ライフサイエンスコーパス: cell extract

1 iological samples (e.g., human biofluids and cell extracts).

2 the ribosomal/membrane fraction of bacterial cell extract.

3 vity originating from 380 +/- 20 cancer 293T cell extract.

4 protein substrates in the context of a whole cell extract.

5 SC-related assembly complexes found in human cell extract.

6 ent purification tags directly from HEK-293T cell extract.

7 o isolate the target protein directly from a cell extract.

8 during repair of 8-oxoG in a wild-type mouse cell extract.

9 T content of complexes assembled in cells or cell extract.

10 f phosphopeptides from serum digest and HeLa cell extract.

11 nonfat milk, egg yolk, human serum and HeLa cell extract.

12 has been further tested by analyzing a whole cell extract.

13 ition of viral replication in yeast and in a cell extract.

14 tro and in immunocomplexes purified from the cell extracts.

15 nt clearance of the ErbB-2/HER2 protein from cell extracts.

16 H range 6.1-8.6 with resting cells and crude cell extracts.

17 unoprecipitates with gamma-tubulin Gtb1 from cell extracts.

18 cies and were found to be highly abundant in cell extracts.

19 in pure enzyme fractions as well as in crude cell extracts.

20 method for measuring telomerase activity in cell extracts.

21 eins encoded by RASopathy genes in mammalian cell extracts.

22 s for normalization of metabolomic data from cell extracts.

23 amount of heme-bound mPGES2 was detected in cell extracts.

24 nute protein amounts readily from tissue and cell extracts.

25 also active against endogenous Tdp1 in whole cell extracts.

26 directly against the purified proteins or in cell extracts.

27 fed a diet supplemented with Wigglesworthia cell extracts.

28 e action of DUBs and the proteasome in crude cell extracts.

29 aricus exists as a heterotrimeric complex in cell extracts.

30 led based on studies using Xenopus and human cell extracts.

31 substrate delays nucleosome loading in human cell extracts.

32 as specific targets of these probes in whole cell extracts.

33 purified RFC and in associating with RFC in cell extracts.

34 ied in aqueous phosphate buffer and in CEM/0 cell extracts.

35 with Rab38 and Rab32 from MNT-1 melanocytic cell extracts.

36 activities were identified from M. smegmatis cell extracts.

37 noprecipitation of (35) S-methionine-labeled cell extracts.

38 ils of how to perform a basic assay of whole-cell extracts.

39 at levels similar to those of PrtP in whole-cell extracts.

40 ified phospholipid species from microfluidic cell extracts.

41 ing activity of Shelterin complexes in human cell extracts.

42 nt to induce WAVE-dependent bead motility in cell extracts.

43 of single spliceosomes in real time in whole-cell extracts.

44 and with purified phosphoproteins from whole cell extracts.

45 from impure solutions or even directly from cell extracts.

46 nalyzing lipid-protein interactions in crude cell extracts.

47 nscription (RT) PCR using RSV-infected HEp-2 cell extracts.

48 re slowly in dwa1 and dwa2 than in wild-type cell extracts.

49 mbination with fluorescent derivatization of cell extracts.

50 biquitinated proteins despite high levels in cell extracts.

51 ctivity was assayable in R. prowazekii lysed-cell extracts.

52 on of the illicit drug fentanyl in red blood cell extracts.

53 screening as activators of caspase-3 in HeLa cell extracts.

54 cting chemicals including other biofluids or cell extracts.

55 ing purified components as well as mammalian cell extracts.

56 ied chromatin-bound RECQ4 complex from human cell extracts.

57 methodology to measure G6P concentrations in cell extracts.

58 R is similar to data from PAR synthesized by cell extracts.

59 (Tf-TfR) complexes from insect and mammalian cell extracts.

60 y platinum atomic absorption spectroscopy of cell extracts.

61 d with trypsin or chymotrypsinogen in Paneth cell extracts.

62 enopus laevis egg extracts and mitotic human cell extracts.

63 ry roles in TBSV replication in cells and in cell extracts.

64 nd a decrease in 8-oxoG cleavage activity in cell extracts.

65 upercoils in ICL-containing plasmids in HeLa cell extracts.

66 5F12) to human XPA or in XPC(-/-) fibroblast cell extracts.

