ライフサイエンスコーパス: carboxysome

1 h which metabolites presumably pass into the carboxysome.

2 to be structurally characterized from a beta-carboxysome.

3 l beta-cyanobacterial genomes is part of the carboxysome.

4 egularly spaced subcellular compartment, the carboxysome.

5 isCO), the major enzyme component inside the carboxysome.

6 ernatively, EutG may recycle NADH within the carboxysome.

7 in other organisms) as shell proteins of the carboxysome.

8 odel of the internal arrangement of the beta-carboxysome.

9 Small granular bodies were also seen within carboxysomes.

10 nsity frequently connects granules to nearby carboxysomes.

11 stered into polyhedral protein bodies called carboxysomes.

12 e compared with those of intact and ruptured carboxysomes.

13 f the enzyme into polyhedral organelles, the carboxysomes.

14 rotein shell that is related to the shell of carboxysomes.

15 The mutant does not possess carboxysomes.

16 istinct from CsoS2A in the assembly of alpha-carboxysomes.

17 nside proteinaceous microcompartments called carboxysomes.

18 nobacteria with Form-IB Rubisco contain beta-carboxysomes.

19 tep in the biogenesis of cyanobacterial beta-carboxysomes.

20 sequence similarity to the shell proteins of carboxysomes (a polyhedral organelle involved in autotro

21 a process occurs during the formation of the carboxysome, a bacterial microcompartment that assembles

22 The best-characterized BMC is the carboxysome, a central part of the carbon-concentrating

23 We have constructed a synthetic mimic of the carboxysome, a cyanobacterial carbon-fixing organelle.

24 ototypical bacterial microcompartment is the carboxysome, a protein shell for sequestering carbon fix

25 s encode homologues of shell proteins of the carboxysome, an organelle shown (in other organisms) to

26 This process requires the carboxysome, an organelle-like proteinaceous microcompar

27 We show that CcmN localizes to the carboxysome and is essential for carboxysome biogenesis.

28 th its apparent genomic association with the carboxysome and its constituents.

29 alizations of the outer protein shell of the carboxysome and its internal cargo.

30 m for BMC-associated metabolism, joining the carboxysome and metabolosome.

31 The carboxysome and other bacterial microcompartments (BMCs)

32 tulated pore-forming protein CsoS1D with the carboxysome and show how it may modulate function.

33 Given that carboxysomes and architecturally related bacterial organ

34 ary and structural differences between alpha-carboxysomes and beta-carboxysomes, we found that the tw

35 Fundamental to engineering carboxysomes and other BMCs for applications in plant sy

36 Within the scope of synthetic biology, carboxysomes and other BMCs hold even greater potential

37 ns suggest a functional relationship between carboxysomes and polyphosphate granules.

38 r components, including thylakoid membranes, carboxysomes and polyribosomes, as well as phages, insid

39 eleton to control the spatial arrangement of carboxysomes and to optimize the metabolic process of ca

40 obacteria with Form-IA Rubisco contain alpha-carboxysomes, and cyanobacteria with Form-IB Rubisco con

41 that much of the enzyme is sequestered into carboxysomes, and that the genes for the enzyme, cbbL an

42 formation of polyhedral organelles known as carboxysomes, and two encode proteins that appear distan

43 observed that the shell proteins of the beta-carboxysome are able to assemble in plant chloroplasts i

44 wed that some of the shell components of the carboxysome are produced, which may explain the presence

45 Carboxysomes are bacterial microcompartments that enhanc

46 Carboxysomes are bounded by a proteinaceous outer shell

47 Typically, alpha-carboxysomes are genetically encoded as a single operon

48 Carboxysomes are organelle-like polyhedral bodies found

49 Carboxysomes are polyhedral bodies consisting of a prote

50 Carboxysomes are polyhedral inclusion bodies that play a

51 Carboxysomes are proteinaceous bacterial microcompartmen

52 led biosynthesis and ordered organization of carboxysomes are vital to the CO2-fixing activity of cya

53 Here, we describe the stages of carboxysome assembly and the requisite gene products nec

54 and decreases the genetic load required for carboxysome assembly in heterologous systems.

