ライフサイエンスコーパス: centrin

1 nown to be important in the interaction with centrin.

2 lead us to propose a novel binding motif for centrin.

3 erminal domain serves as a calcium sensor in centrin.

4 rahymena EF hand proteins are different from centrin.

5 tains all of the defining characteristics of centrin.

6 and all of the structural features common to centrin.

7 ired direct binding to the centriole protein centrin.

8 body component is the Ca(2+)-binding protein centrin.

9 cation without segregation in the absence of centrin.

10 e have previously shown that the Tetrahymena centrin 1 (Cen1), a human centrin 2 homologue, is requir

11 Here, the mouse centrin 1 gene (Cetn1) is analyzed with respect to its g

12 In this paper, we show that the Tetrahymena centrin 2 (Cen2), a human centrin 3 homologue, has funct

13 rylation sites within the centriolar protein Centrin 2 (Cetn2).

14 two-hybrid screening using both Homo sapiens centrin 2 (Hscen2) and Chlamydomonas reinhardtii centrin

15 We previously identified centrin 2 and centrin 3 (Cetn2 and Cetn3) as substrates

16 YS/MEL-28 resulted in a specific decrease of centrin 2 at the nuclear rim of HeLa cells.

17 C-terminal calcium-binding domains of human centrin 2 caused a dominant-negative effect on both mRNA

18 at the Tetrahymena centrin 1 (Cen1), a human centrin 2 homologue, is required for proper basal body f

19 hey function in a manner distinct from human centrin 2 homologues.

20 We conclude that in vertebrates, centrin 2 interacts with major subunits of the nuclear p

21 n vitro reconstituted nuclei indeed revealed centrin 2 localized at the nuclear pores.

22 orescence microscopy demonstrates that mouse centrin 2 protein localizes to the region immediately su

23 Unexpectedly, we found centrin 2 to associate biochemically with nucleoporins,

24 digitonin in immunofluorescence also allowed centrin 2 to be clearly visualized at the nuclear pores

25 hat Leishmania centrin is a homolog of human centrin 2.

26 ps based on sequence similarity to the human centrins 2 and 3.

27 a pigmentosum group C peptides both bound to centrin-2 also interact to form an alpha-helical coiled-

28 The interface between centrin-2 and each peptide is predominantly nonpolar, an

29 To determine the structure of human centrin-2 and to develop an understanding of molecular i

30 e shows that the carboxyl-terminal domain of centrin-2 binds this peptide and two calcium atoms, wher

31 stal structure of calcium-loaded full-length centrin-2 complexed with a xeroderma pigmentosum group C

32 riolar protein centrin-2: reducing levels of centrin-2 in HeLa cells has been found to block centriol

33 Human centrin-2 plays a key role in centrosome function and st

34 Following inhibition of centrin-2 synthesis, the preexisting pair of centrioles

35 XPC/hHR23 was also reported to bind centrin-2, a member of the superfamily of calcium-bindin

36 rference, we demonstrate that "knockdown" of centrin-2, a protein of centrioles, results in failure o

37 and induced modifications of pericentrin and centrin-2, two essential proteins required for centrosom

38 light on the role of the centriolar protein centrin-2: reducing levels of centrin-2 in HeLa cells ha

39 We previously identified centrin 2 and centrin 3 (Cetn2 and Cetn3) as substrates of the protein

40 at the Tetrahymena centrin 2 (Cen2), a human centrin 3 homologue, has functions similar to Cen1 in ba

41 A further examination of human centrin 3 homologues shows that they function in a manne

42 bodies between the two nuclei together with centrin, a basal body/centrosome-specific protein.

43 Cdc31p is the yeast homologue of centrin, a highly conserved calcium-binding protein of t

44 protein that has a role in protein export is centrin, a member of the EF-hand superfamily of Ca(2+)-b

45 Antibodies to centrin abrogated this increase.

46 assembly begins with the formation of small centrin aggregates that appear during the S phase.

47 Centrin aggregations, resembling blepharoplasts, occur i

48 Recent findings suggest that centrin also functions elsewhere in the cell.

49 Centrin, an EF hand Ca(2+) binding protein, has been clo

50 00 kDa to 3 MDa) complexes that contain both centrin and CaM.

51 stages, first losing gamma-tubulin and then centrin and glutamylated tubulin.

