ライフサイエンスコーパス: centrifugation

1 thod efficiently recovered data with delayed centrifugation.

2 s with electrostatically assisted high-speed centrifugation.

3 cteria were concentrated by density gradient centrifugation.

4 of reaction product, enabling separation by centrifugation.

5 of trichloroacetic acid (TCA) and subsequent centrifugation.

6 conditions and overcome sample filtration or centrifugation.

7 further separation of both liquid phases by centrifugation.

8 further and cell fractions are separated by centrifugation.

9 cted in the bottom of the tubes under gentle centrifugation.

10 nding arc-discharge SWNTs with I followed by centrifugation.

11 via collection of the SPIONs by either F4 or centrifugation.

12 the reaction mixture by simple filtration or centrifugation.

13 eriments, and are typically achieved through centrifugation.

14 f platelet-rich plasma to about 6.5 prior to centrifugation.

15 ant female donors and plasma separated using centrifugation.

16 field-flow fraction (F4) in combination with centrifugation.

17 able saving of time and removes the need for centrifugation.

18 on their separation through sucrose gradient centrifugation.

19 ing ultracentrifugation and sucrose gradient centrifugation.

20 methacrylate), which is then recovered using centrifugation.

21 elles separated from the soluble proteins by centrifugation.

22 but were made visible after density gradient centrifugation.

23 to a 5 mL aqueous honey solution followed by centrifugation.

24 tein complex as observed by sucrose gradient centrifugation.

25 eparated from the microsomes by differential centrifugation.

26 lycogen/acetate precipitation with low-speed centrifugation.

27 was co-precipitated with bc(1) complex upon centrifugation.

28 and DRMs were isolated via sucrose gradient centrifugation.

29 a sample into 1000 nanoliter-sized wells by centrifugation.

30 red cells following sucrose density gradient centrifugation.

31 previous measurements using density gradient centrifugation.

32 l-DNA complexes at 95 degrees C, followed by centrifugation.

33 centrifugation followed by sucrose gradient centrifugation.

34 from healthy volunteers (n=4) by sequential centrifugation.

35 degrees C, undigested collagen is removed by centrifugation.

36 was used to induce emulsion breaking without centrifugation.

37 nase-pronase-perfusion, and density gradient centrifugation.

38 ered than those obtained by sucrose gradient centrifugation.

39 ng, and analytical sedimentation equilibrium centrifugation.

40 ved exosomes (EXOs) isolated by differential centrifugation.

41 ated from culture supernatants by sequential centrifugation.

42 urine samples through multistep differential centrifugation.

43 or either (b) one round or (c) two rounds of centrifugation.

44 Insoluble debris was removed by centrifugation.

45 is, immunoprecipitation, and sucrose density centrifugation.

46 particles, and their ordering is improved by centrifugation.

47 n virion proteins following sucrose gradient centrifugation.

48 sions by MS while circumventing dialysis and centrifugation.

49 Mouse BMCs were isolated by density-gradient centrifugation.

50 ies would normally be removed by dialysis or centrifugation.

51 plasma from whole blood without the need for centrifugation.

52 ted into cellular and acellular fractions by centrifugation.

53 anical pressing, heating to 50 degrees C and centrifugation.

54 ample preparation such as filtration step or centrifugation.

55 which protein was separated from fat through centrifugation.

56 of the liver and subsequent density-gradient centrifugation.

57 cle fractions from differential and gradient centrifugations.

58 y purified from mature seeds by differential centrifugations.

59 After centrifugation, 1 muL of the sedimented phase was analys

60 B were heavy and readily settled down under centrifugation (13,000rpm, 60min).

61 with sodium sulfide following high-velocity centrifugation (21500g, 45 min) at 4 degrees C.

