ライフサイエンスコーパス: cellulosome

1 those of the larger, multienzyme complexes (cellulosomes).

2 bility, and higher order organization of the cellulosome.

3 rs to dissociate the oligomeric state of the cellulosome.

4 s a key role in the Clostridium thermocellum cellulosome.

5 protein scaffold forming a complex called a cellulosome.

6 atus of anaerobic microorganisms, termed the cellulosome.

7 r biotechnological development of the fungal cellulosome.

8 g apparatus of anaerobic bacteria termed the cellulosome.

9 icellular eukaryotes, also reside within the cellulosome.

10 tive enzymes were known to reside within the cellulosome.

11 nzyme cellulose-degrading complex called the cellulosome.

12 n enzymatic subunits of the C. cellulovorans cellulosome.

13 ng for CelK, a 98-kDa major component of the cellulosome.

14 n extracellular cellulase complex termed the cellulosome.

15 me and properly integrated into the designer cellulosome.

16 aratus into a large multienzyme complex, the cellulosome.

17 's most elaborate multienzyme complexes, the cellulosome.

18 processes exemplified by the assembly of the cellulosome.

19 f beta-glucosidase would be available to the cellulosome.

20 ay enable new strategies to create designers cellulosomes.

21 ing ways to improve biomass conversion using cellulosomes.

22 -grained model to study the self-assembly of cellulosomes.

23 cell surface and complements the activity of cellulosomes.

24 or role and a minor role in C. cellulovorans cellulosomes.

25 ins was suggested as a model for assembly of cellulosomes.

26 cellulose hydrolysis that are distinct from cellulosomes.

27 t when compared to this pattern of untreated cellulosomes.

28 bled into large multienzyme complexes termed cellulosomes.

29 nt synergies with pivotal cellulases in mini-cellulosomes.

30 syl hydrolases into large complexes known as cellulosomes.

31 in type I dockerins that direct enzymes into cellulosomes.

32 ce for the various catalytic subunits of the cellulosome, a large extracellular cellulase complex.

33 idium thermocellum produces the prototypical cellulosome, a large multienzyme complex that efficientl

34 ciated glycosyl hydrolase into the bacterial cellulosome, a multienzyme cellulolytic complex, via its

35 lulosic biomass either by free enzymes or by cellulosomes, ability to rapidly ferment hexose and pent

36 recruit hemicellulases to the sophisticated cellulosomes acting in the gastrointestinal tract of mam

37 In this study, we complemented a cellulosome active on cellulose and hemicellulose by add

38 acts will provide more detailed insight into cellulosome activity.

39 The dynamic integration of enzymes in cellulosomes, afforded by the dual binding mode, is beli

40 retreated switchgrass compared to the native cellulosome alone or in combination with wild-type BglA

41 LPMOs were able to self-assemble in designer cellulosomes alongside an endo- and an exo-cellulase als

42 Consequently, a model of the entire cellulosome and a detailed picture of intermodular conta

43 Complexation of the cellulosome and BglA-CohII resulted in higher overall de

44 EngF is not a component of the cellulosome and does not contain a duplicated sequence (

45 e CipA, which is a structural protein of the cellulosome and has a strong affinity for calcium.

46 Comparison of cellulosome and noncellulosomal enzymes showed that expr

47 Ca is incorporated into the cellulosome and tightly bound as demonstrated using 45Ca

48 p, changes in mRNA and protein expression in cellulosomes and noncellulosomal (hemi)cellulolytic enzy

49 Cellulosomes and noncellulosomal (hemi)cellulolytic enzy

50 lable carbon source and that synergy between cellulosomes and noncellulosomal enzymes contribute to p

51 Cellulosomes and noncellulosomal enzymes had synergistic

52 scription and protein analyses revealed that cellulosomes and noncellulosomal enzymes were expressed

53 dockerin modules, the basic elements of the cellulosome, and manual sequencing of partially sequence

54 lulosomal protease inhibitors would regulate cellulosome architecture and then lignocellulose hydroly

55 The basic elements of its cellulosome architecture, mainly cohesin and dockerin mo

56 ducing bacteria, but reveal a more organized cellulosome architecture.

57 lusters comprising fundamental components of cellulosome architecture.

58 from the proximity effects generated by the cellulosome architecture.

