ライフサイエンスコーパス: carbamoyl phosphate

1 l reactions but not the overall synthesis of carbamoyl phosphate.

2 biosynthesis by catalyzing the production of carbamoyl phosphate.

3 orylation of carbamate to the final product, carbamoyl phosphate.

4 droxybenzoic acid (AHBA), D-glucosamine, and carbamoyl phosphate.

5 ichaelis constants for either bicarbonate or carbamoyl phosphate.

6 erized with or without prior incubation with carbamoyl phosphate.

7 nucleotides used in the overall synthesis of carbamoyl phosphate.

8 rst ATP utilized in the overall synthesis of carbamoyl phosphate.

9 e intermediate during the final formation of carbamoyl phosphate.

10 re diminished in their ability to synthesize carbamoyl phosphate.

11 mino group of Asp and the carbonyl carbon of carbamoyl phosphate.

12 rmational change induced upon the binding of carbamoyl phosphate.

13 le to utilize glutamine for the synthesis of carbamoyl phosphate.

14 ammonia for the ATP-dependent generation of carbamoyl phosphate.

15 cluding acetyl phosphate, benzoyl phosphate, carbamoyl phosphate, 2-methoxybenzoyl phosphate, and pho

16 A saturating concentration of carbamoyl phosphate alone has little influence on the sm

17 nit contains an N-terminal domain that binds carbamoyl phosphate and a C-terminal domain that binds L

18 vity and decreased substrate affinity toward carbamoyl phosphate and aspartate compared to the corres

19 ropic cooperativity, and the binding of both carbamoyl phosphate and aspartate were extremely comprom

20 little change in the Km for the substrates, carbamoyl phosphate and aspartate.

21 n mechanism is committed to the formation of carbamoyl phosphate and is not subject to hydrolysis.

22 ing established that the natural substrates, carbamoyl phosphate and L-aspartate, do not induce in th

23 identify the enzymatic synthesis of N-amino carbamoyl phosphate and N-hydroxy carbamoyl phosphate fr

24 lts in impaired synthesis of citrulline from carbamoyl phosphate and ornithine.

25 t catalyzes the synthesis of citrulline from carbamoyl phosphate and ornithine.

26 of the mutants were unable to synthesize any carbamoyl phosphate and the rest were severely crippled.

27 of ligands, in the presence of the substrate carbamoyl phosphate, and in the presence of the bisubstr

28 xaloacetate, the phosphorylation of MgADP by carbamoyl phosphate, and the bicarbonate-dependent ATPas

29 absence and presence of the first substrate carbamoyl phosphate are reported.

30 less negatively charged than its precursors, carbamoyl phosphate, aspartate, or carbamoyl aspartate.

31 in proteins which were unable to synthesize carbamoyl phosphate at a significant rate.

32 There is no preferential partitioning of carbamoyl phosphate between the arginine and pyrimidine

33 polypeptide chain folds into two domains, a carbamoyl phosphate binding domain and an L-ornithine bi

34 lex with citric acid bound in the postulated carbamoyl phosphate binding site, was determined in two

35 ghly conserved residue that is essential for carbamoyl-phosphate binding.

36 y two long interdomain helices: the putative carbamoyl phosphate-binding domain and a binding domain

37 The putative carbamoyl phosphate-binding site is similar to those in

38 l change that interferes with the binding of carbamoyl phosphate but has little effect once carbamoyl

39 bsaturating amounts of PALA or succinate and carbamoyl phosphate) caused a hyperbolic increase and de

40 rococcus abyssi demonstrate the existence of carbamoyl phosphate channeling in both the pyrimidine an

41 following the ATP synthesis reaction at low carbamoyl phosphate concentration.

42 topped-flow experiments, using aspartate and carbamoyl phosphate, confirm that the change in excimer

43 ucleotide biosynthesis, the reaction between carbamoyl phosphate (CP) and l-aspartate to form N-carba

44 responds to the position of the phosphate of carbamoyl phosphate (CP) and the position of the phospho

45 Carbamoyl phosphate (CP) has a half-life for thermal dec

46 st, the bicarbonate-dependent ATPase and the carbamoyl phosphate-dependent ATP synthetase activities

47 The carboxy phosphate (residues 1-400) and carbamoyl phosphate domains (residues 553-933) also cont

48 e switch mechanism includes the synthesis of carbamoyl phosphate entirely within a single nucleotide

49 mation of MgATP and carbamate from MgADP and carbamoyl phosphate, Escherichia coli carbamoyl phosphat

50 e the absolute requirement of the binding of carbamoyl phosphate for the creation of the high-affinit

51 oyl-phosphate synthetase 1 activity produces carbamoyl phosphate for urea synthesis, and deficiency r

52 t catalyzes glutamine-dependent formation of carbamoyl phosphate for urea synthesis.

