ライフサイエンスコーパス: cell lysis

1 the full-length Lysin A caused M. smegmatis cell lysis.

2 ity is created by rapid ATP depletion during cell lysis.

3 ligands and the subsequent impairment of NK cell lysis.

4 e and impairing CD8(+) T cell-mediated tumor cell lysis.

5 se mixed lineage kinase domain-like to drive cell lysis.

6 ers the lytic cycle of phage propagation and cell lysis.

7 enables its conditional inactivation during cell lysis.

8 cterial surface to evade complement-mediated cell lysis.

9 TF activity was increased dramatically after cell lysis.

10 s on the cell surface prior to virus-induced cell lysis.

11 pore in the host cell membrane, resulting in cell lysis.

12 ular P. gingivalis was quantified after host cell lysis.

13 by serial invasion attempts leading to host cell lysis.

14 thal effect on the sgrS mutant, resulting in cell lysis.

15 ted with an inhibition of CTL-mediated tumor cell lysis.

16 formation and IL-1beta cleavage occur before cell lysis.

17 r level of quantitation, and did not require cell lysis.

18 cells that are particularly refractory to NK cell lysis.

19 surface nanostructure, leading eventually to cell lysis.

20 rolases (PGHs) are responsible for bacterial cell lysis.

21 susceptibility, misplaced division septa and cell lysis.

22 l cell physiology was not perturbed prior to cell lysis.

23 in the osmotic environment, thus preventing cell lysis.

24 lement-dependent antibody-mediated red blood cell lysis.

25 all compartment in the absence of detectable cell lysis.

26 witch and upon filament assembly, leading to cell lysis.

27 of influenza virus, which was accompanied by cell lysis.

28 MD3 lead to a rapid loss of viability and cell lysis.

29 l morphology, inhibiting growth and inducing cell lysis.

30 ns (ATIs) that can protect infectivity after cell lysis.

31 equired for growth with depletion leading to cell lysis.

32 ate (PEP) becomes reduced several days after cell lysis.

33 factors, and in nutrient redistribution, via cell lysis.

34 me, causing membrane damage and, ultimately, cell lysis.

35 5) and helix V) in the G117C mutant prevents cell lysis.

36 disruption of cellular structure, leading to cell lysis.

37 tions resulting in membrane perturbation and cell lysis.

38 rodrug also induced T cell mediated leukemia cell lysis.

39 play a major role in NK cell-mediated tumor cell lysis.

40 hich also contributes to adenovirus-mediated cell lysis.

41 trix metalloproteases or complement-mediated cell lysis.

42 T molecules to be more potent in directing B-cell lysis.

43 ophagosome is sufficient for virally induced cell lysis.

44 autophagy plays a role in adenovirus-induced cell lysis.

45 ng anemia due to protein instability and red cell lysis.

46 ) into culture medium as a reporter of yeast cell lysis.

47 eath caused by autophagy, cell rounding, and cell lysis.

48 ace, which is directly responsible for tumor cell lysis.

49 nt breaches in wall integrity that can cause cell lysis.

50 d then adsorbed to an anti-FLAG matrix after cell lysis.

51 osmolytes, relieving pressure and preventing cell lysis.

52 ged interactions that led directly to target cell lysis.

53 on in HBV-producing hepatocytes with minimal cell lysis.

54 erent serotypes in the absence of detectable cell lysis.

55 l timing, progression and completion of host cell lysis.

56 y, which enhanced their susceptibility to NK-cell lysis.

57 more than an 80% decrease in specific target cell lysis.

58 that can reside in macrophages without host cell lysis.

59 activity, rendering tumors susceptible to NK cell lysis.

60 actors in the process of beta-lactam-induced cell lysis.

61 pproximately 80% of cells undergo obligatory cell lysis.

62 stability and sensitivity of specific target cell lysis.

63 ting membrane permeabilization and red blood cell lysis.

64 lecular events that promote efficient target cell lysis.

65 t genes -S, R, Rz and Rz1- dedicated to host cell lysis.

