ライフサイエンスコーパス: centriole

1 ntrioles is guided by a pre-existing, mother centriole.

2 and that the PCL does not resemble a typical centriole.

3 and templates the formation of the daughter centriole.

4 vity promotes the recruitment of STIL to the centriole.

5 t to block further duplication of the mother centriole.

6 CM outfitting the proximal end of the mother centriole.

7 ent regulation of CP110 levels at the mother centriole.

8 ed pericentriolar material around the mother centriole.

9 eins from satellites and their relocation to centrioles.

10 ually a highly organised scaffold around the centrioles.

11 ligase to satellites and restricting it from centrioles.

12 , whereas depletion of Cetn3 generates extra centrioles.

13 basal bodies, plasma membrane-docked mother centrioles.

14 wever, that normal rootlet assembly requires centrioles.

15 iogenesis in cells with manipulated daughter centrioles.

16 t cilia maturation does not depend on intact centrioles.

17 ilia or the closely associated distal end of centrioles.

18 rtwheel structure that comprises the base of centrioles [6-11].

19 Centrioles acquire subdistal appendages (sDAPs) during p

20 ntriole-PCM interaction in a cell-cycle- and centriole-age-dependent manner.

21 es maturation and distancing of the daughter centriole, allowing reduplication of the mother centriol

22 not all, eukaryotes and are associated with centrioles, although their molecular function is unclear

23 udy has implicated the daughter centriole in centriole amplification in multiciliated cells, but its

24 and E2F4 mutant proteins we demonstrate that centriole amplification is crucially dependent on these

25 However, how Asl overexpression drives centriole amplification is unknown.

26 ly of cytoplasmic structures for large-scale centriole amplification that generates basal bodies.

27 erved centriole assembly factors that induce centriole amplification when overexpressed.

28 previously unsuspected cytoplasmic events of centriole amplification, providing new perspectives for

29 promoting its own degradation and preventing centriole amplification.

30 e duplication, and its hyperactivity induces centriole amplification.

31 r RSG1 in the final maturation of the mother centriole and ciliary vesicle that allows extension of t

32 m localize to the proximal end of the mother centriole and interact physically, with Wdr62 required f

33 10 (CP110) caps the distal end of the mother centriole and is known to act as a suppressor to control

34 that BCCIP localizes proximal to the mother centriole and participates in microtubule organization a

35 lays important roles in the orchestration of centriole and PCM assembly.

36 ciliated species, associates with centrosome/centriole and pericentriolar satellites in human cells a

37 uired for the interaction between the mother centriole and the PCM.

38 polyglutamylated tubulin (PGT) on the mother centriole and with PCNT in the PCM renders ATF5 as a req

39 3 attenuates the incorporation of Cetn2 into centrioles and centrosome reduplication, whereas depleti

40 ubulin in cycling cells does not localize to centrioles and is associated with the TRiC/CCT cytoplasm

41 ing site that helps recruit Polo to daughter centrioles and is required for the subsequent recruitmen

42 that are assembled from paternally inherited centrioles and maternally inherited pericentriolar mater

43 causes the formation of multiple numbers of centrioles and multipolar spindles with abnormal chromos

44 Girdin localizes to the proximal regions of centrioles and regulates BB positioning and ciliogenesis

45 1A encodes a WD repeat protein localizing to centrioles and spindle poles and is associated with shor

46 tubulin facilitates interactions between the centrioles and the apical cytoskeleton as a component of

47 tion of distal appendage markers on daughter centrioles and the loss of procentriolar markers.

