ライフサイエンスコーパス: cathepsin L

1 lla spp. is a competitive inhibitor of human cathepsin L.

2 POMC consistent with proteolysis of POMC by cathepsin L.

3 expression of nuclear target genes RyR1 and cathepsin L.

4 n L because of its inhibitory action against cathepsin L.

5 three cathepsin Cs, one cathepsin B, and one cathepsin L.

6 ibility to proteolysis by endocytic protease cathepsin L.

7 a previously unreported biological role for cathepsin L.

8 nhibition with selectivity for inhibition of cathepsin L.

9 itors of the pH-sensitive endosomal protease cathepsin L.

10 0, RIG-G, and EMAPII and decreased MEF2D and cathepsin L.

11 he 20S proteasome and its 19S regulator, and cathepsin L.

12 64), an inhibitor of thiol proteases such as cathepsin L.

13 e acid-dependent lysosomal cysteine protease cathepsin L.

14 recombinant protein on the cysteine protease cathepsin L.

15 d 100-fold selective for cruzain relative to cathepsin L.

16 ail cleavage events mediated by the protease Cathepsin L.

17 t the small molecule is a mixed inhibitor of cathepsin L.

18 n and did not inhibit the cysteine protease, cathepsin L.

19 ed a non-elastase variant similar to that of cathepsin L.

20 antiviral action likely is the inhibition of cathepsin L, a cellular enzyme that is essential for the

21 inant CTLA-2beta helped us to determine that cathepsin L, a cysteine protease, is one of its targets

22 tified, including hydrolytic enzymes such as cathepsin L, a cysteine proteinase involved in lysis of

23 ideneamino]thiourea} acts later, by blocking cathepsin L, a host protease required for processing of

24 rict reovirus uncoating still express mature cathepsin L, a lysosomal protease required for virion di

25 , we assessed the effects of this microbe on cathepsin L, a protease that cleaves CDP into a form wit

26 Cells and mice lacking cathepsin L accumulate full-length Ctr1 and hyper-accumu

27 agocytophilum infection resulted in elevated cathepsin L activity and the proteolysis of CDP.

28 The intracellular localization of cathepsin L activity and topo I in necrotic cells was ex

29 ate that testican-1 is capable of modulating cathepsin L activity both in intracellular vesicles and

30 However, removal of both cathepsin B and cathepsin L activity completely abrogates disassembly an

31 For the ice stored fillets, the cathepsin L activity decreased significantly from 6 to 2

32 During necrosis, cathepsin L activity diffused from lysosomes into the cy

33 in macrophages, TLR9-GFP cleavage depends on cathepsin L activity in B cells.

34 There was also a significantly lower cathepsin L activity in the super-chilled fillets at 0h

35 These data demonstrate that increasing cathepsin L activity is a strategy used by A. phagocytop

36 ide evidence that NPC1(+) LE/Lys have higher cathepsin L activity than LE, with no detectable activit

37 Cathepsin L activity was 100-fold greater in Vero cells

38 ed in crowded and chilled salmon whereas the cathepsin L activity was found to be significantly affec

39 Furthermore, while cathepsin L activity was seen to be marginally important

40 ty in vitro and partially inhibits lysosomal cathepsin L activity within live APCs.

41 only endosomal requirement for SARS entry is cathepsin L activity, we tested and provide evidence tha

42 locking antibody increased the expression of cathepsin L activity.

43 PDK4 protein expression, and proteasome and cathepsin-L activity, and reduced muscle PDC activity.

44 p between the expression of cystatin E/M and cathepsin L and a direct relationship between the loss o

45 ry event involving a novel interplay between cathepsin L and alpha(3) integrin.

46 ated interplay between the cysteine protease cathepsin L and alpha(3) integrin.

47 blockade of other cysteine proteases such as cathepsin L and calpain, aminopeptidases, elastase, or m

48 s of cathepsin L in neuronal media, and both cathepsin L and cathepsin B were demonstrated to be impo

49 ted selective and potent inhibitors of human cathepsin L and cathepsin K in an in vitro assay of huma

50 Interactions of endopin 2C with cathepsin L and elastase were indicated by protease clea

51 Mechanistically, both glomerular cathepsin L and heparanase expression were reduced.

52 by analysis of I-A(b) mice deficient in both cathepsin L and invariant chain.

