ライフサイエンスコーパス: cellubrevin

1 of maturation into phagolysosomes, acquired cellubrevin.

2 y of the syb mutations, as can rat VAMP2 and cellubrevin.

3 endosomes, BAP31 did not cofractionate with cellubrevin.

4 In this work, we report that cellubrevin, a v-SNARE functioning in endosomal recyclin

5 of the cytoplasmic domains of VAMP2 or VAMP3/cellubrevin also resulted in an inhibition of insulin-st

6 The observed cellubrevin alteration on mycobacterial phagosomes was n

7 These data suggest that cellubrevin and AP-1B cooperate in basolateral membrane

8 y colocalized with transferrin receptors and cellubrevin and morphologically resembled the recycling

9 The ESVs contain cellubrevin and Rab4 but are lacking other early endosom

10 -deficient neurons but that deletion of both cellubrevin and synaptobrevin does not cause a more seve

11 eimide attachment protein receptors (SNAREs) cellubrevin and vesicle-associated membrane protein-2 (V

12 ed with recycling endosomes, such as rab 11, cellubrevin, and endobrevin, but relatively poor in earl

13 rane protein (VAMP-2), its related homologue cellubrevin, and syntaxin-4.

14 ment (ERC), which is marked by Rab11a, VAMP3/cellubrevin, and VAMP8/endobrevin and holds large reserv

15 lysin O-permeabilized platelets to antihuman cellubrevin antibody inhibited Ca(++)-induced alpha-gran

16 We report that cellubrevin binds with high specificity to BAP31, a repr

17 e to the wild-type TR, and sphingomyelin and cellubrevin, both of which label all compartments of the

18 ytoplasmic tail of BAP31 prevented export of cellubrevin, but not of the transferrin receptor from th

19 unocytochemistry, BAP31 did not overlap with cellubrevin, but rather colocalized with resident protei

20 nstrate that syntaxin 4, VAMP2, and/or VAMP3/cellubrevin can function as target membrane and vesicle

21 onally involved in receptor recycling [Rab4, cellubrevin (Cbvn)] relative to the distribution of a re

22 Furthermore, we demonstrate that cellubrevin coimmunoprecipitates preferentially with syn

23 blocking phagosomal maturation did not cause cellubrevin degradation on model phagosomes.

24 rified with BAP31 on immobilized recombinant cellubrevin, demonstrating that the interaction is speci

25 In COS cells, cellubrevin endosomes (mean pHCb 6.1 +/- 0.05; range, 5.

26 internalized FITC-F(ab) to measure the pH of cellubrevin-enriched recycling endosomes (pHCb) and FITC

27 ociated membrane protein (VAMP) 1, 2, or rat cellubrevin failed to detect these v-SNAREs in human pla

28 Termed human cellubrevin (Hceb), this protein has greater than 93% id

29 y internalized by COS cells transfected with cellubrevin-Ig, which at steady state accumulated in a p

30 demonstrated that approximately 80% of human cellubrevin in resting platelets was localized to platel

31 oxin D, which selectively cleaves VAMP-2 and cellubrevin, inhibited the ability of insulin to stimula

32 Cellubrevin is a ubiquitously expressed membrane protein

33 The interaction between BAP31 and cellubrevin is sensitive to high ionic strength and appe

34 rescent protein, that CFTR affects the pH of cellubrevin-labeled endosomal organelles resulting in hy

35 show that the ubiquitously expressed v-SNARE cellubrevin localizes to the basolateral membrane and to

36 n 93% identity with human VAMP 1, 2, and rat cellubrevin over the conserved core region, but has a un

37 hree mammalian isoforms, VAMP-1, VAMP-2, and cellubrevin, play a role in protein transport to the pla

38 These results suggest that cellubrevin plays a role in phagosomal maturation and th

39 how that the closely related R-SNARE protein cellubrevin rescues synaptic transmission in synaptobrev

40 f 3 functional platelet SNARE proteins-human cellubrevin, SNAP-23, and syntaxin 2.

41 Cellubrevin status on phagosomes had consequences on pha

42 In contrast, cellubrevin, VAMP-2, and syntaxin 4 protein levels were

43 th rosiglitazone (30 micromol/kg) normalized cellubrevin, VAMP-2, and syntaxin 4 protein to levels ap

44 Cellubrevin/VAMP-3 has been proposed to be a critical v-

45 us, and null platelets confirmed the lack of cellubrevin/VAMP-3 in nulls and showed that most element

46 These data show that the v-SNARE, cellubrevin/VAMP-3 is not a requirement for the platelet

47 exocytosis, we have analyzed platelets from cellubrevin/VAMP-3 knockout mice.

48 mbrane Protein (VAMPs: synaptobrevin/VAMP-2, cellubrevin/VAMP-3, TI-VAMP/VAMP-7, and endobrevin/VAMP-

49 Although several SNAREs, including VAMP3/cellubrevin, VAMP8/endobrevin, syntaxin 13, and syntaxin

50 attachment protein receptor (v-SNARE) called cellubrevin/vesicle-associated membrane protein-3 (VAMP-

51 , one in which the vesicle transport protein cellubrevin was appended with a luminal IgG epitope to a

52 Furthermore, a fraction of BAP31 and cellubrevin was complexed when each of them was quantita

53 tion of alpha-granule secretion by antihuman cellubrevin was reversed by a blocking peptide.

54 Cleavage of cellubrevin with tetanus neurotoxin (TeNT) results in sc