59 ng profiles which implied two major types of cellulosome architectures: (i) an intricate cell-bound s

60 Since gene clusters for the cellulosome are also found in C. cellulolyticum and C. j

61 Cellulosomes are assembled by selective high-affinity bi

62 Cellulosomes are key for lignocellulosic biomass degrada

63 Cellulosomes are large, multienzyme, plant cell wall-deg

64 Cellulosomes are large, multiprotein complexes that teth

65 Cellulosomes are multienzyme complexes responsible for e

66 Cellulosomes are polyprotein machineries that efficientl

67 Cellulosomes are produced exclusively by anaerobic micro

68 ABTRACT: Cellulosomes are sophisticated multi-enzymatic nanomachi

69 Cellulosomes are typically recruited to the cell surface

70 XynY and XynZ, components of the cellulosome, are composed of several domains including x

71 nd to be a component of the C. cellulovorans cellulosome as well as the previously reported endogluca

72 The Clostridium thermocellum cellulosome assembles through the interaction of a type

73 ockerin-bearing ORFs potentially involved in cellulosome assembly and a variety of multi-modular scaf

74 Thus, this work reveals a novel mechanism of cellulosome assembly and challenges the ubiquitous impli

75 w dockerin-cohesin interactions mediate both cellulosome assembly and the retention of the protein co

76 Cellulosome assembly involves the binding of enzyme-born

77 racterization of the factors influencing the cellulosome assembly process may enable new strategies t

78 rehensive set of proteins critical to fungal cellulosome assembly, including conserved scaffolding pr

79 In the canonical model of cellulosome assembly, type I dockerin modules of the enz

80 e contains a dockerin domain responsible for cellulosome assembly.

81 ues, is proposed, providing new insight into cellulosome assembly.

82 increased conformational flexibility during cellulosome assembly.

83 imary scaffoldin, and type I modules mediate cellulosome attachment to an anchoring scaffoldin.

84 obal exploration of PTMs associated with the cellulosome based upon a set of defined PTMs that includ

85 to the surface of C. thermocellum and not to cellulosomes because it binds preferentially to type I c

86 me will be useful for rumen microbiology and cellulosome biology and in biofuel production, as one of

87 e to the integration of enzymes into primary cellulosomes but can also characterize polycellulosome a

88 rin module and were formerly detected in the cellulosomes, but only three of them were previously stu

89 t the intriguing possibility that individual cellulosomes can associate with one another via inter-sc

90 eviously, a dual-binding mode is involved in cellulosome cell-surface attachment, whereas single-bind

91 ar mechanisms used by anaerobic bacteria for cellulosome cellular attachment.

92 rporation of all GH9 cellulases in trivalent cellulosome chimera containing Cel48F and Cel9G generate

93 ern was investigated in the free state or in cellulosome chimeras with key cellulosomal cellulases.

94 of binding both to CbpA to form a CbpA-EngE cellulosome complex and to the surface layer of C. cellu

95 iens produces a highly organized multienzyme cellulosome complex that plays a key role in the degrada

96 ellulose degradation activity by binding the cellulosome complex to the cellulose substrate; secondar

97 arrangement of the enzymatic subunits in the cellulosome complex, made possible by the scaffoldin sub

98 izes cellulose with the aid of a multienzyme cellulosome complex.

99 rocedures did not involve a clostridium-like cellulosome complex.

100 ed by assembly of the intricate multi-enzyme cellulosome complex.

101 and was readily incorporated into the native cellulosome complex.

102 d to the cell surface and involves elaborate cellulosome complexes in specialized cellulolytic specie

103 e of HLDs on cellulose degradation, the mini-cellulosome complexes with HLDs degraded cellulose more

104 crucial role in the assembly of multienzyme cellulosome complexes.

105 ression of xylanase activity and to vary the cellulosome composition depending on the growth substrat

106 on for future studies of biomass conversion, cellulosome composition, and clostridial systems biology

107 rio cellulolyticus produces a highly complex cellulosome comprising an unusual adaptor scaffoldin, Sc

108 yed pivotal roles in describing the original cellulosome concept.

109 the importance of GH9 diversity in bacterial cellulosomes, confirm that Cel9G is the most synergistic

110 complex of Clostridium thermocellum, termed cellulosome, consists of up to 26 polypeptides, of which

111 e degradation by three different recombinant cellulosomes containing either endoglucanase EngE, endog

112 The mixture of the cellulosomes containing ExgS and EngH showed higher acti

113 ion of Clostridium cellulovorans recombinant cellulosomes containing the enzymatic subunit EngB and t

114 n addition to a large collection of integral cellulosome-containing proteins.

115 caffoldin and what governs the population of cellulosomes created during self-assembly.

116 e gel electrophoresis analysis revealed that cellulosomes derived from xylan-, cellobiose-, and cellu

117 Therefore, in contrast to all cellulosomes described to date, the assembly of R. flave

118 species sequenced fall within the class III cellulosome-encoding Clostridium and the class V sacchar

119 it was found that the native C. thermocellum cellulosome exists as a homooligomer and the high-affini

120 ioinformatic analyses of the R. flavefaciens cellulosome failed to identify a CBM predicted to bind t

121 the dual-binding mode in the acquisition of cellulosome flexibility.