53 y that histone proteolysis, brought about by Cathepsin L and potentially other family members, plays

54 Markers of tumor invasion, cathepsin L and TGFbeta1, were overexpressed; calcium-de

55 vel substituents for the apolar S2 pocket of cathepsin L and was conducted entirely in a prospective

56 t, small GTPases, and the cysteine proteases cathepsin-L and -B.

57 The expressions of cathepsin-L and the muscle-specific E3 ligases MuRF-1 an

58 ceptor binding, and ability to be cleaved by cathepsins L and B.

59 by inhibits acid-dependent proteases such as cathepsins L and B.

60 cleavage profile was reproduced in vitro by cathepsins L and H and was inhibited by the cathepsin L

61 f F. hepatica, FhCL1 and FhCL2, and of human cathepsins L and K (Ki = 0.4-27 nm).

62 itors docked within the active sites of both cathepsins L and K have rationalized the observed select

63 s selectivity toward FhCL1, FhCL2, and human cathepsins L and K.

64 activity of other cysteine proteases such as cathepsins L and S at 37 degrees C.

65 In contrast to the joint requirement for cathepsins L and S for TLR9 cleavage in macrophages, TLR

66 to cleavage by the NOX2-controlled cysteine cathepsins L and S in a redox-dependent manner.

67 selective for cathepsin K when compared with cathepsins L and S, with the Ki values in the 10-30 nM r

68 extracts, and pure cathepsins, we identified cathepsins L and Z as the lysosomal cysteine proteases t

69 RNAi for cathepsins L and Z in different cell lines and adult mou

70 1-dependent haptotaxis, whereas induction of cathepsins-L and -B promotes matrix invasion.

71 is, increased expression of the tumor marker cathepsin L, and a high degree of invasiveness as tested

72 killing, regulation of the hydrolytic enzyme cathepsin L, and for coordination and trafficking of MHC

73 tion by three classes of proteases: plasmin, cathepsin L, and matrix metalloproteinases (MMP-2 and MM

74 e increased mRNA levels of atrogin-1, MuRF1, cathepsin L, and/or Bnip3 and inhibited muscle fiber atr

75 Nine genes, PDGF A, Cathepsin L, annexin A1, Mm.112139, Est2 repressor facto

76 Isolated podocytes from mice lacking cathepsin L are protected from cell puromycin aminonucle

77 finding suggests that factors in addition to cathepsin L are required for efficient intracellular pro

78 represent the basis for a novel function of cathepsin L as a cell survival molecule responsible for

79 These findings demonstrate cathepsin L as a distinct cysteine protease pathway for

80 We map the sites of H3 cleavage and identify Cathepsin L as a protease responsible for proteolyticall

81 Cathepsin L, B, and D cleavage sites in mouse Tg were pr

82 or but not by an inhibitor effective against cathepsins L, B, and S.

83 , NM23-H1, is degraded by lysosomal cysteine cathepsins (L,B), which directly cleave NM23-H1.

84 Here, we identify the cathepsin L/B endolysosomal proteases functioning in a d

85 ate that the combination of cisplatin with a cathepsin L/B inhibitor enhances cisplatin uptake and ce

86 The compound also displayed cathepsin L/B selectivity of >700-fold and was nontoxic

87 Optimization of cathepsin L binding by the combination of the P3 naphthy

88 Inhibitors of cathepsin L blocked infection by SARS-CoV and by a retro

89 RS-S with ACE2 or the enzymatic functions of cathepsin L but prevents fusion of the viral membrane wi

90 Cathepsin L, but not cathepsin B, is an important contri

91 LECs express invariant chain and cathepsin L, but not H2-M, suggesting that they cannot l

92 than caspases have shown that inhibition of cathepsin L, but not proteasome or cathepsin B, was resp

