1 to a promoter on a clock controlled gene-
2 (
ccg-2).
2 ions that affect the regulation of ccg-1
and ccg-2 by the frq-oscillator.
3 expression of the morning-specific ccg-1
and ccg-2 genes.
4 ion levels and rhythmicity of both ccg-1
and ccg-2.
5 Coexpression of pre-2
and ccg-4 in the mat A background leads to self-attraction a
6 Correlations
between ccg abundance from RNA-Seq, the degree of ccg-promoter a
7 Rhythms in
both ccg-7 mRNA accumulation and CCG-7 (GAPDH) activity are o
8 he expression levels and rhythmicity of
both ccg-1 and ccg-2.
9 ee of ccg-promoter activation as reported
by ccg-promoter-luciferase fusions, and binding of WCC as m
10 ing that half of the new ccgs, including
cmt(
ccg-12), are not transcriptionally induced by developmen
11 In addition,
eas (
ccg-2) is positively regulated by light and transcripts
12 The Neurospora crassa
eas (
ccg-2) gene, which encodes a fungal hydrophobin, is tran
13 uired for clock-regulated expression of
eas (
ccg-2).
14 was found in the promoter region of the
eas (
ccg-2) gene, which encodes a fungal hydrophobin.
15 cient to confer clock regulation on the
eas (
ccg-2) gene.
16 Separate regions of the
eas (
ccg-2) promoter involved in light induction and developm
17 ex regulation, deletion analyses of the
eas (
ccg-2) promoter were carried out to localize the cis-act
18 genes, including the well-characterized
eas(
ccg-2) gene.
19 ing that other proteins directly control
eas(
ccg-2) rhythmic expression.
20 hich in turn activates the expression of
eas(
ccg-2) at specific times of the day.
21 tely eliminate, rhythmic accumulation of
eas(
ccg-2) mRNA in vivo, whereas deletion of the entire ACE
22 in circadian regulation of expression of
eas(
ccg-2).
23 tes for the protein factors that promote
eas(
ccg-2) rhythms exist within the 68 bp ACE.
24 Dissection of the
eas(
ccg-2) gene promoter previously localized a 68 bp sequen
25 OP1-1 (circadian output pathway derived
from ccg-1), that has altered expression of ccg-1 mRNA, but n
26 d analysis of a novel clock-controlled
gene,
ccg-7, showing similarity to glyceraldehyde-3-phosphate
27 ed, the isolation of clock-controlled
genes (
ccgs) was pioneered in Neurospora where circadian output
28 ythmic expression of clock-controlled
genes (
ccgs).
29 We identified 145 clock-controlled
genes (
ccgs).
30 to identify six new clock-controlled
genes (
ccgs).
31 ng as is the case with previously
identified ccgs.
32 tivity lags several hours behind the peak
in ccg-7 mRNA accumulation in the late night.
33 ) we estimated that >94% of the variation
in ccg-2 expression was stochastic (as opposed to experimen
34 Sequencing of the ends of the
new ccg cDNAs revealed that ccg-12 is identical to N. crassa
35 as supported by finding that half of the
new ccgs, including cmt(ccg-12), are not transcriptionally i
36 altered expression of ccg-1 mRNA, but
normal ccg-2 expression levels.
37 ate a repressor of ccg-1 and an activator
of ccg-2 in two independent pathways, since in our selectio
38 tes a repressor of ccg-1 and an activator
of ccg-2.
39 en ccg abundance from RNA-Seq, the degree
of ccg-promoter activation as reported by ccg-promoter-luci
40 from ccg-1), that has altered expression
of ccg-1 mRNA, but normal ccg-2 expression levels.
41 for mutations that affect the regulation
of ccg-1 and ccg-2 by the frq-oscillator.
42 not appear to simply regulate a repressor
of ccg-1 and an activator of ccg-2 in two independent pathw
43 the frq-oscillator regulates a repressor
of ccg-1 and an activator of ccg-2.
44 dian transcription factor WCC to a subset
of ccg promoters, including those of many transcription fac
45 y correlated with the temporal regulation
of ccgs.
46 y due to heterologous expression of pre-2
or ccg-4 behave normally in crosses with opposite mating-ty
47 44 controls the circadian expression of
some ccgs.
48 rhythmic expression of the morning-
specific ccg-1 and ccg-2 genes.
49 In frq-null or wc-1 mutant
strains,
ccg-1 mRNA levels fluctuate near peak levels over the co
50 That ccg-7 encodes GAPDH was confirmed by demonstrating that
51 Together with our previous observation
that ccg-7 mRNA is not developmentally regulated, we show tha
52 the ends of the new ccg cDNAs revealed
that ccg-12 is identical to N. crassa cmt encoding copper met
53 not developmentally regulated, we show
that ccg-7 is not induced by environmental stresses such as g
54 The ccgs peaked in mRNA accumulation at all phases of the da
55 between the circadian oscillator(s) and
the ccgs.
56 The predicted or known functions of
the ccgs demonstrate that the clock contributes to a wide ra
57 Transcripts specific for each of
the ccgs preferentially accumulate during the late night to
58 Although the period of rhythm of most of
the ccgs was found to depend on the well characterized frequ
59 These ccgs accumulate mRNA rhythmically with a circadian perio
60 ized frequency (FRQ)-based oscillator,
three ccgs appeared to have a rhythm that was significantly sh
61 although circadian activation is critical
to ccg rhythmicity, posttranscriptional regulation plays a
62 k levels over the course of the day,
whereas ccg-2 mRNA remains at trough levels.