ライフサイエンスコーパス: cell culture

1 te threefold more reactive oxygen species in cell culture.

2 g the production of the final virus stock in cell culture.

3 ted by rs7688285 in brain tissue, as well as cell culture.

4 1 single and double infection experiments in cell culture.

5 inhibited RNase H without inhibiting HIV in cell culture.

6 otency and selectivity, both in vitro and in cell culture.

7 s similar to those of the wild-type virus in cell culture.

8 -electron microscopy, molecular biology, and cell culture.

9 from epithelial and immune cells expanded in cell culture.

10 ally diverse range of therapeutics in cancer cell culture.

11 ial activity toward neuronal phagocytosis in cell culture.

12 ytoplasm and persistent viral replication in cell culture.

13 complexes (ICs) with nucleosomal Ags during cell culture.

14 ng, biomolecule delivery, cell delivery, and cell culture.

15 (TCR) sequencing approach without long-term cell culture.

16 gregation and forms a 3D matrix suitable for cell culture.

17 s hydrogels are proposed to support in vitro cell culture.

18 nts against prototypic poxvirus infection in cell culture.

19 and SMN-deficient motor-neuron-like cells in cell culture.

20 nhibits human cytomegalovirus replication in cell culture.

21 of the cellular microenvironment in primary cell culture.

22 ociated with reduced viability and growth in cell culture.

23 onstration of aptamer-specific resistance in cell culture.

24 itope (EDE) can neutralize ZIKV infection in cell culture.

25 challenging to grow HCV clinical isolates in cell culture.

26 We recapitulated these results in cell cultures.

27 d extensive assay replicates in two types of cell cultures.

28 y controls (n = 25) were cultured as primary cell cultures.

29 ed upon TNF-alpha stimulation of endothelial cell cultures.

30 t ATM is proviral in the context of infected cell cultures.

31 ular vs. extracellular localization even for cell cultures.

32 muM) inhibited the growth of ovarian cancer cell cultures.

33 tor cells were evaluated with in vitro human cell cultures.

34 utonomous as it was also observed in primary cell cultures.

35 rry out the cross-coupling chemistry in live cell cultures.

36 gene expression when compared to standard 2D cell cultures.

37 ucleocapsid protein and its possible link to cell culture adaptation.

38 have been derived from naturally occurring, cell culture-adapted, or genetically modified live atten

39 Cell biology differs between traditional cell culture and 3-dimensional (3-D) systems, and is mod

40 nt study, a novel platform for conducting 3D cell culture and analyzing cell viability has been devel

41 n hydrolysates or peptides were tested using cell culture and animal models, while a few involved cli

42 In cell culture and animal studies, these effects alter the

43 ical challenges to be met before traditional cell culture and animal testing become obsolete.

44 attenuated in their ability to propagate in cell culture and are cleared faster from the murine geni

45 are mainly based on the two-dimensional (2D) cell culture and are limited by the difficulty of simula

46 NAs are down-regulated upon WT1 depletion in cell culture and developing kidney mesenchyme.

47 eck/Gpr124 stimulation of Wnt7a signaling in cell culture and impairs CNS angiogenesis.

48 these viruses evolved during replication in cell culture and in experimentally infected macaques.

49 lation of regulators of this pathway both in cell culture and in mice demonstrated robust regulation

50 role in controlling HSV-1 infection both in cell culture and in mice.

51 factor was found to be severely impaired by cell culture and in vivo assays, indicating that the bal

52 Using in vitro cell culture and in vivo mouse models, we showed that CO

53 n vitro data, along with those from previous cell culture and in vivo studies by others, suggest that

54 Due to limitations of 2D cell culture and mouse models, there is a need to develo

55 ant with the decrease of CHK1 levels both in cell culture and mouse rhadomyosarcoma xenografts.

