ライフサイエンスコーパス: cell plate

1 ontrols (185 +/- 32 v 40 +/- 3 per 2 x 10(5) cell plated).

2 ant numbers of colonies (11-50 colonies/1000 cells plated).

3 icles to the CPAM and thereby to the growing cell plate.

4 the cytoplasm to the expanding and maturing cell plate.

5 on the phragmoplast microtubules and on the cell plate.

6 , which is required for the formation of the cell plate.

7 for the synthesis of callose at the forming cell plate.

8 which later become integrated into the main cell plate.

9 of vesicle-tubule-vesicle structures at the cell plate.

10 ly localized to microtubules near the future cell plate.

11 e-vesicle structures observed on the forming cell plate.

12 ules are located near the site of the future cell plate.

13 e formation, and often results in an oblique cell plate.

14 redistributed to the growing margins of the cell plate.

15 d across the whole width of the newly formed cell plate.

16 tein is associated with the formation of the cell plate.

17 at directs vesicles secretion to the nascent cell plate.

18 the site where they coalesce to form the new cell plate.

19 sin form positively charged scaffolds in the cell plate.

20 molecules involved in the maturation of the cell plate.

21 nd membrane and callose are deposited at the cell plate.

22 targeting to the outward-growing rim of the cell plate.

23 d formed dense tubule-like structures in the cell plate.

24 cipates in the construction of newly forming cell plates.

25 ein) fusion protein was localized to growing cell plates.

26 ion and sorted by flow cytometry into single cell plates.

27 imals averaged 8.23 +/- 3.3601 per 3 million cells plated.

28 +/- 2.3523 osteogenic colonies per 3 million cells plated.

29 plus ends to deliver materials bound for the cell plate [2] [3].

30 esion molecule antibodies, and of 144 single cells plated, 39 clones were expanded, propagated, and s

31 The formation of the cell plate, a unique structure in dividing plant cells,

32 t most of its somata are confined to a dense cell plate adjacent to the fourth ventricle.

33 of defects, including branched or irregular cell plates, altered Golgi morphology and ectopic callos

34 re laterally displaced, and that the growing cell plate anchors on one side of the cell at an early s

35 Aurora2) associate with the spindle and the cell plate and are implicated in controlling formative d

36 mutant, reduced transport of vesicles to the cell plate and formed dense tubule-like structures in th

37 The localisation of NPK1 to the cell plate and its mitosis-specific activation suggest t

38 Callose is synthesized on the forming cell plate and several other locations in the plant.

39 his protein is involved in maturation of the cell plate and the re-establishment of cytoplasmic actin

40 Immunolabeling experiments show that the cell plates and cell walls of the endosperm differ from

41 rotein located in the nascent cross wall or "cell plate" and also in mature cell walls.

42 to appear first in the center of the forming cell plate, and as the cell plate grew outward, it redis

43 ls enhanced callose synthesis on the forming cell plate, and that these cell lines exhibited higher l

44 ective microspores did not form a continuous cell plate, and two identical nuclei were produced with

45 plant cytokinesis, including assembly of the cell plate, are not fully understood.

46 required to initiate, assemble and shape the cell plate as it grows toward the mother cell cortex is

47 CD34(+)/c-kit(+)/CD13(+) cells plated as single cells in the presence of various

48 Clonogenic (single-cell plating) assays were used to define and quantify su

49 nsisting of a filamentous ribosome-excluding cell plate assembly matrix (CPAM) and Golgi-derived vesi

50 ween vesicles and regulatory proteins at the cell plate assembly matrix during polysaccharide deposit

51 At their equatorial planes, cell plate assembly sites are formed, consisting of a fi

52 l1A-2 adl1E-1 double mutants show defects in cell plate assembly, cell wall formation, and plasma mem

53 s matrix contains the molecules that mediate cell plate assembly.

54 ning between daughter cells as well as rapid cell plate assembly.

55 aracterization of patellin1 (PATL1), a novel cell-plate-associated protein that is related in sequenc

56 CalS1 interacts with two cell plate--associated proteins, phragmoplastin and a no

57 o constrict the ER, was not recruited to the cell plate at cytokinesis.