62 d small decreases in specificity (for bleach centrifugation, -3% [95% CI = -4% to -1%, P = 0.004]; fo

63 o laser engineered micromoulds by high-speed centrifugation (30min) to concentrate NMP into MN shafts

64 o small increases in sensitivity (for bleach centrifugation, 6% [95% confidence interval [CI] = 3 to

65 es by Blue Native PAGE, Suc density gradient centrifugation, 77K fluorescence, circular dichroism, an

66 The final biphasic system was separated by centrifugation (8 min, 10,000 rpm).

67 nducted without manual operations, including centrifugation, a widely used means of isolating a solid

68 heres can be easily removed by filtration or centrifugation after extraction reaction.

69 is useful to remove these nanomaterials via centrifugation after reacting with spin trapping agents.

70 ies (11 papers) included, 9 evaluated bleach centrifugation and 5 evaluated bleach sedimentation.

71 iple rounds of differential density gradient centrifugation and analyzed by immunoelectron microscopy

72 Using sucrose density gradient centrifugation and antibody pulldowns, we show that all

73 vesicles have been isolated by differential centrifugation and are characterized further by mass spe

74 evidence was rated very low for both bleach centrifugation and bleach sedimentation.

75 Two different curd drainage methods (centrifugation and Buchner funnel filtration) were used

76 developed and validated based on systematic centrifugation and comparison of colony forming unit (CF

77 tage, followed by a subsequent sonification, centrifugation and concentration step.

78 size and zeta-potential were prepared using centrifugation and consequently employed for BOD-ErGO bi

79 d lipids with acetonitrile:water (3:2, v/v), centrifugation and continuous solid-phase extraction for

80 equires minimal sample preparation (a single centrifugation and dilution), and can be completed, from

81 Instead, sucrose density gradient centrifugation and electron microscopy showed that BFA a

82 the AAVS1 site, we show by using analytical centrifugation and electron microscopy that the full-len

83 ns (F151A) was confirmed to be monomeric via centrifugation and exhibited a weakened ability to bind

84 es were pretreated by protein precipitation, centrifugation and filtration, followed by HPLC separati

85 Compared with traditional methods based on centrifugation and filtration, the established method is

86 Using gradient centrifugation and fluorescence-activated cell sorting,

87 re isolated from Giardia by density gradient centrifugation and found to be sensitive to nystatin and

88 racts were also subjected to sucrose density centrifugation and fractions analyzed by BNGE or enzymat

89 Removal of the precipitate via centrifugation and identification of the protein content

90 rnatants and exosomes was purified by serial centrifugation and immunoprecipitation with magnetic bea

91 Consistent with these data, sucrose gradient centrifugation and indirect immunofluorescence microscop

92 ose tissue were purified by sucrose gradient centrifugation and labeled with green fluorescent dye an

93 e UvsY heptamer was confirmed in solution by centrifugation and light scattering, and thermodynamic a

94 pernates and exosomes was purified by serial centrifugation and magnetic beads, and DEK's posttransla

95 as examined in ES cell lines by differential centrifugation and membrane solubilization by digitonin.

96 s of different accessions; 2) the effects of centrifugation and microwave treatment on extracting oil

97 The combined use of centrifugation and microwave treatments gave high oil ex

98 mically large structures in density gradient centrifugation and native gel electrophoresis.

99 astringency removal, flash vacuum expansion, centrifugation and pasteurization) in order to evaluate

100 and centrifugation, and astringency removal, centrifugation and pasteurization, respectively) contain

101 onocytes isolated by Ficoll-Hypaque gradient centrifugation and plastic adherence were incubated with

102 ng two methods for EV recovery, differential centrifugation and polyethylene glycol precipitation fol

103 t pre-treatment of the samples, e.g. without centrifugation and precipitation of protein with acids.

104 samples extracted by two different methods (centrifugation and pressed processing) were characterize

105 In conclusion, this method does not require centrifugation and produces a clean spectrum for VMS ana

106 ecialized vials by a phlebotomist along with centrifugation and refrigerated storage.

107 t rely on precipitation of a urine pellet by centrifugation and requires a smaller volume of urine.

108 CAS caused a rapid ATPS without resorting to centrifugation and salt, mainly because of strong electr

109 s in urine were analyzed by fractionation by centrifugation and separation by sodium dodecyl sulfate

110 ere used; six honey samples were obtained by centrifugation and six by honeycomb press.