67 n of NSP ubiquitylation in Neurospora crassa cell extracts.

68 shared between those formed in cells and in cell extracts.

69 highly selective delivery of the NDP in CEM cell extracts.

70 and Mll4 complexes in size-fractionated beta-cell extracts.

71 tidase activity in proteasomes purified from cell extracts.

72 ins from Thermococcus species 9 degrees N or cell extracts.

73 tners by immunoprecipitation of cross-linked cell extracts.

74 rates of nucleotide excision repair in human cell extracts.

75 NA replication in yeast or in vitro based on cell extracts.

76 in glycoprotein ligand-1) from hematopoietic cell extracts.

77 shown to exist in a common complex in human cell extracts.

78 g partner of full-length CAPERalpha in human cell extracts.

79 ing of R-loops by HeLa and human neuron-like cell extracts.

80 ogram and with labeled standards spiked into cell extracts.

81 glucomutase 1 enzyme activity was assayed on cell extracts.

82 measuring polymer concentration in cells and cell extracts.

83 chemical reactivity and metabolism in liver cell extracts, 15 candidate metabolites were identified

84 The MIC values of the probiotic methanol cell extracts against Candida albicans ranged between 1.

85 II, still bound to native DNA, from HEK-293T cell extract, allowing us to calculate a 25-A-resolution

86 nhibition of cleavage and polyadenylation in cell extract and a decrease in the levels of several key

87 1/dyskerin/NOP10/NHP2 scaffold purified from cell extract and demonstrate that distributed sequence f

88 inding assays were performed using the whole-cell extract and oligonucleotide probes consisting of th

89 Remarkably, cell extract and purified protein experiments show that

90 itro replicase assembly assay based on yeast cell extract and purified recombinant tombusvirus replic

91 the phosphatase activity from a B. subtilis cell extract and suppose that dephosphorylation is catal

92 UreD found to be soluble in Escherichia coli cell extracts and able to complement a DeltaureD-urease

93 )-NTA)(2)-Cy3 to be used as a probe in crude cell extracts and as a His(6)-specific gel stain.

94 Our data suggested that HeLa cell extracts and cultured host cells, but not RRL, cont

95 containing single nucleotide bulges in human cell extracts and discovered that several deaminated or

96 Using HeLa cell extracts and DNA affinity chromatography, we demons

97 nd stoichiometries in protein complexes from cell extracts and from in vitro synthesized components.

98 ay and LC-MS, methylofuran was identified in cell extracts and further purified.

99 y to nitrocellulose-blotted human epithelial cell extracts and IgE levels to environmental allergens

100 s domain inhibit Wee1 degradation in somatic cell extracts and in cells without affecting the overall

101 ence of deoxyadenosine cleavage in T. brucei cell extracts and increased deoxyadenosine sensitivity i

102 are exclusively (homo- or hetero-) dimers in cell extracts and intact cells.

103 p53, co-immunoprecipitates 133p53alpha from cell extracts and interacts only with p53 molecules that

104 and oxidized disulfide glutathione (GSSG) in cell extracts and isolated mitochondria as a measure of

105 rected probes for profiling kinases in whole cell extracts and live cells.

106 Starting with the cell extracts and passivated slides, the assay requires

107 ctional assay that utilizes active mammalian cell extracts and protein microarrays and identified 1,5

108 In cell extracts and purified form, Endo III is generally m

109 Analysis of soluble cell extracts and purified MCPs by Western blotting show

110 A detailed investigation of cell extracts and purified proteins by top-down MS, NMR

111 fluoro-2'-deoxyuridine was observed, both in cell extracts and spent culture medium (i.e. tumor cell-

112 6) complexes also can be purified from human cell extracts and that Orc6 and Orc1 each contain a sing

113 Although BC is found as a dimer in cell extracts and the carboxylase activities of the two

114 ive D478A FAM20C mutant was detected in both cell extracts and the media.