53 The absence of an initial burst of carbamoyl phosphate formation eliminates product release

54 of the partial reactions, the diminution of carbamoyl phosphate formation, and the percentage of the

55 Escherichia coli catalyzes the formation of carbamoyl phosphate from 2 mol of ATP, bicarbonate, and

56 a 120-kDa synthetase domain (CPS) that makes carbamoyl phosphate from ATP, bicarbonate, and ammonia u

57 of CPS.A and CPS.B proteins that synthesized carbamoyl phosphate from ATP, bicarbonate, and ammonia.

58 Escherichia coli catalyzes the formation of carbamoyl phosphate from bicarbonate, glutamine, and two

59 Escherichia coli catalyzes the formation of carbamoyl phosphate from bicarbonate, glutamine, and two

60 synthetase (CPS) catalyzes the formation of carbamoyl phosphate from bicarbonate, glutamine, and two

61 phate synthetase catalyzes the production of carbamoyl phosphate from bicarbonate, glutamine, and two

62 synthetase (CPS) catalyzes the formation of carbamoyl phosphate from glutamine, bicarbonate, and 2 m

63 of N-amino carbamoyl phosphate and N-hydroxy carbamoyl phosphate from hydroxylamine and hydrazine.

64 y 118 kDa) and catalyzes the biosynthesis of carbamoyl phosphate from MgATP, bicarbonate, and glutami

65 channeling ensures the efficient transfer of carbamoyl phosphate from the active site of CPSase to th

66 Escherichia coli catalyzes the production of carbamoyl phosphate from two molecules of Mg2+ATP, one m

67 Escherichia coli catalyzes the formation of carbamoyl phosphate from two molecules of MgATP, bicarbo

68 gh rapidly degraded at high temperature, the carbamoyl phosphate generated in situ by A. aeolicus car

69 nithine which attacks the carbonyl carbon of carbamoyl phosphate in the enzyme-catalyzed reaction.

70 hosphate synthetase-1 (CPS1), which produces carbamoyl phosphate in the mitochondria from ammonia and

71 d-type enzyme is required for the binding of carbamoyl phosphate in the proper orientation so as to i

72 led reaction, the effective concentration of carbamoyl phosphate in the vicinity of the ATCase active

73 must perform during the overall synthesis of carbamoyl phosphate in the wild type enzyme and the spec

74 icarbonate during the enzymatic synthesis of carbamoyl phosphate, indicating that any carbon-containi

75 ontrast to the wild-type enzyme, addition of carbamoyl phosphate induced a significant alteration in

76 nally, the data indicate that the binding of carbamoyl phosphate induces conformational changes that

77 rbamoyl phosphate but has little effect once carbamoyl phosphate is bound.

78 miting step for the steady-state assembly of carbamoyl phosphate is either the formation, migration,

79 uples the two partial reactions such that no carbamoyl phosphate is produced.

80 the catalytic cysteine residue, can generate carbamoyl phosphate only in the presence of free ammonia

81 ng the altered CAD with the ATCase substrate carbamoyl phosphate or the bisubstrate analogue N-phosph

82 action catalyzed by biotin carboxylase where carbamoyl phosphate reacts with ADP was decreased 100-fo

83 the catalytic activity for the synthesis of carbamoyl phosphate relative to the wild type CPS, respe

84 dual partial reactions, overall synthesis of carbamoyl phosphate required a homodimer of CPS.A or CPS

85 nfirmed the hypothesis that the synthesis of carbamoyl phosphate requires the concerted action of the

86 Vaccination with the carbamoyl phosphate synthase (CPS) mutant strain of Toxo

87 ioxin resulted in concomitant recruitment of carbamoyl phosphate synthase 1 (CPS1) to the NC-XRE.

88 ed to increase the level of succinylation on carbamoyl phosphate synthase 1, which is a known target

89 erence-mediated knockdown of a mitochondrial carbamoyl phosphate synthase impairs the response of nit

90 acetylglutamate, the obligatory activator of carbamoyl phosphate synthase-1 (CPS1).

91 ammonemia from reduced expression of hepatic carbamoyl phosphate synthase-I.

92 arbamoylases, and an internal duplication in carbamoyl phosphate synthase.

