

170 crystal structure of a complex of the HAstV capsid protein and a virus-neutralizing antibody.

171 rus (KSHV) mutant that is defective in small capsid protein and is unable to produce mature virions.

172 es, without affecting the integrity of viral capsid protein and the viral particle.

173 u hybridization were used to determine viral capsid protein and viral DNA respectively.

174 adenovirus lineage, characterized by a major capsid protein bearing two beta-barrels.

175 CD8(+) T-cell responses against the AAV capsid protein can, however, affect therapeutic efficacy

176 These results demonstrate that the pUL25 capsid protein has a critical role in releasing viral DN

177 of the HIV-1 life cycle.IMPORTANCE The HIV-1 capsid protein is an attractive but unexploited target f

178 human immunodeficiency virus type 1 (HIV-1) capsid protein is an attractive therapeutic target, owin

179 Most, but not all, of the capsid protein is rapidly shed in tissue culture and pri

180 t in a dissociation of a subset of the major capsid protein L1 from the minor capsid protein L2, whic

181 particle (VLP) formed by the self-assembling capsid protein L1.

182 f the major capsid protein L1 from the minor capsid protein L2, which remains in complex with the vir

183 Moreover, the major capsid protein locus of pacmanvirus appears to be differ

184 ts suggest that the P17 protein is the minor capsid protein of Bam35 and P24 is the penton protein, w

185 hibiting the final protease cleavage between capsid protein p24 and spacer protein-1, producing immat

186 with HIV viral replication, assayed by virus capsid protein p24 production.

187 uclear pore complex (NPC) is mediated by the capsid protein pUL25 and the capsid-tethered tegument pr

188 -1 isolates bearing defined mutations in the capsid protein revealed differences in their cytoplasmic

189 We determined the crystal structure of the capsid protein spike domain from one of these HAstV stra

190 ought to occur by the sequential addition of capsid protein subunits to a nucleus, with the final ste

191 identify and characterize a mutation in the capsid protein that confers resistance to the inhibitor.

192 motif in the exposed, antigenic, GH loop of capsid protein VP1.

193 otein fusions to the amino terminus of small capsid protein VP26 are the most widely used method to v

194 The C-terminal extension of capsid protein VP3 folds into a globular protruding (P)

195 HHs bind to a site on the top surface of the capsid protein VP3, which is hidden in the native virus.

196 However, the structure of the viral major capsid protein, elucidated at near-atomic resolution usi

197 into the host cell by retention of the minor capsid protein, L2, and the viral genome instead of traf

198 protein complex, along with its constitutive capsid protein, plays essential roles at virtually every

199 s) produced by recombinant expression of the capsid protein, using cryogenic electron microscopy.

200 ic promoter at levels similar to that of the capsid protein-coding mRNA and is essential for replicat

201 usions that are formed by loops of the major capsid protein.

202 e of the infectious particle-associated ORF2 capsid protein.

203 severity, is a soluble variant of the viral capsid protein.

204 m patients, HEV produced 3 forms of the ORF2 capsid protein: infectious/intracellular ORF2 (ORF2i), g

205 Multiple peptides were identified from HAV capsid proteins (53.7% coverage), but none from nonstruc

206 ificially tethering viral mRNAs encoding Gag capsid proteins (gag-pol mRNAs) to distinct non-PM subce

207 igner" AAV, AAV2/Anc80L65, in which the main capsid proteins approximate the ancestral sequence state

208 Although the capsid proteins are all identical, they nevertheless arr

209 , indicating that they are still composed of capsid proteins arranged in a hexagonal lattice.

210 studies have revealed that herpesvirus small capsid proteins bind to capsids via their amino terminus

211 ture consisting of the herpesvirus-conserved capsid proteins MCP, Tri1, Tri2, and SCP and the HCMV-sp

212 gets nonassembled and virus particle-forming capsid proteins to mediate their autophagy-dependent deg

213 A limit of detection of 0.2 ng/mL (3.3 pM) capsid proteins was achieved with convenient UV absorban

214 s are the most asymmetrically arranged major capsid proteins yet observed in virus structures.

215 ase to cleave the P1 polyprotein into mature capsid proteins, but the FMDV 3C protease is toxic to ho

216 motifs in the parechovirus genome that bind capsid proteins, providing approximately 60 specific int

217 Compared with the capsid proteins, the oncogenes E7 and E6 had increased s

218 contacts mediated by N-terminal arms of VP2 capsid proteins, which result in the expansion of the ca

219 in regulating lytic replication, but lacked capsid proteins.

220 et of changes on the interior surface of the capsid reduced viral infectivity by >100-fold and includ

221 ased sequencing of the viral protein 1 (VP1) capsid region is currently the standard method for PV su

222 of the capsid, but little is known about the capsid regions involved in the process.

223 yet important steps that occur between viral capsid release into the cytoplasm and the expression of

224 r intermediates with sizes closer to a T = 4 capsid remained constant.

225 ectrometry of the protein composition of the capsids, revealed that both pUL17 and pUL25 are required

226 equence to successfully package into a viral capsid reveals a complex fitness landscape where the maj

227 (in A-particle) or absence (in procapsid) of capsid-RNA interactions, the two CVA6 particles have ess

228 d here provides a more complete picture of a capsid's structure and therefore can help contribute to

229 The capsid sequence changes in GII.17 strains result in loss

230 Comparison of capsid sequences indicates that GII.17 is evolving at pr

231 of some secondary-structure elements in the capsid shell from which spikes protrude, and a decreased

232 m of a single-stranded RNA enclosed inside a capsid shell, must be reverse transcribed into double-st

233 ing up to 6 nm from the outer surface of the capsid shell.