66 e and to enhance the effectiveness of target cell lysis.

67 ted with early biochemical evidence of tumor cell lysis.

68 blocking CD40 signaling and by ADCC-mediated cell lysis.

69 of both perforin and CD95 eliminates target cell lysis.

70 DV-infected cells and mediate natural killer cell lysis.

71 ate some of this carbon during infection and cell lysis.

72 hysiological conditions or before programmed cell lysis.

73 t copurifies with nucleoids following gentle cell lysis.

74 e as molecular control elements of bacterial cell lysis.

75 on relative to UAMS-1, indicative of reduced cell lysis.

76 om the artifact of cytosine deamination upon cell lysis.

77 prevent C3b deposition and, thus, autoimmune cell lysis.

78 k complex that mediates complement-dependent cell lysis.

79 smotic change in red blood cells, leading to cell lysis.

80 reased extracellular polymeric substance and cell lysis.

81 from the phagosome, which can result in host cell lysis.

82 signal transduction and induce apoptosis and cell lysis.

83 ytosis, and Ab-dependent complement-mediated cell lysis.

84 ing barrel-like pore structures that lead to cell lysis.

85 ion of the lytic activity to prevent overall cell lysis.

86 nto the peptidoglycan that ultimately led to cell lysis.

87 rocesses were critical for successful target-cell lysis.

88 s spectrometric analysis was performed after cell lysis.

89 ically engineered to induce selective cancer cell lysis.

90 intracellular substrates required for target cell lysis.

91 a beta-barrel transmembrane pore, leading to cell lysis.

92 pression of NK cells and induced MDSC target cell lysis.

93 rane lipids enable intracellular delivery or cell lysis.

94 polymerase chain reaction (RT-PCR) following cell lysis.

95 f the cell's plasma membrane area to prevent cell lysis.

96 rocytes that preceded and were not caused by cell lysis.

97 gated proteins is usually thought to require cell lysis.

98 -MRSA demonstrated reduced susceptibility to cell lysis (1.78-fold; P = 0.032) and antimicrobial pept

99 RVA and RVC NSP4s induced BL21-pLysS E. coli cell lysis, a classical viroporin activity assay.

100 s into the growth medium is not due to gross cell lysis, a conclusion that is supported by several li

101 Finally, with an additional step of cell lysis, a liquid chromatography/mass spectrometry-ba

102 ncrease the efficiency of adenovirus-induced cell lysis, a mechanism that has not been clearly descri

103 in cell permeability, and ultimately caused cell lysis accompanied by the production of membrane tub

104 ESAT-6 (MtbESAT-6) reportedly shows membrane/cell-lysis activity, and recently its biological roles i

105 comedonecrosis, the model predicts: necrotic cell lysis acts as a biomechanical stress relief and is

106 equally cytolytic ( approximately 80% target cell lysis after 4 h), consistent with the similar level

107 utathione, followed by growth inhibition and cell lysis after 6 days.

108 -domain factors were delayed in the onset of cell lysis after treatment with ampicillin.

109 Phage induced cell lysis also indirectly contributes to organic and in

110 s of anti-chemokine receptor-3 reduce muscle cell lysis, although lysis of mdx muscle membranes is no

111 induced Hill coefficients of 0.69 for target cell lysis and 0.68 in interferon secretion.

112 e autophagy, which is required for efficient cell lysis and adenoviral spread.

113 tor receptor (EGFR)/Ras pathway, followed by cell lysis and anticancer immunity.

114 mycobacteria was developed by optimizing the cell lysis and assay conditions.

115 of phdA in DeltabfmR restored eDNA release, cell lysis and biofilm formation to wild-type levels, wi

116 Further, cell lysis and biofilm formation were governed by the Sr

117 ons for each stage of the method, comprising cell lysis and bisulfite (BS) conversion, preamplificati

118 lls elicits the effector functions of target cell lysis and cytokine production.