55 n these observations, we propose a model for carboxysome assembly in which the shell and the internal

56 Carboxysome assembly involves a series of protein-protei

57 We propose that the principles of carboxysome assembly that we have uncovered extend to di

58 ng of protein-protein interactions governing carboxysome assembly, but also establish a platform to s

59 The alpha-carboxysome-associated ortholog, CsoCbbQ, from Halothiob

60 that this protein, which we refer to as the carboxysome-associated PII protein (CPII), represents a

61 A (or CsoS4A), from the two known classes of carboxysomes, at resolutions of 2.4 and 2.15 angstroms.

62 l RuBisCO to be encapsulated into modern-day carboxysomes, bacterial organelles central to the cyanob

63 indicating a close coordination between beta-carboxysome biogenesis and photosynthetic electron trans

64 izes to the carboxysome and is essential for carboxysome biogenesis.

65 ential knowledge for us to modulate the beta-carboxysome biosynthesis and function in cyanobacteria.

66 However, little is known about how carboxysome biosynthesis and spatial positioning are phy

67 We demonstrated that beta-carboxysome biosynthesis is accelerated in response to i

68 M composed of one or two HCO3(-) pumps and a carboxysome, but its functionality has not been examined

69 We show here that carboxysomes, CO(2)-fixing microcompartments encoded by

70 Mutations were also recovered in ccmK4, a carboxysome coat protein homologue, and me, the decarbox

71 Carboxysomes compartmentalize the enzyme ribulose-1,5-bi

72 Our study indicates that carboxysome composition is probably more complex than wa

73 mic evidence have important implications for carboxysome composition, structure, and function.

74 Carboxysomes consist of a protein shell that encapsulate

75 It is present in all alpha-carboxysome containing bacteria and has homologs in alga

76 Protein domains native to the carboxysome core were used to encapsulate foreign cargo

77 based on protein domain interactions in the carboxysome core.

78 As a metabolic module for carbon fixation, carboxysomes could be transferred to eukaryotic organism

79 vision defects, glycogen limitation, lack of carboxysomes, deteriorated thylakoids and accumulation o

80 sizes and enzyme packings of alpha- and beta-carboxysomes each constitute an optimal compartmentaliza

81 xation in C4 plants or in the cyanobacterial carboxysome, enhances the activity of inefficient enzyme

82 Carboxysomes exhibited greater size, shape, and composit

83 Capsids, carboxysomes, exosomes, vacuoles and other nanoshells ea

84 rotein shell; prominent examples include the carboxysome for CO2 fixation and catabolic microcompartm

85 Well-characterized BMCs include carboxysomes for CO2-fixation, and propanediol- and etha

86 e especially useful for developing synthetic carboxysomes for plant engineering.

87 Deletion of the peptide abolishes carboxysome formation, indicating that its interaction w

88 ppear distantly related to those involved in carboxysome formation.

89 the individual roles of CsoS2B and CsoS2A on carboxysome formation.

90 lly replaces four gene products required for carboxysome formation.

91 to explore the possibility of producing beta-carboxysomes from Synechococcus elongatus PCC7942, a mod

92 the three-dimensional structure of purified carboxysomes from Synechococcus species strain WH8102 as

93 cessful purification and characterization of carboxysomes from the marine cyanobacterium Prochlorococ

94 Some models of carboxysome function depend on specific contacts between

95 nowledge, the first direct comparison of the carboxysome function from alpha-cyanobacteria and beta-c

96 l function of these species and that similar carboxysome function may be possessed by each carboxysom

97 a novel RuBisCO chaperone integral to alpha-carboxysome function.

98 mity alone may not be the key determinant in carboxysome function.

99 hotomixotrophic growth, and the abundance of carboxysome gene (rbcL, ccmK1, ccmL) and ndhF4 transcrip

100 Similar results were observed for carboxysome gene transcription and assembly, as assayed

101 We found that carboxysomes generally cluster into distinct groups with

102 The carboxysome genes are located adjacent to cbb genes, whi

103 In addition to carboxysome genes, two novel genes (Tcr_1019 and Tcr_131

104 the different Rubisco forms present in each carboxysome had almost identical kinetic parameters.

105 Complicating the study of cyanobacterial carboxysomes has been the inability to obtain homogeneou

106 ggesting that the S. enterica organelles and carboxysomes have a related multiprotein shell.

107 teria, bacterial microcompartments, known as carboxysomes, improve the efficiency of photosynthesis b

108 l Rubisco and progress toward synthesizing a carboxysome in chloroplasts.

