ライフサイエンスコーパス: cell-free system

1 s the joining of complementary DNA ends in a cell free system.

2 n-can be recapitulated in vitro with a yeast cell-free system.

3 titis C virus IRES-mediated translation in a cell-free system.

4 ficantly decreased protein production in the cell-free system.

5 us type 1 (HIV-1) reverse transcriptase in a cell-free system.

6 These findings were partially mimicked in a cell-free system.

7 terial cloning because it amplifies DNA in a cell-free system.

8 2E1 (CYP2E1) and as purified components in a cell-free system.

9 rdiomyocyte culture after characterizing the cell-free system.

10 hout the biological reductant ascorbate in a cell-free system.

11 lso increased caspase-3 and -9 cleavage in a cell-free system.

12 acterized their deubiquitinase activity in a cell-free system.

13 ansfected and infected cell lysates and in a cell-free system.

14 n metabolism in HaCaT keratinocytes and in a cell-free system.

15 gation by forming an Abeta-heme complex in a cell-free system.

16 n-dUTP near the sites of its initiation in a cell-free system.

17 amino acid-fed and -deficient cells and in a cell-free system.

18 ent regulation of the V(D)J recombinase in a cell-free system.

19 o failed to alter HCV capsid assembly in the cell-free system.

20 capsid assembly in this highly reproducible cell-free system.

21 levels of infectivity can be generated in a cell-free system.

22 gocytophilum rapidly detoxifies O(2)(-) in a cell-free system.

23 a vacuole biogenesis was analyzed by using a cell-free system.

24 e than that of native alphaB-crystallin in a cell-free system.

25 g by apo-nNOS to form the active enzyme in a cell-free system.

26 istance for reporter mRNA translation in the cell-free system.

27 , which spontaneously hydrolyze in a defined cell-free system.

28 NAs could support protein translation in the cell-free system.

29 inhibitors of Tat-induced transcription in a cell-free system.

30 cy virus type 1 (HIV-1) capsid assembly in a cell-free system.

31 nstrate that this interaction can occur in a cell-free system.

32 in cells, as was observed previously in the cell-free system.

33 o were found to inhibit RNA synthesis in the cell-free system.

34 igand-dependent IL-10E1 phosphorylation in a cell-free system.

35 y, it completely blocks DNA end joining in a cell-free system.

36 ransduction is likely to be preserved in the cell-free system.

37 r co-receptor can be expressed in an E. coli cell-free system.

38 HMG2) also reproduce B12/23 restriction in a cell-free system.

39 ect can be reversed by addition of dATP in a cell-free system.

40 ciently than in the absence of survivin in a cell-free system.

41 inhibition of the cell cycle by MCM3AP in a cell-free system.

42 block holo-dependent caspase activation in a cell-free system.

43 ctly with aSyn in both intact cells and in a cell-free system.

44 shown to degrade glutathione in yeast and a cell-free system.

45 ngle-strand deoxyribonucleic acid (DNA) in a cell-free system.

46 mpared to fresh garlic extract in vitro in a cell-free system.

47 was heterologously expressed in a eukaryotic cell-free system.

48 ibit EGFR or other related receptor TKs in a cell-free system.

49 peroxide generation or elastase release in a cell-free system.

50 hol), whereas dA adducts predominated in the cell-free system.

51 anel of 25 pre-selected protein kinases in a cell-free system.

52 ferred pathway of ICL repair in a vertebrate cell-free system.

53 OL-dependent ubiquitination of the LDLR in a cell-free system.

54 to cognate precursor peptide in cellular and cell free systems.

55 ential advantages for protein elaboration in cell free systems.

56 ds (>mg ml(-1)) of proteins from the present cell-free systems.

57 ivity both in pancreatic cancer cells and in cell-free systems.

58 we have analyzed its interaction with Bax in cell-free systems.

59 mic instability on microtubules assembled in cell-free systems.

60 ompetitive affinity to TfR evaluated in cell/cell-free systems.

