ライフサイエンスコーパス: cell separation

1 delay at the terminal stage of cytokinesis (cell separation).

2 c kinase that acts as a spatial inhibitor of cell separation.

3 cytoplasmic compartmentalization and (viii) cell separation.

4 ced by the divisome must be split to promote cell separation.

5 sion of the outer membrane (OM) and daughter cell separation.

6 e four genes produces only a modest delay in cell separation.

7 he cleavage of connective septal PG to allow cell separation.

8 trafficking of cargo molecules required for cell separation.

9 of LytF that is necessary and sufficient for cell separation.

10 lysis of septal peptidoglycan, which enables cell separation.

11 equired for septal PG splitting and daughter cell separation.

12 -regulated genes involved in mother-daughter cell separation.

13 s that function in cell wall remodelling and cell separation.

14 murein amidases, AmiD did not participate in cell separation.

15 with the major peptidoglycan amidases during cell separation.

16 t caps is induced in correlation with border cell separation.

17 cytokinesis, including septum formation and cell separation.

18 ter cell-specific transcription required for cell separation.

19 cts and disassembles, resulting in delays in cell separation.

20 resolve membrane connections to bring about cell separation.

21 gions that act in concert to ensure complete cell separation.

22 low outer membrane constriction and daughter cell separation.

23 tereotypical manner, which leads to daughter cell separation.

24 to the target cell, thus allowing effective cell separation.

25 e abnormal thick septa leading to defects in cell separation.

26 utants, mid2delta mutants had delays in cell-cell separation.

27 Skp1 homologue and the F-box protein Pof6 in cell separation.

28 rm the cleavage furrow and complete daughter cell separation.

29 ntly to support transcription and facilitate cell separation.

30 ibility to the L domain and subsequent virus-cell separation.

31 lar bud site selection in diploid cells, and cell separation.

32 EN gene MOB1 is required for cytokinesis and cell separation.

33 her and daughter cells after cytokinesis and cell separation.

34 used additional constrictions and a delay in cell separation.

35 the bud neck in late anaphase, just prior to cell separation.

36 ing pathways to regulate cell morphology and cell separation.

37 ce, spindle formation, nuclear division, and cell separation.

38 f1p splits into two rings, disappearing upon cell separation.

39 and septum synthesis and disappears prior to cell separation.

40 It took as little as 5 min to achieve cell separation.

41 sion and also a defect in cytokinesis and/or cell separation.

42 TGFbeta2 antibody inhibited endothelial cell-cell separation.

43 le revealed that Pob1p is also essential for cell separation.

44 fect is in cytokinesis, septum formation, or cell separation.

45 the cell surface and inhibition of daughter cell separation.

46 f secretion to the bud neck is necessary for cell separation.

47 roper execution of cytokinesis and efficient cell separation.

48 is utilized to achieve the DEP-based on-chip cell separation.

49 ed in the floral abscission zone just before cell separation.

50 ntify the impact of the micromagnets on rare cell separation.

51 pathways to regulate septum formation and/or cell separation.

52 tin rings on mother and daughter cells after cell separation.

53 rphase through to the contractile ring until cell separation.

54 nd vesicle trafficking in the later steps of cell separation.

55 ed in cell cycle control and mother-daughter cell separation.

56 by target enzymes called amidases to promote cell separation.

57 in, suggesting they are limiting factors for cell separation.

58 n assembly required for septum synthesis and cell separation.

59 al forces of the cell turgor pressure during cell separation.

60 luding cell expansion, organ initiation, and cell separation.

61 nctions of alpha(1-3)glucan in septation and cell separation.

62 ign and operation of size-based microfluidic cell separation.

63 plications such as regenerative medicine and cell separation.

64 ther with synthases, for growth and daughter cell separation.

65 esions with enhanced migration and transient cell separation.

66 enzymes called "amidases" to drive daughter-cell separation.

67 cruit EnvC to the septum but fail to promote cell separation.

68 luidic channel was developed for high-purity cell separations.

69 aling occurs for those cells at smaller cell-cell separations.

70 gene receptor cells (U87EGFRvIII) at varying cell separations.