56 s role in GOF p53 dependency, experiments in cell culture and mouse xenografts demonstrated that inhi

57 Experience with human iPS cell culture and sorting via FACS will be of benefit for

58 dification inhibits apoptosis in tissues and cell culture and that lowering O-GlcNAcylation induces c

59 efficient, requires additional operation for cell culture and therefore, is not compatible with point

60 Thus, these cell culture and whole animal studies demonstrate a role

61 NST cells inhibits cell growth both in human cell culture and xenograft mice by increasing apoptosis.

62 tion over current methods of patient-derived cell culture and xenograft models.

63 y enhancer-99), which activates autophagy in cell cultures and animal models.

64 was added by extracting RNA from independent cell cultures and degrading particular samples.

65 rophoblast cells from both differentiated TS cell cultures and dissected mouse placentae resulted in

66 oduction directly in oligodendrocyte lineage cell cultures and exerted robust protection on oligodend

67 the consequences of the p.A211V mutation in cell cultures and in the CNS of a mutant mouse.

68 cked CFTR chloride conductance in epithelial cell cultures and intestine after cAMP agonists, cholera

69 ues are beginning to supplant traditional 2D cell cultures and preclinical animal studies that have h

70 es and potent cytotoxicities in human cancer cell cultures and reduced lethality in an animal model.

71 On the basis of studies in cell cultures and slice preparations, it is hypothesised

72 We infected nonhuman primate cell cultures and then crab-eating macaques with either

73 itochondria, resulting in potent activity in cell cultures and tumor xenografts in mice.

74 e demonstrate that RPE degeneration in human-cell-culture and mouse models is driven by a noncanonica

75 diagnostic format, neutralizes influenza in cell culture, and completely protects mice when given as

76 cs similar to those of wild-type R. typhi in cell culture, and stably maintain the plasmid and GFPuv

77 localize hundreds of metabolites in tissues, cell cultures, and agar plates with cellular resolution,

78 f testable predictions emerging for tissues, cell cultures, and even stem cell-based tissue regenerat

79 te infectious virus rely on in vivo or basic cell culture approaches, which prohibit rapid results an

80 We conclude that HCE cell cultures are highly susceptible to infection wherea

81 igh viability and suitable for primary tumor cell culture, are comprehensively characterized by in si

82 nstrated inhibition of IHNV by LJ001 both in cell culture as well as in live fish.

83 lso, cell orientation had a dramatic effect; cells cultured as epithelia exhibited much greater bindi

84 SAR analysis, using cell culture assays, led to the discovery of a series of

85 Using biochemical and cell culture assays, we identified HSPB7 as an actin fil

86 vitro-differentiated human nasal epithelial cells cultured at air-liquid interface.

87 that is based on pre-enrichment of virus in cell culture before search for the viral genome and vira

88 began many decades ago, when the pioneers of cell culture began to ask questions we still ask today:

89 itutions in its genome during replication in cell culture but that evolution in macaques was limited.

90 ndamentally reliant on high-quality adherent cell culture, but current methods to cryopreserve cells

91 that UL21 is dispensable for replication in cell cultures, but a recent report on HSV-2 challenges t

92 ntracellular calcium levels upon addition to cell culture, calcium release can be determined in these

93 During cell culture, changes in recombinant mRNA translation we

94 In cell culture, CnnT targets to the mitochondrial surface,

95 results indicate that EPCR is a reliable and cell culture-compatible marker of UM171-expanded human c

96 upled an efficient method to establish tumor cell cultures, conditional reprogramming (CR), with a ra

97 irradiance and radiant exposure, as well as cell culture conditions (e.g., serum concentration, cell

98 le and retains its attenuated genotype under cell culture conditions that readily select for reversio

99 ins, which lack antimicrobial activity under cell culture conditions, only inhibit macrophage activat

100 51 requires TCTP in MCF-7 cells under normal cell culture conditions.

101 cids, most nanoparticles have been tested in cell culture contexts that do not recapitulate systemic

102 for cellular resistance to nab-paclitaxel in cell culture correlated with a loss of Cav-1 expression.