93 minases is demonstrated for Escherichia coli carbamoyl phosphate synthase.

94 he lead variant on 2q24 (rs715) localizes to carbamoyl-phosphate synthase 1 (CPS1), which encodes a m

95 y facilitating pyrimidine synthesis via CAD (carbamoyl-phosphate synthase 2, aspartate transcarbamyla

96 xpression profile of the c-Myc target genes, carbamoyl-phosphate synthase-aspartate carbamoyltransfer

97 bited complex I of the respiratory chain and carbamoyl-phosphate synthase-I (CPS-I), with an EC(50) a

98 Carbamoyl-phosphate synthase/aspartate carbamoyltransfer

99 The partial recovery of carbamoyl phosphate synthesis activity in the double mut

100 in binding the two ATP molecules needed for carbamoyl phosphate synthesis and a carboxyl-terminal do

101 alues of K(m) for glutamine, but the overall carbamoyl phosphate synthesis reaction is unperturbed.

102 Carbamoyl phosphate synthesis requires the concerted act

103 tation, the rate of glutamine hydrolysis and carbamoyl phosphate synthesis were no longer coordinated

104 d HCO(3)(-), the other substrates needed for carbamoyl phosphate synthesis, bind to the synthetase do

105 5L exhibited a substantially reduced rate of carbamoyl phosphate synthesis, but the rate of ATP turno

106 Ser(44) --> Ala mutant than that needed for carbamoyl phosphate synthesis.

107 circuit that phases glutamine hydrolysis and carbamoyl phosphate synthesis.

108 he separately cloned subdomains can catalyze carbamoyl phosphate synthesis.

109 ifferent ATP-dependent reactions involved in carbamoyl phosphate synthesis.

110 valent and could each independently catalyze carbamoyl phosphate synthesis.

111 ATP-dependent partial reactions involved in carbamoyl phosphate synthesis.

112 hree reactions involved in ammonia-dependent carbamoyl phosphate synthesis.

113 ne or free ammonia as the nitrogen donor for carbamoyl phosphate synthesis.

114 ation of ATP bound to domain C is coupled to carbamoyl-phosphate synthesis at domain B via a nucleoti

115 imiting step in this pathway is catalysed by carbamoyl phosphate synthetase (CPS II), part of the mul

116 ein (MAP-2), myosin light chain (MYL-1), and carbamoyl phosphate synthetase (CPS III).

117 DP and carbamoyl phosphate, Escherichia coli carbamoyl phosphate synthetase (CPS) binds MgADP with a

118 Carbamoyl phosphate synthetase (CPS) catalyzes the forma

119 Carbamoyl phosphate synthetase (CPS) catalyzes the forma

120 Carbamoyl phosphate synthetase (CPS) catalyzes the produ

121 analysis of the allosteric responsiveness of carbamoyl phosphate synthetase (CPS) from E. coli was pe

122 Carbamoyl phosphate synthetase (CPS) from Escherichia co

123 Carbamoyl phosphate synthetase (CPS) from Escherichia co

124 The heterodimeric carbamoyl phosphate synthetase (CPS) from Escherichia co

125 Carbamoyl phosphate synthetase (CPS) from Escherichia co

126 Carbamoyl phosphate synthetase (CPS) from Escherichia co

127 rt of carbamate through the large subunit of carbamoyl phosphate synthetase (CPS) from Escherichia co

128 Carbamoyl phosphate synthetase (CPS) from Escherichia co

129 Carbamoyl phosphate synthetase (CPS) from Escherichia co

130 The X-ray crystal structure of carbamoyl phosphate synthetase (CPS) from Escherichia co

131 Carbamoyl phosphate synthetase (CPS) is a member of the

132 Carbamoyl phosphate synthetase (CPS) plays a key role in

133 The transfer of ammonia in carbamoyl phosphate synthetase (CPS) was investigated by

134 st three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcar

135 individual mutant lines deficient in either carbamoyl phosphate synthetase (CPS), the first enzyme i

136 ayed by this class of enzymes is provided by carbamoyl phosphate synthetase (CPS).