93 cleavage by the endosomal/lysosomal protease cathepsin L, but the route of Hendra virus F following i

94 cysteine protease activity was identified as cathepsin L by affinity labeling with an activity-based

95 whereas thymic cortical epithelial cells use cathepsin L (Cat L) for invariant chain degradation and

96 the elastinolytic and collagenolytic enzyme cathepsin L (Cat L) in human atherosclerotic lesions sug

97 Cathepsin L (Cat L) is a cysteine protease that can prot

98 immune response, we generated mice that lack cathepsin L (Cat L) on the autoimmune diabetes-prone NOD

99 rther, processing of the lysosomal proteases cathepsin L (CatL) and CatB into their fully active, mat

100 h the cleavage of GP by proteases, including cathepsin L (CatL) and/or CatB, in the endosome or cell

101 Here we demonstrate a role for cathepsin L (CatL) cleavage of Ebola virus GP in the gen

102 how that mice lacking the endosomal protease cathepsin L (catL) have greatly reduced numbers of V(alp

103 e confirmed roles for cathepsin B (CatB) and cathepsin L (CatL) in Ebola virus glycoprotein (GP)-medi

104 cathepsin B (CatB) and an accessory role for cathepsin L (CatL) in EboV GP-dependent entry.

105 Cathepsin S (catS) and cathepsin L (catL) mediate late stages of invariant chai

106 ity complex (MHC), invariant chain (Ii), and cathepsin L (CatL) molecules involved in thymocyte-posit

107 aa segment that binds to the active site of cathepsin L (CatL), a lysosomal cysteine protease involv

108 on factor to promote expression of cytosolic cathepsin L (CatL).

109 ivation of a lysosomal and nuclear protease, cathepsin L, causes a global redistribution of epigeneti

110 ings were achieved by coexpression of PE and cathepsin L cDNAs in PC12 cells with analyses of PE-deri

111 The cysteinal lysosomal proteases, cathepsin L (CL) and cathepsin S (CS), have been shown t

112 tive endosomal compartments, suggesting that cathepsin L cleavage occurs in early endosomes.

113 overed a small molecule that can inhibit the cathepsin L cleavage of all viral peptides with minimal

114 to identify small molecules that can prevent cathepsin L cleavage of viral glycoproteins derived from

115 age, and these amino acids included a likely cathepsin L cleavage site.

116 he aforementioned viruses, which contain the cathepsin L cleavage site.

117 ough dynamin self-assembly, are resistant to cathepsin L cleavage.

118 Structure-assisted modeling of the cathepsin L-cleaved ZEBOV-GP revealed that cleavage remo

119 2C that demonstrates selective inhibition of cathepsin L compared to papain or elastase.

120 Processing of cathepsin L (CPL) in these mutants was deficient only wh

121 (now Ctsl(nkt)) comprises a deletion in the cathepsin L (Ctsl) gene.

122 unprecedented role for the cysteine protease Cathepsin L (CTSL) in the degradation of 53BP1.

123 The cysteine protease cathepsin L (CTSL) is often thought to act as a tumor pr

124 We found that the T cell-expressed protease cathepsin L (CTSL) processed C3 into biologically active

125 activated by HDAC and an endogenous protease cathepsin L (CTSL) that remove the acetyl group first an

126 re, we demonstrate that BRCA1 loss activates cathepsin L (CTSL)-mediated degradation of 53BP1.

127 but were again sex specific for three genes: cathepsin-L (CtsL), matrix metalloproteinase-14 (MMP-14)

128 was a potent inhibitor of the major secreted cathepsin L cysteine proteases of F. hepatica, FhCL1 and

129 Our data indicate the cathepsin L defect in CD4+ T cell selection is haplotype

130 lock in invariant chain cleavage we analyzed cathepsin L-deficient mice expressing the I-A(q) haploty

131 bitor Z-Phe-Tyr(t-Bu)-diazomethyl ketone and cathepsin L-deficient mouse embryo fibroblasts resulted

132 de-induced cell migration was slowed down in cathepsin L-deficient podocytes and by the preservation

133 ling acts, in part, through calcineurin- and cathepsin L-dependent cleavage of synaptopodin, a regula

134 13) report the discovery of an intracellular cathepsin-L-dependent C3 activation pathway.

