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1 hieved for the detection of ERalpha in MCF-7 cell lysate.
2 of the kinase activity and inhibition in the cell lysate.
3 ntingtin aggregation in a cell model and its cell lysate.
4 e complex and slow with both whole cells and cell lysate.
5 ous concentrations in prostate cancer (PC-3) cell lysate.
6 complex biological samples such as serum and cell lysate.
7 the extraction of plasmid DNA from bacterial cell lysate.
8 ve standard deviations of up to 70% in crude cell lysate.
9 these ligands, even in samples as complex as cell lysate.
10 ies of pyrophosphopeptide in the presence of cell lysate.
11 ormation from nanogram quantities of protein cell lysate.
12 with standard protein mixtures and a complex cell lysate.
13 ross-linked protein-DNA complexes from yeast cell lysate.
14 with a concomitant retention of sGAG in the cell lysate.
15 is advantageous to measure miRNA in a crude cell lysate.
16 ide gel electrophoresis (PAGE) of the single-cell lysate.
17 s was performed in both a pure buffer and in cell lysate.
18 d analysis of picogram loadings of RAW 264.7 cell lysate.
19 nd dimers were successfully pulled down from cell lysate.
20 cterize full-length otoferlin from mammalian cell lysate.
21 espite the presence of growth media and Vero cell lysate.
22 h the protein(s) they react with in cells or cell lysate.
23 ed MALAT1 ENE+A RNA upon addition of HEK293T cell lysate.
24 mutant virus protein in the transfected BHK cell lysate.
25 strated with standard proteins and a complex cell lysate.
26 rks for complex mixtures of trypsin-digested cell lysate.
27 om a complex sample such as blood, serum, or cell lysate.
28 ectrometer for three proteins and an E. coli cell lysate.
29 at high concentrations as well as of a crude cell lysate.
30 es, to complex aggregates, as found in total cell lysates.
31 vity changes in hundreds of cysteines within cell lysates.
32 ipitated with IGF1 receptor (IGFR1) in whole-cell lysates.
33 mains that are unstructured when analyzed in cell lysates.
34 of NAAAR mutant gene libraries directly from cell lysates.
35 w separation of as little as 20 mug of total cell lysates.
36 little as 25 mug tryptic peptides from whole cell lysates.
37 extraction of phosphopeptides from the whole cell lysates.
38 PLC fractionation of Chlamydia-infected HeLa cell lysates.
39 ures and for protein-protein interactions in cell lysates.
40 single-molecule motility assays of mammalian cell lysates.
41 -fructofuranosidase activity in T. vaginalis cell lysates.
42 and can be recapitulated by eosinophil whole-cell lysates.
43 molecules and unpurified protein targets in cell lysates.
44 rove their binding to endogenous PDEdelta in cell lysates.
45 er to enrich for such methylated lysine from cell lysates.
46 ably Escherichia coli cell free extracts and cell lysates.
47 he specificity of an inhibitor of calpain in cell lysates.
48 able to profile molecular activities within cell lysates.
49 the LMM fractions of both tumor and nontumor cell lysates.
50 and inhibits the enzyme's lyase activity in cell lysates.
51 of tools for profiling enzyme activities in cell lysates.
52 ogram or sub-picogram levels per nanogram of cell lysates.
53 ) as an interaction partner of Arl8b from NK cell lysates.
54 g it more versatile than other methods using cell lysates.
55 rring amyloid-forming proteins directly from cell lysates.
56 porin A) in complex protein mixtures such as cell lysates.
57 identifying patterns of enzyme activities in cell lysates.
58 tivities of 3'-5' exonuclease and DNase I in cell lysates.
59 ns such as e.g., biotechnologically relevant cell lysates.
60 chia coli were quantitatively estimated from cell lysates.
61 tivity of ITPA in bacterial, yeast and human cell lysates.
62 of overexpressed proteins directly in crude cell lysates.
63 (CANC) nanotubes captured SUN1 and SUN2 from cell lysates.
64 molecules and unpurified protein targets in cell lysates.
65 relevant concentrations of ERBB2/neu/Her2 in cell lysates.
66 known endogenous protein binders in complex cell lysates.
67 eins added in crude Escherichia coli or 293T cell lysates.
68 tion of SPSB2-iNOS interaction in macrophage cell lysates.
69 7 bacteriophage and His6-GroEL directly from cell lysates.
70 t of hTS with one such peptidic inhibitor in cell lysates.
71 in the lysosomal fraction, but not in whole-cell lysates.
72 e for two different grades of bladder cancer cell lysates.
73 es individual caspases can cleave in complex cell lysates.
74 t of detection 0.3ng/mL (1.5pM) for ERBB2 in cell lysates.