122 Several mechanisms involved in cellulosome formation remain unknown, including how cell

123 lot analysis was carried out with the native cellulosome fraction bound to cell wall fragments, the p

124 Purified cellulosomes from C. thermocellum were found to hydrolyz

125 Also, when xylanase activity in the cellulosomes from the xylan-grown cultures was compared

126 ngB, one component of the cellulase complex (cellulosome) from Clostridium cellulovorans, by the dire

127 n the catalytic subunits is a key feature of cellulosome function.

128 A comparison of the glycoside hydrolase and cellulosome functional genes revealed that in the rumen

129 ne cluster for the Clostridium cellulovorans cellulosome has been cloned and sequenced upstream and d

130 or subunits of the Clostridium cellulovorans cellulosome, has been cloned and sequenced.

131 or subunits of the Clostridium cellulovorans cellulosome, has been determined.

132 or subunits of the Clostridium cellulovorans cellulosome, has been isolated and sequenced.

133 Recently, designer cellulosomes have been developed to incorporate foreign

134 The intact cellulosomes hydrolyzes crystalline cellulose in the pre

135 nism for covalent cell-wall anchoring of the cellulosome in R. flavefaciens differs from those report

136 assembly and cell surface attachment of the cellulosome in R. flavefaciens, we determined the crysta

137 ions were also performed by adding different cellulosomes in a sequential manner.

138 sible for the structural organization of the cellulosome, including olpB, orf2p, and olpA.

139 reas single-binding interactions operate for cellulosome integration of enzymes.

140 bed to date, the assembly of R. flavefaciens cellulosome involves single but not dual-binding mode Do

141 The cellulosome is a multiprotein complex, produced primaril

142 The core of the cellulosome is a noncatalytic scaffoldin protein, which

143 nzyme beta-glucosidase; however, because the cellulosome is adsorbed to the insoluble substrate only

144 These findings suggest that the fungal cellulosome is an evolutionarily chimaeric structure-an

145 Ruminococcus flavefaciens' cellulosome is assembled from a repertoire of 223 Doc-co

146 responsible for the enzymatic synergy of the cellulosome is hindered by the large size and inherent f

147 A central feature of bacterial cellulosomes is a large non-catalytic protein, the scaff

148 he biocatalytic activity of anaerobic fungal cellulosomes is expanded by the inclusion of GH3, GH6 an

149 ent dockerins to assemble the most intricate cellulosome known to date.

150 y identified the physiological importance of cellulosome-localized protease inhibitors in Clostridia.

151 northodox use of aerobic enzymes in designer cellulosome machinery effects simultaneous degradation o

152 exibility in the quaternary structure of the cellulosome may reflect the challenges presented by the

153 activity and synergy degrees than the other cellulosome mixtures, indicating the synergistic effect

154 This sui generis cellulosome model enhances our understanding of the mech

155 es of the solventogenesis pathway and of the cellulosome of C. acetobutylicum comprise a new set of m

156 The cellulosome of Clostridium cellulovorans consists of thr

157 The cellulosome of Clostridium thermocellum is a multipolype

158 The cellulosome of Clostridium thermocellum is a multiprotei

159 The cellulosome of Clostridium thermocellum JW20 consists of

160 t the extended repertoire of proteins in the cellulosome of R. flavefaciens contributes to an extende

161 During the course of evolution, the cellulosome, one of Nature's most intricate multi-enzyme

162 s play a pivotal role in the assembly of the cellulosome, one of nature's most intricate nanomachines

163 ell envelope proteins, thereby anchoring the cellulosome onto the bacterial cell.

164 This report reveals that recruitment of cellulosomes onto the cell surface may involve dockerins

165 al for the in vitro assembly of a "designer" cellulosome, or a recombinant cellulosome with a specifi

166 our results highlight the importance of the cellulosome paradigm for cellulose and hemicellulose deg

167 These studies are the first to expand the cellulosome paradigm of protein complex assembly beyond

168 folding protein of Clostridium cellulovorans cellulosomes, possesses one family 3 cellulose binding d

169 ecause the large size and flexibility of the cellulosome preclude structural determination by traditi

170 The cellulosome produced by the S mutant strain was purified

171 In cellulosomes produced by Clostridium spp., the high-affi

172 with fibronectin domains similar to those in cellulosome-producing bacteria were also abundant, sugge

173 icity types, as generally believed for other cellulosome-producing bacteria, but reveal a more organi

174 Clostridium thermocellum is a cellulosome-producing bacterium that couples rapid repro

175 contains the genetic elements necessary for cellulosome production.