71 rmed multicellular clumps through incomplete cell separation, 10 increased invertase expression, none

72 ings appear to be involved primarily in cell-cell separation, a late stage in cytokinesis.

73 nt skin disorder characterized by suprabasal cell separation (acantholysis) of the epidermis.

74 Upon cell separation after cytokinesis, the new cell ends ado

75 Conventional cell separation against multiple markers generally requi

76 l wall hydrolases (amidases) responsible for cell separation (AmiA, AmiB and AmiC).

77 -acetylmuramyl-l-alanine amidase involved in cell separation (AmiC), as compared with three largely r

78 y, and we provided the first evidence that a cell separation amidase can utilize a small synthetic PG

79 results thus suggest that the order in which cell separation amidases and their activators localize t

80 C), as compared with three largely redundant cell separation amidases found in Escherichia coli (AmiA

81 her than the zinc cofactor typically used by cell separation amidases, potentially protecting its abi

82 r to further analyze the process of root cap cell separation and a root cap specific promoter for tar

83 shortly after it is made to promote daughter cell separation and allow outer membrane constriction to

84 ssion by LexA-Sin3 and also cause defects in cell separation and altered colony morphology.

85 N. gonorrhoeae results in severely impaired cell separation and altered peptidoglycan (PG) fragment

86 l-length AtlA protein did not restore normal cell separation and biofilm formation.

87 l-wall processing during the growth and cell-cell separation and designated the gene as cell-division

88 Plant cell separation and expansion require pectin degradation

89 f using microelectronic chip arrays for both cell separation and gene expression profiling provides a

90 ing root tissues by inducing auxin-dependent cell separation and hydraulic changes in adjacent cells.

91 Five others cause frequent cell lysis during cell separation and map to two loci.

92 Cell separation and mixing experiments demonstrated that

93 Disruption of the spm1+ gene interferes with cell separation and morphogenesis.

94 ual motile cells in which genes for daughter cell separation and motility are ON, and chains of sessi

95 remains locked in a high SlrR state in which cell separation and motility genes are OFF for extended

96 tic switch for controlling genes involved in cell separation and motility.

97 ins binds to and represses genes involved in cell separation and motility.

98 induction by auxin is required to coordinate cell separation and organ emergence.

99 tial, this activity is critical for daughter cell separation and outer membrane invagination during d

100 cell wall constituents that are critical for cell separation and pathogenesis.

101 hat increased AmiC activity also resulted in cell separation and PG fragment release defects, indicat

102 evealed parameters for how AmiC functions in cell separation and PG fragment release.

103 activity but is only partially required for cell separation and polarized growth.

104 hat flagella-based forces initiated daughter cell separation and provided a source for membrane tensi

105 ce of the flow-through centrifuge applied to cell separation and resuspension and to DNA purification

106 sorter, we demonstrate simultaneous on-chip cell separation and sizing with three different samples

107 his patterned thermoresponsive films enables cell separation and sorting by modulating temperature- a

108 led that Glu-229 is critical for both normal cell separation and the release of PG fragments by gonoc

109 ded cells, migrated to the bud neck prior to cell separation and then rapidly relocalized to the inci

110 r phenotypes observed were lysis, failure of cell separation and/or cytokinesis, impaired bud growth

111 n increases sigmaD-directed gene expression, cell separation, and autolysis.

112 Both ace2Delta and ace2-35 show defects in cell separation, and both can rescue the growth defects

113 logue Cbk1, which controls polarized growth, cell separation, and cell integrity.

114 ime- dependent destruction of actin bundles, cell separation, and cell loss.

115 tip, relocates to the mother-bud neck before cell separation, and finally migrates to the incipient b

116 CD34(+) cells were isolated by magnetic cell separation, and high-density oligonucleotide microa

117 ork are required for cellular morphogenesis, cell separation, and maintenance of cell integrity.

118 of settings such as thrombosis, immunology, cell separations, and diagnostics.

119 While cell purity was >95% using both cell separation approaches, gene expression analysis rev

120 hanisms of apple cortex textural changes and cell separation are discussed.

121 nd that the periplasmic amidases that aid in cell separation are minor players, cleaving only one-six

122 ive in DNA segregation and the completion of cell separation, are motile and still fail to localize D

123 n and chain formation, a hallmark of progeny cell separation arrest.