137 l phosphate generated in situ by A. aeolicus carbamoyl phosphate synthetase (CPSase) was channeled to

138 a large multifunctional protein that carries carbamoyl phosphate synthetase (CPSase), aspartate trans

139 Human carbamoyl phosphate synthetase (hCPS) has evolved critic

140 Carbamoyl phosphate synthetase 1 (CPS1) is a liver-speci

141 the mitochondrial matrix and interacts with carbamoyl phosphate synthetase 1 (CPS1), an enzyme, cata

142 Systematic optimization of a carbamoyl phosphate synthetase 1 derived, glutarylated p

143 ylates and activates a mitochondrial enzyme, carbamoyl phosphate synthetase 1, which mediates the fir

144 proteins 70 and 90 (HSP-70; HSP-90), and the carbamoyl phosphate synthetase 2/aspartate transcarbamyl

145 he inhibitor UTP and the activator PRPP, the carbamoyl phosphate synthetase activity is controlled by

146 the sequential action of MAPK and PKA on the carbamoyl phosphate synthetase activity of CAD.

147 Carbamoyl phosphate synthetase catalyzes the hydrolysis

148 Carbamoyl phosphate synthetase catalyzes the production

149 The carbamoyl phosphate synthetase domain of the multifuncti

150 Carbamoyl phosphate synthetase from E. coli catalyzes th

151 ribe the X-ray crystallographic structure of carbamoyl phosphate synthetase from E. coli in which His

152 Carbamoyl phosphate synthetase from Escherichia coli cat

153 Carbamoyl phosphate synthetase from Escherichia coli cat

154 x-ray crystal structure of the heterodimeric carbamoyl phosphate synthetase from Escherichia coli has

155 Carbamoyl phosphate synthetase I (CPSase I; EC 6.3.4.16)

156 arginine serum levels on chromosome 2 at the carbamoyl phosphate synthetase I locus, on chromosome 5

157 ine nucleotide synthesis is catalyzed by the carbamoyl phosphate synthetase II (CPSase) domain of CAD

158 Carbamoyl phosphate synthetase II (CPSII) is part of car

159 the virulence of T. gondii mutants that lack carbamoyl phosphate synthetase II (uracil auxotrophs) to

160 Carbamoyl phosphate synthetase is isolated from Escheric

161 mately 25 A in length, whereas the tunnel in carbamoyl phosphate synthetase is nearly 100 A long.

162 The arginine-specific carbamoyl phosphate synthetase of Saccharomyces cerevisi

163 , cytidine 5'-triphosphate (CTP) synthetase, carbamoyl phosphate synthetase, and phosphoribosyl pyrop

164 mensional structures of tryptophan synthase, carbamoyl phosphate synthetase, glutamine phosphoribosyl

165 n some cases, such as biotin carboxylase and carbamoyl phosphate synthetase, the B-domains move signi

166 KL cells express the urea cycle enzyme carbamoyl phosphate synthetase-1 (CPS1), which produces

167 is similar to the folding of this domain in carbamoyl phosphate synthetase.

168 ransferase type I domain of Escherichia coli carbamoyl phosphate synthetase.

169 r the tunneling of ammonia within the native carbamoyl phosphate synthetase.

170 specifying the small subunit of Arg-specific carbamoyl phosphate synthetase.

171 codes the small subunit of arginine-specific carbamoyl phosphate synthetase.

172 the G359F (small subunit) mutant protein of carbamoyl phosphate synthetase.

173 ned a 240 kDa protein that was identified as carbamoyl phosphate synthetase/aspartate transcarbamoyla

174 l phosphate synthetase II (CPSII) is part of carbamoyl phosphate synthetase/aspartate transcarbamoyla

175 Carbamoyl phosphate synthetase/aspartate transcarbamylas

176 Carbamoyl-phosphate synthetase (CPS) from Escherichia co

177 he kinetics of the coupled reactions between carbamoyl-phosphate synthetase (CPSase) and both asparta

178 Carbamoyl-phosphate synthetase (CPSase) consists of a 12

179 Escherichia coli carbamoyl-phosphate synthetase (CPSase) is comprised of

180 hyperthermophile, has neither a full-length carbamoyl-phosphate synthetase (CPSase) resembling the e

181 In mitochondria, carbamoyl-phosphate synthetase 1 activity produces carba

182 The tri-functional enzyme contains carbamoyl-phosphate synthetase 2 (CPS2), aspartate trans

183 which directly phosphorylates S1859 on CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamo

184 Moreover, EGFR signaling activated carbamoyl-phosphate synthetase 2, aspartate transcarbamy

185 hatidylinositol 4-kinases (PI4KA and PI4KB), carbamoyl-phosphate synthetase 2, aspartate transcarbamy

186 Cytoplasmic carbamoyl-phosphate synthetase 2, however, is part of a

187 uted to altered allosteric regulation of the carbamoyl-phosphate synthetase activity of CAD (carbamoy

188 Carbamoyl-phosphate synthetase catalyzes the production

189 Carbamoyl-phosphate synthetase consists of an amidotrans

190 oncerted action of the glutaminase (GLN) and carbamoyl-phosphate synthetase domains of CAD.