61 d to assess DNA binding and transcription in cell-free systems.

62 0 are targeted by p300HAT for acetylation in cell-free systems.

63 e monitored PHD activity both in vivo and in cell-free systems.

64 shock protein A and apomyoglobin (apoMb) in cell-free systems.

65 available to characterize target proteins in cell-free systems.

66 tic activity, and processing were defined in cell-free systems.

67 receptor-ligand interaction in cells and in cell-free systems.

68 2 and integrin alpha5beta1 was determined in cell-free systems.

69 interfered with the formation of hemozoin in cell-free systems.

70 ing cytoplasmic dynein that can be formed in cell-free systems.

71 alkylates and interstrand crosslinks DNA in cell-free systems.

72 synthesis in both prokaryotic and eukaryotic cell-free systems.

73 ound directly to integrin alpha(v)beta(3) in cell-free systems.

74 S936, a mechanism-based inhibitor of NQO1 in cell-free systems.

75 ns similar to those needed for DNA damage in cell-free systems.

76 kinase activity to Elk1 in both cellular and cell-free systems.

77 ions inside Chlamydia-infected cells than in cell-free systems.

78 examine directly protein-RNA interactions in cell-free systems.

79 ke GPCRs by coupling them to ion channels in cell-free systems.

80 ity were accurately reproduced in Tg mice or cell-free systems.

81 tion by Mdm2-MdmX E3 complex in cells and in cell-free systems.

82 tory elements using Escherichia coli extract cell-free systems.

83 ctase 2 (NQO2) for use either in cellular or cell-free systems.

84 and are equally attractive as reporters for cell-free systems.

85 escribed HIV-1 assembly intermediates in the cell-free system (10S, 80-150S, and 500S).

86 In a cell-free system, addition of actin to in vitro-generate

87 degraded by the 26S proteasomal pathway in a cell-free system, albeit not in an S-allele-specific man

88 In cell-free systems, Alix directly interacts with F-actin

89 Importantly, we show that the cell-free system allows for the rapid (2 h) identificati

90 Additionally, the use of cell-free systems allows the selection of proteins that

91 The cell-free system also replicated the full-length TBSV ge

92 Caspase-3 activates AN34 in a cell-free system, although caspase-3 cannot cleave Ape1

93 aracterization of this antibody using both a cell-free system and a cell model system of apoptosis de

94 ract with AIF either in intact cells or in a cell-free system and furthermore, failed to prevent nucl

95 tes post-translationally with HIV-1 Gag in a cell-free system and human T cells infected with HIV-1.

96 We found that, both in a cell-free system and in cells, NO/SNO donors such as S-n

97 ts suggest that NDV F protein made both in a cell-free system and in Cos-7 cells may exist in two top

98 t YY1 physically interacts with AR both in a cell-free system and in cultured cells.

99 s spectrometry and mutagenesis analyses in a cell-free system and in gliomas cells identified Tyr-7 a

100 interaction and for glycogen synthesis in a cell-free system and in intact cells.

101 potently reduces Abeta production in the N2a cell-free system and in intact N2a cells.

102 element AU-rich element 1 of bcl-2 mRNA in a cell-free system and in MCF-7 cells.

103 8 residue and associates with Ubc9 both in a cell-free system and in virus-infected BCBL-1 cells.

104 ditis virus (EMCV) and poliovirus IRESs in a cell-free system and in virus-infected HeLa cells.

105 cts with integrin alpha(v)beta(5), both in a cell-free system and on the cell surface of rat lung fib

106 Inhibition of the trypanosome cell-free system and recombinant rat GlcNAc-PI de-N-acet

107 SNAP also blocked caspase 9 activation in a cell-free system and reversibly inhibited catalytic acti

108 periments are consistent with those from the cell-free system and strongly suggest that ara-G is phos

109 at HIV-2 Gag associates with human HP68 in a cell-free system and that Gag proteins of HIV-2, simian

110 fects of these mutations on RNA binding in a cell-free system and TTP-induced mRNA instability in cel

111 e mechanisms governing EB/tau interaction in cell-free systems and cellular models.