119 I secretion apparatus, eventually leading to cell lysis and death.

120 abfmR mutant biofilms demonstrated increased cell lysis and eDNA release suggesting BfmR to suppress,

121 in (AT), a virulence factor that causes host cell lysis and elicits inflammasome-mediated IL-1beta se

122 okine responses, resulting in enhanced tumor cell lysis and expansion of human tumor antigen-specific

123 ls are thought to be perforin (Prf)-mediated cell lysis and gamma interferon (IFN-gamma)-mediated ind

124 te enrichment techniques including red blood cell lysis and immunomagnetic purification.

125 cell suspension is filtered before red blood cell lysis and incubated with the following antibodies:

126 is, a program of cell death characterized by cell lysis and inflammatory cytokine release.

127 ural Cytotoxicity Receptor 3) induces target cell lysis and is also crucial for the interaction with

128 biting TGF-beta signaling in T cells reduced cell lysis and leukocyte infiltration in corneas and tri

129 Cell lysis and MAC formation were measured by FACS and i

130 overall workflow consists of methanol-based cell lysis and metabolite extraction with ultrasonicatio

131 d hydrodynamic modeling were used to examine cell lysis and molecular delivery produced by picosecond

132 Although cell lysis and outer-membrane vesicle extrusion are poss

133 After cell lysis and phenol:chloroform extraction, the resulti

134 es that hydrolyze peptidoglycan resulting in cell lysis and release of bacteriophages.

135 h under external mechanical vibration caused cell lysis and released DNA in the supernatant.

136 Following cell lysis and sampling crude cell lysate for analysis,

137 more, histone-induced increases in red blood cell lysis and splenic clearance may be a significant fa

138 hiocyanate, respectively, revealed extensive cell lysis and swelling of cells, consistent with an ins

139 involved in biofilm formation by controlling cell lysis and the release of genomic DNA, which ultimat

140 a semipermeable membrane, enabling efficient cell lysis and transcript capture.

141 This approach led to direct tumor cell lysis and triggered innate immune-mediated attack o

142 enomes is prone to artifacts associated with cell lysis and whole-genome amplification.

143 e those that recruit neutrophils, cause host cell lysis, and are involved in the formation of the fib

144 Given that most MC-LR is released only upon cell lysis, and coupled with the moderate strength of th

145 eliminate penicillin- and vancomycin-caused cell lysis, and dramatically increase tolerance to hydro

146 ses to infection, as evidenced by apoptosis, cell lysis, and phagocytosis of infected cells.

147 Necroptotic cell lysis, and resultant release of proinflammatory med

148 nctional AmiA or AmiB but not AmiC to induce cell lysis, and that the loss of NlpD phenocopies an Ami

149 A novel cell lysis approach and a larger binding surface through

150 Effective cell lysis approaches that completely release intracellu

151 susceptible to natural killer (NK)-mediated cell lysis as compared with parental cytarabine-sensitiv

152 in the final outcome (incomplete vs complete cell lysis) as revealed by the dynamic laser light scatt

153 esulted in lemon-shaped bacteria followed by cell lysis, as previously reported for MreB inhibitors.

154 line K-562 using both live-cell and in-situ cell lysis assay formats, with special focus on metallop

155 lasma membranes (based on a lysenin-mediated cell lysis assay).

156 trongly inhibited the NK cell-mediated tumor cell lysis associated with inhibition of granzyme B acti

157 tional killing assays measure average target cell lysis at fixed times and high effector:target ratio

158 Faced with hypotonic shock, to circumvent cell lysis, bacteria open large solute-passing channels

159 ficient in IKKbeta were compromised in tumor cell lysis, based on their reduced ability to phagocytiz

160 ing equilibria within cells during and after cell lysis, because sufficient cellular chaperone/chaper

161 s were independent of the cell matrix or the cell lysis buffer and were not affected by different ant

162 endations and the use of bead beating, white cell lysis buffer, and an internal control PCR.

163 ch include the appropriate type of red blood cell lysis buffer, FMO or isotype controls to identify r

164 technology and removal of detergent from the cell lysis buffer.