75 ystem for triplicate analysis of a RAW 264.7 cell lysate; 2 +/- 1 and 7 +/- 2 protein groups were con
76 amine 500 mug of a human foreskin fibroblast cell lysate a total of 1,944 unique phosphopeptides from
77 n (out of 35 fractions) from a crude HEK 293 cell lysate, a total of 640 proteins were identified in
79 is capable of detecting miRNA-143 in cancer cell lysates, allowing for the discrimination between th
80 rcial reagent that can easily generate crude cell lysates amenable to direct analysis by one-step RT-
83 is was achieved by coating MPs with human ES cell lysate and centrifugation of specific ratios of ES
84 s to induce coacervation in Escherichia coli cell lysate and follow gene expression under crowded and
86 in vitro translation assay containing human cell lysate and purified target mRNA fused to a reporter
87 SOD, GR, and CAT activities in red blood cell lysate and saliva and MDA levels in plasma and sali
88 single-molecule pull-down (SiMPull) from HEK cell lysate and subunit counting in the plasma membrane
90 generation of protein alpha-thioesters from cell lysates and applied this strategy for the semisynth
91 of this iBody for the isolation of FAP from cell lysates and blood serum as well as for its detectio
92 ere also detected and semiquantified in both cell lysates and cell culture supernatants by Western bl
93 l, we tested varicella zoster virus-infected cell lysates and clinically isolated virus and found evi
94 We also found that HpGroES bound to TLR4 in cell lysates and colocalized with TLR4 on the cell membr
95 sed for quantitative PCR, while protein from cell lysates and conditioned media were used for immunob
98 and in vitro by immunoprecipitation in crude cell lysates and from fractions after gel filtration and
99 nation of the presence of SLT-1 from complex cell lysates and ham/juice samples based on the detectio
103 reduced the rate of c-di-GMP degradation in cell lysates and inhibited the activity of EAL-dependent
104 in CSF-1R-deficient U251 human glioblastoma cell lysates and inhibited the proliferation, clonogenic
106 on by protein tyrosine phosphatases (PTP) in cell lysates and single cells and to investigate the eff
107 as biocatalysts was investigated using crude cell lysates and soluble protein fractions of Azospira o
110 ines demonstrated that LH2 is present in the cell lysates and the conditioned media in a dimeric, act
111 nd coproporphyrin, based on HPLC analysis of cell lysates and the culture medium, as well as cell-fre
112 sed to quantify the extent of interaction in cell lysates and the equilibrium binding constant for th
113 n complex physiological environments such as cell lysates and to measure their individual activities
114 ed that CacyBP/SIP forms a homodimer in NB2a cell lysate, and biophysical methods demonstrated that C
115 sis of immunoprecipitation product from OSCC cell lysate, and in situ proximity ligation assays.
116 t antiserum was used for Western blotting of cell lysates, and gel slices corresponding to reactive b
117 from different proteomic setups (live cells, cell lysates, and immobilized affinity matrix), we found
119 tion of NF-kappaB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragm
120 EF1G were identified in fractionated human T-cell lysates as reverse transcription cofactors, as thei
121 lex, is broad in scope, and is applicable in cell lysates as well as to covalent inhibition/modulatio
124 SPAG1 was present in human airway epithelial cell lysates but was not present in isolated axonemes, a
125 elivery of B. burgdorferi DNA, RNA, or whole-cell lysate, but not by lysate that had been treated wit
126 port on accurate miRNA quantitation in crude cell lysate by a CE-based hybridization assay termed dir
128 ermore, the alkyne-tagged glycoproteins from cell lysates can be directly detected with AzBOCEt in ge
129 , Kd values for unpurified proteins in crude cell lysates can be obtained without prior knowledge of
130 n vitro DISC is assembled in the presence of cell lysate, caspase-8 Y380 phosphorylation attenuates D
131 immunotherapy consisting of autologous tumor cell lysate combined with toll-like receptor ligands.
132 ied a high quantity of mRNA from crude yeast cell lysate compared to a phenol/chloroform extraction m
133 of soluble, assembled RuBisCO recovered from cell lysates compared with co-expression of RuBisCO with
135 that pretreatment of cell free extracts and cell lysates containing Sav mutants with diamide affords
138 Protein captured from a crude bacterial cell lysate could also be deuterated without the need fo
139 ltiple kinase activity profiling from single cell lysate could potentially allow us to study heteroge
140 cellular phenoloxidase activity (present in cell-lysates), demonstrates >98% reduction in activity a
142 or was applied to detect miRNA-215 from A549 cell lysate directly without complex sample treatment, a
145 inked small molecules and a model library of cell lysates expressing SNAP-target fusions combined in
151 ntify changes in global activity profiles of cell lysates for a wide variety of enzymatic activities.