52 Sfi1 C-terminal centrin-binding repeats, and centrin and Kar1 provide cross-links, while Sfi1-CT stab

53 ment of proteins to the centrosome, of which centrin and ninein are required for interphase microtubu

54 continuously exchanges at basal bodies, and centrin and Sas6a exhibit both of these patterns.

55 The bimodal dynamics found for centrin and Sas6a reveal unique protein assembly mechani

56 erstanding of molecular interactions between centrin and xeroderma pigmentosum group C protein, we ch

57 These observations show that centrin and XPC cooperate in a reciprocal mechanism to c

58 It has > 80% homology to other centrins and high homology to Tetrahymena EF hand protei

59 rved centrosomal constituents gamma-tubulin, centrin, and MPM-2 (which detects phosphorylated epitope

60 t that GRK5 co-localizes with gamma-tubulin, centrin, and pericentrin in centrosomes.

61 our proteins analyzed (alpha-tubulin, Spag6, centrin, and Sas6a).

62 g the double staining with alpha-tubulin and centrin antibodies strongly suggested that centrin is as

63 crotubule distributions in spermatids, since centrin appears to control formation of the cytoskeleton

64 retch of the amino-terminal domain unique to centrins appears disordered.

65 Centrins are a family of small, calcium-binding proteins

66 Centrins are a ubiquitous family of small Ca(2+)-binding

67 Centrins are ancient calmodulin-related Ca(2+)-binding p

68 Centrins are calcium binding proteins involved in cell d

69 Centrins are calcium binding proteins that belong to the

70 Centrins are calmodulin-like proteins present in centros

71 Centrins are calmodulin-like proteins present in microtu

72 Centrins are cytoskeleton proteins with key roles in cel

73 Centrins are highly conserved components of the centroso

74 -like kinase (TbPLK), which is essential for centrin arm and FAZ duplication.

75 s that include the hook complex (HC) and the centrin arm.

76 In Trypanosoma brucei, three of the five centrins associate with the flagellar basal body, but no

77 suggest that translational blocks induced by centrin-based RNA are gene specific and concentration de

78 ein (GANP) scaffold to which ENY2, PCID2 and centrins bind and depletion of any of these components i

79 trin in the repeat region, showing that this centrin-binding motif is conserved.

80 is restricted to Sfi1-CT and Sfi1 C-terminal centrin-binding repeats, and centrin and Kar1 provide cr

81 This centrin-binding site is highly conserved within the firs

82 We identified a centrin-binding site within H. sapiens Prp40 homolog A (

83 In vitro, the C-terminal domain of centrin binds to the yeast centrosomal protein Kar1p in

84 -centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible w

85 relative stability of the helical regions of centrins by circular dichroism.

86 ine the stepwise molecular behavior of human centrins by two-dimensional infrared (2D IR) correlation

87 hermally induced molecular behavior of human centrins can be used to predict biological target intera

88 ts are the membrane-anchored Kar1, the yeast centrin Cdc31, and the Cdc31-binding protein Sfi1.

89 prising binding partner, the calcium-binding centrin Cdc31, in trans-Golgi network (TGN) to endosome

90 ngs reveal for the first time a new role for centrin/Cdc31 in protein degradation.

91 s that found for the Saccharomyes cerevisiae centrin Cdc31p at the yeast nuclear pore, a role until n

92 The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repea

93 The Saccharomyces cerevisiae centrin, Cdc31p, was functionally tagged with a single Z

94 Centrin/Cdc31p is a Ca2+-binding protein related to calm

95 e-stranded RNA probes made from the Marsilea centrin cDNA, MvCen1, to block centrin translation.

96 trins wrapped around each repeat and similar centrin-centrin contacts between each repeat.

97 ectron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bou

98 The structure of the yeast Sfi1-centrin complex, and its asymmetric position within the

99 The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p

100 he flagellum, which may be shared by all the centrin-containing flagellated and ciliated organisms.

101 rin 2 (Hscen2) and Chlamydomonas reinhardtii centrin (Crcen).