124 uggest that YneA is able to inhibit daughter cell separation as well as septum formation.

125 on events associated with the completion of cell separation as well as the gain of motility.

126 Inactivation of sceD resulted in impaired cell separation, as shown by light microscopy, and "clum

127 er describes central events of budding yeast cell separation, as well as the control pathways that in

128 In vitro cell separation assays show that structural proteins, pe

129 motility and decreased dynamics of transient cell separations associated with cleft formation; inner

130 the mutant cells became apparent by impaired cell separation at the end of cell division and by resis

131 Our data suggest the great potential of cell separation based on conductivity-induced DEP using

132 with the use of techniques for magnetic bead cell separation based on expression of these 3 markers.

133 Cell separation based on microfluidic affinity chromatog

134 tal analysis by magnetophoresis and magnetic cell separation based upon differences in intracellular

135 (AtlA) was recently shown to be involved in cell separation, biofilm formation, and autolysis.

136 lta cells reveals that Ace2p is required for cell separation but not for polarized growth.

137 aughter-specific gene products contribute to cell separation by degrading the cell wall.

138 serial thin-section electron microscopy, and cell separation by protoplasting.

139 (L) budding domains mediate efficient virus-cell separation by recruiting host ESCRT and ESCRT-assoc

140 oteins with LytM domains also participate in cell separation by stimulating amidase activity.

141 ynthesis or remodeling, which in turn affect cell separation, cell envelope integrity, and vibrioid m

142 lished that ethylene and auxin contribute to cell separation control.

143 , the normal mechanisms of yeast budding and cell separation create permanent scars which expose suff

144 o1p deficiency in yeast (myo1Delta) causes a cell separation defect characterized by the formation of

145 This latter phenotype leads to a cell separation defect in ndc10-1 cells.

146 or the essential pof6 gene display a similar cell separation defect noted in skp1 mutants, and Pof6 l

147 lay disorganized, diffuse septin rings and a cell separation defect similar to septin deletion strain

148 In addition, we observe an enhanced cell-separation defect in a myo1spa2 strain at 37 degree

149 more, the phosphomimic mutation can suppress cell separation defects of hgc1.

150 escue the lysis but not the cell polarity or cell separation defects of ramDelta cells.

151 inactivation of Mcs6 in csk1(+) cells causes cell separation defects or growth arrest, respectively,

152 ble mutant showed no growth abnormalities or cell separation defects, suggesting that these enzymes a

153 deleted or overexpressed have septation and cell separation defects.

154 emonstrate the efficiency of marker-specific cell separation, DEP-activated cell sorting (DACS) was a

155 rmodynamics-derived approach we analyzed the cell-separation dependence of the signaling stability, a

156 ular, cell-cell signaling can depend on cell-cell separation distance and can influence cellular arra

157 ese trajectories to identify a range of cell-cell separation distances where the signaling was most s

158 enerates a special and unique side-explosive cell separation due to an instantaneous primary septum t

159 m-positive cell wall at the site of daughter cell separation during cell division.

160 ibutes to cell wall disassembly occurring in cell separation during fruit abscission, but its role, i

161 ggests a new mechanism for the regulation of cell separation during the M/G(1) phase transition.

162 found to play crucial roles in P. aeruginosa cell separation, envelope integrity and antibiotic resis

163 l organs abscise after pollination, and this cell separation event is controlled by the peptide INFLO

164 ase is an important but poorly characterized cell separation event.

165 degradation of pectins is required for many cell separation events in plants, but the role of pectin

166 Cell separation experiments revealed that the nonparench

167 Cell separation experiments suggest that IL-10 can effec

168 lts will enable design of practical particle/cell separation, filtration, and focusing systems for cr

169 ndent pathway that is required for efficient cell separation following cytokinesis.

170 by electron microscopy indicated a defect in cell separation following mitosis.

171 ring constriction, septation, and subsequent cell separation following ring disassembly.