58 ent in the PD proteome, are recruited to the cell plate at late telophase, when primary PD are formed

59 We show that NCS cells plated at clonal density give rise to multiple neu

60 higher functional HIF-1alpha expression than cells plated at high density independent of O2 tension.

61 to ICE family proteases or by aggregation of cells plated at high density.

62 ed at lower densities release more PGE2 than cells plated at higher densities.

63 the keratocyte markers in 7-day cultures in cells plated at low (5,000 cells/cm2) and high (20,000 c

64 Inhibition of FAK or Src in MCF-10A cells plated at low cell density prevented the activatio

65 ous manner, as indicated by its detection in cells plated at low density and in cultures in which dep

66 Human prostate cancer cells plated at low density manifested higher functional

67 Cells plated at lower densities release more PGE2 than c

68 , are essential for polar cell expansion and cell plate biogenesis.

69 t to the involvement of phosphoinositides in cell-plate biogenesis.

70 nts, including surface functionalization and cell plating, can be completed in 10 h.

71 at is required for callose deposition at the cell plate, cell wall and plasmodesmata.

72 n the recruitment or removal of a variety of cell plate components; thus, they did not demonstrate a

73 e was enhanced by low doses of inhibitors of cell plate consolidation and vesicle secretion.

74 lagenase stimulatory activity and epithelial cell plating density was demonstrated.

75 se B by these cells is similarly affected by cell plating density.

76 In contrast to this picture, we observe that cell-plate development in Arabidopsis shoot cells is hig

77 ntly from primary cultures of dissociated TG cells plated directly after removal from the mouse (7 an

78 essential for the correct positioning of the cell plate during cytokinesis in cells of the developing

79 ily of proteins, becomes associated with the cell plate during cytokinesis in plants.

80 ic protein is functional and targeted to the cell plate during cytokinesis in transgenic cells.

81 In higher plants, the formation of the cell plate during cytokinesis requires coordinated micro

82 h in turn direct the vesicles to the forming cell plate during cytokinesis.

83 rent patterns of localization at the forming cell plate during cytokinesis.

84 es in interphase cells and with the immature cell plate during cytokinesis.

85 T1 fusion protein was located on the forming cell plate during cytokinesis.

86 Construction of the cell plate during plant cell division requires the preci

87 rafficking is central to construction of the cell plate during plant cytokinesis.

88 that orchestrate the growth and guidance of cell plates during cytokinesis.

89 plane that minimizes the surface area of the cell plate (Errera's rule) while creating daughter cells

90 d distinguished three distinct phases during cell plate expansion in tobacco (Nicotiana tabacum) 'Bri

91 membrane-trafficking events associated with cell-plate expansion or maturation and point to the invo

92 like other gsl8 mutants, in which defects in cell plate formation are seedling lethal, cytokinetic de

93 een isolated and shown to be associated with cell plate formation in soybean by using immunocytochemi

94 ragmoplastin, it appears that the process of cell plate formation is completed in two phases.

95 pan1 mutants exhibited no defects in cell plate formation or in the recruitment or removal of

96 Cell plate formation within the CPAM appears to be initi

97 ant negative, slowing down the completion of cell plate formation, and often results in an oblique ce

98 accurate phragmoplast fusion, and subsequent cell plate formation, at the preprophase band site.

99 cles, which accumulate at the early stage of cell plate formation, were not affected by ES7, KNOLLE w

100 trafficking and fusion machinery involved in cell plate formation.

101 wn about the forces that model the ER during cell plate formation.

102 tes in an early membrane fusion event during cell plate formation.

103 s to monitor the dynamics of early events in cell plate formation.

104 esis in the microspore that is essential for cell plate formation.

105 provide a mechanically robust mechanism for cell-plate formation in large cells and suggests a simpl

106 Here we show that membranous material for cell-plate formation initially accumulates along regions

107 k for how the cytoskeleton spatially defines cell-plate formation is lacking.

108 smata (PD) arise at cytokinesis when the new cell plate forms.