135 Late endosomal markers and the lysosomal cathepsin L do not colocalize with P. gingivalis 381.

136 vesicles, demonstrated by colocalization of cathepsin L-DsRed fusion protein with enkephalin and chr

137 3631927) was tested as an inhibitor of human cathepsin L (EC 3.4.22.15) and as an entry blocker of se

138 m SID 26681509), a potent inhibitor of human cathepsin L (EC 3.4.22.15) with an IC(50) of 56 nM, was

139 provide evidence that a lysosomal protease, cathepsin L, exists in a previously unsuspected isoform

140 Cathepsin L expression and activity were induced in podo

141 mycin aminonucleoside treatment up-regulates cathepsin L expression in podocytes in vivo as well as e

142 The functional significance of cathepsin L expression was underscored by the observatio

143 Virus infection induced cellular cathepsin L expression while reducing the levels of othe

144 cent phenotype and a marked up-regulation of cathepsin-L expression.

145 Cruzain is a member of the papain/cathepsin L family of cysteine proteases, and the major

146 newly defined secretory vesicle function of cathepsin L for biosynthesis of active enkephalin opioid

147 These viruses depend on cathepsin L for entry into their target cells.

148 region is consistent with the specificity of cathepsin L for hydrophobic residues in the P2 position

149 The importance of cathepsin L for infection of other cell types is unknown

150 novel cellular role of the cysteine protease cathepsin L for producing the (Met)enkephalin peptide ne

151 Key findings from this study show that cathepsin L functions as a major proteolytic enzyme for

152 We describe a mutant of pro-cathepsin L fused to YFP that no longer targets to the l

153 ess conditions was associated with increased cathepsin L gene expression, which contributes to increa

154 Finally, in cathepsin L gene knockout mice, [Met]enkephalin levels i

155 Although cathepsin L generated the requisite TLR9 cleavage produc

156 ndrial stress-induced expression of RyR1 and cathepsin L genes.

157 endosomal cysteine proteases cathepsin B and cathepsin L greatly reduce MHV-2 spike-mediated entry, w

158 virus entry requires the endosomal protease cathepsin L; however, it was also found that infection o

159 uvate kinase, Glut4), oncogenesis (TGFbeta1, cathepsin L, IGFR1, melanoma antigen) and apoptosis (Bcl

160 y of acoustic dispensing by delivering human cathepsin L in a drop-on-drop fashion into individual 50

161 To further examine the putative role of cathepsin L in bone resorption, we have evaluated select

162 The reduction in active cathepsin L in inhibitor-treated cells correlated well w

163 r hand, IL-1alpha treatment raised levels of cathepsin L in neuronal media, and both cathepsin L and

164 Expression of cathepsin L in pituitary AtT-20 cells resulted in increa

165 well as expression and enzymatic activity of cathepsin L in podocytes in vitro.

166 Thus, we propose a novel role for cathepsin L in regulating positive selection by generati

167 Similar localization of a related cathepsin L in the filarial nematode Onchocerca volvulus

168 ing P. gingivalis 381 lacks BiP but contains cathepsin L in the presence of wortmannin.

169 erized biological role for secretory vesicle cathepsin L in the production of [Met]enkephalin, an end

170 se findings demonstrate a prominent role for cathepsin L in the production of ACTH, beta-endorphin, a

171 In vivo studies also showed that cathepsin L in vivo was colocalized with enkephalin.

172 e results implicate cathepsins, particularly cathepsin L, in the cleavage of topo I during necrosis.

173 or virus-specific differences in the role of cathepsin L, including cooperation with cathepsin B.