115 5-P form of RNAPII accumulates in both whole-cell extracts and throughout coding regions; additionall

116 binding protein 1 (TopBP1) form complexes in cell extracts and when expressed from baculovirus vector

117 roteins, can be quantitatively depleted from cell extracts, and allows the integration of biochemical

118 selectively enrich GFP fusion proteins from cell extracts, and as affinity column ligands for inexpe

119 ibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over t

120 d into proteins, we show that in live cells, cell extracts, and in vitro reconstituted complexes, Hex

121 ons on recombinant POP and FAPalpha enzymes, cell extracts, and living cells demonstrated high potenc

122 in complex biological samples, such as human cell extracts, and may consequently find future use in f

123 Fascin and Daam1 coimmunoprecipitated from cell extracts, and silencing of fascin led to a striking

124 , urea-polyacrylamide gel electrophoresis of cell extracts, and staining of cells with 4,6-diamidino-

125 or (AR) and AR-coactivator proteins in LNCaP cell extracts, and TET2 KD increases prostate-specific a

126 rate synthase protein and enzyme activity in cell extracts, and the major citrate synthase (citZ) tra

127 using standard protein mixtures and complex cell extracts, and the method was applied to study the p

128 the NEIL1 immunocomplex isolated from human cell extracts, and the two proteins colocalize in the nu

129 ecifically bind tubulin from clarified crude cell extracts, and, after washing, highly purified tubul

130 , Sirt2, and Sirt3 activities from mammalian cell extracts; and determination of IC(50) values of var

131 However, the mechanisms by which B cells extract antigen remain unclear.

132 fore internalization, germinal center (GC) B cells extracted antigen by a distinct pathway using smal

133 Consequently, GC B cells extracted antigen with better affinity discriminat

134 When cell extracts are applied, the surface-tethered antibody

135 y, we show that in the absence of nucleolin, cell extracts are unable to process miR-15a/16 in vitro

136 the effects of polyethylene glycol and yeast cell extract as crowding agents.

137 od eliminates the need to prepare individual cell extracts as samples.

138 CB1R C-terminus could bind to both CRIP1a in cell extracts as well as purified recombinant CRIP1a.

139 to thiophosphorylate its substrates in yeast cell extracts as well as when produced as recombinant pr

140 ndent mRNA translation in a rabbit reticular cell extract assay (RRL-IVT).

141 in cell culture and in a cell-free in vitro cell extract assay, and (iii) LAT can protect C1300 and

142 enzyme and comparable activity in HeLa whole cell extract assays and potentiate the cytotoxicity of t

143 In summary, in human cell extracts, BER and NER activities co-exist and excis

144 In cell extracts, binding of AUF1p45 to TTP potentiated TTP

145 EB1, demonstrated to be present in the whole-cell extracts, binds to both the proximal and distal E2-

146 ed during activation of PrtP) was present in cell extracts but was detected neither in the purified a

147 , and mPGES-2 were readily detected in whole cell extracts but were not regulated by IL-1beta.

148 both in vitro and in neuroblastoma (SH-SY5Y) cell extract by affecting the base excision and AP lyase

149 h the HC-HA complex from agarose-overlaid AM cell extracts by an anti-human IalphaI antibody.

150 g degradation into epitopes in primary human cell extracts by mass spectrometry and on epitope presen

151 mmunoprecipitation of CHD1 and Mediator from cell extracts can be ablated by shRNA knockdown of a spe

152 iation of the two endogenous proteins in the cell extract chromatin fraction is considerably increase

153 e study offers a simple representation of a "cell extract" comprising, e.g., a droplet of actomyosin

154 We now show that HeLa cell extracts contain a factor that promotes ATP- and p3

155 tion of <1 muU (pmol/min) of PDE activity in cell extracts containing 0.25-10 mug protein.

156 ng glycosidase inhibitors by FluoPol-ABPP on cell extracts containing recombinant, overexpressed glyc

157 ion events in complex environments including cell extracts contribute to our understanding of how the

158 HEK293T cell extracts, deficient in hMLH1, are unable to process

159 Mass spectrometric analyses of whole-cell extracts demonstrated that two proteins encoded by

160 Single molecule imaging of motility in cell extracts demonstrates that the CAP-Gly domain of p1

161 ytometry, and fluorescence quantification of cell-extracted dendrimer-Cy5.