109 cells undergoing division evenly segregated carboxysomes in a nonrandom process.

110 Expression of carboxysomes in E. coli resulted in the production of ic

111 re sufficient for heterologous expression of carboxysomes in Escherichia coli, opening the door to de

112 We have aligned and averaged carboxysomes in several size classes from the 3D tomogra

113 The widely accepted models for the role of carboxysomes in the carbon-concentrating mechanism of au

114 Likewise, the spatial organization of carboxysomes in the cell correlates with the redox state

115 Using electron cryotomography, we examined carboxysomes in their native states within intact cells

116 We found that while the sizes of individual carboxysomes in this organism varied from 114 nm to 137

117 Finally, we observed carboxysomes in various stages of assembly, as well as f

118 Characterization of purified synthetic carboxysomes indicated that they were well formed in str

119 ic electron flow impairs the accumulation of carboxysomes, indicating a close coordination between be

120 eding diffusion of CO(2) into and out of the carboxysome interior.

121 nts establish the feasibility of introducing carboxysomes into chloroplasts for the potential compart

122 sign and synthetic engineering of functional carboxysomes into higher plants to improve photosynthesi

123 on the viability of transplanting functional carboxysomes into the C3 chloroplast.

124 obacterial CO2 -concentrating mechanism, the carboxysome, into chloroplasts is an approach to enhance

125 The carboxysome is a bacterial microcompartment that enhance

126 The carboxysome is a bacterial microcompartment that functio

127 The carboxysome is a bacterial organelle found in all cyanob

128 The carboxysome is a protein-based organelle for carbon fixa

129 The cyanobacterial carboxysome is a proteinaceous microcompartment that pro

130 The carboxysome is believed to function as a microcompartmen

131 e also demonstrate that CO2 retention in the carboxysome is necessary, whereas selective uptake of HC

132 The number of carboxysomes is increased in 4 under high-CO2 conditions

133 We propose that carboxysome-like organelles help bacteria conserve certa

134 In S. enterica, this carboxysome-like structure (hereafter referred to as the

135 ny chemoautotrophs, products of the expanded carboxysome locus include CbbO and CbbQ, a member of the

136 hought to localize differentially within the carboxysome matrix.

137 eport the first structural insights into the carboxysome of Prochlorococcus, the numerically dominant

138 Prototypes of the BMCs are the carboxysomes of autotrophic bacteria.

139 These structures, typified by carboxysomes of cyanobacteria and many chemoautotrophs,

140 The first BMCs discovered were the carboxysomes of cyanobacteria.

141 al and cluster in the central cytoplasm, the carboxysomes of MED4 are smaller.

142 he organization of this lattice in beta-type carboxysomes of the freshwater cyanobacterium Synechococ

143 The alpha-carboxysome operon is embedded in a cluster of additiona

144 nzyme, cbbL and cbbS, are part of a putative carboxysome operon.

145 lyzing the genomic regions around alpha-type carboxysome operons, we characterize a protein that is c

146 s are found to co-occur near or within alpha-carboxysome operons.

147 or the transfer, regulation, and assembly of carboxysomes, or any of the myriad of functionally disti

148 skeletal protein ParA specifically disrupted carboxysome order, promoted random carboxysome segregati

149 re on average approximately 250 RuBisCOs per carboxysome, organized into three to four concentric lay

150 determined the crystal structure of an alpha-carboxysome PCD-like protein from the chemoautotrophic b

151 ent in PCD enzymes is disrupted in the alpha-carboxysome PCD-like protein.

152 At the optimal carboxysome permeability, contributions from CO2 scaveng

153 Transmembrane bicarbonate transporters and carboxysomes play key roles in accumulating bicarbonate

154 has been the inability to obtain homogeneous carboxysome preparations.