165 lls in this cluster do not undergo cytocidal cell lysis but harbor abundant enveloped particles withi

166 the formin hDia1 were ineffective in target cell lysis, but for distinct reasons.

167 infection of Ostreococcus tauri often causes cell lysis, but two spontaneously arising resistance mec

168 ight contribute to the sensitivity of target cell lysis by CTL.

169 de of epithelial cell plasticity on targeted cell lysis by cytotoxic T lymphocytes (CTL).

170 Understanding the mechanism of bacterial cell lysis by E will provide insights into new antimicro

171 Simian virus 40 (SV40) appears to initiate cell lysis by expressing the late viral protein VP4 at t

172 ane protein E whose expression leads to host cell lysis by inhibition of the peptidoglycan synthesis

173 Our methodology of studying E. coli cell lysis by Lysin A and its truncations after expressi

174 ing to optimal TRAIL expression and melanoma cell lysis by pDCs.

175 Triggering cell lysis by peptidoglycan synthesis inhibition is a tr

176 tive effect resulted from inhibition of host cell lysis by pneumococcal cholesterol-dependent cytotox

177 uce cell lysis, the pattern and mechanism of cell lysis can vary substantially.

178 determination of the efficiency of recipient cell lysis catalyzed by this intercellular toxin deliver

179 man endothelial cells and, in the absence of cell lysis, cause two diseases resulting from increased

180 release valve, preventing the occurrence of cell lysis caused by acute osmotic stress.

181 laser-based cellular manipulation including cell lysis, cell necrosis, and molecular delivery.

182 and 2B4 was not sufficient for strong target cell lysis, collective engagement of LFA-1, NKG2D, and 2

183 ession was lacking and no spontaneous target cell lysis could be detected in vitro, although perforin

184 Cell lysis destabilizes the apparent 60-kDa tetramer, le

185 Cell lysis, DNA extraction, and hydrolysis were accompli

186 Cell lysis, DNA fragmentation and immunoaffinity purific

187 Five others cause frequent cell lysis during cell separation and map to two loci.

188 and rga7Delta result in defective septum and cell lysis during cytokinesis.

189 ease assays, this mutant exhibited increased cell lysis during stationary phase, suggesting that olig

190 d selective cell clearance was achieved with cell lysis efficiencies of 94 and 96%.

191 ession of granzyme B-dependent CD107a and MM cell lysis, even in the presence of bone marrow stromal

192 The entire protocol including cell lysis, extraction of DNA, polymerase chain reaction

193 in bacteriophage genomes, where they promote cell lysis for virion release, and within bacterial geno

194 ogical emergency release valve that prevents cell lysis from acute osmotic stress.

195 andard biochemistry laboratory equipment for cell lysis, gel electrophoresis and western blotting.

196 We used HSV1716/NAT as a dual cell lysis-gene delivery vehicle for targeting the NAT t

197 Cell lysis genes that are induced following PrtR autocle

198 ough the exact sequence of events leading to cell lysis has not yet been completely elucidated.

199 Different mechanisms for cell lysis have been proposed, but these models tend to

200 Specifically, we used cryogenic cell lysis, immunopurifications on magnetic beads, and m

201 Even when considering the fastest phage (cell lysis in 9 minutes), the concentrations of phage-in

202 ation of removable electrodes for electrical cell lysis in a specified portion of the channel (1 mm w

203 s directly with the lipid bilayer leading to cell lysis in a strongly concentration-dependent fashion

204 d donor-type (B/c) but not third-party (C3H) cell lysis in sensitized BKO hosts.

205 equires demonstration that a small amount of cell lysis in the cellular population is not responsible

206 lly to tumor cells and led to specific tumor cell lysis in the presence of complement.

207 r cells, and such immune sera mediated tumor cell lysis in the presence of complement.

208 ecific antibody efficiently induces targeted cell lysis in the presence of effector cells at as low a

209 cytolytic function in vivo, inhibited target cell lysis in vitro, and augmented IFN-gamma responses t

210 Ab-dependent complement activation and tumor cell lysis in vitro.

211 identified a decrease in Ag-specific target cell lysis in vivo by CD8(+) T cells from GKO mice as th

212 suggested DSW stimulation of phage-mediated cell lysis, in previously infected cells.