162 In addition, we show that the cell extracts derived from MacroD-deficient Neurospora c

163 plying two optimized LC methods to bacterial cell extracts detected more than 200 metabolites with le

164 EMSAs with splenic B cell extracts directly demonstrated that YY-1 binds to t

165 a and topoIIbeta on decatenatory activity in cell extracts, DNA damage and decatenation G(2) checkpoi

166 ic endonuclease-1 (APE1) and APE1 from human cell extracts efficiently process an rAP site in DNA and

167 lving mass spectrometry-based measurement of cell extracts, enable routine quantitation of most centr

168 el sirtuin deacetylation substrates in whole cell extracts, enabling large-scale screens for new deac

169 The proteomic analysis of the cell extract, Endoplasmic Reticulum/Golgi apparatus and

170 nsor of Abl tyrosine kinase activity in HeLa cell extracts, exhibiting low luminescence with extracts

171 We further validated the assay using whole cell extracts, extending its potential application to de

172 separation process of DNA or protein from a cell extract extremely important.

173 The MCM7 or SF3B3 depleted cell extract failed to splice reporter RNA in in vitro R

174 PX2 and demonstrate the utility of mammalian cell extracts for biophysical assays.

175 Fas signaling, we screened primary lymphoma cell extracts for Fas-associated proteins that would hav

176 easured and compared in identical human HeLa cell extracts for the first time under identical conditi

177 ever, been hampered by its rapid reversal in cell extracts, for example through the action of de-ubiq

178 Here, using cell extracts from APTX-deficient cell lines, human Atax

179 ortantly, the activity of TACE was higher in cell extracts from CERK(-/-) as compared with wild type.

180 m both enzymatic assays and Western blots of cell extracts from clostridial transformants harboring p

181 We found that protein-free cell extracts from H. salinarum provided a high level of

182 Using cell extracts from HeLa cells, as well as transfection o

183 different mutations appears similar in whole-cell extracts from lymphocytes, and suggest that measure

184 with the KSHV K-bZIP protein in vitro and in cell extracts from lytically reactivated infected cells.

185 d proteins was analyzed by immunoblotting of cell extracts from P gingivalis and 10 other oral bacter

186 Incubation of the L-selectin tail with cell extracts from phorbol 12-myristate 13-acetate-stimu

187 ern of peripheral chromatin in the nuclei of cells extracted from liver and thyroid specimens is asso

188 em cells is present in CD146(+) perivascular cells extracted from the nonhematopoietic adipose tissue

189 eatures (descriptors) often used to quantify cells extracted from these images have long been shown u

190 Bone marrow and splenic cells extracted from tumor-bearing and control mice (n=

191 of >98% in pre-purified mesenchymal stromal cells, extracted from human dental pulp, with no adverse

192 ition, we observed identical productivity of cell extracts generated from culture volumes spanning th

193 , or in vitro methylation assays using whole cell extracts grown under different conditions.

194 ressed protein translation in cell-free HeLa cell extract; however, it did not affect translation in

195 Mass spectrometric analysis of cell extracts identified 352 proteins that could be matc

196 of coimmunoprecipitated NS5A complexes from cell extracts identified heat shock proteins (HSPs) 40 a

197 bits transcription of 5S rRNA genes in whole cell extracts, if this precedes the stage of initiation

198 ns: immunoblotting, immunoprecipitation from cell extracts, immunofluorescence detection in fixed cel

199 s-linked duplexes were unhooked in mammalian cell extracts in a manner independent of the NER pathway

200 support replication of plasmid DNA in yeast cell extracts in a reaction that exhibits hallmarks of c

201 specific Box D complex formed with infected cell extracts in electrophoretic mobility shift assay ex

202 eat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; howeve

203 litates PARP-1 association with XPA in whole cell extracts, in isolated chromatin complexes, and in v

204 ion of the hydrophilic fraction of the whole-cell extracts increased the antifungal activity of the h

205 Assays of DNA repair in intact cells or in cell extracts indicate that this protein damage compromi

206 Co-immunoprecipitation of the protein from cell extracts indicated that RNase II interacts with its

207 more, the transplantation of iMuSCs or their cell extracts into the muscles of mdx mice (i.e., a mous

208 e primary siRNA-binding protein in the whole cell extracts is Dicer.

209 showed that mRNA 3' end cleavage reaction in cell extracts is strongly but transiently inhibited unde

210 e found a defect in both activities in human cell extracts lacking LIG4.