112 Using cell-free systems and different cellular models, we show

113 e of the critical residues identified in the cell-free systems and exploring the limit of CPAF's tole

114 ther biological networks behave similarly in cell-free systems and in cells.

115 CP is essential for actin-based motility in cell-free systems and in Dictyostelium.

116 ge activity toward its effector Rap1 both in cell-free systems and in intact cells.

117 domain Bax or Bak, or BH3-only Bim or Bad in cell-free systems and in intact human cancer cells, free

118 y co-immunoprecipitation studies, using both cell-free systems and mammalian cells, and the specific

119 Binding analyses in cell-free systems and on the cell membrane demonstrate t

120 bition of NQO2 activity was assessed in both cell-free systems and the human leukemia K562 cell line.

121 roplatelet formation can be recapitulated in cell-free systems and their biochemistry evaluated; the

122 (TcdB) has been studied extensively by using cell-free systems and tissue culture, but, like many bac

123 We used cell-free systems and ultimately a vesicular reconstitut

124 M2 promotes the ubiquitination of FOXO1 in a cell-free system, and its knockdown by small interfering

125 n from HSP2 has been reported only once in a cell-free system, and never when recombinant proteins ha

126 e demonstrated both in intact cells and in a cell-free system, and proteasome inhibition or Huwe1 sil

127 stranded RNA-dependent protein kinase PKR in cell-free systems, and within latently infected B-cell l

128 Capsids produced in cell-free systems are also indistinguishable from capsid

129 Cell-free systems are appealing alternative environments

130 olesterol (FC) bilayer membranes in cell and cell-free systems are compared.

131 Commercially available cell-free systems are freeze dried onto paper, enabling

132 Ro106-9920 was identified in a cell-free system as a time-dependent inhibitor of Ikappa

133 The GSMs modulate Abeta both in cell and cell-free systems as well as lower amyloidogenic Abeta42

134 In this cell-free system, as in mammalian cells, capsid assembly

135 activation by recruitment in a reconstituted cell-free system, assembled entirely from a defined numb

136 In a cell-free system, ATP hydrolysis by the v-ATPase was nec

137 l tombusvirus of plants, we have developed a cell-free system based on a Saccharomyces cerevisiae ext

138 c reticulum (ER) has been reconstituted in a cell-free system based on interphase Xenopus egg extract

139 In a cell-free system based on the immunoglobulin heavy chain

140 Here, we describe a cell-free system based on Xenopus egg extracts that supp

141 is known to mediate actin/MT interactions in cell-free systems but the role of tau in regulating cyto

142 vitro-translated MCL-1 can be degraded in a cell-free system by the 20S proteasome.

143 n vitro method to generate DNA templates for cell-free systems, bypassing the need for DNA template g

144 alternate forward engineering paradigm using cell-free systems can thus accurately capture cellular b

145 We have developed a cell-free system capable of processing and joining nonco

146 alytically less efficient than intact PE3 in cell-free systems, co-expression in live cells transfect

147 We have established a cell-free system competent for replication in which all

148 cate cell-bound system and (ii) a simplistic cell-free system composed of a single cohesin-containing

149 ated kinase 2 (PAK-2) was recapitulated in a cell-free system consisting of in vitro-transcribed RNA,

150 import of phytochromes can be analyzed in a cell-free system consisting of isolated nuclei of the un

151 ause direct inhibition of PARP activity in a cell-free system containing PARP and NAD(+) but did coun

152 % of HCV core protein synthesized de novo in cell-free systems containing rabbit reticulocyte lysate

153 tly, we demonstrate that characterization in cell-free systems correlates and is reflective of perfor

154 In a cell-free system, CPAF activity is both necessary and su

155 Second, addition of wortmannin to the cell-free system demonstrated that Nef activation of PAK

156 Experiments in both breast cancer cells and cell-free systems demonstrated that niclosamide possesse

157 A cell-free system depleted of intersectin failed to suppo

158 this question by imaging growing asters in a cell-free system derived from eggs, where asters grew to

159 We previously used a soluble cell-free system derived from Xenopus eggs to investigat

160 Cell-free systems designed to perform complex chemical c

161 y to proteasome-dependent degradation in the cell-free system did not increase.