174 Deletion of CTSB reduced and deletion of cathepsin L increased intracellular trypsin activation.

175 In the cathepsin L inhibition assay, the oxocarbazate caused a

176 All together, these findings suggest that cathepsin L inhibition in drug-resistant cells facilitat

177 on of the underlying mechanism revealed that cathepsin L inhibition resulted in the alteration of int

178 nhibitors (FK506 and cyclosporine A) and the cathepsin L inhibitor E64 all inhibited protamine sulfat

179 cathepsins L and H and was inhibited by the cathepsin L inhibitor Z-FY-CHO.

180 nfection of both L929 cells treated with the cathepsin L inhibitor Z-Phe-Tyr(t-Bu)-diazomethyl ketone

181 When tested against cathepsin L, inhibitor I and all its polymer conjugates

182 The potent selective cathepsin L inhibitors (K(i) = 0.0099, 0.034, and 0.27 n

183 hibitors of the homologous cysteine protease cathepsin L is detailed.

184 Testican-1 inhibition of cathepsin L is independent of its chondroitin sulfate ch

185 The expressions of involucrin, loricrin, and cathepsin L is initially increased by day 19 but subsequ

186 demonstrate here that the cellular protease cathepsin L is involved in converting the Hendra virus p

187 SARS-CoV membrane fusion and indicates that cathepsin L is sufficient to activate membrane fusion by

188 These data confirm that cathepsin K and not cathepsin L is the major protease responsible for human

189 ry into murine fibroblasts and indicate that cathepsin L is the primary mediator of reovirus disassem

190 We show here that secretory vesicle cathepsin L is the responsible cysteine protease of chro

191 ted dipeptide aldehyde with activity against cathepsins L (K(i) = 0.052 nm) and K (K(i) = 1.57 nm) wa

192 and exhibited notable selectivity over human cathepsins L, K, and B.

193 Specifically, cathepsin L knock-out mice showed major decreases in ACT

194 In cathepsin L knockout cells, the levels of trimethylated

195 ggesting cortical thymic epithelial cells in cathepsin L knockout mice express an altered peptide rep

196 ric kidney disease, induction of cytoplasmic cathepsin L leads to cleavage of dynamin at an evolution

197 exosite 1 or 2 with analogous residues from cathepsin L led to a 75 and 43% loss in the elastolytic

198 Treatment of wt mice with an inhibitor of cathepsin L led to amelioration of reovirus infection.

199 Rhodesain, a cathepsin L-like cysteine protease of T. brucei rhodesie

200 ain (also brucipain, trypanopain), the major cathepsin L-like cysteine protease of T. brucei, genomic

201 acterized Ce-cpl-1, a Caenorhabditis elegans cathepsin L-like cysteine protease.

202 t T. b. gambiense displayed higher levels of cathepsin L-like cysteine proteases, we investigated whe

203 nzene (K11777), an irreversible inhibitor of cathepsin L-like cysteine proteases.

204 human malaria, there are four members of the cathepsin L-like family of cysteine proteases.

205 ease for proline and leucine residues into a cathepsin L-like preference for bulky aromatic residues.

206 phatase (TgVP1), a vacuolar proton ATPase, a cathepsin L-like protease (TgCPL), an aquaporin (TgAQP1)

207 the major parasite-secreted proteases and/or cathepsin L-like proteases of its host.

208 The cathepsin L-like S2 specificity of the mutant protein an

209 ed the S2 pocket of human cathepsin K into a cathepsin L-like subsite.

210 ffect interactions with the ACE2 receptor or cathepsin L-mediated activation of SARS-S-driven membran

211 oad-spectrum small molecule that could block cathepsin L-mediated cleavage and thus inhibit the entry

212 evels, 53BP1 loss is caused by activation of cathepsin L-mediated degradation of 53BP1 protein.

213 raction, in turn, protects synaptopodin from cathepsin L-mediated degradation.

214 ediated Akt/FOXO inhibition, and blunting of cathepsin-L-mediated lysosomal protein breakdown.

215 Cathepsin L mediates invariant chain processing in corti

216 Concordantly, cathepsin L mediates reovirus disassembly more efficient

217 , c-Jun, Sp1, Sin, and tomosyn and decreased cathepsin L, Mre11, and topoisomerase II alpha.