176 e of post-translational modifications of the cellulosome protein complex used by the bacterium Clostr

177 ehensive characterization, the extracellular cellulosome proteins were analyzed using multiple proteo

178 ts, 85 modified peptides corresponding to 28 cellulosome proteins were identified.

179 ve cellulolytic or structural domains of the cellulosome proteins, suggesting a level of possible reg

180 t was observed that about 15 subunits of the cellulosome reacted with anti-bCDR1 and anti-bCDR2.

181 Better understanding of the mechanism of cellulosome regulation would allow us to improve lignoce

182 mbly mechanism and functional role of fungal cellulosomes remain unknown(5,6).

183 's most elaborate multienzyme complexes, the cellulosome, results from the binding of enzyme-borne do

184 incorporate foreign enzymatic activities in cellulosomes so as to enhance lignocellulose hydrolysis

185 mplex represents the largest fragment of the cellulosome solved by x-ray crystallography to date and

186 rt of a gene cluster that also codes for the cellulosome structural components ScaA and ScaB.

187 assembly of the catalytic subunits into the cellulosome structure.

188 observed and may play a role in higher order cellulosome structure.

189 minus of the CipA scaffoldin that may aid in cellulosome substrate recognition and binding.

190 wn as CelS, S(S), and S8), the most abundant cellulosome subunit, was undertaken to gain insight into

191 bacterial cellulase systems, the multienzyme cellulosome system of the anaerobic, cellulolytic bacter

192 The cellulosome system of the human gut bacterium R. champan

193 in the human gut that produces an intricate cellulosome system of various architectures.

194 D-1 genome revealed a particularly elaborate cellulosome system.

195 s from those reported thus far for any other cellulosome system.

196 e-dockerin construct to bind more tightly to cellulosomes than a single domain and with greater cover

197 in complex from the Clostridium thermocellum cellulosome that comprises a C-terminal trimodular fragm

198 9G generated a mixture of heterogeneous mini-cellulosomes that exhibit more activity on crystalline c

199 ntributes to the assembly of R. flavefaciens cellulosome, the structures of two group 1 Docs bound to

200 Particularly fascinating are cellulosomes, the multimodular extracellular complexes p

201 In a similar experiment with EDTA-treated cellulosomes, these subunits reacted with anti-bCDR1 but

202 ures of all of the individual modules of the cellulosome, this mechanism of enzymatic synergy remains

203 dase (BglA-CohII) that binds directly to the cellulosome through an unoccupied dockerin module of its

204 f digestive enzymes is incorporated into the cellulosome through the interaction of the dockerin doma

205 t also that EngE plays an anchoring role for cellulosomes through the interaction of its dockerin dom

206 econdarily, HLDs aid the binding of the CbpA/cellulosome to the C. cellulovorans cell surface.

207 he HLDs of CbpA play a role in attaching the cellulosome to the cell surface, since they showed some

208 ay a secondary role in the attachment of the cellulosome to the cell surface.

209 rete multi-component protein networks called cellulosomes to effectively digest lignocellulosic bioma

210 lent modes of cohesin recognition to recruit cellulosomes to the cell surface, a mechanism that maxim

211 ot form large polypeptide structures, called cellulosomes, to deconstruct biomass.

212 of feruloyl esterase in the C. thermocellum cellulosome together with its other hydrolytic activitie

213 rmit improved understanding of the bacterial cellulosome using biophysical approaches, with emphasis

214 e anchoring in the Ruminococcus flavefaciens cellulosome using single-molecule force spectroscopy and

215 Docs of groups 3 and 6 are recruited to the cellulosome via a single-binding mode mechanism with an

216 The composition of the purified cellulosome was analyzed by tandem mass spectrometry wit

217 C. cellulovorans native cellulosomes were found to have higher affinity for cell

218 caG), thus enabling formation of a cell-free cellulosome, whereby ScaG interacts with a divalent scaf

219 ture produce a multiprotein complex called a cellulosome, which co-ordinates glycoside hydrolase asse

220 xtracellular multiprotein complex termed the cellulosome, which consists of >70 subunits, most of the

221 DTA at 37 degrees C, Ca is released from the cellulosome, which disintegrates into polypeptides.

222 extracellular multienzyme complex called the cellulosome, which is involved in plant cell wall degrad

223 GH3 beta-glucosidase component of the fungal cellulosome, which is thereby identified as a potential

224 cteria use large enzymatic assemblies called cellulosomes, which recruit complementary enzymes to pro

225 d into large multienzymes complexes, termed "cellulosomes," which allow for efficient hydrolysis of c

226 f a "designer" cellulosome, or a recombinant cellulosome with a specific function.

227 hown in our previous paper, treatment of the cellulosome with ethylenediaminetetraacetic acid (EDTA)

228 ) under aerobic conditions disintegrates the cellulosome with formation of truncated catalytic subuni