162 However, increasing amounts of Alpha2 in a cell-free system disrupted the formation of Dab1-Lis1 co

163 elieve that these data are unattainable in a cell-free system due to the poor replication of these CR

164 Our unique cell-free system enables complete control and manipulati

165 r additional cloning steps, which makes this cell-free system fast and efficient.

166 Nucleotides 6-9 inhibited oxidation in cell-free systems (Fe(II)-H2O2), as detected by ESR (IC5

167 s study also illustrates the utility of this cell-free system for investigating hypotheses of recepto

168 contribute to mRNA turnover, we developed a cell-free system for mRNA turnover using the trypanosome

169 This protocol describes a cell-free system for studying vertebrate centromere and

170 These membrane fractions were used in a cell-free system for the analysis of HCV RNA replication

171 We established a cell-free system from individual Drosophila melanogaster

172 By the use of a cell-free system from Xenopus eggs that reproduces the m

173 emonstration of an ion channel in eukaryotic cell-free system has a large potential for future applic

174 In summary, the cell-free system has defined several RNA turnover steps

175 t progress in propagating TSE infectivity in cell-free systems has effectively ruled out the involvem

176 th both intact cells and a kinase-dependent, cell-free system have suggested that protein kinase C ca

177 Experiments in cell-free systems have demonstrated that the VP5 cleavag

178 tively blocked gamma-secretase activity in a cell-free system (IC50 = 30 nM).

179 T bundles and 2D MT bundles may assemble, in cell free systems in the presence of counter-ions, revea

180 capsid assembly and RNA encapsidation in the cell-free system in a manner similar to that seen in mam

181 ailed to directly inhibit Cdc2 activity in a cell-free system in spite of direct association between

182 tivation of the oxidase in a semirecombinant cell-free system in the absence of an amphiphilic activa

183 An analysis of GPIs formed in a cell-free system in the presence and absence of glucosam

184 Using a cell-free system in vitro, we evaluated the action of DM

185 spase-3 and -8 in liver homogenates and in a cell-free system in vitro.

186 We have developed a mammalian cell-free system in which HBc is expressed at physiologi

187 We have established a cell-free system in which mitochondria derived from viru

188 Recently, we established a cell-free system in which over 60% of full-length HCV co

189 action between two molecular systems using a cell-free system in which polystyrene microspheres funct

190 mechanism of apoB degradation, we employed a cell-free system in which proteasome-dependent degradati

191 We developed a cell-free system in which TTP and its related proteins s

192 RacC stimulates F-actin assembly in cell-free systems in a WASP-dependent manner.

193 f the time-course of protein expression in a cell-free system, in conjunction with the development of

194 Using a cell-free system, in vitro-translated 35S-labeled KLF4 p

195 The addition of purified RatA to a cell-free system inhibited the formation of 70S ribosome

196 addition of phosphorylated p40(PHOX) to the cell-free system inhibits NADPH oxidase activated by pro

197 Studies were performed in a cell-free system involving endothelial PM sheets and iso

198 As demonstrated herein, the cell-free system is a new and important tool in the inve

199 amma-secretase generation of Abeta in an N2a cell-free system is ATP dependent.

