ライフサイエンスコーパス: carbinolamine

1 er in another step, likely hydrolysis of the carbinolamine.

2 arbinolamine nitrogen to give the protonated carbinolamine.

3 ble of existing in equilibrium with a cyclic carbinolamine.

4 -1, respectively, in dehydrating the neutral carbinolamine.

5 roups and give rise to 5- or 6-membered ring carbinolamines.

6 m constant K1 = 1.87 x 10(3) M(-1)) with the carbinolamine 2-chloro-1-(chloroamino)ethanol.

7 such that the oxygen atoms analogous to the carbinolamine and beta-hydroxyl oxygens are positioned n

8 ambiguously identify the postulated covalent carbinolamine and Schiff base intermediates in the aldol

9 species result in the formation of both the carbinolamine and the hydrazone derivatives.

10 ial formation of alpha-iminoglutarate, alpha-carbinolamine, and alpha-ketoglutarate-reduced coenzyme

11 ls of solvation that would otherwise inhibit carbinolamine, and thus imine, formation.

12 e derived aldehydes to form 5- or 6-membered carbinolamines are critical determinants of biologic pot

13 We have identified carbinolamines as a new class of alternate substrate.

14 ve stability of the interconvertible N10-C11 carbinolamine, carbinolamine methyl ether, and imine for

15 ) and the ultrared as the enzyme-NADPH-alpha-carbinolamine complex (ERC).

16 -alpha-iminoglutarate and enzyme-NADPH-alpha-carbinolamine complexes at concentrations whose sum acco

17 ent forms of the known alpha-imino and alpha-carbinolamine complexes in which the active site cleft i

18 alpha-iminoglutarate and highly red-shifted carbinolamine complexes observed in both reactions, the

19 In contrast, dehydration of the carbinolamine cross-link to an imine (Schiff base) cross

20 amino group established unambiguously that a carbinolamine cross-link was not formed.

21 These sequence-specific carbinolamine cross-links are anticipated to interfere w

22 otein with distant homology to pterin-4alpha-carbinolamine dehydratase (PCD) enzymes.

23 Pterin-4a-carbinolamine dehydratase (PCD) is a highly conserved en

24 s encoding phenylalanine hydroxylase (PhhA), carbinolamine dehydratase (PhhB), and aromatic aminotran

25 ion has been found, supporting the idea that carbinolamine dehydratase activity is not required for H

26 oH2 forms a tetramer, displays pterin-4alpha-carbinolamine dehydratase activity, and binds HNF1alpha

27 Pterin 4a-carbinolamine dehydratase is bifunctional in mammals.

28 osa PhhA plus the recycling enzyme pterin 4a-carbinolamine dehydratase, PhhB, rescues tyrosine auxotr

29 ting assays further indicated that pterin-4a-carbinolamine dehydratase, which regenerates the AAH cof

30 atalase, glutathione peroxidase, and 4 alpha-carbinolamine dehydratase.

31 tivity of the bifunctional protein pterin-4a-carbinolamine dehydratase/dimerization cofactor for hepa

32 ed a novel deletion in PCBD1 (pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepat

33 tocyte nuclear factor 1 (DCoH)/pterin-4alpha-carbinolamine dehydratases (PCD)-like protein is the cau

34 Pterin-4a-carbinolamine dehydratases (PCDs) recycle oxidized pteri

35 The carbinolamine dehydration step was found to be acid/base

36 amino group may also enhance the rate of the carbinolamine dehydration step.

37 ition reaction resulting in formation of the carbinolamine derivative.

38 um 40% of the DNA was cross-linked, with the carbinolamine form of the cross-link predominating.

39 ase, which allowed the rates of both initial carbinolamine formation (as part of the imination step)

40 ion by acting as a proton shuttle during the carbinolamine formation step, which enables diamines to

41 MTOX oxidation of the carbinolamine formed with L-tryptophan and formaldehyde

42 ith N-methyl-L-tryptophan, sarcosine, or the carbinolamine formed with L-tryptophan and formaldehyde.

43 s tentatively assigned to protonation of the carbinolamine guanidinium system.

44 y the same group accepting a proton from the carbinolamine hydroxyl to generate alpha-Kg and lysine.

45 in protonation of the hydroxyl group of the carbinolamine in the dehydration step, catalyzing Schiff

46 evidence is presented for the formation of a carbinolamine interchain cross-link in 5'-CpG-3' sequenc

47 esidue or position in the protonation of the carbinolamine intermediate and dehydration of the Schiff

48 strongly for the N-dealkylation of 2a via a carbinolamine intermediate formed by a conventional C-hy

49 to facilitate formation and breakdown of the carbinolamine intermediate to give the Schiff base and t

50 -hexanoic acid, which seems to form a stable carbinolamine intermediate with Lys263.

51 catalyzed step, most likely protonation of a carbinolamine intermediate, is also significantly rate c

52 ction proceeds with C-N bond cleavage in the carbinolamine intermediate, shows excellent functional g

53 hiff base carbon of saccharopine to form the carbinolamine intermediate.

54 acilitate proton transfer from Lys-53 to the carbinolamine intermediate.

55 ) at C-H bonds in 1-4 leads to nonrearranged carbinolamine intermediates and thereby to "ordinary" N-

56 part, explain the thermal stability of this carbinolamine interstrand cross-link and the stereochemi

57 ally stable surrogates for the corresponding carbinolamine interstrand cross-links arising from the c

58 Collapse of the carbinolamine is then facilitated by the same group acce

59 Molecular modeling suggested that the carbinolamine linkage should be capable of maintaining W

60 is the dG linked to the alpha-carbon of the carbinolamine linkage, and Y(19) is the dG linked to the

61 is the dG linked to the gamma-carbon of the carbinolamine linkage; the cross-link is in the 5'-CpG-3

62 the interconvertible N10-C11 carbinolamine, carbinolamine methyl ether, and imine forms of PBDs.

63 p is likely protonation/deprotonation of the carbinolamine nitrogen formed as an intermediate in imin

64 s a general acid and donates a proton to the carbinolamine nitrogen to give the protonated carbinolam

65 isotope effects reveal that collapse of the carbinolamine (or gem-diamine) to give the final product

66 ct of chain length, oxygen substitution, and carbinolamine ring size on analogue potency.

67 m (3aS)-N8-benzylnoresermethole (-)12 by the carbinolamine route.

68 hat the cross-link is a mixture of imine and carbinolamine structures.

69 hat it can act as a nucleophile in forming a carbinolamine upon attack of the carbonyl of AASA.

70 ve of 1b, which cannot give rise to a cyclic carbinolamine, was 2 orders of magnitude less potent tha

71 ues capable of forming 7-, 8-, or 9-membered carbinolamines were markedly less active.