109 novel kind of cell plate, the syncytial-type cell plate, from Golgi-derived vesicles approximately 63

110 callose persists in the cell walls after the cell plates fuse with the parental plasma membrane.

111 During cytokinesis, the expanding cell plate fuses with the plasma membrane at the cortica

112 heral cell plate growth zone, which leads to cell plate fusion with the cell wall.

113 center of the forming cell plate, and as the cell plate grew outward, it redistributed to the growing

114 maximum during the late PFS stage, when most cell plate growth is completed.

115 eates the centrifugally expanding peripheral cell plate growth zone, which leads to cell plate fusion

116 tubular networks, giving rise to new foci of cell plate growth, which later become integrated into th

117 s suggested to be responsible for regulating cell plate growth.

118 ence microscopy, NSPN11 was localized to the cell plate in dividing cells.

119 tin has been shown to be associated with the cell plate in dividing cells.

120 punctate subcellular structures and with the cell plate in dividing cells.

121 fluorescence microscopy localized PDL to the cell plate in dividing soybean root tip cells.

122 t cell is accomplished by the formation of a cell plate in the center of the phragmoplast.

123 PAN1, but not PAN2, is localized to cell plates in all classes of dividing cells examined.

124 e-dimensional architecture of syncytial-type cell plates in the endosperm of Arabidopsis has been ana

125 Single cell plating in serum-free medium allows direct assessme

126 observed spread of infection to nonneuronal cells plated in a different compartment.

127 aggregated fail to differentiate, but mutant cells plated in a wild-type background are able to do so

128 In contrast, the substratum of subconfluent cells plated in noncoated dishes lacked vitronectin but

129 ng term bone marrow cultures, or bone marrow cells plated in semisolid medium.

130 For these determinations, cells plated in serum-free medium were treated either wi

131 e phosphorylation of VEGFR-2 was observed in cells plated in sparse culture conditions (60-65% conflu

132 servation of elevated levels of p27(KIP1) in cells plated in the presence of fibrillar collagen has l

133 Cells plated in the presence of fibrillar collagen were

134 (KOR) gene causes the formation of aberrant cell plates, incomplete cell walls, and multinucleated c

135 Cell plates induced to insert outside the predicted divi

136 t cytoskeleton has depolymerized and the new cell plate is beginning to mature.

137 The cell plate is formed by the fusion of Golgi apparatus-de

138 aped, membrane-enclosed precursor termed the cell plate is formed that radially expands toward the pa

139 distribute around the phragmoplast where the cell plate is formed.

140 that polarized targeting of KOR1 to forming cell plates is essential for cytokinesis.

141 d with exocytic vesicles that are depositing cell plate material during cytokinesis in the plant cell

142 al role of callose during the late stages of cell plate maturation and establish the temporal relatio

143 tions of secretory and endosomal vesicles in cell plate maturation.

144 t recycling at the PM, vacuolar sorting, and cell-plate maturation.

145 dopsis RanGAP1 is associated with the NE and cell plate, mediated by an N-terminal, plant-specific WP

146 Without shortening, the wider cell-plate membrane depositions evolved into cell walls

147 A ribosome-excluding matrix encompasses the cell plate membranes from the fusion of the first vesicl

148 ctopic expression, ARL2 is also found at the cell plate of dividing cells during cytokinesis, an area

149 opy indicates that ADL1C is localized to the cell plate of dividing somatic cells and to the tip of e

150 A cell plated on a two-dimensional substrate forms adhesio

151 f a Boyden chamber and the rest of the liver cells plated on a cell culture insert, and fish oil or f

152 Whether and how cells plated on a two-dimensional matrix or embedded in

153 ubcB-null cells plated on agar form mounds with normal kinetics; h

154 ACh withdrawal was significantly smaller in cells plated on an alpha beta 1-integrin antibody (10 +/

155 -induced stimulation of ICa,L was greater in cells plated on an alphabeta1-integrin antibody than on

156 Cleavage of RhoA was detectable in BAE cells plated on an integrin substrate; it did not occur

157 stiffness at the cellular level for MC3T3-E1 cells plated on biomaterial substrates of varying modulu

158 Only RPE cells plated on cleaned or cleaned and ECM-coated ICL de

159 nin-5, and had no effect on reaggregation of cells plated on collagen I (alpha2beta1 integrin ligand)

160 However, endothelial cells plated on collagen I were resistant to TGF-beta1-i

161 Human umbilical vein endothelial cells plated on collagen I-coated plates and cultured in

162 lphavbeta5 inhibited degradation (70-90%) by cells plated on collagen or laminin.