48 DZIP1L localizes to centrioles and to the distal ends of basal bodies, and i

49 In the absence of F-actin, the sperm DNA, centrioles, and organelles were transported as a unit wi

50 lular structures such as the nucleus, Golgi, centrioles, and spindle show remarkable diversity betwee

51 Together, our results reveal links among centrioles, apical proteins, and cell fate, and illumina

52 These sperm centrioles appear as vestigial basal bodies, destroyed i

53 Centrioles are ancient organelles that build centrosomes

54 n mechanisms are necessary because if mother centrioles are artificially retained, they cannot be ina

55 Centrioles are composed of long-lived microtubules arran

56 How centrioles are eliminated remains poorly understood.

57 Centrioles are essential for the assembly of both centro

58 en together, our results indicate that while centrioles are essential to initiate cilia formation, th

59 These aberrant centrioles are formed de novo each cell cycle, but are u

60 Centrioles are microtubule-based organelles crucial for

61 undergo reduplication when original daughter centrioles are only approximately 80 nm apart, which is

62 We show that centrioles are polarized in pupal wing cells as a readou

63 r, these findings demonstrate that the sperm centrioles are remodeled both in their protein compositi

64 s, we demonstrate that the two older, mother centrioles are selectively removed from the oocyte by ex

65 Centrioles are small barrel-shaped structures that form

66 Centrioles are the foundation of two organelles, centros

67 pd-2 and Asl are not recruited to the mother centriole as part of a complex with Sas-4.

68 st, of the distal and, then, of the proximal centriole as the sperm transits to the cauda and vas def

69 Centrioles assemble around a central cartwheel comprisin

70 icrotubule anchoring is essential for proper centriole assembly and duplication.

71 provide novel insights into the mechanism of centriole assembly and microtubule anchoring.

72 ner Asterless (Asl) are essential, conserved centriole assembly factors that induce centriole amplifi

73 d and phosphorylate STIL in G1, allowing pro-centriole assembly in the subsequent S phase.

74 subsequently phosphorylates STIL to promote centriole assembly in two steps.

75 mo-oligomerisation in vitro strongly perturb centriole assembly in vivo.

76 cartwheel, and SAS-6 levels remain low until centriole assembly is initiated at S phase onset [3, 12,

77 but how its activity is regulated to control centriole assembly is unclear.

78 trosome disruption by chemical prevention of centriole assembly or genetic ablation of pericentrin at

79 rine-threonine protein kinase that initiates centriole assembly.

80 se in CEP135(mini) from centrosomes promotes centriole assembly.

81 be caused by effects on Neurl-4, a daughter centriole-associated ubiquitin ligase cofactor, which wa

82 tebrate cells can initiate ciliogenesis from centrioles at the cell center, near the Golgi, forming p

83 nsuring the formation of the right number of centrioles at the right time.

84 wed that MDM1 is closely associated with the centriole barrel, likely residing in the centriole lumen

85 d interaction between the cell cycle and its centriole-basal body-flagellar cycle.

86 The centriole/basal body is a eukaryotic organelle that play

87 The evolutionarily conserved centriole/basal body protein SAS-4 regulates centriole d

88 served coiled-coil protein that localizes to centrioles/basal bodies and plays a crucial role in the

89 l lines, FAM92A colocalizes with Cby1 at the centrioles/basal bodies of primary cilia, while FAM92B i

90 tein, Spd-2, are recruited around the mother centriole before spreading outwards to form a scaffold t

91 an overview of our current understanding of centriole biogenesis and how variations around the same

92 tion with centriolar components, ensure that centriole biogenesis occurs once and only once per cell

93 Centriole biogenesis occurs once and only once per cell

94 n early mitosis, at a time when triggers for centriole biogenesis Polo-like kinase 4 (PLK4) and its s

95 ike kinase 4 (Plk4) is a master regulator of centriole biogenesis, but how its activity is regulated

96 After inducing a transcriptional program of centriole biogenesis, E2f4 forms apical cytoplasmic orga

97 n by PLK4, thus inhibiting untimely onset of centriole biogenesis.

98 -like kinase 4 (Plk4), a master regulator of centriole biogenesis.

99 ue homeostasis, are extensions of the mother centriole, but the mechanisms that remodel the centriole

100 de up of rootletin filaments anchored to the centrioles by C-NAP1.