102 In situ hybridizations reveal that centrin, cyclin B, and beta-tubulin mRNAs are present in

103 live attenuated Leishmania vaccines such as centrin deleted Leishmania donovani parasites (LdCen (-/

104 ted Rab6 on Golgi membranes colocalized with centrin during mitosis, and parasite clones expressing R

105 ority of known centriole proteins, including centrin, epsilon tubulin, and the cartwheel protein BLD1

106 iation, the spermatid nuclei remained round, centrin failed to localize into basal bodies, thus block

107 on of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge

108 terminal domain of Chlamydomonas reinhardtii centrin for calcium and for a peptide fragment of Kar1p

109 Two KIC-related Ca2+ binding proteins and a centrin from Arabidopsis, which contain one and four EF-

110 Our data suggest that basal bodies require a centrin from both groups in order to function correctly.

111 the order Kinetoplastida a gene encoding for centrin from L. donovani.

112 his is the first demonstration of a specific centrin function associated with axonemal dynein.

113 Analyses of centrin function suggest a role in basal body assembly a

114 cts resulting from ablation of either CaM or centrin function.

115 ania, we created Leishmania deficient in the centrin gene (LdCEN).

116 A mutation in the centrin gene also reduced the rate of templated assembly

117 is is the first report where disruption of a centrin gene displays stage-specific/cell type-specific

118 (-/-) parasites, in addition to loss of the centrin gene, have additional deletions ranging from 350

119 is study addresses whether a live attenuated centrin gene-deleted L. donovani (LdCen1(-/-)) parasite

120 reported protective role of live attenuated centrin gene-deleted Leishmania donovani (LdCen(-/-) ) p

121 We had shown that disruption of one of the centrin genes (centrin1) in Leishmania amastigotes resul

122 phylogenetic relationship to the other mouse centrin genes and their human orthologs.

123 trahymena thermophila contains at least four centrin genes as determined by sequence homology, and th

124 er understanding of the functions of the two centrin groups and to determine their potential redundan

125 erminal domain of Chlamydomonas rheinhardtii centrin has been determined in the presence of calcium b

126 ociate with the flagellar basal body, but no centrin has been found to regulate flagellar motility.

127 binding partner for the Ca2+-binding protein centrin has provided new insight into the dynamic behavi

128 Previously centrins have been implicated only in microtubule-based

129 duplication is to recruit Cdc31p, the yeast centrin homologue, to the half-bridge.

130 The function of centrin in Ca(2+) control of IAD activity was explored u

131 These results demonstrate a requirement for centrin in centriole duplication and demonstrate that ce

132 ation, function, and evolutionary history of centrin in higher eukaryotes.

133 Expression of N-terminal-deleted centrin in the parasite significantly reduces its growth

134 To explore the role of centrin in the protozoan parasite Leishmania, we created

135 n protein containing Sfi1 repeats also binds centrin in the repeat region, showing that this centrin-

136 for the first time a new and unique role for centrin in the segregation of organelles without affecti

137 f phosphorylation of the centrosomal protein centrin in this process.

138 our knowledge, that HsPrp40Ap interacts with centrin in vitro, supporting a coupled functional role f

139 uggesting that this kinase can phosphorylate centrin in vivo.

140 e opportunity to further explore the role of centrins in cell division in malaria parasites and sugge

141 able to decipher the evolutionary history of centrins in eukaryotes with particular emphasis on the s

142 Centrins in vertebrates have traditionally been associat

143 Cetn3) encodes the calcium-binding protein, centrin, in the mouse.

144 Ca2+-binding proteins, calmodulin (CaM) and centrin, in vivo.

145 Analysis of the three other centrins indicates that two of them function at microtub

146 31 cells do not express XPC and fail to move centrin into the nucleus following DNA damage.

147 Centrin is a calcium-binding centrosome/basal body-assoc

148 Centrin is a calcium-binding cytoskeletal protein essent

149 Centrin is a calcium-binding cytoskeletal protein involv

150 Centrin is a centrosome component in species from yeast

151 Centrin is a conserved component of centrioles in animal

152 d immunoreactivity confirmed that Leishmania centrin is a homolog of human centrin 2.

153 It suggests that centrin is a key regulatory protein for Tetrahymena axon

154 Centrin is a low molecular mass (20 kDa) protein that be

155 function in the duplication of basal bodies, centrin is also important for the integrity of these org

156 Centrin is an EF-hand calcium-binding protein closely re

157 Centrin is an essential component of microtubule-organiz

158 In asking whether centrin is an essential component of the blepharoplast,

159 d centrin antibodies strongly suggested that centrin is associated with the parasite centrosome.