172 nt passive marker in label-free particle and cell separation for chemical, biomedical, and environmen

173 cribe a modified protocol for immunomagnetic cell separation for efficient isolation of human periphe

174 In the realm of cell separation for screening, there has been significan

175 umber of attributes that can be utilized for cell separation, for example, cell shape, cytoskeletal p

176 evaluate the efficacy of immunomagnetic rare cell separation from non-Newtonian particulate blood flo

177 om an endothelial cell surface layer and red cell separation from the endothelial cell surface were m

178 aromyces cerevisiae prevents mother-daughter cell separation, generating multicellular 'snowflake' ye

179 Importantly, application of cell separation, genome-wide expression, and cell-specif

180 re is a sense that the field of microfluidic cell separation has achieved a high level of maturity ov

181 Magnetic cell separation has become a popular technique to enrich

182 hnological work associated with microfluidic cell separation has been driven by needs in clinical dia

183 Immunomagnetic cell separation has been shown to be a highly attractive

184 The reported cell separation has the potential to impact the forensic

185 morphogenetic abnormalities and a defect in cell separation; however, remarkably, cytokinesis appear

186 mpartments) occurring 18 min before daughter cell separation in a 135-min cell cycle so the two const

187 mising applications of the DEP separator for cell separation in a continuous mode.

188 g furrow ingression, membrane resolution and cell separation in budding yeast.

189 Though initially designed for cell separation in DNA sequencing protocols, the flow-th

190 reakage in Kanzi apples and, in contrast, by cell separation in Golden Delicious apples.

191 his allows high-throughput dielectrophoretic cell separation in high conductivity, physiological-like

192 , both spatially and temporally, with border cell separation in pea root caps.

193 w O(2) inhibited root development and border cell separation in pea seedlings.

194 Thus, the ultrafast daughter cell separation in S. aureus appears to be driven by acc

195 and divide adjacent to the previous site of cell separation, in response to a cell-division remnant,

196 hree dimensions, resulting, after incomplete cell separation, in the 'bunch of grapes' cluster organi

197 ge cross section, together with differential cell separation, indicate the presence of europium parti

198 Impeded cell separation is accompanied by abnormalities in myosi

199 or multinucleate phenotype, indicating that cell separation is also inhibited.

200 For lymphocytes, cell separation is best achieved by fluorescence activat

201 Swift and efficient cell separation is difficult and a major hurdle for isot

202 Cell separation is mediated by autolysins, whose genes a

203 r results therefore support a model in which cell separation is stimulated by the reversible relief o

204 One important aspect of magnetic cell separation is the degree to which a cell binds para

205 ct chitin synthase that acts during or after cell separation, is transported normally in chs6 mutants

206 anges in ethylene sensitivity, including the cell separation layer throughout tomato flower abscissio

207 ession of this murein hydrolase activity and cell separation levels to those of the wild-type strain.

208 In this work, we report a scaled, label-free cell separation mechanism called non-equilibrium inertia

209 We previously reported a cell separation method that uses the fluorescence-activa

210 The study presents a dielectrophoretic cell separation method via three-dimensional (3D) nonuni

211 The cell separation method we employed has, as its final ste

212 s an efficient, high-throughput microfluidic cell separation method.

213 We present a dielectrophoresis (DEP)-based cell-separation method, using 3D electrodes on a low-cos

214 Current affinity-based protein or cell separation methods use binary 'bind-elute' separati

215 In model 2, a cell separation model, the mRNA levels of these inflamma

216 genes overlapped significantly with those of cell separation mutants sep10 and sep15.

217 Currently, cell separation occurs almost exclusively by density gra

218 ng 4 (VPS4) follows CHMP4B to this site, and cell separation occurs immediately.

219 RP is crucial to ensure that auxin-regulated cell separation occurs solely along their shared walls.

220 proteins with specific functions to support cell separation of vegetative bacilli and growth in infe

221 od for extraction of NAD(P)(H) from cultured cells, separation of analytes by capillary electrophores

222 tance of considering the potential effect of cell separation on gene expression as well as DNA methyl

223 Following cell separation on the microdevice, DNA extraction, ampl

224 equired for motility but not at the level of cell separation or flagellum biosynthesis.