111 We used differential centrifugation and size-exclusion chromatography as orth

112 rphic lamellar structures by buoyant density centrifugation and subsequent SDS-PAGE and proteomics id

113 In this study, a combination of density centrifugation and surface charge separation techniques

114 Crude peptides were separated by high-speed centrifugation and tested for antioxidant and antimutage

115 cols to fulfill these tasks require multiple centrifugation and vacuum-centrifugation steps, making l

116 ellar genomes and metabolites are removed by centrifugation and washing, thus maximizing the utility

117 t in a couple of hours without requiring any centrifugation and/or vacuum centrifugation steps.

118 Combined physical (centrifugation) and chemical (molybdate reactiveness) fr

119 droplet size following exposure to physical (centrifugation) and thermal stressors (freeze, thaw cycl

120 reated donor cells (Donor cells) using ultra-centrifugation, and analyzed for acetylcholinesterase ac

121 rgoing the combinations of clarification and centrifugation, and astringency removal, centrifugation

122 c acid to release the protein-bound vitamin, centrifugation, and mixing of the supernatant with phosp

123 structured-illumination microscopy, gradient centrifugation, and proteasome inhibition approaches rev

124 on as well as the aqueous phase separated by centrifugation, and quantification by absorption spectro

125 ltered sedimentation during sucrose gradient centrifugation, and retarded mobility during native agar

126 rger colloids prior to analysis (filtration, centrifugation, and sedimentation).

127 Live imaging of Hyal1, sucrose gradient centrifugation, and specific colocalization of Rab GTPas

128 The sample pretreatment only involved centrifugation, and the time required for lipid quantifi

129 nge style patch, extracted from the gauze by centrifugation, and then derivatized using CIL.

130 reverse ionotropic gelation and purified by centrifugation, and then total RNA was extracted using h

131 ce were collected from the initial sample by centrifugation, and they were then detached from the NP

132 d MUC2-N by gel filtration, density gradient centrifugation, and transmission electron microscopy.

133 ight with zirconium oxychloride, followed by centrifugation, and washed twice with water followed by

134 Electron microscopy, gradient centrifugation, and Western blot analysis indicated that

135 We have developed a novel blood lysis-centrifugation approach for highly sensitive Mycobacteri

136 The use of centrifugation as assistive technology appears to be nec

137 on net actin polymerization as determined by centrifugation assays.

138 Here we show a centrifugation-assisted assembly of colloidal silica sph

139 d with ultra-high quality water and applying centrifugation-assisted ultrafiltration (CUF).

140 by homogenization at 15,000 rpm followed by centrifugation at 3000 rpm.

141 a) has been developed, comprising 2 steps: a centrifugation at low temperature to eliminate more than

142 We subsequently incorporated a centrifugation-based fractionation step to deplete the h

143 The technology replaces centrifugation-based separation with a faster and highly

144 A standard centrifugation-based water retention capacity, swelling

145 Through centrifugation, bilayer SnS nanosheets can be produced s

146 itive blood culture for Candida in the lysis-centrifugation blood culture system (Isolator, Wampole L

147 alphacat in the supernatant after high-speed centrifugation, but the protein still elutes in the void

148 tric concentration is typically achieved via centrifugation, but this technique is not well suited fo

149 rom the bulk of serum proteins by high-speed centrifugation causing substantial sample enrichment pri

150 Iterative centrifugation cycles at low centripetal force not only

151 The most robust assay involved an automated centrifugation-decantation step to remove latex aggregat

152 maller nanoparticles (diameter <150 nm), the centrifugation-decantation step was not required as the

153 nce of soluble solid separated by high speed centrifugation denoted the presence of lycopene in this

154 om blood of melanoma patients using a simple centrifugation device (OncoQuick), and 4 melanocyte targ

155 n separated from the small molecules using a centrifugation device.

156 Physical partitioning by density gradient centrifugation did not separate phyllosilicate and Fe(II

157 nents were determined using a combination of centrifugation, drying, gas pycnometry and image analysi

158 ty to mediate fast diffusion of VX and a MAS centrifugation effect.

