ライフサイエンスコーパス: centriolar

1 hat gamma-tubulin is mainly localized in the centriolar adjunct from which an aster of microtubules e

2 Centriolar ALMS1 expression was retained into maturity.

3 Here we demonstrate that the centriolar and basal body protein HYLS-1, the C. elegans

4 ence centrosomes possess increased levels of centriolar and pericentriolar components including gamma

5 Thus, both centriolar and pericentriolar material proteins contribu

6 -tubulin is a component of the basal foot, a centriolar appendage that connects centrioles to the api

7 indicates that centriolin localizes Evi5 to centriolar appendages to turn off centrosomal Rab11 acti

8 tures were present, supporting the idea that centriolar assembly starts with the formation of a tube-

9 Assembly of SAS-6 dimers to form the centriolar cartwheel requires the ZYG-1/Plk4 kinase.

10 EP19 is recruited to the ciliary base by the centriolar CEP350/FOP complex and then specifically capt

11 Centriolar coiled coil protein 110 (CP110) caps the dist

12 We show that cea encodes the centriolar coiled-coil protein Sas-6, and that zebrafish

13 trated that an Ana2-dynein light chain (LC8) centriolar complex is critical for proper spindle positi

14 nas, mutation of the gene encoding the novel centriolar component Bld10p results in seemingly acentri

15 investigate the molecular role of the mother centriolar component Cep164 in ciliogenesis.

16 w study shows that PLK4 phosphorylates a key centriolar component, Ana2/STIL, to initiate centriole a

17 proximity and CDK1-CyclinB interaction with centriolar components, ensure that centriole biogenesis

18 bin-depleted cells restored the cellular and centriolar CPAP expression, suggesting its ubiquitinatio

19 in-depleted cells led to the reappearance of centriolar CPAP.

20 somal protein 4.1 epitopes distributed along centriolar cylinders and on pericentriolar fibers, at le

21 our knowledge, we have identified the first centriolar deubiquitinating enzyme whose expression regu

22 and is required for the recruitment of five centriolar distal appendage proteins: Sclt1, Ccdc41, Cep

23 lium biogenesis by promoting the assembly of centriolar distal appendages critical for docking ciliar

24 This extra centriolar DSas-6/Ana2 induces centriole overduplication

25 rting endosomes and accumulation in the peri-centriolar endocytic recycling compartment takes place n

26 and promotes the generation of supernumerary centriolar foci, whereas ablation of USP33 destabilizes

27 ison to cells lacking Ift88 reveals that the centriolar functions of Kif3a are independent of IFT.

28 epletion of Cep76 drives the accumulation of centriolar intermediates in certain types of cancer cell

29 letion of Tssk1 and 2 (companion paper) this centriolar kinase/substrate pair is predicted to play an

30 Thus, our studies have identified a centriolar kinesin that specifically remodels a subset o

31 omal proteins that play a role in regulating centriolar length and ciliogenesis.

32 bin from cells did not have an effect on the centriolar levels of CEP152, it caused the disappearance

33 ain, a C-terminal dimerization domain, and a centriolar localization domain.

34 In addition to the centriolar localization during flagellogenesis, mouse TS

35 We confirmed centriolar localization for the human homologs of four c

36 es SPD-2 centriolar recruitment, while SAS-7 centriolar localization is SPD-2-independent.

37 resses centriole duplication by limiting the centriolar localization of CEP135(full) binding proteins

38 Notably, the centriolar localization of FAM92A and -92B depends large

39 ughter centriole formation and regulates the centriolar localization of the other components in C. el

40 This centriolar localization persisted in ejaculated human sp

41 However, the molecular basis for SPD-2 centriolar localization remains unknown.

42 The clear absence of several centriolar markers in mecD mutants suggests that Asl is

43 Using fluorescent centriolar markers, we identified a structure near the f

44 tal appendages, recruit Ift88, and stabilize centriolar microtubules at a defined length.

45 ond the distal end of the centriole, as some centriolar microtubules can be more than 50 times longer

46 Second, CP110 ensures that the centriolar microtubules do not extend beyond the distal

47 4, which is required for the assembly of the centriolar microtubules that decorate that tube [4, 5].

48 A also acts as a microtubule depolymerase on centriolar microtubules to regulate centriole length.

49 ically expressed Kif24 specifically remodels centriolar microtubules without significantly altering c

50 Although Plk4's centriolar partners and mechanisms that regulate its sta

51 PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplic

52 not affect Fz/PCP establishment, implicating centriolar positioning as a conserved PCP-readout, likel

53 e splitting reduces the local density of key centriolar precursors to impede overduplication.