213 Production of FFA has been shown to cause cell lysis, induce stress responses, and impair basic ph

214 pase activation as part of the mechanism for cell lysis induced by adenovirus and suggests that manip

215 metal ions into the cells and accelerate the cell lysis induced by Rituximab.

216 a a multistep process including direct tumor cell lysis, induction of cytotoxic or apoptosis-sensitiz

217 lular signaling molecule able to amplify the cell lysis inflicted by certain bacterial toxins includi

218 lease of DnaK via secretion and/or bacterial cell lysis into the extracellular milieu and inhibition

219 ion of infectious material in the absence of cell lysis is enabled by components of the autophagy pat

220 We show DNA released via cell lysis is readily available for HGT and may be parti

221 IS (icIS), which is unable to trigger target cell lysis, is loose, with multiple protrusions in the e

222 In this study, we describe a novel cell lysis mechanism for fungal and tumor cells by the p

223 n bubble dynamics that lead to laser-induced cell lysis, necrosis, and molecular delivery.

224 ytes in the airway and that significant host cell lysis occurred during early infection.

225 of cell wall synthesis and cell division and cell lysis occurred for the higher antibiotic dose.

226 ing conidial-base germ tube degeneration and cell lysis occurring during growth, a phenomenon exacerb

227 In this monolithically integrated device, cell lysis occurs at a channel intersection using a comb

228 Phage-mediated cell lysis of dual-membrane Gram-negative bacteria also

229 first evidence that the susceptibility to NK cell lysis of EBV-infected B cells undergoing lytic repl

230 -expanded Tregs, and anti-ULBP1 inhibited NK cell lysis of expanded Tregs.

231 only anti-NKG2D and anti-NKp46 inhibited NK cell lysis of expanded Tregs.

232 s composed of C5b to C9 (C5b-9) and mediates cell lysis of invaded pathogens.

233 Viral infection led to mass cell lysis of the O. tauri cells within 48 h.

234 Endolysin CD27L causes cell lysis of the pathogen Clostridium difficile, a majo

235 Overexpression of bsrE causes cell lysis on agar plates.

236 cribed mechanism is independent of explosive cell lysis or cell death, and the release of DNA is conf

237 VRE biofilms while showing minimal red blood cell lysis or cytotoxicity against HeLa cells.

238 neral phenomenon and is not a consequence of cell lysis or membrane shedding; instead, their secretio

239 nic carbon analyses suggest that products of cell lysis or microbial products released under starvati

240 s impulse J greater, similar0.1 Pa s ensures cell lysis or necrosis, whereas exposures in the range o

241 This protocol, which includes cell lysis, overnight tryptic digestion, sample analysis

242 We suggest that activation of the Alp cell lysis pathway is a disease-enhancing response to ba

243 at impaired respiration elicits a programmed cell lysis (PCL) phenomenon in S. aureus leading to the

244 ese results demonstrate that cidA-controlled cell lysis plays a significant role during biofilm devel

245 Moreover, cell lysis procedures can be hard to standardize, leadin

246 Cleavage of AlpR triggers a cell lysis program through de-repression of the alpA gen

247 thelial cell death via pyroptosis results in cell lysis, proinflammatory cytokine release and escape

248 The procedure includes cell lysis, protein precipitation using acidified methan

249 bound to viral RNA are cross-linked prior to cell lysis, purified, and identified using mass spectrom

250 her with meropenem resulted in rapid, polar, cell lysis releasing cytoplasmic contents.

251 ammation in a manner independent of MLKL and cell lysis remains unclear.