163 A is essential for the active stiffening of cells plated on collagen-coated substrates.

164 ls plated on fibronectin but 0.35 ng/min for cells plated on collagen.

165 onized with IgG or complement, compared with cells plated on control protein.

166 f CLANs and quantitate CLAN formation in HTM cells plated on coverslips coated with various extracell

167 Analyses of invadopodia function from cells plated on cross-linked gelatin and collagen gels u

168 n, using live-cell imaging of invadopodia in cells plated on cross-linked gelatin, was consistent wit

169 trap micrometer-sized beads internalized in cells plated on crossbow-shaped adhesive micropatterns a

170 Moreover, cells plated on extracellular matrix-coated coverslips s

171 Activation of PAK is higher in cells plated on fibronectin (FN) compared to basement me

172 [125I]vitronectin, which was 3.0 ng/min for cells plated on fibronectin but 0.35 ng/min for cells pl

173 f focal adhesions and actin stress fibers by cells plated on fibronectin depends on adhesion-mediated

174 Whereas endothelial cells plated on fibronectin or fibrinogen activate NF-ka

175 eta 1-containing sites of focal adhesions on cells plated on fibronectin or the III-9,10 modules of f

176 Translocation of YopH into cells plated on fibronectin resulted in rapid and select

177 In cells plated on fibronectin, FAK could indeed autophosph

178 combined effect 70%) of [125I]vitronectin by cells plated on fibronectin, only mAb anti-alphavbeta5 i

179 ignificantly retarded the spreading of REF52 cells plated on fibronectin.

180 phosphorylation in alpha(5)-deficient muscle cells plated on fibronectin.

181 reased IGFBP-5 gene expression 3-fold in the cells plated on fibronectin.

182 ere continuously elevated in PRL-1 knockdown cells plated on fibronectin.

183 uced here showed that mu1B-knocked down MDCK cells plated on filters at confluency and cultured for 4

184 ntegrins into focal contacts in alpha 5-null cells plated on FN, indicating that alpha V integrins ca

185 phosphorylation of Tyr(397) to match that of cells plated on FN.

186 In contrast, FN-null cells plated on FNIII(8-11) contiguous with FN-GFBD surv

187 Cells plated on glucose-modified collagen IV showed redu

188 ces of spreading and differentiation of stem cells plated on hydrogel and silicone gel substrates on

189 fibers indistinguishable from those seen in cells plated on intact fibronectin.

190 , elicited a greater stimulation of ICa,L in cells plated on laminin (+79 +/- 16 %; n = 17) than on g

191 ith 50 microM cAMP was not different between cells plated on laminin or glass.

192 ed cAMP content was significantly smaller in cells plated on laminin than on glass.

193 olin (1 microm) was significantly smaller in cells plated on laminin than on glass.

194 SCLC cells plated on laminin were not only resistant to serum

195 o-localize in retraction fibers in carcinoma cells plated on laminin, a finding suggesting a function

196 Cells plated on laminin-5 for 16 d express increased lev

197 Mouse mesangial cells plated on MGO-modified collagen IV showed decrease

198 defines the rear instead of the front, using cells plated on micropatterned adhesive strips to facili

199 tion were impaired in migrating cells and in cells plated on micropatterned substrates, and cell migr

200 Cells plated on monoclonal antibody 281.2 initially exte

201 n was cell substrate-dependent since type II cells plated on plastic dishes did not show this effect.

202 Cells plated on PN form fewer stress fibers and are more

203 ial restoration of cell cycle progression in cells plated on poly-L-lysine, providing further support

204 n an integrin substrate; it did not occur in cells plated on poly-l-lysine.