54 Here we show that the centriolar protein Asterless (Asl; human orthologue CEP1

55 three Mps1 phosphorylation sites within the centriolar protein Centrin 2 (Cetn2).

56 new study has shed light on the role of the centriolar protein centrin-2: reducing levels of centrin

57 The centriolar protein Cp110 is a regulator of this process

58 ied a rare missense variant (p.A446T) in the centriolar protein gene POC5 that cosegregated with the

59 lethalus syndrome protein HYLS-1 is the only centriolar protein known to remain at the base of mature

60 We identified the mother centriolar protein ODF2 as an interaction partner of MAR

61 mutation in the gene encoding the conserved centriolar protein POC1, which is part of the daughter c

62 reas the carboxy terminus interacts with the centriolar protein Sas-4 (CPAP in humans).

63 ch MTOCs had pericentriolar material and the centriolar protein Sas-4, but no centrioles at their cor

64 ased on its direct interaction with the core centriolar protein SAS-4.

65 icrocephaly protein CenpJ and the C. elegans centriolar protein Sas-4.

66 ication involves self-oligomerization of the centriolar protein SAS-6, but how the 9-fold symmetry is

67 rganized through homo-oligomerization of the centriolar protein SAS-6, but whether SAS-6 self-assembl

68 embly, involving self-oligomerization of the centriolar protein SAS-6.

69 post-transcriptional repression of Cp110, a centriolar protein suppressing cilia assembly.

70 tii Bld10p and human Cep135, is a ubiquitous centriolar protein that also localizes to the spermatid

71 We have identified Cep76, a centriolar protein that interacts with CP110.

72 oles, defining the first stably incorporated centriolar protein that is not required for centriole as

73 uplication is controlled in part by CP110, a centriolar protein that positively regulates centriole d

74 We use this assay to characterize SAS-6, a centriolar protein that we identified based on its requi

75 se haplotype in CEP120, which encodes a core centriolar protein.

76 in-containing protein TbRP2 is a basal body (centriolar) protein essential for axoneme formation in t

77 Thus, Ana3 defines a conserved family of centriolar proteins and plays an important part in ensur

78 driven by an interaction with the conserved centriolar proteins Asl (Cep152 in humans) and DSpd-2 (C

79 pathogenesis of JATD and expand the role of centriolar proteins in skeletal ciliopathies.

80 to calculate the average toroidal radius of centriolar proteins in the approximately 20-60 nm range

81 ggesting that Cetn2 can organize a subset of centriolar proteins independently of cartwheels.

82 ic cells, however knowledge regarding gamete centriolar proteins is limited.

83 Second, the centriolar proteins SAS-6, Ana1, and Bld10p/Cep135 are i

84 functions for these important basal body and centriolar proteins.

85 t of mitosis through phosphorylation of core centriolar proteins.

86 for centriole distal appendage formation and centriolar recruitment of the intraflagellar transport p

87 w that SAS-7 binds SPD-2 and regulates SPD-2 centriolar recruitment, while SAS-7 centriolar localizat

88 ophages lack a morphologically distinct peri-centriolar recycling compartment but instead demonstrate

89 LIP-50 protein localizes specifically to the centriolar region where the sperm tail originates and to

90 uggested that pharmacological stimulation of centriolar reproduction without subsequent mitosis may l

91 Centriolar SAS-4 remains in dynamic equilibrium with the

92 Centriolar SAS-6 is subsequently reduced by a mechanism

93 iary vesicle formation through regulation of centriolar satellite accretion and Rab8a.

94 in centriole duplication, ciliogenesis, and centriolar satellite biogenesis and highlights extensive

95 These results suggest that FOP is a centriolar satellite cargo protein and, as for several o

96 The centriolar satellite component PCM-1 colocalized with ce

97 entrosome including microtubule binding, the centriolar satellite component PCM-1, and localized prot

98 analyses showed that Talpid3 is required for centriolar satellite dispersal, which precedes the forma

99 GABARAP instability is mediated through the centriolar satellite E3 ligase Mib1, which interacts wit

100 ired for ciliogenesis but is dispensable for centriolar satellite function.

101 nt manner; however, C2cd3 is dispensable for centriolar satellite integrity.

102 ics with pericentriolar material 1 (PCM1), a centriolar satellite protein crucial for targeting prote

103 entrosomal activity or downregulation of the centriolar satellite protein PCM-1 affects axon formatio

104 olar satellites where it interacted with the centriolar satellite protein PCM-1 and the dynactin subu

105 we demonstrate that CEP290 interacts with a centriolar satellite protein PCM-1, which is implicated

106 ma-tubulin ring complex (gamma-TuRC) and the centriolar satellite protein PCM-1.