211 A end joining, and addition of wt-Metnase to cell extracts lacking Metnase markedly stimulated DNA en

212 Cell extracts lacking Metnase poorly supported DNA end j

213 Examination of cell extracts led us to detect a previously unknown xant

214 performed by polymerase chain reaction or by cell extract-mediated recombination.

215 g was quantified in culture supernatants and cell extracts of HuH-7 cells transiently transfected wit

216 rtate was the only source of beta-alanine in cell extracts of M. jannaschii.

217 y overcome by methylating DNA in vitro using cell extracts of M. xanthus prior to transformation.

218 determination of human active vitamin B12 in cell extracts of Propionibacterium freudenreichii subsp.

219 or acrylate to propionyl-CoA was detected in cell extracts of R. sphaeroides grown with 3-hydroxyprop

220 Compared with cell extracts of radiation-sensitive bacteria, H. salina

221 In cell extracts of S. pomeroyi DSS-3, the apparent K(m) fo

222 tric analyses and activity assays with whole cell extracts of strain CBDB1 gave strong evidence that

223 In cell extracts of the M. maripaludis wild-type strain, th

224 thylxanthines was also detected in the crude cell extracts of theophylline-grown CBB5.

225 The NER efficiencies in human HeLa cell extracts of these lesions are significantly differe

226 his issue, we have studied NER in human HeLa cell extracts of two topologically distinct lesions, one

227 compatible and was proven to be inert in in-cell extracts of Xenopus laevis oocytes at 18 degrees C

228 eptional characterization T4 PNK activity in cell extracts, offering a powerful tool for biomedical r

229 us molecules that could be detected in whole-cell extracts, OMV preparations, and lipopolysaccharide

230 plasmid-based assay and repair assays using cell extracts or purified proteins, we have shown that D

231 model was further validated using N. tabacum cell extracts or recombinant N. tabacum protein prenyltr

232 umber of samples containing purified enzyme, cell extracts, or tissue homogenates.

233 neuronal and human embryonic kidney (HEK293) cell extracts overexpressing PAG/Cbp, we show that EphB2

234 NEIL2 immunocomplexes from cell extracts preferentially repaired the mutagenic cyto

235 ddition of purified recombinant GAPDH to the cell extract prepared from GAPDH-depleted yeast results

236 ith a high-molecular weight complex in whole cell extracts prepared from two different cell lines.

237 obtained between 2- and 12-fold dilutions of cell extracts (R(2) = 0.999).

238 AD9 knocked-down human prostate cancer whole cell extracts, relative to controls.

239 The analysis of cell extracts revealed that the heme of GlbN was covalen

240 In cells and cell extracts, RUTBC1 influenced the ability of Rab32 to

241 We used a novel whole-cell-extract screening approach to identify Spt7, a memb

242 of purified PARP-1, and in experiments with cell extracts, self-PARylation was greater in Hmgn1(+/+)

243 inant cytoplasmic exonuclease Xrn1 and liver cell extracts show that miR-122-mediated protection of t

244 x, with immunoprecipitation experiments from cell extracts showing association of hsc70 with wild typ

245 The double mutant D149C/S193C purified from cell extracts shows decreased sensitivity to inducer bin

246 sensor to quantify CDK4 activity in melanoma cell extracts, skin biopsies and melanoma xenografts.

247 Moreover, adding ubiquitinated proteins to cell extracts stimulated proteasome binding of both enzy

248 bonucleoprotein-free state of tRNA halves in cell extract suggest that ciliate tRNA halves are degrad

249 t, restores ALY recognition in IPMK-depleted cell extracts, suggesting a mechanism underlying transcr

250 oU proteins were partially activated by HeLa cell extracts, suggesting that a host cell cofactor othe

251 trates from a simple assay with concentrated cell extracts suggests that combining microarray analysi

252 ying RNA-dependent PABP-eIF4G association in cell extracts suggests that RNA1, the PABP-binding domai

253 eadily copurified from Y. pseudotuberculosis cell extracts, suggests that these two RNases regulate T

254 d Sit4 physically interact with eIF2alpha in cell extracts, supporting their direct roles as eIF2alph

255 Crude cell extracts synthesize ITA via decarboxylation of cis-

256 ght into somatic cells, we developed a human cell extract system that recapitulates CDK1 activation a