200 single-protein production can be achieved in cell-free systems, it is not easy to completely suppress

201 A replication in a Xenopus egg extract based cell-free system, leading to the expectation that the pr

202 This cell-free system lends itself to use in protein immunode

203 lar requirements for capsid formation, these cell-free systems make a mechanistic dissection of HCV c

204 In a cell-free system, Mediator directly and substantially in

205 stabilization of oligomeric intermediates in cell-free systems, no studies have examined the effects

206 In cell-free systems, Nrdp1 has ubiquitin ligase (E3) activ

207 In a cell-free system, obatoclax induced an activating confor

208 Furthermore, in a cell-free system of IKK complex activation by TRAF6 (TNF

209 Cell-free systems offer several advantages over traditio

210 ence on translational efficiency in either a cell-free system or cell culture, indicating that any AG

211 n adaptors, whereas functional studies using cell-free systems or intact cells have demonstrated the

212 Interaction with soluble CAR in a cell-free system, or with CAR on the surfaces of transfe

213 metry of the nuclear transport factor 2 in a cell-free system over a broad concentration range.

214 In cell-free systems, p53R2 did not oxidize a reactive oxyg

215 tochrome c-induced caspase-3 activation in a cell-free system, particularly in the presence of H(2)O(

216 lcytosine (used as a substrate for dCK) in a cell-free system; phosphorylation of this compound by dG

217 In cell-free systems, polyamides have been shown to regulat

218 s were able to inhibit RNA synthesis in this cell-free system, presumably through chain termination,

219 ng ability on par with other flavonoids in a cell-free system, Proxison is orders of magnitude more p

220 purified Pyk2 can be activated by acid in a cell-free system, Pyk2 may serve as the pH sensor that i

221 In a cell-free system, recombinant PKC-zeta phosphorylates LK

222 In cell-free systems, recombinant IRE1alpha endonucleolytic

223 at stabilizes microtubules in neurons and in cell-free systems regulates actin-microtubule interactio

224 Cell-free systems represent a promising approach to quic

225 at autophosphorylation of Chk2 produced in a cell-free system requires trans phosphorylation by a wor

226 transfer of heavy chains to hyaluronan in a cell-free system, restore the expansion of Tnfip6-null c

227 Oscillation periods in cells matched the cell-free system results for all networks tested.

228 Additionally, in a cell-free system, resveratrol directly induced the depol

229 However, examination of apoptosis in a cell-free system revealed a block in chromatin condensat

230 On the basis of studies using cell-free systems, ribosomal frameshifting can explain t

231 The cell-free system should now allow for the definition of

232 Altogether, tombusvirus replicase in the cell-free system showed features remarkably similar to t

233 The cell-free system showed high template specificity, since

234 Yet cell-free systems showed that recognition was mediated o

235 failed to induce PPARdelta binding to DRE in cell-free system, suggesting that cPLA2alpha-mediated AA

236 omolar concentration in both intact-cell and cell-free systems, suggesting that these inhibitors targ

237 ADPH oxidase regulation using whole cell and cell-free systems suggests that the toxins do not exert

238 animal myocytes, E. coli, and the wheat germ cell-free system than Mbs from terrestrial mammals.

239 hat generates active PS, we used a mammalian cell-free system that allows de novo human PS NTF and CT

240 nts of PCD, we have developed an Arabidopsis cell-free system that can be used to monitor biochemical

241 We also confirmed in a cell-free system that ER-alpha is an inhibitory regulato

242 ecular level, we have recently established a cell-free system that initiates chromosomal DNA replicat

243 In addition, using a cell-free system that makes both G and SG RNA, we show t

244 In this paper, using a cell-free system that recapitulates end synapsis and DNA

245 erstand these processes, we have developed a cell-free system that recapitulates these early steps of

246 Here we present a cell-free system that reconstitutes fragmentation of pur

247 Using a cell-free system that synthesizes both SG and G RNA, we

248 mES Ex provide a novel cell-free system that uses the immense regenerative powe

249 e it has been predicted from some studies of cell-free systems that mutations may occur with a freque

250 the agent, the prion, can be replicated in a cell-free system, that it can be generated de novo, and