101 These centrioles can neither recruit gamma-tubulin nor nucleat

102 We find that mother centrioles can undergo reduplication when original daugh

103 s, we surprisingly found that normal looking centrioles capable of duplication and ciliation can aris

104 a, these results suggest a unifying model of centriole/cilia positioning as a common downstream effec

105 on in vertebrates is to regulate coordinated centriole/cilia positioning, a function that has not bee

106 losed that AKAP350A spans the bridge between centrioles, co-localizing with rootletin and Cep68 in th

107 es Cep135 to properly position the essential centriole component Asterless.

108 Here, we describe a new centriole component, the coiled-coil protein SAS-7, as a

109 resolution microscopy, and identification of centriole components have accelerated our understanding

110 sis, the distal appendages of the CTL mother centriole contact the plasma membrane directly during sy

111 meiotic spindle assembles in the absence of centriole-containing centrosomes.

112 gagement during mitosis, but block efficient centriole conversion and lead to embryonic lethality.

113 It is unclear how this mitotic "centriole conversion" is regulated, but it requires Plk1

114 liary axoneme, visualize degeneration of the centriole core, and define the developmental stage at wh

115 Discovery of the centriole dates back to the 19th century.

116 ep290-deficient mice exhibited supernumerary centrioles, decreased replication fork velocity, fork as

117 nek2 knockdown leads to a centriole defect at the LRO, consistent with the known r

118 oscopy and electron tomography, we find that centrioles degenerate early during ciliogenesis.

119 c tools that allow persistent and reversible centriole depletion.

120 ia are cellular projections that assemble on centriole-derived basal bodies.

121 he primary cilium is nucleated by the mother centriole-derived basal body (BB) via as yet poorly char

122 enders ATF5 as a required molecule in mother centriole-directed PCM accumulation and in PCM-dependent

123 Centrosome fragmentation and precocious centriole disengagement depend on separase and anaphase-

124 phosphorylation or Polo docking do not block centriole disengagement during mitosis, but block effici

125 he initiation of centriole licensing through centriole disengagement, at which point the ability to m

126 ntercellular bridges (fusomes) and premature centriole disengagement.

127 ent increase in centrosome fragmentation and centriole disengagement.

128 In contrast, the essential mother centriole distal appendage protein CEP164 did not play a

129 del with mutated Sclt1 gene, which encodes a centriole distal appendage protein important for cilioge

130 onvert small vesicles associated with mother centriole distal appendages into a larger ciliary vesicl

131 0 are known to regulate the integrity of the centriole distal end, confirming that this structural el

132 f zeta-tubulin results in disorganization of centriole distribution and polarity in multiciliated cel

133 ase plate position by creating an asymmetric centriole distribution on each pole, we find that metaph

134 wo half-spindles in cells with an asymmetric centriole distribution.

135 que opportunity to address this question, as centrioles do not persist at the base of mature cilia.

136 naling structure that arises from the mother centriole docked to the cell membrane, was intact in the

137 Importantly, loss or gain of centrioles does not affect Fz/PCP establishment, implica

138 n of centrobin causes abnormal elongation of centrioles due to massive accumulation of CPAP in the ce

139 Cellular checkpoints ensure that the centrioles duplicate only once in every cell cycle and a

140 We found that they interact to promote centriole duplication and form a hierarchy in which each

141 K4), that plays a well-characterized role in centriole duplication and has the ability to alter mitot

142 of Asterless (Asl), a protein essential for centriole duplication and mitotic centrosome assembly.

143 propose that MDM1 is a negative regulator of centriole duplication and that its function is mediated

144 The repression of centriole duplication by a splice isoform of a protein t

145 ception of CCDC14, and MCPH proteins promote centriole duplication by recruiting CDK2 to the centroso

146 Insights into the role of Asl/Cep152 beyond centriole duplication could help shed light on how Cep15

147 r62, Aspm, or both showing gene dose-related centriole duplication defects that parallel the severity

148 Disruption of either domain leads to centriole duplication failure in worm embryos, indicatin

149 instability by preventing cell growth after centriole duplication failure.