155 In this study, structure and function of carboxysomes purified from wild type Halothiobacillus ne

156 chemical analysis indicates that if they are carboxysome related, they are not functional, i.e., do n

157 More broadly, the reengineered carboxysomes represent a proof of concept for a domain f

158 nserved PCD-like protein, renamed here alpha-carboxysome RuBisCO assembly factor (or acRAF), is a nov

159 disrupted carboxysome order, promoted random carboxysome segregation during cell division, and impair

160 The carboxysome serves as an ideal model system for testing

161 recent progress in the re-engineering of the carboxysome shell and core to offer a conceptual framewo

162 the potential for gated transport across the carboxysome shell and reveals a new type of building blo

163 expression data, were used to identify a new carboxysome shell component, CsoS1D, in the genome of Pr

164 Genes coding for carboxysome shell components and the encapsulated protei

165 lysis suggests that CsoSCA exists within the carboxysome shell either as a homodimer or as extended f

166 We find selectivity at the carboxysome shell is not necessary; there is an optimal

167 The carboxysome shell is roughly 800 to 1400 angstroms in di

168 necessary; there is an optimal non-specific carboxysome shell permeability.

169 The most abundant carboxysome shell protein contains a single bacterial mi

170 nerate a population-level description of the carboxysome shell protein, RuBisCO, and CcmM isoform loc

171 e-dimensional crystal structures of multiple carboxysome shell proteins, revealing a hexameric unit a

172 he three-dimensional structures of hexameric carboxysome shell proteins, which self-assemble into mol

173 s inorganic carbon transporters and the beta-carboxysome shell proteins.

174 The model requires the carboxysome shell to be a major barrier to CO2 efflux an

175 ion levels, catalytic rates, permeability of carboxysome shell) for efficient carbon fixation.

176 ic building block for incorporation into the carboxysome shell, and the trimers further dimerize, for

177 racts with CsoCbbO and is a component of the carboxysome shell, the first example of ATPase activity

178 eptide is essential for interaction with the carboxysome shell.

179 plausible, preliminary atomic models for the carboxysome shell.

180 tic operon in Escherichia coli that produces carboxysome shells.

181 atalytic rates measured for CsoSCA in intact carboxysomes suggest that the protein shell acts as a ba

182 bacillus neapolitanus and Nitrobacter agilis carboxysomes suggest that the structures are either icos

183 The resulting streamlined carboxysomes support photosynthesis.

184 Photosystem II activity and carboxysome synthesis are lost in the tocopherol mutants

185 Although both strains possess carboxysomes that are polygonal and cluster in the centr

186 osahedral protein microcompartments known as carboxysomes that encapsulate multiple copies of the CO(

187 membrane-bound organelles of eukaryotes, in carboxysomes the interior of the compartment forms first

188 The carboxysome, the cyanobacterial BMC for CO(2) fixation,

189 d averaging of the computationally extracted carboxysomes to minimize the missing data effects.

190 any bacteria employ a protein organelle, the carboxysome, to catalyze carbon dioxide fixation in the

191 arboxysome function may be possessed by each carboxysome type.

192 These include the two carboxysome types and a divergent set of metabolosomes,

193 ns indicate that the possession of different carboxysome types does not significantly influence the p

194 The two carboxysome types have arisen through convergent evoluti

195 Interestingly, both carboxysome types showed a response to cytosolic Ci, whi

196 s a multimolecular structure that mimics the carboxysome used by some photosynthetic bacteria to fix

197 It was found that H. neapolitanus carboxysomes vary widely in size and mass as shown by cr

198 Surprisingly, no more than one partial carboxysome was ever observed per cell.

199 ferences between alpha-carboxysomes and beta-carboxysomes, we found that the two strains are remarkab

200 Here, fluorescently tagged carboxysomes were found to be spatially ordered in a lin

201 Because the isolated P. marinus MED4 carboxysomes were free from contaminating membrane prote

202 more definitively, purified H. neapolitanus carboxysomes were re-examined by cryo-electron tomograph

203 ll of a cellular microcompartment called the carboxysome, where it converts HCO(3)(-) to CO(2) for us

204 The central CO2-fixing machinery is the carboxysome, which is composed of an icosahedral protein

205 S2B isoform were capable of producing intact carboxysomes, while those with only CsoS2A were not.

206 e/oxygenase (RubisCO) sequestered within the carboxysome with sufficiently high concentrations of its

207 nthesis and subcellular localization of beta-carboxysomes within a model cyanobacterium, Synechococcu

208 Rubisco-containing microcompartments called carboxysomes within cells.

209 The results show that the addition of carboxysomes without other CCM components substantially

210 hereas selective uptake of HCO3 (-) into the carboxysome would not appreciably enhance energetic effi