218 ficantly reduce the LPS-mediated increase in cathepsin-L mRNA expression and enzyme activity by 43% (

219 uscle PDK4, muscle atrophy F-box (MAFbx) and cathepsin-L mRNA expression, increased PDK4 protein expr

220 Cathepsin-L mRNA was 2-fold higher (P < 0.01), whilst ca

221 ased muscle PDK4 mRNA and protein, MAFbx and cathepsin-L mRNA, increased activity of PDC and reduced

222 Production of [Met]enkephalin by cathepsin L occurred by proteolytic processing at dibasi

223 olysis of D-EA virions by endocytic protease cathepsin L occurred with faster kinetics than proteolys

224 r cells and posttranslationally activated by cathepsin L of tubular origin, sustains continuous activ

225 These results demonstrate that either cathepsin L or cathepsin B is required for reovirus entr

226 SVPs) with either of the endocytic proteases cathepsin L or cathepsin D demonstrated that an isolated

227 ents of the NLRP3 inflammasome, cathepsin B, cathepsin L or IL-1 molecules.

228 ct in cell-mediated immunity in mice lacking cathepsin L or S.

229 carboxamide led to increased selectivity for cathepsin L over cathepsin K.

230 lso resulted in an increased selectivity for cathepsin L over cathepsin K.

231 i.e. MAFbx (P < 0.001), MuRF-1 (P < 0.001), cathepsin-L (P < 0.05), PDK2 (P < 0.05) and PDK4 (P < 0.

232 Endopin 2C formed SDS-stable complexes with cathepsin L, papain, and elastase that are typical of se

233 ent protein provide strong evidence that the cathepsin L phosphorylation signal is a simple structure

234 ides in vivo evidence that cysteine protease cathepsin L plays a critical role in hair follicle morph

235 signal sequence of endopin 2C, like that of cathepsin L, predicts their colocalization to subcellula

236 Additionally, we found that purified human cathepsin L processed immunopurified Hendra virus F(0) i

237 Cathepsin-L promotes injury through an antiapoptotic eff

238 Podocyte alkalinization reduces cytosolic cathepsin L protease activity and protects the podocyte

239 mbryonic lethality caused by the loss of the cathepsin L protease, indicating that the accumulation o

240 es to cathepsin L which dramatically reduced cathepsin L protein expression and enzyme activity.

241 ture GILT and in regulating levels of mature cathepsin L protein in B cells.

242 Despite the presence of mature cathepsin L protein, no enzyme activity could be detecte

243 The requirement for cathepsin L proteolysis identifies a previously uncharac

244 tional changes in S glycoprotein followed by cathepsin L proteolysis within endosomes.

245 of IL-10, whereas tumor necrosis factor and cathepsin L release was reduced, further confirming pola

246 Expression of cathepsin L resulted in highly increased cellular levels

247 cells with CLIK-148, a specific inhibitor of cathepsin L, resulted in reduced production of ACTH and

248 -depleted cells stably expressing anti-sense cathepsin L RNA, TGFbeta1 RNA, or treated with specific

249 ivator in up-regulating the transcription of Cathepsin L, RyR1, and Glut-4, the target genes of stres

250 previously established stress response genes Cathepsin L, RyR1, and Glut4.

251 0 recognized by three major human proteases (cathepsins L, S, and D) important for antigen processing

252 r and plasminogen, the cysteine proteinases, cathepsins L, S, and K, and the matrix metalloproteinase

253 hly homologous cysteine proteases, including cathepsins L, S, and V.

254 llagenous barriers independently of plasmin, cathepsins L, S, or K, MMP-2, or MMP-9.

255 rier function through influencing macrophage cathepsin L secretion, thus reducing activation of the g

256 Cathepsin L shRNA-expressing Vero cells transfected with

257 it did not rely on nuclear factor kappaB or cathepsin L signaling.