257 Because cell extract systems only approximate in vivo conditions

258 ubiquitinating (DUB) activity exists in HeLa cell extracts that is highly specific for cleaving K63-l

259 We show by using cell extracts that Ku is essential for the efficient rem

260 this reaction has been detected in mammalian cell extracts, the protein(s) responsible remain unknown

261 ous activity of both alpha-galactosidases in cell extracts, thereby providing a means to study the pr

262 tating active RP534 from R. prowazekii lysed cell extracts, thus verifying that this protein is expre

263 ced in a PDZ-regulated manner by addition of cell extract to near in vivo levels.

264 ct) that utilizes easy to generate bacterial cell extracts to assemble multiple DNA fragments into re

265 IA-ESI-MS).We used series dilutions of HL-60 cell extracts to establish the relationship between cell

266 eins, Kel1 and Kel2, associate with Bud14 in cell extracts to form a stable 520-kDa complex with an a

267 used to purify these proteins from bacterial cell extracts to high purity in a single step.

268 ere we used affinity chromatography of tumor cell extracts to identify LGALS3BP as a novel sialic aci

269 tion and reduces the capacity of Arabidopsis cell extracts to ligate a nicked DNA intermediate.

270 id adenine dinucleotide phosphate (NAADP) in cell extracts using surface-enhanced Raman spectroscopy

271 RNAs spliced from biotin-tagged pre-mRNAs in cell extracts, using antibodies to EJC components Y14 an

272 Spike recoveries in cell extracts vary from 53% to 127% averaging 91%.

273 ation has been extensively investigated with cell extracts, viral DNA replication in productively inf

274 al complement-evasion molecules, C. albicans cell extract was absorbed to a factor H-coupled matrix.

275 n vitro assembly of the viral replicase in a cell extract was inhibited by the cytosolic fraction obt

276 ion in the endogenous pol beta gene, and the cell extract was subjected to an 'affinity-capture' proc

277 ith the fabAB promoter detected in wild-type cell extracts was absent in the desT deletion strain, as

278 ion experiments (IPs) carried out with whole-cell extracts (WCEs) revealed that hemagglutinin (HA)-ta

279 By fractionating human cell extract, we purified the E3 ubiquitin ligase Mule (

280 sing affinity purifications from C. albicans cell extracts, we demonstrate that CaTAF12L uniquely ass

281 sembly on membrane-coated beads in mammalian cell extracts, we found that Rac1 was not sufficient to

282 environmentally regulated FLO11 promoter in cell extracts, we identified 15 regulators that bound sp

283 In cell extracts, we identified a novel activity that speci

284 lectrophoretic mobility shift assays in HeLa cell extracts, we show that OA treatment disrupts pre-mi

285 o note that the measured quantities from the cell extract were found to constitute a statistically si

286 Cell extracts were analyzed for mutant protein stability

287 rmal NER activity appeared when the XPC(-/-) cell extracts were complemented with XPC-RAD23B proteins

288 treated with 100 microg/ml IgG for 6 h, and cell extracts were examined by immunoblot using Abs to p

289 When MV-4-11 cell extracts were immunodepleted of AUF1, the rate of d

290 Whole-cell extracts were obtained from cultured human corneal

291 the role of Cernunnos in end joining, whole-cell extracts were prepared from Cernunnos-deficient hum

292 with both polysomal and monosomal RNA in S2 cell extracts, whereas Y14 and MAGO fractionate separate

293 ision repair (NER) experiments in human HeLa cell extracts which show that the G*CT* intrastrand cros

294 R was deficient in the mms19 deletion mutant cell extracts, which was complemented by the NER/transcr

295 ited PCNA-dependent DNA synthesis in a human cell extract with improved avidity when compared with th

296 found that Def1 copurifies from yeast whole-cell extract with TFIIH, the largest general transcripti

297 Immunoprecipitation of muscle cell extracts with a PUM2 specific antibody precipitated

298 yeast cells, as primase does not interact in cell extracts with pol1 that either terminates at residu

299 agged protein can be specifically labeled in cell extracts without protein purification and then can

300 plexes with single Sp or Gh lesions in human cell extracts yields a characteristic nucleotide excisio