205 ression of FAK in serum-starved glioblastoma cells plated on recombinant (rec)-osteopontin resulted i

206 FAK further enhances invadopodia activity in cells plated on rigid polyacrylamide substrates.

207 Nonpigmented cells plated on soft collagen gels retained a rounded sh

208 In experiments on human mesenchymal stem cells plated on soft, medium rigidity, and hard silicone

209 activates specific integrins in endothelial cells plated on substrates containing the cognate extrac

210 ed 70-kDa fragments to cycloheximide-treated cells plated on the 160-kDa substrate, suggesting that a

211 emotaxis induced by PDGF-BB were enhanced in cells plated on the alphavbeta3 ligand vitronectin compa

212 alphavbeta3 ligand vitronectin compared with cells plated on the beta1 integrin ligand collagen.

213 f focal adhesions and actin stress fibers in cells plated on the cell-binding domain of fibronectin c

214 protein levels were very low in endothelial cells plated on the non-integrin cell attachment factor,

215 beta 1/TM4 complexes toward the periphery of cells plated on various extracellular matrix substrates

216 RPE cells plated onto BL repopulated the explant surface wit

217 Cells plated onto collagen or laminin, which engage diff

218 xplant surface within 14 +/- 3 days, whereas cells plated onto the ICL and EL eventually died and nev

219 In contrast, cells plated onto the laminin-5-rich matrices of pp126 e

220 Human corneal endothelial cells plated onto the matrices elaborated by bovine corn

221 Further analysis of the cells plated onto vitronectin indicated that PDGF stimul

222 increased in proportion to the number of MMQ cells plated out.

223 e, based on their prolonged staining for the cell-plate polymer callose.

224 ilar polarity developed in phragmoplasts and cell plates, raising the possibility that polarized divi

225 The CPAM, which is found only around growing cell plate regions, is suggested to be responsible for r

226 fferentiated root cells and targeting to the cell plate remain intact.

227 Growth of the cell plate requires continuous fusion of vesicles, and p

228 ts of the dynamics of vesicle markers on the cell plate revealed an overall reduction of the delivery

229 ates the deposition of callose at developing cell plates, root hairs, and plasmodesmata.

230 An Arabidopsis gene encoding a putative cell plate-specific callose synthase catalytic subunit (

231 The interplay between cell plate-specific post-Golgi vesicle traffic and callo

232 most well-characterized component, KNOLLE, a cell plate-specific soluble N-ethylmaleimide-sensitive f

233 That CalS1 is a cell plate--specific enzyme is demonstrated by the obser

234 e mechanism ensuring the maturation of those cell plates successfully contacting the "programmed" cor

235 abolish targeting to the nuclear rim and the cell plate, suggesting that the same mechanism is involv

236 mitotic cells and their localisation to the cell plate suggests a role in cytokinesis.

237 h the phragmoplast initials and with the TVN cell plate that is formed within the solid phragmoplast.

238 fusion protein was localized at the growing cell plate, that expression of CalS1 in transgenic tobac

239 e mini-phragmoplasts produce a novel kind of cell plate, the syncytial-type cell plate, from Golgi-de

240 rane may play important roles in guiding the cell plate throughout much of its development.

241 hind by the PPB and is proposed to guide the cell plate to the cortical division site is unknown.

242 vo and colocalize with RanGAP1 at the NE and cell plate were identified.

243 LBs are mobile at the edge of the developing cell plate, where new wall materials are being delivered

244 he plant-specific cytokinetic organelle, the cell plate, which develops across the division plane and

245 ssemble a new intracellular compartment, the cell plate, which grows centrifugally by vesicle fusion

246 sis was abolished without the formation of a cell plate, which led to failures in the birth of the ge

247 ndocytic membrane remodeling in the maturing cell plate while the plate is stabilized by callose.

248 simple PDs are inserted into the developing cell plate, while during wall extension, more complex (b

249 r at the end of interphase and predict where cell plates will fuse with parental walls during divisio