150 Thus, efficient centriole duplication in flies requires the homo-oligome

151 centriole/basal body protein SAS-4 regulates centriole duplication in metazoa and basal body duplicat

152 Centriole duplication is coordinated such that a single

153 Centriole duplication occurs once per cell cycle in orde

154 gulate the number of centrosomes by limiting centriole duplication to once per cell cycle.

155 Depleting p53 allowed cells that fail centriole duplication to proliferate indefinitely.

156 that SAS-7 functions at the earliest step in centriole duplication yet identified and plays important

157 ike kinase 4 (Plk4) is a master regulator of centriole duplication, and its hyperactivity induces cen

158 coiled-coil protein SAS-7, as a regulator of centriole duplication, assembly and elongation.

159 N-terminal region of Asl is not required for centriole duplication, but a previously unidentified Plk

160 ughter centriole, established at the time of centriole duplication, is thought to block further dupli

161 Overexpression of MDM1 suppressed centriole duplication, whereas depletion of MDM1 resulte

162 melanogaster orthologue of Cep152, prevents centriole duplication, which has limited the study of it

163 sl affects Plk4 in multiple ways to regulate centriole duplication.

164 ) and targets Plk4 to centrioles to initiate centriole duplication.

165 otes it serves as a novel mechanism to limit centriole duplication.

166 d cytokinesis; PLK4 is a master regulator of centriole duplication.

167 n of centriolar PLK4 and plays a key role in centriole duplication.

168 t promotes CDK2 centrosomal localization and centriole duplication.

169 novel role for Plk4, the master regulator of centriole duplication.

170 EP135 that antagonize each other to modulate centriole duplication: full-length CEP135 (CEP135(full))

171 The localization of Fzr to the centriole during interphase depends on direct interactio

172 Most oocytes eliminate their centrioles during meiotic divisions through unclear mech

173 enrichment is essential for the formation of centrioles during spermiogenesis and for the formation o

174 have begun to shed light on the mechanism of centriole elimination during female oogenesis, highlight

175 Centriole elimination is an essential process that occur

176 CPAP and prevents its degradation for normal centriole elongation function.

177 f the cell cycle, centrosomes consist of two centrioles embedded in the proteinaceous pericentriolar

178 is essential for normal fusome behavior and centriole engagement during premeiotic G2 arrest of Dros

179 orientation between a mother and a daughter centriole, established at the time of centriole duplicat

180 triole, allowing reduplication of the mother centriole even if the original daughter centriole is sti

181 Without Cby and Azi1, centrioles fail to form the TZ, precluding sensory cilia

182 ext cell cycle, leading to a futile cycle of centriole formation and disintegration.

183 und the same theme generate alternatives for centriole formation and function.

184 the first recruited to the site of daughter centriole formation and regulates the centriolar localiz

185 ibution of triplet microtubules to mammalian centriole formation and stability is unknown.

186 itecture of SAS-6 and its role in initiating centriole formation are well understood, the mechanisms

187 Consistently, centriole formation is thought to strictly rely on self-

188 und that manipulations that prevent daughter centriole formation or induce its separation from the mo

189 In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5

190 hter centrioles was proposed to restrict new centriole formation until they separate beyond a critica

191 bly and that the PCM is essential for proper centriole formation, the mechanism that governs centriol

192 itive to Plk4 overproduction-induced ectopic centriole formation, yet they accelerate centrosome redu

193 rected PCM accumulation and in PCM-dependent centriole formation.

194 hat likely represents early intermediates in centriole formation.

195 -testicular sperm maturation, in which sperm centrioles found in the caput are destroyed prior to eja

196 that these are necessary for inheritance of centrioles from one cell cycle to the next.