258 hat lack the cathepsin L site, or render the cathepsin L site inaccessible through dynamin self-assem

259 Dynamin mutants that lack the cathepsin L site, or render the cathepsin L site inacces

260 At neutral pH, testican-1 also stabilizes cathepsin L, slowing pH-induced denaturation and allowin

261 cal approach, through cell transfection with cathepsin L small interfering RNA, also strongly reverse

262 Thus, our data show that cathepsin L stabilizes epigenetic heterochromatin marker

263 Expression of exogenous cathepsin L substantially enhanced infection mediated by

264 emonstrated that p21/WAF1 is a substrate for cathepsin L, suggesting that inhibition of this enzyme m

265 We evaluated E64 resistance and in vitro cathepsin L susceptibility of these viruses and found th

266 haracterized the T. gondii cysteine protease cathepsin L (TgCPL), one of five cathepsins found in the

267 ed 7- to 151-fold greater selectivity toward cathepsin L than papain and cathepsins B, K, V, and S wi

268 slow-binding, reversible inhibitor of human cathepsin L that blocked SARS-CoV and Ebola pseudotype v

269 ed nuclear enzymatic activity of a protease (cathepsin L) that has been shown to cleave full-length C

270 action of the inhibitors with intracellular cathepsin L, the activity-based probe biotin-Lys-C5 alky

271 pite close sequence homology to the protease cathepsin L, the silicateins seem to exhibit no signific

272 After a 4-h preincubation with cathepsin L, this compound became even more potent, demo

273 that the defect in positive selection in the cathepsin L-/- thymus is specific for CD4+ T cells that

274 including cysteine proteases cathepsin B and cathepsin L, tissue inhibitor of matrix metalloproteinas

275 redominantly expressed by melanoma cells and cathepsin L to be predominantly expressed by the tumor-a

276 s with increasing specificity, we identified cathepsin L to be the protease responsible for cleavage.

277 enzymatic activity of the cysteine protease cathepsin L to infect ACE2-expressing cells.

278 n, FhHDM-1 is rapidly processed by lysosomal cathepsin L to release a short C-terminal peptide (conta

279 Functional localization of cathepsin L to the regulated secretory pathway was demon

280 ng of the precursor forms of cathepsin D and cathepsin L to their mature, lysosomal forms, which coin

281 okinin (CCK) increased the activity of CTSB, cathepsin L, trypsin, chymotrypsin, and caspase 3 in viv

282 In contrast, cathepsin L, V, B, and S revealed no collagenase activit

283 CA, family C1) papain, bromelain, and human cathepsins L, V, K, S, F, B, and five proteases of paras

284 In addition, cathepsin L was able to cleave the G protein in Vero cel

285 Cathepsin L was detected in some bronchial epithelial, e

286 Consistent with this finding, expression of cathepsin L was detected in the giant trophoblast cells

287 In contrast, cathepsin L was only partially colocalized with the lyso

288 ispensable for establishment of viremia, but cathepsin L was required for maximal reovirus growth in

289 Cathepsin-L, water soluble and total protein components

290 Using this mutant pro-cathepsin L, we found that components of the mammalian E

291 of the 20S and 26S proteasomes, calpain and cathepsin L, were measured in the triceps surae muscles

292 We next designed shRNA oligonucleotides to cathepsin L which dramatically reduced cathepsin L prote

293 olytic activity, the structurally homologous cathepsin L, which shares a 78% amino acid sequence, has

294 ellular studies showed the colocalization of cathepsin L with [Met]enkephalin in secretory vesicles o

295 Blocking the action of cathepsin L with a chemical inhibitor or small interferi

296 elective, and competitive inhibitor of human cathepsin L with a K(i) = 0.43 nM.

297 nt endopin 2C showed effective inhibition of cathepsin L with a stoichiometry of inhibition (SI) of 1

298 rescence microscopy showed colocalization of cathepsin L with beta-endorphin and alpha-MSH in the int

299 Furthermore, expression of cathepsin L with PE resulted in increased amounts of nic

300 nhibitor of the lysosomal cysteine protease, cathepsin L, with a Ki of 0.7 nM, but it does not inhibi