159 Rate-dependence of centrifugation efficiency for each component suggests di

160 ecovering the oil bodies from the extract by centrifugation, either in the presence of sucrose or by

161 to salivary gland debris by density gradient centrifugation eliminated salivary gland-associated inhi

162 Innovations such as centrifugation-enhanced shell vial and multiwell plate c

163 sed bovine ROS membranes by sucrose gradient centrifugation exhibited improved stability and the expe

164 Following isopynic centrifugation, exosomal proteins flotillin-1 and TSG101

165 By combining sucrose gradient centrifugation experiments, limited proteolysis, one-dim

166 We relate, by simple analytical centrifugation experiments, the density of colloidal flu

167 Stopping the reaction with acetic acid and a centrifugation/filtration step to decrease turbidity are

168 on of the milk sample using acetonitrile and centrifugation followed by a solid-phase extraction (SPE

169 removed completely or to a trace level after centrifugation followed by filtration which minimises it

170 Using repeated differential centrifugation followed by Percoll gradient fractionatio

171 e foods, an extraction of the supernatant by centrifugation followed by quantitative determination us

172 sheared chromatin to sedimentation velocity centrifugation followed by size-fractionation of the DNA

173 purified from Syn5-infected cells using CsCl centrifugation followed by sucrose gradient centrifugati

174 ia rapidly and inexpensively using low-speed centrifugation, followed by a single small-volume ultrac

175 acidified methanol, ultrasound treatment and centrifugation, followed by an immunoaffinity column (IA

176 ated by nitrogen cavitation and differential centrifugation, followed by free flow electrophoresis.

177 (HCl or H2SO4), followed by separation using centrifugation for dissolved coagulant recovery.

178 We demonstrate the use of centrifugation for efficient separation of colloidal gol

179 lays markedly reduced in vitro dependence on centrifugation for infection and low incidence and sever

180 It relies on a simple but controllable centrifugation force for the actuation of samples and re

181 Furthermore, our data from differential centrifugation, Forster resonance energy transfer, nativ

182 parison of proteins in CsCl density gradient centrifugation fractions from supernatants of wild-type

183 ls (PBMCs) were isolated by density gradient centrifugation from the blood of patients with primary c

184 teria and EV from samples using differential centrifugation, genomic DNA was extracted and 16S-rDNA a

185 Centrifugation had no effect on DNA availability, wherea

186 However, common concentration by centrifugation has important limitations when processing

187 simplicity, and versatility, single-molecule centrifugation has the potential to expand single-molecu

188 heating (i.e., manual) and by chromatography/centrifugation (i.e., column) methods.

189 sing in vivo crosslinking, velocity gradient centrifugation, immuno-isolation, and tandem mass spectr

190 Using sucrose gradient centrifugation, immunoadsorption and immunofluorescence

191 Enzymatic assays, sucrose density gradient centrifugation, immunoprecipitation, dot and Western blo

192 Cell homogenization and sucrose gradient centrifugation in combination with transferrin-HRP/DAB a

193 parating dissociated liver cells by gradient centrifugation into two portions, hepatocytes (parenchym

194 ught into close proximity to target cells by centrifugation, intoxication was increased 4-fold.

195 Cell concentration via centrifugation is a ubiquitous step in many cell culture

196 atories to separate plasma from whole blood, centrifugation is generally inappropriate for on-site te

197 el underpinning the self-ordering process by centrifugation is proposed to describe the interplay bet

198 Percoll density centrifugation is simple, can be done on the day of tran

199 Sputum centrifugation may increase the yield of Xpert MTB/RIF.

200 of each sample were processed by a new lysis-centrifugation method and tested with the Xpert MTB/RIF

201 is presented and compared to an established centrifugation method for analytics.

202 n capacity as determined by a novel drainage centrifugation method, and Farinograph absorption were n

203 Compared to the centrifugation method, use of the novel magnetic sulfate

204 electrode measurements and/or a conventional centrifugation method.