107 We show that the centriolar satellite protein PCM1 regulates the recruitm

108 Here we characterize the function of a novel centriolar satellite protein, synovial sarcoma X breakpo

109 nown centrosome protein with homology to the centriolar satellite proteins FOR20 and OFD1.

110 vealed extensive proximity interactions with centriolar satellite proteins.

111 MCPH proteins interact with distinct centriolar satellite proteins; CDK5RAP2 interacts with S

112 y and function of motile cilia and implicate centriolar satellite-associated proteins as a new class

113 calization occurs normally in the absence of centriolar satellites (PCM1 depletion) but is impaired b

114 les colocalize in part with PCM-1 containing centriolar satellites and depletion of PCM-1 interferes

115 /SSX2IP localizes to both the centrosome and centriolar satellites and is required for tethering micr

116 ignificance of spatial communication between centriolar satellites and the centrosome is unknown.

117 Centriolar satellites are centrosome-associated structur

118 Our data suggest that PCM-1-containing centriolar satellites are involved in the microtubule- a

119 Centriolar satellites are numerous electron-dense granul

120 Centriolar satellites are proteinaceous granules that ar

121 On hMsd1/SSX2IP knockdown, the centriolar satellites become stuck at the microtubule mi

122 Thus, centriolar satellites build a MCPH complex critical for

123 Loss of centriolar satellites by depletion of PCM1 causes reloca

124 ep72, and Cep290 and find that disruption of centriolar satellites by overexpression of Cep72 results

125 These data suggest a mechanism for how centriolar satellites can specifically regulate an ATG8

126 Furthermore, PCM1-GABARAP-positive centriolar satellites colocalize with forming autophagos

127 These results suggest that centriolar satellites have a previously unappreciated fu

128 Although centriolar satellites have been implicated in protein tr

129 We show that C2cd3 is localized to the centriolar satellites in a microtubule- and Pcm1-depende

130 CEP290 binds to PCM-1 and localizes to centriolar satellites in a PCM-1- and microtubule-depend

131 To elucidate the role of centriolar satellites in ciliogenesis, we deleted the ge

132 plex, the BBSome, localizes to nonmembranous centriolar satellites in the cytoplasm but also to the m

133 Finally, we show that loss of centriolar satellites in zebrafish leads to phenotypes c

134 pid3 resulted in an aberrant distribution of centriolar satellites involved in protein trafficking to

135 We show that the population of OFD1 at the centriolar satellites is rapidly degraded by autophagy u

136 calization of the FOP-FGFR1 fusion kinase to centriolar satellites may be relevant to myeloproliferat

137 some, suggesting that their association with centriolar satellites normally restricts their centrosom

138 More strikingly, OFD1 depletion at centriolar satellites promotes cilia formation in both c

139 n, OFD1 (oral-facial-digital syndrome 1), at centriolar satellites promotes primary cilium biogenesis

140 We propose that Cep290 and Cep72 in centriolar satellites regulate the ciliary localization

141 reveals that removal of OFD1 by autophagy at centriolar satellites represents a general mechanism to

142 During ciliogenesis, BBS4 relocalizes from centriolar satellites to the primary cilium.

143 eloproliferative neoplasm, also localizes to centriolar satellites where it increases tyrosine phosph

144 We detected Par6alpha at the centrosome and centriolar satellites where it interacted with the centr

145 localizes to cytoplasmic granules known as "centriolar satellites" that are partly enriched around t

146 e embryonic fibroblasts, OFD1 accumulates at centriolar satellites, leading to fewer and shorter prim

147 cells also have an array of granules, termed centriolar satellites, that localize around the centroso

148 ation, accompanied by reduced density of the centriolar satellites, with reexpression of C-NAP1 rescu

149 onstrate that Ccdc11 is a novel component of centriolar satellites-cytoplasmic granules that serve as

150 al, GABARAP marks a subtype of PCM1-positive centriolar satellites.

151 rity, centrosome length and orientation, and centriolar satellites.

152 kdown that reduces the population of OFD1 at centriolar satellites.

153 the ciliopathy-associated protein Cep290 to centriolar satellites.

154 We show that centriolar singlet microtubules are converted into BB do

155 centriole, which may explain why no typical centriolar structure is observed under electron microsco

156 o the periphery of the centrosome but not at centriolar structures as in mammals.

157 isted in ejaculated human spermatozoa, while centriolar TSKS diminished in mouse sperm, where centrio

158 , which are necessary to form the C. elegans centriolar tube, a scaffold in centriole formation [4, 5

159 flagellar assembly function of specialized, centriolar tubulin cofactor C domain-containing proteins