154 hrome c (Cyt c) is an important biomarker in cell lysates for the early stage of apoptosis or antican
156 SY and 2D (13)C-(1)H HSQC-TOCSY spectra of a cell lysate from E. coli, which yields a substantial imp
157 Escherichia coli cell lysate with 100 mug of cell lysate from mouse, corresponding to expected relati
158 Furthermore, in vitro assays with crude cell lysate from PCE grown cells revealed dechlorination
159 is of GLD is based on GALC activity of total cell lysates from blood, which does not discriminate whe
162 l inflammation, ii) the capacity of necrotic cell lysates from HT29 cells or human IECs to induce hum
163 for the purpose of generating RT-qPCR-ready cell lysates from MDCK cells infected with influenza vir
164 ryosections, and the protein was detected in cell lysates from synovium obtained from OA patients.
166 e corresponding antigen from a complex whole-cell lysate generates a change in refractive index in th
167 analysis (HILIC-RPLC) of S. cerevisiae whole cell lysate has been used to acquire retention informati
169 -top-down MS analysis of Pyrococcus furiosus cell lysate identified 134 proteins and 291 proteoforms
171 n isoforms in immune-purified sample and raw cell lysate in 2 hours with sample volume requirements o
172 es; for example, it could be used to analyze cell lysate in drug response studies or pricks of blood
173 -1 protein is 0.0078mg/ml of T24 (Grade III) cell lysate in phosphate buffered saline, artificial uri
174 in our platform by both tracking fluorescent cell lysate in sealed microwells and with a human-mouse
175 report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of
177 ns identified in a tryptic digest of E. coli cell lysate increased by 13% and 14%, respectively, alon
178 e receptor ligands and M. tuberculosis whole-cell lysate, increased M. tuberculosis replication, and
179 rine A with cyclophilin A protein in a yeast cell lysate is successfully detected and quantified usin
183 in each microwell; (iii) PAGE of each single-cell lysate; (iv) exposure of the gel to UV light to blo
184 probes capable of assessing CTB activity in cell lysates, living cells, and animal models of disease
185 rease in CEST image contrast was observed in cell lysates (mean +/- standard deviation, 0.52% +/- 0.0
188 Chemical treatments of M. tuberculosis whole-cell lysates (MtbWL) ruled out protein, nucleic acid, an
190 was used to detect protein hydroperoxides in cell lysates obtained from macrophages exposed to visibl
192 tudy, a microparticulate vaccine using whole cell lysate of a murine ovarian cancer cell line, ID8 wa
195 richment approach was initially applied to a cell lysate of the filamentous fungus Aspergillus niger.
197 Further, the measurement of cyt c release in cell lysates of cardiomyocytes using the GNP/PPy based i
199 ling lysine deacetylase (KDAC) activities in cell lysates of the CHRF megakaryocytic (Mk) cell line.
201 e responsible for degrading pGpG, we assayed cell lysates of WT and orn strains of P. aeruginosa PA14
202 9) developed titers of IgG response to whole-cell lysates of Y. pestis (YpL) and subunit LcrV similar
203 alization of the banding patterns from whole-cell lysates on SDS-PAGE gels, by direct staining and/or
205 ates, have activity in the presence of human cell lysate or BSA, and catalyze dephosphorylation of a
206 However, selectivity was restored by crude cell lysate or purified G-actin, which joined PPP1R15-PP
209 on and transformation results collected from cell lysates or fixed-cells conceal important dynamic in
212 a linear dependence on the concentration of cell lysate present while specificity is demonstrated by
213 d band broadening, even when handling single-cell lysate protein concentrations in an open device.
214 eport here that HBc expressed in a mammalian cell lysate, rabbit reticulocyte lysate (RRL), was able
218 ide protein interactions in E. coli and HeLa cell lysates, respectively, identifying 1,158 and 3,301
220 recipitation of viral proteins from infected cell lysates revealed association of UL84, UL44, and nuc
222 were determined for miRNA samples in a crude cell lysate, RNA extract from the lysate, and a pure buf
224 teins from 9 injections from a single Jurkat cell lysate sample consisting of 400 ng of total protein
228 at 37 degrees C incubation for 1-2 hours, in cell-lysate samples either freshly prepared or previousl
229 omplexes immunoprecipitated from RV-infected cell lysates show both forms of NSP2 are phosphorylated,
232 Moreover, the addition of purified RelE to cell lysates shows promise as a method for generating ri
233 of effectively enriching SLT-1B from complex cell lysates simply by pipetting 20 muL of the sample in
234 l samples was assessed by testing samples of cell lysate solutions obtained from human astrocytoma (g
235 binding domains (LBDs) can be recruited from cell lysates specifically onto their target phospholipid
236 r the extraction of DNA from crude bacterial cell lysate spiked with 1 pg mL(-1) template DNA without
237 din-Sepharose beads to GL-biot-treated DU145 cell lysates, STAT3 was isolated and identified as a tar
238 t predominately precipitates with Hsp70 from cell lysates, suggesting a role for SGTA in early, Hsp70
239 e specific activity of M. tuberculosis whole-cell lysates, suggesting that a polysaccharide was requi
240 t of affinity-modulating adaptor proteins in cell lysates that would be absent in ligand screening or
241 uted by 100-fold in a trypsin-digested whole cell lysate, the O-GlcNAcylated peptide remains detectab
242 on with an immunoassay-based analysis of the cell lysate, the platform allowed the selective and sens
243 ollowing PIL-based SPME of DNA from a dilute cell lysate, the qPCR amplification efficiency was deter
245 onality) to a tryptic digest of whole Jurkat cell lysate to estimate the depth of proteome coverage a
249 cted hydrogen-deuterium exchange of quenched cell lysates to measure individual opening free energies
250 ty purified PI4KIIalpha from isotope-labeled cell lysates to quantitatively identify interactors.