205 During centrifugation, nanoparticles undergo Brownian motion un

206 Following centrifugation, O-glycans are recovered in the filtrate.

207 actors using fractions derived from gradient centrifugation of a scrapie-susceptible cell line.

208 es were observed by sucrose density gradient centrifugation of bacterial extracts during mid-logarith

209 bility was higher in subjects with immediate centrifugation of blood samples.

210 y polyhedrocyte formation from the forces of centrifugation of blood without clotting.

211 Centrifugation of buttermilk (5% total solids, TS)--curc

212 Density gradient centrifugation of isolated exosomes revealed the presenc

213 Density gradient centrifugation of L1 ribonucleoprotein particles, subcel

214 he volume of the pellet obtained by benchtop centrifugation of nanomaterial suspensions in a packed c

215 by coating MPs with human ES cell lysate and centrifugation of specific ratios of ES cells and MPs to

216 ybeans; (ii) homogenisation, suspension, and centrifugation of the filter cake.

217 This activity could be attenuated by either centrifugation of the inhibitor stock solution or by add

218 able exception of sample volume reduction or centrifugation, one of the most widely utilized processe

219 However, centrifugation only removed a small fraction of total pa

220 k up the emulsion (that otherwise would need centrifugation or a demulsification solvent), further im

221 le cells require the use of density gradient centrifugation or antibody-based cell sorting with molec

222 cell survival without enzyme neutralization, centrifugation or drug treatment.

223 CNT-Thy, simply recycled by centrifugation or filtration, can be redispersed in anot

224 icity of the protocol used, with no need for centrifugation or filtration, organic solvents or high t

225 Ribosomes were obtained by differential centrifugation or immunopurification and were digested w

226 This aggregation was not affected by centrifugation or pretreatment with organic solvents.

227 ds for quantifying persulfate require either centrifugation or prolonged settling times.

228 2 (P1), 1:1 dilution with 2% HNO3 and latter centrifugation (P2) or filtration (P3) and direct analys

229 Dilution with 2% HNO3 and centrifugation (P2) provided the best results, i.e. LODs

230 or filtration (P3) and direct analysis after centrifugation (P4) for the determination of total Al, B

231 oth within and outside of the 16.5 and 120 K centrifugation pellets which contain most of the known c

232 the PPT for 6 h at room temperature prior to centrifugation (PPT6H) rather than 2 h as specified in t

233 erwent homogenization, lipid hydrolysis, and centrifugation prior to extraction.

234 Density gradient centrifugation procedures reduce the level of impurities

235 NARE is the elimination of dilutive wash and centrifugation processes that are fundamental to convent

236 However, in centrifugation, proteins with good affinity to the SPION

237 Nuclei displaced by centrifugation rapidly recentered by mechanisms that dep

238 ddition of acetonitrile to the milk samples, centrifugation, removal of matrix by SPE, concentration

239 Centrifugation removes large cardiomyocytes and tissue d

240 Sucrose gradient centrifugation revealed chaperonin-sized complexes of bo

241 Sucrose gradient density centrifugation revealed that EaDAcT colocalizes to the s

242 irculating mRNAs by sucrose density gradient centrifugation revealed that the liver-specific mRNAs we

243 After 8.5min of centrifugation, sedimented phase (surfactant-rich phase)

244 Sucrose density gradient centrifugation showed that exposure of roots to 0.5 mM H

245 led precursor procapsids by sucrose gradient centrifugation shows that there appear to be three class

246 Differential centrifugation, size-exclusion chromatography, and prote

247 mple pH, ionic strength, extraction time and centrifugation speed were systematically investigated.

248 hysically stable when subjected to different centrifugation speeds.

249 ables direct film preparation after a simple centrifugation step.

250 d nanospheres are purified using traditional centrifugation steps and/or anion exchange chromatograph

251 organic solvents and time-consuming multiple centrifugation steps that are required in traditional RN

252 f EC cultures were subjected to differential centrifugation steps to collect larger and smaller EVs w

253 s require multiple centrifugation and vacuum-centrifugation steps, making liquid handling robot media

254 t requiring any centrifugation and/or vacuum centrifugation steps.