197 ila PCM-organising proteins are recruited to centrioles from the cytosol as part of large cytoplasmic

198 Centriole function has been difficult to study because o

199 pose that the ZED tubulins are important for centriole functionalization and orientation of centriole

200 how that the two sperm centrioles (the giant centriole [GC] and the proximal centriole-like structure

201 The centriole has a dual life, existing not only as the core

202 These observations can explain why daughter centrioles have to pass through mitosis before they can

203 RSG1 localizes to the mother centriole in a process that depends on tau tubulin kinas

204 url-4 transiently associated with the mother centriole in a process that required mother-daughter cen

205 A recent study has implicated the daughter centriole in centriole amplification in multiciliated ce

206 tion [12], remain associated with the mother centriole in CTLs, and neither axoneme nor transition zo

207 Here, we report that centrioles in delta-tubulin and epsilon-tubulin null mut

208 identify populations of cells with Asl-free centrioles in developing Drosophila tissues, allowing us

209 ed reduction of cilia and abnormal number of centrioles in fibroblasts from one affected individual.

210 expressed Root resides at the base of mother centrioles in spermatocytes and localizes asymmetrically

211 Microtubule-based centrioles in the centrosome mediate accurate bipolar ce

212 henomenon of Poc1 enrichment of the atypical centrioles in the spermatozoa.

213 n by regulating the conversion of the mother centriole into the cilia basal body.

214 The centriole is a multifunctional structure that organizes

215 The centriole is an evolutionarily conserved structure built

216 ther centriole even if the original daughter centriole is still orthogonal to it.

217 The formation of new daughter centrioles is guided by a pre-existing, mother centriole

218 structure of the matured sperm (spermatozoa) centrioles is modified dramatically and that the PCL doe

219 le cilia assembly is absolutely dependent on centrioles, it is not known to what extent they contribu

220 t the centrosome and causes fragmentation of centrioles, leading to the formation of multi-polar mito

221 of endogenous Plk4 levels in cells that lack centrioles led to the penetrant formation of de novo cen

222 and triggers its degradation to restrict the centriole length during biogenesis.

223 We show a role for Asl in controlling centriole length in germline and somatic tissue, functio

224 itotic progression trigger the initiation of centriole licensing through centriole disengagement, at

225 s (the giant centriole [GC] and the proximal centriole-like structure [PCL]) in Drosophila melanogast

226 In other animals, however, sperm centrioles lose their proteins and are thought to be deg

227 the centriole barrel, likely residing in the centriole lumen.

228 While most cells with disengaged centrioles maintain spindle bipolarity, clustering of di

229 ack triplet microtubules and fail to undergo centriole maturation.

230 Although the mother centriole mediates most centrosome-dependent processes,

231 conserved centriole protein dynamically caps centriole microtubule plus ends to limit the organelle's

232 indings reveal a dual mechanism to eliminate centrioles: mothers are physically removed, whereas daug

233 issue, Borrego-Pinto et al. show that mother centrioles need to be eliminated from starfish oocytes b

234 oximately 80 nm apart, which is the distance centrioles normally reach during prophase.

235 Defects in centriole number and structure are associated with human

236 occurs in female meiosis of metazoa to reset centriole number in the zygote at fertilization.

237 However, centriole number, size, and organization varies among di

238 s with CDK inhibitors rescued DNA damage and centriole number.

239 Centriole numbers and functions are tightly controlled,

240 Centriole numbers are tightly regulated, and daughter ce

241 e we show that the MDM1 protein localizes to centrioles of dividing cells and differentiating multici

242 The two centrioles of the centrosome differ in age and function.

243 domains that mediate preferential binding to centrioles over rootlets.

244 Paradoxically, mother and daughter centrioles overcome this distance in early mitosis, at a

245 Centrosomes and cilia are organized by a centriole pair comprising an older mother and a younger

246 nd controls demonstrate both the presence of centriole pairs in the upper caput region of the epididy

247 an occur in proliferating CTLs with multiple centriole pairs.

248 ATF5 controls the centriole-PCM interaction in a cell-cycle- and centriole

249 triole formation, the mechanism that governs centriole-PCM interaction is poorly understood.

250 Centrioles play a key role in the development of the fly

251 Although it is known that the centrioles play instructive roles in pericentriolar mate

252 loss-of-function Fz/PCP signalling affecting centriole polarization.

253 zzled-PCP (Fz/PCP) signalling into polarized centriole positioning in Drosophila wings.