103 Primarily these methods comprise single B-cell culture coupled to high-throughput neutralization s

104 ular migration by scratch-wounding confluent cell cultures, culturing under subconfluent conditions,

105 Cell-culture-derived HCV particles were produced from Hu

106 we explored the use of closed and miniature cell culture device for biomanufacturing patient specifi

107 t methods for inducing Wnt signaling in stem cell cultures do not control the spatial presentation of

108 s resulted in attenuation of virus growth in cell culture due to a reduction in assembly of mature vi

109 ugh the European Collection of Authenticated Cell Cultures (ECACC).

110 side biomimetic hydrogels that supplied a 3D cell culture environment.

111 MLN cells cultured ex vivo and treated with PalC had reduced

112 pluripotent stem cells for large scale stem cell culture expansion and differentiation platforms.

113 ming and erasing mechanical memory following cell culture expansion protects MSCs from fibrogenesis i

114 In the cell culture experiment, HeLa cells were simultaneously

115 Further in vitro cell culture experiments and gene expression analysis re

116 Biochemistry textbooks and cell culture experiments seem to be telling us two diffe

117 Furthermore, cell culture experiments showed a positive association b

118 Cell culture experiments showed that ApoA-IV improved gl

119 The results from our immunoprecipitation and cell culture experiments showed that MIG-6 inhibited pho

120 performed in vitro glycosylation assays and cell culture experiments to compare the activities and s

121 re room by personnel with standard mammalian cell culture expertise.

122 Similar to the primary tumor, tumor cell cultures expressed very high levels of the Myc prot

123 one) during days 16 to 30 followed by single-cell culture for 5 days on Matrigel mattress.

124 EAC cells cultured for 2 months with a combination of trastu

125 to devise a method to establish endothelial cell cultures from human peripheral blood, with an ultim

126 We have established cell cultures from two individual ACC PDX tumors that ma

127 Here, we used primary pituitary cell-cultures from two normal nonhuman-primate species [

128 ession of selected inflammatory mediators in cell culture gain-of-function assays.

129 In cell culture, glutamine deprivation or inhibition of glu

130 Our data provide strong evidence that in cell culture GPI anchor-directed membrane association of

131 The three Cys mutants have minor defects in cell culture growth but a high incidence of abnormal par

132 Standard cell culture guidelines often use media supplemented wit

133 The lack of cell culture has hindered studies of OvHV-2 biology, inc

134 ystem but not in an authentic infectious HCV cell culture (HCVcc) system.

135 virus, this genome proved to be unstable in cell culture; however, propagation of intact virus was a

136 are resistant to replication in conventional cell culture.IMPORTANCE A major challenge in virology is

137 odels of autoimmunity and Th17-skewing human cell culture in vitro.

138 vitro treatment of breast and ovarian cancer cell cultures in aqueous media by tamoxifen and BAY 11-7

139 It also enables time-lapse studies of entire cell cultures in multiple imaging modalities.

140 onal serum plus LIF medium phenocopy male ES cells cultured in 2i/L.

141 We find that cells cultured in adult bovine serum, which better refle

142 Furthermore, we find that female ES cells cultured in conventional serum plus LIF medium phe

143 beta cells from human T2D donors and in INS1 cells cultured in fatty acids that mimic the diabetic st

144 In glycolytic cancer cells cultured in high glucose, we observed a significan

145 long-term LIF-independent self-renewal of ES cells cultured in media containing fetal bovine serum (F

146 l assemblages of encapsulated marrow stromal cells cultured in microwells, osteogenic differentiation

147 ivation in normal human bronchial epithelial cells cultured in organotypic conditions closely approxi

148 We investigated NO signaling in endothelial cells cultured in physiologic (5%) O2 and stimulated wit

149 eliance on glutamine anaplerosis compared to cells cultured in standard tissue culture conditions.

150 found that PI3K inhibitor (PI3Ki)-resistant cells cultured in the absence of PI3Ki developed a proli

151 ely ten times larger than that of individual cells cultured in typical solution media.