255 out requiring the use of organic solvents or centrifugation steps.

256 Density gradient centrifugation studies reveal that these replication-tra

257 At the macroscale, centrifugation suffers from a number of limitations, par

258 Sucrose-gradient centrifugation suggested that caveolin-1 closely associa

259 glutaraldehyde cross-linking and rate zonal centrifugation suggested that EspD in LUV membranes form

260 procedure, and that of the Isolator 10 lysis-centrifugation system in the detection of Mycobacterium

261 equipped with "two-phase" and "three-phase" centrifugation systems, respectively.

262 Commonly used differential centrifugation techniques produce impure fractions and m

263 Using electron microscopy and analytical centrifugation techniques, we observed spontaneous foldi

264 Rolling circle amplification and gradient centrifugation testing were positive for encapsidated HP

265 nd that more pronounced order is obtained by centrifugation than sedimentation.

266 ction factor governing the effective time of centrifugation that is pertinent in the analysis of boun

267 This was interpreted to mean that, during centrifugation, the spherical ghosts were oriented by a

268 y, by dehydration of silica particles during centrifugation, the spontaneous formation of capillary w

269 aqueous micelle phase has been separated by centrifugation, then it was treated with a mixture of H2

270 hSOD1 aggregate seeds that were prepared by centrifugation through a density cushion were inoculated

271 ration of the chelating ligand, salt effect, centrifugation time and sample volume were investigated

272 t, sample pH, salting-out effect, vortex and centrifugation time were studied.

273 l that uses simple and rapid Percoll density centrifugation to enrich RV-susceptible activated CD8(+)

274 We used density gradient centrifugation to isolate LDL in plasma pooled from 5 no

275 Here, we used gentle centrifugation to obtain a mucus supernatant showing no

276 ilizes Balch homogenization and differential centrifugation to obtain two distinct vesicular fraction

277 the exfoliation of layered MFI, followed by centrifugation to remove non-exfoliated particles.

278 However, centrifugation to remove SWCNTs and adsorbed EE2 signifi

279 % NaOH at 30 degrees C for 30min followed by centrifugation to separate the starch rich and the prote

280 periments demonstrate the viability of using centrifugation to shape-separate a mixture of colloidal

281 ribosome-bound species) with sucrose density centrifugation (to recover intact 70S monosomes).

282 Preparation of the complex involved centrifugation (to remove insoluble iron), ultrafiltrati

283 brain extracts were subjected to high speed centrifugation, to remove insoluble alpha-syn aggregates

284 tal Exosome Isolation Kit or by differential centrifugation/ultracentrifugation.

285 xtract was size fractionated by differential centrifugation/ultrafiltration for analysis of P, Al, Fe

286 ith all cross-linked proteins is isolated by centrifugation under denaturing conditions.

287 ssociated hESCs in the absence of ROCK-i and centrifugation was achieved within an optimal range of i

288 Rho-associated kinase inhibitor (ROCK-i), or centrifugation was needed and the side effects of ROCK-i

289 Centrifugation was required in 59% cases with scanty spu

290 ubated ex vivo +/- insulin, and differential centrifugation was used to isolate cytosolic and membran

291 tion of the CCM, as measured by silicone oil centrifugation, was hindered in the presence of rifampin

292 by dynamic light scattering and differential centrifugation, was less straightforward.

293 assessed using the Enslin-Neff and drainage centrifugation water retention capacity methods, thermog

294 ffinity chromatography and glycerol gradient centrifugation, we show that TFIIB associates with poly(

295 olated by immunoaffinity and sucrose cushion centrifugation were characterized by morphologic, bioche

296 ue membrane pellet obtained after high-speed centrifugation, whereas proteins that are weakly associa

297 e purification approach utilizing rate-zonal centrifugation, which provides comparable separation res

298 ferent individuals (n=3) submitted or not to centrifugation (whole vs clarified saliva).

299 y TaqMan, there was poor concordance without centrifugation with the TaqMan platform with only 40% 'u

300 sedimented organic phase was obtained after centrifugation, withdrawn into the microsyringe and dire