254 We conclude that pro-centrioles/pro-basal bodies are multipotent and not comm

255 r data support a model in which the daughter centriole promotes ciliogenesis through Neurl-4-dependen

256 Loss of the centriole protein Asterless (Asl), the Drosophila melano

257 line and somatic tissue, functioning via the centriole protein Cep97.

258 Sharma et al. (2016) report how a conserved centriole protein dynamically caps centriole microtubule

259 in flies, Cdk1 phosphorylates the conserved centriole protein Sas-4 during mitosis.

260 toplasmic 'S-CAP' complexes that contain the centriole protein Sas-4.

261 cated paralogue of the ubiquitous basal body/centriole protein SAS6, has been characterised recently

262 Several centriole proteins are distributed in a toroid around th

263 ons are tightly controlled, and mutations in centriole proteins are linked to a variety of diseases,

264 Among five known core centriole proteins, SPD-2/Cep192 is the first recruited

265 ertheless, these induced MTNCs lacked "true" centriole proteins, such as Cep135.

266 t a group of sDAP components localize to the centriole proximal end through the cohesion factor C-Nap

267 ementary mechanisms, such as mother-daughter centriole proximity and CDK1-CyclinB interaction with ce

268 e in a process that required mother-daughter centriole proximity.

269 Rsg1 mother centrioles recruit proteins required for cilia initiatio

270 The mother centriole recruits and organises the PCM and templates t

271 ted in the testes, here we demonstrate sperm centriole reduction occurs within the epididymis.

272 In contrast, the single daughter centriole remaining in the egg is eliminated before the

273 we demonstrate that STIL localization to the centriole requires continued Plk4 activity.

274 spindle bipolarity, clustering of disengaged centrioles requires the kinesin-14, HSET.

275 and had an increased frequency of premature centriole separation, accompanied by reduced density of

276 O, consistent with the known role of Nek2 in centriole separation.

277 We conclude that centriole splitting reduces the local density of key cen

278 ron microscopy, that under these conditions, centriole structures are faulty.

279 onal culture systems, we identified a mother centriole subdistal appendage protein, cenexin, as a cri

280 Here, by characterizing centriole subdistal appendages (sDAP) in cells exclusive

281 arrays within animal cells and comprises two centrioles surrounded by an amorphous protein mass calle

282 es led to the penetrant formation of de novo centrioles that gained the ability to organize microtubu

283 Here, we show that the two sperm centrioles (the giant centriole [GC] and the proximal ce

284 Thus the structure and functions of the centriole, the centrosome, and the basal body have an im

285 we review the structure and functions of the centriole, the centrosome, and the basal body in differe

286 epithelial cells, and despite having intact centrioles, they were unable to make cilia upon serum st

287 Cep97, which must dissociate from the mother centriole to allow cilia formation [12], remain associat

288 ntriole, but the mechanisms that remodel the centriole to promote cilia initiation are poorly underst

289 riven transport, and anchorage of the mother centriole to the plasma membrane via mother-specific app

290 suggest that SAS-7 is required for daughter centrioles to become competent for duplication, and for

291 rotein called SAS-7 is required for daughter centrioles to become mothers in C. elegans.

292 tment of small vesicles at the distal end of centrioles to facilitate basal body docking to the plasm

293 o box cassette (PB1-PB2) and targets Plk4 to centrioles to initiate centriole duplication.

294 me competent for duplication, and for mother centrioles to maintain this competence.

295 l foot, a centriolar appendage that connects centrioles to the apical cytoskeleton, and co-localizes

296 Upon cell cycle exit, centriole-to-basal body transition facilitates cilia for

297 The proximity between mother and daughter centrioles was proposed to restrict new centriole format

298 that active JAK2 localizes around the mother centrioles, where it partly colocalizes with ninein, a p

299 numbers are tightly regulated, and daughter centrioles (which assemble in S phase) cannot themselves

300 ntriole functionalization and orientation of centrioles with respect to cellular polarity axes.

301 In some animals, sperm centrioles with typical ultrastructure are the origin of