251 We characterized in a cell-free system the 'repressilator', a three-node synth

252 ous studies, however, we demonstrate that in cell-free systems the mode of action of selected NSAIDs

253 Because these studies are primarily based on cell-free systems, the role of the ubiquitin ligase acti

254 Here we describe the use of a cell-free system to characterize the effect of T Ag on p

255 ole in this process, we used a reconstituted cell-free system to define the precise contribution of p

256 ramide's inhibitory effect on PLD, we used a cell-free system to examine PLD activity and translocati

257 In this study, we have used a cell-free system to examine the requirements for microtu

258 Here, we used an Escherichia coli-based cell-free system to express a MOMP protein from the mous

259 Using a cell-free system to initiate replication within G1-phase

260 Here we use a reconstituted cell-free system to investigate the mechanism and extent

261 We previously employed a cell-free system to investigate the nature of the vesicl

262 We developed a cell-free system to recapitulate cytokinesis signaling u

263 mbly during replication and provide a facile cell-free system to study capsid assembly under physiolo

264 ning and plasmid transport, we established a cell-free system to study plasmid partition reactions in

265 ility to function as reporters in completely cell-free systems to allow for the extremely rapid and s

266 5-bisphosphate (PIP(2)) levels in intact and cell-free systems to provide electrophysiological and bi

267 e surfactants in commercial Escherichia coli cell-free systems to rapidly produce milligram quantitie

268 Using this cell-free system together with a variety of pharmacologi

269 protein was monitored in an Escherichia coli cell-free system under different experimental conditions

270 We show here in a cell-free system using incorporation of radioactive guan

271 studied the lipin 1beta enzyme activity in a cell-free system using PA/Triton X-100 mixed micelles as

272 of regulated recombinase accessibility in a cell-free system using plasmid substrates assembled into

273 ed the interface of the Bcl-2 homodimer in a cell-free system using site-specific photocross-linking.

274 n of TR1 by the IQs was studied in detail in cell-free systems using purified enzyme.

275 een characterized, either in living cells or cell-free systems, using radioactive compounds for quant

276 ic ability of radiomimetics to cleave DNA in cell-free systems, varying in activity from 2-fold (desc

277 A cell free system was developed, and a succession of comp

278 ated by immunoprecipitation and studied in a cell-free system was activated and phosphorylated at aci

279 fully replicating the TBSV replicon RNA, the cell-free system was also capable of generating TBSV RNA

280 The cell-free system was capable of performing a complete re

281 The cell-free system was used to analyze the mechanism of Ne

282 Using a cell-free system we show that hypersensitivity does not

283 Using in vitro cell free systems, we demonstrated that apratoxin A prev

284 Here, using a cell-free system, we demonstrate that although recombina

285 depletion-reconstitution experiments in the cell-free system, we demonstrate that HP68 is essential

286 With a cell-free system, we demonstrated the conversion of meth

287 Using a cell-free system, we have directly and systematically in

288 Using a cell-free system, we recently identified the phosphoinos

289 Using a cell-free system, we showed that FANCI-FANCD2 is require

290 Using these cell-free systems, we show that HCV capsid assembly is i

291 parum proteins prepared using the wheat germ cell-free system (WGCFS).

292 le, inhibits protein synthesis in an E. coli cell-free system, whereas the addition of PemI, the anti

293 factors or conditions not yet reproduced in cell-free systems which contribute to fidelity during re

294 In a cell-free system with externally added DHA, nearly 70% o

295 We used a cell-free system with pure Escherichia coli components t

296 In a cell-free system with purified transport factors alpha-s

297 PKR autophosphorylation in a cell-free system with recombinant NLRP3, apoptosis-assoc

298 ng properties of eight TCR/pMHC couples in a cell-free system with single bond resolution.

299 on (O2.-) production in a reconstituted Nox1 cell-free system, with no effect on Nox2-, Nox4-, Nox5-,

300 y, VapC-mt4 inhibited protein synthesis in a cell-free system without cleaving the corresponding mRNA