152 a feature not seen in conventional monolayer cell cultures, in which most studies of herpesvirus repl

153 aorta, draining lymph nodes, and bone marrow cell cultures, indicating that IRF5 maintains CD11c(+) m

154 cervate/sebum composite barriers prepared on cell culture inserts was determined.

155 of sex in juvenile mice or primary neuronal cell cultures is largely unknown even though both are wi

156 system (SB) and a Caco-2TC7 human intestinal cell culture, is described in this study.

157 DMSR-1 is activated by FLP-13 peptides in cell culture, is required for SIS in vivo, is expressed

158 ancer) and HFF1 (human foreskin fibroblasts) cell cultures, is less potent than tubercidine itself, b

159 In cell culture, it opposed increased cancer cell invasiven

160 it can be proposed as a valuable source for cell culture, its less proliferative capability emerges

161 f IRF-1 against infection of alphaviruses in cell culture, its role in vivo remains unknown.

162 pression of either chicken or human RPE65 in cell culture leads to the production of meso-zeaxanthin

163 ed physiological properties superior to a 3D cell culture matrix.

164 Because MCV cannot be propagated in cell culture, MC159 was expressed independent of infecti

165 type VII collagen was readily detectable in cell culture media and also localized to the dermal-epid

166 cytotoxicity, of toxic species generated in cell culture media by an argon (Ar) plasma jet.

167 rthermore, the amount of recycled albumin in cell culture media corresponded to FcRn-binding affinity

168 itive molecules inside cells and in standard cell culture media generate toxic by-products that inter

169 implemented for monitoring variations in CHO cell culture media upon exposure to high temperature sho

170 (i) generation of stable ENM suspensions in cell culture media; (ii) colloidal characterization of s

171 Blood serum, a common cell culture medium component, is replete with EVs and m

172 x virus type 1 (HSV-1) in detergents-treated cell culture medium containing various serum concentrati

173 his suggests that exposure of virions to the cell culture medium is obligatory during spread in epith

174 [(13)C]Itaconate in the cell culture medium leads to elevated intracellular leve

175 Experiments with cell culture medium showed that virus inactivation by de

176 ng mass spectra of various components of the cell culture medium.

177 persensitivity as an exemplar to (i) develop cell culture methods for the detection of drug-specific

178 ion signals from relatively rare cells while cell culture methods may significantly alter cellular ph

179 Improvements in stem cell culture methods, materials and biophysical tools th

180 s using immunohistochemistry and 3D in-vitro cell culture methods.

181 STAT2-, or IRF9-deficient murine mixed glial cell cultures (MGCs).

182 In LPS-stimulated tubular cell cultures, Mif deletion led to enhanced G2/M cell-cy

183 Using transfected cell cultures, minireplicon systems, virus-like particle

184 Here we show that in a cell culture model of colorectal cancer (CRC) progressio

185 Here, we established a cell culture model to characterize the cell-to-cell tran

186 In this study, we first optimized an ARPE-19 cell culture model to mimic PVR by defining cell density

187 cell differentiation in a three-dimensional cell culture model.

188 Cell culture modeling of diseases can benefit from corne

189 hese miRNAs by dampening their expression in cell culture models and HCV-infected human livers.

190 E) factors for surrogate cancer endpoints in cell culture models and tumor induction in mice vary con

191 Three-dimensional cell culture models have either relied on the self-organ

192 For example, in cell culture models of alpha-syn-seeded aggregation, it

193 In nematodes and mammalian cell culture models of diverse lysosomal disorders, wher

194 habditis elegans as well as murine and human cell culture models of lysosomal diseases.

195 In cell culture models the oxidation status of DJ-1 determi

196 can be used to characterize the response of cell culture models to perturbations such as pharmacolog

197 ue to the lack of efficient and reproducible cell culture models to study HHV-6A/B integration.

198 eness of cancer cells both in vivo and in 3D cell culture models, and this requires active transgluta

199 n the other hand, the employment of 3D tumor cell culture models, especially multicellular tumor sphe

200 ese specific inhibitors, which are active in cell culture models, will be exceptionally useful reagen

201 ipogenic and anti-lipogenic effects of Hh in cell culture models.