225 It can be scaled up for routine laboratory cell separation or implemented on a miniaturized scale.

226 During filamentous growth, no cell separation or subsequent beta-glucan exposure occur

227 Cell separation, or abscission, is a highly specialized

228 centus are highly pinched at multiple sites (cell separation phenotype) and they do not divide to pro

229 ackground and causes slow-growth and delayed-cell-separation phenotypes in the S288C strain backgroun

230 onally designed microfluidic magnetophoretic cell separation platform capable of throughputs of 240 m

231 In this microfluidic cell separation platform, flexible cells with high defor

232 In cell separations, positive selection (capture of the tar

233 within analytical chemistry and proteomics, cell separations predominantly rely upon the second, lab

234 ) was applied to several clinically relevant cell separation problems, including the purging of human

235 oteins was found to be released while border cell separation proceeds.

236 IKE2 (HSL2), have been shown to activate the cell separation process that leads to organ abscission.

237 be selectively digested during the critical cell separation process.

238 ntional laboratory methods, showing that the cell separation product in the outlet reservoir was of m

239 he mother-bud neck, and septum formation and cell separation rapidly ensue.

240 cyte growth factor (HGF) induces endothelial cell separation, regulates expression of cell adhesion m

241 involved in pheromone response and daughter cell separation, respectively.

242 e transcriptase polymerase chain reaction of cell separations showed that the increased production of

243 The demonstrated cell separation shows promising applications of the DEP

244 nd hypothesize that CST negatively regulates cell separation signaling directly and indirectly.

245 We conclude that elements of the same cell separation signaling module have been adapted to fu

246 the orchestration of events during the final cell separation step of cell division called abscission.

247 region of Gag that is critical for the virus-cell separation step) is involved in controlling particl

248 In addition, cell separation studies and flow cytometric analyses rev

249 Cell separation studies showed that bright gp70(+) cells

250 Cell separation studies showed that the increased argina

251 Cell separation studies showed that the unresponsiveness

252 tunately, many current selection methods for cell separation, such as magnetic activated cell sorting

253 odeoxynucleotides prevented endothelial cell-cell separation, suggesting that Slug acts early in the

254 t was prepared using the Isolex 300 Magnetic Cell Separation System (Baxter Immunotherapy, Irvine, CA

255 ortant role in such a sedimentation-involved cell separation system.

256 oretic field-flow-fractionation (DEP-FFF), a cell-separation technique that exploits the differences

257 Utilizing cell separation techniques, it was determined that the w

258 Physical cell separation techniques, such as cell sorting and las

259 For analysis of rare cell separation, the local viscosity distribution is cal

260 Although Gef3p is not essential for cell separation, the simultaneous disruption of gef3(+)

261 , including two genes required for efficient cell separation: the chitinase-encoding gene CTS1 and th

262 cytokinesis, and also functions to maintain cell separation through much of the subsequent interphas

263 a wide range of applications including blood cell separation, ultrasound contrast agent preparation,

264 This quadruple mutant is delayed at cell separation upon release from mitotic arrest.

265 )-cross-linked pectic polymers and extensive cell separation upon tissue disruption.

266 Cell separation using dielectrophoresis as well as elect

267 iched preparations were isolated by magnetic cell separation using the FDC-restricted monoclonal anti

268 controls transcription of genes involved in cell separation, we show that disruption of some of thes

269 ces between individual cell types, efficient cell separations were achieved by dielectrophoresis on t

270 Cell separations were achieved in a thin chamber equippe

271 RNA-binding protein Scw1 and severely delays cell separation when combined either with a septin mutat

272 ease of fabrication and use is suitable for cell separations when subsequent analysis of target cell

273 TGFbeta2 mediates initial endothelial cell-cell separation while TGFbeta3 is required for the cell

274 art1 cells lyse during cell separation with a thinner and defective septum.

275 Thus, we propose that Cdc14 coordinates cell separation with mitotic exit via FEAR-initiated pho

276 circulating tumor cells, and for label-free cell separation with potential applications in biologica

277 Organ detachment requires cell separation within abscission zones (AZs).

278 tion of Neisseria gonorrhoeae ltgC inhibited cell separation without affecting peptidoglycan monomer

279 me atmospheric composition stimulated border cell separation without significantly influencing root g