160 organizing center, our results suggest that centrin is constitutively bound to Kar1p through its C-t

161 mmunoelectron microscopy studies showed that centrin is expressed in close proximity with the nucleus

162 Centrin is found in the proximal sperm centrosomal regio

163 Centrin is localized to basal bodies, cortical fibers in

164 We show that centrin is phosphorylated at serine residue 170 during t

165 Caltractin (centrin) is a member of the calmodulin (CaM) superfamily

166 Caltractin (centrin) is a member of the calmodulin subfamily of EF-h

167 ifferent species suggest that they contain a centrin isoform from each group.

168 episomal expression of the four PfCENs in a centrin knock-out Leishmania donovani parasite line that

169 Centrin loads and exchanges at the basal body distal end

170 NuMA, gamma-tubulin, and centrin localize to each pole, and nocodazole treatment

171 These studies indicate that centrin may have a functional role in Leishmania growth.

172 Sfi1 binds to multiple centrin molecules along a series of internal repeats, an

173 The levels of centrin mRNA and protein were high during the exponentia

174 ntrated in spermatogenous initials, and that centrin mRNA is translated only in spermatogenous initia

175 nick end labeling positivity was observed in centrin mutant axenic amastigotes compared with wild typ

176 An NMR titration of the centrin N-terminal domain with a fragment of the known c

177 The centrin null mutant defective in amastigote growth could

178 Remarkably, centrin null mutants (LdCEN(-/-)) showed selective growt

179 The typical mitotic spindle with centrin-, odf2-, kinesin-12-, and CP110-positive centros

180 otubule organization was also observed after centrin or ninein depletion.

181 smodium possesses orthologs of four distinct centrin paralogs traceable to the ancestral alveolate, i

182 All approaches led to reduced targeting of centrin, pericentrin, and ninein to the centrosome.

183 characterize the four Plasmodium falciparum centrins (PfCENs) and, by growth complementation studies

184 ultured cells undergo a dramatic increase in centrin phosphorylation following the experimental eleva

185 on of centrosomes at prophase and implicates centrin phosphorylation in centriole separation that nor

186 Taken together, these results suggest that centrin phosphorylation signals the separation of centro

187 These results show that centrin plays an essential role in the formation of a mo

188 cellular targets and the distinct nature of centrin relative to other EF-hand proteins.

189 mparison of the N- and C-terminal domains of centrin reveals a structural and biochemical basis for t

190 and centrosomal proteins, gamma-tubulin and centrin, rose significantly in female ACI rat mammary gl

191 Kar1p through its C-terminal domain and that centrin's calcium sensor activities are mediated by the

192 eeper insights into the structural basis for centrin's function, we have characterized the affinities

193 sly proposed model, the C-terminal domain of centrin serves as a constitutive anchor to target protei

194 Centrins, small calcium binding EF-hand proteins, functi

195 A centrin-specific antibody demonstrates that the ends of

196 Prp40 was found to be a centrin target by yeast-two-hybrid screening using both

197 terminal domain with a fragment of the known centrin target Sfi1 reveals binding of the peptide to a

198 d suggest that CP110 cooperates with CaM and centrin to regulate progression through cytokinesis.

199 Centrin translation accompanies blepharoplast appearance

200 The disruption of centrin translation affects microtubule distributions in

201 The alpha-tubulin protein distribution, centrin translation, and Mv-PRP19 mRNA distribution are

202 r beta-tubulin- nor HIV-derived RNAs affects centrin translation.

203 the Marsilea centrin cDNA, MvCen1, to block centrin translation.

204 Electrophoretic mobility shift of centrin treated with EGTA and abrogation of the shift in

205 teractions between cations and Chlamydomonas centrin using Fourier transform infrared (FT-IR) and cir

206 stablishes the relative stability of related centrins via complementary biophysical techniques.

207 The alpha-helical content in centrin was determined to be 60%-53% in the presence and

208 Calcium binding site IV in C. reinhardtii centrin was found to bind Ca2+ approximately 100-fold mo

209 To elucidate the functions of yeast centrin, we carried out a two-hybrid screen for Cdc31p-i

210 We report here that yeast centrin, which is encoded by CDC31, is similarly present

211 al repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-