202 ncreatic cancer and its TAS in primary human cell culture models.

203 herefore not readily apparent in traditional cell culture models.

204 riation frequently alters gene expression in cell-culture models and differentiated tissues.

205 In cerebrocortical cell cultures, neuroprotection by IFNbeta against gp120

206 al cell reprogramming, we generated a stable cell culture of an extremely rare and aggressive neuroen

207 In this study, we established organotypic cell cultures of AT explants to study the impact of cyto

208 Here we show in cell cultures of the stony coral Stylophora pistillata t

209 known about the effect of antibiotic use in cell culture on gene expression and the extent to which

210 We consider the impacts of cell culture on herpesvirus genomes and how to accuratel

211 Dependence of cell cultures on "whole serum" must be examined carefull

212 y predicting the lineage progression of stem cells cultured on biomaterial substrates with graded nan

213 Metastatic cells cultured on osteo-mimetic surfaces coated with ten

214 ngestion of OS by human fetal RPE and ARPE19 cells cultured on Transwell inserts.

215 NS cells, cultured on decellularized renal scaffolds with b

216 here flow splitting is not required, such as cell culture or droplet generators.

217 unity, as our tests were performed either in cell culture or in immunocompromised animals.

218 displayed on the surface of eHAV produced in cell culture or present in sera from humans with acute h

219 s titers were not significantly increased in cell cultures or in mouse lungs, and the disease was par

220 otherapies in three-dimensional colon cancer cell cultures, or spheroids.

221 In primary PA cell cultures, oxamate also effectively suppressed invas

222 of Maillard products in media did not affect cell culture performance with similar growth and viabili

223 mproves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral

224 hest cell activity compared to the reference cell culture plate and the highest power output was ITO.

225 ted in the basolateral compartment (BC) of a cell culture plate.

226 bility, and the nonspecific binding with the cell culture plates, the extracellular matrices, and the

227 t industrial scale with a standard mammalian cell culture platform and a routine purification protoco

228 A defined, scalable, and resource-efficient cell culture platform can thus rapidly generate high qua

229 ectrophoresis and stable isotope labeling in cell culture proteomics that the TbSTT3A and TbSTT3B pro

230 Human cell culture provides an alternative, but many functiona

231 cability to the fabrication of scaffolds for cell culture, reconfigurable microfluidic channels, and

232 lmer and Sorger present data suggesting that cell culture results indicative of synergistic anticance

233 3D hepatic microtissues, unlike 2D cell cultures, retain many of the in-vivo-like functiona

234 In addition, IL-7-stimulated pro-B cell cultures revealed a reduced differentiation from la

235 array with a high density three-dimensional cell culture served as a functional magnetic resonance i

236 Stable isotope labelling of amino acids in cell culture (SILAC) is a quantitative proteomic method

237 stable isotope labeling using amino acids in cell culture (SILAC), a method that facilitates the deta

238 stable isotope labeling with amino acids in cell culture (SILAC)-based quantitative MS, to capture a

239 h stable isotopic labeling of amino acids in cell culture (SILAC).

240 In primary haematopoietic cell cultures, silencing of TRIB3 facilitated megakaryoc

241 re cultured and grown into three-dimensional cell culture spheroids.

242 ificantly ameliorated cardiac hypertrophy in cell culture studies and in animal models.

243 For some ASD patients, fibroblast cell culture studies have eliminated protein and mRNA sy

244 et 275 days, penetrance 82% at 1 year), with cell culture studies indicating that this difference may

245 Cell culture studies suggest that endothelial EphA2 cont

246 In cell culture studies, the miR-352 inhibitor increased en

247 ulation of cells for more than 5 d through a cell-culture substrate, incorporating hollow 150-nm-diam

248 eased the amount of dissociated gp120 in the cell culture supernatant.

249 ve proteomics analysis of eHAV purified from cell-culture supernatant fluids by isopycnic ultracentri

250 th Japanese fulminant hepatitis-1 (JFH1) HCV cell culture system (HCVcc).