191 The frequencies of the carbamoyl-phosphate synthetase genotypes in the study po

192 Carbamoyl-phosphate synthetase I (CPSase I) catalyzes th

193 ferential plasma proteins detected by iTRAQ, carbamoyl-phosphate synthetase I (CPSI, related to urea

194 d enzymes ornithine carbamoyltransferase and carbamoyl-phosphate synthetase III (CPSase III) are indu

195 Carbamoyl-phosphate synthetase III (CPSase III) of Squal

196 the first enzyme in the urea cycle pathway, carbamoyl-phosphate synthetase III (CPSase III), is too

197 Mammalian carbamoyl-phosphate synthetase is part of carbamoyl-phos

198 this closed form of biotin carboxylase with carbamoyl-phosphate synthetase is presented.

199 Ser(44) GLN domain and the Escherichia coli carbamoyl-phosphate synthetase large subunit had little

200 skewed distribution of the genotypes for the carbamoyl-phosphate synthetase variants at position 1405

201 trations of amino acids and genotypes of the carbamoyl-phosphate synthetase variants were determined

202 e thioester intermediate of Escherichia coli carbamoyl-phosphate synthetase, indicates that the subst

203 for threonine at position 1405 [T1405N]) in carbamoyl-phosphate synthetase, which controls the rate-

204 urea cycle--in particular, the efficiency of carbamoyl-phosphate synthetase--may contribute to the av

205 an carbamoyl-phosphate synthetase is part of carbamoyl-phosphate synthetase-aspartate carbamoyltransf

206 bamoyl-phosphate synthetase activity of CAD (carbamoyl-phosphate synthetase-aspartate carbamoyltransf

207 d c-myc, dihydrofolate reductase (DHFR), and carbamoyl-phosphate synthetase-aspartate transcarbamoyl-

208 ession of mature hepatocytic markers such as carbamoyl-phosphate synthetase1 and several cytochrome P

209 oning was used to identify a mutation in the carbamoyl-phosphate synthetase2-aspartate transcarbamyla

210 Depending on their physiological role, carbamoyl phosphate synthetases (CPSs) use either glutam

211 Carbamoyl phosphate synthetases (CPSs) utilize either gl

212 Bacillus stearothermophilus contains two carbamoyl-phosphate synthetases (CPS), one specific for

213 Although carbamoyl-phosphate synthetases (CPSs) share sequence id

214 Carbamoyl-phosphate synthetases (CPSs) utilize two molec

215 The B-domain of the carbamoyl phosphate synthetic component of the large sub

216 lves referred to as the carboxyphosphate and carbamoyl phosphate synthetic components.

217 phate synthetase catalyzes the production of carbamoyl phosphate through a reaction mechanism requiri

218 tase from E. coli catalyzes the synthesis of carbamoyl phosphate through a series of four reactions o

219 ast step in this pathway, converting ADP and carbamoyl phosphate to ATP and ammonium carbamate.

220 inine dihydrolase pathway converting ADP and carbamoyl phosphate to ATP and carbamate.

221 The inability of carbamoyl phosphate to create the high-affinity binding

222 gue succinate, in the presence of saturating carbamoyl phosphate, to the pyrenelabeled enzyme caused

223 our other metabolites, S-adenosylmethionine, carbamoyl phosphate, UDP-glucose, and Delta(2)-isopenten

224 l-molecule phosphodonors acetyl phosphate or carbamoyl phosphate under conditions in which a control

225 he larger subunit catalyzes the formation of carbamoyl phosphate using 2 mol of ATP, bicarbonate, and

226 This mutant is unable to synthesize carbamoyl phosphate using glutamine as a nitrogen source

227 However, carbamoyl phosphate was able to shift the structure of t

228 teady-state time course for the formation of carbamoyl phosphate was linear with an overall rate cons

229 However, neither ammonia nor carbamoyl phosphate was produced, which implies that pur

230 formation of phosphate, ADP, glutamate, and carbamoyl phosphate were determined.

231 synthetase (CPS) catalyzes the production of carbamoyl phosphate which is subsequently employed in th

232 Escherichia coli catalyzes the formation of carbamoyl phosphate, which is subsequently employed in b