252 ough A2B5+, O4+, and O1+ complement-mediated cell lysis resulted in a delay in development of MBP cel

253 avage, cytokine activation, and, ultimately, cell lysis resulting from the formation of membrane pore

254 However, cell lysis revealed a viscoplastic response of the under

255 ster cells by micromanipulation, followed by cell lysis, reverse transcription, gene-specific cDNA am

256 zed to activate and induce tumor-directed NK cell lysis since IL-2-stimulated NK cells mediated tumor

257 nvelope and specifically eliminates, through cell lysis, sporulating cells that assemble the envelope

258 hesize that small molecules added during the cell lysis stage can yield soluble protein from insolubl

259 y plaque assay, whereas phageFISH identified cell lysis starting at < 5 h and lasting to 11 h, but fo

260 phaS in intact normal neurons, but not after cell lysis, suggesting a dynamic equilibrium.

261 ery continues moving circumferentially until cell lysis, suggesting that cross-link cleavage is not r

262 pGpp, limiting fatty acid synthesis leads to cell lysis, supporting a role for ppGpp as a linchpin li

263 d and postulated functions and uses of these cell lysis systems.

264 ion analysis of few single cells, a one-step cell lysis, target labelling and hybridisation approach

265 Unlike eDNAs from cell lysis that were abundant and mainly concentrated ar

266 Upon cell lysis, the modified drug bound to the protein is pu

267 biosynthesis is generally thought to induce cell lysis, the pattern and mechanism of cell lysis can

268 Starting from cell lysis, this protocol can be completed in approximat

269 uble and membrane-associated fractions after cell lysis, though only the latter appeared to be in a n

270 n recent experimental observations of single-cell lysis times in bacteriophage [Formula: see text] He

271 ctivity buffer expedites the transition from cell lysis to protein electrophoresis.

272 The timescale from cell lysis to purified protein sample is 5-6 d.

273 d other metabolites, likely released through cell lysis, to supplement metabolic pathways.

274 ve therapeutic combination of tumor-specific cell lysis together with immune stimulation, therefore a

275 is localized to the pole and does not cause cell lysis under physiological conditions.

276 action of viruses, once they are released by cell lysis, undergo fast decomposition.

277 chains for phospholipid synthesis results in cell lysis unless RpfB is present to counteract the RpfF

278 ese channels act as safety valves preventing cell lysis upon hypoosmotic cell swelling: the channels

279 y active domains, both of which show E. coli cell lysis upon their expression exclusively in the peri

280 ults indicate that anti-B5 antibody-directed cell lysis via complement is a powerful mechanism for cl

281 ring of the complement cascade induces tumor cell lysis via complement-dependent cytotoxicity (CDC) a

282 Impaired respiration led to increased cell lysis via divergent regulation of two processes: in

283 evealed that Fn14 dimerization occurs during cell lysis via formation of an intermolecular disulfide

284 tides that were capable of inhibiting target cell lysis via interaction with CD94-NKG2A, yet had litt

285 Prior to cell lysis, virus infection resulted in the formation of

286 To investigate the contribution of VP4 to cell lysis, VP4 was expressed in mammalian cells where i

287 In separate experiments, efficient tumor cell lysis was achieved in vitro at drug concentrations

288 n), target cell conjugation, and K562 target cell lysis was compared between mutant- and wild-type-tr

289 Extracellular DNA (eDNA), a by-product of cell lysis, was recently established as a critical struc

290 lyzed (either IFN-gamma production or target cell lysis), we wondered whether monitoring for multiple

291 d to prevention of macrophage activation and cell lysis, we suggest that the molecular environment su

292 Swab extraction and cell lysis were accomplished using magnetic-driven agita

293 vitro and levels of human MAC deposition and cell lysis were measured.

294 on yielded increased NK cell-mediated target cell lysis when compared with rituximab alone.

295 efects and formed membrane blebs that led to cell lysis when GlcNAc was replaced by chitobiose in the

296 ation and was associated with an increase in cell lysis, which was suppressed by a calcium-dependent

297 cles are released primarily by virus-induced cell lysis, while in insect cells they bud from the plas

298 high-quality RNA requires the use of direct cell lysis with a phenol guanidine-based reagent or an a

299 ystem designed to cause programmed bacterial cell lysis with no survivors.

300 Because cell lysis would be detrimental to epithelial nitric oxi