251 d, including the development of an efficient cell culture system and animal models for HBV investigat

252 We developed a cell culture system and characterized HEV particles; we

253 We developed an efficient cell culture system and isolated HEV particles that were

254 ocedure we outline here is applicable to any cell culture system and requires approximately 1 week to

255 Together, these findings identify a cell culture system for functionally exploring the two X

256 treatment or vaccine for the disease and no cell culture system to propagate the virus.

257 In our in vitro T cell culture system, MART1-specific CD8 T cells were exp

258 Using a multiplexed cell culture system, we find that switching between cert

259 daptation of our in vitro primary epithelial cell culture system, we found that prostaglandin E2 (PGE

260 In a cell culture system, we found TNF to have antiviral effe

261 vided by the cleavage event, at least in the cell culture system.

262 nges and possible solutions offered by novel cell culture systems and genome engineering approaches.

263 monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside b

264 g gene-inactivating studies in zebrafish and cell culture systems in vitro, we show that Par3 to be e

265 The development of cell culture systems that provide accurate and physiolog

266 s protocol can be easily applied to existing cell culture systems to study the complete HBV life cycl

267 This study compares several adipogenic cell culture systems under a variety of conditions to as

268 d the numerous studies on mechanics using 2D cell culture systems, the spatial and temporal variation

269 Finally, using computer simulation and cell culture systems, we provide evidence for a role of

270 ne produces dose-dependent SOD1 reduction in cell culture systems.

271 ect in SMA model mice and human motor neuron cell culture systems.

272 ance, and most studies have been confined to cell culture systems.

273 , our finding contradicted observations from cell culture systems.

274 s to complete, and it requires experience in cell culture techniques.

275 However, with current cell culture technologies and bioprocessing, the cost fo

276 In cell culture, the major, but not the minor, variant incr

277 lar communication by membrane nanotubes from cell culture to the whole animal.

278 llowed the engineering of tobacco plants and cell cultures to produce a palette of unique colors.

279 tructs, along with detailed instructions for cell culture, transfection, fixation, heavy-metal staini

280 EVs derived from brain endothelial cell cultures treated with IL-1beta also activated an AP

281 atory protein phospholamban was increased in cells cultured under 5% O2.

282 Cell cultures used in biomedical experiments come in the

283 Gastrin was measured in blood, tissue, and cell cultures using an ELISA.

284 Stable isotope labeling by amino acids in cell culture, using primary cortical astrocytes, indicat

285 ll-free areas, thus providing information on cell culture viability, cellular mechanisms and multicel

286 present study, we describe two quantitative cell culture viral integration systems.

287 course of the Na-/K-ATPase inhibition in the cell culture was demonstrated by the corresponding alter

288 ition, we observed that prion replication in cell culture was inversely related to the levels of expr

289 , hRETNTg(+)Tlr4(-/-) mice, and human immune cell culture, we demonstrate that hRetn binds the LPS re

290 of biochemical reconstitution and mammalian cell culture, we elucidate a mechanism by which the coin

291 ons in kidney with reconstitution studies in cell culture, we found that WNK bodies are dynamic membr

292 Synchronized tobacco BY-2 cell cultures were used to study the role of topoisomera

293 virtually any DNA sequence, particularly in cell culture where selection can be used to recover rela

294 The ability to propagate VA1 in cell culture will facilitate studies of the neurotropism

295 titution (T124N/V165I/T174I) that emerges in cell culture with a representative MINI, KF116.

296 Our data demonstrate that combining 3D cell culture with bioengineering can increase reproducib

297 and 17b are the most potent in human cancer cell cultures with MGM GI50 values of 0.063 and 0.033 mu

298 romising alternative to animals and standard cell cultures with regard to mechanistic insights and pr

299 Our data showed that germ cells cultured with 5H-purin-6-amine could maintain thei

300 GC-sensitive cells cultured with IL-4 display an increased resistance