251 ible to purify phytase simply by heating the cell lysate, to drive aggregation of non-cyclized protei
252 g a multistep enrichment strategy from whole cell lysate, to evaluate their abilities to enrich for d
253 ged protein complexes from minute volumes of cell lysates under close to physiological conditions and
255 dividual SOS molecules are captured from raw cell lysate using Ras-functionalized supported membrane
257 ferase activities of purified proteins or in cell lysates using a mass-differentiated carbohydrate li
259 olute quantitation of endogenous proteins in cell lysates using an automated capillary immunoassay sy
260 ) can be specifically pulled from K562 human cell lysates using beads decorated with phosphorylated g
261 s can be assessed by fractionating tissue or cell lysates using differential ultracentrifugation thro
262 nhibition by small molecules and activity in cell lysates using parallel DNA sequencing or quantitati
263 ions (1 x 10(2) CFU/ml) was detected in FCDI cell lysates using real-time PCR with greater consistenc
264 nd quantitation of carbonyl metabolites from cell lysate was accomplished using a carbonyl-reactive f
265 S analysis (SCX-RPLC) of S. cerevisiae whole cell lysate was used to generate a retention dataset of
267 resis to grossly enrich GSK-3beta from whole cell lysate, we discover by MRM-MS a novel O-GlcNAcylate
268 e-adapted sedimentation velocity analysis of cell lysates, we collated a multidimensional view of Htt
269 arget 6 and Mycobacterium tuberculosis whole-cell lysate were associated with reduced bacillary burde
271 or this purpose, tryptic peptides from whole cell lysates were analyzed by sheathless CE-ESI-MS/MS an
276 could also be detected in dimeric form when cell lysates were subjected to SDS-PAGE under non-reduci
277 eriments using retinal or transfected HEK293 cell lysates were used to investigate the association be
278 ge tubes, a suitable method for studies with cell lysates when enzyme activity was moderate; and (iii
279 ed affinity probes report on KAT activity in cell lysates, where KATs exist as multiprotein complexes
282 o the quantitative determination of Cyt c in cell lysates, which opens a new avenue to early diagnost
283 fied a subset of HDAC8 substrates from human cell lysates, which were further validated for catalytic
284 mixing 5, 10, 15 and 20 mug Escherichia coli cell lysate with 100 mug of cell lysate from mouse, corr
286 ferase added in the Escherichia coli or 293T cell lysate with better staining sensitivity than conven
287 and purification of DNA from crude bacterial cell lysate with subsequent quantification by real-time
288 ctivated as verified by cotreatment of HepG2 cell lysates with (2E)-hexadecenal and the acyl-CoA synt
289 thylated proteins are enriched by incubating cell lysates with 3xMBT, or with the binding-null D355N
290 rated using proteome-wide reactions of HT-29 cell lysates with a model probe of threonine beta-lacton
292 mmunoprecipitation assay of mouse ameloblast cell lysates with either ameloblastin or Psma3 antibody
293 hibited dephosphorylation of the reporter in cell lysates with IC50 values of 470 nM, 35 muM, and 100
294 formation can be performed in blood serum or cell lysates with minimal interference from biomolecules
296 large number of MTs (>50) from human cancer cell lysates with remarkable specificity over other clas
297 ns, Western blotting of SHFV-infected, MA104 cell lysates with SHFV nsp1 protein-specific antibodies
298 attomoles of glycoproteins of interest from cell lysates, with sensitivity several orders of magnitu
299 tone inhibited STAT3 binding to DNA in DU145 cell lysates without affecting phosphorylation status of
300 of a low-abundance transcription factor from cell lysates without need for isolation or enrichment.
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