234 UL17 and pUL25 are required to stabilize the capsid shells at the vertices.

235 ite modified carbon electrode with the viral capsid-specific aptamer.

236 (pDCs) are required for the crosspriming of capsid-specific CD8(+) T cells, whereas other antigen-pr

237 g specific molecules to prevent induction of capsid-specific CD8(+) T cells.

238 Importantly, we find that HAstV-2 capsid spike containing a serine in this loop is immunog

239 e 1.35-A-resolution crystal structure of the capsid spike from one of these HAstV-2 strains.

240 We provide evidence that the HAstV capsid spike is a receptor-binding domain and that the a

241 aternary epitope on each side of the dimeric capsid spike.

242 e long intertwined loops that form the large capsid spikes.

243 speculate on the roles of these residues in capsid stability and postulate that such stabilized VLPs

244 internal stress exceeds the strength of the capsid structure and the capsid breaks open.

245 sence-plays an essential role in determining capsid structure during the self-assembly of CCMV-like p

246 processes must involve small changes in the capsid structure that allow the endocytosed virus to esc

247 With reference to the capsid structure, we speculate on the roles of these res

248 particles have essentially identical atomic capsid structures resembling the uncoating intermediates

249 al polyhedral framework for describing virus capsid structures that is able to account for many of th

250 The size and shape of the capsids studied here are based on the 30-nanometer-diame

251 Moreover, we identify a single capsid substitution that has been adopted by all pandemi

252 nition sites of AAV Nab located on different capsid subunits from one virion.

253 y undescribed molecular interactions between capsid subunits that are crucial to maintain stability i

254 We have identified mutations on the capsid surface and in internal networks that are respons

255 coiled filamentous structure underneath the capsid surface.

256 rmed by the NTD decorate the majority of the capsid surface.

257 VP1 amino acid mutations were located at the capsid surface.

258 of protective antibody epitopes on the HAstV capsid surface.

259 s with many spherical plant viruses, the CNV capsid swells when exposed to alkaline pH and EDTA.

260 Improved capsid tags will facilitate fluorescence microscopy stud

261 Prior studies have identified HIV-1 capsid-targeting compounds that display different mechan

262 chanism of action of the previously reported capsid-targeting HIV-1 inhibitor, Boehringer-Ingelheim c

263 rine antigenic epitopes on an AAV serotype 1 capsid template can evade NAbs without compromising tite

264 mediated by the capsid protein pUL25 and the capsid-tethered tegument protein pUL36.

265 fewer DNA-containing capsids and more empty capsids than wild-type virus.

266 Finally, we identified the region of the SVV capsid that is responsible for receptor recognition usin

267 ion within the mammalian orthoreovirus outer capsid that regulates particle stability.

268 In addition, the unenveloped capsids that accumulated were frequently bound to cytopl

269 the helper's assembly pathway to form small capsids that can only accommodate the smaller SaPI1 geno

270 Here, we describe AAV-PHP.eB and AAV-PHP.S, capsids that efficiently transduce the central and perip

271 s, whereas the putative A-particle and empty capsids that had released the genome had a closed tube-l

272 unds uniquely induced the formation of empty capsids that migrated more slowly in native agarose gel

273 BAs), the BAs promote the formation of empty capsids through specific interaction with HBV core prote

274 lude that pb10 may function to reinforce the capsid thus favouring phage survival in harsh environmen

275 ses, including the ability of the astrovirus capsid to act as an enterotoxin, disrupting the gut epit

276 Binding of the capsid to the nuclear pore complex (NPC) is mediated by

277 fitness cost of amino acid substitutions in capsids to HIV-1 infectivity.

278 its effect on the transport of progeny viral capsids to the nuclear envelope.

279 Transport of incoming viral capsids to the nuclear periphery was unaffected by the c

280 ing the initial entry and transport of viral capsids to the nucleus.

281 eine sequence into the AAV serotype 9 (AAV9) capsid, to permit labelling of viral particles with eith

282 kely increased the chemical stability of the capsid toward ClO2.

283 oration of the roles of tegument proteins in capsid trafficking requires detailed navigational charts

284 albeit poorly defined role in intracellular capsid trafficking.

285 , while a Cys273Ser change appeared to alter capsid transport from the nucleus.

286 serve that BICD2 can bind in vitro-assembled capsid tubes through its CC3 domain.

287 icipate in and regulate HBV virion assembly, capsid uncoating, and covalently closed circular DNA (cc

288 , inhibiting cleavage; TerL release from the capsid upon completion of packaging unlocks the nuclease

289 at herpesvirus small capsid proteins bind to capsids via their amino terminus, whereas the carboxy te

290 neutralizing antibody response to the vector capsid was observed inconsistently.

291 s was greatly diminished, and DNA-containing capsids were not observed.

292 Also, increased proportions of empty capsids were observed in the cytoplasm, suggesting a rol

293 ormation for the procapsid and genome-filled capsids, whereas the putative A-particle and empty capsi

294 ular and enteric pathogens that form complex capsids, which are assembled from seven different struct

295 The bocaparvovirus capsids, which display different tissue tropisms, have f

296 o that is optimal for packaging the RNA into capsids, while also containing capacity for coding for a

297 psid protein (CA) assembles into polymorphic capsids, whose architecture, assembly, and stability are

298 imulations reveal critical details about the capsid with implications to biological function.

299 ssRNA genome of 3,569 bases is enclosed in a capsid with one maturation protein monomer and 89 coat p

300 he CTD in empty HBV virions (i.e., enveloped capsids with no RNA or DNA) was found to be phosphorylat